A novel cell culture well plate

Abstract

The utility model belongs to cell culture technical field, concretely points to a novel cell culture well plate, including cover and culture well plate, the inside bottom of culture well plate is equipped with a plurality of culture grooves, be equipped with first connecting plate on the inner wall of culture well plate, one end fixed connection culture groove's outside wall on first connecting plate, the lower extreme of first connecting plate is set up first through -hole, it is connected through second connecting plate between a plurality of culture grooves, the lower extreme of second connecting plate is set up second through -hole, the setting of this application through water hole, realize all need water part's water, prevent splashing baffle and reduce the splash of sterile water to culture groove, improve experimental precision, reduce the pollution probability.

Description

Technical Field

[0001] This invention belongs to the field of cell culture technology, specifically referring to a novel cell culture plate. Background Technology

[0002] Cell culture refers to a method of enabling cells to survive, grow, reproduce, and maintain their main structures and functions in vitro by simulating the in vivo environment (sterile, suitable temperature, pH, and certain nutritional conditions, etc.). Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology.

[0003] Colony-forming cell (CBC) assay, also known as colony-forming unit (CBC) assay, is currently the gold standard for in vitro detection of hematopoietic stem / progenitor cell function. Currently, colony culture typically uses cell culture plates of different sizes. Because methylcellulose medium is a semi-solid medium, sterile water needs to be added between the wells of the cell culture tank to prevent the medium from drying out during long-term culture. However, in practice, due to the large number of culture tanks, adding sterile water multiple times is cumbersome. Furthermore, adding sterile water to the gaps between the wells inevitably allows hands or pipettes to pass directly over the open end of the cell culture plate, easily causing bacterial contamination.

[0004] On the other hand, in the process of mass cell culture, multiple cell culture plates are usually stacked vertically for culture. When taking them out for observation, individual cell culture plates need to be pulled out. When this operation is carried out in the incubator, it is easy to pick the wrong layer, which will cause the lids of other culture plates to be opened and the cells to be contaminated. Utility Model Content

[0005] To address the aforementioned problems, this invention proposes a novel cell culture plate.

[0006] The technical solution adopted by this utility model is as follows: This utility model provides a novel cell culture well plate, including a cover plate and a culture well plate. The bottom of the culture well plate is provided with multiple culture tanks. A first connecting plate is provided on the inner wall of the culture well plate. One end of the first connecting plate is fixedly connected to the outer wall of the culture tank. A first connecting hole is opened at the lower end of the first connecting plate. The multiple culture tanks are connected by a second connecting plate. A second connecting hole is opened at the lower end of the second connecting plate.

[0007] Furthermore, the upper end of the inner wall of the culture plate is provided with a splash guard, and water filling holes are provided on the splash guard.

[0008] Furthermore, a retaining plate is provided on the outer wall of the culture plate, and a buckle is provided on the outer wall of the cover plate to cooperate with the retaining plate.

[0009] Furthermore, the water inlet hole is used to add sterile water.

[0010] Furthermore, the first and second connecting holes are used for the flow of sterile water in the gaps of the culture tank.

[0011] Furthermore, the splash guard is used to prevent sterile water from splashing into the culture tank.

[0012] The beneficial effects of this utility model by adopting the above structure are as follows: The water inlet holes allow water to be added to all parts that require it, while the splash guards reduce the splashing of sterile water into the culture tank, improving experimental accuracy and reducing the chance of contamination. Attached Figure Description

[0013] To more clearly illustrate the technical solution of this utility model, the drawings used in the description will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0014] Figure 1 This is a front view of a novel cell culture plate according to this utility model; Figure 2 This is a front cross-sectional view of a novel cell culture plate according to this utility model.

[0015] Figure 3 This is a top view of the culture plate.

[0016] Among them, 1. cover plate, 2. culture well plate, 3. culture tank, 4. first connecting plate, 5. first connecting hole, 6. splash baffle, 7. water filling hole, 9. second connecting plate, 10. second connecting hole, 11. buckle, 12. clamping plate. Detailed Implementation

[0017] To make the objectives, features, and advantages of this utility model more apparent and understandable, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the specific embodiments. Obviously, the embodiments described below are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this patent, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this patent.

[0018] like Figures 1-3As shown, this utility model proposes a novel cell culture plate, including a cover plate 1 and a culture plate 2. A retaining plate 12 is provided on the outer side wall of the culture plate 2, and a buckle 11 that cooperates with the retaining plate 12 is provided on the outer side wall of the cover plate 1. A splash-proof baffle 6 is provided at the upper end of the inner side wall of the culture plate 2 to prevent sterile water from splashing into the culture tank 3. A water inlet hole 7 is provided on the splash-proof baffle 6 for adding sterile water. Multiple culture tanks 3 are provided at the bottom of the inner side of the culture plate 2. A first connecting plate 4 is provided on the inner wall of the culture plate 2. One end of the first connecting plate 4 is fixedly connected to the outer side wall of the culture tank 3. A first connecting hole 5 is provided at the lower end of the first connecting plate 4. Multiple culture tanks 3 are connected by a second connecting plate 9. A second connecting hole 10 is provided at the lower end of the second connecting plate 9. The first connecting hole 5 and the second connecting hole 10 are used for sterile water to flow in the gaps between the culture tanks 3.

[0019] In practical use, open the cover plate 1, add methylcellulose culture medium and cell mixture to the culture tank 3, shake the culture plate 2 to cover the entire culture tank 3, use a syringe to draw sterile water, and slowly inject sterile water through the water inlet 7. The sterile water flows quickly along the first connecting hole 5 and the second connecting hole 10 to all parts that need sterile water. When the water level reaches about 2 / 3 of the hole diameter, stop injecting water, close the cover plate 1, and close it using the locking plate 12 and the buckle 11. Place the entire cell culture plate into the carbon dioxide incubator for culture. The above is the overall working process of this utility model. Repeat this step for the next use.

[0020] As can be seen from the above embodiments, the beneficial effects of this utility model are as follows: The water inlet holes allow water to be added to all parts that require it, while the splash guards reduce the splashing of sterile water into the culture tank, improving experimental accuracy and reducing the chance of contamination.

[0021] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A novel cell culture plate, comprising a cover plate (1) and a culture plate (2), characterized in that: The culture plate (2) has multiple culture tanks (3) at its bottom. The inner wall of the culture plate (2) has a first connecting plate (4). One end of the first connecting plate (4) is fixedly connected to the outer wall of the culture tank (3). The lower end of the first connecting plate (4) has a first connecting hole (5). The multiple culture tanks (3) are connected by a second connecting plate (9). The lower end of the second connecting plate (9) has a second connecting hole (10). The upper end of the inner wall of the culture plate (2) has a splash guard (6). The splash guard (6) has a water filling hole (7). The outer wall of the culture plate (2) has a retaining plate (12). The outer wall of the cover plate (1) has a buckle (11) that works with the retaining plate (12). The first connecting hole (5) and the second connecting hole (10) are used for the flow of sterile water in the gaps between the culture tanks (3).

Images