Integrated multi-target animal-derived component nucleic acid detection chip

Abstract

The utility model discloses integrated multi -target point animal source component nucleic acid detection chip relates to nucleic acid detection chip technical field, including chip base and sample inlet, the chip base outside is equipped with sample inlet. The utility model discloses integrated multi -target point animal source component nucleic acid detection chip, through being provided with multiple groups above -mentioned detection components on the chip base, thereby handles multiple samples simultaneously, and high -efficiently responds to the demand of large -scale detection, improves the detection efficiency, and the chip base top surface is provided with the sealing cover, is used for closing micro -flow path and chamber, prevents sample evaporation, pollution and the outside contamination and enters, through the multiple pretreatment to sample before entering the mixed cavity, and then improves the detection sensitivity, so that the subsequent detection can detect trace animal source component in the sample, and then improves the detection precision, guarantees the accuracy of detection result.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection chip technology, specifically an integrated multi-target animal-derived component nucleic acid detection chip. Background Technology

[0002] Nucleic acid detection chips, also known as gene chips or DNA microarrays, are integrated biological detection devices. Integrated multi-target animal-derived component nucleic acid detection chips are an automated, high-throughput detection technology that integrates multiple steps such as nucleic acid hybridization, enzyme labeling, washing, color development, and result scanning. They are primarily used for the rapid and accurate identification of animal-derived components and are crucial in areas such as food safety, species identification, and import / export inspection and quarantine.

[0003] In the current use of nucleic acid detection chips, after the sample is introduced into the chip, the sample is usually not amplified or lysed, resulting in poor sensitivity of subsequent detection, which affects the detection quality and makes it difficult to efficiently meet the demand for large-scale testing. Utility Model Content

[0004] The purpose of this invention is to provide an integrated multi-target animal-derived component nucleic acid detection chip to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: an integrated multi-target animal-derived component nucleic acid detection chip, comprising a chip base and an inlet. The inlet is provided on the outer side of the chip base. The output end of the inlet is connected to an inlet channel, and a magnetic bead lysis and binding chamber is connected to one side of the outer wall of the inlet channel. The output end of the magnetic bead lysis and binding chamber is connected to a first microfluidic channel, and a pretreatment chamber is connected to one side of the outer wall of the first microfluidic channel. The output end of the pretreatment chamber is connected to a purification chamber, and the output end of the purification chamber is connected to a mixing chamber through a delivery channel. The output end of the mixing chamber is connected to a third microfluidic channel, and a reaction chamber is connected to one side of the outer wall of the third microfluidic channel. The reaction chamber is connected to a waste liquid chamber through an outlet pipe. The sample inlet and the reaction chamber are both distributed at equal angles, thus enabling the simultaneous processing of multiple animal samples, meeting the needs of large-scale testing and improving testing efficiency. The magnetic bead lysis binding chamber breaks down cells and releases DNA through the interaction of the lysis solution and magnetic beads, thereby improving detection accuracy.

[0006] By pre-processing the sample multiple times before it enters the mixing chamber, the detection sensitivity is improved, enabling subsequent detection to detect trace amounts of animal-derived components in the sample, thereby improving detection accuracy and ensuring the accuracy of the detection results.

[0007] Preferably, the inlet is equipped with a micro-filter membrane to remove large particulate impurities, and the first, second and third microfluidic channels all use capillary action, centrifugal force or external micro-pumps to precisely control the flow of samples and reagents.

[0008] Large particulate impurities are removed by a micro-filter membrane inside the injection port. The sample then enters the magnetic bead lysis and binding chamber through the injection channel. Cells are broken up and DNA is released by the action of the lysis buffer and magnetic beads inside the magnetic bead lysis and binding chamber. The DNA is then purified by magnetic control.

[0009] Preferably, the pretreatment chamber can perform preliminary nucleic acid amplification to increase the number of target sequences, and the purification chamber purifies the crude extract to remove PCR inhibitors.

[0010] The sample undergoes preliminary nucleic acid amplification via the pretreatment chamber to increase the number of target sequences and improve the sensitivity of subsequent detection; the sample is then purified via the purification chamber to remove PCR inhibitors and obtain pure DNA.

[0011] Preferably, a detection window is provided above the reaction chamber to facilitate external optical detection equipment to excite fluorescence signals and collect data, and specific nucleic acid probes for different animal-derived components are pre-embedded inside the reaction chamber.

