Rapid digestion filter device for respiratory secretions samples

Abstract

The utility model relates to sample detection device technical field, concretely, it relates to respiratory tract secretion sample fast digestion filter device, including the outer shock box, the inside of outer shock box is provided with the inner fender, and a plurality of springs are fixedly installed between the bottom wall of inner fender and outer shock box, the bottom surface center position department of inner fender is fixedly installed with micro shock motor, the upper surface of inner fender is fixedly installed with support frame, and a plurality of storage holes are arranged in support frame, and the storage hole is used for placing digestion treatment subassembly, and digestion treatment subassembly includes transparent cylinder, and the top cylinder of transparent cylinder is threadedly connected with sealing cover, and the bottom fixed mounting of transparent cylinder has the lead -through pipe, and the lead -through pipe is provided with manual valve, and the bottom end fixed mounting of lead -through pipe has filter cartridge, and nylon coarse filter screen and fine filter screen are fixedly installed in succession on the inner wall of filter cartridge from top to bottom. The utility model can carry out the operation of oscillation, promote the progress of mixing and digestion, and have the advantage of precision filtration simultaneously.

Description

Technical Field

[0001] This utility model relates to the field of sample detection device technology, specifically to a rapid digestion and filtration device for respiratory secretion samples. Background Technology

[0002] Respiratory secretion samples (such as sputum) contain a large amount of mucus, epithelial cells and impurities, and require pretreatment before nucleic acid extraction or pathogen culture, which is a key step in medical testing; When performing pretreatment operations, a corresponding digestion and filtration device is usually used. The digestion and filtration device can perform reasonable pretreatment operations on the sample. When performing processing operations, the existing digestion and filtration device uses manual addition of digestive enzyme followed by standing for 10-15 minutes. However, this direct addition process will cause poor fluidity of digestive enzyme and original solution, and the shaking operation cannot be performed. This will result in incomplete decomposition of mucus, poor sample homogeneity, and affect the subsequent extraction efficiency. Furthermore, traditional digestion and filtration devices also suffer from incomplete impurity removal. For example, traditional digestion and filtration devices use ordinary filter membranes, which have relatively large pore sizes. These membranes can only intercept large particles, leaving small cell debris and fibers behind, interfering with nucleic acid amplification or culture results and leading to false negatives or false positives. Therefore, we propose a rapid digestion and filtration device for respiratory secretion samples. Utility Model Content

[0003] The purpose of this invention is to provide a rapid digestion and filtration device for respiratory secretion samples to address the deficiencies mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution: A rapid digestion and filtration device for respiratory secretions includes an external shaking chamber with an inner pad inside. Multiple springs are fixedly installed between the inner pad and the bottom wall of the external shaking chamber. A miniature oscillation motor is fixedly installed at the center of the bottom surface of the inner pad. A support frame is fixedly installed on the upper surface of the inner pad, and multiple storage holes are provided within the support frame for placing a digestion processing component. The digestion processing component includes a transparent cylinder with a threaded sealing cap at the top. A guide tube is fixedly installed at the bottom of the transparent cylinder, and a manual valve is installed on the guide tube. A filter cylinder is fixedly installed at the bottom end of the guide tube, and a nylon coarse filter and a fine filter are sequentially fixedly installed on the inner wall of the filter cylinder from top to bottom.

[0005] Preferably, the bottom center of the external vibration chamber is connected to the outside, and a bottom inspection cover is detachably installed on the part of the bottom of the external vibration chamber that is connected to the outside. This feature facilitates subsequent disassembly and maintenance of the micro-vibration motor.

[0006] Preferably, the size of the inner pad is smaller than the size of the outer vibration chamber, and a flexible side sealing pad is fixedly installed between the side of the inner pad and the inner wall of the outer vibration chamber. This feature effectively seals the space between the inner pad and the inner wall of the outer vibration chamber, preventing impurities from falling to the bottom of the outer vibration chamber. At the same time, the flexible side sealing pads of the flexible structure will not affect the normal vibration of the inner pad.

[0007] Preferably, the digestion processing component is inserted into the storage hole, and rubber protective pads are provided on the bottom wall and side wall of the storage hole. This feature uses a rubber protective pad to protect the digestion and processing components, preventing them from colliding directly with the walls of the storage holes and causing damage.

