A sample detection device

Abstract

A sample detection device includes a sampling module, a detection module and a processing module, the sampling module is used for injecting a first part of a sample to a first processing position for staining treatment, and then injecting the stained first part of the sample to a first detection channel; the sampling module is also used for directly or indirectly injecting a second part of the sample to a second detection channel of a sample test carrier. The sample detection device realizes staining and non-staining treatment of the same sample at the same time, so that the detection results of the stained and non-stained samples can be obtained at the same time, thereby covering more detection items; the sample detection device injects the stained and non-stained samples into two independent detection channels respectively for detection, so that the stained and non-stained samples are independent of each other and do not interfere with each other, and the accuracy of the detection of the stained and non-stained samples can be ensured.

Description

Technical Field

[0001] This utility model relates to the field of biochemical detection technology, specifically to a sample detection device. Background Technology

[0002] In current clinical diagnostics, in vitro diagnostic products mainly include instrumental detection for precise counting and microscopic detection, which is primarily done manually. During microscopic detection, different target analytes in the sample need to be detected. To achieve accurate differentiation, multiple slides are often prepared. One method is to directly dilute the sample with physiological saline and spread it onto the slide, mainly for observing the sample's actual state and identifying analytes that are visible without staining, such as parasites. Another method is to stain the sample with special reagents, mainly for observing microscopic analytes, such as cells and bacteria. However, this approach significantly increases the complexity of clinical procedures. Preparing a single sample slide often takes 15-20 minutes, and there are biosafety risks involved. After preparation, a professional reader is required to interpret the slides, identifying the various complex components in the sample. This requires extensive reading experience and is very time-consuming; a complete and accurate interpretation of a single sample slide often takes 5-10 minutes. Prolonged microscopic observation can easily damage the eyes.

[0003] Currently, some manufacturers have explored the automation of microscopic examination. Among the existing automation solutions, some manufacturers have achieved automatic slide reading, but manual slide preparation is still required. The complex slide preparation process has not been solved. Moreover, manual staining can lead to inconsistent smear positions and staining effects, making it difficult to identify the optimal staining area, increasing the complexity of clinical operations, and making it difficult to guarantee detection accuracy. Utility Model Content

[0004] This invention proposes a sample detection device to solve the problems of complex manual operation and difficulty in guaranteeing detection accuracy.

[0005] In some embodiments, a sample detection device is provided, comprising:

[0006] The sampling module is used to inject a first portion of the sample into a first processing position of the sample test carrier for staining, and then inject the stained first portion of the sample into a first detection channel of the sample test carrier; the sampling module is also used to inject a second portion of the sample directly or indirectly into a second detection channel of the sample test carrier, wherein the first portion of the sample and the second portion of the sample belong to the same sample.

[0007] The detection module is used to take pictures of the first portion of the sample after staining in the first detection channel to obtain a first image; it is also used to take pictures of the second portion of the sample that is not stained in the second detection channel to obtain a second image.

[0008] The system also includes a processing module connected to the detection module. The processing module is used to acquire the first image and the second image, process the first image to obtain the detection result of the stained test object, and process the information of the second image to obtain the detection result of the unstained test object.

[0009] The first detection channel and the second detection channel are independent of each other, and the first part of the stained sample can show clear staining characteristics of the analyte.

[0010] In some embodiments, the processing module processes the first image to obtain at least detection results related to cells and / or bacteria, and the processing module processes the second image to obtain at least detection results related to parasites and / or digestive products.

[0011] In some embodiments, the sampling module is used to draw up a sample and inject the first portion of the drawn up sample into the first processing position of the sample test carrier for staining, and inject the second portion of the drawn up sample into the second detection channel of the sample test carrier.

[0012] In some embodiments, the detection module is a microscope detection module.

[0013] In some embodiments, the sample detection device is used to detect a sample in a sample test carrier that includes at least the first detection channel and the second detection channel.

[0014] In some embodiments, a sample testing carrier is also included, wherein one of the sample testing carriers includes at least the first detection channel and the second detection channel.

[0015] In some embodiments, the first detection channel and the second detection channel are cavities for containing liquids or suspensions.

[0016] In some embodiments, no reagents are pre-placed in the first and second detection channels.

[0017] In some embodiments, both the first detection channel and the second detection channel include an injection port, a detection chamber, and an exhaust port that are connected in sequence, and at least the upper wall of the detection chamber is a transparent structure.

[0018] In some embodiments, the detection cavity is a flat cavity, which is used to present the first portion of the sample or the second portion of the sample as a thin liquid layer.

[0019] In some embodiments, a microfluidic channel is connected between the injection port and the detection chamber, the microfluidic channel being used to siphon the first portion of the sample or the second portion of the sample into the detection chamber.

[0020] In some embodiments, the sample test carrier is a disposable consumable, and the sample test carrier can be detachably installed on the sample detection device.

[0021] In one embodiment, the sample includes one of feces, urine, skin, ocular secretions, ear canal secretions, and pleural or peritoneal fluid.

[0022] The sample detection device according to the above embodiment, having a sampling module, a detection module, and a processing module, allows the sampling module to dispense the same sample into different detection channels for detection, placing analytes requiring staining and those not requiring staining in different channels. The detection module can take pictures of samples with stained and unstained analytes respectively, obtaining a first image and a second image. The processing module can process the first and second images to obtain the detection results for the stained and unstained analytes. The sample detection device enables simultaneous staining and unstaining of the same sample, thereby simultaneously obtaining detection results for both stained and unstained analytes, thus covering a wider range of detection items.

[0023] Secondly, the sample detection device can automate the detection of both stained and unstained samples, resulting in high detection efficiency. It can avoid errors caused by manual detection and prevent damage to personnel during the manual detection process.