[0012] The reaction chamber provides a site for specific nucleic acid hybridization or amplification reactions. When sample DNA flows through the top surface of the reaction chamber, a sealing cap is connected to close the microchannel and chamber. The waste liquid chamber collects and stores the reaction solution and washing solution that have completed the reaction.

[0013] The microchannels and chambers are sealed with a cap to prevent sample evaporation, contamination, and the entry of external contaminants.

[0014] As can be seen from the above, the integrated multi-target animal-derived component nucleic acid detection chip provided by this utility model has the following beneficial effects.

[0015] 1. By setting multiple sets of the above-mentioned detection components on the chip base, multiple samples can be processed simultaneously, which can efficiently meet the needs of large-scale detection and improve detection efficiency. The top surface of the chip base is equipped with a sealing cover to seal the microchannels and chambers, preventing sample evaporation, contamination and the entry of external pollutants.

[0016] 2. By pre-treating the sample multiple times before it enters the mixing chamber, the detection sensitivity is improved, enabling subsequent detection to detect trace amounts of animal-derived components in the sample, thereby improving detection accuracy and ensuring the accuracy of the detection results. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall three-dimensional structure of the present invention; Figure 2 This is a schematic diagram of the main structure of this utility model; Figure 3 This is a schematic diagram of the sample detection process structure of this utility model.

[0018] In the diagram: 1. Chip base; 2. Inlet port; 3. Inlet channel; 4. Magnetic bead lysis and binding chamber; 5. Microfluidic channel 1; 6. Pretreatment chamber; 7. Microfluidic channel 2; 8. Purification chamber; 9. Mixing chamber; 10. Microfluidic channel 3; 11. Reaction chamber; 12. Waste liquid chamber. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Please see Figures 1-3 This utility model provides a technical solution: an integrated multi-target animal-derived component nucleic acid detection chip, including a chip base 1 and an inlet 2. The inlet 2 is provided on the outer side of the chip base 1. The output end of the inlet 2 is connected to an inlet channel 3. A magnetic bead lysis and binding chamber 4 is connected to one side of the outer wall of the inlet channel 3. The output end of the magnetic bead lysis and binding chamber 4 is connected to a first microfluidic channel 5. A pretreatment chamber 6 is connected to one side of the outer wall of the first microfluidic channel 5. The output end of the pretreatment chamber 6 is connected to a purification chamber 8. The output end of the purification chamber 8 is connected to a mixing chamber 9 through a delivery channel. The output end of the mixing chamber 9 is connected to a third microfluidic channel 10. A reaction chamber 11 is connected to one side of the outer wall of the third microfluidic channel 10. The reaction chamber 11 is connected to a waste liquid chamber 12 through an outlet pipe. The sample inlet 2 and the reaction chamber 11 are both distributed at equal angles, thus processing multiple animal samples simultaneously, meeting the needs of large-scale testing and improving testing efficiency. The magnetic bead lysis chamber 4 breaks down cells and releases DNA through the interaction of the lysis buffer and magnetic beads, thereby improving detection accuracy. The sample inlet 2 is equipped with a microfiltration membrane to remove large particulate impurities. Microfluidic channels 5, 7, and 10 all precisely control the flow of samples and reagents through capillary action, centrifugal force, or an external micropump. The pretreatment chamber 6 performs preliminary nucleic acid amplification to increase the number of target sequences. The purification chamber 8 purifies the crude extract and removes PCR inhibitors. A detection window is located above the reaction chamber 11 to facilitate the excitation of fluorescence signals and data acquisition by external optical detection equipment. Specific nucleic acid probes for different animal-derived components are pre-embedded inside the reaction chamber 11. A sealing cap is connected to the top surface of the chip base 1 to seal the microchannels and chambers. The waste liquid chamber 12 collects and stores the completed reaction solution and washing solution.

