A convenient observation and operation's monorchis lily tissue culture bottle

Abstract

The utility model provides a kind of convenient observation and operation's Rhizoglyphis organ culture bottle, it is related to Rhizoglyphis organ culture field, including: culture bottle body and bottle cap, the sidewall of culture bottle body is additionally provided with convenient operation mechanism;Convenient observation mechanism, the convenient observation mechanism includes culture dish, rotating assembly and light supplement component;By being arranged convenient observation mechanism, it can be convenient to cultivate Rhizoglyphis tissue in culture bottle body according to use needs and be rotated and illuminated horizontally, to reach the purpose that it is not necessary to rotate culture bottle body and observe, effectively convenient for staff to be fully and clearly observed and recorded from the outside of culture bottle body to cultivate Rhizoglyphis tissue, by being arranged convenient operation mechanism, it can be convenient to cultivate Rhizoglyphis tissue and be maintained by bottle body directly according to use needs, to reach the purpose that it is not necessary to repeat rotating bottle cap to open or close culture bottle body and maintain operation, it is simple and convenient to operate, save time and labour, effectively improve the purpose of use convenience.

Description

Technical Field

[0001] This utility model relates to the field of *Orchid of the Garlic* tissue culture, and in particular to an *Orchid of the Garlic* tissue culture bottle that is convenient for observation and operation. Background Technology

[0002] The single-clove orchid is a rare and endangered plant belonging to the genus *Symplocos* of the Orchidaceae family. It mostly grows in high-altitude mountainous areas and has high ornamental value and certain medicinal value. Due to its low natural reproduction rate and the great impact of habitat destruction on wild populations, it is often necessary to rely on plant tissue culture technology to artificially propagate it. The culture bottle is the core container for facilitating the tissue culture of the single-clove orchid. When using the culture bottles, they are usually made of transparent glass, with a bottle body and a cap. The culture medium used to culture the *Orchidia solani* tissue is spread evenly at the bottom of the culture bottle, and then the *Orchidia solani* tissue is planted in the culture medium. Under the action of the culture medium, the *Orchidia solani* tissue gradually differentiates into a complete seedling, thus achieving the effect of tissue culture of *Orchidia solani*. However, when using the above method, it is often necessary to observe and record the tissue culture process frequently during the cultivation of *Cymbidium goeringii* tissue. The traditional method is to observe the *Cymbidium goeringii* tissue inside by holding the culture bottle and rotating it in your hand. However, when observing using this method, there are often blind spots due to the convex or concave structure of the bottle caused by the mold process, or the label on the bottle, which affects the normal observation and recording of the staff and makes it difficult to fully observe the *Cymbidium goeringii* tissue culture.

[0003] Therefore, it is necessary to provide a tissue culture flask for *Cymbidium goeringii* that is easy to observe and operate to solve the above problems. Utility Model Content

[0004] To address the technical problem of blind spots in the observation and recording of *Ormosia uniflora* tissue culture caused by protrusions or depressions in the bottle due to the molding process or by labels on the bottle, which hinders normal observation and recording by staff, this invention provides an *Ormosia uniflora* tissue culture bottle that facilitates observation and operation.

[0005] This utility model provides a convenient observation and operation orchid tissue culture bottle, comprising: a culture bottle body and a bottle cap, wherein the top of the culture bottle body and the bottle cap are detachably connected by threads, and the side wall of the culture bottle body is also provided with a convenient operation mechanism; a convenient observation mechanism, which includes a culture dish, a rotating component and a supplementary lighting component, wherein the bottom of the culture dish is installed at the bottom of the culture bottle body through the rotating component, and the supplementary lighting component is installed at the top of the culture dish.

[0006] Preferably, the rotating assembly includes a rotating support shaft, a rotating gear, and a limiting unit. The rotating support shaft is rotatably connected to the bottom center of the culture flask body through the culture flask body. The bottom center of the culture dish is fixedly connected to the top of the rotating support shaft. The rotating gear is fixedly connected to the bottom of the rotating support shaft. The limiting unit is installed at the bottom of the culture flask body.

[0007] Preferably, the limiting unit includes a fixed block, a telescopic slot, a first spring, and a limiting block. The top of the fixed block is fixedly connected to the bottom of the culture bottle. The telescopic slot is opened at one end of the fixed block. One end of the first spring is fixedly connected to one end of the telescopic slot. The side wall of the limiting block is slidably connected to the side wall of the telescopic slot. One end of the limiting block is fixedly connected to the other end of the first spring. The other end of the limiting block meshes with the side wall of the rotating gear.

[0008] Preferably, the supplemental lighting assembly includes a supplemental lighting strip and a battery box, wherein the supplemental lighting strip is fixedly connected to the top of the culture dish, and the battery box is fixedly connected to the side wall of the culture dish.

