High efficiency polypeptide extraction centrifuge

Abstract

The utility model discloses a kind of centrifuges for high-efficiency polypeptide extraction, it includes box, the left surface of the box is fixedly connected with drain pipe, the outer surface of the drain pipe is provided with stop valve, the lower surface of the box is fixedly connected with first motor, the output of the first motor is fixedly connected with rotating shaft, the outer surface of the rotating shaft is fixedly connected with centrifugal tank, the rear surface of the box is fixedly connected with guide rail. By setting cleaning scraping strip, solid-phase residues adhered on the inner wall of centrifugal tank can be removed, and cooperating with guide rail, second motor, U-shaped frame, box cover, first threaded hole, bolt and second threaded hole and other structures, cleaning scraping strip can be disassembled, thereby improving practicality, by setting slot, sliding rod, limit block, spring, plug, inclined block, trapezoidal block, pressure block and jack etc. Structure, filter screen can be replaced according to separation requirement, thereby improving use effect.

Description

Technical Field

[0001] This utility model relates to the field of centrifuge technology, and in particular to a high-efficiency centrifuge for polypeptide extraction. Background Technology

[0002] In the industrial production system of peptides, the centrifuge is the core equipment in the solid-liquid separation process. Through the centrifugal force generated by high-speed rotation, it achieves efficient separation of the "target peptide liquid phase" from the "solid residue (cell debris, protein precipitates, raw material fibers, etc.)" in the crude peptide extract, providing a highly clear raw material solution for subsequent chromatographic purification, freeze-drying, and other processes. This equipment is widely applicable to the extraction of peptides from animal and plant sources, as well as microbial peptides.

[0003] However, existing centrifuges for peptide extraction still have certain drawbacks. For example, the particle size of impurities varies significantly at different stages of peptide extraction (coarse separation, fine separation) and for different types of raw materials. Traditional centrifuge filters are mostly fixed structures integrated with the feed tank, and the pore size is not adjustable. This results in the need to equip multiple centrifuges with different filter pore sizes during peptide extraction, which is not practical. Utility Model Content

[0004] The purpose of this invention is to provide a high-efficiency centrifuge for peptide extraction, which allows for filter replacement according to separation requirements, thereby improving the performance.

[0005] To achieve the above objectives, a high-efficiency centrifuge for peptide extraction is provided, comprising:

[0006] The container has a drain pipe fixedly connected to its left surface, and a shut-off valve on the outer surface of the drain pipe. A first motor is fixedly connected to the lower surface of the container, and a rotating shaft is fixedly connected to the output end of the first motor. A centrifuge tank is fixedly connected to the outer surface of the rotating shaft. A guide rail is fixedly connected to the rear surface of the container, and a second motor is fixedly connected to the lower end of the guide rail. A threaded rod is fixedly connected to the output end of the second motor. A U-shaped frame is threadedly connected to the outer surface of the threaded rod. A container cover is fixedly connected to the end of the U-shaped frame away from the threaded rod. A first threaded hole is provided on the upper surface of the container cover, and a bolt is threadedly connected to the inner surface of the first threaded hole. A connecting plate is threadedly connected to the outer surface of the bolt. A sealing ring is provided on the lower surface of the connecting plate, and a cleaning scraper is fixedly connected to the lower surface of the connecting plate.

[0007] The centrifuge tank has an opening on its outer surface. A fixing frame is fixedly connected to the inner surface of the opening. A slot is provided at the upper end of the fixing frame. A filter screen is slidably connected to the inner surface of the slot. A cavity is provided inside the fixing frame. A sliding rod is fixedly connected to the inner surface of the cavity. A limit block is fixedly connected to the end of the sliding rod away from the fixing frame. A spring is provided on the outer surface of the sliding rod. An insert block is slidably connected to the outer surface of the sliding rod. An inclined block is slidably connected to the outer surface of the insert block. A trapezoidal block is slidably connected to the inner surface of the cavity. A pressure block is fixedly connected to the upper surface of the trapezoidal block. An insertion hole is provided on the outer surface of the filter screen.

[0008] According to the centrifuge for high-efficiency polypeptide extraction, the inner surface of the insertion hole is adapted to the insertion block.

[0009] According to the centrifuge for high-efficiency polypeptide extraction, the outer surface of the cover is provided with a through hole, and the inner surface of the through hole is slidably connected to a cleaning scraper.

[0010] According to the centrifuge for high-efficiency peptide extraction, the outer surface of the connecting plate is provided with a second threaded hole, the inner surface of the second threaded hole is threadedly connected to a bolt, and the second threaded hole corresponds to the first threaded hole.

