Intelligent multi-gas substrate fermentation equipment

Abstract

The utility model belongs to the technical field of biological fermentation equipment relates to an intelligent multi -gas substrate fermentation equipment, has solved the technical problem that the poor adaptability and mixed performance of existing fermentation equipment stirring structure. At least one group of composite leaf subassembly is provided on the stirring shaft, each group of composite leaf subassembly includes a plurality of composite leaves, each composite leaf includes first blade, second blade, first blade is vertically provided, and the second blade is obliquely arranged at the top end of the first blade, the stirring shaft is uniformly spaced and is provided with a plurality of outer threads along its axial direction, the fixed disc is provided with the through -hole that adapts to the stirring shaft, the fixed disc is provided with the internal thread that adapts to the outer thread on the inner circumferential side wall, and the fixed disc is fixedly connected with the stirring shaft through the thread connection mode, thereby the position of the inclined leaf subassembly, the composite leaf subassembly on the stirring shaft is adjustable, and the different liquid level height in the fermentation tank body is adapted.

Description

Technical Field

[0001] This utility model belongs to the technical field of biological fermentation equipment, specifically relating to an intelligent multi-gas substrate fermentation device. Background Technology

[0002] During the in vitro fermentation culture of various biological cells or organic matter, the fermentation process has stringent requirements for the reaction environment. It requires precise matching of different gas ratios, a stable sealed environment inside the tank, uniform substrate mixing, and dynamic control of multi-dimensional parameters. The comprehensive performance of the equipment directly determines the fermentation reaction efficiency, product purity, and production stability.

[0003] Currently, conventional multi-gas fermentation equipment on the market still has some technical shortcomings in practical applications, making it difficult to meet the demands of high-precision, intelligent, and highly stable industrial fermentation production. For example, conventional agitators are mostly fixed installation structures, which cannot flexibly adjust the installation position and working level according to different liquid levels in the fermentation broth, making it difficult to adapt to different fermentation batches and different substrate filling volumes. At the same time, traditional agitators have a simple structure, mostly only having a single horizontal stirring function, with weak axial material convection capacity. Dead zones are prone to substrate accumulation and insufficient gas-liquid mixing at the bottom of the fermentation tank, resulting in low mass transfer efficiency between gas and substrate and fermentation broth, uneven substrate concentration and gas distribution in the tank, significantly reducing the overall fermentation reaction rate and raw material utilization. The stirring systems of existing fermentation equipment mostly adopt traditional mechanical seal stirring structures, with the stirring shaft running through the tank. Long-term operation of mechanical seal structures is prone to wear and aging, leading to decreased tank sealing performance, insufficient pressure holding time, and easily causing problems such as contamination and leakage of reaction gases inside the tank.

[0004] Therefore, the existing fermentation equipment's stirring system still suffers from poor sealing performance, poor adaptability of the stirring structure, and poor mixing performance. Utility Model Content

[0005] The purpose of this invention is to provide an intelligent multi-gas substrate fermentation device, which features good substrate mixing performance, high sealing performance, and support for multi-gas ratios and multi-parameter intelligent control.

[0006] To achieve the above-mentioned objectives of this utility model, the technical solution adopted by this utility model is: an intelligent multi-gas substrate fermentation device, including a fermentation tank and a magnetic stirring system. The magnetic stirring system includes a stirring shaft driven by a magnetic component. An inclined blade assembly is provided at the lower end of the stirring shaft. At least one set of composite blade assemblies is provided on the stirring shaft and above the inclined blade assembly. Each set of composite blade assemblies includes several composite blades. Each composite blade includes a first blade and a second blade. The first blade is vertically arranged, and the second blade is inclinedly arranged at the top of the first blade. Multiple fixed discs are spaced apart on the stirring shaft. An inclined blade assembly or a composite blade assembly is fixedly mounted on the outer circumferential sidewall of each fixed disc. Multiple external threads are evenly spaced along the axial direction of the stirring shaft. The fixed discs have through holes adapted to the stirring shaft. The inner circumferential sidewalls of the fixed discs have internal threads adapted to the external threads. The fixed discs are fixedly connected to the stirring shaft by a threaded connection, thereby making the positions of the inclined blade assembly and the composite blade assembly on the stirring shaft adjustable to adapt to different liquid levels in the fermenter.