[0009] Preferably, the convenient operating mechanism includes an operating window and a telescopic opening and closing component. The operating window is located on the side wall of the culture flask, and the telescopic opening and closing component is installed on the top of the operating window.

[0010] Preferably, the telescopic opening and closing assembly includes a telescopic slide, a second spring, and an opening and closing baffle. The telescopic slide is located at the top of the operating window. One end of the second spring is fixedly connected to the top of the telescopic slide. The side wall of the opening and closing baffle is slidably connected to the side wall of the telescopic slide, and the top of the opening and closing baffle is fixedly connected to the other end of the second spring.

[0011] Compared with related technologies, the novel *Gynostemma pentaphyllum* tissue culture flask provided by this invention, which is convenient for observation and operation, has the following beneficial effects: By incorporating a convenient observation mechanism, the cultured *Ormosia uniflora* tissue within the culture bottle can be horizontally rotated and illuminated as needed. This eliminates the need to rotate the culture bottle for observation, avoiding blind spots caused by protrusions or depressions in the culture bottle due to the molding process or labels on the bottle. It effectively allows staff to fully and clearly observe and record the cultured *Ormosia uniflora* tissue from the outside of the culture bottle. Furthermore, the convenient operating mechanism allows for direct maintenance operations on the cultured *Ormosia uniflora* tissue through the bottle body as needed, eliminating the need to repeatedly rotate the cap to open or close the culture bottle for maintenance operations. This simple, convenient, time-saving, and labor-saving operation significantly improves usability. Attached Figure Description

[0012] Figure 1This is a structural schematic diagram of the present invention; Figure 2 Exploded cross-sectional view of the structure provided for this utility model; Figure 3 A partial structural bottom sectional view provided for this utility model; Figure 4 A cross-sectional view of the limiting unit provided by this utility model.

[0013] The following are the labeling elements in the diagram: 1. Culture flask body; 2. Bottle cap; 3. Convenient observation mechanism; 301. Petri dish; 4. Rotating assembly; 401. Rotating support shaft; 402. Rotating gear; 5. Limiting unit; 501. Fixing block; 502. Telescopic slot; 503. First spring; 504. Limiting block; 6. Supplemental lighting assembly; 601. Supplemental light strip; 602. Battery box; 7. Convenient operation mechanism; 701. Operation window; 8. Telescopic opening and closing assembly; 801. Telescopic slide; 802. Second spring; 803. Opening and closing baffle. Detailed Implementation

[0014] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0015] Please refer to the following: Figures 1 to 4 A tissue culture bottle for easy observation and operation of *Cymbidium goeringii* includes: a culture bottle body 1 and a bottle cap 2, wherein the top of the culture bottle body 1 and the bottle cap 2 are detachably connected by threads, and the side wall of the culture bottle body 1 is also provided with a convenient operation mechanism 7. The convenient observation mechanism 3 includes a petri dish 301, a rotating component 4, and a supplementary lighting component 6. The bottom of the petri dish 301 is installed at the bottom of the culture bottle 1 via the rotating component 4, and the supplementary lighting component 6 is installed at the top of the petri dish 301. The rotating assembly 4 includes a rotating support shaft 401, a rotating gear 402, and a limiting unit 5. The rotating support shaft 401 is rotatably connected to the bottom center of the culture flask 1 through the culture flask body 1. The bottom center of the culture dish 301 is fixedly connected to the top of the rotating support shaft 401. The rotating gear 402 is fixedly connected to the bottom of the rotating support shaft 401. The limiting unit 5 is installed at the bottom of the culture flask body 1. The limiting unit 5 includes a fixing block 501, a telescopic slot 502, a first spring 503, and a limiting block 504. The top of the fixing block 501 is fixedly connected to the bottom of the culture bottle 1. The telescopic slot 502 is opened at one end of the fixing block 501. One end of the first spring 503 is fixedly connected to one end of the telescopic slot 502. The side wall of the limiting block 504 is slidably connected to the side wall of the telescopic slot 502. One end of the limiting block 504 is fixedly connected to the other end of the first spring 503. The other end of the limiting block 504 meshes with the side wall of the rotating gear 402. The supplemental lighting assembly 6 includes a supplemental lighting strip 601 and a battery box 602. The supplemental lighting strip 601 is fixedly connected to the top of the culture dish 301, and the battery box 602 is fixedly connected to the side wall of the culture dish 301.

