Electrophoresis chip and analysis device

Abstract

The application discloses an electrophoresis chip and an analysis device. The electrophoresis chip comprises a substrate and a cover plate covering the substrate; a plurality of flow channels for containing samples to be measured are arranged on the substrate, a sample adding groove is arranged at a sample adding position of each flow channel, and the extension direction of the sample adding groove is the same as the extension direction of the corresponding flow channel; electrode assemblies are arranged at both ends of each flow channel, and the electrode contact end of each flow channel and the corresponding electrode assembly is arranged in a staggered mode, so that the extension direction of the sample adding groove is not intersected with the vertical center line direction of the electrode contact end. The electrode contact end of the flow channel and the corresponding electrode assembly is arranged in a staggered mode, and the extension direction of the sample adding groove is not intersected with the vertical center line direction of the electrode contact end, so that spatial interference is eliminated, parallel execution of sample adding operation and electrophoresis operation is realized, and the detection efficiency of the electrophoresis chip is improved.

Description

Technical Field

[0001] This application relates to the field of biological detection technology, specifically to an electrophoresis chip and analysis device. Background Technology

[0002] Electrophoretic separation technology, due to its high resolution and high sensitivity, has become a fundamental method in fields such as biochemical analysis, drug screening, and gene detection. Electrophoresis chips, by arranging multiple microchannels in parallel on the same substrate, enable simultaneous detection of multiple samples and have become a core tool for high-throughput biochemical analysis. However, traditional electrophoresis chips typically place the electrode contacts at the ends of the channels, with the sample loading orifice too close to the electrode contacts. This causes the sample loading needle, puncture needle, and horizontally inserted electrophoresis probe to overlap in space, requiring time-sharing operation. This means that sample loading and electrophoresis are performed sequentially, resulting in low detection efficiency. Utility Model Content

[0003] This application provides an electrophoresis chip and analysis device. By misaligning the flow channel with the electrode contact ends of the corresponding electrode assembly and ensuring that the extension direction of the sample loading groove does not intersect with the vertical center line direction of the electrode contact ends, spatial interference is eliminated, and the parallel execution of the sample loading operation and electrophoresis operation is achieved, thereby improving the detection efficiency of the electrophoresis chip.

[0004] This application provides an electrophoresis chip, including a substrate and a cover plate covering the substrate;

[0005] The substrate is provided with multiple flow channels for accommodating the sample to be tested. Each flow channel has a sample dispensing slot at the sample dispensing position. The extension direction of the sample dispensing slot is the same as the extension direction of the corresponding flow channel.

[0006] Each flow channel is provided with electrode assemblies at both ends, and the electrode contact ends of each flow channel and the corresponding electrode assembly are staggered so that the extension direction of the sample loading groove does not intersect with the vertical center line direction of the electrode contact end.

[0007] This application also provides an analysis apparatus, including:

[0008] The chip fixing mechanism is mounted on the base plate;

[0009] A sample dispensing mechanism is used to sequentially dispense samples into each channel of the flow channel;

[0010] An electrophoresis apparatus, mounted on a base plate, is used to perform electrophoresis on the flow channel after sample loading; and

[0011] An imaging mechanism, mounted on the base plate, is used to photograph each flow channel after the electrophoresis operation has been completed.

[0012] The electrophoresis chip provided in this application includes a substrate and a cover plate covering the substrate. The substrate has multiple channels for accommodating samples, and each channel has a sample loading position with a loading groove whose extension direction is the same as that of the corresponding channel. Each channel has electrode assemblies at both ends, and the electrode contact ends of each channel and the corresponding electrode assembly are offset, ensuring that the extension direction of the loading groove does not intersect with the vertical centerline direction of the electrode contact end. By offsetting the channels with the electrode contact ends of the corresponding electrode assemblies and ensuring that the extension direction of the loading groove does not intersect with the vertical centerline direction of the electrode contact end, this application eliminates spatial interference, enables parallel execution of the sample loading and electrophoresis operations, and improves the detection efficiency of the electrophoresis chip. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below.

[0014] Figure 1 This is a schematic diagram of the analysis apparatus provided in an embodiment of this application.

[0015] Figure 2 The embodiments provided in this application are based on Figure 1 A magnified view of part A;

[0016] Figure 3 A schematic diagram of the fixing plate provided in an embodiment of this application;

[0017] Figure 4 A schematic diagram of the electrophoresis fixation plate provided in an embodiment of this application;

[0018] Figure 5 A schematic diagram of the imaging mechanism provided in the embodiments of this application;

[0019] Figure 6 A schematic diagram from another perspective of the analysis apparatus provided in an embodiment of this application;

[0020] Figure 7 The embodiments provided in this application are based on Figure 6 A magnified view of part B;

[0021] Figure 8 A schematic diagram of the baffle provided in an embodiment of this application;

[0022] Figure 9 A schematic diagram illustrating the movement of the pusher and baffle provided in an embodiment of this application;

[0023] Figure 10 A schematic diagram of the sample addition mechanism provided in the embodiments of this application;

[0024] Figure 11The implementation method provided in this application is based on Figure 10 A magnified view of part C;

[0025] Figure 12 A schematic diagram of an electrophoretic chip substrate provided in an embodiment of this application;

[0026] Figure 13 A schematic diagram of the structure of a first type of printed electrode layer projected orthogonally onto the first surface of an electrophoretic chip substrate provided in an embodiment of this application;

[0027] Figure 14 A schematic diagram of the structure of the second type of printed electrode layer projected onto the first surface of the electrophoretic chip substrate provided in the embodiments of this application;

[0028] Figure 15 This is a schematic diagram of the second surface of the electrophoretic chip substrate provided in an embodiment of this application;

[0029] Figure 16 A schematic diagram of the structure of the electrophoretic chip cover plate provided in the embodiments of this application, wherein a first type of printed electrode layer is disposed on the fourth surface;

[0030] Figure 17 A schematic diagram of a structure in which a second type of printed electrode layer is disposed on the fourth surface of an electrophoretic chip cover plate provided in an embodiment of this application;

[0031] Figure 18 This is a schematic diagram of the third surface of the electrophoretic chip cover plate provided in an embodiment of this application. Detailed Implementation

[0032] The embodiments of this application are described in detail below, with examples of these embodiments illustrated in the accompanying drawings. Obviously, the described embodiments are merely some, not all, of the embodiments of this application. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this application, and should not be construed as limiting this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0033] In the description of this application, it should be understood that the terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," and "counterclockwise," etc., indicating orientation or positional relationships based on the orientation or positional relationships shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, features defined with "first" and "second" may explicitly or implicitly include one or more features. In the description of this application, "multiple" means two or more, unless otherwise explicitly specified.

