In-situ reaction culture device for microalgae

Abstract

The utility model relates to the field of microalgae culture biological technology and disclose a kind of microalgae in situ reaction culture device, including incubator body, incubator body is equipped with the culture platform of rotation by support shaft drive, and culture platform is equipped with clamping fixture;Incubator body top is fixed with the liquid supply end cover of the liquid supply cavity being equipped with in communication with infusion tube, support shaft upper portion is rotatably connected with liquid supply end cover, and inside is provided with infusion channel in communication with liquid supply cavity, outside is equipped with fractionating pipe in communication with infusion channel and with clamping fixture one-to-one correspondence;The device improves culture environment uniformity by culture container rotation, realizes continuous liquid supply under rotating state, without stopping machine opening box can complete in situ reaction, reduce environmental fluctuation and pollution risk, significantly improve the reliability and repeatability of experimental result, solve the problem that environment uniformity is poor in prior art, and continuous liquid supply cannot be realized under the continuous rotating state of culture container.

Description

Technical Field

[0001] This utility model relates to the field of microalgae culture biotechnology, specifically to a microalgae in-situ reaction culture device. Background Technology

[0002] Microalgae have wide applications in bioenergy, environmental remediation, heavy metal adsorption, and metabolic regulation. The stability and uniformity of laboratory culture conditions are prerequisites for ensuring accurate and reliable experimental results. Currently, laboratories commonly use constant-temperature, light-controlled incubators combined with conical flasks for static or shaker culture. However, due to uneven distribution of light and temperature fields within the incubator, environmental conditions vary between culture containers in different locations. Furthermore, static culture is prone to problems such as algal cell sedimentation and uneven mixing, ultimately leading to significant differences in culture results between parallel samples, affecting the accuracy and reproducibility of the experiment.

[0003] To improve the environmental uniformity and increase cultivation efficiency during microalgae cultivation, various rotating microalgae cultivation devices have been developed in the prior art. For example, Chinese Patent CN107446801B discloses a rotating microalgae cultivation device with light reflection, which significantly improves the uniformity of light exposure during microalgae cultivation by rotating a double-layered spherical cultivation shell and using an internal mirror reflection structure. Chinese Patent CN105733930A discloses a rotating photobioreactor for large-scale microalgae cultivation, which rotates the cultivation carrier to allow attached algal cells to alternately enter the liquid and gas phases, increasing the contact opportunities between algal cells and the light and gaseous environment. These technical solutions mainly focus on solving the problem of uniform light conditions during the cultivation stage, thus improving the stability and efficiency of microalgae cultivation to a certain extent.

[0004] However, current technologies cannot achieve continuous and stable liquid addition while the culture vessel is constantly rotating. In subsequent experiments such as heavy metal stress and nutrient regulation, manual opening of the container for drug addition or interruption of the culture connection tubing is still required. This is not only cumbersome but also causes fluctuations in the culture environment and the risk of contamination by other microorganisms. Furthermore, conventional infusion tubing is prone to tangling and pulling during rotation, severely limiting the combined application of rotational culture with automated liquid addition and in-situ reaction technologies.

[0005] Therefore, there is an urgent need to design an integrated microalgae cultivation device that can achieve continuous liquid supply while the culture container is continuously rotating, and can complete the cultivation and in-situ reaction process in the same closed culture environment. Utility Model Content

[0006] The purpose of this invention is to overcome the shortcomings of the prior art and provide a microalgae in-situ reaction culture device. By rotating the culture platform, the culture containers are dynamically rotated during the culture process, so that each culture container periodically passes through different areas in the culture chamber, thereby reducing the long-term impact of local environmental differences on the microalgae culture results. At the same time, continuous liquid supply can be achieved while the culture containers are rotating continuously, so as to complete the culture and in-situ reaction process in the same closed culture environment.

[0007] To achieve the above objectives, the technical solution adopted by this utility model is as follows: A microalgae in-situ reaction culture device includes a culture chamber with a culture cavity inside. A culture platform is horizontally arranged inside the culture cavity. Multiple clamping fixtures for clamping culture containers are arranged on the culture platform. The culture platform is connected to the culture chamber through a support shaft. A liquid supply end cap is fixedly installed on the top of the culture chamber. The liquid supply end cap has a liquid supply cavity inside that communicates with a liquid delivery pipe. The upper part of the support shaft passes through the culture chamber and is rotatably connected to the liquid supply end cap. The inside of the support shaft is provided with a liquid infusion channel communicating with the liquid supply chamber. The outside of the support shaft is provided with a liquid distribution pipe corresponding to the position and number of the clamping fixture. The lower part of the support shaft is fixedly connected to the culture platform, and the culture platform can rotate relative to the culture chamber around the axis of the support shaft. One end of the dispensing tube is connected to the infusion channel, and the other end extends into the culture container on the corresponding clamping fixture, and the dispensing tube moves synchronously with the support shaft.