[0024] Furthermore, this sample detection device injects the stained and unstained analytes into two independent detection channels for detection, ensuring that the stained and unstained analytes are independent of each other and do not interfere with each other, thus guaranteeing the accuracy of detecting the stained and unstained analytes separately. Attached Figure Description

[0025] Figure 1 These are schematic diagrams of the sample detection device in some embodiments;

[0026] Figure 2 This is a schematic diagram of the structure of the sample test carrier in some embodiments;

[0027] Figure 3 This is a schematic diagram of the structure of the sample test carrier in some embodiments;

[0028] Figure 4 This is a schematic diagram of the structure of the sample test carrier in some embodiments;

[0029] Figure 5 This is a schematic diagram of the structure of the sample test carrier in some embodiments;

[0030] Figure 6 This is a schematic diagram of the structure of the sample test carrier in some embodiments;

[0031] Figure 7 This is a schematic diagram of the structure of the sample test carrier in some embodiments;

[0032] Figure 8 This is a schematic diagram of the structure of the sample test carrier in some embodiments;

[0033] Figure 9 This is a partial structural schematic diagram of the sample test carrier in some embodiments;

[0034] Figure 10 Here are flowcharts of sample detection methods in some embodiments;

[0035] Figure 11 Here are flowcharts of sample detection methods in some embodiments;

[0036] Figure 12 Here are flowcharts of sample detection methods in some embodiments;

[0037] Figure 13 Here are flowcharts of sample detection methods in some embodiments;

[0038] Figure 14 Here are flowcharts of sample detection methods in some embodiments;

[0039] Figure 15 Here are flowcharts of sample detection methods in some embodiments;

[0040] Figure 16 Here are flowcharts of sample detection methods in some embodiments;

[0041] The accompanying diagrams are labeled as follows:

[0042] 1-Sampling module, 11-Sampling device, 12-Motion device;

[0043] 2-Detection module;

[0044] 3-Processing module;

[0045] 4-Sample test carrier, 410-Sample storage position, 421-First reagent storage position, 422-Second reagent storage position, 431-First processing position, 432-Second processing position, 441-First detection channel, 442-Second detection channel; 4411-Injection port, 4412-Detection chamber, 4413-Exhaust port, 4414-Microfluidic channel;

[0046] a- Sample placement area, b- Reagent storage area, c- Reaction area, d- Detection area. Detailed Implementation

[0047] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments. Similar elements in different embodiments are referred to by related similar element reference numerals. In the following embodiments, many details are described to facilitate a better understanding of the present application. However, those skilled in the art will readily recognize that some features may be omitted in different situations, or may be replaced by other elements, materials, or methods. In some cases, certain operations related to the present application are not shown or described in the specification. This is to avoid obscuring the core parts of the present application with excessive description. For those skilled in the art, detailed description of these related operations is not necessary; they can fully understand the related operations based on the description in the specification and general technical knowledge in the art.

[0048] Furthermore, the features, operations, or characteristics described in the specification can be combined in any suitable manner to form various embodiments. At the same time, the steps or actions in the method description can be rearranged or adjusted in a manner obvious to those skilled in the art. Therefore, the various orders in the specification and drawings are only for the clear description of a particular embodiment and do not imply a necessary order, unless otherwise stated that a particular order must be followed.

[0049] The serial numbers assigned to components in this document, such as "first" and "second," are used only to distinguish the objects being described and have no sequential or technical meaning. Unless otherwise specified, the terms "connection" and "linkage" used in this application include both direct and indirect connections (linkages).

[0050] In some embodiments, a sample detection device is provided, which can detect at least two analytes in a sample. This device can automatically perform staining and other pretreatment on the sample, and can perform dual independent channel detection on stained and unstained samples, avoiding mixing and interference between stained and unstained samples. It enables the simultaneous detection of stained and unstained analytes on the same sample, obtaining detection results for both stained and unstained analytes, thereby covering more detection items. Furthermore, it achieves automated detection while ensuring accuracy.

[0051] This sample testing device can test various samples, such as feces, urine, skin, eye secretions, ear canal secretions, and pleural or peritoneal fluid. The samples that can be processed by the device can include, but are not limited to, one of these: feces, urine, skin, eye secretions, ear canal secretions, or pleural or peritoneal fluid. This device primarily detects analytes in the above-mentioned samples by staining or leaving them unstained. These analytes can include, but are not limited to, cells and bacteria, parasites, and digestive products.

[0052] The analytes in the samples are mainly divided into two categories. One category consists of analytes requiring staining. These analytes and their characteristics are relatively blurry in the environmental liquid of the sample, making it difficult to distinguish them directly through visual imaging, thus hindering identification. Examples include analytes such as leukocytes and bacteria, where the nucleus characteristics of leukocytes are difficult to identify. These analytes require staining before detection, as staining allows for clearer staining characteristics, enabling clear visual identification. The other category consists of analytes that do not require staining. These analytes and their characteristics are relatively clear in the environment and can be directly distinguished visually. Examples include analytes such as parasites and digestive products. These analytes do not require staining before detection and can be directly visually inspected. Of course, both analytes requiring staining and those not requiring staining can undergo other pretreatments before detection, such as dilution, to improve detection accuracy.

[0053] Please refer to Figure 1 In some embodiments, the sample detection device mainly includes a sampling module 1, a detection module 2, and a processing module 3.

[0054] The sample testing device can be a closed testing device, and may also include housing components such as a chassis. The chassis has a cavity within it, where the sampling module 1, detection module 2, and processing module 3 are installed. The chassis provides isolation and protection for the sampling module 1, detection module 2, and processing module 3, preventing external interference during the testing process. Simultaneously, the chassis can provide a mounting and support structure to secure the sampling module 1, detection module 2, and processing module 3 as a single unit. The chassis may also include an observation area for personnel to monitor the testing process.

[0055] In other embodiments, the sample detection device may also be an open or semi-open detection device, having an open platform at which the sampling module 1, detection module 2, and processing module 3 are mounted on the upper or lower end. An open sample detection device allows testing personnel to directly observe the testing process and monitor it in real time.

