Escherichia coli high-density fermentation culture ventilation structure and fermentation tank

Abstract

The utility model discloses a kind of escherichia coli high-density fermentation culture ventilation structure and fermenter, including the ventilation pipe connected with the air inlet of fermenter, the tail end of ventilation pipe extends to the bottom of fermenter, the bottom of fermenter is located in the terminal of ventilation pipe and is provided with aeration structure, wherein, ventilation pipe is by aeration structure and is distributed to the escherichia coli culture solution in fermenter inside oxygen of ventilation pipe.This utility model is by the design of multiple closed circular pipe, air hole spacing 1cm, make oxygen evenly distribute in the bottom of fermenter, increase oxygen and culture medium contact area, improve dissolution efficiency, reduce undissolved oxygen directly discharge, optimize culture solution oxygen concentration distribution, reduce the dependence on high stirring speed, reduce stirring energy consumption, improve fermentation process energy efficiency, more conducive to the use of large-scale fermenter in escherichia coli fermentation culture.

Description

Technical Field

[0001] This utility model relates to a ventilation structure and fermenter for high-density fermentation culture of Escherichia coli. Background Technology

[0002] Escherichia coli is an important host bacterium for biosynthetic technologies, and its fermentation culture, characterized by short growth cycles and high cell density, has been widely used. However, high-density fermentation culture of E. coli requires a large amount of dissolved oxygen in the culture medium to maintain rapid cell proliferation. Low oxygen levels in the culture medium can lead to the accumulation of metabolic products, inhibiting cell proliferation and resulting in slow cell growth, thus reducing the yield of target proteins or nucleic acids.

[0003] Traditional fermenter aeration devices consist of a closed, single-ring circular pipe placed at the bottom of the fermenter, with numerous vents distributed on its lower or upper side. During aeration, the aeration is mixed at the bottom by a stirring paddle, then discharged above the liquid surface in the fermenter, and finally outside the tank. When the stirring speed is insufficient, a large amount of oxygen is easily released directly outside the fermenter without being dissolved in the culture medium. This leads to uneven and insufficient oxygen concentration distribution within the culture medium, which can cause the accumulation of acidic metabolic products and affect cell growth and proliferation.

[0004] Therefore, a high-density fermentation culture aeration structure and fermenter for Escherichia coli are proposed. Utility Model Content

[0005] The purpose of this invention is to provide a high-density fermentation culture ventilation structure and fermenter for Escherichia coli, so as to solve the problem of poor oxygen dissolution effect of traditional fermenters mentioned in the background art.

[0006] To solve the above-mentioned technical problems, this utility model provides the following technical solution: a high-density fermentation culture aeration structure for Escherichia coli, including an aeration pipe connected to the air inlet of the fermenter, the tail end of the aeration pipe extending to the bottom of the fermenter, and an aeration structure provided at the bottom of the fermenter at the end of the aeration pipe. The ventilation pipe distributes oxygen from its interior to the E. coli culture medium in the fermenter via an aeration structure.

[0007] Preferably, the aeration structure includes a coil disposed at the tail end of the air pipe, the upper surface of the coil having a plurality of aeration holes, and the spacing between the aeration holes being 1 cm.

[0008] Preferably, the outer circumferential radius of the coil is smaller than the inner diameter of the fermenter, so that the coil can distribute oxygen to the bottom periphery of the fermenter through the aeration holes.

[0009] Preferably, the vent pipe is installed to the inner wall of the fermenter via fixing buckles, and there are multiple fixing buckles.

[0010] Preferably, the fixing buckle includes two sets of fixing plates, and a fastening plate is provided on the upper part of the fixing plate. A fastening groove for cooperating with the vent pipe is opened between the two sets of fastening plates.

[0011] Preferably, the front end of the fastening groove has an opening for engaging the vent pipe, and the spacing between the openings is smaller than the diameter of the vent pipe.

[0012] Preferably, the fastening plate is provided with a guide plate on the side of the opening, and the guide plate and the opening are arranged at an angle of 45°.

[0013] A fermenter includes a fermenter body for culturing Escherichia coli, the fermenter body being connected to a ventilation structure, an air inlet being provided at the top of the fermenter body, and the air inlet cooperating with a ventilation pipe.

[0014] Preferably, a motor is installed on the upper part of the fermentation tank body, and the output end of the motor is connected to the input end of the stirring rod.

