Assemblable reusable multi-functional qu echers purification tube

Abstract

The utility model discloses a kind of multifunctional QuEChERS purification tubes of assembly and reusability, comprising: at least two hollow tubes, the connecting port is set in both ends of the hollow tube;Cover, the cover detachably connects connecting port to close the one end of hollow tube;Collecting tube, the collecting tube is formed collecting port and is set on;Filter unit, the filter unit two ends are connected with two hollow tubes corresponding connecting port respectively;Or, the filter unit two ends are connected with collecting port and connecting port respectively. The filter unit is set to realize the filtration separation of sample in hollow tube, to facilitate the filtration separation of viscous extract, the filtration mode of filter unit can be separated and transferred by centrifugation to supernatant, so as to ensure the accuracy of test, to reduce use cost.

Description

Technical Field

[0001] This utility model relates to the field of testing technology for agricultural products, aquatic products, food, and Chinese medicinal materials, and more specifically to a multifunctional QuEChERS purification tube that can be assembled and reused. Background Technology

[0002] QuEChERS is a rapid sample pretreatment technique used for the detection of agricultural products, aquatic products, food, and traditional Chinese medicine. Current QuEChERS methods include the following steps:

[0003] 1. Place the sample to be tested into a centrifuge tube, add the extraction solution and place it in a vortex mixer for vortexing. After vortexing, centrifuge the sample and transfer the supernatant to another centrifuge tube.

[0004] 2. Add salt reagent to the centrifuge tube, vortex and centrifuge in sequence, and transfer the supernatant to another centrifuge tube.

[0005] 3. Add the purification reagent to the centrifuge tube, perform vortexing and centrifugation in sequence, filter the supernatant and transfer it to another centrifuge tube.

[0006] 4. Place the centrifuge tube in a nitrogen blower for concentration. After concentration, add the reconstitution solution and place it in a vortex mixer for vortex reconstitution. After reconstitution, centrifuge and filter the supernatant into a sample vial for testing.

[0007] In the above operations, the supernatant is usually transferred by direct pouring. This method can lead to liquid residues such as those adsorbed onto the centrifuge tube, resulting in inaccurate results. Therefore, internal standard reagents are required for accuracy calibration, which makes the process cumbersome and increases costs. In addition, if the sample extract is viscous, it is very difficult to manually filter it using a conventional syringe filter head. Therefore, this method needs to be improved. Utility Model Content

[0008] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide a multifunctional QuEChERS purification tube that can be assembled and reused, so as to solve the above-mentioned technical problems.

[0009] To achieve the above objectives, the present invention provides the following technical solution:

[0010] The assembleable and reusable multi-functional QuEChERS purification tube includes:

[0011] At least two hollow tubes, each with a connection port at both ends;

[0012] A cover body, wherein the cover body is detachably connected to a connection port to seal one end of the hollow tube;

[0013] A collection tube, wherein a collection port is formed on the collection tube;

[0014] A filter unit, wherein two hollow tubes are respectively connected to the corresponding connection ports at both ends of the filter unit;

[0015] Alternatively, the two ends of the filter unit are connected to a collection port and a connection port, respectively.

[0016] As a further improvement of this utility model, two hollow tubes are respectively inserted into the two ends of the filter unit.

[0017] As a further improvement of this utility model, the filter unit is provided with sealing rings at both ends. When the filter unit is inserted into the connection port, the sealing rings are used to seal the gap between the filter unit and the connection port.

[0018] As a further improvement of this utility model, the collection port / connection port is threadedly connected to the filter unit.

[0019] As a further improvement of this utility model, the filter unit includes a filter base, the filter base is provided with a through filter hole, the filter hole is provided with a filter element, and both ends of the filter base are provided with connecting sections, the connecting sections are used to connect to a connection port or a collection port.

[0020] As a further improvement of this utility model, the filter element pore size includes 0.4um, 0.2um and 0.1um.