[0056] In this embodiment, the sampling module 1 may include a sampling device 11 and a motion device 12. The sampling device 11 may be a device that can dispense samples using a needle or disposable tip, etc. The sampling device 11 is connected to a power source such as a vacuum pump. The sampling device 11 can dispense samples by suction and discharge, and the suction and discharge of the sampling device 11 can also be used for sample mixing. The sampling device 11 is mounted on the motion device 12, which may be a two-dimensional or three-dimensional motion device. For example, the motion device 12 can move in three-dimensional space along the XYZ axes. The motion device 12 is used to drive the sampling device 11 to perform translation, lifting, and rotation movements to achieve sample transfer and dispensing.

[0057] The driving component of the motion device 12 can be a motor or a cylinder, etc. The motion device 12 may also include a position sensor to improve the accuracy of the spatial movement of the sampling device 11, thereby improving the accuracy of sample transfer.

[0058] The sampling device 11 is used to dispense samples into different detection channels to achieve sample transfer. For example, the sampling device 11 is used to dispense a first portion of the sample into a first processing position for staining, and the sampling device 11 is also used to dispense the stained first portion of the sample into a first detection channel, thus completing the dispensing of the sample to be stained. The sampling device 11 can also indirectly dispense a second portion of the sample that does not require pretreatment into a second detection channel. For example, the sampling device 11 can first dispense the second portion of the sample into a second processing position for pretreatment, and then dispense the pretreated second portion into the second detection channel, or the sampling device 11 can directly dispense the second portion of the sample that does not require pretreatment into the second detection channel, thus completing the dispensing of the wirelessly stained sample to be stained. The first portion of the sample and the second portion of the sample belong to the same sample.

[0059] The sampling module 1 may include a sampling device 11. The sampling device 11 is used to dispense a first portion of the sample into a first processing position for staining, and then transfer the stained first portion of the sample to a first detection channel for detection. The same sampling device 11 is also used to dispense a second portion of the sample into a second processing position for unstained pre-processing, and then transfer the pre-processed second sample to a second detection channel for detection. Alternatively, the same sampling device 11 can directly dispense the second portion of the sample into the second detection channel for detection. The same sampling device 11 can draw a sufficient amount of sample at once and dispense the drawn sample into the first and second processing positions, or into the first processing position and the second detection channel. Using a single draw and multiple dispensing method can improve detection efficiency. Of course, a single sampling device 11 can also draw samples multiple times, for example, drawing the first portion of the sample in the first draw and the second portion in the second draw. Using multiple draws and multiple dispensing methods can also dispense the sample into the corresponding positions for stained and unstained detection.

[0060] In other embodiments, the sampling module 1 may further include multiple sampling devices 11, which are installed independently of each other. The multiple sampling devices 11 can dispense samples synchronously or asynchronously to improve detection efficiency. For example, two sampling devices 11 are provided. One sampling device 11 is used to dispense the first part of the sample, dispense the first part of the sample to the first processing position for staining, and then transfer the stained first part of the sample to the first detection channel for detection. The other sampling device 11 is used to dispense the second part of the sample, dispense the second part of the sample to the second processing position for unstained pretreatment, and then transfer the pretreated second part of the sample to the second detection channel. Alternatively, the other sampling device 11 can directly dispense the second part of the sample into the second detection channel.

[0061] In this embodiment, detection module 2 is a visual detection module, such as a microscope detection module. Detection module 2 can magnify and image the sample to obtain information such as the number and outline of tiny test objects in the sample. Detection module 2 can automatically locate, focus, and scan the sample. Detection module 2 is used to photograph samples in multiple detection channels to generate corresponding images. For example, detection module 2 photographs two detection channels: the first detection channel is used to inject a sample containing stained test objects, and photographing the sample in the first detection channel forms a first image; the second detection channel is used to inject a sample containing unstained test objects, and photographing the sample in the second detection channel forms a second image.

[0062] The sample detection device may also include a mobile detection module 2, which takes pictures of the test objects in the two detection channels by moving.

[0063] In other embodiments, the sample detection device may include multiple detection modules 2, such as two fixedly installed detection modules 2, which are configured one-to-one with two detection channels to take pictures of different test objects in the detection channels.

[0064] In this embodiment, the processing module 3 is signal-connected to the detection module 2. The processing module 3 is used to acquire the image formed by the detection module 2, and to process and analyze the image to obtain information about the object to be tested.

[0065] For example, the processing module 3 is used to acquire a first image formed by the detection module 2 taking pictures of a sample with stained test material and a second image formed by taking pictures of a sample with unstained test material. The processing module 3 analyzes the stained test material in the first image to obtain at least the detection results related to cells and / or bacteria (stained test material). The processing module 3 processes and analyzes the unstained test material in the second image to obtain at least the detection results related to parasites and / or digestive products (unstained test material).

[0066] The sample detection device of this embodiment is used in conjunction with the sample test carrier 4. Each sample test carrier 4 includes at least two independent detection channels, for example, at least a first detection channel 441 and a second detection channel 442, to detect the analyte in the sample. The sample test carrier 4 can be used as a disposable consumable. The sample detection device may or may not include the sample test carrier 4. A carrying platform is provided within the accommodating cavity of the sample detection device, and the sample test carrier 4 can be placed on or fixed to the carrying platform. The carrying platform may be provided with a positioning structure or a fixing structure to fix the sample test carrier 4 at a preset position, improving the sample transfer accuracy of the sampling module 1 and the imaging accuracy of the detection module 2.

[0067] The sample detection device in this embodiment may include a control module or processing module 3 that also functions as a control module. The control module or processing module 3 is electrically connected to the sampling module 1 and the detection module 2. The control module or processing module 3 is used to control the sampling module 1 to sample and to control the detection module 2 to capture images. The control module or processing module 3 may have one or more different detection modes, wherein multiple different detection modes are used to correspond to multiple different sample test carriers 4, so as to improve the compatibility of the sample detection device and meet the detection requirements of diverse sample test carriers 4.

