A fluid separation system

Abstract

The utility model discloses a kind of separation fluid systems. Separation fluid system includes pipeline consumable module, liquid storage module, control valve module, power module and centrifuge module, pipeline consumable module has multiple pipe orifices, liquid storage module has multiple storage objects, and different storage objects are used to store different kinds of liquid in different storage objects. The separation fluid system can make the liquid of storage object flow according to preset timing and preset path, to perform pipeline rinse operation, separation liquid loading operation, sample loading operation, centrifugation object storage piece cleaning operation, intermediate loading operation, sample cleaning operation, cryopreservation liquid replacement operation, and target output operation, so as to realize the separation of target in liquid sample, and finally store target to target storage piece. The separation fluid system can realize the automatic separation of target in liquid sample without the help of manual operation, not only has good separation effect, low probability of being contaminated, but also has high separation efficiency and high repeatability.

Description

Technical Field

[0001] This utility model relates to the field of separation system technology, and in particular to a fluid separation system. Background Technology

[0002] With breakthroughs in technologies such as gene editing and regenerative medicine, the application scope of cell storage continues to expand, providing new safeguards for disease treatment and health management. In the process of storing mononuclear cells from peripheral blood or umbilical cord blood, the mononuclear cells must first be isolated from the peripheral blood or umbilical cord blood, then concentrated to a set volume, resuspended in a certain amount of cryopreservation solution, and finally transferred to the target bag for subsequent storage or research.

[0003] Currently, the separation of mononuclear cells in the industry generally requires manual operation, which not only demands high levels of personnel skill and a suitable operating environment, but also suffers from low efficiency and instability. For cell storage operations implemented in different regions, the manual operation model struggles to provide higher returns. Taking the separation of mononuclear cells from umbilical cord blood as an example, while existing technologies and extraction devices exist, they still suffer from the following drawbacks: ① Steps requiring manual operation, such as manually operating centrifuges and manually performing sedimentation and stratification, demand high levels of operator skill; ② Umbilical cord blood is exposed to air throughout the process, requiring a high level of environmental control and posing a significant risk of contamination; ③ Low operational efficiency hinders industrial-scale promotion. Utility Model Content

[0004] The purpose of this invention is to provide a fluid separation system that can automatically separate target substances from liquid samples, with good separation effect, low probability of contamination, high separation efficiency, and high repeatability.

[0005] To achieve this objective, the present invention adopts the following technical solution:

[0006] A fluid separation system includes: a tubing consumable module having multiple ports, including a buffer solution port, a cryopreservation solution port, a liquid sample port, a separation solution port, a centrifuge port, an intermediate solution port, a waste liquid port, and a destination solution port; a storage module having multiple storage units, including buffer solution storage units, cryopreservation solution storage units, liquid sample storage units, separation solution storage units, centrifuge solution storage units, intermediate solution storage units, waste liquid storage units, and destination solution storage units, which are correspondingly installed on the buffer solution port, the cryopreservation solution port, the liquid sample port, the separation solution port, the centrifuge port, the intermediate solution port, the waste liquid port, and the destination solution port; and a control valve module disposed on the tubing consumable module. The system includes a block and the ability to selectively control the connection of at least two of the storage units; a power module, located on the pipeline consumable module, for providing power for the flow of liquid between the storage units; and a centrifuge module, located at the centrifuge storage unit, for centrifuging and mixing the liquid within the centrifuge storage unit. The separation fluid system is configured to allow the liquid in the storage units to flow according to a preset sequence and path, performing pipeline rinsing, separation liquid loading, sample loading, centrifuge storage unit cleaning, intermediate loading, sample cleaning, cryopreservation solution replacement, and target substance output operations, thereby separating the target substance from the liquid sample and storing it in the target substance storage unit.

[0007] Preferably, the fluid separation system further includes a color detection mechanism, which is located on the centrifuge pipeline and is used to obtain the color of the liquid discharged from the centrifuge storage container. The end of the centrifuge pipeline is connected to the outlet of the centrifuge storage container.

[0008] Preferably, the fluid separation system further includes a first bubble detection element, which is disposed on the centrifuge pipeline, and the end of the centrifuge pipeline is connected to the outlet of the centrifuge storage container.

[0009] Preferably, the fluid separation system further includes a second bubble detection element, which is disposed on the first main pipeline. The first main pipeline is connected to the output port of the buffer solution storage container, the output port of the cryopreservation solution storage container, the output port of the liquid sample storage container, and the output port of the separation solution storage container.

[0010] Preferably, the fluid separation system further includes a weighing mechanism, on which the target material storage container is placed, and the weighing mechanism is used to obtain the weight of the target material in the target material storage container; or, the fluid separation system further includes a flow detection mechanism, which is located at the target material inlet and is used to obtain the cumulative flow of the target material entering the target material storage container.

[0011] Preferably, the separation fluid system further comprises a first pressure detection element and a second pressure detection element, wherein the first pressure detection element is configured to acquire a pressure at an input end of the power module, and the second pressure detection element is configured to acquire a pressure at an output end of the power module.

[0012] Preferably, the power module is a peristaltic pump.

[0013] Preferably, the separation fluid system further comprises a rotary joint, and the centrifugate storage member is rotatably mounted at the centrifugal pipe opening via the rotary joint; and / or, the separation fluid system further comprises a filter, wherein the filter is configured to filter buffer flowing out of the buffer storage member; and / or, the separation fluid system further comprises a puncture member, wherein the puncture member is configured to pierce into the buffer storage member.

[0014] Preferably, the pipeline consumable module comprises a power pipeline and an annular pipeline which are communicated in a "ri" (Chinese character for sun) shape, the power module is arranged on the power pipeline, and all the storage members are communicated with the annular pipeline.

[0015] Preferably, the annular pipeline comprises a first main pipeline, a second main pipeline, a third main pipeline, a fourth main pipeline, a fifth main pipeline and a sixth main pipeline which are communicated end to end, the second main pipeline and the third main pipeline have a first communication point, the third main pipeline and the fourth main pipeline have a second communication point, the fifth main pipeline and the sixth main pipeline have a third communication point, the sixth main pipeline and the first main pipeline have a fourth communication point; the pipeline consumable module further comprises a centrifugal pipeline, a waste discharge pipeline, a target product pipeline, a buffer pipeline, a cryopreservation pipeline, a sample pipeline, a separation pipeline and an intermediate product pipeline, one end of the centrifugal pipeline is communicated with the second communication point, and the other end forms the centrifugal pipe opening; one end of the waste discharge pipeline is communicated with the third main pipeline, and the other end forms a waste liquid pipe opening; one end of the target product pipeline is communicated with the first communication point, and the other end forms a target product pipe opening; one end of the buffer pipeline is communicated with the third communication point, and the other end forms a buffer liquid pipe opening; one end of the cryopreservation pipeline is communicated with the sixth main pipeline, and the other end forms a cryopreservation liquid pipe opening; one end of the sample pipeline is communicated with the sixth main pipeline, and the other end forms a liquid sample pipe opening; one end of the separation pipeline is communicated with the sixth main pipeline, and the other end forms a separation liquid pipe opening; one end of the intermediate product pipeline is communicated with the fourth communication point, and the other end forms an intermediate product pipe opening; and / or, the pipeline consumable module further comprises a standby pipeline, one end of the standby pipeline is communicated with the sixth main pipeline, and the other end forms a standby pipe opening.

