Pathology slide staining wet box

Abstract

This utility model discloses a wet box for staining pathological slides, including a box body; the interior of the box body is provided with several partitions, which divide the interior of the box body into several placement areas; a slide placement plate is rotatably arranged in each placement area; several sets of first protrusions are provided laterally on the upper end of each slide placement plate; a second protrusion is provided longitudinally on the upper end of each slide placement plate; a cover film is provided on the upper end of each slide placement plate; several partition cavities are formed between the cover film, the slide placement plate, the first protrusions, and the second protrusions; a reagent dispensing part extends from the other end of the cover film; the box body is provided with an inlet pipe and an outlet pipe; the inlet pipe and the distribution pipe are connected; an outlet is opened on the distribution pipe corresponding to the position above each partition cavity; the outlet pipe is connected to the bottom of each of the several placement areas. The wet box for staining pathological slides provided by this utility model avoids frequent handling of slides during operation, simplifies the operation steps, saves time, and also saves reagents.

Description

Technical Field

[0001] This utility model relates to the field of humidification chamber technology, and in particular to a humidification chamber for staining pathological sections. Background Technology

[0002] Pathology is a fundamental medical discipline that studies the causes and mechanisms of human diseases. In clinical practice, pathology is generally considered the gold standard for disease diagnosis. The material basis for pathologists to diagnose diseases is pathological slides.

[0003] Generally, the sectioned tissue is attached to a glass slide, followed by various types of pathological staining. Pathological staining types are diverse, including but not limited to HE staining, Masson staining, Oil Red O staining, etc. In practice, the staining process for each type often varies considerably. Some staining requires light-proof and humidified conditions; in some cases, due to limitations, manual staining is necessary. Both types of staining generally require a light-proof box that can hold the slide and can hold a small amount of water; this box is called a humidified box.

[0004] Generally, pathological slides are of a fixed size, 76*25.5mm. The process of using a humidified chamber is roughly as follows: Dewax the slides to water, use a water-resistant pen to draw circles around the tissue (this is to prevent the staining reagent from spreading outside the tissue), place the slides flat in the humidified chamber, and wait for the staining time to complete. Remove the slides from the humidified chamber, rinse them with water or perform other steps, and then place them back into the chamber. This process may be repeated several times depending on the staining method. Finally, after all staining steps are completed, remove the slides and mount them.

[0005] However, existing humidified chambers are cumbersome to operate, requiring multiple slide removals and placements during staining, increasing the operational difficulty. They also require the use of a water-blocking pen, as the staining process necessitates multiple cleanings and often multiple circular strokes. The water-blocking effect of the pen diminishes significantly after several reagent additions or rinses, leading to reagent overflow. For larger tissues that cover most of the slide, the pen may fail to reach the tissue edges or even damage the tissue, causing reagent to easily spread beyond the slide and resulting in waste. Furthermore, due to varying operator techniques, the thickness of the droplets after drawing the circles may differ, potentially consuming even more reagent. Utility Model Content

[0006] In view of this, the present invention proposes a wet chamber for staining pathological sections.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: A wet box for staining pathological sections includes a box body; the box body has several partitions inside, which divide the interior of the box body into several placement areas; characterized in that a slide placement plate is rotatably provided in each placement area; each slide placement plate has several sets of first protrusions laterally provided at its upper end, which divide the slide placement plate into several slide placement areas; each slide placement plate has a second protrusion longitudinally provided at its upper end corresponding to one side of each slide placement area; each slide placement plate has a cover film provided at its upper end through a third protrusion; the cover film, the slide placement plate, the first protrusions and the second protrusions together form several partition cavities; a reagent dispensing section extends from the other end of the cover film; The box is equipped with an inlet pipe and an outlet pipe; the inlet pipe is connected to the distribution pipe; the distribution pipe has an outlet corresponding to the position above each partition cavity; the outlet pipe is connected to the bottom of several placement areas.

[0008] Furthermore, each group of first protrusions includes two first protrusions; there is a gap between the two first protrusions.

