Seafood protein extraction device

Abstract

The utility model discloses a marine product protein extraction device, the inside setting 2 ultrasonic transducers of enzymolysis reaction kettle, the bottom of enzymolysis reaction kettle is connected with output pump, the rear end of output pump is connected with enzymolysis output pipe, enzymolysis output pipe is connected to low temperature separation bin, and this marine product protein extraction device improves the current marine product protein extraction and faces the condition of active loss, lipid residue, adopts double -frequency ultrasonic enzymolysis: 28kHz broken cell + 132kHz promote mass transfer can effectively reduce the enzymolysis time, adopts bionic micro -fluidic separation, can effectively remove lipoprotein, improves separation efficiency.

Description

Technical Field

[0001] This utility model relates to the field of food processing equipment technology, and in particular to a seafood protein extraction device. Background Technology

[0002] Currently, the extraction of proteins from seafood faces three major challenges: loss of activity, residual impurities, and high energy consumption. Defects of traditional hot extraction: Heat extraction at temperatures above 60℃ → protein denaturation rate > 40% (e.g., salmon collagen) Acid / alkali hydrolysis → Destroys functional peptides (80% inactivation rate of antimicrobial peptides) Low separation efficiency: Incomplete centrifugation for fat removal → Lipoprotein residue >8% (exceeding GB31636 limit) Severe membrane fouling → flux decline rate > 50% / h; CN202420027991.1 discloses a protein extraction device, including a base, a box, a support frame, a separation and collection device, a collection box, and a waste box. The support frame is fixedly installed on the box, the box is fixedly installed on the base, the waste box is installed on the box, the collection box is fixed on the base, and the separation and collection device is fixedly installed on the support frame. The separation and collection device includes a primary separation structure and a secondary separation and collection structure. This utility model relates to the field of protein extraction technology and has the following beneficial effects: the device has a graded extraction function, which makes the structure of the device simpler and easier for personnel to operate. At the same time, it improves the purity of the protein, shortens the waiting time, and improves efficiency, solving the problem that the quality of the extracted protein is not high enough due to the lack of graded extraction and separation function. Utility Model Content

[0003] Therefore, in view of the above problems, this utility model proposes a seafood protein extraction device, which solves the technical problems of activity loss and lipid residue in current seafood protein extraction.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a seafood protein extraction device, the structure of which includes an enzymatic hydrolysis reactor, an ultrasonic transducer, an output pump, an enzymatic hydrolysis output pipe, a low-temperature separation chamber, a low-temperature separation plate, a collection tank, a booster output pump, and a membrane separator. Two ultrasonic transducers are installed inside the enzymatic hydrolysis reactor. The bottom of the enzymatic hydrolysis reactor is connected to the output pump, and the rear end of the output pump is connected to the enzymatic hydrolysis output pipe. The enzymatic hydrolysis output pipe is connected to the low-temperature separation chamber. A low-temperature separation plate is installed inside the low-temperature separation chamber. The upper surface of the low-temperature separation plate is provided with V-shaped channels for lipid separation, the V-shaped channels being 50 μm deep and spaced 100 μm apart. A hydrophilic and lipophilic coating is also provided on the upper surface of the low-temperature separation plate. A collection tank is installed at the bottom of the low-temperature separation plate, comprising aqueous phase collection tanks on both sides and an oil phase collection tank in the middle. The aqueous phase collection tank is connected to the booster output pump via a pipe, and the booster output pump is connected to the membrane separator.

[0005] Furthermore, the ultrasonic transducer is fixedly connected to the inner wall of the enzymatic hydrolysis reactor via a flexible support pad, and the ultrasonic transducer adopts a structure of 28kHz+132kHz and a power density of 0.5W / mL.

[0006] Furthermore, the low-temperature separation plate is provided with 1-3 layers. When multiple layers are provided, the enzymatic hydrolysis output tube is connected to each layer through multiple branch pipes.

[0007] Furthermore, a thermoelectric cooler is provided on the back of the low-temperature separation plate to reduce the temperature. The thermoelectric cooler integrates a temperature control resistor to keep the cold end temperature at 4±0.1℃. The hot end of the thermoelectric cooler is cooled by placing heat dissipation fins in an air duct connecting multiple hot ends and using multiple high-speed fans for forced cooling.