[0021] As a further improvement of this utility model, the diameters of the connecting ports at both ends of the hollow tube are different, and the number of the covers is at least 2, with each of the two covers being adapted to one of the two connecting ports.

[0022] As a further improvement of this utility model, the diameter of the hollow tube gradually decreases from one end of the connection port to the other end of the connection port.

[0023] As a further improvement of this utility model, the two connecting ports with different diameters of the hollow tubes can be nested together.

[0024] As a further improvement of this utility model, the filter unit is integrally connected with the hollow tube.

[0025] The beneficial effects of this utility model are:

[0026] 1. The filter unit enables the filtration and separation of samples inside the hollow tube, facilitating the direct filtration and separation of extracts with high viscosity, thus solving the problem of the difficulty in manually filtering high-viscosity extracts.

[0027] 2. The filtration unit enables automatic separation and transfer of the supernatant through centrifugation, avoiding the uncertainty of manual operation, reducing the use of internal standard reagents, thus reducing the cost and improving the accuracy of the experiment.

[0028] 3. The separate design of the hollow tube and the filter unit facilitates the cleaning of the purification tube, which can be reused, further reducing the cost of use;

[0029] 4. The purification tube of this patent can complete the extraction, salting out, purification and collection of samples in one complete assembly operation, which greatly simplifies the experimental steps and shortens the experimental operation time.

[0030] 5. By setting specific centrifugal force (or centrifuge speed) and filter units with different pore sizes, automatic filtration of liquids in different steps can be achieved, while preventing liquids from entering the next hollow tube. Attached Figure Description

[0031] Figure 1 This is a schematic diagram of the first embodiment of the present utility model;

[0032] Figure 2 This is a schematic cross-sectional view of the connection between the empty tube and the collecting tube in the first embodiment of this utility model;

[0033] Figure 3 This is a schematic cross-sectional view of the connection between the empty tube and the collecting tube in the second embodiment of this utility model;

[0034] Figure 4 This is a schematic diagram of the hollow tube in the third embodiment of this utility model;

[0035] Figure 5 This is a schematic diagram of the hollow tube connection in the fourth embodiment of this utility model;

[0036] Figure 6 This is a schematic diagram of the hollow tube connection in the fifth embodiment of this utility model.

[0037] Reference numerals: 1. Hollow tube; 2. Connection port; 3. Cover; 4. Collection tube; 5. Collection port; 6. Filter unit; 7. Sealing ring; 8. Filter base; 9. Filter hole; 10. Filter element; 11. Connection section. Detailed Implementation

[0038] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Identical components are indicated by the same reference numerals.

[0039] Figure 1-2 In the first embodiment of this utility model, the assembleable and reusable multifunctional QuEChERS purification tube includes: at least two hollow tubes 1, each of which has a connection port 2 at both ends. In this embodiment, the number of hollow tubes 1 is 3.

[0040] Specifically, in this embodiment, the diameters of the two connecting ports 2 at both ends of the hollow tube 1 are the same.

[0041] It also includes a cover 3, which is detachably connected to the connection port 2 to seal one end of the hollow tube 1. Since the diameter of the connection port 2 at both ends of the hollow tube 1 is the same in this embodiment, the number of cover 3 is not specifically limited. The number of cover 3 is at least 1 and at most 2 times the number of hollow tubes 1, so as to seal the two connection ports 2 corresponding to each end of each hollow tube 1.

[0042] It also includes a collection tube 4, on which a collection port 5 is formed. The collection port 5 is specifically located at one end of the collection tube 4, and the other end of the collection tube 4 is an integral closed structure.

[0043] Furthermore, both the hollow tube 1 and the collection tube 4 are made of polypropylene to reduce their weight while ensuring the chemical stability of the sample or reagent.

[0044] In another embodiment, the hollow tube 1 and the collecting tube 4 may also be made of glass, ceramic or plastic.