[0007] Preferably, each of the fixed disks is provided with limit blocks at its upper and lower ends and around the stirring shaft. The limit blocks are threadedly connected to the stirring shaft. By providing limit blocks on the upper and lower sides of the fixed disk, the position of the fixed disk on the stirring shaft is restricted.

[0008] Preferably, the magnetic component includes an inner magnetic ring and an outer magnetic ring. A servo motor is provided on the top of the fermenter body. The outer magnetic ring is fixedly installed at the output end of the servo motor. The inner magnetic ring is fixedly installed on the top of the stirring shaft. The inner and outer magnetic rings are magnetically coupled.

[0009] Preferably, the oblique blade assembly includes a plurality of oblique blades, wherein the oblique blades are suction-type blades.

[0010] Preferably, a defoaming slurry assembly is also connected to the stirring shaft via a fixed plate. The defoaming slurry assembly is located above the uppermost composite blade assembly. The defoaming slurry assembly includes several defoaming slurries, each of which includes a defoaming part body. Several defoaming protrusions are provided on the circumferential sidewall of the defoaming part body. The shape of the defoaming protrusions includes frustum, truncated pyramid, cone, and pyramidal.

[0011] Preferably, an annular gas distributor is provided at the bottom of the fermenter body, and the annular gas distributor is connected to the ventilation system; the annular gas distributor includes a spiral disc tube connected to the air inlet pipe, and the spiral disc tube is provided with a plurality of air outlet holes, the air outlet holes being vented upwards.

[0012] Preferably, the fermentation tank includes a main tank and a temperature control jacket located outside the main tank. The temperature control jacket is filled with a heat exchange medium, which is derived from a water bath electric heater and a chiller.

[0013] Preferably, the fermentation equipment also includes a gas detection system, which includes a detection pipeline. The starting end and the ending end of the detection pipeline are both connected to the fermentation tank. A dryer and a tail gas analyzer are sequentially arranged on the detection pipeline in the direction of medium flow.

[0014] Preferably, the ventilation system includes an inlet pipe and an outlet pipe. The inlet pipe is connected to an H2 cylinder, an O2 cylinder, and a CO2 cylinder at its starting end, and to the fermentation tank at its ending end. Along the fluid flow direction, a pressure reducing valve, a mass flow meter, a pre-filter, and a fine filter are sequentially installed on the inlet pipe. The outlet pipe is connected to the fermentation tank at its starting end, and is equipped with a reflux condenser. The liquid outlet end of the reflux condenser is connected to the fermentation tank, and the gas outlet end is connected to the outside.

[0015] Preferably, the fermentation tank is equipped with a pressure transmitter, a temperature electrode, a defoaming electrode, a pH electrode, and a DO electrode; the fermentation equipment also includes an intelligent control system, which is communicatively connected to the pressure transmitter, temperature electrode, defoaming electrode, pH electrode, and DO electrode. The intelligent control system controls the opening degree of the air inlet valve and / or exhaust valve in real time based on the pressure inside the fermenter monitored by the pressure transmitter. The intelligent control system controls the temperature of the water bath electric heating or cold water cooling in real time based on the temperature inside the fermentation tank monitored by the temperature electrode. The intelligent control system controls the start and stop of the defoaming peristaltic pump in real time based on the fermentation foam monitored by the defoaming electrode, and replenishes the fermentation tank with chemical defoaming agent. The intelligent control system controls the start and stop of the acid replenishment peristaltic pump or the acid replenishment and depletion peristaltic pump in real time based on the pH value monitored by the pH electrode in the fermenter. The intelligent control system controls the servo motor power and the start / stop of the feed pump in real time based on the dissolved oxygen concentration in the fermenter monitored by the DO electrode.

