A centrifugal device for exosome isolation

Abstract

The application relates to a centrifugal device for exosome separation, which comprises a shell, a centrifugal rotor which is rotationally connected in the shell, the surface of the centrifugal rotor is recessed inward, and a recess is formed, a centrifugal tube which is located in the recess and closely contacts the recess, and a limiting structure which is arranged on the centrifugal rotor and comprises a restraint block which abuts against the centrifugal tube on the side of the recess recessed direction. The application sets the recess in the centrifugal rotor, so that the centrifugal tube is attached in the recess, and the centrifugal tube is fixed in the recess through the restraint block, so that the centrifugal tube can rotate synchronously with the centrifugal rotor, and the centrifugal tube is attached to the centrifugal rotor in a large area, thereby reducing the shaking of the centrifugal tube. Therefore, the application reduces the shaking of the centrifugal tube in the centrifugal process through the recess and the restraint block.

Description

Technical Field

[0001] This application relates to the technical field of tumor screening, and in particular to a centrifuge device for exosome separation. Background Technology

[0002] In the screening of urinary system tumors such as bladder cancer and kidney cancer, urine needs to be collected to isolate exosomes. Exosomes are small membrane vesicles containing complex RNA and proteins. Combined with RNA sequencing analysis of transcription profiles, disease biomarkers can be analyzed with high sensitivity. The stability of the centrifugation device directly affects the separation effect. If the centrifuge tube shakes or shifts during centrifugation, it will not only lead to uneven distribution of centrifugal force, affecting the purity and recovery rate of exosomes, but may also cause contamination or loss due to sample splashing. Especially for micro-sized biological samples, such errors may directly lead to experimental failure.

[0003] Traditional centrifuges for exosome separation rely on the weight of the centrifuge tubes or simple snap-fit ​​structures to fix them, which has limited fixing strength. During the equipment startup phase, as the centrifuge rotor accelerates from a standstill to the set speed, the centrifugal force on the centrifuge tubes changes dynamically. The unstable impact force can easily cause the centrifuge tubes to sway slightly in the fixing tank. This swaying can cause resonance when multiple tubes are centrifuged at the same time, which aggravates rotor vibration. This not only affects the centrifugation accuracy but may also shorten the service life of the equipment due to long-term high-frequency vibration. Summary of the Invention

[0004] This application provides a centrifuge device for exosome separation to solve the problem of rotor vibration caused by improperly fixed centrifuge tubes in related technologies.

[0005] In a first aspect, a centrifuge apparatus for exosome separation is provided, comprising:

[0006] case;

[0007] Centrifugal rotor, the centrifugal rotor is rotatably connected to the housing, and the surface of the centrifugal rotor is recessed into it to form a groove;

[0008] Centrifuge tubes are located inside the groove, and the centrifuge tubes are in close contact with the groove.

[0009] A limiting structure is provided on the centrifugal rotor. The limiting structure includes a constraint block, and the side of the constraint block facing the recessed groove abuts against the centrifugal tube.

[0010] In some embodiments, the groove is in close contact with the wall of the centrifuge tube, and the constraint block abuts against the opening of the centrifuge tube.

[0011] In some embodiments, there are several grooves, and each groove is equidistant from the center of the centrifugal rotor.

[0012] In some embodiments, the limiting structure also includes a connecting cover and a spring;

[0013] The two ends of the spring are fixedly connected to the connecting cover and the constraint block, respectively, and the connecting cover is connected to the centrifugal rotor.

[0014] In some embodiments, the limiting structure further includes a rotating stud;

[0015] The rotating stud engages with the centrifugal rotor, with one end of the rotating stud abutting against the constraint block, and the rotating stud positioned at the center of the centrifugal rotor.

[0016] In some embodiments, a limiting groove is provided on the top of the centrifugal rotor;

[0017] The groove and the constraint block are both located in the limiting groove, and the connecting cover is threadedly connected to the centrifugal rotor at the opening of the limiting groove.

[0018] In some embodiments, the housing is provided with a placement slot, and the centrifugal rotor is located in the placement slot.

[0019] This application provides a centrifuge device for exosome separation. Because the centrifuge rotor is provided with a groove, the centrifuge tube fits into the groove and is fixed in the groove by a constraint block. This allows the centrifuge tube to rotate synchronously with the centrifuge rotor. Since the centrifuge tube fits into the centrifuge rotor over a large area, the shaking of the centrifuge tube is reduced. Therefore, this application reduces the shaking of the centrifuge tube during the centrifugation process by using the groove and the constraint block. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the structure provided for an embodiment of this application;

[0022] Figure 2 A structural cross-sectional view provided for an embodiment of this application;

[0023] Figure 3 A cross-sectional view of the centrifugal rotor is provided for the embodiments of this application;

[0024] Figure 4 A schematic diagram of the centrifugal rotor is provided for the embodiments of this application;

[0025] Figure 5 A schematic diagram of the centrifuge tube structure is provided for the embodiments of this application;

[0026] Figure 6A schematic diagram of the constraint block is provided for the embodiments of this application;

[0027] Figure 7 A schematic diagram of the connecting cover is provided for an embodiment of this application.