[0045] It also includes a filter unit 6, with two hollow tubes 1 connected to corresponding connection ports 2 at both ends of the filter unit 6, or the two ends of the filter unit 6 are detachably connected to a collection port 5 and a connection port 2, respectively. The filter unit 6 is used to effectively separate the sample into the hollow tube 1 of the next operation under preset operating conditions.

[0046] Specifically, the filter unit 6 includes a filter base 8, on which a through filter hole 9 is provided, and a filter element 10 is provided inside the filter hole 9. The pore diameter of the filter element 10 includes 0.4um, 0.2um and 0.1um. It is known that the pore diameter of the filter element 10 can also include other sizes that can achieve the same function. Both ends of the filter base 8 are provided with connecting sections 11, which are used to connect the connecting port 2 or the collecting port 5.

[0047] In this embodiment, a threaded section is formed on the outside of the connecting section 11, and the connecting port 2 or the collecting port 5 is connected by the threaded section. The use of threaded connection can ensure the sealing effect at the connection and achieve quick disassembly and assembly.

[0048] Specifically, filter element 10 is made of nylon, and filter base 8 is made of polypropylene. Polypropylene provides good chemical stability for filter base 8, making it convenient for different types of reagents to be used.

[0049] Furthermore, the threaded section is located inside the connecting section 11, so that the threaded area of ​​the connecting port 2 or the collecting port 5 is located on the outer wall, thereby avoiding the problem that the sample is easily left in the thread due to the thread, so as to ensure the full separation of the sample during centrifugation, and also to facilitate the subsequent cleaning of the hollow tube 1 and the collecting tube 4.

[0050] In another embodiment, the threaded section is located outside the connecting section 11, so that the threaded area of ​​the connecting port 2 or the collecting port 5 is located on the inner wall.

[0051] Specifically, the cover 3 includes a sealing surface and a threaded ring integrally connected to the sealing surface, wherein the inner side of the threaded ring is threadedly connected to the connection port 2.

[0052] The assembleable and reusable multifunctional QuEChERS purification tube provided in this embodiment is used as follows:

[0053] 1. Take three hollow tubes 1, and name them Hollow Tube 1 No. 1, Hollow Tube 2 No. 1 and Hollow Tube 3 No. 1 respectively. Connect the cap 3 to one end of Hollow Tube 1 No. 1. At this time, one end of Hollow Tube 1 No. 1 is closed. Add the sample into Hollow Tube 1 No. 1 and add the extraction liquid. Then perform a vortex operation on it.

[0054] 2. After the vortex is completed, connect one end of the first hollow tube 1 to the filter unit 6, and take the second hollow tube 1, connecting one end of its port 2 to the filter unit 6. At this time, the first hollow tube 1 and the second hollow tube 1 are connected in series through the filter unit 6. Further connect the cover 3 to the end of the second hollow tube 1 away from the filter unit 6. At this time, both ends of the second hollow tube 1 are closed. Further centrifuge the whole thing, so that the extract is automatically transferred to the second hollow tube 1 through the filter unit 6 and centrifugal force.

[0055] 3. Further separate the second hollow tube 1 from the filter unit 6, add salt reagent into the second hollow tube 1 and perform vortex salting-out operation.

[0056] 4. After salting out, connect the second hollow tube 1 to the filter unit 6, and connect the other end of the filter unit 6 to the third hollow tube 1. Seal the end of the third hollow tube 1 away from the filter unit 6 through the cover 3 to achieve sealing of both ends of the third hollow tube 1. Then, centrifuge the whole thing so that the salting out liquid is automatically transferred to the third hollow tube 1 through the filter unit 6 under the action of centrifugal force.

[0057] 5. Separate hollow tube 1 from filter unit 6, and add purification reagent into hollow tube 1 to further perform vortex operation.

[0058] 6. After purification, connect the third hollow tube 1 to the filter unit 6, and connect the other end of the filter unit 6 to the collection tube 4. Then, centrifuge the whole unit to allow the purified liquid to be automatically transferred to the collection tube 4 under the action of centrifugal force through the filter unit 6.