[0008] Preferably, both the transparent cylinder and the filter cylinder have a transparent cylindrical structure, and the bottom of both the transparent cylinder and the filter cylinder are conical.

[0009] Preferably, a rubber stopper is fixedly installed at the center of the sealing cap, and the bottom end of the rubber stopper is inserted into the transparent cylinder.

[0010] Preferably, both the coarse nylon filter screen and the fine filter screen have serrated cross-sections, and a centrifuge tube docking tube is fixedly installed at the bottom end of the filter cylinder; This setting increases the filtration area of ​​the nylon coarse and fine filters.

[0011] Preferably, the height of the storage hole is greater than the height of the filter cylinder, and the thickness of the rubber protective pad is between 0.3cm and 0.6cm; This design provides the rubber protective pad with good cushioning protection, and also prevents the digestion and processing components from easily detaching from the storage hole due to vibration. Compared with the prior art, the beneficial effects of this utility model are: 1. This utility model incorporates a miniature oscillating motor, spring, and inner pad inside an external shaking chamber, along with a support frame for placing the digestion processing components. After the digestive enzyme is added, the miniature oscillating motor can be activated to drive the inner pad and digestion processing components to vibrate slightly and synchronously. This achieves thorough mixing of the digestive enzyme with the respiratory secretion sample, thereby accelerating mucus decomposition, improving sample uniformity, shortening digestion time, and ensuring the efficiency of subsequent nucleic acid extraction.

[0012] 2. This utility model arranges a nylon coarse filter and a fine filter in the filter tube of the digestion processing component from top to bottom, and both have a serrated cross-section. The nylon coarse filter first intercepts large particulate impurities, and then the fine filter filters out small cell debris and fibers. At the same time, the serrated structure increases the filtration area, so as to achieve graded and efficient removal of impurities in the sample, thereby reducing interference in the subsequent nucleic acid amplification or culture process and reducing the probability of false negative or false positive results.

[0013] 3. This utility model designs the digestion and processing component as a transparent structure combining a transparent cylinder and a filter cylinder, both with conical bottoms. Combined with the rubber stopper on the sealing cap and the manual valve on the guide tube, it facilitates intuitive observation of the entire process of sample digestion and filtration. The rubber stopper ensures a sealing effect, and the manual valve controls the liquid flow rate. At the same time, the conical bottom facilitates the concentrated flow of the sample, thus achieving visualization and controllability of the device operation. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This utility model Figure 1 Enlarged view of point A in the middle; Figure 3 This is a schematic diagram of the digestion processing component of this utility model; The meanings of the labels in the diagram are as follows: 1. External vibration chamber; 10. Inner pad; 11. Flexible side sealing pad; 12. Spring; 13. Miniature vibration motor; 14. Bottom inspection cover; 15. Support frame; 16. Storage hole; 17. Rubber protective pad; 2. Digestion processing components; 20. Transparent cylinder; 21. Sealing cap; 211. Rubber stopper; 22. Conductor tube; 221. Manual valve; 23. Filter cylinder; 24. Nylon coarse filter screen; 25. Fine filter screen; 26. Centrifuge tube docking tube. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Please see Figures 1-3 This utility model provides a technical solution: a rapid digestion and filtration device for respiratory secretion samples, including an outer shaking chamber 1, an inner pad 10 inside the outer shaking chamber 1, multiple springs 12 fixedly installed between the inner pad 10 and the bottom wall of the outer shaking chamber 1, a micro-vibration motor 13 fixedly installed at the center of the bottom surface of the inner pad 10, and a support frame 15 fixedly installed on the upper surface of the inner pad 10. The support frame 15 has multiple storage holes 16, which are used to place digestion processing components 2. The micro-vibration motor 13 can drive the inner pad 10 and the support frame 15 to vibrate, and the springs 12 can help maintain stable vibration, so that the digestion processing components 2 in the storage holes 16 vibrate synchronously, allowing the digestive enzymes to be fully mixed with the sample, solving the problems of uneven mixing and incomplete mucus decomposition after manual dripping.

