A fully automatic biological culture device

Abstract

The utility model provides a kind of full-automatic biological culture device, including culture bin, the culture bin front side is equipped with bin mouth, the culture bin inside rear side is equipped with multilayer incubation support plate, the culture bin inside is respectively equipped with first pick-and-place mechanism for moving culture plate from bin mouth into bin or moving out to bin outside, second pick-and-place mechanism for taking culture plate from incubation support plate or placing on incubation support plate, water adding tray for containing water, heating sheet for heating and ultraviolet lamp for providing illumination, the culture bin side wall is respectively equipped with air outlet, air inlet and hot air machine, and the hot air machine input end is located in culture bin inside. The utility model provides stable, sterile and controllable growth environment for biological sample, and greatly reduces the degree of manual participation, meets the demand of biological culture in scientific research, medical treatment, industrial production and other scenes.

Description

Technical Field

[0001] This utility model relates to the field of biological culture equipment technology, specifically to a fully automated biological culture device. Background Technology

[0002] In fields such as bioengineering and medical testing, biological culture is frequently involved. Traditional biological culture devices, such as ordinary incubators, have several limitations: First, traditional biological culture methods suffer from poor precision in controlling environmental parameters, making it difficult to maintain the constant conditions required for culture. Second, the culture process relies on manual operation, which is prone to introducing errors and contamination risks, and manual operation is inefficient when large-scale culture is required. Traditional biological culture methods have significant shortcomings in terms of the precision of environmental parameter control and culture efficiency, making it difficult to guarantee the effective conduct of biological culture and the accuracy of culture results. Utility Model Content

[0003] To address the problems in the background art, this utility model proposes a fully automated biological culture device, including a culture chamber. The culture chamber has an opening on its front side and multiple incubation trays on its rear side. The culture chamber is equipped with a first pick-and-place mechanism for moving the culture trays from the opening into the chamber or out of the chamber, a second pick-and-place mechanism for removing the culture trays from or placing them on the incubation trays, a water tray for holding liquid water, a heating element for heating, and an ultraviolet lamp for providing illumination. The side walls of the culture chamber are equipped with an air outlet, an air inlet, and a hot air blower, with the input end of the hot air blower located inside the culture chamber. Preferably, it also includes a main control board, which is electrically connected to the hot air blower, the ultraviolet lamp, the first pick-and-place mechanism, the second pick-and-place mechanism, and the heating element.

[0004] Preferably, the first pick-and-place mechanism includes a first vertical guide rail and a first track seat. The first vertical guide rail is fixed to one side of the incubation tray, the first track seat is slidably disposed on the first vertical guide rail, and a tray is slidably connected to the first track seat. The sliding direction of the tray is the front-to-back direction. The first track seat is driven by a first vertical drive mechanism, and the tray is driven by a first horizontal drive mechanism. The first vertical drive mechanism includes a first linear motor, which is fixed inside the culture chamber, and the lead screw nut of the first linear motor is fixedly connected to the first track seat; The first horizontal drive mechanism includes a first stepper motor, a first drive shaft, and a first rack. The first stepper motor is fixed inside the culture chamber, the first drive shaft is rotatably connected inside the culture chamber, the output end of the first stepper motor is connected to the first drive shaft, a first gear is fixed on the first drive shaft, the first gear meshes with the first rack, the first rack is slidably connected to the first track seat, and the support plate is fixedly connected to the first rack.

[0005] Preferably, the second pick-and-place mechanism includes a second vertical guide rail and a second track seat. The second vertical guide rail is fixed to the side of the incubation tray away from the first pick-and-place mechanism. The second track seat is slidably disposed on the second vertical guide rail. A push plate is slidably connected to the second track seat. The push plate slides in the front-to-back direction. The second track seat is driven by a second vertical drive mechanism, and the push plate is driven by a second horizontal drive mechanism. The second vertical drive mechanism includes a second linear motor, which is fixed inside the culture chamber, and the lead screw nut of the second linear motor is fixedly connected to the second track seat; The second horizontal drive mechanism includes a second stepper motor, a second drive shaft, and a second rack. The second stepper motor is fixed inside the culture chamber, the second drive shaft is rotatably connected inside the culture chamber, the output end of the second stepper motor is connected to the second drive shaft, a second gear is fixed on the second drive shaft, the second gear meshes with the second rack, the second rack is slidably connected to the second track seat, and the push plate is fixedly connected to the second rack.

