Recombinant adeno-associated viral vector purification device

Abstract

The utility model discloses a recombination adenovirus vector purification device, the utility model relates to purification device technical field, including the processing jar, the annular collection cavity is fixedly connected with the processing jar bottom, and the vertical direction between processing jar and annular collection cavity is installed with centrifugal mechanism, the utility model's advantage lies in: through the uniform distribution multiple stirring vane groups in annular collection cavity, when motor drive cylinder carries out centrifugal, and distributed stirring vane group rotates and makes the disinfectant and the cavity impurity liquid fully mixed, reduces the disinfection dead angle, and prevents the impurity aggregation or deposition, through the corresponding setting of the thrust in the upper hole, through the electric push rod drive makes it lift, can directly powerfully destroy the clogging impurity in the through -hole, and open the flow path, prevent the impurity liquid to gather not in annular collection cavity, and then keep the impurity liquid stable discharge, improve centrifugal efficiency.

Description

Technical Field

[0001] This utility model relates to the field of purification device technology, specifically a purification device for recombinant adeno-associated virus vectors. Background Technology

[0002] Adenoviruses are purified by centrifugation, which separates the supernatant and cell pellet. By removing impurities such as host cell proteins, unpackaged viral genomes, and culture medium residues, the risk of immune response is reduced, the effectiveness of experiments or treatments is improved, and potential side effects are avoided.

[0003] The applicant discovered through a search that a Chinese patent discloses "an adenovirus vector purification device" with publication number "CN221544557U". The impurity liquid centrifuged in this patent is discharged into the collection chamber through a through hole and mixed with disinfectant. Relying solely on natural diffusion and liquid flow, the mixing efficiency is low. In particular, viscous or particulate impurities lead to disinfection dead zones, and the through hole becomes clogged due to impurities passing through for a long time, thus affecting the flow of impurity liquid. Therefore, we propose a recombinant adeno-associated virus vector purification device. Utility Model Content

[0004] The purpose of this invention is to provide a device for purifying recombinant adeno-associated virus vectors.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a recombinant adeno-associated virus vector purification device, comprising a processing tank, an annular collection chamber fixedly connected to the bottom of the processing tank, and a centrifugation mechanism installed vertically between the processing tank and the annular collection chamber.

[0006] The annular collection cavity is evenly distributed with stirring blades in the circumferential direction. The stirring blades pass through the bottom of the annular collection cavity through bearings. A through hole is opened at the bottom edge of the treatment tank, and the through hole communicates with the annular collection cavity. Limiting grooves are arranged on the circumferential sidewalls below the treatment tank. Limiting components are slidably connected in the limiting grooves. Connecting rings are fixedly connected between the limiting components. The bottom of the connecting rings is provided with barbs.

[0007] As a further embodiment of this utility model: a tank cover is detachably installed on the top of the processing tank, and electric push rods are symmetrically provided at both ends of the tank cover. The output end of the electric push rod passes through the tank cover through a bearing and is connected to a connecting ring.

[0008] As a further embodiment of this utility model: the number of through holes is the same as the number of punctures, and each puncture corresponds to one above a through hole.

[0009] As a further embodiment of this utility model: the centrifugal mechanism includes a cylinder, and the cylinder is provided with locking grooves arranged in the circumferential direction. Each locking groove is provided with an ultrafiltration template by screws, and a positioning cylinder is fixedly connected to the middle side inside the cylinder.

[0010] As a further embodiment of this utility model: a support boss is fixedly connected to the middle of the bottom side inside the processing tank, and the upper end of the support boss protrudes and is embedded in the groove at the bottom of the cylinder.

[0011] As a further embodiment of this utility model: a motor is installed in the middle of the bottom side of the annular collection cavity, and a rotating rod is provided at the output end of the motor. The rotating rod passes through the top side of the annular collection cavity and the bottom of the treatment tank through a bearing. The rotating rod is sleeved in the positioning cylinder and is limited by a protrusion.

[0012] As a further embodiment of this utility model: a drive gear is provided on the outer wall below the rotating rod, and the teeth of the drive gear in the circumferential direction are all meshed with driven gears, and the output end of the driven gear is connected to the stirring blade assembly.

[0013] Compared with the prior art, the beneficial effects of this utility model by adopting the above technical solution are as follows:

[0014] 1. This utility model uses multiple stirring blade groups evenly distributed in the annular collection chamber. When the motor drives the cylinder to centrifuge, the distributed stirring blade groups rotate, thereby making the disinfectant liquid and the impurity liquid in the chamber fully mixed, reducing the disinfection dead zone, and preventing the accumulation or precipitation of impurities.

[0015] 2. This utility model, by setting a corresponding thorn above the through hole and driving it to rise and fall by an electric push rod, can directly and forcefully destroy the blockage impurities in the through hole, open the flow path, prevent the impurity liquid from accumulating in the annular collection chamber, and thus maintain the stable discharge of the impurity liquid.