[0016] Preferably, the control valve module includes a first control valve on the buffer line, a second control valve on the spare line, a third control valve on the cryopreservation line, a fourth control valve on the sixth main line, a fifth control valve on the sample line, a sixth control valve on the separation line, a seventh control valve on the intermediate material line, an eighth control valve on the second main line, a ninth control valve on the destination material line, a tenth control valve on the waste discharge line, an eleventh control valve on the centrifugation line, a twelfth control valve on the third main line, a thirteenth control valve on the fourth main line, and a fourteenth control valve on the fifth main line.

[0017] Preferably, the fluid separation system has a first waste discharge channel, which includes a centrifugal pipeline, a portion of a third main pipeline, and a waste discharge pipeline connected in sequence. The end of the centrifugal pipeline away from the third main pipeline forms the centrifugal port, and the end of the waste discharge pipeline away from the third main pipeline forms the waste liquid port. An eleventh control valve is provided on the centrifugal pipeline, a twelfth control valve is provided on the third main pipeline, and a tenth control valve is provided on the waste discharge pipeline.

[0018] Preferably, the fluid separation system has a second waste discharge channel, which includes a centrifugal pipeline, a fourth main pipeline, a power pipeline, a second main pipeline, a portion of a third main pipeline, and a waste discharge pipeline connected in sequence. The centrifugal pipeline's end away from the fourth main pipeline forms the centrifugal port, and the waste discharge pipeline's end away from the third main pipeline forms the waste liquid port. The power module is located on the power pipeline. The centrifugal pipeline is equipped with an eleventh control valve, the fourth main pipeline with a thirteenth control valve, the second main pipeline with an eighth control valve, and the waste discharge pipeline with a tenth control valve.

[0019] The beneficial effects of this utility model are:

[0020] This utility model provides a fluid separation system comprising a pipeline consumable module, a liquid storage module, a control valve module, a power module, and a centrifuge module. The pipeline consumable module has multiple ports, and the liquid storage module has multiple storage containers, each storing different types of liquids. This fluid separation system enables the liquid in the storage containers to flow according to a preset sequence and path to perform pipeline rinsing, separation liquid loading, sample loading, centrifuge container cleaning, intermediate loading, sample cleaning, cryopreservation solution replacement, and target analyte output operations. This achieves the separation of the target analyte from the liquid sample and ultimately stores it in the target analyte storage container. This fluid separation system can automatically separate the target analyte from the liquid sample without manual operation, offering excellent separation results, low contamination risk, high separation efficiency, and high repeatability. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the pipeline consumable module and the liquid storage module of the fluid separation system provided by this utility model;

[0022] Figure 2 This is a schematic diagram of the fluid separation system provided by this utility model;

[0023] Figure 3 This is a pressure change trend diagram provided by this utility model.

[0024] In the picture:

[0025] 100. Piping Consumables Module; 101. Buffer Solution Port; 102. Cryopreservation Solution Port; 103. Liquid Sample Port; 104. Separation Solution Port; 105. Centrifuge Port; 106. Intermediate Material Port; 107. Waste Liquid Port; 108. Destination Material Port; 109. Power Piping; 110. First Main Piping; 111. Second Main Piping; 112. Third Main Piping; 113. Fourth Main Piping; 114. Fifth Main Piping; 115. Sixth Main Piping; 116. Centrifuge Piping; 117. Waste Discharge Piping; 118. Destination Material Piping; 119. Buffer Piping; 120. Cryopreservation Piping; 121. Sample Piping; 122. Separation Piping; 123. Intermediate Material Piping; 124. Spare Piping; 125. Spare Port;

[0026] 200. Liquid storage module; 201. Buffer solution storage unit; 202. Cryopreservation solution storage unit; 203. Liquid sample storage unit; 204. Separation solution storage unit; 205. Centrifuged material storage unit; 206. Intermediate material storage unit; 207. Waste liquid storage unit; 208. Destination material storage unit;

[0027] 300. Control valve module; 301. First control valve; 302. Second control valve; 303. Third control valve; 304. Fourth control valve; 305. Fifth control valve; 306. Sixth control valve; 307. Seventh control valve; 308. Eighth control valve; 309. Ninth control valve; 310. Tenth control valve; 311. Eleventh control valve; 312. Twelfth control valve; 313. Thirteenth control valve; 314. Fourteenth control valve;

[0028] 400. Power module;

[0029] 500. Centrifuge module;

[0030] 610. Color detection mechanism; 620. First bubble detection component; 630. Second bubble detection component; 640. Weighing mechanism; 650. First pressure detection component; 660. Second pressure detection component;

[0031] 710. Rotary joint; 720. Filter; 730. Puncture device;

[0032] 800. System Framework;

[0033] 900. Display screen. Detailed Implementation

[0034] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present invention and not intended to limit it. Furthermore, it should be noted that, for ease of description, the accompanying drawings show only the parts relevant to the present invention, not the entire structure.

[0035] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0036] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0037] In the description of this embodiment, the terms "upper," "lower," "right," etc., refer to the orientation or positional relationship shown in the accompanying drawings. They are used only for ease of description and simplification of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are only used for distinction in description and have no special meaning.

[0038] This invention discloses a fluid separation system applicable to the biological field, capable of fully automated separation of mononuclear cells from biological samples, particularly suitable for the separation of mononuclear cells from peripheral blood, umbilical cord blood, and tissue samples. It can also be used for the separation of other cells, biological products, etc. Besides the biological field, this fluid separation system can also be used for the component separation of multi-component liquids in other industrial fields. For ease of description, the samples requiring separation using the fluid separation system are collectively referred to as liquid samples, the desired final product as the target substance, and the intermediate products carrying the target substance in intermediate processing steps as intermediates.