[0006] Preferably, a plurality of first eccentric guide wheels are rotatably connected to the first track seat, and the plurality of first eccentric guide wheels are respectively located on the upper and lower sides of the first rack and in contact with the first rack; The second track seat is rotatably connected to a plurality of second eccentric guide wheels, which are located on the upper and lower sides of the second rack and in contact with the second rack.

[0007] Preferably, a heating plate is fixed to the rear side of the culture chamber, the incubation tray is fixed to the front side of the heating plate, the heating element is fixed to the front side of the heating plate, an upper cover is fixed to the top of the heating plate, the top of the upper cover is connected to the top plate of the culture chamber through an isolation column, and the top of the top plate of the culture chamber is connected to the main control board through heat insulation cotton.

[0008] Preferably, the culture chamber is equipped with a carbon dioxide sensor, an oxygen sensor, and a temperature sensor, and the main control board is electrically connected to the carbon dioxide sensor, the oxygen sensor, and the temperature sensor, respectively.

[0009] Preferably, an observation window is provided on one side of the culture chamber.

[0010] Preferably, the water filling tray is located below the incubation tray, and a pull-out opening is provided on one side of the culture chamber, through which the water filling tray is slidably disposed within the culture chamber.

[0011] Preferably, the air inlet is provided with an inner filter box, the inner filter box contains a filter, and the air inlet is connected to the interior of the inner filter box; the air outlet is provided with an air outlet filter cover.

[0012] The beneficial effects of this utility model are as follows: This invention features a heating element, a water tray, a UV lamp, an air inlet, and a hot air blower, enabling it to simulate specific environmental conditions required for biological growth, such as temperature, humidity, gas, and light, with precise and controllable environmental parameters. Furthermore, it includes a loading and unloading device that can move culture plates containing biological samples into the culture chamber and place them on an incubation tray, and can also remove culture plates that have completed cultivation from the incubation tray and move them outside the culture chamber. This allows for the movement of multiple culture plates into or out of the culture chamber while minimizing exposure to the culture environment, thus reducing the possibility of environmental contamination.

[0013] This invention provides a stable, sterile, and controllable growth environment for biological samples, and greatly reduces the degree of human intervention, meeting the needs of biological culture in scientific research, medical treatment, industrial production and other scenarios. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of the present utility model. Figure One ; Figure 2 This is a schematic diagram of the overall structure of the present utility model. Figure Two ; Figure 3 This is a schematic diagram of the internal structure of the culture chamber of this utility model. Figure One ; Figure 4 This is a schematic diagram of the internal structure of the culture chamber of this utility model. Figure Two ; Figure 5 This is a schematic diagram of the internal structure of the culture chamber of this utility model. Figure Three ; Figure 6 This is a schematic diagram of the structure of the first and second track seats of this utility model.

[0015] The diagram is labeled as follows: 1. Culture chamber; 2. Chamber opening; 3. Chamber door; 4. Observation window; 5. Air outlet filter; 6. Pipe joint; 7. Hot air blower; 8. Solenoid valve; 9. First vertical guide rail; 10. First track seat; 11. Support plate; 12. First linear motor; 13. First stepper motor; 14. First drive shaft; 15. First rack; 16. Second vertical guide rail; 17. Second track seat; 18. Push plate; 19. Second linear motor; 20. Second stepper motor; 21. Second drive shaft; 22. Second rack; 23. Second eccentric guide wheel; 24. Adjustable guide seat; 25. Incubation tray; 26. Heating plate; 27. Heating element; 28. Isolation column; 29. ​​Culture plate; 30. Top cover; 31. Insulation cotton; 32. Main control board; 33. Carbon dioxide sensor; 34. Oxygen sensor; 35. Temperature sensor; 36. First eccentric guide wheel; 37. Water filling tray; 38. Inner filter box; 39. Box cover; 40. EVA side panel; 41. Clamping plate; 42. Foot pad; 43. Aviation plug; 44. Ultraviolet lamp; 45. Ballast; 46. Door frame pressure plate; 47. Incubator top plate. Detailed Implementation

[0016] To make this utility model clearer and more understandable, the technical solution of this utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the given embodiments are only one of the implementation methods and do not represent all embodiments.

[0017] In this article, terms such as "inner," "outer," "upper," "lower," "front," "back," "left," and "right" are established based on the positional relationships shown in the attached figures. Depending on the attached figures, the corresponding positional relationships may also change. Therefore, they should not be interpreted as an absolute limitation on the scope of protection.