[0034] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between them; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication between two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0035] In this application, unless otherwise expressly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature being directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature being directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0036] The following disclosure provides many different embodiments or examples for implementing different structures of this application. To simplify the disclosure, specific examples of components and arrangements are described below. Of course, these are merely examples and are not intended to limit the scope of this application. Furthermore, reference numerals and / or letters may be repeated in different examples; such repetition is for simplification and clarity and does not in itself indicate a relationship between the various embodiments and / or arrangements discussed. In addition, various specific examples of processes and materials are provided in this application, but those skilled in the art will recognize the application of other processes and / or the use of other materials.

[0037] The following sections provide detailed descriptions of each example. It should be noted that the order in which the embodiments are described is not intended to limit the priority of the embodiments.

[0038] Please see as follows Figures 1 to 11 This application provides an analysis device 10, which includes:

[0039] The base plate 100 has a first cavity 110.

[0040] The chip fixing mechanism 200 is disposed on the base plate 100 and includes at least one analysis station located in the first cavity 110. The analysis station is used to vertically place the electrophoresis chip 2000, which has multiple channels 2140 for accommodating the sample to be tested.

[0041] The sample loading mechanism 600 is used to sequentially load samples into each of the multiple channels 2140 in the electrophoresis chip 2000.

[0042] An electrophoresis apparatus 300, mounted on a base plate 100, is used to perform electrophoresis on the flow channel after sample loading, so that the samples to be tested within the flow channel 2140 are separated by electrophoresis; and

[0043] An imaging mechanism 400, mounted on a base plate 100, is used to take pictures of each channel 2140 after the electrophoresis operation 2140 has been completed, so as to obtain a sample imaging image corresponding to each channel 2140.

[0044] In some embodiments, the analysis device 10 further includes a blocking mechanism 500 disposed in the first cavity 110 and corresponding to the location of the analysis station, including at least one recycling station.

[0045] In this embodiment, when the electrophoresis chip 2000 is located at the analysis station, the chip fixing mechanism 200 fixes the electrophoresis chip 2000, and the blocking mechanism 500 prevents the electrophoresis chip 2000 from falling. The sample loading mechanism 600 sequentially loads samples into each of the multiple channels 2140 within the electrophoresis chip 2000. The electrophoresis mechanism 300 performs electrophoresis on the channels 2140 within the electrophoresis chip 2000 where the sample loading operation has been completed, so that the samples to be tested within the channels 2140 are separated by electrophoresis. The imaging mechanism 400 takes pictures of each channel that has completed the electrophoresis operation to obtain an image of the sample corresponding to each channel. Finally, the chip fixing mechanism 200 releases the electrophoresis chip 2000, and the blocking mechanism 500 moves, causing the electrophoresis chip 2000 to fall to the recycling station.

[0046] The base plate 100 can be designed as a square structure, and the chip fixing mechanism 200, electrophoresis mechanism 300, imaging mechanism 400 and blocking mechanism 500 are disposed on the base plate 100. Multiple support feet are provided on the lower surface of the base plate 100 to provide support for the base plate 100.

[0047] In this embodiment, the electrophoresis chip 2000 can be directly placed at the analysis station of the chip fixing mechanism 200, or it can be placed at the entrance of the analysis device 10. A moving mechanism, such as a robotic arm or conveyor belt, can then move the electrophoresis chip 2000 from the entrance to the analysis station of the chip fixing mechanism 200. When the electrophoresis chip 2000 is at the analysis station, the chip fixing mechanism 200 secures it, preventing movement during subsequent electrophoretic separation and imaging. At this time, the blocking mechanism 500 is in its initial position, preventing the electrophoresis chip 2000 from falling. After the chip fixing mechanism 200 fixes the electrophoresis chip 2000, the sample loading mechanism 600 sequentially loads samples into each of the multiple channels 2140 within the electrophoresis chip 2000. The electrophoresis mechanism 300 performs electrophoresis on the channels 2140 within the electrophoresis chip 2000 where samples have been loaded, enabling the samples to be tested within the channels 2140 to undergo electrophoretic separation. The imaging mechanism 400 takes a picture of each channel 2140 that has completed the electrophoresis operation to obtain an image of the sample corresponding to each channel 2140. The obtained image images are then analyzed to determine information such as the fragment length, concentration, and integrity of the sample. After the analysis is completed, the chip fixing mechanism 200 releases the electrophoresis chip 2000, no longer fixing it, allowing the electrophoresis chip 2000 to move. The blocking mechanism 500 moves from its initial position, and the electrophoresis chip 2000 falls to the recovery station after losing the blocking effect of the blocking mechanism 500. After completing the analysis and recycling of one electrophoresis chip 2000, the same operation is performed on the next electrophoresis chip 2000 until the analysis and recycling of all electrophoresis chips 2000 are completed.

[0048] The analytical apparatus 10 provided in this application can perform electrophoretic analysis on a sample containing biological substances such as DNA, RNA, or proteins using electrophoresis technology. In this document, "sample" and "sample to be analyzed" refer to the same thing: the sample loaded into the electrophoresis chip 2000 for analysis.

[0049] For example, taking a nucleic acid sample containing DNA or RNA as the test sample, when the electrophoresis chip 2000 is in the analysis station, after the chip fixing mechanism 200 fixes the electrophoresis chip 2000, the sample loading mechanism 600 sequentially loads samples into each of the multiple channels 2140 in the electrophoresis chip 2000. The electrophoresis mechanism 300 performs electrophoresis on the channels 2140 in the electrophoresis chip 2000 where the sample loading operation has been completed, so that the test samples in the channels 2140 are separated by electrophoresis. That is, the electrophoresis mechanism 300 applies a voltage to the nucleic acid samples in the channels 2140 in the electrophoresis chip 2000 where the sample loading operation has been completed. The nucleic acid samples move under the action of the electric field force. Since there are nucleic acid fragments of different lengths in the nucleic acid samples, differential separation of nucleic acid fragments of different lengths is achieved, forming multiple bands. After the nucleic acid samples undergo electrophoretic separation, the imaging mechanism 400 photographs each channel 2140 that has completed the electrophoresis operation to obtain a sample imaging image corresponding to each channel 2140. The photographed sample imaging images are then analyzed to determine information such as the length, concentration, and integrity of the nucleic acid fragments corresponding to each band in the nucleic acid sample. After the analysis is complete, the chip fixing mechanism 200 releases the electrophoresis chip 2000, the blocking mechanism 500 moves, and the electrophoresis chip 2000 falls to the recycling station. For samples containing other biological materials, the same process can be performed: sample addition by the sample addition mechanism 600, electrophoresis separation by the electrophoresis mechanism 300, and imaging by the imaging mechanism 400. Finally, the photographed sample imaging images are analyzed to complete the analysis process.

