A cloning kit plasmid extraction column

Abstract

The utility model discloses a cloning kit plasmid extraction column, and particularly relates to the field of experimental supplies. A cloning kit plasmid extraction column, which comprises a filter core, further comprises a column body, the filter core and the inner wall of column body are in sliding fit, the outer wall of column body is provided with a limiting sleeve ring and a fixed sleeve ring, the top of limiting sleeve ring and the bottom of fixed sleeve ring are all provided with a plurality of insertion slots, and the outer wall of column body is provided with a sealing assembly. Through the mutual cooperation between the column body, the limiting sleeve ring, the fixed sleeve ring and the sliding sleeve ring, the sliding sleeve ring can slide on the outer wall of the column body, and then the sliding distance is limited by the limiting sleeve ring and the fixed sleeve ring. The position of the cover plate can be adjusted according to whether the filter core is installed in the column body or not, so that the bending piece can adapt to different occasions, excessive bending of the bending piece is avoided, the cover plate is prevented from being automatically opened due to excessive pressure during the sealing process, and the experimental work is affected, and the applicability is strong.

Description

Technical Field

[0001] This utility model relates to the field of laboratory supplies, and in particular to a plasmid extraction column for a cloning kit. Background Technology

[0002] In molecular biology research and genetic engineering applications, plasmids serve as exogenous gene vectors. The efficiency and purity of their extraction and purification directly determine the success or failure of subsequent experiments such as clone construction, cell transfection, and protein expression. As a standardized experimental tool, the cloning kit contains a plasmid extraction column, which is the core component for achieving bacterial lysis, impurity separation, and plasmid enrichment. It needs to be adapted to the processing requirements of different bacterial volumes and be compatible with conventional chemical lysis and subsequent elution processes. Plasmid extraction columns are required during the experiment.

[0003] The existing plasmid extraction column's cover plate and column body connection structure is mostly designed with a fixed angle and a single height. It cannot flexibly adjust the cover plate position according to whether a filter element is installed in the column. Frequent excessive bending will lead to elastic fatigue, which not only easily produces irreversible cracks, but also causes the cover plate to accidentally pop open due to rebound stress during experiments such as centrifugation and transfer, thus affecting the experimental work and having poor applicability. Utility Model Content

[0004] The main purpose of this invention is to provide a plasmid extraction column for a cloning kit, which can effectively solve the problem of poor adaptability of the cover plate and insufficient sealing of the column after frequent and excessive bending.

[0005] To achieve the above objectives, the technical solution adopted by this utility model is as follows: A plasmid extraction column for a cloning kit includes a filter element and a column body. The filter element slides within the inner wall of the column body. The outer wall of the column body is provided with a limiting collar and a fixing collar. The top of the limiting collar and the bottom of the fixing collar are each provided with a plurality of slots. The outer wall of the column body is provided with a sealing assembly, which includes a sliding collar, a bending member, a cover plate, and a sealing tube. Both ends of the sliding collar are fixedly mounted with a plurality of plugs, which can slide within the slots of the limiting collar or the fixing collar, respectively. Both ends of the bending member are fixedly connected to the sliding collar and the cover plate, respectively. The bottom of the cover plate fits against the top of the column body and the filter element. The sealing tube slides within the inner wall of the column body or the filter element. The bottom of the column body is provided with a conical head.

[0006] Preferably, both the slot and the plug are pear-shaped, and the slot, the plug, and the sealing tube are all made of rubber.

[0007] Preferably, the sliding collar slides in contact with the outer wall of the column.

[0008] Preferably, the inner wall of the bent component has two protrusions.

[0009] Preferably, the bottom of the cover plate is provided with stripes, and the top is provided with a groove.

[0010] Preferably, the sealing tube is fixedly installed at the bottom of the cover plate, and the diameter of the sealing tube and the inner wall diameter of the column are adapted to each other.

[0011] Preferably, the outer wall of the sealing tube has rounded corners.

