An auxiliary device for cell scratch experiment

Abstract

The utility model discloses a kind of auxiliary equipment for cell scratch experiment, the device includes box, hinged with box cover on box, the inside of box cover is provided with auxiliary unit, auxiliary unit includes slide, fixed groove and fixing piece;Slide is horizontally slidably connected in the inside of box cover, fixed groove is opened in the inside of slide, fixed groove array is set, fixing piece is fixedly assembled in the side of box cover, fixing piece equidistant array is set, fixing piece is used to after the horizontal movement of slide, it is fixed, the device is by setting auxiliary unit in the inside of box cover, fixed groove on slide can limit cutter, ensure that cutter always keeps perpendicular angle with petri dish in scratch process, solve the problem that cutter angle cannot be guaranteed perpendicular in existing ruler auxiliary mode, so that the depth and width of scratch are uniform, improve the accuracy of experimental result, satisfy user demand.

Description

Technical Field

[0001] This utility model relates to the field of auxiliary equipment for cell biology experiments, and in particular to an auxiliary device for cell scratch experiments. Background Technology

[0002] The cell scratch assay is a commonly used in vitro experimental method for detecting cell migration ability. The principle is to create a scratch in a culture dish containing a monolayer of cells, and then assess the cell migration ability by observing the migration of cells in the scratched area during subsequent culture.

[0003] In current cell scratch assay procedures, to ensure the straightness of the scratches, researchers usually use a ruler as an aid. Specifically, the ruler is placed against the outer wall of the culture dish, and then a cutter is used to make scratches on the monolayer of cells in the culture dish along the edge of the ruler. However, in actual operation, the researchers rely entirely on manual control of the cutter, and it is impossible to ensure that the cutter and the culture dish always maintain a perpendicular angle.

[0004] A non-perpendicular angle will result in uneven scratch depth. Scratches that are too deep in some areas will damage the cell matrix at the bottom of the culture dish, affecting normal cell growth and migration; while scratches that are too shallow in other areas will not completely sever the cells, leaving more cells in the scratched area and interfering with subsequent assessments of cell migration. Secondly, a non-perpendicular angle will cause inconsistent scratch widths, making it difficult to accurately determine the initial boundaries of the scratch when measuring cell migration distance, leading to significant errors in the measurement data and hindering the conclusion of experimental findings. Utility Model Content

[0005] The purpose of this invention is to provide an auxiliary device for cell scratch experiments to solve the problems mentioned in the background art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: An auxiliary device for cell scratch experiments, comprising a box body, a box cover hinged to the box body, an auxiliary unit provided inside the box cover, the auxiliary unit comprising a sliding plate, a fixing groove and a fixing component;

[0007] The sliding plate is horizontally connected to the inside of the box cover. The fixing groove is opened inside the sliding plate and the fixing groove is arranged in an array. The fixing member is fixedly assembled on one side of the box cover and the fixing member is arranged in an equidistant array. The fixing member is used to fix the sliding plate after it moves horizontally.

[0008] Preferably, the top of the lid has positioning holes, which are arranged in an equidistant array.

[0009] Preferably, the lid has guide grooves at both ends, and the sliding plate is slidably connected inside the guide grooves.

[0010] Preferably, one end of the skateboard is fixedly fitted with an anti-slip plate, and the other end of the skateboard is fixedly fitted with a handle.

[0011] Preferably, the box body has a positioning groove inside, and a six-well culture dish is movably engaged inside the positioning groove, with the holes on the top of the six-well culture dish corresponding to the positioning holes.

[0012] Preferably, the fixing element includes a positioning block and a plug rod;

[0013] The positioning blocks are fixedly assembled on the box cover, and the positioning blocks are arranged in an equidistant array; the insertion rod is slidably connected inside the positioning block, and the insertion rod is movably engaged inside the handle.

[0014] Preferably, the fixing member further includes a fixing seat and a spring;

[0015] The fixed base is fixedly assembled inside the positioning block, and the insertion rod is vertically slidably connected inside the fixed base; one end of the spring is fixedly assembled inside the fixed base, and the other end of the spring is fixedly assembled with the insertion rod.

[0016] Preferably, the fixing member further includes a tie rod:

[0017] The pull rod is fixedly assembled to the bottom of the insert rod, and the pull rod is vertically slidably connected inside the positioning block.