[0016] This utility model has the following beneficial effects: 1. The stirring shaft of this fermentation equipment is evenly spaced with multiple external threads. The inner circumferential sidewall of the fixing plate that fixes the defoaming slurry assembly, composite blade assembly, and inclined blade assembly is provided with internal threads. The installation of the defoaming slurry assembly, composite blade assembly, and inclined blade assembly to the stirring shaft is achieved through threaded connection. This method of installation and disassembly is simple and efficient. It can adjust the position of the defoaming slurry assembly, composite blade assembly, and inclined blade assembly on the stirring shaft. It can also reasonably arrange the distance between the defoaming slurry assembly, composite blade assembly, and inclined blade assembly without the aid of measuring devices. At the same time, the number of composite blade assemblies can be appropriately increased or decreased according to actual needs to adapt to different liquid levels in the fermentation tank.

[0017] 2. This fermentation equipment features suction-type inclined blades below the stirring shaft. When these blades rotate, they create a negative pressure at the bottom of the fermentation tank, lifting the substrate upwards and improving mixing. Furthermore, a composite blade assembly above the inclined blades, consisting of a first blade and a second blade (the first blade is vertically positioned, and the second blade is angled at the top), increases the axial displacement of the composite blades due to their interaction. This further lifts the substrate upwards, ensuring thorough axial mixing and thus improving the overall mixing effect. This solves the problem of poor axial mixing capacity in existing stirring systems.

[0018] 3. The stirring system of this fermentation equipment adopts a magnetic stirring system. The magnetic field of the outer magnetic ring penetrates the fermentation tank and drives the inner magnetic ring, stirring shaft, defoaming slurry, composite blades and inclined blades in the fermentation tank to rotate synchronously without contact. Compared with the traditional stirring system, this stirring system has no mechanical seal and no leakage channel, has ideal dynamic balance performance and extremely high airtightness, and the pressure holding time can exceed 24 hours, eliminating the hidden dangers of contamination and gas leakage. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the system structure of the fermentation equipment of this utility model (the reflux condenser, etc. are not shown in the figure). Figure 2 This is a schematic diagram of the structure of the fermentation tank of this utility model; Figure 3 This is a three-dimensional structural diagram of the composite leaf of this utility model.

[0020] The following labels are used in the attached diagram: 1-Fermentation tank, 2-Tail gas analyzer, 3-Dryer, 4-Fine filter, 5-Pre-filter, 6-Mass flow meter, 7-Servo motor, 8-Outer magnetic ring, 9-Inner magnetic ring, 10-Mounting base, 11-Defoaming protrusion, 12-Defoaming slurry, 13-Agitator shaft, 14-External thread, 15-Composite blade, 16-Fixed disc, 17-Annular gas distributor, 18-Inclined blade, 19-Second blade, 20-First blade, 21-Limiting block. Detailed Implementation

[0021] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Unless otherwise specified, the technical means used in the embodiments are conventional means well known to those skilled in the art.

[0022] In the description of this utility model, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0023] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," "link," and "fix" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0024] like Figure 1-2 As shown, this utility model discloses an intelligent multi-gas substrate fermentation device, including a fermentation tank 1, a magnetic stirring system, an aeration system, a gas detection system, and an intelligent control system. An annular gas distributor 17 is provided at the bottom of the fermentation tank 1. The annular gas distributor 17 is connected to the aeration system. The aeration system mixes, filters, and sterilizes H2, O2, and CO2, and then inputs them into the annular gas distributor 17 through the air inlet pipe. The annular gas distributor 17 evenly delivers the mixed gas into the fermentation tank 1. The magnetic stirring system includes a magnetic component, a stirring shaft 13, a defoaming slurry component, a composite blade component, and an inclined blade component. The magnetic component includes an inner magnetic ring 9 and an outer magnetic ring 8. The defoaming slurry component includes several defoaming slurries 12. The composite blade component includes several composite blades 15. The inclined blade component includes several inclined blades 18. A servo motor 7 is fixedly installed on the top of the fermentation tank 1, and the outer magnetic ring 8 is fixedly installed at the output end of the servo motor 7. The inner magnetic ring 9 is fixedly installed on the top of the stirring shaft 13, and a mounting base 10 is fixedly installed on the top of the fermentation tank 1. The inner magnetic ring 9 and the top of the stirring shaft 13 are rotatably mounted on the mounting base 10. Both the fermentation tank 1 and the mounting base 10 are made of non-magnetic metal material. This utility model adopts... Made of 316L stainless steel, the magnetic field of the outer magnetic ring 8 penetrates the fermentation tank 1, driving the inner magnetic ring 9, stirring shaft 13, defoaming slurry 12, composite blades 15, and inclined blades 18 within the fermentation tank 1 to rotate synchronously without contact. This magnetic stirring system has no mechanical seal and no leakage channels. The inner magnetic ring 9 and outer magnetic ring 8 achieve contactless transmission through magnetic coupling. After the servo motor 7 starts, it drives the outer magnetic ring 8 to rotate, which in turn drives the stirring shaft 13 to rotate within the fermentation tank 1, replacing the traditional mechanical seal and ensuring a sterile environment. Along the axial direction of the stirring shaft 13, the positions of the defoaming slurry assembly, composite blade assembly, and inclined blade assembly on the stirring shaft 13 are adjustable to adapt to different liquid levels within the fermentation tank 1, thus accommodating different liquid level processes.