[0028] In the diagram: 1. Shell; 2. Centrifugal rotor; 3. Groove; 4. Centrifuge tube; 5. Limiting structure; 51. Constraint block; 52. Connecting cover; 53. Spring; 54. Rotating stud; 6. Limiting groove; 7. Placement groove. Detailed Implementation

[0029] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present invention.

[0030] This application provides a centrifuge device for exosome separation, which can solve the problem of rotor vibration caused by improper fixation of centrifuge tubes in related technologies.

[0031] Please see Figures 1 to 7 A centrifuge device for exosome separation includes: a shell 1, a centrifuge rotor 2, a centrifuge tube 4, and a limiting structure 5. The centrifuge rotor 2 is rotatably connected to the shell 1, and the surface of the centrifuge rotor 2 is recessed inward to form a groove 3. The centrifuge tube 4 is located in the groove 3 and is in close contact with the groove 3. The limiting structure 5 is disposed on the centrifuge rotor 2 and includes a constraint block 51. The side of the constraint block 51 facing the recess of the groove 3 abuts against the centrifuge tube 4. The shell 1 is provided with a placement groove 7, and the centrifuge rotor 2 is located in the placement groove 7.

[0032] In this embodiment, a motor is installed in the housing 1, and the output end of the motor is connected to the centrifugal rotor 2. The motor drives the centrifugal rotor 2 to rotate, and the centrifuge tube 4 rotates inside the centrifugal rotor 2, thereby realizing the separation of exosomes. In actual use, the exosomes need to be loaded into the centrifuge tube 4 first, and then the exosomes are sealed in the centrifuge tube 4 with a cap. Then, the centrifuge tube 4 containing the exosomes is placed into the groove 3. After that, the constraint block 51 is moved to hold the centrifuge tube 4 against it, so that the centrifuge tube 4 can always be in contact with the groove 3. When the centrifuge tube 4 can be in contact with the centrifugal rotor 2 as much as possible, the centrifuge tube 4 will shake as little as possible when the centrifugal rotor 2 rotates, avoiding the problem of uneven force caused by the traditional single fixing method. Especially during the centrifuge start-up acceleration stage, it can effectively suppress the shaking of the centrifuge tube 4, ensure that the centrifugal force is applied evenly to the sample, and improve the purity and recovery rate of exosome separation.

[0033] In a preferred embodiment, the groove 3 is tightly fitted to the wall of the centrifuge tube 4, and the constraint block 51 abuts against the opening of the centrifuge tube 4. To ensure a tighter fit between the centrifuge tube 4 and the centrifuge rotor 2, the groove 3 is structured as follows: Figure 3 As shown, when centrifuge tube 4 is inserted into groove 3, the tube wall of centrifuge tube 4 will be tightly attached to groove 3, and the position of centrifuge tube 4 will be restricted by groove 3. In order to prevent centrifuge tube 4 from sliding out of groove 3, constraint block 51 is used to hold the opening of centrifuge tube 4.

[0034] Furthermore, in this embodiment, the number of grooves 3 is several, and the distance between each groove 3 and the center of the centrifugal rotor 2 is the same. The centrifugal rotor 2 is as follows: Figure 2 The diagram shows a platform-shaped structure. The motor output is connected to the center of the bottom of the centrifugal rotor 2. Multiple grooves 3 are arranged in a ring inside the centrifugal rotor 2, enabling the separation of multiple exosomes within one centrifugal rotor 2. When the distance between the groove 3 and the center of the centrifugal rotor 2 is the same, the separation effect of exosomes in the centrifuge tube 4 within each groove 3 is similar, making it more convenient for operators to operate.

[0035] Furthermore, in this embodiment, the limiting structure 5 also includes a connecting cover 52 and a spring 53. The two ends of the spring 53 are respectively fixedly connected to the connecting cover 52 and the constraint block 51. The connecting cover 52 is connected to the centrifugal rotor 2 and is connected to the housing 1 by threads. After the centrifugal tube 4 is placed in the groove 3, the connecting cover 52 is placed on the housing 1 and rotated to fix it to the housing 1. During the fixing process of the connecting cover 52, the constraint block 51 will go from contacting the opening of the centrifugal tube 4 to abutting the opening. The spring 53 gradually tightens and provides a force to the constraint block 51 to squeeze the centrifugal tube 4, ensuring that the centrifugal tube 4 is tightly attached to the groove 3.