[0008] Furthermore, the V-shaped flow channel is internally provided with a nano-groove array, the nano-grooves being 200 nm wide, 100 nm deep, and 300 nm apart, and also includes a BF4 ionic liquid modification layer with a thickness of 10 nm.

[0009] By adopting the aforementioned technical solution, the beneficial effects of this utility model are as follows: This seafood protein extraction device improves the current situation of activity loss and lipid residue in seafood protein extraction. It adopts dual-frequency ultrasonic enzymatic hydrolysis: 28kHz cell disruption + 132kHz mass transfer promotion can effectively reduce enzymatic hydrolysis time. It adopts biomimetic microfluidic separation, which can effectively remove lipoproteins and improve separation efficiency. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of the structure of this utility model; In the diagram: 1. Enzymatic hydrolysis reactor; 2. Ultrasonic transducer; 3. Output pump; 4. Enzymatic hydrolysis output pipe; 5. Low-temperature separation chamber; 6. Low-temperature separation plate; 7. Collection tank; 8. Booster output pump; 9. Membrane separator. Detailed Implementation

[0011] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments.

[0012] refer to Figure 1 This embodiment provides a seafood protein extraction device, the structure of which includes an enzymatic hydrolysis reactor 1, an ultrasonic transducer 2, an output pump 3, an enzymatic hydrolysis output pipe 4, a low-temperature separation chamber 5, a low-temperature separation plate 6, a collection tank 7, a booster output pump 8, and a membrane separator 9. Two ultrasonic transducers 2 are installed inside the enzymatic hydrolysis reactor 1. The bottom of the enzymatic hydrolysis reactor 1 is connected to the output pump 3, and the rear end of the output pump 3 is connected to the enzymatic hydrolysis output pipe 4. The enzymatic hydrolysis output pipe 4 is connected to the low-temperature separation chamber 5. A low-temperature separation plate 6 is installed inside the low-temperature separation chamber 5. The upper surface of the low-temperature separation plate 6 is provided with V-shaped channels for lipid separation, the V-shaped channels being 50 μm deep and spaced 100 μm apart. The upper surface of the low-temperature separation plate 6 is also provided with a hydrophilic-lipophobic coating. A collection tank 7 is installed at the bottom of the low-temperature separation plate 6, including aqueous phase collection tanks 7 on both sides and an oil phase collection tank 7 in the middle. The aqueous phase collection tank 7 is connected to the booster output pump 8 through a pipe, and the booster output pump 8 is connected to the membrane separator 9.

[0013] The ultrasonic transducer 2 is fixedly connected to the inner wall of the enzymatic hydrolysis reactor 1 through a flexible support pad. The ultrasonic transducer 2 adopts a structure of 28kHz+132kHz and a power density of 0.5W / mL.

[0014] The low-temperature separation plate 6 is provided with 1-3 layers. When multiple layers are provided, the enzymatic hydrolysis output tube 4 is connected to each layer through multiple branch tubes.

[0015] The back of the low-temperature separation plate 6 is provided with a semiconductor cooling chip for reducing the temperature. The semiconductor cooling chip integrates a temperature control resistor to keep the cold end temperature at 4±0.1℃. The hot end of the semiconductor cooling chip is cooled by placing heat dissipation fins in an air duct connecting multiple hot ends and using multiple high-speed fans for forced cooling.

[0016] The V-shaped flow channel is internally arranged with a nano-groove array, the nano-grooves being 200 nm wide, 100 nm deep, and spaced 300 nm apart, and also includes a BF4 ionic liquid modification layer with a thickness of 10 nm.

[0017] This seafood protein extraction device improves upon the current challenges of protein extraction from seafood, such as loss of activity and lipid residue. It employs dual-frequency ultrasonic enzymatic hydrolysis: 28kHz cell disruption + 132kHz mass transfer promotion, which effectively reduces enzymatic hydrolysis time. It also utilizes biomimetic microfluidic separation to effectively remove lipoproteins, thereby improving separation efficiency.

[0018] The liquid output from the low-temperature separation plate 6 can be fed into freeze-drying equipment to directly obtain solid protein. After drying, the protein activity retention rate is 98% (enzyme activity test).