[0025] The magnetic stirring system used in the stirring part of this utility model has magnetic coupling between the outer magnetic ring 8 and the inner magnetic ring 9, which replaces the traditional mechanical seal. It has ideal dynamic balance performance and extremely high sealing performance, and the pressure holding time can exceed 24 hours, eliminating the hidden dangers of bacterial contamination and gas leakage.

[0026] In a specific embodiment, the fermenter body 1 of this utility model adopts a flat-top lid that is bolted on. The flat-top lid is provided with an exhaust port, a flame inoculation port that also serves as a feeding port and a cleaning port, an acid replenishment port, an alkali replenishment port, a material replenishment port, and a defoaming electrode port. The side wall of the fermenter body 1 is provided with an air inlet, a sterile sampling port, a temperature electrode port, a pH electrode port, and a DO electrode port.

[0027] In a further embodiment, the position of the defoaming slurry assembly, the composite blade assembly, and the inclined blade assembly on the stirring shaft 13 is adjustable as follows: a fixed disk 16 is provided on the stirring shaft 13, the fixed disk 16 has through holes adapted to the stirring shaft 13, the defoaming slurry assembly, the composite blade assembly, or the inclined blade assembly is arranged in a circumferential array on the outer circumferential sidewall of the fixed disk 16, multiple external threads 14 are evenly spaced along its axial direction on the stirring shaft 13, and internal threads adapted to the external threads 14 are provided on the inner circumferential sidewall of the fixed disk 16. The fixed disk 16 is fixedly connected to the stirring shaft 13 by a threaded connection. Limiting blocks 21 are provided at the upper and lower ends of each fixed disk 16 and on the periphery of the stirring shaft 13, and the limiting blocks 21 are threadedly connected to the stirring shaft 13. By providing limiting blocks 21 on the upper and lower sides of the fixed disk 16, the position of the fixed disk 16 on the stirring shaft 13 can be restricted. Furthermore, a locking screw is provided laterally on the outer wall of the limiting block 21. The locking screw is used to lock and fix the limiting block 21 to the stirring shaft 13. Correspondingly, the stirring shaft 13 is provided with a threaded hole corresponding to the locking screw to further fix and lock the limiting block 21.

[0028] The positions of the defoaming slurry assembly, composite blade assembly, and inclined blade assembly can be adjusted according to actual needs. The number of composite blade assemblies can also be adjusted as needed. Figure 2 As shown, two sets of composite blade components are set between the defoaming slurry component and the inclined blade component.

[0029] For ease of processing, the outer circumferential sidewall of the stirring shaft 13 can be entirely provided with external threads 14. In this case, the positions of the defoaming slurry 12, the composite blade 15, and the inclined blade 18 on the stirring shaft 13 can be continuously adjusted.

[0030] In a further embodiment, the oblique blade 18 is disposed below the composite blade 15. The oblique blade 18 is an inhalation blade, which is similar to a fan blade structure. The inhalation direction of the inhalation blade is upward. When the oblique blade 18 rotates, it creates a certain negative pressure at the bottom of the fermentation tank 1, which lifts the substrate at the bottom of the fermentation tank upward, thereby improving the mixing effect.