[0039] like Figure 1 and Figure 2As shown, the fluid separation system includes a tubing consumable module 100, a liquid storage module 200, a control valve module 300, a power module 400, and a centrifuge module 500. The tubing consumable module 100 has multiple ports, including a buffer solution port 101, a cryopreservation solution port 102, a liquid sample port 103, a separation solution port 104, a centrifuge port 105, an intermediate solution port 106, a waste liquid port 107, and a destination solution port 108. The liquid storage module 200 has multiple storage components, including a buffer solution storage unit 201, a cryopreservation solution storage unit 202, a liquid sample storage unit 203, a separation solution storage unit 204, a centrifuge sample storage unit 205, an intermediate solution storage unit 206, a waste liquid storage unit 207, and a target substance storage unit 208. The buffer solution storage unit 201 is installed at the buffer solution port 101, the cryopreservation solution storage unit 202 is installed at the cryopreservation solution port 102, the liquid sample storage unit 203 is installed at the liquid sample port 103, the separation solution storage unit 204 is installed at the separation solution port 104, the centrifuge sample storage unit 205 is installed at the centrifuge port 105, the intermediate solution storage unit 206 is installed at the intermediate solution port 106, the waste liquid storage unit 207 is installed at the waste liquid port 107, and the target substance storage unit 208 is installed at the target substance port 108. A control valve module 300 is located on the pipeline consumable module 100 and can selectively select at least two storage units and control their connection. A power module 400 is located on the pipeline consumable module 100 and is used to provide power for the flow of liquid between the connected storage units. A centrifuge module 500 is located at the centrifuge material storage unit 205 and is used to centrifuge and mix the liquid within the centrifuge material storage unit 205. It should be noted that the storage unit can be a storage bag, a storage bottle, or other storage containers. In one embodiment, the buffer solution storage unit 201 is a buffer solution bag, the cryopreservation solution storage unit 202 is a cryopreservation solution bag, the liquid sample storage unit 203 is a sample bag, the separation solution storage unit 204 is a separation solution bag, the centrifuge material storage unit 205 is a centrifuge bag, the intermediate material storage unit 206 is an intermediate material bag, the waste liquid storage unit 207 is a waste liquid bag, and the destination material storage unit 208 is a destination bag.

[0040] This fluid separation system enables the liquid in the storage container to flow according to a preset sequence and path to perform pipeline rinsing, separation liquid loading, sample loading, centrifuge storage container cleaning, intermediate loading, sample cleaning, cryopreservation solution replacement, and target analyte output operations, thereby separating the target analyte from the liquid sample and storing it in the target analyte storage container 208. It should be noted that for a single liquid sample separation experiment, the aforementioned operations can be performed sequentially in the order of "pipeline rinsing, separation liquid loading, sample loading, centrifuge storage container cleaning, intermediate loading, sample cleaning, cryopreservation solution replacement, and target analyte output operation," or they can be performed without strictly adhering to this sequence; for example, the order of separation liquid loading and sample loading operations can be interchanged.

[0041] Specifically, during the pipeline rinsing operation, the buffer solution in the buffer storage member 201 flows through all common pipelines of the pipeline consumable module 100, so as to rinse each common pipeline in the pipeline consumable module 100. During the separation solution loading operation, the separation solution in the separation solution storage member 204 flows into the centrifugate storage member 205. During the sample loading operation, the following steps are performed: the liquid sample in the liquid sample storage member 203 flows into the centrifugate storage member 205; the buffer solution in the buffer storage member 201 flows into the liquid sample storage member 203 to clean residues and the resulting solution is discharged into the centrifugate storage member 205; the mixture in the centrifugate storage member 205 is rotated and centrifuged to form an intermediate; the intermediate in the centrifugate storage member 205 flows into the intermediate storage member 206; and the waste liquid in the centrifugate storage member 205 flows into the waste liquid storage member 207. During the centrifugate storage member cleaning operation, the buffer solution in the buffer storage member 201 flows into the centrifugate storage member 205 for cleaning, and the waste liquid is discharged to the waste liquid storage member 207. The cleaning times can be set to multiple times according to requirements to improve the cleaning effect. During the intermediate loading operation, the intermediate in the intermediate storage member 206 flows into the centrifugate storage member 205. During the sample cleaning operation, the following steps are performed: the buffer solution in the buffer storage member 201 flows into the centrifugate storage member 205; the mixture in the centrifugate storage member 205 is rotated and centrifuged; and the waste liquid in the centrifugate storage member 205 is discharged to the waste liquid storage member 207. During the cryopreservation solution replacement operation, the following steps are performed: the cryopreservation solution in the cryopreservation solution storage member 202 flows into the centrifugate storage member 205; the mixture in the centrifugate storage member 205 is rotated and centrifuged again; and the waste liquid in the centrifugate storage member 205 is discharged to the waste liquid storage member 207 again. During the target product output operation, the remaining target product in the centrifugate storage member 205 flows into the target product storage member 208.

[0042] The pipeline consumable module 100 is a disposable pipeline consumable, with further reference to Figure 1 shown, in some embodiments, the pipeline consumable module 100 comprises a central pipeline assembly (i.e., a common pipeline), the central pipeline assembly comprises a power pipeline 109 and an annular pipeline which communicate in a "ri" (Japanese-like) shape, the power pipeline 109 communicates within the annular pipeline, the power module 400 is arranged on the power pipeline 109, and all the storage members communicate with the annular pipeline.

[0043] In some embodiments, the annular pipeline comprises a first main pipeline 110, a second main pipeline 111, a third main pipeline 112, a fourth main pipeline 113, a fifth main pipeline 114 and a sixth main pipeline 115 which are communicated end to end. The second main pipeline 111 and the third main pipeline 112 have a first communication point, as shown in Figure 1 indicated by A therein, the third main pipeline 112 and the fourth main pipeline 113 have a second communication point, as shown in Figure 1 indicated by B therein, the fifth main pipeline 114 and the sixth main pipeline 115 have a third communication point, as shown in Figure 1 indicated by C therein, the sixth main pipeline 115 and the first main pipeline 110 have a fourth communication point, as shown in Figure 1 As shown in D.

[0044] Continue to refer to Figure 1 As shown, the tubing consumable module 100 also includes centrifuge tubing 116, waste discharge tubing 117, target material tubing 118, buffer tubing 119, cryopreservation tubing 120, sample tubing 121, separation tubing 122, and intermediate material tubing 123. One end of centrifuge tubing 116 is connected to a second connection point, and the other end of centrifuge tubing 116 forms a centrifuge port 105. One end of waste discharge tubing 117 is connected to a third main tubing 112, and the other end of waste discharge tubing 117 forms a waste liquid port 107. One end of target material tubing 118 is connected to a first connection point, and the other end of target material tubing 118 forms a target material port 108. One end of buffer tubing 119 is connected to a third connection point, and the other end of buffer tubing 119 forms a buffer solution port 101. One end of cryopreservation tubing 120 is connected to a sixth main tubing 115, and the other end of cryopreservation tubing 120 forms a cryopreservation liquid port 102. One end of the sample line 121 is connected to the sixth main line 115, and the other end of the sample line 121 forms a liquid sample port 103. One end of the separation line 122 is connected to the sixth main line 115, and the other end of the separation line 122 forms a separation liquid port 104. One end of the intermediate material line 123 is connected to the fourth connection point, and the other end of the intermediate material line 123 forms an intermediate material port 106.

[0045] Continue to refer to Figure 1 As shown, the tubing consumable module 100 also includes a spare tubing 124. One end of the spare tubing 124 is connected to the sixth main tubing 115, and the other end forms a spare port 125. Regarding the spare tubing 124, the spare port 125 formed therein can be used as any one of the buffer solution port 101, cryopreservation solution port 102, liquid sample port 103, separation solution port 104, and intermediate solution port 106, to replace any one of the buffer tubing 119, cryopreservation tubing 120, sample tubing 121, separation tubing 122, and intermediate solution tubing 123 in case of failure, thereby improving the reliability of the separation fluid system.