[0050] Furthermore, a molecular weight standard (ladder) is set up. The nucleic acid sample and the molecular weight standard undergo electrophoretic separation under the influence of an electric field. In subsequent analysis based on the obtained images, the standard reference band separated by the molecular weight standard is used as a benchmark. The information of the standard reference band separated by the molecular weight standard and the information of the separated nucleic acid sample (i.e., the brightness and position information of the band objects in the sample imaging image) are compared to calculate the band information in the nucleic acid sample (test sample) corresponding to each flow channel 2140. This band information includes the corresponding nucleic acid fragment length, concentration, and nucleic acid integrity index. Of course, it is possible to calculate the nucleic acid fragment length, concentration, and nucleic acid integrity index corresponding to the separated band objects directly from the information of the nucleic acid sample after electrophoretic separation without setting up a molecular weight standard.

[0051] In this embodiment, as Figure 1 , Figure 6 , Figure 7 , Figure 8 , Figure 9The blocking mechanism 500 includes: a baffle 520 disposed in the first cavity 110 and corresponding to the position of the analysis station; and a first driving member 510 drivenly connected to the baffle 520.

[0052] The first driving component 510 can drive the baffle 520 to move. If the first driving component 510 is a motor, the baffle 520 is provided with a protrusion 522, and the output shaft of the motor is connected to the protrusion 522. When the motor is working, it drives the protrusion 522 to move through the output shaft, thereby driving the baffle 520 to move. To save space, the first driving component 510 is located below the base plate 100.

[0053] The baffle 520 is designed with a square structure. Whether the electrophoresis chip 2000 is placed vertically or horizontally at the analysis station, the baffle 520 can prevent the electrophoresis chip 2000 from falling. After the baffle 520 moves, it loses its blocking effect and the electrophoresis chip 2000 can fall to the recycling station. The baffle 520 is located in the first cavity 110, and the horizontal surface of the baffle 520 is flush with the first cavity 110. This ensures that the electrophoresis chip 2000 can move smoothly without unevenness in the contact surface.

[0054] When the electrophoresis chip 2000 is placed in the analysis station, the baffle 520 is in its initial position, which can block the electrophoresis chip 2000 and prevent it from falling. After the electrophoresis chip 2000 has completed the analysis, the first driving unit 510 drives the baffle 520 to move, and the electrophoresis chip 2000 loses the blocking effect of the baffle 520 and falls to the recycling station.

[0055] In some specific embodiments of this application, reference is made to Figure 1 and Figure 6 The blocking mechanism 500 also includes a bending plate 530 with a certain bending angle disposed in the first cavity 110 and corresponding to the location of the analysis station. When the electrophoresis chip 2000 falls, it falls along the bending plate 530 to the recycling station.

[0056] The bending plate 530 has through holes 211 on both sides. Screws passing through the through holes 211 allow the bending plate 530 to be fixedly connected to the inner wall of the first cavity 110. The bending plate 530 is made of a material that is not easily deformed, such as iron, steel, or aluminum alloy. The bending plate 530 includes a first surface and a second surface, forming a certain bending angle between the first surface and the second surface, such as 50°, 60°, 70°, or other angles. After the sample to be tested in the electrophoresis chip 2000 has been analyzed, the first driving component 510 drives the baffle 520 to move. The electrophoresis chip 2000 loses the obstruction of the baffle 520 and begins to fall. The electrophoresis chip 2000 first falls onto the first surface, then along the first and second surfaces, and falls to the recycling station, or first falls onto the second surface, and then along the second surface, and falls to the recycling station.

[0057] The bending plate 530 provides a certain guiding function to prevent the electrophoresis chip 2000 from falling smoothly into the recycling station. Furthermore, the bending plate 530 provides a certain cushioning function to prevent damage to the electrophoresis chip 2000 and leakage of the test sample inside the electrophoresis chip 2000 when it falls from top to bottom into the recycling station.

[0058] In some specific embodiments of this application, the recycling station is equipped with a waste wafer bin. After the sample to be analyzed within the electrophoresis chip 2000 is completed, the blocking mechanism 500 moves, and the electrophoresis chip 2000 falls into the waste wafer bin of the recycling station. After all electrophoresis chips 2000 have been analyzed or after a certain period of time, the user can recycle the analyzed electrophoresis chips 2000 from the waste wafer bin.

[0059] In this embodiment, as Figures 1 to 3 As shown, the chip fixing mechanism 200 includes a fixing plate 210 and a pushing mechanism that provides a pushing force to the electrophoretic chip 2000 placed on the fixing plate. The fixing plate 210 includes through holes 211 provided on a first side and a second side. Under the pushing force of the pushing mechanism, the electrophoretic chip 2000 is placed close to the fixing plate 210.

[0060] The fixing plate 210 is fixedly mounted on the base plate 100. The electrophoretic chip 2000 is vertically mounted and, under the push of the pushing mechanism, is placed tightly against the fixing plate 210. A single electrophoretic chip 2000 can be used, with one side of the chip receiving a pushing force from the pushing mechanism and the other side receiving a force from the fixing plate 210. Multiple electrophoretic chips 2000 can also be used, with each chip attached to the other. One chip in contact with the pushing mechanism receives a pushing force from the mechanism, while another chip in contact with the fixing plate 210 is placed tightly against the fixing plate 210 under the push of the mechanism.

[0061] When the electrophoresis chip 2000 is initially in the analysis station, it may be in a slightly tilted state. The second driving member 221 drives the pushing member 222 to move, and the pushing member 222 provides a thrust to the electrophoresis chip 2000. The electrophoresis chip 2000 is subjected to the thrust from one side and the force of the fixing plate 210 on the other side, and rotates slightly to become vertical and is placed in close contact with the fixing plate 210.

[0062] When one electrophoresis chip 2000 is set up, it is placed vertically. The second driving component 221 drives the pushing component 222 to move, providing a thrust to one side of the electrophoresis chip 2000, while the other side of the electrophoresis chip 2000 is in close contact with the fixing plate 210. After the sample to be tested in the electrophoresis chip 2000 has been analyzed, the second driving component 221 drives the pushing component 222 to move, and the electrophoresis chip 2000 loses the thrust provided by the pushing component 222, entering a released state. The blocking mechanism 500 then moves, causing the electrophoresis chip 2000 to fall to the recycling station. Then, the next electrophoresis chip 2000 is placed, and the analysis and recycling process begins.