[0031] like Figure 3 As shown, the composite leaf 15 includes a first blade 20 and a second blade 19. The first blade 20 is vertically arranged, and the second blade 19 is inclinedly arranged at the top of the first blade 20. The first blade 20 and the second blade 19 are of the same length, and the multiple composite leaves 15 located on the same fixed plate 16 have the same orientation. Due to the interaction between the first blade 20 and the second blade 19, the axial displacement of the composite leaf 15 is increased, further lifting the substrate at the bottom of the fermenter upward, so that the substrate is fully mixed in the axial direction, and further improving the mixing effect.

[0032] To further improve the mixing effect, baffles can also be installed on the inner wall of fermentation tank 1.

[0033] In a further embodiment, the defoaming slurry 12 is located above the composite blade 15 and the inclined blade 18. The defoaming slurry 12 includes a defoaming part body, and a plurality of defoaming protrusions 11 are provided on the circumferential sidewall of the defoaming part body. The shapes of the defoaming protrusions 11 include frustum, truncated pyramid, conical, and pyramidal. The defoaming part body can be in the form of a defoaming shaft, defoaming blades, etc. By setting the defoaming slurry 12, foam in the fermentation tank 1 can be initially eliminated.

[0034] In a further embodiment, the annular gas distributor 17 includes a spiral disc tube connected to the air inlet pipe, and the spiral disc tube is provided with a plurality of air outlet holes, the air outlet holes being oriented upwards.

[0035] In a further embodiment, the fermentation tank 1 includes a main tank and a temperature control jacket located outside the main tank. The temperature control jacket is filled with a heat exchange medium derived from a water bath electric heater and a chiller. The water bath electric heater and chiller are existing technologies and are not part of the core improvement of this utility model; therefore, their specific structures will not be described in detail here.

[0036] In a further embodiment, the gas detection system includes a detection pipeline, the beginning and end of which are connected to the fermentation tank 1. A dryer 3 and a tail gas analyzer 2 are sequentially arranged on the detection pipeline according to the direction of medium flow. The dryer 3 and tail gas analyzer 2 utilize existing technology and are not core improvements of this invention; therefore, their specific structures will not be described in detail here.

[0037] In a further embodiment, the ventilation system includes an intake pipe and an exhaust pipe. The starting end of the intake pipe is connected to an H2 cylinder, an O2 cylinder, and a CO2 cylinder, and the end of the intake pipe is connected to the fermentation tank 1. Along the fluid flow direction, a pressure reducing valve, a mass flow meter 6, a pre-filter 5, and a fine filter 4 are sequentially installed on the intake pipe.

[0038] Because the high-temperature moisture and volatile organic compounds inside the fermentation tank 1 are easily lost with the exhaust gas during the ventilation and exhaust circulation, thus affecting the material balance, this utility model provides a reflux condenser on the exhaust pipe. The starting end of the exhaust pipe is connected to the fermentation tank 1, the liquid discharge end of the reflux condenser is connected to the fermentation tank 1, and the gas discharge end of the reflux condenser is connected to the outside.

[0039] This utility model's ventilation system automatically controls the multi-channel intake ratio of H2, O2, and CO2 using a digital mass flow meter 6. Combined with a gas detection system equipped with a drying function, it achieves precise gas delivery and real-time monitoring of exhaust gas component concentrations. Simultaneously, by installing a reflux condenser on the exhaust pipe, it ensures that organic matter and moisture generated during fermentation are not lost with the gas emissions.