[0008] Optionally, a plurality of the clamping fixtures are arranged in a circumferential array around the axis of the support shaft, and the clamping fixtures are located at the edge of the culture platform.

[0009] Optionally, the clamping fixture includes a fixture body connected to the culture platform, the top of the fixture body having a slot for fixing the culture container, and the slot being located above the culture platform.

[0010] Optionally, the fixture body is rotatably connected to the culture platform, the bottom of the fixture body extends to the bottom of the culture platform and is fixedly connected to a driven gear, and an internal gear ring that meshes with the driven gear is provided below the culture platform, and the internal gear ring is fixedly connected to the culture chamber.

[0011] Optionally, the inner side of the slot is provided with an elastic clamping structure for holding the culture container, and the bottom surface of the slot is provided with an anti-slip pad.

[0012] Optionally, the elastic clamping structure includes multiple arc-shaped clamps adapted to the outer contour of the culture container. The arc-shaped clamps are connected to the inner wall of the slot by springs, and the multiple arc-shaped clamps are arranged in a circumferential array around the axis of the slot.

[0013] Optionally, a drive motor and a reduction gearbox are provided at the bottom of the culture chamber, the output end of the drive motor is connected to the input end of the reduction gearbox, and the lower end of the support shaft passes through the culture platform and is connected to the output end of the reduction gearbox.

[0014] Optionally, the incubator is provided with multiple LED light strips, which are distributed at intervals along the vertical direction, and each LED light strip is circumferentially attached to the inner wall of the incubator.

[0015] Compared with the prior art, the beneficial effects of this utility model are as follows: (1) In this utility model, the rotating culture platform can drive the culture container on the clamping fixture to rotate in the culture chamber. Through the dynamic rotation of the culture container during the culture process, each culture container can periodically pass through different areas, thereby reducing the long-term impact of local environmental differences on the microalgae culture results and improving the culture consistency. At the same time, the liquid can be injected into the culture container in sequence along the infusion pipe, the liquid supply chamber, the infusion channel and the liquid distribution pipe. The culture container, the liquid distribution pipe and the support shaft can rotate synchronously, and the support shaft can rotate relative to the liquid supply end cap, so as to realize continuous liquid supply in the continuous rotation state of the culture container, thereby realizing the culture and in-situ reaction process in the same closed culture environment, solving the problem that the traditional drug addition process requires stopping the machine or opening the culture box. Moreover, the drug addition process can be completed in the continuous culture state, which can reduce the fluctuation of the culture environment and reduce the risk of pollution. (2) In this utility model, during the rotation of the culture platform, the clamping fixture can not only revolve around the axis of the support shaft, but also rotate around its own axis through the meshing of the driven gear and the internal gear ring, so that different sides of the culture container can receive light in turn, thereby improving the effect of uneven local light on the culture container. (3) In this utility model, the culture container is embedded in the slot and clamped and fixed by the elastic clamping structure in the slot. Its arc-shaped clamping plate can extend and retract along the radial direction of the slot, so as to adapt to culture containers of different sizes and specifications, greatly improving the compatibility of the device. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the internal structure of the microalgae in-situ reaction culture device in this embodiment of the present invention; Figure 2 This is a schematic diagram of the structure of the in-situ reaction culture device for microalgae in this embodiment of the present invention; Figure 3This is a schematic diagram showing the positional structure of the driven gear and the internal gear ring in an embodiment of this utility model; Figure 4 This is a schematic diagram showing the position and structure of the circulating fan and the air supply channel in an embodiment of this utility model; Figure 5 This is a schematic diagram of the clamping fixture in an embodiment of this utility model; Among them, 1. incubator body; 2. incubation platform; 3. clamping fixture; 301. fixture body; 302. slot; 303. arc-shaped clamping plate; 304. spring; 305. anti-slip pad; 4. Liquid supply end cap; 401. Liquid supply chamber; 5. Support shaft; 501. Infusion channel; 6. Infusion tube; 7. Dispensing tube; 8. Driven gear; 9. Internal gear ring; 10. Drive motor; 11. Gearbox; 12. LED light strip; 13. Circulating fan; 14. Air supply channel; 15. Baffle plate; 16. Storage bottle; 17. Culture container; 18. Peristaltic pump. Detailed Implementation

[0017] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. These drawings are simplified schematic diagrams, which are only used to illustrate the basic structure of the present invention in a schematic manner, and therefore only show the components related to the present invention.

