A tissue liquid nitrogen snap freezing device

Abstract

The utility model relates to a frozen storage device technical field especially, relates to a kind of tissue liquid nitrogen quick-freezing device, including heat preservation box, it is characterized by: heat preservation box is provided with bearing cup and fixed cup holder, bearing cup is fixed in the inboard of fixed cup holder, heat preservation box inner wall is equipped with fixed groove and placement groove, fixed groove is adapted with fixed cup holder, sample placement spoon is arranged in placement groove, the upper portion of heat preservation box is connected with sealing cover, heat preservation box is used to bear liquid nitrogen, bearing cup is used to bear isopentane and tissue sample.The application is well integrated with isopentane treatment and liquid nitrogen treatment, at the same time, avoid freezing injury when experimental personnel contact heat preservation box outer wall, and liquid nitrogen volatilization speed can be reduced, in addition, the protection design of first temperature-insulating sleeve and second temperature-insulating sleeve ensures that experimental personnel will not freeze when operating, significantly improves operation safety and convenient form, accurately protects sample quality, guarantees subsequent experiment reliability.

Description

Technical Field

[0001] This utility model relates to the field of cryopreservation equipment technology, and in particular to a tissue liquid nitrogen quick-freezing device. Background Technology

[0002] Modern neuroscience research is rapidly advancing, and brain tissue analysis techniques are constantly improving, including but not limited to histochemical staining and tissue fluorescence staining. However, brain tissue is an energy-intensive tissue and is also highly prone to liquefaction. Therefore, preparing qualified samples is one of the key prerequisites for ensuring the quality of subsequent tissue staining analysis results. Currently, one common method for processing high-quality brain tissue samples is rapid cryopreservation after tissue dissociation, using liquid nitrogen (at atmospheric pressure, liquid nitrogen has a temperature of -196°C) to quickly cool the sample and protect its antigenic activity. However, direct liquid nitrogen freezing can easily lead to tissue cracking. Therefore, isopentane (which can reach approximately -150°C when cooled in liquid nitrogen) is usually placed in liquid nitrogen, and then the tissue sample is placed in the isopentane for rapid freezing. This process carries safety risks, and currently, there is no integrated freezing device that combines liquid nitrogen and isopentane, ensuring both safety and ease of operation. Utility Model Content

[0003] In view of the problems mentioned in the background art, the purpose of this utility model is to provide a liquid nitrogen quick-freezing device for tissues (i.e., a liquid nitrogen quick-freezing device for tissues).

[0004] The present invention adopts the following technical solution.

[0005] A liquid nitrogen quick-freezing device for tissues includes an insulated box, characterized in that: a carrier cup and a fixed cup holder are provided inside the insulated box, the carrier cup is fixed inside the fixed cup holder, a fixing groove and a placement groove are provided on the inner wall of the insulated box, the fixing groove is adapted to the fixed cup holder, a sample placement spoon is provided in the placement groove, and a sealing cover is connected to the top of the insulated box. The insulated box is used to hold liquid nitrogen, and the carrier cup is used to hold isopentane and tissue samples.

[0006] Preferably, the cup is made of stainless steel and the insulated box is made of polycarbonate.

[0007] To improve ease of operation, a handle is connected to the outside of the cup, and the outer surface of the handle is covered with a first heat insulation sleeve. A spout is provided at the top of the cup, and scale lines are provided on the inner wall of the cup.

[0008] To improve the stability of the cup when it is fixed, the cup holder is composed of a skeleton rod and a fitting ring. The skeleton rod is a series of staggered U-shaped bent rods. The fitting ring is connected to the skeleton rod, and the inner wall of the fitting ring is in contact with the outer surface of the cup.

[0009] Preferably, a hanging rod is connected above the cup holder. The hanging rod is a bent rod, one end of which can be inserted into the fixing groove, and the bent part of the hanging rod is located on the upper surface of the fixing groove.

[0010] To facilitate the removal of the fixed cup holder while ensuring the safety of the experimenters, a fixing block is connected to one end of the hanging rod that is inserted into the fixing slot. A lifting handle is connected above the fixing block, and a second heat insulation sleeve is connected to the outer surface of the lifting handle.

[0011] To facilitate the removal of the sealing cap and improve the convenience of carrying the liquid nitrogen quick-freezing device for tissues, a lifting rope is connected to the top of the sealing cap, and portable ropes are provided on both sides of the sealing cap. The portable ropes pass through the side walls of the sealing cap and the insulated box, and both ends of the portable ropes have knots larger than their own cross-sectional diameter.

[0012] To facilitate operation by experimenters, one end of the sample placement spoon is flat and has a concave surface. The side of the sample placement spoon is marked with scale lines.