[0012] Compared with the prior art, the present invention has the following beneficial effects: Through the cooperation between the column, limiting collar, fixed collar, and sliding collar, the sliding collar can slide on the outer wall of the column, and then the sliding distance is limited by the limiting collar and fixed collar. The position of the cover plate can be adjusted according to whether a filter element is installed inside the column, so that the bent part can adapt to different occasions, avoid excessive bending of the bent part, and prevent the cover plate from opening automatically due to excessive pressure during the sealing process, which would affect the experimental work. It has strong applicability. Through the cooperation between the slot and the plug, the sliding collar can allow the plugs on both sides to be inserted into the slots opened on one side of the limiting collar and the fixed collar respectively for limiting when it moves vertically, which effectively improves the stability of the sealing assembly after movement and further improves its applicability. Attached Figure Description

[0013] Figure 1 This is a three-dimensional structural diagram of the present invention; Figure 2 This is an assembly diagram showing the cooperation between the sealing component and the column in this utility model; Figure 3 This is a three-dimensional structural diagram of the central column of this utility model; Figure 4 This is a schematic cross-sectional view of the central column of this utility model; Figure 5 This is a three-dimensional structural diagram of the sealing component in this utility model.

[0014] In the diagram: 1. Column; 101. Limiting collar; 102. Fixing collar; 103. Slot; 104. Conical head; 2. Sealing assembly; 201. Sliding collar; 202. Bending part; 203. Cover plate; 204. Plug; 205. Stripe line; 206. Protrusion; 207. Groove; 208. Sealing tube; 3. Filter element. Detailed Implementation

[0015] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0016] like Figure 1-5 As shown, a plasmid extraction column for a cloning kit includes a filter element 3 and a column body 1. The filter element 3 slides within the inner wall of the column body 1. The outer wall of the column body 1 is provided with a limiting collar 101 and a fixing collar 102. Several slots 103 are provided at the top of the limiting collar 101 and the bottom of the fixing collar 102. A sealing assembly 2 is provided on the outer wall of the column body 1. The sealing assembly 2 includes a sliding collar 201, a bent component 202, a cover plate 203, and a sealing tube 208. Several plugs 204 are fixedly installed at both ends of the sliding collar 201. The plugs 204 are capable of... Do not slide into the slot 103 of the limiting collar 101 or the slot 103 of the fixing collar 102. The two ends of the bent part 202 are fixedly connected to the sliding collar 201 and the cover plate 203 respectively. The bottom of the cover plate 203 can fit against the top of the column 1 and the filter element 3. The sealing tube 208 can slide into the inner wall of the column 1 or the filter element 3. The bottom of the column 1 is provided with a conical head 104. The slot 103 and the plug 204 are both pear-shaped. The slot 103, the plug 204 and the sealing tube 208 are all made of rubber. The number of slots 103 and plugs 204 is the same.

[0017] When the sliding collar 201 slides along the outer wall of the column 1, the plug 204 can be inserted into the slot 103 of the limiting collar 101 and the fixing collar 102 respectively, corresponding to the two states of sealing the column 1 and sealing the filter element 3. It is suitable for different operating scenarios such as sample loading, filtering, and cleaning, and will not bend the bending part 202 excessively. This prevents the cover plate 203 from rotating due to the automatic release of pressure after the bending part 202 is over-bent, effectively improving the sealing effect and improving the accuracy of experimental data.

[0018] Both column 1 and filter element 3 are existing technologies and will not be explained here.

[0019] like Figure 5 As shown, in one embodiment, the sliding collar 201 slides with the outer wall of the column 1, the inner wall of the bent part 202 is provided with two protrusions 206, the bottom of the cover plate 203 is provided with stripes 205, and the top is provided with a groove 207. The sealing tube 208 is fixedly installed at the bottom of the cover plate 203. The diameter of the sealing tube 208 is adapted to the inner wall diameter of the column 1 and the filter element 3. The outer wall of the sealing tube 208 is provided with rounded corners.