[0011] According to the centrifuge for high-efficiency peptide extraction, the upper end of the fixed frame is provided with a limiting groove, and the inner surface of the limiting groove is slidably connected to the pressure block.

[0012] According to the high-efficiency polypeptide extraction centrifuge, the outer surface of the rotating shaft is rotatably connected to the housing, the inner surface of the centrifuge tank is in contact with the cleaning scraper, and the outer surface of the threaded rod is rotatably connected to the guide rail.

[0013] According to the centrifuge for high-efficiency polypeptide extraction, the outer surface of the limiting block is slidably connected to the insert block, and the outer surface of the inclined block is slidably connected to the trapezoidal block.

[0014] According to the centrifuge for high-efficiency polypeptide extraction, both the first motor and the second motor are electrically connected to an external power source.

[0015] The above-mentioned solution has the following beneficial effects:

[0016] 1. By setting up cleaning scrapers, solid residues adhering to the inner wall of the centrifuge can be removed. In addition, with the addition of guide rails, a second motor, a U-shaped frame, a cover, a first threaded hole, bolts and a second threaded hole, the cleaning scrapers can be disassembled, thereby improving practicality.

[0017] 2. By setting up structures such as slots, slides, limit blocks, springs, inserts, inclined blocks, trapezoidal blocks, pressure blocks, and insertion holes, the filter screen can be replaced according to separation requirements, thereby improving the performance.

[0018] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0019] The present invention will be further described below with reference to the accompanying drawings and embodiments;

[0020] Figure 1 This is a schematic diagram of the overall structure of a high-efficiency polypeptide extraction centrifuge according to the present invention;

[0021] Figure 2 This is a cross-sectional view of the overall structure of a high-efficiency polypeptide extraction centrifuge according to the present invention.

[0022] Figure 3 for Figure 2 Enlarged structural diagram at point A;

[0023] Figure 4 This is a longitudinal sectional view of the overall structure of a high-efficiency polypeptide extraction centrifuge according to the present invention;

[0024] Figure 5 This is a partial structural breakdown diagram of a high-efficiency polypeptide extraction centrifuge according to the present invention;

[0025] Figure 6 This is a cross-sectional view of the internal structure of a centrifuge for high-efficiency polypeptide extraction according to the present invention.

[0026] Legend:

[0027] The components are as follows: 1. Box body; 2. Drain pipe; 3. Shut-off valve; 4. First motor; 5. Rotating shaft; 6. Centrifuge tank; 7. Guide rail; 8. Second motor; 9. U-shaped frame; 10. Box cover; 11. First threaded hole; 12. Bolt; 13. Connecting plate; 14. Sealing ring; 15. Cleaning scraper; 16. Opening; 17. Fixing frame; 18. Slot; 19. Filter screen; 20. Cavity; 21. Slide rod; 22. Limiting block; 23. Spring; 24. Insertion block; 25. Inclined block; 26. Trapezoidal block; 27. Pressure block; 28. Insertion hole; 29. ​​Through hole; 30. Second threaded hole; 31. Limiting groove; 32. Threaded rod. Detailed Implementation

[0028] This section will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the accompanying drawings. The purpose of the drawings is to supplement the textual description with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present utility model, but they should not be construed as limiting the scope of protection of the present utility model.

[0029] Reference Figure 1-6 This utility model discloses a high-efficiency polypeptide extraction centrifuge, comprising: a housing 1, a drain pipe 2 fixedly connected to the left surface of the housing 1, a shut-off valve 3 provided on the outer surface of the drain pipe 2, a first motor 4 fixedly connected to the lower surface of the housing 1, a rotating shaft 5 fixedly connected to the output end of the first motor 4, a centrifuge tank 6 fixedly connected to the outer surface of the rotating shaft 5, a guide rail 7 fixedly connected to the rear surface of the housing 1, a second motor 8 fixedly connected to the lower end of the guide rail 7, a threaded rod 32 fixedly connected to the output end of the second motor 8, a U-shaped frame 9 threadedly connected to the outer surface of the threaded rod 32, a housing cover 10 fixedly connected to the end of the U-shaped frame 9 away from the threaded rod 32, a first threaded hole 11 provided on the upper surface of the housing cover 10, and a bolt 12 threadedly connected to the inner surface of the first threaded hole 11. A connecting plate 13 is threaded onto the outer surface of bolt 12. A sealing ring 14 is provided on the lower surface of connecting plate 13. A cleaning scraper 15 is fixedly connected to the lower surface of connecting plate 13. A through hole 29 is provided on the outer surface of the box cover 10. The inner surface of the through hole 29 is slidably connected to the cleaning scraper 15, which facilitates the installation and removal of the cleaning scraper 15. A second threaded hole 30 is provided on the outer surface of connecting plate 13. The inner surface of the second threaded hole 30 is threaded onto bolt 12. The second threaded hole 30 corresponds to the first threaded hole 11, which facilitates the installation of the cleaning scraper 15. The outer surface of rotating shaft 5 is rotatably connected to box 1. The inner surface of centrifuge tank 6 is in contact with the cleaning scraper 15. The outer surface of threaded rod 32 is rotatably connected to guide rail 7. The first motor 4 and the second motor 8 are both electrically connected to an external power source.

