A mouse social behavior experiment device

Abstract

The utility model embodiment provides a mouse social behavior experiment device, including box, two laser signal collectors and two signal output ware, the box top is open, the box is isolated by two parallel first baffle in length direction and is arranged in order first compartment, second compartment and third compartment;The first baffle bottom is equipped with the opening;Two laser signal collectors emit laser level parallel to the first baffle and be located in second compartment, respectively close to first compartment and third compartment;Two signal output ware are electrically connected with different laser signal collector respectively, so as to not rely on artificial realization to the accurate synchronous marking of experimental mouse social behavior and calcium signal under the condition that not using camera and specific behavior software.

Description

Technical Field

[0001] This utility model relates to the field of animal behavior experiment technology, and in particular to an experimental device for social behavior of mice. Background Technology

[0002] Social recognition is fundamental to many social behaviors, essential for maintaining social order and promoting social development. It is a crucial behavior for social animals like humans and is therefore highly conserved in evolution. Impairment in social recognition can lead to social disorders. It is present in many mental illnesses, such as schizophrenia and autism. Schizophrenia has the highest prevalence among mental illnesses, while autism is a common neurodevelopmental disorder in children. Both can significantly reduce social recognition abilities, affecting patients' ability to integrate into society. Therefore, research on the neural mechanisms of social recognition and related diseases plays a vital role in understanding the workings of human social behavior, revealing the pathogenesis of social disorders, and improving national health. Current research on the circuit mechanisms of social recognition and its impairment in mice requires observing the activity of neuronal clusters in target brain regions while observing social recognition behavior. This often involves using fiber optic recording of calcium signals, and labeling social behavior and calcium signals to analyze the correlation between changes in these two factors.

[0003] Currently, the detection of social recognition behavior mainly utilizes the traditional three-box experimental setup. The box is divided into three areas, with different experimental mice placed on opposite sides of the box to observe their activity. Calcium signal labeling is typically done manually or using specific behavioral software.

[0004] The manual labeling method involves manually dividing the three-box experimental setup into zones, such as social and non-social areas. Then, a professional high-speed camera is used to record the mice's social behavior. After recording, calcium signals are assigned based on the time the mice enter a specific area. However, this manual labeling method requires sophisticated equipment—a professional high-speed camera—and is also time-consuming and labor-intensive, requiring frame-by-frame analysis of the behavioral videos. Alternatively, manual labeling can be performed during the experiment, but this method has very low accuracy and demands high levels of concentration and responsiveness from the experimenter.

[0005] The method of marking using specific behavior software is as follows: a camera is set up to capture images of various areas of the three-box experimental setup. The specific behavior software can divide the three-box experimental setup into social areas, non-social areas, etc., based on the images of the three-box experimental setup displayed on it. When the experimental mouse enters a specific area, a signal is output. The output signal reaches the calcium signal recording device to mark the calcium signal, so as to realize the synchronous marking of the time when the experimental mouse enters the specific area and the corresponding time of the calcium signal.

[0006] In other words, current technologies suffer from high labor costs and low accuracy when using manual labeling during experiments. Using professional high-speed cameras to record video and then manually labeling it during playback is also costly and inefficient. Improving labeling accuracy and achieving synchronized labeling requires configuring cameras and specific behavioral software, which also presents significant cost challenges; otherwise, synchronized labeling of social behaviors and calcium signals cannot be achieved. Utility Model Content

[0007] The purpose of this invention is to provide a mouse social behavior experimental device that can accurately and synchronously label the social behavior and calcium signals of experimental mice without the use of a camera and specific behavioral software, and without relying on manual intervention. The specific technical solution is as follows:

[0008] The purpose of this utility model embodiment is to provide a mouse social behavior experimental device, comprising: a box body with an open top, the box body being divided into a first compartment, a second compartment, and a third compartment in sequence by two parallel first partitions along its length; the first partitions having openings at their bottoms; two laser signal collectors, the lasers emitted by the two laser signal collectors being horizontally parallel to the first partitions and located in the second compartment, respectively close to the first compartment and the third compartment; and two signal output devices, the two signal output devices being electrically connected to different laser signal collectors.