[0020] The protrusion 206 is used to enhance the structural strength of the bent part, prevent breakage after repeated bending, or assist in positioning the bent part to a specific angle. The stripe 205 can increase the friction between the cover plate 203 and the operator's hand, thus providing an anti-slip effect. The groove 207 facilitates finger gripping and easy opening of the cover plate 203. The top and bottom of the outer wall of the sealing tube 208 are rounded. When the sealing tube 208 is inserted into the inner wall of the column 1 or the filter element 3, the rounded corners can reduce the insertion resistance and prevent scratching the inner wall of the column or the filter element. At the same time, the rounded corner design can make the contact transition between the sealing tube and the inner wall smoother, reduce the liquid residue on the outer wall of the sealing tube, and improve the plasmid extraction and recovery rate.

[0021] Working principle: When it is necessary to open the cover plate 203, the operator clamps the column 1 with one hand and uses one thumb of the other hand to hook onto the stripe line 205. Then, the cover plate 203 is rotated so that the sealing tube 208 is gradually removed from the filter element 3. Then, the experimental material is filled into the filter element 3, and the cover plate 203 is reversed so that the sealing tube 208 is reinserted into the inner wall of the filter element 3, thus resealing the filter element 3. After the filter element 3 is removed, the operator moves the sliding collar 201 vertically downward. The sliding collar 201 slides on the outer wall of the column 1, causing several plugs 204 at the top to move out of the slots 103 at the bottom of the fixed collar 102 until several plugs at the bottom of the sliding collar 201 are inserted into several slots 103 at the top of the limiting collar 101. At this time, by bending the bending piece 202, the cover plate 203 and the sealing tube 208 can be rotated, so that the sealing tube 208 and the cover plate 203 seal the column 1.

[0022] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A plasmid extraction column for a cloning kit, comprising a filter element (3), characterized in that: It also includes a column (1), the filter element (3) slidingly engages with the inner wall of the column (1), the outer wall of the column (1) is provided with a limiting collar (101) and a fixing collar (102), the top of the limiting collar (101) and the bottom of the fixing collar (102) are provided with several slots (103), the outer wall of the column (1) is provided with a sealing assembly (2), the sealing assembly (2) includes a sliding collar (201), a bending part (202), a cover plate (203) and a sealing tube (208), and several plugs (208) are fixedly installed at both ends of the sliding collar (201). 04), several of the plugs (204) can be slidably engaged with the slot (103) of the limiting collar (101) or the slot (103) of the fixing collar (102), the two ends of the bending member (202) are fixedly connected to the sliding collar (201) and the cover plate (203) respectively, the bottom of the cover plate (203) can be attached to the top of the column (1) and the filter element (3), the sealing tube (208) can be slidably engaged with the inner wall of the column (1) or the filter element (3), and the bottom of the column (1) is provided with a conical head (104).

2. The plasmid extraction column for a cloning kit according to claim 1, characterized in that: Both the slot (103) and the plug (204) are pear-shaped, and the slot (103), the plug (204), and the sealing tube (208) are all made of rubber.

3. The plasmid extraction column for a cloning kit according to claim 1, characterized in that: The sliding collar (201) slides in contact with the outer wall of the column (1).

4. The plasmid extraction column for a cloning kit according to claim 1, characterized in that: The inner wall of the bent component (202) is provided with two protrusions (206).

5. The plasmid extraction column for a cloning kit according to claim 1, characterized in that: The bottom of the cover plate (203) is provided with stripes (205), and the top is provided with a groove (207).

6. The plasmid extraction column for a cloning kit according to claim 1, characterized in that: The sealing tube (208) is fixedly installed at the bottom of the cover plate (203), and the diameter of the sealing tube (208) and the inner wall diameter of the column (1) are adapted to each other.

7. The plasmid extraction column for a cloning kit according to claim 1, characterized in that: The outer wall of the sealing tube (208) is provided with rounded corners.

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