[0016] Other advantages, objectives and features of this invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination or study, or may be taught from the practice of this invention. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the overall structure in an embodiment of this utility model;

[0018] Figure 2 This is a partial cross-sectional view of an embodiment of the present utility model;

[0019] Figure 3 This is a schematic diagram of the interior of the processing tank in an embodiment of this utility model;

[0020] Figure 4This is a schematic diagram of the disassembled structure of the centrifuge cylinder in an embodiment of this utility model;

[0021] Figure 5 This is a schematic diagram of the stabbing action in an embodiment of the present invention;

[0022] Figure 6 This is a schematic diagram of the connection between the rotating rod and the sleeve in an embodiment of this utility model.

[0023] In the diagram: 1. Processing tank; 11. Limiting groove; 2. Tank cover; 3. Support boss; 4. Centrifuge mechanism; 41. Cylinder; 42. Engaging groove; 43. Ultrafiltration template; 44. Rotating rod; 46. Motor; 47. Positioning cylinder; 5. Annular collection chamber; 51. Stirring blade assembly; 52. Driven gear; 53. Driven gear; 6. Through hole; 7. Electric push rod; 8. Connecting ring; 81. Limiting component; 9. Stinger. Detailed Implementation

[0024] The specific embodiments of this utility model will be further described below with reference to the accompanying drawings. It should be noted that the description of these embodiments is for the purpose of helping to understand this utility model, but does not constitute a limitation on this utility model.

[0025] Furthermore, the technical features involved in the various embodiments of this utility model described below can be combined with each other as long as they do not conflict with each other.

[0026] Please see the appendix Figure 1 - Appendix Figure 6 The present invention relates to a recombinant adeno-associated virus vector purification device, comprising a processing tank 1, an annular collection chamber 5 fixedly connected to the bottom of the processing tank 1, and a centrifugation mechanism 4 installed vertically between the processing tank 1 and the annular collection chamber 5.

[0027] In Example 1, stirring blades 51 are evenly distributed around the circumference of the annular collection cavity 5, and the stirring blades 51 pass through the bottom of the annular collection cavity 5 via bearings.

[0028] Specifically, a drive gear 53 is provided on the outer wall below the rotating rod 44. The teeth of the drive gear 53 in the circumferential direction are all meshed with the driven gear 52. The output end of the driven gear 52 is connected to the stirring blade assembly 51.

[0029] In this embodiment, multiple stirring blade groups 51 are evenly distributed in the annular collection chamber 5. When the motor 46 drives the cylinder 41 to centrifuge, the distributed stirring blade groups 51 rotate, thereby making the disinfectant and the impurity liquid in the chamber fully mixed. A sealing element is provided at the connection between the stirring blade group 51 and the annular collection chamber 5 to prevent leakage.

[0030] In embodiment 2, a through hole 6 is provided at the bottom edge of the treatment tank 1, which is connected to the annular collection cavity 5. Limiting grooves 11 are arranged on the side wall of the treatment tank 1 in the circumferential direction below. Limiting components 81 are slidably connected in the limiting grooves 11. Connecting rings 8 are fixedly connected between the limiting components 81. The bottom of the connecting rings 8 is provided with barbs 9.

[0031] Specifically, a can lid 2 is detachably installed on the top of the processing tank 1. Electric push rods 7 are symmetrically provided at both ends of the can lid 2. The output end of the electric push rod 7 passes through the can lid 2 through a bearing and is connected to the connecting ring 8.

[0032] In this embodiment, the electric push rod 7 causes the piercing 9 to extend and retract within the through hole 6, thus opening up the flow path.

[0033] Specifically, the number of through holes 6 is the same as the number of punctures 9, and each puncture 9 corresponds to one above the through hole 6.

[0034] In this embodiment, the one-to-one correspondence design allows the impurities accumulated in the through hole 6 to be cleared, and a notch is opened at the edge of the connecting ring 8 so that the impurity liquid discharged from the centrifugation can be discharged from the through hole 6 through the notch, avoiding obstruction.

[0035] In embodiment 3, the centrifugal mechanism 4 includes a cylinder 41, and the cylinder 41 has locking grooves 42 arranged in the circumferential direction. Each locking groove 42 is provided with an ultrafiltration template 43 by screws. A positioning cylinder 47 is fixedly connected to the middle side inside the cylinder 41.

[0036] Specifically, a support boss 3 is fixedly connected to the middle of the bottom side inside the treatment tank 1, and the upper end of the support boss 3 protrudes and is embedded in the bottom groove of the cylinder 41.

[0037] In this embodiment, the detachable ultrafiltration template 43 facilitates the removal of trapped pancreatic virus vectors and makes it easy to replace the ultrafiltration template 43. In addition, the support boss 3 can abut against the bottom groove of the cylinder 41, further improving the stability of the centrifugation process.

[0038] Specifically, a motor 46 is installed in the middle of the bottom side of the annular collection chamber 5. A rotating rod 44 is provided at the output end of the motor 46. The rotating rod 44 passes through the top side of the annular collection chamber 5 and the bottom of the treatment tank 1 through a bearing. The rotating rod 44 is sleeved in the positioning cylinder 47 and is limited by a protrusion.