[0018] The technical effects and advantages of this utility model are as follows:

[0019] This device, by setting an auxiliary unit inside the lid, allows the fixing groove on the slide plate to limit the cutter, ensuring that the cutter always maintains a perpendicular angle to the petri dish during the scratching process. This solves the problem that the cutter angle cannot be guaranteed to be perpendicular in the existing ruler-assisted method, making the depth and width of the scratches uniform and consistent, improving the accuracy of experimental results and meeting the needs of users. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0021] Figure 2 This is a schematic diagram of the skateboard of this utility model;

[0022] Figure 3 This is a schematic diagram of the fastener of this utility model;

[0023] Figure 4 This is a schematic diagram of the six-well culture dish of this utility model.

[0024] In the diagram: 1. Box body; 2. Box lid; 3. Auxiliary unit; 31. Slide plate; 32. Fixing groove; 33. Fixing component; 331. Positioning block; 332. Insert rod; 333. Fixing base; 334. Spring; 335. Pull rod; 34. Positioning hole; 35. Guide groove; 36. Anti-detachment plate; 37. Handle; 38. Positioning groove; 4. Six-well petri dish. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] This utility model provides, for example Figure 1 , Figure 2 and Figure 4 The auxiliary device for cell scratch experiment shown includes a box body 1, a box cover 2 hinged to the box body 1, and an auxiliary unit 3 disposed inside the box cover 2. The auxiliary unit 3 includes a sliding plate 31, a fixing groove 32 and a fixing component 33.

[0027] The slide plate 31 is horizontally slidably connected to the inside of the cover 2. The fixing groove 32 is opened inside the slide plate 31 and is arranged in an array. The fixing member 33 is fixedly assembled on one side of the cover 2 and is arranged in an equidistant array. The fixing member 33 is used to fix the slide plate 31 after it moves horizontally.

[0028] Example 1: In this example, the box body 1 and the box cover 2 are hinged together to open and close. The positioning groove 38 inside the box body 1 is used to fix the six-well culture dish 4, ensuring that the culture dish does not move during the scratching process, and the top hole of the culture dish is precisely aligned with the positioning hole 34 on the box cover 2. The sliding plate 31 inside the box cover 2 can slide horizontally along the guide groove 35. The experimenter adjusts the position of the sliding plate 31 by using the handle 37. After the fixing groove 32 moves to the target scratch position, the fixing piece 33 is used to fix the sliding plate 31. At this time, the cutter is inserted into the fixing groove 32 through the positioning hole 34. The fixing groove 32 is adapted to the cutting blade and can forcibly limit the cutting angle, so that it is always perpendicular to the six-well culture dish 4. Scraping the cutter along the fixing groove 32 can form a uniform and straight scratch. At the same time, the anti-detachment plate 36 at one end of the sliding plate 31 can prevent the sliding plate 31 from falling out of the box cover 2 when sliding, ensuring the stable operation of the equipment.

[0029] like Figure 3 The auxiliary device for cell scratch experiment shown includes a box body 1, a box cover 2 hinged to the box body 1, and an auxiliary unit 3 disposed inside the box cover 2. The auxiliary unit 3 includes a sliding plate 31, a fixing groove 32 and a fixing component 33.

[0030] The slide plate 31 is horizontally slidably connected to the inside of the cover 2. The fixing groove 32 is opened inside the slide plate 31 and is arranged in an array. The fixing member 33 is fixedly assembled on one side of the cover 2 and is arranged in an equidistant array. The fixing member 33 is used to fix the slide plate 31 after it moves horizontally.