[0017] In this embodiment, the digestion treatment component 2 includes a transparent cylinder 20, which contains pre-stored digestive enzymes. A sealing cap 21 is threaded onto the top of the transparent cylinder 20 to ensure a sealing effect. A guide tube 22 is fixedly installed at the bottom of the transparent cylinder 20, and a manual valve 221 is provided on the guide tube 22. A filter cylinder 23 is fixedly installed at the bottom end of the guide tube 22. A nylon coarse filter screen 24 and a fine filter screen 25 are fixedly installed on the inner wall of the filter cylinder 23 from top to bottom, so that the nylon coarse filter screen 24 and the fine filter screen 25 can be used to filter the treatment liquid during use.

[0018] Specifically, the bottom center of the external vibration box 1 is connected to the outside. The bottom of the external vibration box 1 is detachably equipped with a bottom inspection cover 14. The detachable installation method allows the operator to easily open the connecting part at the bottom of the external vibration box 1 to inspect or maintain the internal micro vibration motor 13, thus improving the maintenance convenience of the device.

[0019] Furthermore, the inner pad 10 is smaller than the outer vibration chamber 1. A flexible side sealing pad 11 is fixedly installed between the side of the inner pad 10 and the inner wall of the outer vibration chamber 1. The flexible structure not only seals the gap between the two to prevent impurities from falling to the bottom of the outer vibration chamber 1 and causing pollution, but also does not hinder the normal vibration of the inner pad 10, thus taking into account both sealing performance and vibration stability.

[0020] like Figure 1 and Figure 2 As shown, the digestion processing component 2 is inserted into the storage hole 16. Rubber protective pads 17 are provided on the bottom wall and side wall of the storage hole 16. The rubber protective pads 17 can isolate the digestion processing component 2 from direct contact with the hole wall of the storage hole 16, and prevent the two from colliding during vibration, thus avoiding damage to the digestion processing component 2. They play a good buffering and protection role.

[0021] like Figure 3 As shown, both the transparent tube 20 and the filter tube 23 are transparent cylindrical structures. The bottom of both the transparent tube 20 and the filter tube 23 are conical. The transparent structure makes it easy for operators to observe the digestion and filtration status of the sample in real time, while the conical bottom facilitates the concentrated flow of the sample in the direction of the guide tube 22, improving the intuitiveness of the operation and the smoothness of the sample flow.

[0022] like Figure 3 As shown, a rubber stopper 211 is fixedly installed at the center of the sealing cap 21. The bottom end of the rubber stopper 211 is inserted into the transparent tube 20. The rubber stopper 211 allows the needle at the front end of the syringe to be inserted for injection of the original liquid without unscrewing the sealing cap 21.

[0023] like Figure 3 As shown, both the coarse nylon filter 24 and the fine nylon filter 25 have serrated cross-sections. A centrifuge tube docking tube 26 is fixedly installed at the bottom of the filter cylinder 23. The serrated structure increases the filtration area of ​​both. The coarse nylon filter 24 can first intercept large particulate impurities, and the fine nylon filter 25 can then filter out fine cell debris and fibers, achieving graded filtration and improving the thoroughness of impurity removal. The centrifuge tube docking tube 26 at the bottom of the filter cylinder 23 facilitates direct docking with centrifuge tubes, enabling rapid collection of filtered samples. The pore size of the coarse nylon filter 24 is between 80 and 120 μm, intercepting large particulate impurities. The fine nylon filter 25 is a mixed cellulose ester filter membrane with a pore size between 0.5 and 1.2 μm, removing fine cell debris and fibers, making the filtration operation more thorough.

[0024] It is worth noting that the height of the storage hole 16 is greater than the height of the filter cylinder 23, and the thickness of the rubber protective pad 17 is between 0.3cm and 0.6cm. The appropriate thickness gives the rubber protective pad 17 good buffering performance. The higher storage hole 16 can effectively limit the digestion and treatment component 2, preventing it from falling out of the storage hole 16 during vibration, thus improving the stability of the device operation.

[0025] Finally, it should be noted that the micro oscillation motor 13, the corresponding control system, and the external power supply of this utility model are all general standard parts or parts known to those skilled in the art. Their structure and principle can be known to those skilled in the art through technical manuals or conventional experimental methods. In the idle space of this device, all the above-mentioned electrical components, which refer to power elements, electrical components, and the matching controller and power supply, are connected by wires. The specific connection method should refer to the working principle of this utility model. The electrical connections between each electrical component are completed in the order of operation. The detailed connection methods are all technologies known in the art.