[0068] Please refer to Figure 1 andFigure 2 The sample testing carrier 4 can be a sheet-like or plate-like structure. A sample testing carrier 4 mainly includes a sample storage position 410, a first processing position 431, a first detection channel 441, and a second detection channel 442. The sample storage position 410, the first processing position 431, the first detection channel 441, and the second detection channel 442 are all independent structures. The sample storage position 410 and the first processing position 431 are accommodating cavities. The sample storage position 410 is used to hold the sample, the first processing position 431 provides a space for sample staining, the first detection channel 441 presents the stained sample within a very thin planar cavity, and the second detection channel 442 presents the unstained sample within a very thin planar cavity. The stained first part of the sample and the unstained second part of the sample are ultimately presented within the very thin planar cavity, allowing the analyte to be distributed within a plane for imaging, making the analyte more clearly detected and identified.

[0069] In this embodiment, the sampling module 1, detection module 2, and processing module 3 are included. The sampling module 1 can dispense the same sample from the sample test carrier 4 into different detection channels for detection, placing analytes requiring staining and those not requiring staining in different detection channels. The detection module 2 can take pictures of the sample with stained analytes and the sample with unstained analytes to obtain a first image and a second image, respectively. The processing module 3 can process the first and second images to obtain the detection results for stained and unstained analytes. The sample detection device enables simultaneous staining and unstaining of the same sample, thereby simultaneously obtaining detection results related to cells and / or bacteria (stained analytes) and detection results related to parasites and / or digestive products (unstained analytes), thus covering more detection items.

[0070] Secondly, the sample detection device can automate the detection of both stained and unstained samples, resulting in high detection efficiency. It can avoid errors caused by manual detection and prevent damage to personnel during the manual detection process.

[0071] Furthermore, this sample detection device injects the stained and unstained analytes into two independent detection channels on the sample test carrier 4 for detection, ensuring that the stained and unstained analytes are independent of each other and do not interfere with each other, thus guaranteeing the accuracy of detecting the stained and unstained analytes separately.

[0072] In some embodiments, the detection module 2 and the processing module 3 may adopt the same structure as in the prior art, as well as the same optical imaging method and image processing method, to obtain the detection results of stained and unstained test objects.

[0073] In some embodiments, the sample detection device further includes a cleaning device, which may include a cleaning solution container connected to a pipe of the sampling device 11. The cleaning device may be a cleaning solution for cleaning the sampling device 11. The sampling device may perform cleaning by sampling and aspirating the cleaning solution, thereby avoiding cross-contamination during the sample dispensing process of the sampling device 11 and ensuring the accuracy of the detection.

[0074] Please refer to Figure 1 and Figure 2 In some embodiments, the sample test carrier 4 includes a sample storage location 410, a first processing location 431, a first detection channel 441, and a second detection channel 442.

[0075] The sample storage bits 410 may include one or more, and the number of sample storage bits 410 can be set according to requirements.

[0076] The first processing position 431 is pre-filled with staining reagent, while the first detection channel 441 and the second detection channel 442 are not pre-filled with staining reagent or other reagents. The first processing position 431 may also be provided with a film or other sealing cap, which covers the staining reagent inside the first processing position 431. The sealing cap can be torn open during use, or the sampling device 11 can directly puncture the sealing cap.

[0077] The first processing position 431 is pre-loaded with a sample test carrier 4 containing staining reagent. The sample detection device has a corresponding detection mode, which includes the following method steps:

[0078] The sampling device 11 dispenses the first part of the sample stored in the sample storage position 410 into the first processing position 431; the first part of the sample reacts with the staining reagent in the first processing position 431, and the staining reagent stains the analyte in the first part of the sample, so that the analyte after staining can show clear staining characteristics.

[0079] The sampling device 11 dispenses the first portion of the stained sample into the first detection channel 441;

[0080] The sampling device 11 dispenses the second part of the sample stored in the sample storage position 410 into the second detection channel 442, wherein the analyte in the second part of the sample does not require staining.

[0081] The detection module 2 takes a picture of the first part of the stained sample in the first detection channel 441 to obtain a first image; and takes a picture of the second part of the unstained sample in the second detection channel 442 to obtain a second image.

[0082] Processing module 3 acquires a first image and a second image, processes the first image to obtain the detection result of the stained test object, and processes the information of the second image to obtain the detection result of the unstained test object.

[0083] If the sample detection device is equipped with a sampling device 11, the second part of the sample does not need to be pre-treated. The sampling device 11 can first dispense the second part of the sample that does not need to be stained into the second detection channel 442, then dispense the first part of the sample into the first processing position 431, and finally dispense the stained first part of the sample into the first detection channel 441. With this configuration, the sampling device 11 does not need to be cleaned and can complete the dispensing of the first part of the sample and the second part of the sample. The dispensing efficiency is high and cross-contamination can be avoided.

[0084] In other embodiments, the sampling device 11 may first dispense the first part of the sample and then dispense the second part of the sample. After dispensing the stained first part of the sample, it is cleaned and then the second part of the sample is dispensed.

[0085] In this embodiment, if the sample detection device is equipped with two sampling devices 11, one sampling device 11 dispensing the first part of the sample and the other sampling device dispensing the second part of the sample, then the dispensing of the first part of the sample and the second part of the sample can be performed simultaneously or asynchronously.

[0086] In this test, the first and second portions of the sample are from the same sample, and the contents of the first and second portions are identical. During testing, the volume of liquid dispensed into the first and second portions can be the same or different. For example, the sample storage compartment 410 of the sample test carrier 4 may contain 10 ml of urine sample, with the first portion being 4 ml and the second portion being either 4 ml or 6 ml. During the test, the urine sample in the sample storage compartment 410 can be used entirely or only partially.

[0087] The first processing position 431 is pre-filled with staining reagent. The first processing position 431 has two functions: staining reagent storage and staining treatment. It eliminates the step of dispensing the staining reagent into the first processing position 431 first, simplifies the dispensing steps of the sampling device 11, and helps to improve detection efficiency.

[0088] In some embodiments, please refer to Figure 3 In some embodiments, the sample test carrier 4 differs from the sample test carrier 4 described above in that the sample test carrier 4 does not have a sample storage bit 410.

