A mesenchymal stem cell resuscitation device

Abstract

The utility model relates to the technical field of cell biology, especially to a kind of mesenchymal stem cell's recovery device, it is stored to mesenchymal stem cell by lifting device and holding device cooperation, the recovery activity of mesenchymal stem cell in holding device is detected by detection device, the temperature monitoring of water in the inside of storage device is carried out by monitoring device, the water in the inside of storage device is heated by heating device, the water in the inside of storage device is agitated by agitating device;Including storage device;Still including lifting device, holding device, detection device, monitoring device, heating device and agitating device, lifting device, detection device, monitoring device, heating device and agitating device are all installed on storage device, and holding device is installed on lifting device.

Description

Technical Field

[0001] This invention relates to the technical field of cell biology, and in particular to a device for the resuscitation of mesenchymal stem cells. Background Technology

[0002] Stem cells are stored in liquid nitrogen to maintain a dormant state. When ready for use, they need to be revived to regain their activity. Revival involves removing the stem cells from the liquid nitrogen tank and heating them to 37°C. This means rapidly passing them through the temperature range of -5°C to 0°C, where cells are most vulnerable, to prevent damage from ice crystal recrystallization. To meet the conditions for stem cell revival, a revival device for mesenchymal stem cells is required.

[0003] For example, in a prior art represented by application number CN202322441421.5, the main structure includes a water bath and a rectangular frame. A first servo motor is installed on one side of the water bath, and a first support rod is installed at the output end of the first servo motor. A second support rod is rotatably connected to the end of the first support rod away from the first servo motor via a shaft. This mesenchymal stem cell resuscitation device, through the arrangement of the rectangular frame, the first servo motor, the first support rod, the second support rod, the cooperating block, the positioning slider, and the strip plate, facilitates the shaking of cryopreservation tubes, resulting in more uniform heating of the cryopreservation tubes. The cooperation of the first and second support rods allows the rectangular frame to easily drive the cryopreservation tube holder to shake back and forth, making the cryopreservation tubes heat more evenly, accelerating the resuscitation of stem cells, and achieving the goal of improving practicality.

[0004] During use, it was found that existing mesenchymal stem cell resuscitation devices are inconvenient to maintain a suitable water temperature, resulting in poor resuscitation effects. Therefore, there is an urgent need for a new mesenchymal stem cell resuscitation device. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a mesenchymal stem cell resuscitation device that uses a lifting device and a holding device to hold mesenchymal stem cells, a detection device to detect the resuscitation vitality of the mesenchymal stem cells in the holding device, a monitoring device to monitor the temperature of the water inside the storage device, a heating device to heat the water inside the storage device, and a stirring device to agitate the water inside the storage device.

[0006] This utility model discloses a resuscitation device for mesenchymal stem cells, comprising a storage device; it also includes a lifting device, a holding device, a detection device, a monitoring device, a heating device, and a stirring device. The lifting device, detection device, monitoring device, heating device, and stirring device are all installed on the storage device, and the holding device is installed on the lifting device. The lifting device performs lifting and lowering, the holding device facilitates holding, the detection device detects resuscitation viability, the monitoring device monitors temperature, the heating device heats the water, and the stirring device stirs. The lifting device and the holding device work together to hold the mesenchymal stem cells, the detection device detects the resuscitation viability of the mesenchymal stem cells in the holding device, the monitoring device monitors the temperature of the water inside the storage device, the heating device heats the water inside the storage device, and the stirring device stirs the water inside the storage device.

[0007] Preferably, the storage device includes a reaction pool and a drain pipe. The reaction pool is installed in the working area, and the drain pipe is connected to the side of the reaction pool. A valve is provided on the drain pipe. The reaction pool provides a place for the resuscitation of mesenchymal stem cells. By opening the valve, the water inside the reaction pool is discharged through the output end of the drain pipe.

[0008] Preferably, the lifting device includes a dual-axis cylinder, a lifting bracket, a guide shaft, and a guide sleeve. The dual-axis cylinder is installed at the top of the reaction tank and has two sets of output shafts. The lifting bracket is installed at the bottom of the two sets of output shafts of the dual-axis cylinder and has multiple sets of through holes. The guide shaft is installed on the reaction tank, and the guide sleeve is installed on the lifting bracket and fitted onto the guide shaft. By starting the dual-axis cylinder, the two sets of output shafts of the dual-axis cylinder are extended, and the lifting bracket is directionally lowered on the guide shaft via the guide sleeve.

[0009] Preferably, the holding device includes multiple sets of test tubes, all of which pass through the through holes of the lifting support; the test tubes facilitate the holding of mesenchymal stem cells.