[0040] In a further embodiment, the fermentation tank 1 is equipped with a pressure transmitter, a temperature electrode, a defoaming electrode, a pH electrode, and a DO electrode. The intelligent control system includes a programmable logic controller (PLC) and a touch screen, and is communicatively connected to the pressure transmitter, temperature electrode, defoaming electrode, pH electrode, DO electrode, gas detection system, acid replenishment peristaltic pump, alkali replenishment peristaltic pump, feed replenishment peristaltic pump, and defoaming peristaltic pump. The intelligent control system uses intelligent PID control to achieve linkage control between dissolved oxygen (DO) and the speed of the servo motor 7 or the feed replenishment peristaltic pump; and the increase or decrease of the motor speed is forcibly set with a buffer time of at least 30 seconds. The specific control methods of the intelligent control system are as follows: The intelligent control system controls the opening degree of the air inlet valve and / or exhaust valve in real time based on the pressure inside the fermentation tank 1 monitored by the pressure transmitter. The intelligent control system controls the temperature of the water bath electric heating or cold water cooling in real time based on the temperature inside the fermentation tank 1 monitored by the temperature electrode. The intelligent control system controls the start and stop of the defoaming peristaltic pump in real time based on the fermentation foam monitored by the defoaming electrode, and replenishes the fermentation tank 1 with chemical defoaming agent. The intelligent control system controls the start and stop of the acid replenishment peristaltic pump or the acid replenishment and depletion peristaltic pump in real time based on the pH value monitored by the pH electrode in the fermentation tank 1. The intelligent control system controls the power of the servo motor 7 and the start / stop of the feed pump in real time based on the dissolved oxygen concentration in the fermentation tank 1 monitored by the DO electrode.

[0041] Example 1

[0042] This embodiment relates to a stainless steel gas fermentation system with a full capacity of 10L, such as... Figure 1 As shown in the figure (not all equipment and structures are shown, only key equipment is shown), its liquid filling coefficient is 70%, and the working volume is between 3 and 8 L. The entire system mainly includes fermentation tank 1, external air compressor (40 L / min), refrigerated dryer (1 m³ / min), fully automatic water replenishment steam generator (6 kW), automatic water replenishment chiller (1 P), and MCBIO-6000 control system.

[0043] The fermenter has a diameter-to-height ratio of 1:2. The main tank body is made of SUS316 stainless steel, formed by automatic laser welding. The inner surface of the tank undergoes a dual process of mechanical polishing and electrolytic polishing, resulting in a surface roughness Ra≤0.4μm. The external temperature control jacket, with a 75% coverage area, is made of SUS304 stainless steel, featuring an optimized flow guiding structure and a mirror-polished exterior. A long, narrow observation mirror resistant to high temperatures (≥130℃) and high pressures (≥0.3MPa) is located at the front of the main tank, and a high-brightness LED lighting source is installed on the top. The main tank is designed to withstand pressures of -0.1 to 0.3MPa, while the jacket is designed to withstand pressures of 0.4MPa.

[0044] The main tank's bottom discharge port is equipped with a sanitary, dead-angle-free diaphragm bottom valve that completely empties and sterilizes the internal bacterial solution. The side sterile sampling port features a sterile flange and a sanitary independent sampling valve for constant-temperature sterilization. The tank top uses a bolt-on flat-top lid with vents, a flame inoculation port, an acid replenishment port, an alkali replenishment port, a material replenishment port, and a defoaming electrode port. All piping in contact with the material uses seamless, internally and externally polished 316L stainless steel tubing, and all valves, instruments, and pipes are installed using a closed-type sanitary clamp quick-connect method for easy disassembly, assembly, and sterilization.

[0045] The mixing section employs a magnetic stirring system without mechanical seals, driven by a 0.75kW Delta servo motor (Taiwan) 7 via an original driver. The 316L stainless steel mixing shaft 13 features a height-adjustable composite blade 15 and a two-stage mixing impeller (oblique blade 18), a layer of stainless steel mechanical pressure defoaming impeller, and four baffles for deep mixing. The speed setting range is 50–1000 rpm, and the software mandates a minimum 30-second buffer time for acceleration and deceleration.

[0046] The ventilation system supports a maximum ventilation flow rate of 2 VVM. External H2, O2, and CO2 gases are first depressurized (0–0.1 MPa) by a pressure reducing valve, and then precisely proportioned (accuracy ±1%) by a Swiss Vogtlin digital mass flow meter 6 with a range of 0–20 L / min. The gas then flows through a 0.2 μm pre-filter 5 and a 0.01 μm accurate German Sartorius hydrophobic PTFE fine filter 4 for sterilization, before being evenly blown in by an annular gas distributor 17 located at the bottom of the tank. A material check valve is also installed, and the exhaust end is connected to a 316L stainless steel reflux condenser and a breather filter.