[0018] Combined with appendix Figure 1 -Appendix Figure 6 A fully automated biological culture device includes a culture chamber 1 with an opening 2 at its front and multiple incubation trays 25 on its rear side. The culture chamber 1 is equipped with a first loading / unloading mechanism for moving culture trays 29 from the opening 2 into or out of the chamber, a second loading / unloading mechanism for removing or placing culture trays 29 from or onto the incubation trays 25, a water tray 37 for holding liquid water, a heating element 27 for heating, and an ultraviolet lamp 44 for providing illumination. The side walls of the culture chamber 1 are equipped with an air outlet, an air inlet, and a hot air blower 7, with the input end of the hot air blower 7 located inside the culture chamber 1. The air inlet introduces the gas required for biological culture, and the air outlet discharges the gas from the culture chamber 1, achieving gas circulation within the chamber. The hot air blower 7 promotes gas flow within the culture chamber 1.

[0019] Specifically, a door 3 is provided at the opening 2 of the culture chamber. The door 3 is connected to the culture chamber 1 by a torsion spring, and the opening 2 of the culture chamber is opened or closed by the door 3.

[0020] Specifically, it also includes a main control board 32, which is electrically connected to the hot air blower 7, the ultraviolet lamp 44, the first pick-and-place mechanism, the second pick-and-place mechanism, and the heating element 27. The main control board 32 controls the operating parameters of each component. More specifically, the ultraviolet lamp 44 is connected to a ballast 45, which coordinates the control of the ultraviolet lamp 44.

[0021] Specifically, the first pick-and-place mechanism includes a first vertical guide rail 9 and a first track seat 10. The first vertical guide rail 9 is fixed to one side of the incubation tray 25. The first track seat 10 is slidably disposed on the first vertical guide rail 9. A tray 11 is slidably connected to the first track seat 10. The sliding direction of the tray 11 is the front-to-back direction. The first track seat 10 is driven by a first vertical drive mechanism, and the tray 11 is driven by a first horizontal drive mechanism. The first vertical drive mechanism includes a first linear motor 12, which is fixed inside the culture chamber 1, and the lead screw nut of the first linear motor 12 is fixedly connected to the first track seat 10. The first horizontal drive mechanism includes a first stepper motor 13, a first drive shaft 14, and a first rack 15. The first stepper motor 13 is fixed inside the culture chamber 1. The first drive shaft 14 is rotatably connected inside the culture chamber 1. The output end of the first stepper motor 13 is connected to the first drive shaft 14. A first gear is fixed on the first drive shaft 14. The first gear meshes with the first rack 15. The first rack 15 is slidably connected to the first track seat 10. The support plate 11 is fixedly connected to the first rack 15.

[0022] The second pick-and-place mechanism includes a second vertical guide rail 16 and a second track seat 17. The second vertical guide rail 16 is fixed to the side of the incubation tray 25 away from the first pick-and-place mechanism. The second track seat 17 is slidably disposed on the second vertical guide rail 16. A push plate 18 is slidably connected to the second track seat 17. The push plate 18 slides in the front-to-back direction. The second track seat 17 is driven by a second vertical drive mechanism, and the push plate 18 is driven by a second horizontal drive mechanism. The second vertical drive mechanism includes a second linear motor 19, which is fixed inside the culture chamber 1, and the lead screw nut of the second linear motor 19 is fixedly connected to the second track seat 17. The second horizontal drive mechanism includes a second stepper motor 20, a second transmission shaft 21, and a second rack 22. The second stepper motor 20 is fixed inside the culture chamber 1, and the second transmission shaft 21 is rotatably connected inside the culture chamber 1. The output end of the second stepper motor 20 is connected to the second transmission shaft 21. A second gear is fixed on the second transmission shaft 21. The second gear meshes with the second rack 22. The second rack 22 is slidably connected to the second track seat 17. The push plate 18 is fixedly connected to the second rack 22.

[0023] When the first linear motor 12 is activated, the lead screw nut of the first linear motor 12 moves up and down, thereby driving the first track seat 10 to move up and down along the first vertical guide rail 9. The first track seat 10 drives the tray 11 to move up and down, so that the tray 11 can move the culture plate 29 to the incubation tray 25 where it is to be placed. When the first stepper motor 13 is activated, it drives the first transmission shaft 14 to rotate. The first transmission shaft 14 drives the first gear to rotate. The first gear drives the first rack 15 it meshes with to move back and forth, moving the tray 11 closer to or away from the opening 2. Similarly, the second linear motor 19 drives the pusher plate 18 to move up and down, and the second stepper motor 20 drives the pusher plate 18 to move back and forth, so that the pusher plate 18 moves closer to or away from the incubation tray 25.