[0039] In this embodiment, the centrifuge tube is fitted onto the rotating rod 44 by a positioning tube 47, and is limited by the protrusions and grooves around it, thereby improving the stability of the centrifuge tube during the centrifugation process.

[0040] Working principle: The liquid to be separated and purified is poured into the cylinder 41, and then the lid 2 is closed. The motor 46 is started to drive the rotating rod 44 to rotate. The protrusion on the rotating rod 44 drives the cylinder 41 to rotate, which can centrifuge the pancreatic virus vector. During the centrifugation process, the impurity liquid is discharged into the processing tank 1 through the ultrafiltration template 43. Since there is a notch on the connecting ring 8, the impurity liquid enters the annular collection chamber 5 through the through hole 6. Some of the impurities remaining on the connecting ring 8 can be manually cleaned off when the cylinder 41 is disassembled later. At the same time, impurities that do not pass through the through hole 6 can be manually scraped into the annular collection chamber 5. Inside; driven by the motor 46, the active gear 53 rotates, causing the driven gears 52 around it to rotate, which in turn drives the stirring blade assembly 51 to rotate. The rotation of the distributed stirring blade assembly 51 allows the disinfectant to be fully mixed with the impurities in the chamber. During centrifugation, the electric push rod 7 can be activated at regular intervals to move the stab 9 toward the stab 6, breaking up the impurities blocking the through hole 6 and opening the flow path. After centrifugation, the user can disassemble and open the can lid 2, and pull it up to separate the can lid 2 from the cylinder 41. The centrifuge cylinder can then be pulled upwards to remove it, allowing the pancreatic virus vector to be extracted.

[0041] The terms "front," "back," "left," "right," "top," and "bottom" all refer to the figures in the accompanying drawings. Figure 1 Based on.

[0042] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of this utility model.

[0043] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments.

[0044] For those skilled in the art, various changes, modifications, substitutions, and alterations to these embodiments without departing from the principles and spirit of this utility model will still fall within the protection scope of this utility model.

Claims

1. A recombinant adeno-associated virus vector purification apparatus, comprising a processing tank (1), characterized in that: The bottom of the treatment tank (1) is fixedly connected to an annular collection chamber (5), and a centrifugal mechanism (4) is installed in the vertical direction between the treatment tank (1) and the annular collection chamber (5); The annular collection cavity (5) has a uniformly distributed stirring blade assembly (51) in the circumferential direction. The stirring blade assembly (51) passes through the bottom of the annular collection cavity (5) through a bearing. A through hole (6) is provided at the bottom edge of the treatment tank (1). The through hole (6) communicates with the annular collection cavity (5). Limiting grooves (11) are arranged on the side wall in the circumferential direction below the treatment tank (1). Limiting components (81) are slidably connected in the limiting grooves (11). Connecting rings (8) are fixedly connected between the limiting components (81). The bottom of the connecting rings (8) is provided with barbs (9).

2. The recombinant adeno-associated virus vector purification device according to claim 1, characterized in that: The top of the processing tank (1) is detachably fitted with a tank cover (2). The two ends of the tank cover (2) are symmetrically provided with electric push rods (7). The output end of the electric push rod (7) passes through the tank cover (2) through a bearing and is connected to the connecting ring (8).

3. The recombinant adeno-associated virus vector purification device according to claim 1, characterized in that: The number of through holes (6) is the same as the number of thorns (9), and each thorn (9) corresponds to one above the through hole (6).

4. The recombinant adeno-associated virus vector purification device according to claim 1, characterized in that: The centrifugal mechanism (4) includes a cylinder (41), and the cylinder (41) has locking grooves (42) arranged in the circumferential direction. Each locking groove (42) is provided with an ultrafiltration template (43) by screws. A positioning cylinder (47) is fixedly connected to the middle side inside the cylinder (41).

5. The recombinant adeno-associated virus vector purification device according to claim 1, characterized in that: A support boss (3) is fixedly connected to the middle of the bottom side inside the processing tank (1), and the upper end of the support boss (3) protrudes and is embedded in the bottom groove of the cylinder (41).

6. The recombinant adeno-associated virus vector purification apparatus according to claim 1, characterized in that: A motor (46) is installed in the middle of the bottom side of the annular collection cavity (5). A rotating rod (44) is provided at the output end of the motor (46). The rotating rod (44) passes through the top side of the annular collection cavity (5) and the bottom of the treatment tank (1) through a bearing. The rotating rod (44) is sleeved in the positioning cylinder (47) and is limited by a protrusion.

7. The recombinant adeno-associated virus vector purification apparatus according to claim 6, characterized in that: The lower outer wall of the rotating rod (44) is provided with a drive gear (53), and the teeth of the drive gear (53) in the circumferential direction are all meshed with driven gears (52). The output end of the driven gear (52) is connected to the stirring blade assembly (51).

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