[0047] The gas detection system uses Dynament online sensors from the UK (with a lifespan of over 5 years). After drying and gravity dehydration pretreatment by the front-end dryer 3, it measures the concentrations of H2 (0-5000ppm), O2 (0-25%vol), and CO2 (0-100%vol) in the exhaust gas in real time, with a measurement accuracy of <2%FS and a response time of ≤15 seconds.

[0048] The intelligent control system consists of a Siemens S7-200 series PLC and a 10-inch Kunlun Tongtai touch screen, and features real-time / historical curve recording and USB flash drive / Excel export functions. (1) Temperature control: German PT100 temperature electrode monitors in real time (0~150℃), and intelligent control system intelligent PID automatically coordinates water bath electric heating and cold water cooling to achieve precise temperature control in the range of 5~55℃ with an accuracy of ±0.1℃.

[0049] (2) pH control: The domestic high-end electrode (0-14) is resistant to high temperature of 121℃. The intelligent control system is linked to the acid / alkali replenishment peristaltic pump with a flow rate of 0-28mL / min, and the control accuracy reaches ±0.02pH.

[0050] (3) DO and feeding control: The DO range is between 0 and 200%. The intelligent control system can intelligently link the DO parameters with the servo motor speed of 7 or the feeding pump of 0 to 180 mL / min, and supports at least 10 segments of program flow feeding.

[0051] (4) Pressure control: The tank pressure is monitored by a Swiss Huba pressure transmitter, and the intelligent control system links the inlet and outlet valves to control the fermentation pressure between 0 and 0.3 MPa.

[0052] (5) Defoaming control: Fermentation foam is detected by a high-sensitivity defoaming electrode, and the intelligent control system automatically links the defoaming peristaltic pump to replenish the chemical defoaming agent and assists the mechanical paddle to complete the defoaming.

[0053] The embodiments described above are merely preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model. Any modifications, alterations, alterations, or substitutions made by those skilled in the art to the technical solutions of the present utility model without departing from the spirit of the present utility model shall fall within the protection scope defined by the claims of the present utility model.

Claims

1. An intelligent multi-gas substrate fermentation device, characterized in that: The system includes a fermentation tank (1) and a magnetic stirring system. The magnetic stirring system includes a stirring shaft (13) driven by a magnetic component. An inclined blade assembly is provided at the lower end of the stirring shaft (13). At least one set of composite blade assemblies is provided on the stirring shaft (13) and above the inclined blade assembly. Each set of composite blade assemblies includes several composite blades (15). Each composite blade (15) includes a first blade (20) and a second blade (19). The first blade (20) is set vertically, and the second blade (19) is set at an angle at the top of the first blade (20). Multiple fixed discs (16) are spaced apart on the stirring shaft (13). Each fixed disc (16) has a fixed oblique blade assembly or a composite blade assembly on its outer circumferential sidewall. Multiple external threads (14) are evenly spaced along the axis of the stirring shaft (13). The fixed disc (16) has a through hole adapted to the stirring shaft (13). The inner circumferential sidewall of the fixed disc (16) has an internal thread adapted to the external thread (14). The fixed disc (16) is fixedly connected to the stirring shaft (13) by a threaded connection, so that the position of the oblique blade assembly and the composite blade assembly on the stirring shaft (13) is adjustable to adapt to different liquid levels in the fermentation tank (1).

2. The intelligent multi-gas substrate fermentation equipment according to claim 1, characterized in that: Limiting blocks (21) are provided at the upper and lower ends of each fixed disk (16) and around the stirring shaft (13). The limiting blocks (21) are threadedly connected to the stirring shaft (13). By providing limiting blocks (21) on the upper and lower sides of the fixed disk (16), the position of the fixed disk (16) on the stirring shaft (13) is restricted.