[0036] In a preferred embodiment, the limiting structure 5 further includes a rotating stud 54, which cooperates with the centrifugal rotor 2. One end of the rotating stud 54 abuts against the constraint block 51, and the rotating stud 54 is located at the center of the centrifugal rotor 2. To ensure that the constraint block 51 can abut against the centrifuge tube 4, the rotating stud 54 is rotated so that it can move up and down on the connecting cover 52. When the rotating stud 54 abuts against the constraint block 51, the rotating stud 54 continues to rotate and descend, which will push the constraint block 51 downward, thereby ensuring that the constraint block 51 abuts against the centrifuge tube 4. At this time, the spring 53 pulls the constraint block 51 and continuously provides an upward force to the constraint block 51. Within the elastic range of the spring 53, the constraint block 51 will fit against the rotating stud 54, and when the rotating stud 54 rises, the constraint block 51 will rise with the rotating stud 54. The spring 53 has more functions and can ensure that the constraint block 51 abuts against the centrifuge tube 4.

[0037] Specifically, in this embodiment, a limiting groove 6 is formed on the top of the centrifugal rotor 2, and the groove 3 and the constraint block 51 are all located within the limiting groove 6. The connecting cover 52 is threadedly connected to the centrifugal rotor 2 at the opening of the limiting groove. In order to reduce the space occupied by the centrifugal rotor 2 in the housing 1, a limiting groove is formed on the top of the centrifugal rotor 2, and then the limiting structure 5 and the groove 3 are both set in the limiting groove. The structure of the limiting groove is as follows: Figure 3 As shown.

[0038] It is important to know that after the centrifuge tube 4 is placed into the groove 3, the connecting cap 52 is fixed to the centrifuge rotor 2 by threads. Then, the stud 54 is rotated, and the constraint block 51 is raised and lowered by rotating the stud 54, so that the constraint block 51 abuts against the centrifuge tube 4. Due to the self-locking function of the threads, the position of the constraint block 51 will not change, and thus the position of the centrifuge tube 4 in the groove 3 will not change. The centrifuge tube 4 will always be attached to the centrifuge rotor 2. After the centrifuge tube 4 completes the exosome separation, the connecting cap 52 is rotated to separate the connecting cap 52 from the centrifuge rotor 2, and then the centrifuge tube 4 is taken out from the groove 3.

[0039] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered illustrative and non-limiting in all respects. The scope of the invention is defined by the appended claims rather than the foregoing description, and thus all variations falling within the meaning and scope of the equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0040] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A centrifuge apparatus for exosome separation, characterized in that: case; A centrifugal rotor, which is rotatably connected to the housing, has its surface recessed inward to form a groove; Centrifuge tube, wherein the centrifuge tube is located within the groove and is in close contact with the groove; A limiting structure is provided on the centrifugal rotor. The limiting structure includes a constraint block, and the side of the constraint block facing the recessed direction of the groove abuts against the centrifugal tube.

2. The centrifuge apparatus for exosome separation according to claim 1, characterized in that: The groove is in close contact with the wall of the centrifuge tube, and the constraint block abuts against the opening of the centrifuge tube.

3. The centrifuge apparatus for exosome separation according to claim 1, characterized in that: The number of grooves is several, and each groove is equidistant from the center of the centrifugal rotor.

4. The centrifuge apparatus for exosome separation according to claim 1, characterized in that: The constraint block has a frustum structure, and the groove is inclinedly disposed inside the centrifugal rotor.

5. The centrifuge apparatus for exosome separation according to claim 1, characterized in that: The limiting structure also includes a connecting cover and a spring; The two ends of the spring are respectively fixedly connected to the connecting cover and the constraint block, and the connecting cover is connected to the centrifugal rotor.

6. The centrifuge apparatus for exosome separation according to claim 5, characterized in that: The limiting structure also includes a rotating stud; The rotating stud engages with the centrifugal rotor, with one end of the rotating stud abutting against the constraint block, and the rotating stud located at the center of the centrifugal rotor.

7. The centrifuge apparatus for exosome separation according to claim 5, characterized in that: A limiting groove is provided on the top of the centrifugal rotor; Both the groove and the constraint block are located within the limiting groove, and the connecting cover is threadedly connected to the centrifugal rotor at the opening of the limiting groove.

8. The centrifuge apparatus for exosome separation according to claim 1, characterized in that: The housing is provided with a placement groove, and the centrifugal rotor is located in the placement groove.

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