[0024] During cultivation, the first stepper motor 13 drives the tray 11 to move from the opening 2 to outside the cultivation chamber 1. The staff places the cultivation plate 29 on the tray 11. After placement, the first stepper motor 13 drives the tray 11 back into the chamber. The first linear motor 12 drives the tray 11 with the cultivation plate 29 to move up or down, moving it to the position above the front side of the incubation tray 25 to be placed. The second stepper motor 20 and the second linear motor 19 drive the pusher 18 to be located in front of and / or behind the cultivation plate 29. Then, the second stepper motor 20 drives the pusher 18 to move backward, pushing the cultivation plate 29 onto the incubation tray 25.

[0025] More specifically, spring plates are provided on the left and right sides of the tray 11, and clamping plates 41 are fixed on the spring plates. When a culture plate 29 is placed on the tray 11, the two clamping plates 41 clamp the culture plate 29 under the elastic force of the spring plates, thereby improving the stability of the culture plate 29 placed on the tray 11.

[0026] The first drive shaft 14 and the second drive shaft 21 can be splined drive shafts, and the first gear and the second gear can be splined gear pairs, which are pressed onto the track seat via an oil reservoir. A stepper motor can be connected to the drive shaft via a pulley mechanism. The output end of the stepper motor is connected to the driving pulley, and one end of the drive shaft is connected to the driven pulley. The driving pulley and the driven pulley are connected via a synchronous belt. The two stepper motors and the two linear motors are electrically connected to the main control board 32.

[0027] Specifically, a plurality of first eccentric guide wheels 36 are rotatably connected to the first track seat 10. The plurality of first eccentric guide wheels 36 are located on the upper and lower sides of the first rack 15 and are in contact with the first rack 15. Multiple second eccentric guide wheels 23 are rotatably connected to the second track seat 17. These second eccentric guide wheels 23 are located on the upper and lower sides of the second rack 22 and are in contact with it. The eccentric structure of the eccentric guide wheels can adaptively compensate for mis-measurements through slight rotation, avoiding jamming or damage caused by rigid contact. This provides tension and guidance for the rack's sliding, improving its sliding effect. More specifically, the toothed side of the rack is located within the track seat, and through slots are provided above and below the track seat. The eccentric guide wheels are connected to the track seat via adjustable guide seats 24, and the eccentric guide wheels contact the rack through these slots. The position of the adjustable guide seats 24 can be adjusted vertically. For example, the adjustable guide seats 24 can be bolted to the track seat, and the vertical adjustment between the adjustable guide seats 24 and the track seat can be achieved by reducing or adding shims. The contact pressure between the eccentric guide wheels and the rack can be adjusted by adjusting the position of the adjustable guide seats 24, thereby adjusting the tension. The position adjustment method of the adjustable guide seat 24 is not limited to the above scheme. As long as the position adjustment effect can be achieved, it will not be elaborated here.

[0028] To prevent the rack from detaching from the track seat, baffles are provided at both ends of the first rack 15 and the second rack 22.

[0029] Specifically, a heating plate 26 is fixed to the rear side of the culture chamber 1, an incubation tray 25 is fixed to the front side of the heating plate 26, a heating element 27 is fixed to the front side of the heating plate 26, and a top cover plate 30 is fixed to the top of the heating plate 26. The top of the top cover plate 30 is connected to the top plate of the culture chamber 47 via an isolation column 28, and the top of the top plate of the culture chamber 47 is connected to the main control board 32 via heat insulation cotton 31. The heat insulation cotton 31 and the isolation column 28 can reduce the heat transfer effect of the heating element 27 and prevent the main control board 32 from overheating.

[0030] More specifically, the culture chamber 1 is equipped with a carbon dioxide sensor 33, an oxygen sensor 34, and a temperature sensor 35. The main control board 32 is electrically connected to the carbon dioxide sensor 33, the oxygen sensor 34, and the temperature sensor 35, respectively. The carbon dioxide sensor 33, the oxygen sensor 34, and the temperature sensor 35 detect the carbon dioxide content, oxygen content, and temperature within the chamber, improving the accuracy of the culture environment conditions. More specifically, the carbon dioxide sensor 33 and the oxygen sensor 34 are mounted on the top plate 47 of the incubator, and the temperature sensor 35 is mounted on the heating plate 26.