3. The intelligent multi-gas substrate fermentation equipment according to claim 1, characterized in that: The magnetic component includes an inner magnetic ring (9) and an outer magnetic ring (8). A servo motor (7) is provided on the top of the fermentation tank (1). The outer magnetic ring (8) is fixedly provided at the output end of the servo motor (7). The inner magnetic ring (9) is fixedly provided on the top of the stirring shaft (13). The inner magnetic ring (9) and the outer magnetic ring (8) are magnetically coupled.

4. The intelligent multi-gas substrate fermentation equipment according to claim 1, characterized in that: The oblique blade assembly includes several oblique blades (18), which are inhalation blades.

5. The intelligent multi-gas substrate fermentation equipment according to claim 1, characterized in that: The stirring shaft (13) is also connected to a defoaming slurry assembly via a fixed plate (16). The defoaming slurry assembly is located above the uppermost composite blade assembly. The defoaming slurry assembly includes several defoaming slurries (12). Each defoaming slurry (12) includes a defoaming part body. Several defoaming protrusions (11) are provided on the circumferential sidewall of the defoaming part body. The shape of the defoaming protrusions (11) includes frustum, quadrangular frustum, cone and pyramidal.

6. The intelligent multi-gas substrate fermentation equipment according to claim 1, characterized in that: The fermentation tank (1) is equipped with an annular gas distributor (17) at the bottom, which is connected to the ventilation system. The annular gas distributor (17) includes a spiral disc tube connected to the air inlet pipe, and the spiral disc tube is provided with several air outlet holes, the air outlet holes facing upwards.

7. The intelligent multi-gas substrate fermentation equipment according to claim 1, characterized in that: The fermentation tank (1) includes a main tank and a temperature control jacket located outside the main tank. The temperature control jacket is filled with a heat exchange medium, which is derived from a water bath electric heater and a chiller.

8. The intelligent multi-gas substrate fermentation device according to claim 6, characterized in that: The fermentation equipment also includes a gas detection system, which includes a detection pipeline. The start and end of the detection pipeline are connected to the fermentation tank (1). A dryer (3) and a tail gas analyzer (2) are arranged in sequence on the detection pipeline according to the direction of medium flow.

9. The intelligent multi-gas substrate fermentation device according to claim 8, characterized in that: The ventilation system includes an intake pipe and an exhaust pipe. The intake pipe is connected to an H2 cylinder, an O2 cylinder, and a CO2 cylinder at its starting end, and to the fermentation tank (1) at its end. Along the fluid flow direction, a pressure reducing valve, a mass flow meter (6), a pre-filter (5), and a fine filter (4) are sequentially installed on the intake pipe. The exhaust pipe is connected to the fermentation tank (1) at its starting end, and is equipped with a reflux condenser. The liquid discharge end of the reflux condenser is connected to the fermentation tank (1), and the gas discharge end is connected to the outside.

10. The intelligent multi-gas substrate fermentation device according to claim 9, characterized in that: The fermentation tank (1) is equipped with a pressure transmitter, a temperature electrode, a defoaming electrode, a pH electrode and a DO electrode; the fermentation equipment also includes an intelligent control system, which is communicatively connected to the pressure transmitter, temperature electrode, defoaming electrode, pH electrode and DO electrode. The intelligent control system controls the valve opening of the air inlet valve and / or exhaust valve in real time based on the pressure inside the fermentation tank (1) monitored by the pressure transmitter. The intelligent control system controls the temperature of the water bath electric heating or cold water cooling in real time based on the temperature inside the fermentation tank (1) monitored by the temperature electrode. The intelligent control system controls the start and stop of the defoaming peristaltic pump in real time according to the fermentation foam monitored by the defoaming electrode, and replenishes the fermentation tank (1) with chemical defoaming agent. The intelligent control system controls the start and stop of the acid replenishment peristaltic pump or the acid replenishment and depletion peristaltic pump in real time based on the pH value in the fermentation tank (1) monitored by the pH electrode. The intelligent control system controls the power of the servo motor and the start and stop of the feed pump in real time based on the dissolved oxygen concentration in the fermentation tank (1) monitored by the DO electrode.

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