[0063] When multiple electrophoresis chips 2000 are arranged, they are placed vertically. The second driving component 221 drives the pushing component 222 to move, providing a pushing force to the electrophoresis chips 2000 in contact with it. The fixing plate 210 provides a force to the electrophoresis chips 2000 in contact with it. The electrophoresis chips 2000 are in close contact with each other, and the position of the electrophoresis chip 2000 closest to the fixing plate 210 is the analysis station. After the sample to be analyzed in the electrophoresis chip 2000 in the analysis station is completed, the second driving component 221 drives the pushing component 222 to move, and the electrophoresis chip 2000 in the analysis station is released. The blocking mechanism 500 moves, causing the electrophoresis chip 2000 in the analysis station to fall to the recycling station. The blocking mechanism 500 moves and resets, and the second driving component 221 drives the pushing component 222 to move again, causing the remaining electrophoresis chips 2000 to be in close contact with each other, and the next electrophoresis chip 2000 moves to the analysis station. Repeat the above steps to ensure that the next electrophoresis chip 2000 has also been analyzed and recycled. Continue this process until all electrophoresis chips 2000 have been analyzed and recycled before placing the next batch of electrophoresis chips 2000 for analysis and recycling.

[0064] In other embodiments, the chip fixing mechanism 200 may also have other design structures. For example, the chip fixing mechanism 200 may be a gripper. When the electrophoresis chip 2000 is in the analysis station, the gripper clamps the electrophoresis chip 2000 to fix it in place. After the electrophoresis chip 2000 has completed the analysis, the gripper releases the electrophoresis chip 2000, blocking the movement of the blocking mechanism 500, causing the electrophoresis chip 2000 in the analysis station to fall to the recycling station.

[0065] In this embodiment, as Figure 6 and Figure 9As shown, the pushing mechanism includes a second driving member 221 and a pushing member 222. The pushing member 222 includes a straight plate 2221 and a vertical plate 2222. The straight plate 2221 is drivenly connected to the second driving member 221. One end of the vertical plate 2222 is fixedly connected to the straight plate 2221, and the other end of the vertical plate 2222 passes through the first cavity 110 and contacts the electrophoretic chip 2000 placed on the fixing plate 210 of the chip fixing mechanism 200. The second driving member 221 can drive the pushing member 222 to move. If the second driving member 221 is a motor, the output shaft of the motor is connected to the straight plate 2221. When the motor is working, it drives the straight plate 2221 to move through the output shaft, thereby driving the pushing member 222 to move as a whole. The pushing member 222 provides thrust to the electrophoretic chip 2000 in contact with it.

[0066] To save space, the second drive unit 221 is located below the base plate 100.

[0067] refer to Figures 8 to 9 The baffle 520 is designed with a square structure, and its two sides can prevent the electrophoresis chip 2000 from falling. The baffle 520 has a through cavity 521 inside. When the vertical plate 2222 of the pusher 222 passes through the first cavity 110, it also passes through the through cavity 521 of the baffle 520, so that the pusher 222 can move freely without being affected by the baffle 520.

[0068] refer to Figure 9 The process of discarding the electrophoresis chip 2000 is described in detail below. When the electrophoresis chip 2000 is in the analysis station, the second driving component 221 drives the pushing component 222 to move in the opposite direction to direction a, so that the electrophoresis chips 2000 are in close contact with each other. The electrophoresis mechanism 300 performs electrophoretic separation on the sample to be tested within the electrophoresis chip 2000 in the analysis station, and the imaging mechanism 400 then takes an image of the sample to be tested. Then, the second driving component 221 drives the pushing component 222 to move in direction a, and the electrophoresis chip 2000 loses the thrust provided by the pushing component 222. The first driving component 510 drives the baffle 520 to move in direction a, causing the electrophoresis chip 2000 to fall into the recycling station.

[0069] In this embodiment, reference Figure 3 The fixing plate 210 includes through holes 211 provided on a first side and a second side. (See reference) Figure 4 The electrophoresis apparatus 300 includes an electrophoresis fixation plate 310 and a probe. (Reference) Figure 2 The electrophoresis fixing plate 310 is adjacent to the fixing plate 210 of the chip fixing mechanism 200. The first and second sides of the electrophoresis fixing plate 310 are provided with probe holes 311 for accommodating probes. The probe holes 311 correspond to the through holes 211 on the fixing plate 210 of the chip fixing mechanism 200.

[0070] In some embodiments, the first side and the second side of the fixing plate 210 are the upper side and the lower side of the fixing plate 210, respectively, and the first side and the second side of the electrophoresis fixing plate 310 are the upper side and the lower side of the electrophoresis fixing plate 310, respectively.

[0071] The electrophoresis fixation plate 310 is fixedly mounted on the base plate 100 and is disposed adjacent to the fixation plate 210. The probe is fixedly mounted in the probe holes 311 on the first and second sides of the electrophoresis fixation plate 310 and passes through the through holes 211 on the first and second sides of the fixation plate 210.

[0072] When the electrophoresis chip 2000 is in the analysis station, the probe contacts the electrophoresis chip 2000 and applies voltage to the electrophoresis chip 2000, thereby causing the sample to be tested to be separated by electrophoresis within the electrophoresis chip 2000.

[0073] In this embodiment, reference Figure 5 The imaging mechanism 400 in the analysis device 10 includes a camera 410 and at least one light source 420. After the electrophoresis mechanism 300 performs electrophoretic separation on the sample to be tested within the electrophoresis chip 2000, the light source 420 provides light, the camera 410 takes an image of the sample to be tested within the electrophoresis chip 2000, and analyzes the image.

[0074] In this embodiment, two light sources 420 are provided, emitting light of different wavelengths. In some embodiments, one light source 420 emits light of a first wavelength, exciting a first optically detectable mark on the sample to generate a first optical signal, and a camera 410 captures the first optical signal to form an image of the sample. The other light source 420 emits light of a second wavelength, exciting a second optically detectable mark on the ladder to generate a second optical signal, and a camera 410 captures the second optical signal to form an image of the ladder. The first and second optically detectable marks may be the same or different.

[0075] In this embodiment, reference Figure 5 and Figure 10 The base plate 100 is provided with a second cavity 120. The imaging mechanism 400 includes a first guide rail 430 provided on the base plate 100, a support plate 440 provided on the first guide rail 430, a third drive member 450 provided below the base plate 100, and a connecting plate 460 with one end connected to the third drive member 450 and the other end passing through the second cavity 120 and connected to the support plate 440. Under the driving action of the third drive member 450, the connecting plate 460 drives the support plate 440 to move on the first guide rail 430. The camera 410 and the light source 420 are provided on the support plate 440.

[0076] The third driving component 450 can drive the connecting plate 460 to move. If the third driving component 450 is a motor, the output shaft of the motor is connected to the connecting plate 460, and when the motor is working, it drives the connecting plate 460 to move through the output shaft.

[0077] To save space, the third drive unit 450 is located below the base plate 100.

[0078] A slider can be mounted on the first guide rail 430, and a support plate 440 is mounted on the slider. When the third driving member 450 moves the support plate 440, the support plate 440 moves the slider on the first guide rail 430.

[0079] The third driving component 450 moves the connecting plate 460, which in turn moves the carrier plate 440 on the first guide rail 430. The carrier plate 440 then moves the camera 410 and the light source 420, enabling the camera 410 to take pictures of different positions on the electrophoresis chip 2000. Because each flow channel 2140 operates independently, the camera 410 does not need to wait for all flow channels 2140 to complete electrophoresis and can take pictures of the ready flow channels 2140.