[0009] In some embodiments of this application, it also includes:

[0010] A preparation compartment is located outside the box body and is connected to the second compartment through a first opening located on the side wall of the box body. The first opening is equidistant from the two first partitions.

[0011] The second partition is slidably disposed in the first opening.

[0012] In some embodiments of this application, each laser signal acquisition device includes: a laser transmitter and a laser receiver, which are electrically connected to the same signal output device respectively;

[0013] The laser emitter and the laser receiver are respectively located on the outside of two opposite side walls of the housing. The two opposite side walls are respectively provided with corresponding second openings. The laser emitted by the laser emitter is emitted to the laser receiver through the second openings.

[0014] In some embodiments of this application, the first partition is a transparent partition.

[0015] In some embodiments of this application, the number of openings on the first partition is multiple;

[0016] The plurality of openings are spaced apart in the width direction of the housing;

[0017] For each of the aforementioned openings, the dimension W1 in the width direction of the box body is in the range of 0.35cm≤W1≤0.45cm, and the dimension H1 in the height direction of the box body is in the range of 4.7cm≤H1≤4.9cm.

[0018] In some embodiments of this application, the height H2 of the box body is in the range of: 18cm≤H2≤22cm;

[0019] The first compartment and the third compartment have dimensions L1 ranging from 5.8cm to 6.2cm in the length direction of the box body, and dimensions W2 ranging from 6.8cm to 7.2cm in the width direction of the box body.

[0020] The second compartment has a length L2 range of 35cm≤L2≤39cm and a width W3 range of 6.8cm≤W3≤7.2cm.

[0021] In some embodiments of this application, the preparation compartment has a length L3 range of 4.8cm ≤ L3 ≤ 5.2cm and a width W4 range of 4.4cm ≤ W4 ≤ 4.6cm.

[0022] In some embodiments of this application, the first opening is provided with grooves on both sides in the height direction of the box body, the openings of the two grooves are arranged opposite to each other, and the second partition is located in the two grooves.

[0023] In some embodiments of this application, the signal output device is a relay.

[0024] In some embodiments of this application, the bottom plate and side walls of the housing are detachably connected.

[0025] Beneficial effects:

[0026] The mouse social behavior experimental device provided in this embodiment of the utility model places an experimental mouse whose calcium signal is collected by a calcium signal recording device in a second compartment, and other tool mice in a first compartment and / or a third compartment. When the experimental mouse passes by the laser signal collector near the first or third compartment and blocks the laser emitted by the laser signal collector, the laser signal collector emits a signal indicating that the experimental mouse has moved to that compartment, thus indicating that the experimental mouse has produced a social behavior of tending to or resisting social interaction with the tool mice. The laser signal collector transmits the signal to a signal output device electrically connected to the laser signal collector. The signal output device can output the received signal to the calcium signal recording device, which records the calcium signal at this time. This allows for synchronous labeling of the experimental mouse's social behavior, as indicated by the signal collected by the laser signal collector, and the calcium signal of the experimental mouse at the time of the social behavior. This process does not require manual labeling, nor does it require the configuration of a camera or specific behavioral software, thus achieving accurate synchronous labeling of the experimental mouse's social behavior and calcium signal.

[0027] Of course, any product implementing this utility model does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0028] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0029] Figure 1 A first-angle structural schematic diagram of the mouse social behavior experimental device provided in an embodiment of this application;

[0030] Figure 2 A second-angle structural schematic diagram of the mouse social behavior experimental device provided in the embodiments of this application;

[0031] Figure 3 This is a third-angle structural diagram of the mouse social behavior experimental device provided in the embodiments of this application;

[0032] Figure 4 A schematic diagram of the structure of the first partition of the mouse social behavior experimental device provided in the embodiments of this application;

[0033] Figure 5 This is a partial schematic diagram of the experimental apparatus for mouse social behavior provided in the embodiments of this application;

[0034] Figure 6A fourth-angle structural diagram of the mouse social behavior experimental device provided in the embodiments of this application (cables and relays are not shown).