[0018] Example 1, as Figures 1-5 As shown, a microalgae in-situ reaction culture device includes a culture chamber 1, a culture platform 2, a clamping fixture 3, a liquid supply end cap 4, a support shaft 5, a dispensing pipe 7, and an LED light strip 12. The culture chamber 1 has a culture cavity inside, the culture platform 2 is horizontally arranged in the culture cavity, the clamping fixture 3 is arranged on the culture platform 2 and used to clamp the culture container 17, the liquid supply end cap 4 is fixedly installed on the top of the culture chamber 1, the support shaft 5 is vertically arranged in the culture cavity, and the culture platform 2 is connected to the culture chamber 1 through the support shaft 5, and the culture platform 2 can rotate relative to the culture chamber 1 around the axis of the support shaft 5. The liquid supply end cap 4 has a liquid supply cavity 401 inside that communicates with the liquid delivery pipe 6.

[0019] The upper part of the support shaft 5 passes through the culture chamber 1 and is rotatably connected to the liquid supply end cap 4. An infusion channel 501 communicating with the liquid supply chamber 401 is provided inside the support shaft 5. A dispensing pipe 7, corresponding to the position and number of clamping fixtures 3, is provided on the outer side of the support shaft 5. The lower part of the support shaft 5 is fixedly connected to the culture platform 2, and the culture platform 2 can rotate relative to the culture chamber 1 around the axis of the support shaft 5. One end of the dispensing pipe 7 is connected to the infusion channel 501, and the other end extends into the culture container 17 on the corresponding clamping fixture 3. The dispensing pipe 7 moves synchronously with the support shaft 5, i.e., the dispensing pipe 7 is fixedly installed on the outer circumferential surface of the support shaft 5. When the culture platform 2 rotates continuously, the liquid supply end cap 4 remains stationary as a fixed liquid supply end, while the support shaft 5 rotates synchronously with the culture platform 2 as a rotating connection end. The drug solution is continuously delivered to each culture container 17 through the infusion channel 501, thereby achieving in-situ drug delivery while the culture container 17 is continuously rotating, and avoiding entanglement of the infusion pipe 6.

[0020] To ensure sealing, a sealing groove is formed on the inner wall of the mating hole between the liquid supply end cap 4 and the support shaft 5. An O-ring or Y-ring resistant to chemical corrosion can be embedded in this groove, forming an interference fit with the smooth outer surface of the support shaft 5. This reliably achieves radial sealing during rotation, preventing leakage of the chemical solution. The aforementioned sealing structures are standard and universal configurations in the field of fluid rotary conveying, with mature industry standards and selection specifications, allowing for direct selection and application. Further details are omitted here.

[0021] Specifically, LED light strip 12 is installed inside the culture chamber 1 as a light source, and the culture platform 2 is a circular tray located in the central area of ​​the culture chamber. A drive motor 10 for driving its own rotation is installed below it. The rotating culture platform 2 can drive the culture container 17 on the clamping fixture 3 to rotate in the culture chamber. Through the dynamic rotation of the culture container 17 during the culture process, each culture container 17 periodically passes through different areas, thereby reducing the long-term impact of local environmental differences on the microalgae culture results and improving the consistency of culture.

[0022] The liquid to be injected can be sequentially injected into the culture container 17 through the infusion tube 6, the supply chamber 401, the infusion channel 501, and the dispensing tube 7. The culture container 17, the dispensing tube 7, and the support shaft 5 can rotate synchronously, and the support shaft 5 can also rotate relative to the supply end cap 4, so as to achieve continuous liquid supply while the culture container 17 is continuously rotating. This allows the culture and in-situ reaction process to be completed in the same closed culture environment, solving the problem that the traditional drug addition process requires stopping the machine or opening the culture chamber 1. Moreover, the drug addition process can be completed while the culture is in progress, which can reduce the fluctuation of the culture environment and reduce the risk of contamination.