[0080] In this embodiment, as Figure 6 As shown, the sample loading mechanism 600 includes a sample holder 610 and a sample loading assembly for extracting the sample to be tested from the sample holder 610 and loading the sample to be tested into the flow channel 2140 of the electrophoresis chip 2000.

[0081] The sample storage device 610 is mounted on the base plate 100 and includes a sample storage base and a multi-well plate mounted on the sample storage base, such as a 96-well plate.

[0082] The sample loading component moves to the corresponding position of the sample to be tested on the multi-well plate, extracts the sample, and then moves to the corresponding position on the electrophoresis chip 2000 to load the sample into the flow channel 2140 of the electrophoresis chip 2000. Multiple operations of the sample loading component can load different samples into different flow channels 2140 of the electrophoresis chip 2000.

[0083] In some specific embodiments of this application, such as Figure 1 and Figure 6 As shown, the sample loading assembly includes: a sampler 621, a first driving component 622, a second driving component 623, and a third driving component 624.

[0084] The sampler 621 is mounted on the first drive assembly 622, such as... Figure 10 This includes a puncture needle 6211 and a sample application needle 6212 disposed on the first drive assembly 622. The first drive assembly 622 drives the sample application device 621 in a first direction (e.g., Figure 1The first drive assembly 622 is disposed on the second drive assembly 623, and the second drive assembly 623 drives the first drive assembly 622 and the sampler 621 to move in the second direction (e.g., the z-direction shown); the first drive assembly 622 is disposed on the second drive assembly 623, and the second drive assembly 623 drives the first drive assembly 622 and the sampler 621 to move in the second direction (e.g., the z-direct Figure 1 The second drive component 623 is mounted on the third drive component 624, and the third drive component 624 drives the second drive component 623, the first drive component 622, and the third drive component 624 in the third direction (e.g., the y-direction shown); the second drive component 623 is mounted on the third drive component 624, and the third drive component 624 drives the second drive component 623, the first drive component 622, and the third drive Figure 1 The sampler 621 moves in the x-direction (as shown). This allows the sampler 621 to move in the first, second, and third directions, enabling it to successfully complete the sample dispensing operation.

[0085] In some specific embodiments of this application, reference is made to Figure 6 , Figure 10 and Figure 11 The first drive assembly 622 includes a first moving plate 6221, a second guide rail 6222, a first synchronous belt 6223, a first driving wheel 6224, a first driven wheel 6225, and a fourth drive member 6226. The second drive assembly 623 includes a second moving plate 6231, a third guide rail 6232, a second synchronous belt 6233, a second driving wheel 6234, a second driven wheel 6235, and a fifth drive member 6236. The third drive assembly 624 includes a fourth guide rail 6241, a third synchronous belt 6242, a third driving wheel 6243, a third driven wheel 6244, and a sixth drive member 6245.

[0086] Two fourth guide rails 6241 are provided, one on each side of the base plate 100. A second movable plate 6231 is mounted on the fourth guide rail 6241 and fixedly connected to the third synchronous belt 6242. The third synchronous belt 6242 is arranged around the third driving wheel 6243 and the third driven wheel 6244. The third driving wheel 6243 and the third driven wheel 6244 are mounted on the base plate 100. A sixth driving member 6245 drives and connects to the third driving wheel 6243. The third guide rail 6232 is mounted on the second movable plate 6231. A first movable plate 6221 is mounted on the third guide rail 6232 and fixedly connected to the second synchronous belt 6233. The second synchronous belt 6233 is arranged around the second... A driving wheel 6234 and a driven wheel 6235 are provided. The second driving wheel 6234 and the second driven wheel 6235 are provided on the second moving plate 6231. A fifth driving member 6236 drives and connects to the second driving wheel 6234. A second guide rail 6222 is provided on the first moving plate 6221. A sampler 621 is provided on the second guide rail 6222 and is fixedly connected to the first synchronous belt 6223. The first synchronous belt 6223 is provided around the first driving wheel 6224 and the first driven wheel 6225. The first driving wheel 6224 and the first driven wheel 6225 are provided on the first moving plate 6221. A fourth driving member 6226 drives and connects to the first driving wheel 6224.

[0087] The fourth driving component 6226 can drive the first driving wheel 6224 to rotate. If the fourth driving component 6226 is a motor, the output shaft of the motor is connected to the first driving wheel 6224. When the motor is working, it drives the first driving wheel 6224 to rotate through the output shaft.

[0088] The fifth driving component 6236 can drive the second driving wheel 6234 to rotate. If the fifth driving component 6236 is a motor, the output shaft of the motor is connected to the second driving wheel 6234. When the motor is working, it drives the second driving wheel 6234 to rotate through the output shaft.

[0089] The sixth driving component 6245 can drive the third driving wheel 6243 to rotate. If the sixth driving component 6245 is a motor, the output shaft of the motor is connected to the third driving wheel 6243. When the motor is working, it drives the third driving wheel 6245 to rotate through the output shaft.

[0090] When the fourth driving component 6226 is working, it transmits power to the first driving wheel 6224. The first driving wheel 6224 rotates, driving the first synchronous belt 6223 and the first driven wheel 6225 to rotate. Since the sampler 621 is fixedly connected to the first synchronous belt 6223, the sampler 621 moves in the first direction.

[0091] When the fifth driving component 6236 is working, it transmits power to the second driving wheel 6234. The second driving wheel 6234 rotates, driving the second synchronous belt 6233 and the second driven wheel 6235 to rotate. Since the first moving plate 6221 is fixedly connected to the second synchronous belt 6233, the first moving plate 6221 moves in the second direction. The sample dispenser 621 is set on the first moving plate 6221, which also enables the sample dispenser 621 to move in the second direction.

[0092] When the sixth driving component 6245 is working, it transmits power to the third driving wheel 6243. The third driving wheel 6243 rotates, driving the third synchronous belt 6242 and the third driven wheel 6244 to rotate. Since the second moving plate 6231 is fixedly connected to the third synchronous belt 6242, the second moving plate 6231 moves upward in the third direction. The first moving plate 6221 is set on the second moving plate 6231, which also enables the second moving plate 6231 and the sampler 621 to move upward in the third direction.

[0093] The sampler 621 and the first synchronous belt 6223 can be fixedly connected by providing at least one through hole on the first synchronous belt 6223, and passing a fastener such as a screw through the through hole and into the sampler 621 to fix the two together.

[0094] The first moving plate 6221 and the second synchronous belt 6233 can be fixedly connected by providing at least one through hole on the second synchronous belt 6233, and passing a fastener such as a screw through the through hole and into the first moving plate 6221 to fix the two together.