[0035] Explanation of reference numerals in the attached figures:

[0036] Box body 100; first compartment 110; second compartment 120; third compartment 130; first opening 140; slide 141; second opening 150; bottom plate 160; side wall 170; laser signal collector 200; laser transmitter 210; transmitter end 211; laser receiver 220; receiver end 221; signal output device 300; first partition 400; opening 410; preparation compartment 500; second partition 510; cable 600. Detailed Implementation

[0037] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art based on this application are within the protection scope of the present utility model.

[0038] In order to achieve accurate synchronous labeling of social behavior and calcium signals in experimental mice without the use of cameras and specific behavioral software and without relying on manual intervention, this application provides an experimental device for social behavior in mice, which will be described in detail below.

[0039] The mouse social behavior experimental device in the embodiments of this application, such as Figures 1 to 3 As shown, Figure 1 This is a first-angle structural diagram of the mouse social behavior experimental device provided in an embodiment of this application. Figure 2 This is a second-angle structural diagram of the mouse social behavior experimental device provided in the embodiments of this application. Figure 3 This is a third-angle structural diagram of the mouse social behavior experimental device provided in this application embodiment. The mouse social behavior experimental device includes a box 100, two laser signal collectors 200, and two signal output devices 300. The top of the box 100 is open. The box 100 is divided into a first compartment 110, a second compartment 120, and a third compartment 130 in sequence by two parallel first partitions 400 in the length direction. The bottom of the first partition 400 is provided with an opening 410. The lasers emitted by the two laser signal collectors 200 are horizontally parallel to the first partition 400 and are located in the second compartment 120, close to the first compartment 110 and the third compartment 130, respectively. The two signal output devices 300 are electrically connected to different laser signal collectors 200, respectively.

[0040] Based on the mouse social behavior experimental device provided in the embodiments of this application, mouse social behavior experiments can be conducted using two experimental methods, wherein the first experimental method is:

[0041] Experimental mice, whose calcium signals are collected by a calcium signal recording device, are placed in the second compartment 120. Tool mice are placed in either the first compartment 110 or the third compartment 130. When an experimental mouse passes by the laser signal collector 200 near the compartment containing the tool mouse and blocks the laser emitted by that collector 200, the collector emits a signal indicating that the mouse has moved towards that compartment, thus demonstrating a tendency for the mouse to socialize with the tool mouse. When an experimental mouse passes by the laser signal collector 200 near a compartment without a tool mouse and blocks the laser emitted by that collector... After laser treatment, the laser signal collector 200 emits a signal indicating that the experimental mouse has been detected, representing that the experimental mouse has moved to the compartment, thus indicating that the experimental mouse has exhibited social behavior of resisting social interaction with the tool mouse. The corresponding laser signal collector 200 transmits the corresponding signal of tendency or resistance to social interaction with the tool mouse to the corresponding signal output device 300. The signal output device 300 can output the received signal to the calcium signal recording device, which records the calcium signal at this time. This allows for synchronous labeling of the experimental mouse's social behavior, as indicated by the signal collected by the laser signal collector 200, and the calcium signal of the experimental mouse at the time of the social behavior.

[0042] Experimental Method Two: A mouse whose calcium signal was collected by a calcium signal recording device was placed in the second compartment 120. A familiar tool mouse was placed in one of the first compartments 110 and the third compartment 130, and an unfamiliar tool mouse was placed in the other compartment. When the mouse passed near the laser signal collector 200 in the compartment containing the familiar tool mouse and blocked the laser emitted by that collector 200, the collector emitted a signal indicating that the mouse had moved towards that compartment, thus demonstrating a tendency for the mouse to socialize with the familiar tool mouse. When the mouse passed near the laser signal collector in the compartment containing the unfamiliar tool mouse... After blocking the laser emitted by the laser signal collector 200, the laser signal collector 200 emits a signal indicating that the experimental mouse has moved to the compartment, thus indicating that the experimental mouse has exhibited a social behavior of tending to socialize with unfamiliar tool mice. The corresponding laser signal collector 200 transmits the corresponding signal of tending to socialize with familiar tool mice or unfamiliar tool mice to the corresponding signal output device 300. The signal output device 300 can output the received signal to the calcium signal recording device, which records the calcium signal at this time. This allows for synchronous labeling of the experimental mouse's social behavior as indicated by the signal collected by the laser signal collector 200 and the calcium signal of the experimental mouse at the time of the social behavior.