[0023] As described above, the incubator 1 is a closed structure with a double-layered insulated shell, consisting of an outer metal shell, an inner corrosion-resistant liner, and a middle insulation layer. The inner liner is made of stainless steel. The incubator 1 contains a culture chamber for placing the culture platform 2. An observation window, made of transparent tempered glass, is hinged to the front side for observing the culture status. A sealing strip is installed between the observation window and the incubator 1 to reduce heat exchange and maintain a stable culture environment.

[0024] Multiple LED light strips 12 are provided and fixedly installed on the inner wall of the incubator 1. These LED light strips 12 are distributed vertically at intervals, and each LED light strip 12 is circumferentially attached to the inner wall of the incubator. That is, multiple layers of LED light strips 12 are arranged vertically at intervals and connected to the inner wall of the incubator 1 via fixed brackets. A diffuse reflector is provided on the outer side of the LED light strips 12 to reduce localized intense light and improve illumination uniformity. The LED light strips 12, as light sources, include red LEDs, blue LEDs, and white LEDs, where the blue light wavelength is approximately 450nm and the red light wavelength is approximately 660nm. The illumination intensity can be adjusted within the range of 3000–8000 lux.

[0025] Furthermore, multiple clamping fixtures 3 are arranged in a circular array around the axis of the support shaft 5, and the clamping fixtures 3 are located at the edge of the culture platform 2, which facilitates the assembly and disassembly of the culture container 17 (such as a conical flask) and ensures that the culture container 17 passes through the same culture area during the rotation around the axis of the support shaft 5.

[0026] In addition, the upper part of the support shaft 5 is connected to the box body through a bearing, which can not only achieve rotation around its own axis, but also achieve axial connection between the culture box body 1 and the culture platform 2 through the axial positioning of the bearing. The lower part of the support shaft 5 is fixedly connected to the culture platform 2, thereby realizing the synchronous rotational movement of the culture platform 2 and the support shaft 5.

[0027] Example 2: Based on Example 1, this utility model also proposes a specific structure for the clamping fixture 3.

[0028] like Figure 5 As shown, the clamping fixture 3 includes a fixture body 301, an elastic clamping structure, and an anti-slip pad 305. The fixture body 301 is connected to the culture platform 2. A slot 302 is provided on the top of the fixture body 301. The slot 302 is located above the culture platform 2 to facilitate the fixing of the culture container 17. The anti-slip pad 305 is embedded in the bottom surface of the slot 302. The elastic clamping structure is installed inside the slot 302. Through the elastic clamping structure and the anti-slip pad 305 in the slot 302, the culture container 17 can be stably fixed after being inserted into the slot 302.

[0029] The elastic clamping structure is used to clamp the culture container 17. It includes multiple arc-shaped clamping plates 303 that are adapted to the outer contour of the culture container 17. The arc-shaped clamping plates 303 are connected to the inner wall of the slot 302 by springs 304, and the multiple arc-shaped clamping plates 303 are arranged in a circumferential array around the axis of the slot 302. The culture container 17 is inserted into the slot 302 and clamped and fixed by the elastic clamping structure in the slot 302. The arc-shaped clamping plates 303 can extend and retract along the radial direction of the slot 302, thereby adapting to culture containers 17 of different sizes and specifications, greatly improving the compatibility of the device.

[0030] In Example 3, based on Example 2, this utility model also proposes another installation structure for the clamping fixture 3, so that the clamping fixture 3 can not only revolve around the axis of the support shaft 5, but also rotate around its own axis.

[0031] like Figure 3 and Figure 5 As shown, the fixture body 301 is rotatably connected to the culture platform 2. The bottom of the fixture body 301 extends to the bottom of the culture platform 2 and is fixedly connected to the driven gear 8. An internal gear ring 9 that meshes with the driven gear 8 is provided on the bottom of the culture platform 2, and the internal gear ring 9 is fixedly connected to the culture box 1.

[0032] Specifically, the fixture body 301 is rotatably connected to the culture platform 2 via bearings, the driven gear 8 is fixed at the bottom of the fixture body 301, and the two can rotate synchronously. The internal gear ring 9 is fixed inside the culture box 1 and meshes with the driven gear 8. As the culture platform 2 drives the clamping fixture 3 to rotate around the axis of the support shaft 5, due to the meshing relationship, the driven gear 8 meshes with the internal gear ring 9 and rotates, thereby driving the fixture body 301 to rotate around its own axis, realizing its self-rotation function.