[0046] Of course, in other embodiments, the tubing consumable module 100 may be configured with other shapes as needed, as long as it does not affect the separation fluid system's performance of tubing rinsing, separation liquid loading, sample loading, centrifuge storage cleaning, intermediate loading, sample cleaning, cryopreservation solution replacement, and target product output operations. Furthermore, all tubing included in the tubing consumable module 100 can be detachably connected via pipe fittings, which can be either tee fittings or two-way fittings as required.

[0047] Continue to refer to Figure 2As shown, the control valve module 300 includes a first control valve 301 located on the buffer line 119, a second control valve 302 located on the backup line 124, a third control valve 303 located on the cryopreservation line 120, a fourth control valve 304 located on the sixth main line 115, a fifth control valve 305 located on the sample line 121, a sixth control valve 306 located on the separation line 122, and a seventh control valve 304 located on the intermediate material line 123. 7. The following valves are provided: an eighth control valve 308 on the second main pipeline 111, a ninth control valve 309 on the destination pipeline 118, a tenth control valve 310 on the waste discharge pipeline 117, an eleventh control valve 311 on the centrifugal pipeline 116, a twelfth control valve 312 on the third main pipeline 112, a thirteenth control valve 313 on the fourth main pipeline 113, and a fourteenth control valve 314 on the fifth main pipeline 114. By controlling the opening and closing of these control valves according to a preset time sequence, the connection of each pipeline can be controlled for a preset time period, thereby controlling the flow of liquid in each storage container according to a preset path. Optionally, each of the above control valves is a solenoid valve.

[0048] It should be noted that, in the embodiments of this utility model, in the fluid separation system, from the centrifuge storage unit 205 to the waste liquid storage unit 207, there are two waste discharge channels: a first waste discharge channel and a second waste discharge channel. The first waste discharge channel is a compression waste discharge channel, which includes a centrifuge pipe 116, a portion of a third main pipe 112, and a waste discharge pipe 117 connected in sequence. The end of the centrifuge pipe 116 away from the third main pipe 112 forms a centrifuge port 105, and the end of the waste discharge pipe 117 away from the third main pipe 112 forms a waste liquid port 107. An eleventh control valve 311 is provided on the centrifuge pipe 116, a twelfth control valve 312 is provided on the third main pipe 112, and a tenth control valve 310 is provided on the waste discharge pipe 117.

[0049] The second waste discharge channel is a pump suction waste discharge channel. The second waste discharge channel includes a centrifugal pipeline 116, a fourth main pipeline 113, a power pipeline 109, a second main pipeline 111, a portion of a third main pipeline 112, and a waste discharge pipeline 117 connected in sequence. The end of the centrifugal pipeline 116 away from the fourth main pipeline 113 forms a centrifugal port 105, and the end of the waste discharge pipeline 117 away from the third main pipeline 112 forms a waste liquid port 107. The power module 400 is installed on the power pipeline 109. The centrifugal pipeline 116 is equipped with an eleventh control valve 311, the fourth main pipeline 113 is equipped with a thirteenth control valve 313, the second main pipeline 111 is equipped with an eighth control valve 308, and the waste discharge pipeline 117 is equipped with a tenth control valve 310.

[0050] It should be noted that when performing the above operations, if waste discharge from the centrifugal material storage unit 205 to the waste liquid storage unit 207 is involved, either the first waste discharge channel or the second waste discharge channel can be used alone. Alternatively, the first and second waste discharge channels can be used sequentially; for example, the first waste discharge channel can be used for compression waste discharge first, followed by pumping waste discharge using the second waste discharge channel. This dual waste discharge offers greater flexibility and ensures more thorough waste discharge.

[0051] In detail, during the pipeline flushing operation, the first control valve 301, the fourteenth control valve 314, the eighth control valve 308, and the tenth control valve 310 are first opened for a period of time, while other control valves are closed. This allows the buffer solution in the buffer reservoir 201 to flow through the buffer pipeline 119, the fifth main pipeline 114, the power pipeline 109, the second main pipeline 111, part of the third main pipeline 112, and the waste discharge pipeline 117. Then, the first control valve 301, the fourth control valve 304, the thirteenth control valve 313, the twelfth control valve 312, and the tenth control valve 310 are opened for a period of time, while other control valves are closed. This allows the buffer solution in the buffer reservoir 201 to flow through the buffer pipeline 119, the sixth main pipeline 115, the first main pipeline 110, the power pipeline 109, the fourth main pipeline 113, the remaining third main pipeline 112, and the waste discharge pipeline 117, thereby achieving flushing of the entire central pipeline assembly.

[0052] When performing the separation liquid loading operation, the sixth control valve 306, the thirteenth control valve 313 and the eleventh control valve 311 are opened, and other control valves are closed, so that the separation liquid in the separation liquid storage 204 flows through the separation pipeline 122, part of the sixth main pipeline 115, the first main pipeline 110, the power pipeline 109, the fourth main pipeline 113 and the centrifugal pipeline 116, thereby realizing the loading of separation liquid in the centrifugal storage 205.

[0053] During the sample loading operation, the fifth control valve 305, the thirteenth control valve 313, and the eleventh control valve 311 are first opened for a period of time, while other control valves are closed. This allows the liquid sample in the liquid sample storage container 203 to flow through the sample pipeline 121, part of the sixth main pipeline 115, the first main pipeline 110, the power pipeline 109, the fourth main pipeline 113, and the centrifuge pipeline 116, thereby loading the liquid sample in the centrifuge storage container 205. Then, the thirteenth control valve 313 and the eleventh control valve are closed. 311, then control the first control valve 301 and the fourth control valve 304 to open, so that the buffer solution in the buffer solution reservoir 201 flows into the liquid sample reservoir 203 through the buffer line 119, part of the sixth main line 115 and the sample line 121 to rinse the liquid sample reservoir 203 of residual liquid sample; then, close the first control valve 301 and the fourth control valve 304, and then open the thirteenth control valve 313 and the eleventh control valve 311 to rinse the buffer solution and liquid sample residue after rinsing the liquid sample reservoir 203. The mixture flows through sample line 121, part of sixth main line 115, first main line 110, power line 109, fourth main line 113, and centrifuge line 116 to centrifuge storage unit 205. Then, centrifuge module 500 drives centrifuge storage unit 205 to rotate, causing the mixture inside to rotate. Under centrifugal force, the separated liquid and liquid sample in centrifuge storage unit 205 will separate into layers along the radius of centrifuge storage unit 205, forming intermediates and waste liquid. Then, the eleventh control... Control valves 311, 12th control valve 312, 8th control valve 308, and 7th control valve 307 are opened for a period of time, while other control valves are closed, allowing the intermediate material in centrifuge storage 205 to flow into intermediate material storage 206. Finally, control valves 11th control valve 311, 13th control valve 313, 8th control valve 308, and 10th control valve 310 are opened for a period of time (i.e., using the second waste discharge channel), while other control valves are closed, allowing the waste liquid in centrifuge storage 205 to flow into waste liquid storage 207. Of course, the last step of this operation can also use the first waste discharge channel; or a dual waste discharge method can be used, i.e., first using the first waste discharge channel for squeezing waste discharge, and then using the second waste discharge channel for pumping waste discharge.