[0030] The centrifuge tank 6 has an opening 16 on its outer surface. A fixing frame 17 is fixedly connected to the inner surface of the opening 16. A slot 18 is provided at the upper end of the fixing frame 17. A filter screen 19 is slidably connected to the inner surface of the slot 18. A cavity 20 is provided inside the fixing frame 17. A slide rod 21 is fixedly connected to the inner surface of the cavity 20. A limit block 22 is fixedly connected to the end of the slide rod 21 away from the fixing frame 17. A spring 23 is provided on the outer surface of the slide rod 21. An insert block 24 is slidably connected to the outer surface of the slide rod 21. An inclined plate is slidably connected to the outer surface of the insert block 24. A trapezoidal block 26 is slidably connected to the inner surface of the cavity 20 of the surface block 25. A pressure block 27 is fixedly connected to the upper surface of the trapezoidal block 26. An insertion hole 28 is provided on the outer surface of the filter screen 19. The inner surface of the insertion hole 28 is adapted to the insertion block 24 to facilitate the fixation of the filter screen 19. A limiting groove 31 is provided at the upper end of the fixing frame 17. The inner surface of the limiting groove 31 is slidably connected to the pressure block 27 to limit the movement of the pressure block 27. The outer surface of the limiting block 22 is slidably connected to the insertion block 24. The outer surface of the inclined block 25 is slidably connected to the trapezoidal block 26.

[0031] Those skilled in the art should connect all electrical components in this case to their compatible external power supply via wires, and should select a suitable controller according to the actual situation to meet the control requirements. The specific connection and control sequence should refer to the working principle described below, where the electrical components are connected in sequence. The detailed connection methods are well-known in the art. The following mainly introduces the working principle and process, and will not describe the electrical control further.

[0032] Working principle: During operation, the second motor 8 is started, driving the threaded rod 32 to move the lid 10 upward under the action of the U-shaped frame 9, thereby exposing the opening at the top of the centrifuge tank 6. Then, peptide raw materials are poured into the centrifuge tank 6, and the second motor 8 is started in reverse, driving the lid 10 to move downward again to engage with the tank body 1. Subsequently, the first motor 4 is started, driving the rotating shaft 5 to rotate the centrifuge tank 6. At this time, the peptide raw materials are centrifuged inside the centrifuge tank 6, and under the action of centrifugal force, the target liquid in the peptide raw materials is centrifuged. After impurities are filtered through the filter screen 19 inside the fixed frame 17, the liquid phase containing peptides is thrown into the space between the housing 1 and the centrifuge tank 6. The target liquid phase is then discharged and extracted via the drain pipe 2 by opening the shut-off valve 3. During the rotation of the centrifuge tank 6, its inner wall continuously contacts the cleaning scraper 15, reducing the adhesion of solid residue inside the centrifuge tank 6. When the rotating shaft 5 needs replacement, simply loosen and remove the bolt 12, then pull out the connecting plate 13 and the cleaning scraper 15 along the through hole 29. Disassembly can be completed, and then the new cleaning scraper 15 is aligned with the through hole 29 and inserted. Then, the bolt 12 is screwed into the first threaded hole 11 and the second threaded hole 30 to complete the overall installation of the new cleaning scraper 15. When it is necessary to replace the filter screen 19 with a different aperture, simply push the pressure block 27 along the inside of the limiting groove 31 to drive the trapezoidal block 26 to slide along the inclined surface on the inclined block 25. This will cause the insert block 24 to retract into the cavity 20 along the slide rod 21 and compress the spring 23, thereby causing the insert block 24 to... After removing the filter 19 from the inside of the socket 28, and then opening the cover 10, simply pull the filter 19 out along the inside of the slot 18 to complete the disassembly. Then insert the new filter 19 with the required pore size into the slot 18. When the socket 28 on the new filter 19 corresponds to the insert block 24, release the pressure block 27. Under the rebound force of the spring 23, the insert block 24 will automatically insert into the socket 28, thereby completing the installation of the new filter 19 so as to remove impurities of different particle sizes in the target liquid phase as needed.