[0033] Therefore, during the rotation of the culture platform 2, the clamping fixture 3 can not only revolve around the axis of the support shaft 5, but also rotate around its own axis through the meshing of the driven gear 8 and the internal gear ring 9, so that different sides of the culture container 17 can receive light in turn, thereby improving the effect of uneven local light exposure of the culture container 17.

[0034] Example 4: Based on the above examples, this utility model also proposes a specific installation structure for the drive motor 10.

[0035] like Figures 1-3 As shown, the drive motor 10 is fixedly installed at the bottom of the culture chamber, and a reduction gearbox 11 is also provided to ensure that the torque output by the drive motor 10 can drive the culture platform 2 to rotate stably; the output end of the drive motor 10 is connected to the input end of the reduction gearbox 11, and the lower end of the support shaft 5 passes through the culture platform 2 and is connected to the output end of the reduction gearbox 11.

[0036] As described above, the culture platform 2 is rotatably connected to the culture chamber 1 via the support shaft 5, and the drive motor 10 is located at the bottom of the culture chamber, below the culture platform 2, to drive the culture platform 2 to achieve the rotation function. This structural layout makes the distribution of the culture platform 2, the support shaft 5 and the drive motor 10 more reasonable, while ensuring that the device can operate stably.

[0037] Example 5: An in-situ drug delivery structure is provided on the outside of the incubator 1, which includes a storage bottle 16, a peristaltic pump 18, an infusion tube 6, and a solenoid valve.

[0038] like Figures 1-2 As shown, the storage bottle 16 is used to store reaction reagents, culture additives, etc. The storage bottle 16 is connected to the supply chamber 401 in the aforementioned supply end cap 4 through the infusion tube 6. A peristaltic pump 18 is installed on the infusion tube 6. The input end of the peristaltic pump 18 is connected to the storage bottle 16 through the infusion tube 6, and the output end of the peristaltic pump 18 is connected to the supply chamber 401 through the infusion tube 6. The supply chamber 401 is then connected to the corresponding culture container 17 through the infusion channel 501 and the dispensing tube 7. Each infusion tube 6 can be equipped with an independent solenoid valve to realize the opening and closing of the infusion tube 6 and the flow rate adjustment.

[0039] The infusion channel 501 is located at the center of the support shaft 5 along its axial direction. The dispensing tubes 7 correspond one-to-one with the clamping fixtures 3, arranged in a standard circular array around the support shaft 5. The diameter, flow channel length, and radial position and axial height of the inlet of all dispensing tubes 7 within the infusion channel 501 are completely consistent. During operation, the support shaft 5 drives the dispensing tubes 7 to rotate synchronously and uniformly with the culture platform 2. The liquid in the infusion channel 501 flows radially outward, driven by centrifugal force and supplemented by gravity. Because the inlet of each dispensing tube 7 is in a completely symmetrical centrifugal force field and flow channel resistance environment, the liquid is evenly distributed to each dispensing tube 7 according to the number of branches. The flow rate deviation of a single branch is determined only by the machining accuracy and is far below the allowable error range for microalgae culture experiments.

[0040] Example 6, as Figure 4 As shown, an air duct is also provided inside the culture chamber 1, located at the bottom and side wall of the culture chamber. It includes an air supply channel 14, a return air channel, and a guide plate 15. The circulating fan 13 is connected to the air duct and installed at the bottom inside the culture chamber 1. When the circulating fan 13 is working, air enters the bottom of the culture chamber through the air supply channel 14. Then, under the action of the guide plate 15, the air flows upward along the inner side wall of the culture chamber 1. After passing through the culture area, the air returns to the circulating fan 13 through the top return air channel, thus forming a closed-loop circulating airflow.

[0041] Controlling the temperature of the culture environment is a well-established technology, so it will not be elaborated on here.

[0042] The workflow of this invention includes a cultivation stage and a reaction stage: Cultivation stage: The LED light strip 12, circulating fan 13 and drive motor 10 are started. The cultivation platform 2 rotates slowly. Each cultivation container 17 passes through different light and temperature areas periodically, thereby reducing the impact of local environmental differences in the cultivation chamber on the cultivation results. At the same time, the cultivation container 17 rotates on its own axis while revolving with the cultivation platform 2, so as to improve the uniformity of light exposure on different sides of the cultivation container 17. Reaction stage: Start the peristaltic pump 18 and solenoid valve. The reaction solution is injected into the culture container 17 through the storage bottle 16, infusion tube 6, supply chamber 401, infusion channel 501 and dispensing tube 7 in sequence, realizing in-situ quantitative drug addition. The entire process does not require opening the incubator 1 or stopping the operation of the culture platform 2.