[0021] In practice, when performing nucleic acid testing on animal-derived components, the sample is first introduced into the chip base 1 through the injection port 2, and then the extracted DNA sample to be tested and subsequent reaction reagents are introduced. At this time, large particulate impurities are removed through the micro-filter membrane inside the injection port 2. Then, the sample reaches the magnetic bead lysis binding chamber 4 through the injection channel 3. The cells are broken and DNA is released by the action of the lysis buffer and magnetic beads inside the magnetic bead lysis binding chamber 4. Finally, the DNA is purified by magnetic control. The sample in chamber 4 is then lysed by magnetic beads and reaches pretreatment chamber 6 through microfluidic channel 5. Pretreatment chamber 6 performs initial nucleic acid amplification to increase the number of target sequences and improve the sensitivity of subsequent detection. Next, the sample in pretreatment chamber 6 reaches purification chamber 8 through microfluidic channel 7. Purification chamber 8 purifies the sample, removing PCR inhibitors and obtaining pure DNA. The processed sample is then introduced into mixing chamber 9. This multiple pretreatment process before the sample enters mixing chamber 9 further improves detection quality. After the sample enters the mixing chamber 9, it is thoroughly mixed with the PCR premix or hybridization reagent to ensure the uniformity of the reaction. After the sample is mixed, it enters the reaction chamber 11 through the third microfluidic channel 10. The reaction chamber 11 provides a site for specific nucleic acid hybridization or amplification reactions. When the sample DNA flows through the reaction chamber 11, if the DNA sequence of the target animal-derived component is present, specific binding or amplification will occur, and fluorescence will be generated. External optical detection devices, such as fluorescence microscopes and CCD cameras, will excite fluorescence signals and collect data. The reaction liquid and cleaning liquid that have completed the reaction are then collected and stored through the waste liquid chamber 12 to prevent waste liquid residue in the reaction area from interfering with the detection results. The top surface of the chip base 1 is provided with a sealing cover to seal the microchannel and chamber, preventing sample evaporation, contamination and the entry of external pollutants.

[0022] By setting multiple sets of the above-mentioned detection components on the chip base 1, multiple samples can be processed simultaneously, efficiently meeting the needs of large-scale detection and improving detection efficiency. Furthermore, by improving detection sensitivity, subsequent detection can detect trace amounts of animal-derived components in the sample, thereby improving detection accuracy and ensuring the accuracy of the detection results.

[0023] The above description is merely a preferred embodiment of this utility model, but the protection scope of this utility model is not limited thereto. The substitutions may be replacements of some structures, devices, or method steps, or they may be complete technical solutions. Equivalent substitutions or modifications made based on the technical solution and inventive concept of this utility model should all be covered within the protection scope of this utility model.

Claims

1. An integrated multi-target animal-derived component nucleic acid detection chip, comprising a chip base (1) and a sample inlet (2), wherein the sample inlet (2) is provided on the outer side of the chip base (1), characterized in that: The inlet (2) is connected to an inlet channel (3) at its output end, and a magnetic bead lysis and binding chamber (4) is connected to one side of the outer wall of the inlet channel (3). The output end of the magnetic bead lysis and binding chamber (4) is connected to a first microfluidic channel (5), and a pretreatment chamber (6) is connected to one side of the outer wall of the first microfluidic channel (5). The output end of the pretreatment chamber (6) is connected to a purification chamber (8), and the output end of the purification chamber (8) is connected to a mixing chamber (9) through a delivery channel. The output end of the mixing chamber (9) is connected to a third microfluidic channel (10), and a reaction chamber (11) is connected to one side of the outer wall of the third microfluidic channel (10). The reaction chamber (11) is connected to a waste liquid chamber (12) through an outlet pipe. The inlet (2) and the reaction chamber (11) are both distributed at equal angles, thus processing multiple animal samples simultaneously, meeting the needs of large-scale testing, and improving testing efficiency; The magnetic bead lysis binding chamber (4) breaks cells and releases DNA through the action of lysis fluid and magnetic beads, thereby improving detection accuracy.

2. The integrated multi-target animal-derived component nucleic acid detection chip according to claim 1, characterized in that: The inlet (2) is equipped with a micro-filter membrane to remove large particulate impurities. The first microfluidic channel (5), the second microfluidic channel (7) and the third microfluidic channel (10) all use capillary action, centrifugal force or external micro-pumps to precisely control the flow of samples and reagents.

3. The integrated multi-target animal-derived component nucleic acid detection chip according to claim 1, characterized in that: The pretreatment chamber (6) can perform preliminary nucleic acid amplification to increase the number of target sequences, and the purification chamber (8) purifies the crude extract to remove PCR inhibitors.

4. The integrated multi-target animal-derived component nucleic acid detection chip according to claim 1, characterized in that: A detection window is provided above the reaction chamber (11) to facilitate the excitation of fluorescence signals and data collection by external optical detection equipment. Specific nucleic acid probes for different animal-derived components are pre-embedded inside the reaction chamber (11).

5. The integrated multi-target animal-derived component nucleic acid detection chip according to claim 1, characterized in that: The chip base (1) has a sealing cap on its top surface to seal the microchannel and chamber. The waste liquid chamber (12) collects and stores the reaction liquid and cleaning liquid that have completed the reaction.

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