[0089] In this embodiment, the sample detection device has sample racks or other sample storage locations on its inner or outer side. The sampling device 11 of the sampling module 1 can pick up samples from the sample racks or other sample storage locations and then transfer and dispense the samples onto the sample test carrier 4. It can also dispense the first part of the same sample onto the sample test carrier 4 for staining and detection, and dispense the second part of the sample onto the sample test carrier 4 for unstained processing and inspection.

[0090] Please refer to Figure 1 and Figure 4 In some embodiments, the sample test carrier 4 differs from the sample test carrier 4 described above in that a first reagent storage position 421 is added, and the first processing position 431 is not pre-filled with staining reagent.

[0091] The first reagent storage position 421 is pre-filled with staining reagent. The first reagent storage position 421 may also be provided with a film or other sealing cap. The sealing cap covers the staining reagent inside the first reagent storage position 421. When in use, the sealing cap can be torn open, or the sampling device 11 can directly puncture the sealing cap.

[0092] The sample test carrier 4 is equipped with a first reagent storage position 421. The sample detection device has a corresponding detection mode. The method steps included in this detection mode are different from those in the above embodiments in that a staining reagent dispensing step is added.

[0093] Specifically: the sampling device 11 of the sampling module dispenses the first portion of the sample stored in the sample storage position 410 into the first processing position 431 without pre-filled staining reagent; the sampling device 11 of the sampling module is also used to dispense the staining reagent in the first reagent storage position 421 into the first processing position 431 without pre-filled staining reagent. The order of the two steps, dispensing the first portion of the sample and dispensing the staining reagent, is not limited and can be set according to actual needs.

[0094] The sample test carrier 4 is additionally provided with a first reagent storage position 421, which places the staining reagent in a position other than the first processing position 431, so that the first processing position 431 can provide a place for other pretreatments besides staining, and can increase other functions and uses of the sample test carrier 4.

[0095] Please refer to Figure 1 , Figure 5 and Figure 6 In some embodiments, the sample test carrier 4 is provided with a second processing bit 432 on the basis of the sample test carrier 4 of any of the above embodiments.

[0096] The second processing position 432 is pre-filled with pretreatment reagents, which may include, but are not limited to, dilution reagents and separation reagents. The second processing position 432 may also be equipped with a cap such as a film, which covers the staining reagent inside the second processing position 432. The cap can be torn open during use, or the sampling device 11 can directly puncture the cap.

[0097] The second processing position 432 is pre-loaded with a sample test carrier 4 containing pretreatment reagents. The sample detection device has a corresponding detection mode. The method steps included in this detection mode differ from those in the above embodiments in that a second sample pretreatment step is added.

[0098] Specifically: the sampling device 11 of the sampling module 1 first dispenses the second part of the sample in the sample storage position 410 into the second processing position 432 with pre-prepared pre-treatment reagent for pre-treatment; then the pre-treated second part of the sample is dispensed into the second detection channel 442 of the sample test carrier 4.

[0099] The sample test carrier 4 is equipped with a second processing position 432, which can pre-process some analytes in the sample that do not require staining treatment, so as to improve the accuracy of detection.

[0100] In other embodiments, when the sample detection device detects the sample test carrier 4 with the second processing position 432, it can select whether pretreatment is required based on the detection requirements of the analyte in the sample. For example, in the detection process of some samples, if the second part of the sample (analyte detection without staining) requires pretreatment, the sample detection device first dispenses the second part of the sample into the second processing position 432 for pretreatment, and then dispenses the pretreated second part of the sample into the second detection channel 442; or, in the detection process of some samples, if the second part of the sample (analyte detection without staining) does not require pretreatment, the sample detection device directly dispenses the second part of the sample into the second detection channel 442.

[0101] Please refer to Figure 1 and Figure 8 In some embodiments, the sample test carrier 4 is supplemented with a second reagent storage position 422 based on the sample test carrier 4 having the second processing position 432 described above.

[0102] The second reagent storage compartment 422 is pre-filled with pretreatment reagents, which may include, but are not limited to, dilution reagents and separation reagents. The second reagent storage compartment 422 may also be equipped with a cap such as a film, which covers the staining reagent inside the second reagent storage compartment 422. The cap can be torn open during use, or the sampling device 11 can directly puncture the cap.

[0103] The sample test carrier 4 is provided with a second reagent storage position 422. The sample detection device has a corresponding detection mode. The method steps included in this detection mode are different from those in the above embodiments in that a pretreatment reagent dispensing step is added.

[0104] Specifically: the sampling device 11 of the sampling module 1 first dispenses the second part of the sample in the sample storage position 410 into the second processing position 432 which does not have pre-prepared reagent, and dispenses the pre-prepared reagent in the second reagent storage position 422 into the second processing position 432, and the second part of the sample is pre-treated in the second processing position 432; finally, the pre-treated second part of the sample is dispensed into the second detection channel 442 of the sample test carrier 4.

[0105] The order in which the second part of the sample is dispensed to the second processing position 432 and the pretreatment reagent is dispensed to the second processing position 432 is not limited, and the order of these two steps can be set according to actual needs.

[0106] The sample detection device adds a second reagent storage position 422, and sets the pretreatment reagent in a position other than the second processing position 432, so that the second processing position 432 can provide a place for other pretreatments and can increase the other functions of the sample test carrier 4.

[0107] In other embodiments, the sample test carrier 4 is provided with multiple reagent storage positions, such as multiple second reagent storage positions 422 pre-filled with different pretreatment reagents. Different pretreatment reagents pre-treat different samples or different analytes to meet the needs of detecting analytes in more samples.

[0108] Please refer to Figure 9 In some embodiments, the sample test carrier 4 includes only the first detection channel 441 and the second detection channel 442.

[0109] The sample test carrier 4 can dispense the stained first part of the sample into the first detection channel 441 manually or by other means outside the sample detection device, and dispense the unstained second part of the sample into the second detection channel 442.

[0110] During the sample detection process, the sample test carrier 4, which contains a stained first portion of the sample in the first detection channel 441 and an unstained second portion of the sample in the second detection channel 442, is placed inside the sample detection device. The detection module 2 of the sample detection device directly takes pictures of the stained first portion of the sample in the first detection channel 441 and the unstained second portion of the sample in the second detection channel 442. The images are then processed and analyzed to obtain the detection results.