[0013] Beneficial effects: This application not only reduces the risk of low-temperature contact, but also avoids liquid nitrogen leakage and tipping of the carrier cup; more importantly, the tissue liquid nitrogen quick-freezing device of this application can avoid direct contact between tissue and liquid nitrogen, ensuring freezing uniformity, while the flat sample placement spoon with concave shape can achieve fine transfer of samples. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a three-dimensional structural diagram of the tissue liquid nitrogen quick-freezing device of this utility model.

[0016] Figure 2 This is a three-dimensional structural diagram of the tissue liquid nitrogen quick-freezing device of this utility model with the sealing cap removed.

[0017] Figure 3 This is a three-dimensional structural diagram of the support cup of the liquid nitrogen quick-freezing device for tissues according to this utility model.

[0018] Figure 4 This is a three-dimensional structural diagram of the fixed cup holder of the liquid nitrogen quick-freezing device for tissues according to this utility model.

[0019] Figure 5This is a three-dimensional structural diagram of the sample placement spoon of the liquid nitrogen quick-freezing device for tissues according to this utility model.

[0020] 1-Insulated box, 2-Bearing cup, 3-Fixed cup holder, 4-Fixed groove, 5-Placement groove, 6-Sample placement spoon, 7-Sealing cap, 8-Handle, 9-First insulation sleeve, 10-Cup spout, 11-Cup body graduation line, 12-Skeleton rod, 13-Fitting ring, 14-Hanging rod, 15-Fixing block, 16-Lifting handle, 17-Second insulation sleeve, 18-Lid lifting rope, 19-Portable rope, 20-Knot, 21-Spoon body graduation line. Detailed Implementation

[0021] The technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.

[0022] Combination Figures 1-5 As shown, a tissue liquid nitrogen quick-freezing device includes an insulated box 1, characterized in that: a carrier cup 2 and a fixed cup holder 3 are provided inside the insulated box 1, the carrier cup 2 is fixed inside the fixed cup holder 3, a fixing groove 4 and a placement groove 5 are provided on the inner wall of the insulated box 1, the fixing groove 4 is adapted to the fixed cup holder 3, a sample placement spoon 6 is provided in the placement groove 5, and a sealing cover 7 is connected to the top of the insulated box 1. The insulated box 1 is used to hold liquid nitrogen, and the carrier cup 2 is used to hold isopentane and tissue samples.

[0023] The cup 2 is made of stainless steel, and the insulated box 1 is made of polycarbonate.

[0024] The outer side of the carrying cup 2 is connected to a handle 8, the outer surface of the handle 8 is covered with a first heat insulation sleeve 9, the upper end of the carrying cup 2 is provided with a spout 10, and the inner wall of the carrying cup 2 is provided with cup body scale lines 11.

[0025] The fixed cup holder 3 is composed of a skeleton rod 12 and a fitting ring 13. The skeleton rod 12 is a plurality of staggered U-shaped bent rods. The fitting ring 13 is connected to the skeleton rod 12, and the inner wall of the fitting ring 13 is fitted to the outer surface of the cup 2.

[0026] The cup holder 3 is connected to a hanging rod 14 above it. The hanging rod 14 is a bent rod. One end of the hanging rod 14 can be inserted into the fixing groove 4, and the bent part of the hanging rod 14 is located on the upper surface of the fixing groove 4.

[0027] The ear rod 14 is inserted into the fixing groove 4 and is connected to a fixing block 15. A lifting handle 16 is connected above the fixing block 15, and a second heat insulation sleeve 17 is connected to the outer surface of the lifting handle 16.

[0028] The sealing cover 7 is connected to the top of the cover lifting rope 18, and the sealing cover 7 is provided with a portable rope 19 on both sides. The portable rope 19 passes through the sealing cover 7 and the side wall of the insulated box 1, and both ends of the portable rope 19 have a knot 20 larger than its own cross-sectional diameter.

[0029] The sample placement spoon 6 has a flat end with a concave surface, and a spoon body scale line 21 is provided on one side of the sample placement spoon 6.

[0030] When using, hold the heat-insulating handle 8 on the outside of the carrier cup 2 and pour isopentane into the cup according to the scale line 11 on the inner wall of the carrier cup 2. After adding isopentane, put the carrier cup 2 containing isopentane back into the fitting ring 13 of the fixed cup holder 3, inject liquid nitrogen into the heat preservation box 1, and close the sealing cover 7 of the heat preservation box 1. Let it stand for 15-20 minutes until the temperature of isopentane drops to -150℃~-190℃ (a low temperature thermometer can be used for auxiliary monitoring, or the temperature can be judged based on the stable state of liquid nitrogen volatilization).

[0031] After adding isopentane and pre-cooling, open the sealing cap 7 and remove the sample placement spoon 6 from the placement slot 5. Gently lift the tissue sample (such as brain tissue) with its flat, concave end to avoid squeezing or touching the sample and causing structural damage. The scale lines 21 on the side of the spoon 6 can be used to estimate the depth to which the sample is submerged in isopentane. Insert the sample placement spoon 6 into the carrier cup 2 and slowly place the tissue sample into the isopentane, ensuring the sample is completely submerged and does not float or touch the cup wall. Replace the sealing cap 7 and set the quick-freezing time according to the sample type.