[0031] Two stepper motors and two linear motors are fixed on the top plate 47 of the incubator. The lead screw of the linear motor is rotatably connected to the bottom plate of the culture chamber 1. The two ends of the two drive shafts are rotatably connected to the top plate 47 of the incubator and the bottom plate of the culture chamber 1, respectively.

[0032] Specifically, an observation window 4 is provided on one side of the culture chamber 1, through which the condition inside the culture chamber 1 can be observed, facilitating monitoring of the culture process. The observation window 4 can be made of quartz glass. More specifically, a window is opened on the culture chamber 1, and a door frame plate is fixed to the outer wall of the culture chamber 1 around the window. The quartz glass is fixed to the door frame plate by a door frame pressure plate 46, and a hollow silicone sealing strip is provided between the quartz glass and the door frame plate and / or the door frame pressure plate 46.

[0033] Specifically, the water-adding tray 37 is located below the incubation tray 25, and a pull-out opening is provided on one side of the culture chamber 1. The water-adding tray 37 is slidably disposed inside the culture chamber 1 through the pull-out opening. The water-adding tray 37 can be pulled into or pulled out of the culture chamber 1, facilitating the addition or replacement of water.

[0034] Specifically, the air inlet is equipped with an inner filter box 38, which contains a filter. The air inlet is connected to the interior of the inner filter box 38, and the filter filters the gas entering the culture chamber 1. The air outlet is equipped with an outlet filter cover 5 to prevent external pollutants from entering the culture chamber 1. More specifically, the air inlet is equipped with a pipe connector 6. The pipe connector 6 is connected to a pipe outside the culture chamber 1, and the other end of the pipe connector 6 is connected to the inner filter box 38. The pipe connector 6 facilitates the connection of a gas pipe for supplying air into the chamber. The gas pipe is connected to a solenoid valve 8, which controls the opening or closing of the gas pipe. Multiple air inlets can be provided to supply different gases required for culture. The various gases are filtered and mixed by the inner filter box 38 before entering the culture chamber 1. More specifically, the inner filter box 38 is open at one end and located outside the culture chamber 1. The open end of the inner filter box 38 is detachably connected to the inner filter box 38 by a cover 39 via a magnet embedded in the cover, so as to open or close the inner filter box 38, which facilitates the maintenance or replacement of the filter.

[0035] Specifically, the outer wall of the culture chamber 1 is provided with an aerogel plate, which provides protection and temperature maintenance for the culture chamber 1. An EVA (ethylene-vinyl acetate copolymer) side patch 40 is provided on the outer wall of the culture chamber 1 at the pipe joint 6. An aviation plug 43 is provided on the top side wall of the culture chamber 1 for integrating and connecting the power lines of the electrical components inside the culture chamber 1.

[0036] Sealing rings are provided at the connections of the water inlet tray 37 and the culture chamber 1, the inner filter box 38 and the culture chamber 1, the air outlet filter 5 and the culture chamber 1, and the hot air blower 7 and the culture chamber 1. The blades of the hot air blower 7 are made of aluminum alloy. A foot pad 42 is fixed to the bottom of the culture chamber 1.

[0037] Although embodiments of the present invention have been shown and described, those skilled in the art will be able to make various changes, modifications, substitutions and alterations to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A fully automated biological culture device, characterized in that: The system includes a culture chamber (1), with a vent (2) on the front side of the culture chamber (1) and a multi-layer incubation tray (25) on the rear side of the culture chamber (1). The culture chamber (1) is equipped with a first pick-and-place mechanism for moving the culture plate (29) from the vent (2) into the chamber or out of the chamber, a second pick-and-place mechanism for removing the culture plate (29) from the incubation tray (25) or placing it on the incubation tray (25), a water filling tray (37) for holding liquid water, a heating element (27) for heating, and an ultraviolet lamp (44) for providing light. The side wall of the culture chamber (1) is equipped with an air outlet, an air inlet, and a hot air blower (7). The input end of the hot air blower (7) is located inside the culture chamber (1).

2. The fully automated biological culture device according to claim 1, characterized in that: It also includes a main control board (32), which is electrically connected to the hot air blower (7), the ultraviolet lamp (44), the first pick-up and place mechanism, the second pick-up and place mechanism and the heating element (27).