[0009] Furthermore, the bottom end of the cover film is provided with several third protrusions; the several third protrusions are respectively embedded in several of the gaps.

[0010] Furthermore, the top surface of the reagent dispensing section is an inclined surface, which is inclined downward along the direction away from the cover film.

[0011] Furthermore, the bottom surface of the cover film is lower than the top surface of the second protrusion.

[0012] Furthermore, the upper end of the glass slide placement plate is provided with several limiting grooves.

[0013] Furthermore, several of the partitions are provided with receiving grooves; the water distribution pipe is embedded in the receiving grooves; The top height of the water distribution pipe is not higher than the top height of the box body.

[0014] Furthermore, a wet box cover is provided on the top of the box body.

[0015] Furthermore, a valve is provided on the water inlet pipe.

[0016] Compared with existing technologies, the beneficial effects of this utility model are: (1) The wet box for staining pathological sections provided by this utility model has a water channel, which simplifies the staining operation, avoids frequent picking and putting of slides during the operation, saves time, and simplifies the process. (2) The wet box for staining pathological sections provided by this utility model is designed with a cover film, so that the cover film system replaces the water-blocking pen to draw circles. The capillary effect is used to make the added reagent evenly diffused onto the glass slide. The cover film system controls the thickness of the droplets generated by the added reagent to 0.8-1.2 mm, and the total amount of reagent used is about 50-80 microliters. Under the same conditions, if the wet box is not used and the water-blocking pen is used, the amount of reagent used is about 100 microliters, and it is more difficult to draw circles with the water-blocking pen. Attached Figure Description

[0017] Figure 1 A perspective view of a wet chamber for staining pathological sections provided in an embodiment of this utility model; Figure 2 A perspective view of a wet box for staining pathological sections without the wet box cover provided in an embodiment of this utility model; Figure 3 A top view of a wet chamber for staining pathological sections without the wet chamber lid provided in an embodiment of this utility model; Figure 4 for Figure 3 Sectional view along line AA in the middle; Figure 5 A perspective view of the glass slide placement assembly provided in an embodiment of this utility model; Figure 6 A top view of the slide placement assembly provided in an embodiment of this utility model; Figure 7 A perspective view of a wet chamber for staining pathological sections after placing a glass slide, provided in an embodiment of this utility model; Figure 8 A top view of a wet chamber for staining pathological sections after placing a glass slide, provided in an embodiment of this utility model; Figure 9 for Figure 8 BB-direction sectional view in the middle; Figure 10 A perspective view of the glass slide placement assembly provided in an embodiment of this utility model; Figure 11 for Figure 10 Another structural diagram; Figure 12 A top view of the glass slide assembly provided in an embodiment of this utility model; Figure 13 Right view of the glass slide assembly provided in an embodiment of this utility model; Figure 14 An exploded view of the glass slide assembly provided in an embodiment of this utility model.

[0018] In the diagram: 1. Box body; 2. Wet box lid; 3. Divider; 4. Placement area; 5. Slide placement plate; 6. Rotating shaft; 7. Gap; 8. Limiting groove; 9. Slide; 10. First protrusion; 11. Second protrusion; 12. Cover film; 13. Third protrusion; 14. Cavity; 15. Reagent dispensing section; 16. Inlet pipe; 17. Outlet pipe; 18. Divider pipe; 19. Outlet; 20. Separating cavity; 21. Receptacle. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0020] In the description of this utility model, unless otherwise stated, "a plurality of" means two or more; the terms "upper," "lower," "left," "right," "inner," "outer," "front end," "rear end," "head," "tail," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. In addition, the terms "first," "second," "third," etc., are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0021] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "connected" and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0022] Example: like Figure 1-14 As shown, the wet chamber for staining pathological sections includes a box body 1; a wet chamber lid 2 is provided on top of the box body 1. Both the box body 1 and the wet chamber lid 2 are black, forming a light-proof box.

[0023] The interior of the box 1 is provided with several partitions 3; the partitions 3 divide the interior of the box 1 into several placement areas 4. Each placement area 4 is provided with a glass slide placement plate 5.