[0059] 7. Place collection tube 4 in a nitrogen blower for nitrogen blowing and concentration. After concentration, add the reconstitution solution and vortex for several minutes to reconstitute it. Then, perform centrifugation. Filter the supernatant after centrifugation through a syringe filter head into a sample vial for testing.

[0060] In the above steps, the filter element 10 corresponding to the filter unit 6 between hollow tube 1 and hollow tube 2, the filter unit 6 between hollow tube 1 and hollow tube 3, and the filter unit 6 between hollow tube 1 and collection tube 4 all have different pore sizes.

[0061] Since the supernatant and base liquid of the sample are separated by the filtration unit 6, the accuracy of the test data is guaranteed, while the use of internal standard reagents is avoided, thus greatly reducing costs.

[0062] Centrifugal filtration via filtration unit 6 allows for the use of extracts with varying viscosities, ensuring the separation of extracts with higher viscosity and solving the problem of manually filtering highly viscous extracts.

[0063] The multifunctional QuECHERS purification tube is assembleable and reusable. Its usage can also be as follows: Before vortexing, assemble the three hollow tubes 1, filter unit 6, and collection tube 4 as a single unit. Add the corresponding experimental reagents to the three hollow tubes 1 and directly vortex. After vortexing, centrifuge to allow the sample to enter the hollow tube of the next experimental stage. Repeat vortexing and centrifugation, thus requiring only one assembly operation to complete sample collection, greatly simplifying experimental steps and shortening experimental time. The filter unit 6, by setting different pore sizes in the filter element 10, allows liquid to pass through into the next hollow tube while preventing it from entering the next subsequent hollow tube under specific centrifugal forces.

[0064] Figure 3 This is the second embodiment of the present invention, which differs from the first embodiment in that:

[0065] Two hollow tubes 1 are respectively inserted into the two ends of the filter unit 6, corresponding to the connection ports 2. Specifically, the two ends of the filter unit 6 are the connecting sections 11 at both ends of the filter base 8. The connecting section 11 forms a groove in the circumference. The sealing ring 7 is fitted onto the groove and is used to seal the gap between the filter unit 6 and the connection port 2 when the filter unit 6 is inserted into the connection port 2.

[0066] The plug-in connection method enables quick assembly and disassembly of the hollow tube 1 and the filter unit 6.

[0067] Furthermore, in this embodiment, the cover 3 is also connected and sealed to the hollow tube 1 by a plug-in method.

[0068] Figure 4 This is the third embodiment of the present invention. The difference from the above embodiments is that the diameters of the connecting ports 2 at both ends of the hollow tube 1 are different, and the number of caps 3 is at least 2, with the two caps 3 respectively adapted to the two connecting ports 2.

[0069] Specifically, the diameter of the hollow tube 1 gradually decreases from one end of the connection port 2 to the other end of the connection port 2, thereby forming a trumpet-shaped structure in the hollow tube 1, which in turn achieves better centrifugation and filtration effects during the centrifugation process.

[0070] In this embodiment, the aperture of the connecting segments 11 at both ends of the filter base 8 is adapted to the aperture of the two connecting ports 2, so as to realize the connection of the two connecting ports 2 with different apertures.

[0071] Figure 5 This is the fourth embodiment of the present invention. The difference from the above embodiments is that the filter unit 6 and the hollow tube 1 are integrally connected. Specifically, the integral connection is that the filter seat 8 is fixedly disposed at one end of the hollow tube 1, preferably at the end of the connection port 2 with a smaller aperture.

[0072] Using a fixed filter unit reduces the need for disassembly and assembly, thus making it easier to use.

[0073] Therefore, in this embodiment, the two hollow tubes 1 with different apertures can be connected to each other, thereby enabling quick assembly and disassembly.

[0074] Specifically, the connection between the two hollow tubes 1 with different apertures 2 is achieved through the connecting section 11 at one end of the filter seat 8 to ensure the filtration effect between the two.