[0111] This sample detection device can achieve semi-automatic detection, with the staining and dispensing of samples performed outside the device, which can meet the detection needs of some scenarios.

[0112] In other embodiments, when the sample test carrier 4 includes structures such as a sample storage position 410 and a first processing position 431, the staining process and the sample dispensing process can be completed manually or by other means outside the sample detection device, and the image detection and image processing can be performed directly inside the sample detection device to obtain the detection results.

[0113] Please refer to Figures 2 to 9 In some embodiments, the first detection channel 441 and the second detection channel 442 of the sample test carrier 4 can be independent of each other and have the same structure. The first detection channel 441 and the second detection channel 442 have cavities that can be used to contain liquids or suspensions. These cavities can be used to contain samples in liquid or suspension states and spread the samples flat to form a thin liquid layer for optical detection.

[0114] Please refer to Figure 9 In some embodiments, both the first detection channel 441 and the second detection channel 442 include an injection port 4411, a detection cavity 4412, and an exhaust port 4413 connected in sequence. A microfluidic channel 4414, such as a capillary channel, connecting the injection port 4411 and the detection cavity 4412 is provided between them. The microfluidic channel 4414 has a siphon effect, drawing the sample dispensed into the injection port 4411 into the detection cavity 4412. The detection cavity 4412 has a flat cavity structure, spreading the sample into a thin liquid layer. At least the upper wall of the detection cavity 4412 is transparent, allowing the detection module 2 located above the detection cavity 4412 to photograph and image the sample within the detection cavity 4412. The exhaust port 4413 is located on the side of the detection cavity 4412 away from the injection port 4411, and is used to expel air from the detection cavity 4412, allowing the sample to enter the detection cavity 4412.

[0115] In some embodiments, the sample test carrier 4 can be a transparent structure, for example, it can be made of transparent plastic material. The transparent sample test carrier 4 allows for clear viewing of the amount of sample and reagent, as well as the location of the sample and reagent, which can improve the accuracy of the test and facilitate manual operation by the operator.

[0116] Please refer to Figures 2 to 9 In some embodiments, the sample test carrier 4 is a chip-like structure of a flat plate or thin plate, and the sample storage position 410, the first processing position 431, the first detection channel 441 and the second detection channel 442 are arranged on the same side of the sample test carrier 4.

[0117] The sample storage position 410, the first reagent storage position 421, and the first processing position 431 are integrally formed groove structures on the sample test carrier 4. These groove structures form accommodating cavities to store samples or reagents, or to provide a space for sample and reagent reactions. The integrally structured sample test carrier 4 has a more accurate positional correspondence, reducing installation errors and improving detection accuracy. Furthermore, the integrally structured sample test carrier 4 has stronger structural stability, which can improve the accuracy of sample or reagent dispensing.

[0118] The sample storage position 410, the first reagent storage position 421 and the first processing position 431 can also be component structures installed on the sample test carrier 4. The sample storage position 410, the first reagent storage position 421 and the first processing position 431 are fixed on the sample test carrier 4 by means of adhesive, snap-fit ​​or other methods.

[0119] The sample testing carrier 4 can be divided into multiple areas, such as a sample placement area a, a reagent storage area b, a reaction area c, and a detection area d. The sample placement area a is provided with a sample storage position 410, the reagent storage area b is provided with a first reagent storage position 421 and a second reagent storage position 422, the reaction area c is provided with a first processing position 431 and a second processing position 432, and the detection area d is provided with a first detection channel 441 and a second detection channel 442. Figure 8 In the sample test carrier 4, the dashed lines separate the sample placement area a, the reagent storage area b, the reaction area c, and the detection area d. The dashed lines serve only as distinguishing markers, and in the actual sample test carrier 4, the dashed lines may not be present.

[0120] In other embodiments, if the sample test carrier 4 does not have structures such as the first reagent storage position 421, the second reagent storage position 422, and the second processing position 432, the sample test carrier 4 can reduce the setting of the corresponding area or reduce the area of ​​the region accordingly.

[0121] The sample placement area a, reagent storage area b, reaction area c, and detection area d are distributed sequentially along the length of the sample test carrier 4. During the detection process, the sample and reagent are also transferred and dispensed along the length of the sample test carrier 4, which can shorten the movement path of the sampling device 11 of the detection module 2 and improve the detection efficiency.

[0122] In some embodiments, a sample detection method is provided, which is implemented using the sample detection device described in the above embodiments. The sample test carrier 4 used in this sample detection method is the same as that in the above embodiments, and the sample and analyte are also the same as those in the above embodiments, so they will not be described again.

[0123] In the sample test carrier 4 corresponding to the sample detection method of this embodiment, the first processing position 431 is pre-filled with staining reagent.

[0124] Please refer to Figure 10 The sample detection method in this embodiment mainly includes the following steps:

[0125] S10: Staining treatment;

[0126] The sampling device 11 of the control sampling module 1 dispenses the first part of the sample stored in the sample storage position 410 of the sample test carrier 4 into the first processing position 431 of the sample test carrier 4.

[0127] Within the first processing position 431, the first part of the sample reacts with the pre-set staining reagent. The staining reagent stains the analyte in the first part of the sample, so that the analyte after staining can exhibit clear staining characteristics.

[0128] S20: Dispense the stained sample into the first detection channel;

[0129] The sampling device 11 of the control sampling module 1 dispenses the first part of the stained sample into the first detection channel 441 of the sample test carrier 4.

[0130] Specifically, the first part of the stained sample is injected into the injection port 4411 of the first detection channel 441 of the sample test carrier 4. The first part of the stained sample enters the detection chamber 4412 of the first detection channel 441 through the injection port 4411, and forms a thin liquid layer in the detection chamber 4412.

[0131] S30: Dispense the unstained sample into the second detection channel;

[0132] The sampling device 11 of the control sampling module 1 directly dispenses the second part of the sample into the second detection channel 442 of the sample test carrier 4 without pretreatment such as staining.

