A fermentation seed tank aseptic maintenance system

Abstract

The utility model discloses a kind of fermentation seed tank aseptic maintenance system, belong to fermentation microbial culture technical field, this aseptic maintenance system includes two filters in parallel with seed tank, two filters are filtered alternately with seed tank communication to the air of entering seed tank, two filters have parallel steam cleaning assembly, when any one filter is communicated with seed tank, another filter is communicated with steam cleaning assembly and carries out steam cleaning;It also includes flask and inoculation bottle, flask is used for spore germination and part of bacterial body growth stage, to reduce seed tank running time.The utility model when one filter works, another can carry out cleaning disinfection and maintenance simultaneously, ensure the absolute continuity of air supply, and ensure the quality of air of entering seed tank, reduce the probability of contamination, simultaneously part of microbial process spore germination and initial growth are transferred from seed tank to flask and carry out, greatly shorten the running time in seed tank.

Claims

1. A sterile maintenance system for a fermentation seed tank, characterized in that, It includes two filters connected in parallel with the seed tank. The two filters are alternately connected to the seed tank to filter the air entering the seed tank. The two filters are connected in parallel with a steam cleaning assembly, which can perform steam cleaning on the filters. When either filter is connected to the seed tank, the other filter is connected to the steam cleaning assembly for steam cleaning. It also includes shake flasks and inoculation bottles. Shake flasks are used for spore germination and part of the cell growth stage. The shake flasks can be mechanically shaken back and forth. After the shake flask culture is completed, the fermentation broth from the shake flasks is collected into the inoculation bottle. The inoculation bottle transfers the fermentation broth into the seed tank under aseptic conditions to reduce the seed tank running time.

2. The aseptic maintenance system for the fermentation seed tank according to claim 1, characterized in that, The two filters are the first filter and the second filter, respectively; When the first filter is connected to the seed tank, the second filter is not connected to the seed tank, and the first filter is not connected to the steam cleaning assembly, while the second filter is connected to the steam cleaning assembly. When the second filter is connected to the seed tank, the first filter is not connected to the seed tank, and the second filter is not connected to the steam cleaning assembly, while the first filter is connected to the steam cleaning assembly.

3. The aseptic maintenance system for the fermentation seed tank according to claim 2, characterized in that, It also includes a three-way valve, which has a first inlet, a second inlet and a first outlet. The three-way valve is connected to the outlet end of the first filter through the first inlet, the three-way valve is connected to the outlet end of the second filter through the second inlet, and the three-way valve is connected to the seed tank through the first outlet. The three-way valve has a first state and a second state. When the three-way valve is in the first state, the outlet of the first filter is connected to the seed tank through the three-way valve, and the second filter is connected to the steam cleaning assembly for steam cleaning. When the three-way valve is in the second state, the outlet of the second filter is connected to the seed tank through the three-way valve, and the first filter is connected to the steam cleaning assembly for steam cleaning.

4. The aseptic maintenance system for the fermentation seed tank according to claim 3, characterized in that, The inlet end of the first filter is connected to a first inlet valve, and the inlet end of the second filter is connected to a second inlet valve. The first inlet valve and the second inlet valve are opened and closed alternately. When the first inlet valve is open, the three-way valve is in the first state, which connects the first filter to the seed tank to filter the air entering the seed tank; When the second inlet valve is open, the three-way valve is in the second state, which connects the second filter to the seed tank to filter the air entering the seed tank.

5. The aseptic maintenance system for the fermentation seed tank according to claim 2, characterized in that, The steam cleaning assembly includes a steam tank, a first inlet valve, and a second inlet valve. The steam tank is connected to the inlet end of the first filter via the first inlet valve, and the steam tank is connected to the inlet end of the second filter via the second inlet valve. When the three-way valve is in the second state, the first inlet valve is activated, the steam tank is connected to the first filter, and the exhaust end of the first filter is closed. The steam tank performs steam cleaning on the first filter. When the three-way valve is in the first state, the second air inlet valve is activated, the steam tank is connected to the second filter, and the exhaust end of the second filter is closed. The steam tank then performs steam cleaning on the second filter.

6. The aseptic maintenance system for the fermentation seed tank according to claim 5, characterized in that, The steam cleaning assembly also includes a first exhaust valve and a second exhaust valve, the first exhaust valve being connected to the exhaust end of the first filter and the second exhaust valve being connected to the exhaust end of the second filter; When the three-way valve is in the second state and the steam tank has finished steam cleaning the first filter, the first inlet valve is closed and the first exhaust valve is opened to discharge the steam in the first filter. With the three-way valve in the first position and the steam tank having completed steam cleaning of the second filter, the second inlet valve closes and the second exhaust valve opens to discharge the steam from the second filter.

7. The aseptic maintenance system for the fermentation seed tank according to claim 1, characterized in that, The filter is connected to an air purge valve, through which sterile air can be injected; After the steam tank has finished steam cleaning the filter, when the steam is discharged from the filter, open the air purge valve and inject sterile air to dry the filter.

8. The aseptic maintenance system for the fermentation seed tank according to claim 1, characterized in that, The filter is equipped with a drain valve, which can drain the liquid accumulated in the filter. When any one filter is connected to the seed tank, the other filter first opens its drain valve to drain the accumulated liquid. After the liquid is drained, the drain valve closes, and the other filter is connected to the steam cleaning assembly for steam cleaning.

9. The aseptic maintenance system for the fermentation seed tank according to claim 1, characterized in that, The filter is equipped with a drain valve, which can drain condensate. When the steam tank is used to steam clean the filter, the steam trap automatically opens and closes to drain the condensate and maintain the dryness of the steam.

10. The aseptic maintenance system for the fermentation seed tank according to claim 1, characterized in that, The filter is equipped with a safety valve. When the filter is steam-cleaned by the steam tank and the steam pressure inside the filter exceeds the limit, the safety valve will automatically release the steam.

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