[0075] Figure 6 This is the fifth embodiment of the present invention. The difference from the above embodiments is that the filter unit 6 is embedded in one end of the hollow tube 1, and the two hollow tubes 1 are interlocked with each other. Specifically, the end of the hollow tube 1 with a smaller aperture is interlocked with the end of the other hollow tube 1 with a larger aperture. In this embodiment, the sealing ring 7 is disposed on the outside of the end of the hollow tube with a smaller aperture, and seals the gap between it and the other hollow tube after interlocking. In another embodiment, the sealing ring 7 can also be disposed on the inner wall of the end of the hollow tube 1 with a larger aperture.

[0076] Therefore, in this embodiment, the two hollow tubes 1 with different apertures can be plugged into each other, thereby enabling quick assembly and disassembly of the two.

[0077] Furthermore, in this embodiment, the hollow tube 1 and the collecting tube 4 are connected to each other, specifically, the end of the hollow tube 1 with the smaller aperture is connected to the collecting port 5.

[0078] The above description is merely a preferred embodiment of this utility model. The protection scope of this utility model is not limited to the above embodiments. All technical solutions falling within the scope of this utility model's concept are protected. It should be noted that for those skilled in the art, any improvements and modifications made without departing from the principle of this utility model should also be considered within the protection scope of this utility model.

Claims

1. A multifunctional QuEChERS purification tube that can be assembled and reused, characterized in that, include: At least two hollow tubes (1), each of which has a connection port (2) at both ends; Cover (3), which is detachably connected to the connection port (2) to seal one end of the hollow tube (1); A collection tube (4) is provided with a collection port (5); The filter unit (6) is connected to two hollow tubes (1) with corresponding connection ports (2) at both ends; or the filter unit (6) is connected to a collection port (5) and a connection port (2) at both ends.

2. The assemblable and reusable multifunctional QuEChERS purification tube according to claim 1, characterized in that, The filter unit (6) is connected to two hollow tubes (1) with corresponding connection ports (2) at both ends.

3. The assemblable and reusable multifunctional QuEChERS purification tube according to claim 2, characterized in that, The filter unit (6) is provided with sealing rings (7) at both ends. When the filter unit (6) is inserted into the connection port (2), the sealing rings (7) are used to seal the gap between the filter unit (6) and the connection port (2).

4. The assemblable and reusable multifunctional QuEChERS purification tube according to claim 1, characterized in that, The collection port (5) / connection port (2) is threadedly connected to the filter unit (6).

5. The assemblable and reusable multifunctional QuEChERS purification tube according to any one of claims 1-4, characterized in that, The filter unit (6) includes a filter seat (8), which has a through filter hole (9) and a filter element (10) inside the filter hole (9). Both ends of the filter seat (8) are provided with connecting sections (11), which are used to connect to the connecting port (2) or the collecting port (5).

6. The assemblable and reusable multifunctional QuEChERS purification tube according to claim 5, characterized in that, The filter element (10) has pore sizes of 0.4 μm, 0.2 μm and 0.1 μm.

7. The assemblable and reusable multifunctional QuEChERS purification tube according to claim 1, characterized in that, The two ends of the hollow tube (1) have different diameters of the connecting ports (2), and the number of the cover (3) is at least 2, with the two cover (3) respectively adapted to the two connecting ports (2).

8. The assemblable and reusable multifunctional QuEChERS purification tube according to claim 7, characterized in that, The diameter of the hollow tube (1) gradually decreases from one end of the connection port (2) to the other end of the connection port (2).

9. The assemblable and reusable multifunctional QuEChERS purification tube according to claim 8, characterized in that, The two hollow tubes (1) with different apertures (2) can be connected to each other.

10. The assemblable and reusable multifunctional QuEChERS purification tube according to any one of claims 7-9, characterized in that, The filter unit (6) is integrally connected to the hollow tube (1).

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