[0010] Preferably, the detection device includes a resuscitation rate detection instrument, which is installed on the reaction tank; the resuscitation rate detection instrument is activated to detect the viability of mesenchymal stem cells in the test tube.

[0011] Preferably, the monitoring device includes a temperature monitoring sensor, which is installed on the side of the reaction tank; the temperature of the water inside the reaction tank is monitored by the temperature monitoring sensor.

[0012] Preferably, the heating device includes a first pipe, a power pump, a second pipe, a heater, and a third pipe. The first pipe is connected to and installed at the side end of the reaction tank. The power pump and the heater are both installed at the side end of the reaction tank. The input end of the power pump is connected to the output end of the first pipe, the output end of the power pump is connected to the input end of the second pipe, the output end of the second pipe is connected to the input end of the heater, the output end of the heater is connected to the input end of the third pipe, and the third pipe is connected to and installed at the side end of the reaction tank. By starting the power pump and the heater, water inside the reaction tank enters through the input end of the first pipe. The water flows into the heater through the second pipe and is heated by the heater. The heated water flows back into the reaction tank through the third pipe.

[0013] Preferably, the agitation device includes a motor and a motor shaft. The motor is installed on the side of the reaction tank, and the output end of the motor is rotatably connected to the input end of the motor shaft. Multiple sets of stirring blades are provided on the motor shaft. When the motor is started, the motor shaft and stirring blades are driven to rotate, thereby agitating the water inside the reaction tank.

[0014] Compared with the prior art, the beneficial effects of this utility model are as follows: mesenchymal stem cells are placed in the container by means of a lifting device and a holding device; the resuscitation vitality of the mesenchymal stem cells in the holding device is detected by a detection device; the temperature of the water inside the storage device is monitored by a monitoring device; the water inside the storage device is heated by a heating device; and the water inside the storage device is stirred by a stirring device. Attached Figure Description

[0015] Figure 1 This is an isometric drawing of the structure of this utility model;

[0016] Figure 2 yes Figure 1 Structural isometric drawing of the storage device and stirring device in this utility model;

[0017] Figure 3 yes Figure 1 Structural isometric drawing of the storage device and monitoring device in this utility model;

[0018] Figure 4 yes Figure 1 Enlarged structural cross-sectional view of the storage device and lifting device in this utility model.

[0019] The attached diagram is labeled as follows: 01, storage device; 11, reaction tank; 12, drain pipe; 13, valve; 02, lifting device; 21, dual-shaft cylinder; 22, lifting support; 23, guide shaft; 24, guide sleeve; 03, holding device; 31, test tube; 04, detection device; 41, recovery rate detection instrument; 05, monitoring device; 51, temperature monitoring sensor; 06, heating device; 61, first pipeline; 62, power pump; 63, second pipeline; 64, heater; 65, third pipeline; 07, stirring device; 71, motor; 72, motor shaft; 73, stirring blade. Detailed Implementation

[0020] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. This utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to make the disclosure of this utility model more thorough and complete.

[0021] Example 1

[0022] A resuscitation device for mesenchymal stem cells includes a storage device 01; characterized in that it further includes a lifting device 02, a holding device 03, a detection device 04, a monitoring device 05, a heating device 06, and a stirring device 07, wherein the lifting device 02, the detection device 04, the monitoring device 05, the heating device 06, and the stirring device 07 are all installed on the storage device 01, and the holding device 03 is installed on the lifting device 02;

[0023] Lifting device 02 lifts and lowers the water, holding device 03 facilitates holding the water, detection device 04 detects the resuscitation vitality, monitoring device 05 monitors the temperature, heating device 06 heats the water, and stirring device 07 stirs the water.

[0024] The storage device 01 includes a reaction tank 11 and a drain pipe 12. The reaction tank 11 is installed in the working area, and the drain pipe 12 is connected to the side end of the reaction tank 11. A valve 13 is provided on the drain pipe 12.

[0025] The lifting device 02 includes a dual-axis cylinder 21, a lifting bracket 22, a guide shaft 23, and a guide sleeve 24. The dual-axis cylinder 21 is installed at the top of the reaction tank 11 and has two sets of output shafts. The lifting bracket 22 is installed at the bottom of the two sets of output shafts of the dual-axis cylinder 21 and has multiple sets of through holes. The guide shaft 23 is installed on the reaction tank 11, and the guide sleeve 24 is installed on the lifting bracket 22 and is fitted onto the guide shaft 23.

[0026] The holding device 03 includes multiple sets of test tubes 31, all of which pass through the through holes of the lifting bracket 22.