[0024] A rotating shaft 6 is inserted inside the slide placement plate 5, and the slide placement plate 5 and the rotating shaft 6 are fixedly connected. One end of the rotating shaft 6 extends out of the box body 1.

[0025] Each slide placement plate 5 has several sets of first protrusions 10 arranged horizontally at its upper end, which divide the slide placement plate 5 into several slide placement areas. Each set of first protrusions 10 includes two first protrusions 10, with a gap 7 between the two first protrusions 10. Each slide placement plate 5 has several second protrusions 11 arranged vertically at its upper end, with the second protrusions 11 located on the left side of each of the several slide placement areas.

[0026] The bottom end of the cover film 12 is provided with several third protrusions 13; these third protrusions 13 are respectively embedded in several gaps 7 (several sets of first protrusions 10 form several gaps 7). The cover film 12, the slide placement plate 5, the first protrusions 10, and the second protrusions 11 together form several partitioned cavities 20. The height of the third protrusions 13 is higher than the height of the first protrusions 10. The bottom surface of the cover film 12 is lower than the top surface of the second protrusions 11.

[0027] A reagent dispensing section 15 extends from the right end of the cover film 12. After glass slides are placed one by one in the partition cavity 20, the reagent dispensing section 15 is located above the right side of the glass slide 9.

[0028] The function of the second protrusion 11 is to prevent the glass slide 9 from falling off when the rotating shaft 6 rotates.

[0029] The reagent dispensing section 15 has a triangular cross-section, and its top surface is an inclined surface, which is set downwards along the direction away from the cover film 12. The inclined surface facilitates the dispensing of reagent into the glass slide 9, and the reagent can be evenly diffused throughout the entire glass slide 9 under the action of capillary action.

[0030] The cover film 12, the third protrusion 13 and the reagent dispensing part 15 are all made of high-transparency PPSU.

[0031] The slide placement assembly consists of a placement area 4, a slide placement plate 5, a rotating shaft 6, a first protrusion 10, a second protrusion 11, a cover film 12, a third protrusion 13, a reagent dispensing section 15, and a limiting groove 8. A slide 9 is placed on the slide placement assembly to form a slide assembly.

[0032] The housing 1 is equipped with an inlet pipe 16 and an outlet pipe 17; one end of the inlet pipe 16 is connected to a water supply device via a hose, and the other end of the inlet pipe 16 is connected to a distribution pipe 18; the distribution pipe 18 extends into the interior of the housing 1. A valve may be installed on the inlet pipe 16.

[0033] The water distribution pipe 18 is divided into a horizontal water supply pipe and a vertical water distribution pipe 18. Several partition plates 3 are each provided with a receiving groove 21; the horizontal water distribution pipe 18 is embedded within the receiving groove 21. Each placement area 4 has a vertical water distribution pipe 18 at its upper right end, and the vertical water distribution pipes 18 are all connected to the horizontal water distribution pipes 18. Within each placement area 4, the vertical water distribution pipe 18 has an outlet 19 corresponding to the position above each partition cavity 20. If a glass slide 9 is placed in the partition cavity 20 of the slide placement plate 5, then the vertical water distribution pipe 18 also has an outlet 19 corresponding to the position above each glass slide 9, with the outlet 19 above each glass slide 9 located on the upper right side of the glass slide 9. The top height of the water distribution pipe 18 is not higher than the top height of the box body 1.

[0034] The bottom of the water outlet pipe 17 is connected to the bottom of several placement areas 4. A liquid recovery device is placed at the lower end of the water outlet pipe 17.

[0035] The slide placement plate 5 has several limiting grooves 8 on its upper end; the limiting grooves 8 extend from the other side of several slides 9 respectively. The limiting grooves 8 are designed to facilitate the placement and removal of slides 9.