[0019] The membrane separator 9 also includes a pulse backflush mechanism and a transmembrane differential pressure sensor for interlocking control. When the differential pressure is greater than 0.1 MPa, a 0.5 MPa pulse backflush is triggered with a response time of 0.5 s.

[0020] Radiation vacuum freeze dryers include: Infrared heating plate (wavelength 3μm) maintains protein activity; Cold trap -80℃ (silicone oil refrigerant) protects thermosensitive peptides; Protein activity retention rate: 99.2% (enzyme activity test); The membrane separator includes: Membrane module with a molecular weight cutoff of 10 kDa (301) (polyethersulfone material, pore size 5 nm) Pulse recoil mechanism (302) (pressure 0.5MPa, frequency 1Hz) Transmembrane differential pressure sensor (303) (accuracy ±0.01MPa); Protein purity ≥ 90% (SDS-PAGE assay).

[0021] Example 2 Ingredients: Frozen squid paste (18% protein content) Operating procedures: Enzymatic hydrolysis: 28kHz / 132kHz dual-frequency ultrasound (power density 0.5W / mL) Enzymatic hydrolysis at 4℃ for 1.5 hours → Cell disruption rate 99.3% (verified by electron microscopy) Separation: V-shaped flow channel (50μm depth + nanogrooves) with a flow rate of 0.8mL / min Lipoprotein separation rate: 98.7% (GC-MS detection) Membrane purification: Pressure differential 0.08 MPa (no backflush triggered) → Membrane flux maintained at 15 L / m 2 h Freeze-drying: Radiative heating (3μm wavelength) → -80℃ cold trap for water vapor capture Water activity 0.15 aw → Myosin activity 99.1% The foregoing has shown and described the basic principles, main features, and advantages of this utility model. It will be apparent to those skilled in the art that this utility model is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or basic characteristics of this utility model. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this utility model is defined by the appended claims rather than the foregoing description, and thus all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this utility model. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0022] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A seafood protein extraction device, characterized in that: Its structure includes an enzymatic hydrolysis reactor, an ultrasonic transducer, an output pump, an enzymatic hydrolysis output pipe, a low-temperature separation chamber, a low-temperature separation plate, a collection tank, a booster output pump, and a membrane separator. The enzymatic hydrolysis reactor has two ultrasonic transducers inside. The bottom of the reactor is connected to the output pump, and the rear end of the pump is connected to the enzymatic hydrolysis output pipe, which is connected to the low-temperature separation chamber. The low-temperature separation chamber has a low-temperature separation plate inside. The upper surface of the plate has V-shaped channels for lipid separation, each 50 μm deep and 100 μm apart. The upper surface also has a hydrophilic-lipophobic coating. The bottom of the plate has a collection tank, which includes aqueous phase collection tanks on both sides and an oil phase collection tank in the middle. The aqueous phase collection tank is connected to the booster output pump via a pipe, and the booster output pump is connected to the membrane separator.

2. The seafood protein extraction device according to claim 1, characterized in that: The ultrasonic transducer is fixedly connected to the inner wall of the enzymatic hydrolysis reactor via a flexible support pad. The ultrasonic transducer has a structure of 28kHz + 132kHz and a power density of 0.5W / mL.

3. The seafood protein extraction device according to claim 1, characterized in that: The low-temperature separation plate is configured with 1-3 layers. When multiple layers are configured, the enzymatic hydrolysis output tube is connected to each layer through multiple branch tubes.

4. The seafood protein extraction device according to claim 3, characterized in that: The back of the low-temperature separation plate is provided with a semiconductor cooling chip for reducing the temperature. The semiconductor cooling chip integrates a temperature control resistor to keep the cold end temperature at 4±0.1℃. The hot end of the semiconductor cooling chip is cooled by placing heat dissipation fins in an air duct connecting multiple hot ends and using multiple high-speed fans for forced cooling.

5. The seafood protein extraction device according to claim 1, characterized in that: The V-shaped flow channel is internally arranged with a nano-groove array, the nano-grooves being 200 nm wide, 100 nm deep, and spaced 300 nm apart, and also includes a BF4 ionic liquid modification layer with a thickness of 10 nm.

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