[0015] Compared with the prior art, the beneficial effects of this utility model are: This invention, through its multi-ring closed circular tube design with 1cm spacing between pores, ensures uniform oxygen distribution at the bottom of the fermenter, increases the contact area between oxygen and the culture medium, improves dissolution efficiency, reduces the direct discharge of undissolved oxygen, optimizes the oxygen concentration distribution in the culture medium, reduces dependence on high stirring speed, reduces stirring energy consumption, improves the energy efficiency of the fermentation process, and is more conducive to the use of large fermenters in E. coli fermentation culture.

[0016] The venting pipe is installed on the inner wall of the fermenter through multiple fixing buckles. The fixing buckles consist of two sets of fixing plates and fastening plates. The fastening groove fits tightly with the venting pipe, which can ensure the stability of the venting pipe during fermentation, reduce vibration and displacement, and improve reliability.

[0017] The fastening buckle has an engaging opening at the front of its locking groove and a 45° inclined guide plate on the side for easy positioning of the vent pipe. When maintenance or cleaning is required, the fastening buckle can be quickly removed to take out the vent pipe, improving work efficiency and ensuring the hygiene and performance of the fermenter. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present utility model; Figure 2 This is a cross-sectional structural diagram of an embodiment of the present utility model; Figure 3 This is a schematic diagram of the aeration structure according to an embodiment of the present invention; Figure 4 This is a schematic diagram of the fixing buckle structure according to an embodiment of the present utility model.

[0019] In the diagram: 1. Ventilation pipe; 2. Aeration structure; 21. Coil; 22. Aeration hole; 3. Fixing buckle; 31. Fixing plate; 32. Fastening plate; 33. Fastening groove; 34. Opening; 35. Guide plate; 4. Fermentation tank body; 41. Air inlet; 5. Motor; 51. Stirring rod. Detailed Implementation

[0020] To address the problem of poor oxygen dissolution in traditional fermenters, this invention provides an aeration structure and fermenter for high-density fermentation culture of *E. coli*. The technical solutions of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this invention, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0021] Please see Figure 1-4 This invention provides an aeration structure for high-density fermentation culture of *E. coli*, including an aeration pipe 1 connected to the air inlet 41 of the fermenter. The tail end of the aeration pipe 1 extends to the bottom of the fermenter. An aeration structure 2 is provided at the end of the aeration pipe 1 at the bottom of the fermenter. The aeration structure 2 includes a coil 21 located at the tail end of the aeration pipe 1. The upper surface of the coil 21 has a plurality of aeration holes 22, and the spacing between the aeration holes 22 is 1 cm. The outer circumferential radius of the coil 21 is smaller than the inner diameter of the fermenter, so that the coil 21 can distribute oxygen to the bottom periphery of the fermenter through the aeration holes 22.

[0022] The vent pipe 1 is installed to the inner wall of the fermenter via fixing buckles 3, and there are multiple fixing buckles 3. Each fixing buckle 3 includes two sets of fixing plates 31, with a fastening plate 32 on the upper part of each fixing plate 31. A fastening groove 33 is formed between the two sets of fastening plates 32 to engage with the vent pipe 1. An opening 34 for engaging the vent pipe 1 is formed at the front end of the fastening groove 33, and the spacing between the openings 34 is smaller than the diameter of the vent pipe 1. A guide plate 35 is provided on the side of the fastening plate 32 next to the opening 34, and the guide plate 35 is inclined with the opening 34 at an angle of 45°.

[0023] The ventilation pipe 1 distributes oxygen inside the ventilation pipe 1 evenly to the E. coli culture medium in the fermenter through the aeration structure 2.

[0024] A fermenter includes a fermenter body 4 for culturing Escherichia coli. The fermenter body 4 is connected to a ventilation structure. An air inlet 41 is provided at the upper part of the fermenter body 4, and the air inlet 41 cooperates with a ventilation pipe 1. A motor 5 is provided at the upper part of the fermenter body 4, and the output end of the motor 5 is connected to the input end of a stirring rod 51.

[0025] The working principle of the high-density fermentation culture ventilation structure and fermenter provided by this utility model is as follows: the ventilation pipe 1 is connected to the air inlet 41 of the fermenter. External air or oxygen-enriched gas enters the ventilation pipe 1 through the air inlet 41, and the ventilation pipe 1 transports the gas to the aeration structure 2 at the bottom of the fermenter.