[0054] During the centrifuge storage unit cleaning operation, the first control valve 301, the fourth control valve 304, the thirteenth control valve 313, and the eleventh control valve 311 are opened for a period of time, while other control valves are closed. This allows the buffer solution in the buffer storage unit 201 to flow through the buffer pipeline 119, the sixth main pipeline 115, the first main pipeline 110, the power pipeline 109, the fourth main pipeline 113, and the centrifuge pipeline 116, thereby cleaning the centrifuge storage unit 205. Then, the eleventh control valve 311, the thirteenth control valve 313, the eighth control valve 308, and the tenth control valve 310 are opened for a period of time (i.e., using the second waste discharge channel), while other control valves are closed, allowing the cleaning waste liquid in the centrifuge storage unit 205 to flow into the waste liquid storage unit 207. Alternatively, the final step of this operation can also utilize the first waste discharge channel; or a dual waste discharge method can be used, where the first waste discharge channel is first used for squeezing waste discharge, followed by pumping waste discharge using the second waste discharge channel. This operation can be repeated multiple times as needed to achieve multiple cleanings of the centrifugal storage container 205.

[0055] In other embodiments, after performing the sample loading operation, a common pipeline cleaning operation is also included, which can be specifically performed as described above in terms of pipeline rinsing.

[0056] During the intermediate loading operation, the seventh control valve 307, the thirteenth control valve 313, and the eleventh control valve 311 are opened for a period of time, and other control valves are closed, so that the intermediate in the intermediate storage 206 flows through the intermediate pipeline 123, the first main pipeline 110, the power pipeline 109, the fourth main pipeline 113, and the centrifugal pipeline 116, so as to load the intermediate in the intermediate storage 206 into the centrifugal storage 205.

[0057] During the cryopreservation fluid replacement operation, the first control valve 301, the fourth control valve 304, the thirteenth control valve 313, and the eleventh control valve 311 are first opened for a period of time, while other control valves are closed. This allows the buffer solution in the buffer solution reservoir 201 to flow through the buffer line 119, the sixth main line 115, the first main line 110, the power line 109, the fourth main line 113, and the centrifuge line 116, thereby loading the buffer solution into the centrifuge material reservoir 205. Then, all the aforementioned control valves are closed, and the centrifuge module 500 is started. The centrifuge module 500 drives the centrifuge material reservoir 205 to rotate, causing the mixture in the centrifuge material reservoir 205 to rotate. Under the action of centrifugal force, the mixture will stratify along the radial direction of the centrifuge material reservoir 205, which can achieve the cleaning of intermediate materials and generate waste liquid. Then, the eleventh control valve 311, the twelfth control valve 312, and the tenth control valve 310 are opened for a period of time to discharge the waste liquid generated in the previous step into the waste liquid reservoir 207. It should be noted that the final step of this operation, which uses the first waste discharge channel for squeezing and waste discharge, has the advantage of not requiring pipeline cleaning.

[0058] During the cryopreservation fluid replacement operation, the third control valve 303, the fourth control valve 304, the thirteenth control valve 313, and the eleventh control valve 311 are opened for a period of time, while other control valves are closed. This allows the cryopreservation fluid in the cryopreservation fluid storage unit 202 to flow through the cryopreservation pipeline 120, part of the sixth main pipeline 115, the first main pipeline 110, the power pipeline 109, the fourth main pipeline 113, and the centrifuge pipeline 116, thereby loading the cryopreservation fluid into the centrifuge material storage unit 205. Then, all the aforementioned control valves are closed, and the centrifuge module 500 drives the centrifuge material storage unit 205 to rotate, causing the mixture in the centrifuge material storage unit 205 to rotate and centrifuge to form the target material and waste liquid. Finally, the waste liquid is discharged. It should be noted that the waste discharge here can be performed by squeezing the waste through the first waste discharge channel, or by pumping the waste through the second waste discharge channel; or a dual waste discharge can be used, that is, squeezing the waste through the first waste discharge channel first, and then pumping the waste through the second waste discharge channel.

[0059] When performing the target material output operation, the centrifuge module 500 is first controlled to perform forward and reverse rotation operations on the centrifuge material storage unit 205, so that the target material in the centrifuge material storage unit 205 flows from the periphery to the central area; then the eleventh control valve 311, the thirteenth control valve 313, the eighth control valve 308 and the ninth control valve 309 are controlled to open for a period of time, and other control valves are controlled to close, and the power module 400 draws the target material into the target material storage unit 208 to realize the storage of the target material.

[0060] Continue to refer to Figure 2As shown, the fluid separation system also includes a color detection mechanism 610, which is located at the outlet or downstream of the centrifugal storage unit 205 and is used to obtain the color of the liquid discharged from the centrifugal storage unit 205. By setting the color detection mechanism 610, the color of the liquid output from the centrifugal storage unit 205 can be determined, thereby determining whether a certain component has been input or output completely.

[0061] Optionally, the color detection mechanism 610 is a color sensor. In one specific embodiment, the color sensor is located on the centrifuge tube 116. For example, when the liquid sample is umbilical cord blood or peripheral blood, the color sensor can detect the color change of the liquid in real time when the intermediate is discharged, thereby reducing the amount of red blood cells discharged into the intermediate storage container 206 during the mononuclear cell output process and ensuring the purity of the mononuclear cells in the intermediate storage container 206. Of course, in addition to using a color sensor, the color detection mechanism 610 can also be a camera or an online colorimeter, etc.

[0062] Continue to refer to Figure 2 As shown, the fluid separation system also includes a first bubble detection element 620, which is located at the outlet or downstream of the centrifuge reservoir 205. The first bubble detection element 620 is used to determine whether there are bubbles in the pipeline, thereby determining the presence or absence of liquid. Optionally, the first bubble detection element 620 is a bubble sensor. In a specific embodiment, the bubble sensor is located on the centrifuge pipeline 116. Further, the bubble sensor can be selected as an ultrasonic bubble sensor or an optical bubble sensor, depending on the requirements.

[0063] Continue to refer to Figure 2 As shown, the fluid separation system also includes a second bubble detector 630, which is installed on the first main pipeline 110. The first main pipeline 110 is connected to the output ports of the buffer solution reservoir 201, the cryopreservation solution reservoir 202, the liquid sample reservoir 203, and the separation solution reservoir 204. The second bubble detector 630 is used to determine whether there are bubbles in the first main pipeline 110, thereby determining whether the liquid in the buffer solution reservoir 201, the cryopreservation solution reservoir 202, the liquid sample reservoir 203, and the separation solution reservoir 204 has been completely discharged. Optionally, the second bubble detector 630 is a bubble sensor. Further, the bubble sensor can be an ultrasonic bubble sensor or an optical bubble sensor, depending on the requirements.