[0016] In the specific implementation process, firstly, the culture bottle 1 can be opened by rotating the threaded cap 2. Then, the culture medium and *Ormosia uniflora* tissue are placed on the culture dish 301. The culture bottle 1 is then closed by the cap 2, allowing the *Ormosia uniflora* tissue to be cultured within the culture bottle 1. When it is necessary to observe and record the culture process, the limiting block 504 can be slid and retracted into the telescopic slot 502 at one end of the fixing block 501. Once inserted into the telescopic slot 502, the engagement with the rotating gear 402 is released. At this point, rotating the rotating gear 402 causes the connected rotating support shaft 401 to rotate at the bottom of the culture flask 1. The rotation of the rotating support shaft 401 causes the connected culture dish 301 to rotate within the culture flask 1. Simultaneously, power is supplied by the battery in the battery box 602, activating the supplemental lighting strip 601 to emit light. The illumination of the supplemental lighting strip 601 provides illumination for the culture dish. The *Ormosia uniflora* tissue in the culture dish 301 is illuminated by supplemental lighting. During this process, the culture dish 301 rotates within the culture flask 1, and supplemental lighting is provided by the supplemental light strip 601. This allows for thorough and clear observation and recording of the *Ormosia uniflora* tissue cultured within the culture flask 1. After observation, the first spring 503 provides a reaction force, causing the limiting block 504 to spring back and extend from the telescopic slot 502. This extension of the limiting block 504 allows it to engage with the side wall of the rotating gear 402, thus enabling the rotation... The gear 402 is used for limiting and fixing, which in turn limits and fixes the culture dish 301 and the cultivated *Hylocereus undatus* tissue inside. This allows for easy horizontal rotation and illumination of the cultivated *Hylocereus undatus* tissue inside the culture bottle 1 as needed, without having to rotate the culture bottle 1 for observation. This avoids situations where the culture bottle 1 has a raised or recessed structure due to the mold process, or where the label on the bottle body obstructs the view and causes blind spots. It effectively facilitates the staff to fully and clearly observe and record the cultivated *Hylocereus undatus* tissue from the outside of the culture bottle 1. Furthermore, the convenient operating mechanism 7 includes an operating window 701 and a telescopic opening and closing component 8. The operating window 701 is opened on the side wall of the culture bottle body 1, and the telescopic opening and closing component 8 is installed on the top of the operating window 701. The telescopic opening and closing assembly 8 includes a telescopic slide 801, a second spring 802, and an opening and closing baffle 803. The telescopic slide 801 is opened at the top of the operation window 701. One end of the second spring 802 is fixedly connected to the top of the telescopic slide 801. The side wall of the opening and closing baffle 803 is slidably connected to the side wall of the telescopic slide 801, and the top of the opening and closing baffle 803 is fixedly connected to the other end of the second spring 802.

[0017] Based on the above embodiments, when it is necessary to perform maintenance operations on the cultured *Ormosia uniflora* tissue, the opening and closing baffle 803 can be pulled upwards to slide and retract into the telescopic groove 801 at the top of the operation window 701. The operation window 701 can then be opened by retracting the baffle 803 into the telescopic groove 801. At this time, the open operation window 701 allows staff to conveniently use tools to directly perform maintenance operations on the *Ormosia uniflora* tissue cultured in the culture dish 301, and the operation can be completed afterward. The second spring 802 provides a reaction force, which causes the opening and closing baffle 803 to spring back and extend from the telescopic slide 801 and fall down. The opening and closing baffle 803 can block the operation window 701, so that the cultured garlic orchid tissue can be directly maintained according to the needs of use. This eliminates the need to repeatedly rotate the bottle cap 2 to open or close the culture bottle 1 for maintenance. The operation is simple, convenient, time-saving and labor-saving, and effectively improves the ease of use. It should be noted that, for normal observation, the culture bottle 1, culture dish 301, and opening / closing baffle 803 are all made of transparent glass. The specific components can be freely adjusted according to the actual application scenario.