[0095] The second movable plate 6231 and the third synchronous belt 6242 can be fixedly connected by providing at least one through hole on the third synchronous belt 6242, and passing a fastener such as a screw through the through hole and into the second movable plate 6231 to fix the two together.

[0096] A slider can be installed on the second guide rail 6222, and the sampler 621 is installed on the slider. When the fourth driving member 6226 drives the sampler 621 to move, the sampler 621 drives the slider to move on the second guide rail 6222.

[0097] A slider can be set on the third guide rail 6232. The first moving plate 6221 is set on the slider. When the fifth driving member 6236 drives the first moving plate 6221 to move, the first moving plate 6221 drives the slider to move on the third guide rail 6232.

[0098] A slider can be set on the fourth guide rail 6241, and the second moving plate 6231 is set on the slider. When the sixth driving member 6245 drives the second moving plate 6241 to move, the second moving plate 6241 drives the slider to move on the fourth guide rail 6241.

[0099] In some specific embodiments of this application, reference is made to Figure 6 The sample loading assembly also includes a pipette tip holder 630 disposed on the base plate 100. The pipette tip holder 630 is provided with a plurality of first storage cavities for placing new pipette tips and at least one second storage cavity for placing old pipette tips. The second storage cavity is provided with a slot so that the old pipette tip on the sample loading needle 6212 can be removed and dropped into the second storage cavity.

[0100] The nozzle is a tip tip. After the tip tip is attached, the sample needle 6212 can extract the sample to be tested from the sample storage 610 and load the sample to be tested into the flow channel 2140 of the chip 2000.

[0101] The flow process of the pipette 621 is described in detail below. When the chip 2000 is in the analysis station, the pipette 621 moves above the first storage chamber of the pipette tip holder 630. The pipette 621 moves downward to place the pipette tip onto the sampling needle 6212. The pipette 621 moves upward and moves above the sample holder 610. The pipette 621 moves downward to extract the sample to be tested. The sampler 621 moves upward and above the electrophoresis chip 2000. At this point, the puncture needle 6211 is positioned above the flow channel 2140 of the electrophoresis chip 2000 to be sampled. The sampler 621 moves downward, and the puncture needle 6211 punctures the electrophoresis chip 2000. The sampler 621 moves upward and slightly upward, at which point the sampler needle 6212 is positioned above the flow channel 2140 of the punctured electrophoresis chip 2000. The sampler 621 moves downward, loading the sample to be tested into the flow channel 2140. The sampler 621 moves upward and above the second storage cavity of the pipette tip holder 630. The sampler 621 moves downward and engages the pipette tip in the slot. The sampler 621 moves upward, causing the pipette tip to be removed from the sampler needle 6212 and fall into the second storage cavity. In other embodiments, the above steps can be interchanged. For example, the puncture can be performed first, then the pipette tip can be put on the sampling needle 6212, and then the sample can be taken, added, and the pipette tip can be removed. Alternatively, the pipette tip can be put on the sampling needle 6212 first, then the puncture can be performed, and then the sample can be taken, added, and the pipette tip can be removed.

[0102] Please see Figures 12 to 18 The embodiments of this application provide an electrophoresis chip 2000, including a substrate 2100 and a cover plate 2200 covering the substrate 2100.

[0103] The substrate 2100 is provided with a plurality of flow channels 2140 for accommodating the sample to be tested. Each flow channel 2140 has a sample dispensing position with a sample dispensing groove 2160. The extension direction a of the sample dispensing groove 2160 is the same as the extension direction b of the corresponding flow channel 2140.

[0104] Each flow channel 2140 has an electrode assembly 2120 at both ends. Each flow channel 2140 is offset from the electrode contact end 2211 of the corresponding electrode assembly 2120, so that the extension direction of the sample loading groove 2160 does not intersect with the vertical center line direction of the electrode contact end 2211.

[0105] In this system, the electrophoresis chip 2000 is placed vertically in the analysis position, and the two electrode assemblies 2120 located at both ends of the flow channel 2140 are the positive electrode assembly and the negative electrode assembly, respectively. The probe of the electrophoresis mechanism 300 contacts the electrode assembly 2120, generating a voltage applied to the sample to be tested in the flow channel 2140, thereby realizing the electrophoretic separation of the sample to be tested. Specifically, after the chip fixing mechanism 200 fixes the electrophoresis chip 2000, the sample loading mechanism 600 sequentially loads samples into each of the multiple channels 2140 in the electrophoresis chip 2000. The electrophoresis mechanism 300 performs electrophoresis on the channels 2140 in the electrophoresis chip 2000 where the sample loading operation has been completed, so that the test samples in the channels 2140 can be separated by electrophoresis. The imaging mechanism 400 takes pictures of each channel 2140 that has completed the electrophoresis operation to obtain the sample imaging image corresponding to each channel 2140. Then, the sample imaging images obtained by taking pictures are analyzed to obtain information such as the fragment length, concentration and integrity of the test sample. In this embodiment, the flow channel 2140 is misaligned with the electrode contact end 2211 of the corresponding electrode assembly 2120, and the extension direction of the sample loading groove 2160 does not intersect with the vertical center line direction of the electrode contact end 2211. This eliminates spatial interference, enables the parallel execution of the sample loading operation and the electrophoresis operation, and improves the detection efficiency of the electrophoresis chip 2000.

[0106] The substrate 2100 and cover plate 2200 can be made of inorganic insulating materials, organic insulating materials, polymer insulating materials, composite materials, or a combination of materials. The substrate 2100 is preferably made of polypropylene, which has good light transmittance and does not release ions from its surface in an aqueous environment. It can also minimize electroosmosis without surface treatment, thereby avoiding affecting the electrophoretic separation process of the sample to be tested.

[0107] In this embodiment, the substrate 2100 can be made of a transparent material, which can ensure that when the imaging mechanism 400 takes pictures of the sample, it can successfully obtain clear pictures without the pictures being blurry due to the substrate 2100.

[0108] In this embodiment, as Figures 12-15 The substrate 2100 of the electrophoresis chip 2000 has a first surface α and a second surface β opposite to the first surface α. Electrode holes 2110 penetrate the first surface α and the second surface β. Flow channels 2140 are disposed on the first surface α of the substrate 2100.

[0109] like Figures 16-18 The cover plate 2200 has a third surface γ and a fourth surface δ opposite to the third surface γ. The fourth surface δ of the cover plate 2200 covers the first surface α of the substrate 2100, and the printed electrode layer 2210 is disposed on the fourth surface δ of the cover plate 2200.

[0110] During the testing process, the electrophoresis chip 2000 is placed vertically. The probes of the 16 channels of the electrophoresis mechanism 300 will be simultaneously inserted horizontally from the side electrode hole 2110 of the substrate 2100, passing through the second surface β and the first surface α of the substrate 2100 in sequence, and finally contacting the motor contact end 2211 on the printed electrode layer 2210 on the fourth surface δ of the cover plate 2200.