[0026] When using the rapid digestion and filtration device for respiratory secretion samples of this utility model, insert the syringe needle into the rubber stopper 211 in the center of the sealing cap 21 to inject the respiratory secretion sample into the transparent tube 20, or unscrew the sealing cap 21 and transfer the sample into the transparent tube 20 using external tools. Insert the transparent tube 20 into the storage hole 16 of the support frame 15. The rubber protective pad 17 in the storage hole 16 can prevent the components from being damaged by collision. Then the micro oscillation motor 13 is started, which drives the inner pad plate 10 and the support frame 15 to vibrate. The spring 12 helps to maintain stable vibration. The side flexible sealing pad 11 seals the gap without affecting the vibration, so that the digestive enzyme in the transparent tube 20 is fully mixed and decomposed with the sample. During this period, the digestion status can be observed through the transparent tube 20. After digestion is complete, remove the digestion processing component 2, align the connecting tube 26 with the external centrifuge tube, open the manual valve 221 on the connecting tube 22, and the sample flows into the filter cartridge 23 through the conical bottom connecting tube 22. First, it passes through the nylon coarse filter screen 24 to intercept large particles of impurities, then through the fine filter screen 25 to filter out small fragments, and finally connects to the centrifuge tube through the connecting tube 26. The filtered sample is collected for subsequent testing.

[0027] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A rapid digestion and filtration device for respiratory secretions, comprising an external shaking chamber (1), characterized in that: The outer vibration chamber (1) is provided with an inner pad (10). Multiple springs (12) are fixedly installed between the inner pad (10) and the bottom wall of the outer vibration chamber (1). A miniature vibration motor (13) is fixedly installed at the center of the bottom surface of the inner pad (10). A support frame (15) is fixedly installed on the upper surface of the inner pad (10). Multiple storage holes (16) are provided inside the support frame (15). The storage holes (16) are used to place digestion processing components. 2) The digestion processing component (2) includes a transparent cylinder (20), a sealing cap (21) is threaded onto the top of the transparent cylinder (20), a guide tube (22) is fixedly installed at the bottom of the transparent cylinder (20), a manual valve (221) is provided on the guide tube (22), a filter cylinder (23) is fixedly installed at the bottom end of the guide tube (22), and a nylon coarse filter screen (24) and a fine filter screen (25) are fixedly installed on the inner wall of the filter cylinder (23) from top to bottom.

2. The rapid digestion and filtration device for respiratory secretions according to claim 1, characterized in that: The bottom center of the external vibration box (1) is connected to the outside world, and a bottom inspection cover (14) can be detachably installed on the bottom part of the external vibration box (1) that is connected to the outside world.

3. The rapid digestion and filtration device for respiratory secretions according to claim 1, characterized in that: The inner pad (10) is smaller than the outer vibration box (1), and a flexible side sealing pad (11) is fixedly installed between the side of the inner pad (10) and the inner wall of the outer vibration box (1).

4. The rapid digestion and filtration device for respiratory secretions according to claim 1, characterized in that: The digestion processing component (2) is inserted into the storage hole (16), and rubber protective pads (17) are provided on the bottom wall and side wall of the storage hole (16).

5. The rapid digestion and filtration device for respiratory secretions according to claim 1, characterized in that: Both the transparent cylinder (20) and the filter cylinder (23) are transparent cylindrical structures, and the bottom of the transparent cylinder (20) and the bottom of the filter cylinder (23) are conical.

6. The rapid digestion and filtration device for respiratory secretions according to claim 1, characterized in that: A rubber plug (211) is fixedly installed at the center of the sealing cap (21), and the bottom end of the rubber plug (211) is inserted into the transparent tube (20).

7. The rapid digestion and filtration device for respiratory secretions according to claim 1, characterized in that: The cross-sections of the nylon coarse filter (24) and the fine filter (25) are both serrated, and a centrifuge tube docking tube (26) is fixedly installed at the bottom of the filter cylinder (23).

8. The rapid digestion and filtration device for respiratory secretions according to claim 4, characterized in that: The height of the storage hole (16) is greater than the height of the filter cylinder (23), and the thickness of the rubber protective pad (17) is between 0.3cm and 0.6cm.

Images