[0018] The working principle of the *Ormosia uniflora* tissue culture flask provided by this utility model, which is convenient for observation and operation, is as follows: In use, firstly, the culture bottle 1 can be opened by rotating the screw-on cap 2. Then, the culture medium and *Hylocereus undatus* tissue are placed on the culture dish 301. The culture bottle 1 is then closed by turning the cap 2, allowing the *Hylocereus undatus* tissue to be cultured inside the culture bottle 1. When it is necessary to observe and record the culture process, the sliding limit block 504 can be slid into the telescopic slot 502 at one end of the fixed block 501. The retraction of the limit block 504 into the telescopic slot 502 releases its engagement with the rotating gear 402. Rotating the rotating gear 402 then causes the connected rotating support shaft 401 to rotate at the bottom of the culture bottle 1. Rotation causes the connected culture dish 301 to rotate within the culture bottle 1. Simultaneously, power is supplied by the battery in the battery box 602, activating the supplemental lighting strip 601 to illuminate the *Ormosia uniflora* tissue within the culture dish 301. The rotation of the culture dish 301 within the culture bottle 1 and the illumination from the supplemental lighting strip 601 allow for thorough and clear observation and recording of the *Ormosia uniflora* tissue. After observation, the first spring 503 provides a reaction force, causing the limiting block 504 to spring back out of the telescopic slot 502. This allows it to mesh with the side wall of the rotating gear 402, thus limiting and fixing the rotating gear 402, and consequently limiting and fixing the culture dish 301 and the cultivated *Hylocereus undatus* tissue inside. This effectively facilitates horizontal rotation and illumination of the cultivated *Hylocereus undatus* tissue inside the culture bottle 1 as needed, eliminating the need to rotate the culture bottle 1 for observation. It avoids blind spots caused by protrusions or depressions in the culture bottle 1 due to the molding process, or by labels obstructing the view. This effectively allows staff to fully and clearly observe and record the cultivated *Hylocereus undatus* tissue from the outside of the culture bottle 1. Furthermore, when maintenance operations are required on the cultivated *Hylocereus undatus* tissue, the opening and closing baffle 803 can be pulled up... The device can be slidably retracted into the telescopic groove 801 at the top of the operation window 701. The opening and closing baffle 803 retracts into the telescopic groove 801, thus opening the operation window 701. At this time, the staff can conveniently use tools to directly cultivate the *Ormosia uniflora* tissue in the culture dish 301 through the open operation window 701. After the operation is completed, the second spring 802 provides a reaction force, causing the opening and closing baffle 803 to spring back and extend from the telescopic groove 801, thus sealing the operation window 701. This effectively facilitates direct cultivation of the *Ormosia uniflora* tissue through the bottle as needed.This eliminates the need to repeatedly rotate the cap 2 to open or close the culture bottle 1 for maintenance operations, making the operation simple, convenient, time-saving, and labor-saving, effectively improving ease of use.

[0019] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A tissue culture flask for *Cymbidium goeringii* that is convenient for observation and operation, characterized in that, include: Culture bottle body (1) and bottle cap (2), wherein the top of the culture bottle body (1) and the bottle cap (2) are detachably connected by threads, and the side wall of the culture bottle body (1) is also provided with a convenient operation mechanism (7). The convenient observation mechanism (3) includes a petri dish (301), a rotating component (4) and a supplementary lighting component (6). The bottom of the petri dish (301) is installed on the bottom of the culture bottle (1) via the rotating component (4), and the supplementary lighting component (6) is installed on the top of the petri dish (301).

2. The *Gynostemma pentaphyllum* tissue culture flask for convenient observation and operation according to claim 1, characterized in that, The rotating assembly (4) includes a rotating support shaft (401), a rotating gear (402), and a limiting unit (5). The rotating support shaft (401) is rotatably connected to the bottom center of the culture bottle (1) through the culture bottle body (1). The bottom center of the culture dish (301) is fixedly connected to the top of the rotating support shaft (401). The rotating gear (402) is fixedly connected to the bottom of the rotating support shaft (401). The limiting unit (5) is installed at the bottom of the culture bottle body (1).

3. The *Gynostemma pentaphyllum* tissue culture flask according to claim 2, characterized in that, The limiting unit (5) includes a fixing block (501), a telescopic slot (502), a first spring (503), and a limiting block (504). The top of the fixing block (501) is fixedly connected to the bottom of the culture bottle (1). The telescopic slot (502) is opened at one end of the fixing block (501). One end of the first spring (503) is fixedly connected to one end of the telescopic slot (502). The side wall of the limiting block (504) is slidably connected to the side wall of the telescopic slot (502). One end of the limiting block (504) is fixedly connected to the other end of the first spring (503). The other end of the limiting block (504) meshes with the side wall of the rotating gear (402).

4. The *Gynostemma pentaphyllum* tissue culture flask according to claim 1, characterized in that, The supplemental lighting component (6) includes a supplemental lighting strip (601) and a battery box (602). The supplemental lighting strip (601) is fixedly connected to the top of the petri dish (301), and the battery box (602) is fixedly connected to the side wall of the petri dish (301).

5. The *Gynostemma pentaphyllum* tissue culture flask according to claim 1, characterized in that, The convenient operating mechanism (7) includes an operating window (701) and a telescopic opening and closing component (8). The operating window (701) is opened on the side wall of the culture bottle (1), and the telescopic opening and closing component (8) is installed on the top of the operating window (701).

6. The *Gynostemma pentaphyllum* tissue culture flask according to claim 5, characterized in that, The telescopic opening and closing assembly (8) includes a telescopic slide (801), a second spring (802), and an opening and closing baffle (803). The telescopic slide (801) is located at the top of the operation window (701). One end of the second spring (802) is fixedly connected to the top of the telescopic slide (801). The side wall of the opening and closing baffle (803) is slidably connected to the side wall of the telescopic slide (801), and the top of the opening and closing baffle (803) is fixedly connected to the other end of the second spring (802).

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