[0027] The detection device 04 includes a resuscitation rate detection instrument 41, which is installed on the reaction tank 11;

[0028] The monitoring device 05 includes a temperature monitoring sensor 51, which is installed on the side of the reaction tank 11.

[0029] Lifting device 02 lifts the object up and down, holding device 03 facilitates holding the object, detection device 04 detects the resuscitation vitality, and monitoring device 05 monitors the temperature.

[0030] Warm water is poured into the reaction tank 11, then mesenchymal stem cells are placed into test tube 31, along with a resuscitation staining agent. Test tube 31 is then placed in the through-hole of the lifting support 22. The dual-axis cylinder 21 is then activated, extending its two output shafts. The lifting support 22 is lowered via guide sleeve 24 on guide shaft 23, directionally lowering test tube 31 into the warm water to resuscitate the mesenchymal stem cells. During the resuscitation process, the temperature of the water inside the reaction tank 11 is monitored by temperature sensor 51. If the temperature is insufficient, warm water is added to raise the resuscitation temperature. After the designated resuscitation time, the dual-axis cylinder 21 is activated, extending its two output shafts. The two sets of output shafts of cylinder 21 retract, causing the lifting support 22 to rise directionally on guide shaft 23 via guide sleeve 24. Then, by activating the resuscitation rate detection instrument 41, the viability of mesenchymal stem cells in test tube 31 is detected. If the detection result of the resuscitation active staining agent is greater than 95% of the total, test tube 31 is removed. If the detection result of the resuscitation active staining agent is less than 95% of the total, the two sets of output shafts of the dual-axis cylinder 21 are activated again, causing the two sets of output shafts of the dual-axis cylinder 21 to extend, causing the lifting support 22 to descend directionally on guide shaft 23 via guide sleeve 24. Test tube 31 is then inserted into warm water to resuscitate the mesenchymal stem cells until the detection result of the resuscitation active staining agent is greater than 95% of the total, at which point test tube 31 is removed.

[0031] Example 2

[0032] like Figures 1 to 4 As shown, in addition to embodiment 1, a heating device 06 and a stirring device 07 are also included;

[0033] The heating device 06 includes a first pipe 61, a power pump 62, a second pipe 63, a heater 64, and a third pipe 65. The first pipe 61 is connected to the side end of the reaction tank 11. The power pump 62 and the heater 64 are both installed on the side end of the reaction tank 11. The input end of the power pump 62 is connected to the output end of the first pipe 61. The output end of the power pump 62 is connected to the input end of the second pipe 63. The output end of the second pipe 63 is connected to the input end of the heater 64. The output end of the heater 64 is connected to the input end of the third pipe 65. The third pipe 65 is connected to the side end of the reaction tank 11.

[0034] The stirring device 07 includes a motor 71 and a motor shaft 72. The motor 71 is installed on the side of the reaction tank 11. The output end of the motor 71 is rotatably connected to the input end of the motor shaft 72. Multiple sets of stirring blades 73 are provided on the motor shaft 72.

[0035] Heating device 06 heats the water, and stirring device 07 stirs the water.

[0036] During the recovery process, the temperature of the water inside the reaction tank 11 is monitored by the temperature monitoring sensor 51. When the temperature does not reach the appropriate temperature, the water inside the reaction tank 11 is introduced into the reaction tank 11 through the input end of the first pipe 61 by starting the power pump 62 and the heater 64. The water flows into the heater 64 through the second pipe 63 and is heated by the heater 64. The heated water flows back into the reaction tank 11 through the third pipe 65. At the same time, the motor 71 is started, which drives the motor shaft 72 and the stirring blade 73 to rotate, stirring the water inside the reaction tank 11 until the water temperature reaches the appropriate recovery temperature. Then the power pump 62 and the heater 64 are stopped.

[0037] This invention discloses a mesenchymal stem cell resuscitation device. During operation, warm water is first poured into the reaction tank 11. Then, mesenchymal stem cells are placed into a test tube 31, along with a resuscitation active staining agent. The test tube 31 is then placed in the through-hole of a lifting support 22. The activation of a dual-axis cylinder 21 extends its two output shafts, causing the lifting support 22 to descend via a guide sleeve 24 on a guide shaft 23, immersing the test tube 31 in the warm water and resuscitating the mesenchymal stem cells. During the resuscitation process, a temperature monitoring sensor 51 monitors the water temperature inside the reaction tank 11. When the temperature is insufficient, a power pump 62 and a heater 64 are activated. Water from the reaction tank 11 enters through the input end of the first pipe 61, flows through the second pipe 63 into the heater 64, and is heated. The heated water then flows back into the reaction tank 11 through the third pipe 65. Simultaneously, a motor 71 is activated. The motor shaft 72 and stirring blade 73 are rotated to agitate the water inside the reaction tank 11, bringing the water temperature to a suitable level for resuscitation. The power pump 62 and heater 64 are then stopped. After the designated resuscitation time, the dual-axis cylinder 21 is activated, causing its two output shafts to retract. This allows the lifting support 22 to rise directionally via the guide sleeve 24 on the guide shaft 23. Then, the resuscitation rate detection instrument 41 is activated to test the viability of the mesenchymal stem cells in the test tube 31. If the proportion of resuscitation active staining is greater than 95%, the test tube 31 is removed. If the proportion is less than 95%, the dual-axis cylinder 21 is activated again, causing its two output shafts to extend. This allows the lifting support 22 to descend directionally via the guide sleeve 24 on the guide shaft 23, inserting the test tube 31 into warm water to resuscitate the mesenchymal stem cells. This process continues until the proportion of resuscitation active staining is greater than 95%, at which point the test tube 31 is removed.