[0064] Continue to refer to Figure 2As shown, in order to accurately control the output of the target object, in some embodiments, the output is weight. The fluid separation system also includes a weighing mechanism 640, on which the target object storage unit 208 is placed. The weighing mechanism 640 is used to obtain the weight of the target object in the target object storage unit 208. Optionally, the weighing mechanism 640 is a weight sensing module, including a hanging rod, on which the target object storage unit 208 is suspended, enabling real-time detection of the actual weight of the target object.

[0065] In some parallel embodiments, the output is a flow rate, and the fluid separation system further includes a flow detection mechanism located at the target inlet 108 and used to acquire the cumulative flow rate of the target entering the target storage container 208. Optionally, the weighing mechanism 640 is a fluid sensor.

[0066] Continue to refer to Figure 2 As shown, the fluid separation system also includes a first pressure detection element 650 and a second pressure detection element 660. The first pressure detection element 650 is used to acquire the pressure at the input end of the power module 400, and the second pressure detection element 660 is used to acquire the pressure at the output end of the power module 400. During the target output operation, the first pressure detection element 650 monitors the pressure within the power pipeline 109 located at the input end of the power module 400. When the negative pressure inside the pipeline falls below a certain value (e.g., -100 mbar), it indicates that the sampling is complete, thereby preventing damage to the target object from rapid changes in negative pressure. Optionally, the first pressure detection element 650 and the second pressure detection element 660 can be pressure sensors, obtaining the pressure inside the pipeline by measuring it outside the pipeline; alternatively, the first pressure detection element 650 and the second pressure detection element 660 can be direct-flow pressure sensors, which obtain the pressure inside the pipeline by directly contacting the liquid.

[0067] It should be noted that the fluid separation system can also perform an airtightness test before performing the pipeline flushing operation. During the airtightness test, the power module 400 and the control valve module 300 operate according to the program control, and based on the pressure values ​​measured from the outside of the pipeline by the first pressure detection element 650 and the second pressure detection element 660, the airtightness of the pipeline consumable module 100 is tested sequentially according to a specific airtightness test method.

[0068] In some embodiments, the power module 400 is a peristaltic pump, which provides the power for infusion. Of course, in addition to a peristaltic pump, the power module 400 may also be other types of pumps.

[0069] Continue to refer to Figure 2As shown, the fluid separation system also includes a rotary joint 710, through which the centrifuge reservoir 205 is rotatably mounted at the centrifuge inlet 105. The rotary joint 710 ensures that the rotation of the centrifuge reservoir 205 does not affect the pipeline, thus preventing the pipeline from becoming tangled.

[0070] Continue to refer to Figure 2 As shown, the fluid separation system also includes a filter 720, which is mounted on the tubing consumable module 100 and located at the buffer inlet 101. The filter 720 ensures that any bacteria that may remain in the buffer solution do not enter the buffer tubing 119. Optionally, the filter 720 is a square filter.

[0071] Continue to refer to Figure 2 As shown, the fluid separation system also includes a puncture device 730, which is located at the buffer solution port 101 and used to puncture the buffer solution reservoir 201. The puncture device 730 makes the assembly of the buffer solution reservoir 201 and the buffer solution port 101 easier.

[0072] Continue to refer to Figure 2 As shown, the fluid separation system also includes a system frame 800 and a display screen 900. The system frame 800 is used to mount a centrifuge module 500, which includes a centrifuge chamber and a squeezing mechanism. The display screen 900 serves as a display and control component, capable of instructing disposable piping consumables to be installed in designated solenoid valves, peristaltic pumps, and sensors on the equipment. The display screen 900 can also run control programs to control the solenoid valves, peristaltic pumps, and sensors to perform their respective functions. Optionally, the display screen 900 is a touchscreen.

[0073] The following describes the working steps of a separation fluid system, with the goal of separating mononuclear cells:

[0074] 1. Piping installation: Install the disposable piping consumable module 100 into the specified solenoid valve, peristaltic pump and sensor on the equipment according to the instructions on the display screen 900, wherein the destination bag is placed on the weighing mechanism 640.

[0075] 2. Air tightness test: The peristaltic pump and solenoid valve of the fluid separation system operate according to the program control. Based on the pressure values ​​measured from the outside of the pipeline by the first pressure detection element 650 and the second pressure detection element 660, the air tightness of the pipeline consumable module 100 is tested sequentially according to a specific air tightness test method. The air tightness test method is existing technology and will not be described in detail here.

[0076] 3. Piping Flushing: Following a specific sequence, a peristaltic pump, in conjunction with solenoid valves, delivers buffer solution from buffer bags to the piping consumable module 100 for flushing. The solenoid valve operation includes a first action and a second action. The first action involves the simultaneous opening of control valves 301, 314, 308, and 310. The second action involves the simultaneous opening of control valves 301, 304, 313, 312, and 310.

[0077] 4. Loading of Separating Liquid: Following a specific timing sequence, a peristaltic pump, in conjunction with a solenoid valve, delivers the separating liquid from the separation bag to the centrifuge bag. Once the second bubble detector 630 detects bubbles, it indicates that loading of the separating liquid is complete. Simultaneously, the centrifuge chamber rotates at a specified speed, causing the centrifuge bag to rotate. The operation of the solenoid valves includes the simultaneous opening of the sixth control valve 306, the thirteenth control valve 313, and the eleventh control valve 311.

[0078] 5. Sample Loading: Control valves 5 (305), 13 (313), and 11 (311) open, and the peristaltic pump delivers the liquid sample from the sample bag to the centrifuge bag at a certain flow rate. Once a certain amount of sample has been loaded, or the second bubble detector 630 detects that the sample loading in the sample bag is complete, all solenoid valves close. Subsequently, the peristaltic pump, in conjunction with the solenoid valves, flushes the sample bag with the buffer solution from the buffer bag and delivers it back to the centrifuge bag to ensure the sample is completely loaded. Simultaneously, the centrifuge chamber rotates at a specified speed, causing the centrifuge bag to rotate. Under centrifugal force, the separation liquid and liquid sample in the centrifuge bag separate along the radius of the centrifuge bag. During this separation process, red blood cells pass through the separation liquid layer to the outermost layer of the centrifuge bag, while the mononuclear cell layer is separated from the red blood cell layer by the separation liquid. After the liquid sample in the centrifuge bag has been centrifuged for the specified time, the 11th control valve 311 is notified. 11. The twelfth control valve 312, the eighth control valve 308, and the seventh control valve 307 are opened. A specific squeezing mechanism in the centrifuge chamber rises and squeezes the centrifuge bag. The rotary joint 710 ensures that while the centrifuge bag is rotating, the liquid inside can be squeezed into the relatively stationary pipeline, thereby discharging the intermediate into the intermediate storage container 206. The color sensor emitter emits infrared light of a certain intensity. When it detects the red blood cell layer in the pipeline, the color sensor infrared receiver receives a light intensity change signal. When this signal reaches a set threshold, it will stop the squeezing mechanism in the centrifuge chamber from rising, or the centrifuge chamber squeezing mechanism will stop rising after rising to a certain height and control the aforementioned solenoid valve to close, so as to prevent red blood cells from entering the intermediate bag. Finally, the peristaltic pump, in conjunction with the solenoid valve, delivers buffer solution to flush the residual waste liquid in the pipeline into the waste liquid bag. The cleaning operation of this step is the same as the pipeline rinsing described above.