[0133] Specifically, the unstained second part of the sample is injected into the injection port 4411 of the second detection channel 442. The unstained second part of the sample enters the detection chamber 4412 of the second detection channel 442 through the injection port 4411, and forms a thin liquid layer in the detection chamber 4412.

[0134] S40: Photo capture;

[0135] The control detection module 2 takes a picture of the first part of the stained sample in the first detection channel 441 of the sample test carrier 4 to obtain the first image;

[0136] The control detection module 2 takes a picture of the unstained second part of the sample in the second detection channel 442 of the same sample test carrier 4 to obtain a second image.

[0137] The detection module 2 can take pictures of the first part of the sample and the second part of the sample sequentially, and the order of taking pictures can be interchanged. If the detection module 2 is equipped with multiple microscope detection modules, it can also take pictures of the first part of the sample and the second part of the sample simultaneously.

[0138] S50: Image processing yields detection results.

[0139] The processing module 3 acquires the first image and the second image captured by the detection module 2, processes the first image to obtain the detection result of the stained test object, and processes the information of the second image to obtain the detection result of the unstained test object.

[0140] In this sample detection method, the order of the first part of the sample being labeled and the second part of the sample being labeled is not limited; the order of these two steps can be set according to actual needs.

[0141] In the sample detection method of this embodiment, the sampling device 11 of the sampling module 1 can employ a single sample aspiration followed by two dispensing operations. Specifically, after the sampling device 11 of the sampling module 1 aspirates a sample from the sample storage location 410, it dispenses a first portion of the aspirated sample into the first processing location 431 and a second portion of the aspirated sample into the second detection channel 442. Employing a single sample aspiration followed by two dispensing operations improves the efficiency of sample dispensing, thereby increasing the detection efficiency.

[0142] In other embodiments, the sampling device 11 of the sampling module 1 can employ two sampling and two dispensing operations. Specifically, after the sampling device 11 of the sampling module 1 extracts a first portion of the sample from the sample storage location 410, it dispenses the first portion of the sample into the first processing location 431; then, the sampling device 11 of the sampling module 1 extracts a second portion of the sample from the sample storage location 410 and dispenses the extracted second portion of the sample into the second detection channel 442. Employing two sampling and two dispensing operations can also divide the sample into two parts and dispense them separately to achieve both stained and unstained detection of the sample.

[0143] In other embodiments, module 1 can be used to extract samples from other sample storage locations, such as sample racks inside or outside the sample testing device.

[0144] The sample detection method of this embodiment, through the control sampling module 1, enables the same sample within the sample test carrier 4 to be dispensed into different detection channels for detection, thus placing analytes requiring staining and those not requiring staining in different detection channels. The control detection module 2 can take pictures of the sample with stained analytes and the sample with unstained analytes respectively to obtain a first image and a second image. The processing module 3 can process the first and second images to obtain the detection results for stained and unstained analytes. The sample detection device enables simultaneous staining and unstaining of the same sample, thereby simultaneously obtaining detection results related to cells and / or bacteria (stained analytes) and detection results related to parasites and / or digestive products (unstained analytes), thus covering more detection items.

[0145] Secondly, the sample detection device can automate the detection of both stained and unstained samples, resulting in high detection efficiency. It can avoid errors caused by manual detection and prevent damage to personnel during the manual detection process.

[0146] Furthermore, this sample detection method injects the stained and unstained analytes into two independent detection channels on the sample test carrier 4 for detection, ensuring that the stained and unstained analytes are independent of each other and do not interfere with each other, thus guaranteeing the accuracy of detecting the stained and unstained analytes separately.

[0147] In some embodiments, a sample detection method is provided, the difference between this sample detection method and the above-mentioned sample detection method is that a step of dispensing staining reagent is added.

[0148] In the sample testing carrier 4 corresponding to the sample detection method of this embodiment, the first processing position 431 is not pre-filled with staining reagent. The sample testing carrier 4 is provided with a first reagent storage position 421, which is pre-filled with staining reagent.

[0149] Please refer to Figure 11 Step S10 of this sample detection method also includes the step of dispensing staining reagent. This sample detection method includes the following steps:

[0150] S11: Dispensing staining reagent;

[0151] The control sampling module 1 dispenses the staining reagent in the first reagent storage position 421 of the sample test carrier 4 into the first processing position 431 where no staining reagent is pre-filled.

[0152] S12: Staining treatment;

[0153] The control sampling module 1 dispenses the first part of the sample from the sample storage position 410 of the sample test carrier 4 into the first processing position 431 of the sample test carrier 4 where no staining reagent is pre-filled;

[0154] Within the first processing position 431, the first part of the sample reacts with the pre-set staining reagent. The staining reagent stains the analyte in the first part of the sample, so that the analyte after staining can exhibit clear staining characteristics.

[0155] Steps S20 to S50 are the same as in the above embodiment and will not be repeated.

[0156] Alternatively, the first part of the sample can be injected into the first processing position 431 first, and then the staining reagent can be injected into the first processing position 431.

[0157] In this embodiment, the sample test carrier 4 is additionally provided with a first reagent storage position 421, and the staining reagent is placed in a position other than the first processing position 431, so that the first processing position 431 can provide a place for other pretreatments other than staining, which can increase other functional uses of the sample test carrier 4.

[0158] In some embodiments, a sample detection method is provided, which differs from any of the above sample detection methods in that a second sample preprocessing step is added.

[0159] In the sample testing carrier 4 corresponding to the sample detection method in this embodiment, a second processing position 432 is added to the sample testing carrier 4, and a pre-treatment reagent is pre-placed in the second processing position 432.

[0160] The sample detection method of this embodiment further includes a second sample preprocessing step in step S30, in which the sampling device 11 of the control sampling module 1 indirectly distributes the second part of the sample to the second detection channel.

[0161] Please refer to Figure 12 and Figure 13 The sample detection method in this embodiment includes the following steps:

[0162] S31: Part Two - Sample Preprocessing;

[0163] The sampling device 11 of the control sampling module 1 first dispenses the second part of the sample in the sample storage position 410 of the sample test carrier 4 into the second processing position 432 with pre-prepared pre-treatment reagent for pre-treatment.