[0026] Multiple aeration holes 22 spaced 1 cm apart are opened on the upper surface of the coil 21 in the aeration structure 2. After the gas enters the coil 21 from the air pipe 1, it is evenly distributed to the bottom of the fermenter through these aeration holes 22.

[0027] The dispersed gas rises in the form of tiny bubbles, making full contact with the E. coli culture medium. As it rises, oxygen continuously dissolves into the culture medium, providing the necessary oxygen for the growth and metabolism of E. coli.

[0028] The motor 5 at the top of the fermenter drives the stirring rod 51 to rotate, stirring the culture medium. Stirring makes the oxygen distribution in the culture medium more uniform, preventing uneven oxygen distribution and local hypoxia.

[0029] Install the two sets of fixing plates 31 of the fixing buckle 3 on the inner wall of the fermentation tank respectively, and use bolts to firmly attach them to the inner wall.

[0030] The vent pipe 1 is introduced from the air inlet 41 at the top of the fermenter and extends gradually downward along the inner wall of the fermenter.

[0031] Align the vent pipe 1 with the guide plate 35 and the opening 34, use the inclined surface of the guide plate 35 to guide the vent pipe 1 into the opening 34, and continue to apply appropriate pressure to make the vent pipe 1 snap into the locking groove 33.

[0032] Repeat the above steps to fix the vent pipe 1 to all the fixing buckles 3 in sequence until the tail end of the vent pipe 1 extends to the bottom of the fermenter and is connected to the aeration structure 2.

[0033] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An aeration structure for high-density fermentation culture of Escherichia coli, characterized in that: It includes an air pipe (1) connected to the air inlet (41) of the fermenter, the tail end of the air pipe (1) extending to the bottom of the fermenter, and an aeration structure (2) is provided at the end of the air pipe (1) at the bottom of the fermenter. The ventilation pipe (1) distributes the oxygen inside the ventilation pipe (1) to the Escherichia coli culture medium in the fermenter through the aeration structure (2).

2. The aeration structure for high-density fermentation culture of Escherichia coli according to claim 1, characterized in that: The aeration structure (2) includes a coil (21) disposed at the end of the air pipe (1), and a plurality of aeration holes (22) are provided on the upper surface of the coil (21), and the spacing between the aeration holes (22) is 1 cm.

3. The aeration structure for high-density fermentation culture of *E. coli* according to claim 2, characterized in that: The outer circumferential radius of the coil (21) is smaller than the inner diameter of the fermenter, so that the coil (21) can distribute oxygen to the bottom circumference of the fermenter through the aeration holes (22).

4. The aeration structure for high-density fermentation culture of *E. coli* according to claim 3, characterized in that: The ventilation pipe (1) is installed on the inner wall of the fermenter through fixing buckles (3), and there are multiple fixing buckles (3).

5. The aeration structure for high-density fermentation culture of *E. coli* according to claim 4, characterized in that: The fixing buckle (3) includes two sets of fixing plates (31), and a fastening plate (32) is provided on the upper part of the fixing plate (31). A fastening groove (33) that cooperates with the vent pipe (1) is provided between the two sets of fastening plates (32).

6. The aeration structure for high-density fermentation culture of *E. coli* according to claim 5, characterized in that: The front end of the fastening groove (33) is provided with an opening (34) for engaging the vent pipe (1), and the spacing of the openings (34) is smaller than the diameter of the vent pipe (1).

7. The aeration structure for high-density fermentation culture of *E. coli* according to claim 6, characterized in that: The fastening plate (32) is provided with a guide plate (35) on the side of the opening (34), and the guide plate (35) and the opening (34) are arranged at an angle of 45°.

8. A fermentation tank, characterized in that: The fermenter includes a fermenter body (4) for culturing Escherichia coli, the fermenter body (4) being connected to a ventilation structure, the ventilation structure being the ventilation structure described in claim 1, the upper part of the fermenter body (4) having an air inlet (41), and the air inlet (41) cooperating with the ventilation pipe (1).

9. A fermenter according to claim 8, characterized in that: The upper part of the fermentation tank body (4) is equipped with a motor (5), and the output end of the motor (5) is connected to the input end of the stirring rod (51).

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