[0043] Therefore, in the mouse social behavior experimental device provided in this application embodiment, an experimental mouse whose calcium signal is collected by a calcium signal recording device is placed in the second compartment 120, and other tool mice are placed in the first compartment 110 and / or the third compartment 130. When the experimental mouse passes near the laser signal collector 200 in the first compartment 110 or the third compartment 130 and blocks the laser emitted by the laser signal collector 200, the laser signal collector 200 emits a signal indicating that the experimental mouse has moved to that compartment, thereby indicating that the experimental mouse has developed a tendency or resistance to socializing with the tool mice. The laser signal acquisition device 200 transmits the signal to the signal output device 300, which is electrically connected to the laser signal acquisition device 200. The signal output device 300 can output the received signal to the calcium signal recording device. The calcium signal recording device records the calcium signal at this time. This allows the experimental mouse to exhibit social behavior as represented by the signal acquired by the laser signal acquisition device 200, and the calcium signal of the experimental mouse at the time of the social behavior to be synchronously marked. This process does not require manual marking, nor does it require the configuration of a camera or specific behavioral software, and can achieve accurate synchronous marking of the experimental mouse's social behavior and calcium signal.

[0044] In existing technologies, because specific behavior software needs to be used under behavior recording conditions, in order to accurately capture the effect of experimental mice entering a specific area, certain requirements are placed on the lighting of the experimental environment, the placement of the three-box experimental device, and the camera. Shadows must not be generated in the experimental observation area to prevent affecting the specific behavior software's recognition of the signal of the experimental mice entering the specific area. Therefore, the requirements for the experimental environment and operators are relatively high when using specific behavior software. However, the mouse social behavior experimental device provided in this application embodiment does not require manual marking and configuration of professional high-speed cameras, nor does it require specific behavior software. It combines a laser signal acquisition unit 200 and a signal output unit 300 in a single behavior experimental device, which can accurately output signals to mark the synchronously recorded calcium signals when mice enter social areas or non-social areas (or different social areas), solving the problem of high requirements for the experimental environment and the operation of experimental personnel, and making the operation more convenient.

[0045] In some embodiments, the size of the opening 410 of the first partition 400 is set to a size that a mouse cannot pass through.

[0046] In some embodiments, the first partition 400 is a transparent partition, which allows the experimental mice to smell the tool mice while also seeing the tool mice in the first compartment 110 and / or the third compartment 130.

[0047] In some embodiments, the signal output device 300 is a relay. The relay functions as a converter and output signal, specifically converting the signal transmitted from the laser signal acquisition device 200 into a TTL (Transistor-Transistor Logic) signal, and then transmitting the converted TTL signal to the calcium signal recording device. This device, incorporating a relay, can send a TTL signal to the calcium signal recording device when the experimental mouse leans towards the first compartment 110 or the third compartment 130. This facilitates the rapid transmission of the social behavior occurrence time point to the calcium signal recording device, enabling efficient and convenient identification of the calcium signal time point of the social behavior in subsequent analysis. The entire process is simple to implement, not limited by different experimental sites or operator skill levels, and does not require additional specific behavioral software. When analyzing calcium signals, only the acquired TTL signal needs to be imported to accurately obtain the social occurrence time point. Isolating the social occurrence time point facilitates further analysis of calcium signal changes at that time point.

[0048] In the prior art, it is necessary to purchase specific behavioral software and use it in conjunction with a calcium signal recording device. However, the mouse social behavior experimental device provided in this application uses a relay to output a TTL signal to the calcium signal recording device, which can be used in conjunction with various models of calcium signal recording devices to obtain the time point of social behavior and perform rapid analysis of calcium signals.

[0049] In some embodiments, such as Figures 1 to 2 As shown, each laser signal acquisition unit 200 includes a laser emitter 210 and a laser receiver 220, which are electrically connected to the same signal output unit 300. The laser emitter 210 and the laser receiver 220 are respectively located on the outside of two opposite side walls of the housing 100. The two opposite side walls are respectively provided with corresponding second openings 150. The laser emitted by the laser emitter 210 is emitted to the laser receiver 220 through the second openings 150. The two laser signal acquisition units 200 are respectively located close to the first compartment 110 and the third compartment 130 so that the laser emitted by the laser emitter 210 can be horizontally parallel to the first partition 400. When the experimental mouse passes by the laser signal acquisition unit 200 close to the first compartment 110 or the third compartment 130 and blocks the laser emitted by the laser emitter 210, the laser receiver 220 cannot receive the laser. At this time, the laser receiver 220 can emit a signal that the experimental mouse has been detected.