[0164] S32: Dispense the unstained sample into the second detection channel;

[0165] The sampling device 11 of the control sampling module 1 dispenses the pre-processed second part of the sample in the second processing position 432 into the second detection channel 442 of the sample test carrier 4.

[0166] Steps S10, S20, S40, and S50 are the same as those in the above embodiments.

[0167] In this embodiment, the sample test carrier 4 is provided with a second processing position 432, which can pre-process some analytes in the sample that do not require staining treatment, so as to improve the accuracy of detection.

[0168] In other embodiments, when testing the sample test carrier 4 with the second processing position 432, pretreatment can be selected based on the detection requirements of the analyte in the sample. For example, during the detection of some samples, if the second part of the sample (analyte detection without staining) requires pretreatment, the sample detection device first dispenses the second part of the sample into the second processing position 432 for pretreatment, and then dispenses the pretreated second part of the sample into the second detection channel 442; or, during the detection of some samples, if the second part of the sample (analyte detection without staining) does not require pretreatment, the sample detection device directly dispenses the second part of the sample into the second detection channel 442.

[0169] In some embodiments, a sample detection method is provided, which differs from the sample detection methods described above in that it adds a step of dispensing pretreatment reagents and a second part of sample pretreatment.

[0170] In the sample testing carrier 4 corresponding to the sample detection method in this embodiment, a second reagent storage position 422 and a second processing position 432 are added to the sample testing carrier 4. The second reagent storage position 422 is pre-filled with pre-treatment reagent, while the second processing position 432 is not pre-filled with pre-treatment reagent.

[0171] Please refer to Figure 14 and Figure 15 The sample detection method in this embodiment further includes a dispensing pretreatment reagent and a second part sample pretreatment step in step S30, and the sampling device 11 of the control sampling module 1 indirectly dispenses the second part of the sample into the second detection channel.

[0172] The sample detection method of this embodiment includes the following steps:

[0173] S31: Dispensing pretreatment reagent;

[0174] Control the dispensing of pretreatment reagents from the second reagent storage location 422 into the second treatment location 432;

[0175] S32: Part Two - Sample Preprocessing;

[0176] The sampling device 11 of the control sampling module 1 first dispenses the second part of the sample in the sample storage position 410 of the sample test carrier 4 into the second processing position 432 for preprocessing;

[0177] S33: Dispense the unstained sample into the second detection channel;

[0178] The sampling device 11 of the control sampling module 1 dispenses the pre-processed second part of the sample in the second processing position 432 into the second detection channel 442 of the sample test carrier 4.

[0179] Steps S10, S20, S40, and S50 are the same as those in the above embodiments.

[0180] The sample detection device adds a second reagent storage position 422, and sets the pretreatment reagent in a position other than the second processing position 432, so that the second processing position 432 can provide a place for other pretreatments and can increase the other functions of the sample test carrier 4.

[0181] Please refer to Figure 16 In some embodiments, a sample detection method is provided. This sample detection method only includes steps S40 and S50 in the above embodiments, that is, only includes the steps of taking pictures and imaging and image processing to obtain detection results in the above embodiments.

[0182] This sample detection device can achieve semi-automatic detection, with the staining and dispensing of samples performed outside the device, which can meet the detection needs of some scenarios.

[0183] The above-described specific examples are for illustrative purposes only and are not intended to limit the scope of this invention. Those skilled in the art can make various simple deductions, modifications, or substitutions based on the concept of this invention.

Claims

1. A sample detection device, characterized in that, include: The sampling module is used to inject a first portion of the sample into the first processing position of the sample test carrier for staining, and then inject the stained first portion of the sample into the first detection channel of the sample test carrier. The sampling module is also used to directly or indirectly inject the second part of the sample into the second detection channel of the sample test carrier, wherein the first part of the sample and the second part of the sample belong to the same sample. The detection module is used to take pictures of the first portion of the sample after staining in the first detection channel to obtain a first image; it is also used to take pictures of the second portion of the sample that is not stained in the second detection channel to obtain a second image. The system also includes a processing module connected to the detection module. The processing module is used to acquire the first image and the second image, process the first image to obtain the detection result of the stained test object, and process the information of the second image to obtain the detection result of the unstained test object. The first detection channel and the second detection channel are independent of each other.

2. The sample detection device as described in claim 1, characterized in that, The sampling module is used to draw up a sample and inject the first part of the drawn up sample into the first processing position of the sample test carrier for staining, and inject the second part of the drawn up sample into the second detection channel of the sample test carrier.

3. The sample detection device as described in claim 1, characterized in that, The detection module is a microscope detection module.

4. The sample detection device as described in claim 1, characterized in that, The sample detection device is used to detect a sample in at least one of the sample test carriers, including the first detection channel and the second detection channel.

5. The sample detection device according to any one of claims 1 to 4, characterized in that, It also includes a sample testing carrier, and one of the sample testing carriers includes at least the first detection channel and the second detection channel.

6. The sample detection device as described in claim 5, characterized in that, The first detection channel and the second detection channel are cavities for containing liquids or suspensions; and / or, no reagents are pre-placed in the first detection channel and the second detection channel.

7. The sample detection device as described in claim 5, characterized in that, Both the first detection channel and the second detection channel include an injection port, a detection chamber and an exhaust port connected in sequence, and at least the upper wall of the detection chamber is a transparent structure.

8. The sample detection device as described in claim 7, characterized in that, The detection cavity is a flat cavity, and the detection cavity is used to present the first part of the sample or the second part of the sample as a thin liquid layer.

9. The sample detection device as described in claim 7, characterized in that, A microfluidic channel is connected between the injection port and the detection chamber, and the microfluidic channel is used to siphon the first part of the sample or the second part of the sample into the detection chamber.

10. The sample detection device as described in claim 5, characterized in that, The sample test carrier is a disposable consumable and can be detachably installed on the sample detection device.

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