[0079] 6. Centrifuge storage unit cleaning: The buffer solution in the buffer bag is output to the centrifuge bag by the action of the peristaltic pump and the solenoid valve. The solenoid valve is activated by opening the first control valve 301, the fourth control valve 304, the thirteenth control valve 313 and the eleventh control valve 311 at the same time. Then the liquid in the centrifuge bag is discharged into the waste liquid bag. This operation is repeated to clean the centrifuge bag 4 times.

[0080] 7. Intermediate loading: The intermediate material in the intermediate storage unit 206 is loaded into the centrifuge bag by the action of the peristaltic pump and the solenoid valve. The solenoid valve is activated so that the seventh control valve 307, the thirteenth control valve 313 and the eleventh control valve 311 are opened at the same time.

[0081] 8. Sample Cleaning: Control valves 301, 304, 313, and 311 open, and the peristaltic pump delivers a certain amount of buffer solution to the centrifuge bag at a certain flow rate. Simultaneously, a specific squeezing mechanism in the centrifuge chamber descends. After the buffer solution is loaded, the peristaltic pump stops and the solenoid valve closes. The centrifuge chamber rotates the centrifuge bag at a specified speed for a certain period of time. Under the action of centrifugal force, the blood sample in the centrifuge bag will form different layers along the radius of the centrifuge bag. After the specified centrifugation time is reached, the centrifuge chamber decelerates to the specified speed. Then, control valves 311, 312, and 310 open, and the specific squeezing mechanism in the centrifuge chamber rises to squeeze the centrifuge bag, discharging the waste liquid after centrifugation into the waste liquid bag. After the centrifuge chamber squeezes the centrifuge bag to the set height, the peristaltic pump, in conjunction with the solenoid valve, cleans the corresponding pipelines.

[0082] 9. Replace the cryopreservation solution. The third control valve 303, the fourth control valve 304, the thirteenth control valve 313 and the eleventh control valve 311 are opened. The peristaltic pump delivers a certain amount of cryopreservation solution from the cryopreservation solution bag to the centrifuge bag at a certain flow rate. Then, the sample in the centrifuge bag is centrifuged in the same way and the waste liquid is discharged. Finally, the centrifuge bag contains the set liquid volume, for example, 20 ml.

[0083] 10. Output of Target Cells: After the cryopreservation solution is replaced, the centrifuge chamber rotates in both directions at a certain speed to mix the cells around the centrifuge bag and allow them to flow to the center of the centrifuge bag. Then, control valves 11 (311), 13 (313), 8 (308), and 9 (309) are opened. The peristaltic pump draws the target cells from the centrifuge bag to the target bag at a certain flow rate. To ensure the cell viability of the final product, the second pressure sensor 660 monitors the pressure inside the tube at the inlet of the peristaltic pump. When the negative pressure inside the tube falls below a certain value (e.g., -100 mbar), the sampling is complete, thus preventing damage to the final product cells from rapid changes in negative pressure (pressure change reference). Figure 3 The two series represent data from two separate tests.

[0084] The above-described separation fluid system and method for separating mononuclear cells are applicable to the separation of mononuclear cells from peripheral blood, umbilical cord blood, and tissue samples. Taking the separation of mononuclear cells from peripheral blood as an application example, following the above steps, the peristaltic pump, solenoid valve, and tubing consumable module 100 in the separation fluid system complete the following steps in a predetermined sequence: tubing installation, airtightness testing, tubing rinsing, loading of the separation fluid, sample loading and volume adjustment, cleaning of centrifuge storage containers, loading of intermediates, sample cleaning and replacement of cryopreservation solution, and output of the target substance. This completes the separation of mononuclear cells from the sample, achieving a single-cell recovery rate that meets storage requirements, and the final product volume remains stable at 40 ml.

[0085] The aforementioned fluid separation system and method for mononuclear cells has the following advantages: 1. It can efficiently separate samples, achieving fully automated mononuclear cell separation. During the output process, the intermediate output is controlled by a color detection threshold to detect the erythrocyte layer, preventing erythrocytes from entering the intermediate bag and ensuring the purity of mononuclear cells in the intermediate sample. During the output of the target material, a pressure monitoring strategy controls the negative pressure change at the inlet of the peristaltic pump, thereby preventing excessive negative pressure from damaging the cells, reducing damage to the target cells during cell separation, and ensuring good cell viability in the final product. 2. The final product output volume is precisely controlled by the mononuclear cell separation sequence combined with a weighing strategy, facilitating subsequent reagent addition and cryopreservation operations. 3. Through standardized automatic control methods, combined with the tubing consumable module 100, various complex operations are completed, effectively avoiding errors and contamination risks that may occur during manual operation, greatly improving experimental efficiency, and ensuring the reproducibility of GMP-grade cell preparation. 4. By establishing cell storage services in different regions, we can directly provide self-developed automated instruments and continuously supply tubing consumable modules 100, which benefits cell storage customers and brings continuous economic benefits.

[0086] The technical problems solved by the fluid separation system provided by this utility model are as follows: 1. By automating the process and using low-cost disposable closed-loop consumables, the mononuclear cell separation process is completed, resulting in a fixed volume of final product, reducing reliance on manual operation. 2. The system timing and weighing strategy are used to precisely control the output volume of the final product, solving the problem of poor control over the output volume. 3. A color sensor is used to detect the liquid color change in real time during intermediate sample dispensing, thereby reducing the output of red blood cells into the intermediate bag during the mononuclear cell dispensing process, ensuring the purity of mononuclear cells in the intermediate bag, and solving the problem of insufficient purity of mononuclear cells in the final product. 4. A pressure monitoring strategy is used to control the pressure changes during the pumping of the final product, preventing excessive negative pressure from damaging the cells, ensuring good cell viability in the final product, and solving the problem of cell damage caused by excessive negative pressure.

[0087] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating the present utility model, and are not intended to limit the implementation of the present utility model. Those skilled in the art can make various obvious changes, readjustments, and substitutions without departing from the protection scope of this utility model. It is neither necessary nor possible to exhaustively describe all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.