[0036] Working principle: The sectioned tissue was attached to glass slide 9, and the pathological sections were dewaxed to water. Remove the humidification chamber and place the glass slides 9 one by one into the several partitioned cavities 20 on the glass slide placement plate 5 inside the humidification chamber, with the label area facing right. There is a cavity 14 between the cover film 12 and the several glass slides 9, and the height of the cavity 14 is 0.8 to 1.2 mm. Connect the water inlet pipe 16 of the wet box to the water supply equipment through a hose, with the valve on the water inlet pipe 16 in the closed position; Reagent is added to each reagent addition section 15, and the reagent enters the glass slide 9. The reagent is evenly diffused to the entire glass slide 9 by using capillary action. After the reaction time between the reagent and the pathological tissue is over, the cover film 12 is removed, the rotating shaft 6 is rotated, and the glass slide placement plate 5 is tilted to the left at a certain angle. The reacted reagent flows into the bottom of the humidifier and is discharged through the water outlet pipe 17. Open the valve on the inlet pipe 16. At this time, clean water flows into the wet box through the inlet pipe 16, the branch pipe 18, and the outlet 19 to rinse each glass slide 9. After rinsing, close the valve on the inlet pipe 16, rotate the shaft 6 again to reset the slide placement plate 5; replace the cover film 12 and perform the next reagent addition operation. Repeat the above operation after the reagent reaction time is over until the final staining of the pathological tissue is completed.

[0037] The above are merely preferred embodiments of this utility model, but the scope of protection of this utility model is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in this utility model, based on the technical solution and inventive concept of this utility model, should be included within the scope of protection of this utility model.

Claims

1. A wet box for staining pathological sections, comprising a box body (1); the box body (1) is provided with a plurality of partitions (3) inside, the partitions (3) dividing the interior of the box body (1) into a plurality of placement areas (4); characterized in that, Each placement area (4) is provided with a glass slide placement plate (5) that rotates within it; each glass slide placement plate (5) has several sets of first protrusions (10) arranged laterally at its upper end, which divide the glass slide placement plate (5) into several glass slide placement areas; each glass slide placement plate (5) has a second protrusion (11) arranged longitudinally at its upper end corresponding to one side of each glass slide placement area; each glass slide placement plate (5) has a cover film (12) arranged at its upper end through a third protrusion (13); the cover film (12), the glass slide placement plate (5), the first protrusions (10) and the second protrusions (11) together form several partition cavities (20); the other side of the cover film (12) extends to provide a reagent dispensing part (15). The box (1) is provided with an inlet pipe (16) and an outlet pipe (17); the inlet pipe (16) and the distribution pipe (18) are connected; the distribution pipe (18) is provided with an outlet (19) at the position above each partition cavity (20); the outlet pipe (17) and the bottom of several placement areas (4) are all connected.

2. The wet chamber for staining pathological sections according to claim 1, characterized in that, Each group of first protrusions (10) includes two first protrusions (10); there is a gap (7) between the two first protrusions (10).

3. A humidified chamber for staining pathological sections according to claim 2, characterized in that, The bottom end of the cover film (12) is provided with several third protrusions (13); the several third protrusions (13) are respectively embedded in several gaps (7).

4. A humidified chamber for staining pathological sections according to claim 3, characterized in that, The top surface of the reagent dispensing section (15) is an inclined surface, which is inclined downward along the direction away from the cover film (12).

5. A humidified chamber for staining pathological sections according to claim 3, characterized in that, The bottom surface of the cover (12) is lower than the top surface of the second protrusion (11).

6. A humidified chamber for staining pathological sections according to claim 1, characterized in that, Several limiting grooves (8) are provided on the other side of the glass slide placement plate (5).

7. A wet chamber for staining pathological sections according to claim 1, characterized in that, Several partition plates (3) are provided with receiving grooves (22); the water distribution pipe (18) is embedded in the receiving groove (22); The top height of the water distribution pipe (18) is not higher than the top height of the box body (1).

8. A humidified chamber for staining pathological sections according to claim 1, characterized in that, The box body (1) is provided with a wet box cover (2) on top.

9. A humidified chamber for staining pathological sections according to claim 1, characterized in that, A valve is provided on the water inlet pipe (16).

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