[0032] After quick-freezing, hold the second insulating sleeve 17 outside the lifting handle 16 and remove the fixed cup holder 3 along with the carrier cup 2 from the insulated box 1. Pour isopentane through the spout 10 at the top of the carrier cup 2. Then, hold the first insulating sleeve 9 outside the handle 8 and use the sample placement spoon 6 to remove the frozen tissue sample, transferring it to a low-temperature storage container or directly for subsequent experiments. Clean, disinfect, and dry the carrier cup 2 and sample placement spoon 6, then return them to their corresponding positions in the apparatus. Empty the remaining liquid nitrogen from the insulated box 1 and close the sealing cap 7 for later use.

[0033] The assembly design of the fixed slot 4 and the fixed cup holder 3 enables the rapid positioning and assembly of the internal components of the device. The lifting handle 16, the lid lifting rope 18 and the carrying rope 19 facilitate the lifting and placing of components, the opening of the sealing cover 7 and the carrying of the entire device. The design of the sample placement spoon 6 and the cup spout 10 of the carrier cup 2 built into the placement slot 5 also facilitates the use of tools and subsequent cleaning, improving the overall convenience of the device.

[0034] In summary, the tissue liquid nitrogen rapid freezing device of this application integrates isopentane treatment and liquid nitrogen treatment in a good way, avoiding direct contact between tissue and liquid nitrogen, ensuring freezing uniformity, while accurately protecting sample quality, improving operational safety, optimizing ease of use, and having strong structural adaptability.

[0035] The above-disclosed embodiments are merely preferred embodiments of the present utility model and should not be construed as limiting the scope of the present utility model. Those skilled in the art can understand that implementing all or part of the above-described embodiments and making equivalent changes in accordance with the claims of the present utility model are still within the scope of the utility model.

Claims

1. A tissue liquid nitrogen quick-freezing device, comprising an insulated box (1), characterized in that: The insulated box (1) is equipped with a carrying cup (2) and a fixed cup holder (3). The carrying cup (2) is fixed inside the fixed cup holder (3). The inner wall of the insulated box (1) is provided with a fixing groove (4) and a placement groove (5). The fixing groove (4) is adapted to the fixed cup holder (3). A sample placement spoon (6) is provided in the placement groove (5). A sealing cover (7) is connected to the top of the insulated box (1). The insulated box (1) is used to hold liquid nitrogen, and the carrying cup (2) is used to hold isopentane and tissue samples.

2. The tissue liquid nitrogen quick-freezing apparatus as described in claim 1, characterized in that: The container (2) is made of stainless steel, and the insulated box (1) is made of polycarbonate.

3. The tissue liquid nitrogen quick-freezing apparatus as described in claim 1, characterized in that: A handle (8) is connected to the outside of the carrying cup (2). The outer surface of the handle (8) is covered with a first heat insulation sleeve (9). A spout (10) is provided at the upper end of the carrying cup (2). The inner wall of the carrying cup (2) is provided with cup body scale lines (11).

4. The tissue liquid nitrogen quick-freezing apparatus as described in claim 1, characterized in that: The fixed cup holder (3) is composed of a skeleton rod (12) and a fitting ring (13). The skeleton rod (12) consists of multiple staggered U-shaped bent rods. The fitting ring (13) is connected to the skeleton rod (12). The inner wall of the fitting ring (13) is fitted to the outer surface of the cup (2).

5. The tissue liquid nitrogen quick-freezing apparatus as described in claim 1, characterized in that: A hanging rod (14) is connected above the fixed cup holder (3). The hanging rod (14) is a bent rod. One end of the hanging rod (14) can be inserted into the fixed groove (4), and the bent part of the hanging rod (14) is located on the upper surface of the fixed groove (4).

6. The tissue liquid nitrogen quick-freezing apparatus as described in claim 5, characterized in that: The end of the hanging rod (14) that is inserted into the fixing groove (4) is connected to a fixing block (15), and a lifting handle (16) is connected above the fixing block (15). A second heat insulation sleeve (17) is connected to the outer surface of the lifting handle (16).

7. The tissue liquid nitrogen quick-freezing apparatus as described in claim 1, characterized in that: A lid lifting rope (18) is connected to the top of the sealing cover (7), and a portable rope (19) is provided on both sides of the sealing cover (7). The portable rope (19) passes through the side wall of the sealing cover (7) and the heat preservation box (1), and both ends of the portable rope (19) have a knot (20) larger than its own cross-sectional diameter.

8. The tissue liquid nitrogen quick-freezing apparatus as described in claim 1, characterized in that: One end of the sample placement spoon (6) is flat and has a concave surface. A spoon body scale line (21) is provided on one side of the sample placement spoon (6).

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