Claims

1. A fluid separation system, characterized in that, include: The tubing consumable module (100) has multiple ports, including a buffer solution port (101), a cryopreservation solution port (102), a liquid sample port (103), a separation solution port (104), a centrifuge port (105), an intermediate product port (106), a waste liquid port (107), and a destination product port (108). The liquid storage module (200) has multiple storage components, including a buffer solution storage component (201), a cryopreservation solution storage component (202), a liquid sample storage component (203), a separation solution storage component (204), a centrifuge sample storage component (205), an intermediate product storage component (206), a waste liquid storage component (207), and a target product storage component (208), which are installed one-to-one on the buffer solution port (101), the cryopreservation solution port (102), the liquid sample port (103), the separation solution port (104), the centrifuge port (105), the intermediate product port (106), the waste liquid port (107), and the target product port (108); A control valve module (300) is provided on the pipeline consumable module (100) and is capable of selectively controlling the connection of at least two of the storage components; A power module (400) is provided on the pipeline consumable module (100) and is used to provide power for the flow of liquid between the storage components; A centrifuge module (500) is provided at the centrifuge storage container (205) and is used to centrifuge and mix the liquid in the centrifuge storage container (205); The separation fluid system is configured to allow the liquid in the storage container to flow according to a preset time sequence and preset path to perform pipeline rinsing, separation liquid loading, sample loading, centrifuge storage container cleaning, intermediate loading, sample cleaning, cryopreservation liquid replacement, and target material output operations, so as to separate the target material from the liquid sample and store it in the target material storage container (208).

2. The fluid separation system according to claim 1, characterized in that, The fluid separation system also includes a color detection mechanism (610), which is located on the centrifugal pipeline (116) and is used to obtain the color of the liquid discharged from the centrifugal storage container (205). The end of the centrifugal pipeline (116) is connected to the outlet of the centrifugal storage container (205).

3. The fluid separation system according to claim 1, characterized in that, The fluid separation system further includes a weighing mechanism (640), on which the target material storage container (208) is placed, and the weighing mechanism (640) is used to obtain the weight of the target material in the target material storage container (208); Alternatively, the fluid separation system may further include a flow detection mechanism located at the destination inlet (108) and used to obtain the cumulative flow of the destination entering the destination storage container (208).

4. The fluid separation system according to claim 1, characterized in that, The separation fluid system further comprises a first pressure detection member (650) and a second pressure detection member (660), wherein the first pressure detection member (650) is configured to obtain a pressure at an input end of the power module (400), and the second pressure detection member (660) is configured to obtain a pressure at an output end of the power module (400).

5. The fluid separation system according to claim 1, characterized in that, The separation fluid system further comprises a rotary joint (710), and the centrifugate storage member (205) is rotatably mounted at the centrifugal nozzle (105) through the rotary joint (710); and / or, the separation fluid system further comprises a filter (720), wherein the filter (720) is configured to filter buffer flowing out of the buffer storage member (201); and / or, the separation fluid system further comprises a puncture device (730), wherein the puncture device (730) is configured to puncture into the buffer storage member (201).

6. The fluid separation system according to any one of claims 1-5, characterized in that, The pipeline consumable module (100) comprises a power pipeline (109) and an annular pipeline which are communicated in a shape of a Chinese character "ri", the power module (400) is arranged on the power pipeline (109), and all the storage members are communicated with the annular pipeline.

7. The fluid separation system according to claim 6, characterized in that, The annular pipeline comprises a first main pipeline (110), a second main pipeline (111), a third main pipeline (112), a fourth main pipeline (113), a fifth main pipeline (114) and a sixth main pipeline (115) which are communicated end to end, the second main pipeline (111) and the third main pipeline (112) have a first communication point, the third main pipeline (112) and the fourth main pipeline (113) have a second communication point, the fifth main pipeline (114) and the sixth main pipeline (115) have a third communication point, and the sixth main pipeline (115) and the first main pipeline (110) have a fourth communication point; The pipeline consumable module (100) further includes a centrifuge pipeline (116), a waste discharge pipeline (117), a target material pipeline (118), a buffer pipeline (119), a cryopreservation pipeline (120), a sample pipeline (121), a separation pipeline (122), and an intermediate material pipeline (123). One end of the centrifuge pipeline (116) is connected to the second connection point, and the other end forms the centrifuge port (105). One end of the waste discharge pipeline (117) is connected to the third main pipeline (112), and the other end forms the waste liquid port (107). One end of the target material pipeline (118) is connected to the first connection point, and the other end forms the target material port (108). One end of the buffer line (119) is connected to the third connection point, and the other end forms the buffer solution port (101). One end of the cryopreservation line (120) is connected to the sixth main line (115), and the other end forms the cryopreservation liquid port (102). One end of the sample line (121) is connected to the sixth main line (115), and the other end forms the liquid sample port (103). One end of the separation line (122) is connected to the sixth main line (115), and the other end forms the separation liquid port (104). One end of the intermediate material line (123) is connected to the fourth connection point, and the other end forms the intermediate material port (106). And / or, the pipeline consumable module (100) further includes a spare pipeline (124), one end of which is connected to the sixth main pipeline (115), and the other end forms a spare port (125).

8. The fluid separation system according to claim 7, characterized in that, The control valve module (300) includes a first control valve (301) on the buffer line (119), a second control valve (302) on the backup line (124), a third control valve (303) on the cryopreservation line (120), a fourth control valve (304) on the sixth main line (115), a fifth control valve (305) on the sample line (121), a sixth control valve (306) on the separation line (122), and a seventh control valve (307) on the intermediate material line (123). The following valves are provided: the eighth control valve (308) on the second main pipeline (111), the ninth control valve (309) on the destination pipeline (118), the tenth control valve (310) on the waste discharge pipeline (117), the eleventh control valve (311) on the centrifuge pipeline (116), the twelfth control valve (312) on the third main pipeline (112), the thirteenth control valve (313) on the fourth main pipeline (113), and the fourteenth control valve (314) on the fifth main pipeline (114).

9. The fluid separation system according to any one of claims 1-5, characterized in that, The fluid separation system has a first waste discharge channel, which includes a centrifugal pipeline (116), a portion of a third main pipeline (112), and a waste discharge pipeline (117) connected in sequence. The centrifugal pipeline (116) has a centrifugal port (105) at the end away from the third main pipeline (112), and the waste discharge pipeline (117) has a waste liquid port (107) at the end away from the third main pipeline (112). The centrifugal pipeline (116) is equipped with an eleventh control valve (311), the third main pipeline (112) is equipped with a twelfth control valve (312), and the waste discharge pipeline (117) is equipped with a tenth control valve (310).

10. The fluid separation system according to any one of claims 1-5, characterized in that, The fluid separation system has a second waste discharge channel, which includes a centrifugal pipeline (116), a fourth main pipeline (113), a power pipeline (109), a second main pipeline (111), a portion of a third main pipeline (112), and a waste discharge pipeline (117) connected in sequence. The centrifugal pipeline (116) has a centrifugal port (105) at the end away from the fourth main pipeline (113), and the waste discharge pipeline (117) has a waste liquid port (107) at the end away from the third main pipeline (112). The power module (400) is located on the power pipeline (109). The centrifugal pipeline (116) is equipped with an eleventh control valve (311), the fourth main pipeline (113) is equipped with a thirteenth control valve (313), the second main pipeline (111) is equipped with an eighth control valve (308), and the waste discharge pipeline (117) is equipped with a tenth control valve (310).

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