[0050] Specifically, the emitting end 211 of the laser emitter 210 and the receiving end 221 of the laser receiver 220 both face the second opening 150, and the emitting end 211 of the laser emitter 210 and the receiving end 221 of the laser receiver 220 are fixedly connected in the second opening 150.

[0051] In some embodiments, the laser emitter 210 and the laser receiver 220 are the same size, with the laser emitter 210 and the laser receiver 220 having a length of 3 cm and a height of 6.5 cm in the housing 100. The laser emitter 210 and the laser receiver 220 can be gratings.

[0052] In some embodiments, each laser transmitter 210 and each laser receiver 220 are connected to a cable 600, and the laser transmitter 210 and laser receiver 220 in the same laser signal acquisition unit 200 are connected to the corresponding relays through their own cables 600.

[0053] In some embodiments, the outer sheath of each cable is stripped, and the multi-strand cores inside each cable are divided into two groups. The two groups of cores are then wrapped with insulating tape. One group of cores is used to connect to the relay, and the other group is used to connect to the power supply. That is, each laser transmitter and each laser receiver is connected to a power supply separately. When using this device, both laser transmitters and both laser receivers need to be connected to a power supply. Since each laser transmitter and each laser receiver is individually connected to a power supply, if a laser transmitter or laser receiver is damaged and needs to be replaced, only the damaged laser transmitter or laser receiver needs to be replaced, making maintenance and replacement more convenient.

[0054] In some embodiments, such as Figure 4As shown, Figure 4 This is a schematic diagram of the structure of the first partition of the mouse social behavior experimental device provided in the embodiment of this application. The first partition 400 has multiple openings 410. The multiple openings 410 are spaced apart in the width direction of the box 100. The dimension W1 of each opening 410 in the width direction of the box 100 is in the range of 0.35cm≤W1≤0.45cm, and the dimension H1 in the height direction of the box 100 is in the range of 4.7cm≤H1≤4.9cm.

[0055] In this embodiment, multiple openings 410 are provided on the first partition 400, and the multiple openings 410 are spaced apart in the width direction of the box 100, which allows the experimental mice to better smell the tool mice in the first compartment 110 and / or the third compartment 130. In addition, the openings 410 are set to the above-mentioned size, which allows the experimental mice to smell the scent of the tool mice in the first compartment 110 and / or the third compartment 130, but prevents them from entering the first compartment 110 and the third compartment 130, thus preventing the experimental mice from spending too much time in the first compartment 110 and the third compartment 130 and increasing the experimental time.

[0056] The opening 410 at the bottom of the first partition 400 is smaller than that of the experimental mouse and the tool mouse. The experimental mouse cannot crawl into the first compartment 110 or the third compartment 130 through the opening 410 at the bottom of the first partition 400, and the tool mouse cannot crawl out of the first compartment 110 or the third compartment 130 through the opening 410 at the bottom of the first partition 400. The opening 410 at the bottom of the first partition 400 allows the experimental mouse to smell the scent of the tool mouse, but not to directly contact it. This allows for more precise control of the intensity of social interaction between the experimental mouse and the tool mouse, reducing the possibility of aggressive behavior interfering with experimental data.

[0057] In the conventional three-compartment system of the prior art, experimental mice can freely enter the first or third compartment. In addition to social time, the experimental mice may spend some time exploring non-social areas, resulting in low experimental efficiency. The mouse social behavior experimental device of this application isolates the first compartment 110 and the third compartment 130 by means of a first partition 400, with only the second compartment 120 as the social area. This avoids the experimental mice from moving in non-social areas and improves experimental efficiency.

[0058] Specifically, each opening 410 has a dimension W1 of 0.4 cm in the width direction of the box 100 and a dimension H1 of 4.8 cm in the height direction of the box 100.