[0111] In this embodiment, as Figure 13 , Figure 14 , Figure 16 , Figure 17 As shown, the electrode assembly 2120 includes electrode holes 2110 disposed on the substrate 2100 and a printed electrode layer 2210 disposed on the cover plate 2200. (Referring to...) Figure 13 and Figure 14 When the cover plate 2200 is applied to the substrate 2100, the printed electrode layer 2210 projected onto the first surface α of the substrate 2100 is shown by dashed lines. The electrode contact ends 2211 of the printed electrode layer 2210 correspond to the electrode holes 2110, and each flow channel 2140 is offset from the electrode contact ends 2211 and electrode holes 2110 of the corresponding electrode assembly 2120. The electrode assembly 2120 adopts a structure in which the electrode holes 2110 provided on the substrate 2100 are combined with the printed electrode layer 2210 provided on the cover plate 2200, and the electrode contact ends 2211 of the printed electrode layer 2210 correspond to the electrode holes 2110, while each flow channel 2140 is offset from the electrode contact ends 2211 and electrode holes 2110 of the corresponding electrode assembly 2120. This design not only eliminates spatial interference and ensures that the sample loading and electrophoresis operations can be performed in parallel to improve detection efficiency, but also controls the consistency of the testing process conditions for each sample through the cooperation of the electrode hole 2110 and the printed electrode layer 2210 to ensure repeatability.

[0112] The surface of the substrate 2100 is generally provided with grooves and protrusions. The printed circuit solution on the substrate 2100 can easily flow into the microstructure, causing short circuits or broken lines. In order to ensure that the electrode is formed in one step and the electrode reliability, the graphene printed electrode needs to be printed on the cover plate 2200. Only the electrode hole 2110 needs to be opened on the substrate 2100, which does not involve the electrode crossing the bonding surface.

[0113] In this embodiment, the printed electrode layer 2210 is preferably a graphene printed electrode, but other inert metal electrodes, such as copper electrodes, platinum electrodes, gold electrodes, etc., can also be used. The structure and composition of the printed electrode layer 2210 are shown in the figure. Figure 16 , Figure 17 middle.

[0114] In some specific embodiments of this application, for the electrode assembly 2120 formed after the cover plate 2200 is placed over the substrate 2100, such as Figure 13 and Figure 16 As shown, the printed electrode layer 2210 includes an electrode contact end 2211, a contact end 2212, and a connecting portion 2213 for connecting the electrode contact end 2211 and the contact end 2212. The connecting portion 2213 of the printed electrode layer 2210 includes a horizontal portion 22131 and a vertical portion 22132. One end of the horizontal portion 22131 is connected to the contact end 2212, and the other end of the horizontal portion 22131 is connected to one end of the vertical portion 22132. The other end of the vertical portion 22132 is connected to the electrode contact end 2211. The horizontal portion 22131 of the connecting portion 2213 spans the buffer chamber 2150 of the flow channel 2140, and the width of the horizontal portion 22131 is greater than 1.5 mm. In order to reduce the current density on the electrode surface of the electrophoresis chip 2000, thereby reducing the risk of power failure caused by local aggregation of electrolytic bubbles, and at the same time providing sufficient space for sample aggregation after sample addition and ensuring that the graphene printed electrode can still effectively contact the buffer solution in the chamber 2150 after electrolysis, the upper edge of the horizontal part 22131 is more than 0.5 mm away from the top of the buffer solution chamber 2150, and the lower edge of the horizontal part 22131 is more than 1.5 mm away from the narrowest point of the buffer solution chamber 2150.

[0115] In other embodiments of this application, for the electrode assembly 2120 formed after the cover plate 2200 is placed over the substrate 2100, such as Figure 14 and Figure 17 As shown, the printed electrode layer 2210 includes an electrode contact end 2211, a contact end 2212, and a connecting portion 2213 for connecting the electrode contact end 2211 and the contact end 2212. The connecting portion 2213 of the printed electrode layer 2210 includes a horizontal portion 22131 and a bent portion 22133. One end of the horizontal portion 22131 is connected to the contact end 2212, one end of the bent portion 22133 is connected to the middle position of the horizontal portion 22131, and the other end of the bent portion 22133 is connected to the electrode contact end 2211.

[0116] In this embodiment, to ensure reliable thermal bonding of the substrate, the minimum bonding distance between the electrode holes 2110 and the flow channels 2140 of the electrophoretic chip 2000 is greater than 1.0 mm, ensuring a good seal between the electrode holes 2110 and the flow channels 2140. The minimum bonding distance refers to the minimum permissible distance between the centers of two adjacent bonding points, such as pads, leads, or bumps, in chip packaging or circuit board manufacturing. It is a key parameter for ensuring the reliability of the bonding process and avoiding short circuits or mechanical interference.

[0117] In this embodiment, Figure 13 , Figure 14It can be shown that the diameter of the electrode contact end 2211 is larger than the diameter of the electrode hole 2110. For example, taking the substrate 2100 as having 16 flow channels 2140 for accommodating the sample to be tested as an example, since the first surface α of the substrate 2100 has 16 flow channels 2140, and both ends of the flow channels 2140 are printed with printed electrode layers 2210, considering the positioning accuracy requirements during hot pressing, the diameter of the electrode contact end 2211 of the printed electrode layer 2210 is 0.2 mm larger than the diameter of the electrode hole 2110 on the substrate 2100, so that the electrode contact end 2211 can fully fill the electrode hole 2110 and achieve contact between the metal electrode (electrode hole 2110) and the graphene electrode (electrode contact end 2211).

[0118] In the embodiments of this application, such as Figure 13 , Figure 14 As shown, the contact end 2212 of the printed electrode layer 2210 of the electrophoresis chip 2000 corresponds to the end 2130 of the corresponding flow channel 2140. The connecting portion 2213 of the printed electrode layer 2210 bypasses the side or end 2130 of the corresponding flow channel 2140 so that the electrode contact end 2211 is misaligned with the corresponding flow channel 2140. This design eliminates spatial interference, enables parallel sample loading and electrophoresis operations to improve detection efficiency, and further optimizes the layout of the electrodes and flow channels. The bypass design can make full use of the space layout so that the electrophoresis and puncture sample loading processes in each flow channel 2140 can be performed independently.

[0119] During sample testing, when the electrophoresis chip 2000 is placed vertically, the probe of the electrophoresis mechanism 300, which performs electrophoresis on each channel 2140, is horizontally inserted into the electrode hole 2110 to contact the electrode contact end 2211 of the printed electrode layer 2210, thereby generating a voltage applied to the sample to be tested in the channel 2140 and realizing electrophoretic separation of the sample to be tested.