[0031] The top of the lid 2 has positioning holes 34, which are arranged in an equidistant array. Guide grooves 35 are provided at both ends of the lid 2, and a sliding plate 31 is slidably connected inside the guide grooves 35. An anti-detachment plate 36 is fixedly fitted to one end of the sliding plate 31, and a handle 37 is fixedly fitted to the other end. The interior of the box body 1 has a positioning groove 38, which movably engages a six-well culture dish 4. The holes at the top of the six-well culture dish 4 correspond to the positioning holes 34. The fixing component 33 includes a positioning block 331 and a rod 332; the positioning block 331 is fixedly fitted onto the lid 2, and the positioning blocks 331 are arranged in an equidistant array; the rod 332 is slidably connected inside the positioning block 331, and the rod 332 is movably engaged inside the handle 37. The fixing component 33 also includes a fixing seat 333 and a spring 334; the fixing seat 333 is fixedly assembled inside the positioning block 331, and the insertion rod 332 is vertically slidably connected inside the fixing seat 333; one end of the spring 334 is fixedly assembled inside the fixing seat 333, and the other end of the spring 334 is fixedly assembled to the insertion rod 332. The fixing component 33 also includes a pull rod 335: the pull rod 335 is fixedly assembled to the bottom of the insertion rod 332, and the pull rod 335 is vertically slidably connected inside the positioning block 331.

[0032] Example 2: In this example, the positioning block 331 is fixed to the cover 2 with bolts. The fixing seat 333 inside it is used to limit the vertical sliding trajectory of the insertion rod 332 and prevent the insertion rod 332 from deviating. One end of the spring 334 is fixed in the fixing seat 333, and the other end is connected to the insertion rod 332. In the natural state, the elastic force of the spring 334 will push the insertion rod 332 to move upward, so that its top end is locked into the slot of the handle 37, thereby fixing the slide plate 31 in the current position. When it is necessary to adjust the position of the slide plate 31, the experimenter pulls the pull rod 335 downward. The pull rod 335 drives the insertion rod 332 to slide downward against the elastic force of the spring 334, so that the insertion rod 332 is disengaged from the slot of the handle 37. At this time, the handle 37 can be pulled freely to adjust the slide plate 31. After the adjustment is completed, the pull rod 335 is released, the spring 334 resets and pushes the insertion rod 332 to lock into the slot again, thereby re-fixing the slide plate 31.

[0033] Finally, it should be noted that the above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. An auxiliary device for cell scratch assay, comprising a housing (1) with a lid (2) hinged to the housing (1), characterized in that, An auxiliary unit (3) is provided inside the cover (2), the auxiliary unit (3) including a sliding plate (31), a fixing groove (32) and a fixing member (33): The slide plate (31) is horizontally slidably connected to the inside of the cover (2). The fixing groove (32) is opened inside the slide plate (31). The fixing groove (32) is arranged in an array. The fixing member (33) is fixedly assembled on one side of the cover (2). The fixing member (33) is arranged in an equidistant array. The fixing member (33) is used to fix the slide plate (31) after it moves horizontally.

2. The auxiliary device for cell scratch assay according to claim 1, characterized in that, The top of the lid (2) is provided with positioning holes (34), which are arranged in an equidistant array.

3. The auxiliary device for cell scratch assay according to claim 1, characterized in that, The lid (2) has guide grooves (35) at both ends, and the slide plate (31) is slidably connected inside the guide grooves (35).

4. The auxiliary device for cell scratch assay according to claim 1, characterized in that, One end of the skateboard (31) is fixedly fitted with an anti-slip plate (36), and the other end of the skateboard (31) is fixedly fitted with a handle (37).

5. The auxiliary device for cell scratch assay according to claim 2, characterized in that, The box body (1) has a positioning groove (38) inside, and a six-well culture dish (4) is movably engaged inside the positioning groove (38). The holes on the top of the six-well culture dish (4) correspond to the positioning holes (34).

6. The auxiliary device for cell scratch assay according to claim 4, characterized in that, The fastener (33) includes: Positioning blocks (331) are fixedly mounted on the box cover (2), and the positioning blocks (331) are arranged in an equidistant array; The insertion rod (332) is slidably connected inside the positioning block (331), and the insertion rod (332) is movably engaged inside the handle (37).

7. The auxiliary device for cell scratch assay according to claim 6, characterized in that, The fastener (33) also includes: The fixed base (333) is fixedly assembled inside the positioning block (331), and the insertion rod (332) is vertically slidably connected inside the fixed base (333); A spring (334) is fixedly assembled at one end inside a fixed base (333), and the other end of the spring (334) is fixedly assembled with a plug rod (332).

8. The auxiliary device for cell scratch assay according to claim 6, characterized in that, The fastener (33) also includes: A pull rod (335) is fixedly mounted on the bottom of the insert rod (332), and the pull rod (335) is vertically slidably connected inside the positioning block (331).

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