3. The fully automated biological culture device according to claim 2, characterized in that: The first pick-and-place mechanism includes a first vertical guide rail (9) and a first track seat (10). The first vertical guide rail (9) is fixed to one side of the incubation tray (25). The first track seat (10) is slidably disposed on the first vertical guide rail (9). A tray (11) is slidably connected to the first track seat (10). The sliding direction of the tray (11) is the front-to-back direction. The first track seat (10) is driven by a first vertical drive mechanism, and the tray (11) is driven by a first horizontal drive mechanism. The first vertical drive mechanism includes a first linear motor (12), which is fixed inside the culture chamber (1), and the lead screw nut of the first linear motor (12) is fixedly connected to the first track seat (10); The first horizontal drive mechanism includes a first stepper motor (13), a first transmission shaft (14), and a first rack (15). The first stepper motor (13) is fixed inside the culture chamber (1). The first transmission shaft (14) is rotatably connected inside the culture chamber (1). The output end of the first stepper motor (13) is connected to the first transmission shaft (14). A first gear is fixed on the first transmission shaft (14). The first gear meshes with the first rack (15). The first rack (15) is slidably connected to the first track seat (10). The tray (11) is fixedly connected to the first rack (15).

4. The fully automated biological culture device according to claim 3, characterized in that: The second pick-and-place mechanism includes a second vertical guide rail (16) and a second track seat (17). The second vertical guide rail (16) is fixed to the side of the incubation tray (25) away from the first pick-and-place mechanism. The second track seat (17) is slidably disposed on the second vertical guide rail (16). A push plate (18) is slidably connected to the second track seat (17). The push plate (18) slides in the front-back direction. The second track seat (17) is driven by a second vertical drive mechanism, and the push plate (18) is driven by a second horizontal drive mechanism. The second vertical drive mechanism includes a second linear motor (19), which is fixed inside the culture chamber (1), and the lead screw nut of the second linear motor (19) is fixedly connected to the second track seat (17); The second horizontal drive mechanism includes a second stepper motor (20), a second transmission shaft (21), and a second rack (22). The second stepper motor (20) is fixed inside the culture chamber (1), and the second transmission shaft (21) is rotatably connected inside the culture chamber (1). The output end of the second stepper motor (20) is connected to the second transmission shaft (21). A second gear is fixed on the second transmission shaft (21). The second gear meshes with the second rack (22). The second rack (22) is slidably connected to the second track seat (17). The push plate (18) is fixedly connected to the second rack (22).

5. The fully automated biological culture device according to claim 4, characterized in that: Multiple first eccentric guide wheels (36) are rotatably connected to the first track seat (10). The multiple first eccentric guide wheels (36) are located on the upper and lower sides of the first rack (15) and are in contact with the first rack (15). The second track seat (17) is rotatably connected to a plurality of second eccentric guide wheels (23), which are located on the upper and lower sides of the second rack (22) and in contact with the second rack (22).

6. The fully automated biological culture device according to claim 2, characterized in that: A heating plate (26) is fixed to the rear side of the culture chamber (1). The incubation tray (25) is fixed to the front side of the heating plate (26). The heating element (27) is fixed to the front side of the heating plate (26). A top cover plate (30) is fixed to the top of the heating plate (26). The top of the top cover plate (30) is connected to the top plate of the culture chamber (47) through an isolation column (28). The top of the top plate of the culture chamber (47) is connected to the main control board (32) through heat insulation cotton (31).

7. The fully automated biological culture device according to claim 2, characterized in that: The culture chamber (1) is equipped with a carbon dioxide sensor (33), an oxygen sensor (34) and a temperature sensor (35), and the main control board (32) is electrically connected to the carbon dioxide sensor (33), the oxygen sensor (34) and the temperature sensor (35) respectively.

8. The fully automated biological culture device according to claim 1, characterized in that: The culture chamber (1) is provided with an observation window (4) on one side.

9. The fully automated biological culture device according to claim 1, characterized in that: The water filling tray (37) is located below the incubation tray (25), and a pull-out opening is provided on one side of the culture chamber (1). The water filling tray (37) is slidably placed inside the culture chamber (1) through the pull-out opening.

10. The fully automated biological culture device according to claim 1, characterized in that: An inner filter box (38) is provided at the air inlet, and a filter is provided inside the inner filter box (38). The air inlet is connected to the inside of the inner filter box (38); an air outlet filter cover (5) is provided at the air outlet.

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