[0038] The reaction tank 11 is transparent, allowing operators to easily observe its interior.

[0039] The dual-axis cylinder 21, recovery rate detection instrument 41, temperature monitoring sensor 51, power pump 62, heater 64 and motor 71 of this utility model are commercially available. Technical personnel in this industry only need to install and operate them according to the accompanying instruction manual, without requiring any creative work from those skilled in the art.

[0040] The main function achieved by this utility model is to maintain the water at a suitable temperature.

[0041] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A device for resuscitating mesenchymal stem cells, comprising a storage device (01); characterized in that, It also includes a lifting device (02), a holding device (03), a detection device (04), a monitoring device (05), a heating device (06), and a stirring device (07). The lifting device (02), the detection device (04), the monitoring device (05), the heating device (06), and the stirring device (07) are all installed on the storage device (01), and the holding device (03) is installed on the lifting device (02). The lifting device (02) lifts and lowers the water, the holding device (03) makes it easy to hold the water, the detection device (04) detects the resuscitation vitality, the monitoring device (05) monitors the temperature, the heating device (06) heats the water, and the stirring device (07) stirs the water.

2. The mesenchymal stem cell resuscitation device as described in claim 1, characterized in that, The storage device (01) includes a reaction tank (11) and a drain pipe (12). The reaction tank (11) is installed in the working area, and the drain pipe (12) is connected to the side end of the reaction tank (11). A valve (13) is provided on the drain pipe (12).

3. The mesenchymal stem cell resuscitation device as described in claim 2, characterized in that, The lifting device (02) includes a dual-axis cylinder (21), a lifting bracket (22), a guide shaft (23), and a guide sleeve (24). The dual-axis cylinder (21) is installed at the top of the reaction tank (11). Two sets of output shafts are provided on the dual-axis cylinder (21). The lifting bracket (22) is installed at the bottom of the two sets of output shafts of the dual-axis cylinder (21). Multiple sets of through holes are provided on the lifting bracket (22). The guide shaft (23) is installed on the reaction tank (11). The guide sleeve (24) is installed on the lifting bracket (22) and is fitted onto the guide shaft (23).

4. The mesenchymal stem cell resuscitation device as described in claim 3, characterized in that, The holding device (03) includes multiple sets of test tubes (31), all of which pass through the through holes of the lifting bracket (22).

5. The mesenchymal stem cell resuscitation device as described in claim 2, characterized in that, The detection device (04) includes a resuscitation rate detection instrument (41), which is installed on the reaction tank (11).

6. The mesenchymal stem cell resuscitation device as described in claim 2, characterized in that, The monitoring device (05) includes a temperature monitoring sensor (51), which is installed on the side of the reaction tank (11).

7. The mesenchymal stem cell resuscitation device as described in claim 2, characterized in that, The heating device (06) includes a first pipe (61), a power pump (62), a second pipe (63), a heater (64), and a third pipe (65). The first pipe (61) is connected to the side of the reaction tank (11). The power pump (62) and the heater (64) are both installed on the side of the reaction tank (11). The input end of the power pump (62) is connected to the output end of the first pipe (61). The output end of the power pump (62) is connected to the input end of the second pipe (63). The output end of the second pipe (63) is connected to the input end of the heater (64). The output end of the heater (64) is connected to the input end of the third pipe (65). The third pipe (65) is connected to the side of the reaction tank (11).

8. The mesenchymal stem cell resuscitation device as described in claim 2, characterized in that, The stirring device (07) includes a motor (71) and a motor shaft (72). The motor (71) is installed on the side of the reaction tank (11). The output end of the motor (71) is rotatably connected to the input end of the motor shaft (72). Multiple sets of stirring blades (73) are provided on the motor shaft (72).

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