[0043] In summary, the microalgae in-situ reaction culture device proposed in this utility model improves the uniformity of the culture environment by rotating the culture container 17, and realizes continuous liquid supply in the rotating state. The in-situ reaction can be completed without stopping the machine and opening the box, reducing environmental fluctuations and pollution risks, and significantly improving the reliability and reproducibility of experimental results. It solves the problems of poor environmental uniformity and inability to achieve continuous liquid supply in the culture container 17 under continuous rotation in the prior art.

[0044] The embodiments of the present invention have been described above with reference to the accompanying drawings. However, the present invention is not limited to the specific embodiments described above. The specific embodiments described above are merely illustrative and not restrictive. Those skilled in the art can make many improvements and modifications under the guidance of the present invention without departing from the spirit and scope of the claims. These improvements and modifications should also be considered within the scope of protection of the present invention.

[0045] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", and "outer" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0046] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0047] Furthermore, the terms "first," "second," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Therefore, a feature defined with "first," "second," etc., may explicitly or implicitly include one or more of that feature. In the description of this utility model, unless otherwise stated, "a plurality of" means two or more.

Claims

1. A microalgae in-situ reaction culture device, characterized in that: The invention includes a culture chamber with an internal culture cavity, a culture platform horizontally arranged inside the culture cavity, a plurality of clamping fixtures for clamping culture containers arranged on the culture platform, and the culture platform connected to the culture chamber via a support shaft. A liquid supply end cap is fixedly installed on the top of the culture chamber, and a liquid supply cavity communicating with a liquid infusion tube is opened inside the liquid supply end cap. The upper part of the support shaft passes through the culture chamber and is rotatably connected to the liquid supply end cap. The inside of the support shaft is provided with a liquid infusion channel communicating with the liquid supply chamber. The outside of the support shaft is provided with a liquid distribution pipe corresponding to the position and number of the clamping fixture. The lower part of the support shaft is fixedly connected to the culture platform, and the culture platform can rotate relative to the culture chamber around the axis of the support shaft. One end of the dispensing tube is connected to the infusion channel, and the other end extends into the culture container on the corresponding clamping fixture, and the dispensing tube moves synchronously with the support shaft.

2. The microalgae in-situ reaction culture device according to claim 1, characterized in that: The clamping fixtures are arranged in a circular array around the axis of the support shaft, and the clamping fixtures are located at the edge of the culture platform.

3. The microalgae in-situ reaction culture device according to claim 2, characterized in that: The clamping fixture includes a fixture body connected to the culture platform. The top of the fixture body has a slot for fixing the culture container, and the slot is located above the culture platform.

4. The microalgae in-situ reaction culture device according to claim 3, characterized in that: The fixture body is rotatably connected to the culture platform. The bottom of the fixture body extends to the bottom of the culture platform and is fixedly connected to a driven gear. An internal gear ring that meshes with the driven gear is provided below the culture platform, and the internal gear ring is fixedly connected to the culture chamber.

5. The microalgae in-situ reaction culture device according to claim 3, characterized in that: The inner side of the slot is provided with an elastic clamping structure for holding the culture container, and the bottom surface of the slot is provided with an anti-slip pad.

6. The microalgae in-situ reaction culture device according to claim 5, characterized in that: The elastic clamping structure includes multiple arc-shaped clamps that are adapted to the outer contour of the culture container. The arc-shaped clamps are connected to the inner wall of the slot by springs, and the multiple arc-shaped clamps are arranged in a circumferential array around the axis of the slot.

7. The microalgae in-situ reaction culture device according to claim 1, characterized in that: The bottom of the culture chamber is equipped with a drive motor and a reduction gearbox. The output end of the drive motor is connected to the input end of the reduction gearbox, and the lower end of the support shaft passes through the culture platform and is connected to the output end of the reduction gearbox.

8. The microalgae in-situ reaction culture device according to any one of claims 1-7, characterized in that: The incubator is equipped with multiple LED light strips, which are distributed at intervals along the vertical direction, and each LED light strip is circumferentially attached to the inner wall of the incubator.

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