[0059] In some embodiments, such as Figure 3 and Figure 5 As shown, Figure 5This is a partial schematic diagram of the mouse social behavior experimental device provided in the embodiments of this application. The mouse social behavior experimental device also includes a preparation chamber 500 and a second partition 510. The preparation chamber 500 is located outside the box 100 and is connected to the second compartment 120 through a first opening 140 located on the side wall 170 of the box 100. The first opening 140 is equidistant from the two first partitions 400. The second partition 510 is slidably disposed in the first opening 140.

[0060] In this embodiment, before the experiment begins, the experimental mouse is placed in the preparation chamber 500, and the second partition 510 separates the preparation chamber 500 from the second compartment 120. At the start of the experiment, the second partition 510 is slid upwards along the first opening 140 to release the experimental mouse from the preparation chamber 500. The mouse can then freely enter the second compartment 120 and choose to approach either the first compartment 110 or the second compartment 120. The first opening 140 is located on the side wall of the box 100, and the mouse will enter the second compartment 120 with its head along the width of the box 100. This reduces experimental errors caused by the mouse's head facing either the first compartment 110 or the third compartment 130 when the mouse is placed in the second compartment 120, thus reducing bias caused by human manipulation. The mouse can quickly choose to move towards either the first compartment 110 or the third compartment 130, rapidly reflecting its social orientation and reducing inaccuracies in social behavior detection caused by the mouse exploring other areas.

[0061] Furthermore, the first opening 140 is equidistant from the two first partitions 400, indicating that the initial position of the experimental mouse is equidistant from the first compartment 110 and the third compartment 130. When the experimental mouse is placed in the first compartment 110 and the third compartment 130, the initial position of the experimental mouse will be equidistant from the experimental mouse in the first compartment 110 and the experimental mouse in the third compartment 130, which can further reduce experimental error. The experimental mouse in the preparation chamber 500 is the starting point of the experiment.

[0062] Specifically, such as Figure 5 As shown, the first opening 140 has sliding grooves 141 on both sides in the height direction of the box body 100. The openings of the two sliding grooves 141 are arranged opposite to each other. The second partition 510 is located in the two sliding grooves 141 so that the second partition 510 can slide up and down relative to the first opening 140.

[0063] In some embodiments, such as Figure 3 As shown, the preparation chamber 500 has a length dimension L3 range of 4.8cm≤L3≤5.2cm and a width dimension W4 range of 4.4cm≤W4≤4.6cm, in order to further reduce the size of the mouse social behavior experimental device.

[0064] Specifically, the dimension L3 of the preparation compartment 500 in the length direction of the box 100 is 5cm, and the dimension W4 in the width direction of the box 100 is 4.5cm.

[0065] In some embodiments, such as Figure 3 and Figure 6 As shown, Figure 6 The fourth-angle structural diagram of the mouse social behavior experimental device provided in this application embodiment (cables and relays are not shown) shows the following: the height H2 of the box 100 is in the range of 18cm≤H2≤22cm; the dimensions L1 of the first compartment 110 and the third compartment 130 in the length direction of the box 100 are in the range of 5.8cm≤L1≤6.2cm, and the dimensions W2 in the width direction of the box 100 are in the range of 6.8cm≤W2≤7.2cm; the dimensions L2 of the second compartment 120 in the length direction of the box 100 are in the range of 35cm≤L2≤39cm, and the dimensions W3 in the width direction of the box 100 are in the range of 6.8cm≤W3≤7.2cm.

[0066] In this embodiment, the height H2 of the box 100 is in the range of 18cm≤H2≤22cm. Compared with the 15cm height of the traditional three boxes in the prior art, the height of the box of the mouse social behavior experimental device in this application embodiment is higher, so the environment inside the box 100 is more sealed, which can reduce the anxiety behavior of experimental mice when entering a new environment.

[0067] The conventional three-compartment experimental device of the prior art is generally 45cm long and 30cm wide, and is divided into three compartments along its length. It is relatively large, and the experimental mice can freely enter and exit each compartment. The mouse social behavior experimental device of this application sets the first compartment 110, the third compartment 130 and the second compartment 120 of the compartment to the above dimensions, and the experimental mice cannot enter the first compartment 110 and the third compartment 130, which improves experimental efficiency, avoids the activity of experimental mice in non-social areas, and improves the observation efficiency of social behavior.