[0033] In the description of this utility model, it should be understood that the terms "coaxial," "bottom," "one end," "top," "middle," "other end," "upper," "side," "top," "inner," "front," "center," and "both ends," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. At the same time, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "fixed installation," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two elements or the interaction relationship between two elements. Unless otherwise explicitly limited, those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0034] The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.

Claims

1. A high-efficiency centrifuge for polypeptide extraction, characterized in that, include: A housing (1) is provided with a drain pipe (2) fixedly connected to its left surface. A shut-off valve (3) is provided on the outer surface of the drain pipe (2). A first motor (4) is fixedly connected to the lower surface of the housing (1). A rotating shaft (5) is fixedly connected to the output end of the first motor (4). A centrifuge tank (6) is fixedly connected to the outer surface of the rotating shaft (5). A guide rail (7) is fixedly connected to the rear surface of the housing (1). A second motor (8) is fixedly connected to the lower end of the guide rail (7). A threaded rod is fixedly connected to the output end of the second motor (8). 32), the outer surface of the threaded rod (32) is threaded with a U-shaped frame (9), and the end of the U-shaped frame (9) away from the threaded rod (32) is fixedly connected with a box cover (10). The upper surface of the box cover (10) is provided with a first threaded hole (11), the inner surface of the first threaded hole (11) is threaded with a bolt (12), the outer surface of the bolt (12) is threaded with a connecting plate (13), the lower surface of the connecting plate (13) is provided with a sealing ring (14), and the lower surface of the connecting plate (13) is fixedly connected with a cleaning scraper (15). The centrifuge tank (6) has an opening (16) on its outer surface. A fixing frame (17) is fixedly connected to the inner surface of the opening (16). A slot (18) is provided at the upper end of the fixing frame (17). A filter screen (19) is slidably connected to the inner surface of the slot (18). A cavity (20) is provided inside the fixing frame (17). A slide rod (21) is fixedly connected to the inner surface of the cavity (20). The slide rod (21) is located away from the fixing frame (17). One end is fixedly connected to a limiting block (22), the outer surface of the slide rod (21) is provided with a spring (23), the outer surface of the slide rod (21) is slidably connected to an insert block (24), the outer surface of the insert block (24) is slidably connected to an inclined block (25), the inner surface of the cavity (20) is slidably connected to a trapezoidal block (26), the upper surface of the trapezoidal block (26) is fixedly connected to a pressure block (27), and the outer surface of the filter screen (19) is provided with an insertion hole (28).

2. The high-efficiency polypeptide extraction centrifuge according to claim 1, characterized in that, The inner surface of the socket (28) is adapted to the plug (24).

3. The high-efficiency polypeptide extraction centrifuge according to claim 1, characterized in that, The outer surface of the box cover (10) is provided with a through hole (29), and the inner surface of the through hole (29) is slidably connected to the cleaning scraper (15).

4. A high-efficiency polypeptide extraction centrifuge according to claim 1, characterized in that, The outer surface of the connecting plate (13) is provided with a second threaded hole (30), the inner surface of the second threaded hole (30) is threadedly connected to the bolt (12), and the second threaded hole (30) corresponds to the first threaded hole (11).

5. A high-efficiency polypeptide extraction centrifuge according to claim 1, characterized in that, The upper end of the fixed frame (17) is provided with a limiting groove (31), and the inner surface of the limiting groove (31) is slidably connected to the pressure block (27).

6. A high-efficiency polypeptide extraction centrifuge according to claim 1, characterized in that, The outer surface of the rotating shaft (5) is rotatably connected to the box body (1), the inner surface of the centrifuge tank (6) is in contact with the cleaning scraper (15), and the outer surface of the threaded rod (32) is rotatably connected to the guide rail (7).

7. A high-efficiency polypeptide extraction centrifuge according to claim 1, characterized in that, The outer surface of the limiting block (22) is slidably connected to the insert block (24), and the outer surface of the inclined block (25) is slidably connected to the trapezoidal block (26).

8. A high-efficiency polypeptide extraction centrifuge according to claim 1, characterized in that, Both the first motor (4) and the second motor (8) are electrically connected to an external power source.

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