[0068] Specifically, the height H2 of the box body 100 can be 20cm, and the heights of the first partition 400 and the second partition 510 can be the same as the height of the box body 100. The first compartment 110 and the third compartment 130 have a length dimension L1 of 6cm and a width dimension W2 of 7cm in the box body 100; the second compartment 120 has a length dimension L2 of 37cm and a width dimension W3 of 7cm in the box body 100.

[0069] In some embodiments, such as Figure 2As shown, the bottom plate 160 and side wall 170 of the housing 100 are detachably connected, which facilitates cleaning the inside of the housing 100.

[0070] Specifically, the bottom plate 160 of the enclosure 100 can be a movable partition, the top of the bottom plate 160 can be provided with a positioning hole, and the bottom of the side wall 170 is provided with a positioning post corresponding to the positioning hole. The bottom plate 160 and the side wall 170 of the enclosure 100 can be positioned and detachably connected through the positioning hole and the positioning post.

[0071] In some embodiments, the sidewall 170 of the housing 100 may also be placed directly on the base plate 160 to facilitate cleaning of the inside of the housing 100.

[0072] The above description is merely a preferred embodiment of this utility model and is not intended to limit the scope of protection of this utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model are included within the scope of protection of this utility model.

Claims

1. A mouse social behavior experimental device, characterized in that, include: The box has an open top and is divided into a first compartment, a second compartment, and a third compartment in sequence by two parallel first partitions along its length; the first partitions have openings at their bottoms. Two laser signal collectors emit lasers that are horizontally parallel to the first partition and are located in the second compartment, respectively close to the first compartment and the third compartment; Two signal output devices are provided, each electrically connected to a different laser signal acquisition device.

2. The experimental apparatus for mouse social behavior according to claim 1, characterized in that, Also includes: A preparation compartment is located outside the box body and is connected to the second compartment through a first opening located on the side wall of the box body. The first opening is equidistant from the two first partitions. The second partition is slidably disposed in the first opening.

3. The experimental apparatus for mouse social behavior according to claim 1, characterized in that, Each laser signal acquisition unit includes: a laser transmitter and a laser receiver, both electrically connected to the same signal output unit; The laser emitter and the laser receiver are respectively located on the outside of two opposite side walls of the housing. The two opposite side walls are respectively provided with corresponding second openings. The laser emitted by the laser emitter is emitted to the laser receiver through the second openings.

4. The experimental apparatus for mouse social behavior according to claim 1, characterized in that, The first partition is a transparent partition.

5. The experimental apparatus for mouse social behavior according to claim 1, characterized in that, The first partition has multiple openings; The plurality of openings are spaced apart in the width direction of the housing; For each of the aforementioned openings, the dimension W1 in the width direction of the box body is in the range of 0.35cm≤W1≤0.45cm, and the dimension H1 in the height direction of the box body is in the range of 4.7cm≤H1≤4.9cm.

6. The experimental apparatus for mouse social behavior according to claim 1, characterized in that, The height H2 of the box body is in the range of: 18cm≤H2≤22cm; The first compartment and the third compartment have dimensions L1 ranging from 5.8cm to 6.2cm in the length direction of the box body, and dimensions W2 ranging from 6.8cm to 7.2cm in the width direction of the box body. The second compartment has a length L2 range of 35cm≤L2≤39cm and a width W3 range of 6.8cm≤W3≤7.2cm.

7. The experimental apparatus for mouse social behavior according to claim 2, characterized in that, The preparation compartment has a length L3 range of 4.8cm ≤ L3 ≤ 5.2cm and a width W4 range of 4.4cm ≤ W4 ≤ 4.6cm.

8. The experimental apparatus for mouse social behavior according to claim 2, characterized in that, The first opening has grooves on both sides in the height direction of the box, the openings of the two grooves are arranged opposite to each other, and the second partition is located in the two grooves.

9. The experimental apparatus for mouse social behavior according to claim 1, characterized in that, The signal output device is a relay.

10. The experimental apparatus for mouse social behavior according to claim 1, characterized in that, The bottom plate and side walls of the enclosure are detachably connected.

Images