[0120] In some specific embodiments of this application, such as Figure 12 , Figure 13 , Figure 14As shown, in the non-edge channels of the electrophoresis chip 2000, the connecting portion 2213 of the corresponding printed electrode layer 2210 is disposed around the side or end 2130 of the corresponding channel 2140, so that the corresponding electrode contact end 2211 is located in the interval region between adjacent channels 2140. For edge channels, the connecting portion 2213 of the corresponding printed electrode layer 2210 is disposed around the side or end 2130 of the corresponding channel 2140, so that the corresponding electrode contact end 2211 is located on the side of the corresponding channel 2140 away from the adjacent channel 2140, or the electrode contact end 2211 of one edge channel 2140 is located on the side of the corresponding channel 2140 away from the adjacent channel 2140, and the electrode contact end 2211 of the other edge channel 2140 is located in the interval region between the other edge channel 2140 and the adjacent channel 2140. This design eliminates spatial interference, enabling parallel sample loading and electrophoresis operations to improve detection efficiency, and further optimizes the layout of the electrode contacts. Furthermore, placing the electrode contacts 2211 strategically in the intervening area or away from adjacent flow channels fully utilizes the internal space of the electrophoresis chip 2000, avoiding interference from the electrode contacts 2211 with the sample within the flow channel 2140, and improving electrophoretic separation. Simultaneously, this layout facilitates accurate contact between the probes of the electrophoresis mechanism 300 and the electrode contacts 2211, enhancing operational stability and reliability.

[0121] In this embodiment, the electrophoresis chip 2000 is sample tested. The sampling needle 6212 and / or puncture needle 6211 of the sampling mechanism 600, which is used to perform sample addition operation on each flow channel 2140, are vertically inserted into the sample addition groove 2160. The vertical movement path of the sampling needle 6212 and / or puncture needle 6211 does not overlap with the horizontal movement path of the probe in spatial projection.

[0122] The electrophoresis chip 2000 in this application is structurally designed so that the electrode holes 2110 and the flow channels 2140 are staggered. When one or more flow channels 2140 are undergoing electrophoresis, the remaining available flow channels 2140 can still continue to perform operations such as sample loading, sample loading waiting, and post-sample loading electrophoresis, which can save time. Since the probes passing through the electrode holes 2110 and the sample loading needles 6212 and / or puncture needles 6211 inserted into the sample loading slots 2160 are avoided, the puncture, sample loading, and electrophoresis processes among the 16 channels are all independent of each other, realizing parallel sample loading and electrophoresis operations, effectively shortening the total time required for sample imaging. By optimizing time and improving efficiency, it is also possible to control the intervals of each test process for each sample to be tested to be the same, thereby improving consistency and ensuring repeatability.

[0123] All of the above technical solutions can be combined in any way to form optional embodiments of this application, and will not be described in detail here.

[0124] The electrophoresis chip 2000 provided in this application embodiment includes a substrate 2100 and a cover plate 2200 covering the substrate 2100. The substrate 2100 is provided with a plurality of channels 2140 for accommodating samples to be tested. Each channel 2140 has a sample loading position with a sample loading groove 2160. The extension direction a of the sample loading groove 2160 is the same as the extension direction b of the corresponding channel 2140. Each channel 2140 has an electrode assembly 2120 at both ends. The electrode contact end 2211 of each channel 2140 and the corresponding electrode assembly 2120 are misaligned so that the extension direction of the sample loading groove 2160 does not intersect with the vertical center line direction of the electrode contact end 2211. In this embodiment, the flow channel 2140 is misaligned with the electrode contact end 2211 of the corresponding electrode assembly 2120, and the extension direction of the sample loading groove 2160 does not intersect with the vertical center line direction of the electrode contact end 2211. This eliminates spatial interference, enables the parallel execution of the sample loading operation and the electrophoresis operation, and improves the detection efficiency of the electrophoresis chip 2000.

[0125] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "certain examples," "specific example," or "embodiment," etc., refer to specific features, structures, materials, or characteristics described in connection with the described embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0126] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. An electrophoresis chip, characterized in that, Includes a substrate and a cover plate covering the substrate; The substrate is provided with multiple flow channels for accommodating the sample to be tested. Each flow channel has a sample dispensing slot at the sample dispensing position. The extension direction of the sample dispensing slot is the same as the extension direction of the corresponding flow channel. Each flow channel is provided with electrode assemblies at both ends, and the electrode contact ends of each flow channel and the corresponding electrode assembly are staggered so that the extension direction of the sample loading groove does not intersect with the vertical center line direction of the electrode contact end.

2. The electrophoresis chip as described in claim 1, characterized in that, The electrode assembly includes electrode holes disposed on the substrate and a printed electrode layer disposed on the cover plate; The printed electrode layer includes an electrode contact end, a contact end, and a connecting portion for connecting the electrode contact end and the contact end; The electrode contact ends of the printed electrode layer correspond to the electrode holes, and each flow channel is misaligned with the electrode contact ends and electrode holes of the corresponding electrode assembly.

3. The electrophoresis chip as described in claim 2, characterized in that, The contact end of the printed electrode layer corresponds to the end of the corresponding flow channel, and the connecting part of the printed electrode layer bypasses the side or end of the corresponding flow channel so that the electrode contact end is misaligned with the corresponding flow channel. During sample testing, when the electrophoresis chip is placed vertically, the probe of the electrophoresis mechanism used to perform electrophoresis on each channel is horizontally inserted into the electrode hole to contact the electrode contact end of the printed electrode layer.

4. The electrophoresis chip as described in claim 3, characterized in that, For non-edge channels, the corresponding connection portion of the printed electrode layer is provided to bypass the side or end of the corresponding channel so that the corresponding electrode contact end is located in the interval area between adjacent channels. For edge channels, the connecting portion of the corresponding printed electrode layer is arranged around the side or end of the corresponding channel, so that the corresponding electrode contact end is located on the side of the corresponding channel away from the adjacent channel, or the electrode contact end corresponding to one edge channel is located on the side of the corresponding channel away from the adjacent channel, and the electrode contact end corresponding to another edge channel is located in the interval area between the other edge channel and the adjacent channel.

5. The electrophoresis chip as described in claim 3, characterized in that, During sample testing, the sampling needle and / or puncture needle of the sampling mechanism used to perform sample application to each flow channel are vertically inserted into the sampling groove, and the vertical movement path of the sampling needle and / or the puncture needle does not overlap with the horizontal movement path of the probe in spatial projection.

6. The electrophoresis chip as described in claim 2, characterized in that, The substrate has a first surface and a second surface opposite to the first surface, the electrode hole penetrates the first surface and the second surface, and the flow channel is disposed on the first surface of the substrate; The cover plate has a third surface and a fourth surface opposite to the third surface, the fourth surface of the cover plate covering the first surface of the substrate, and the printed electrode layer disposed on the fourth surface of the cover plate.

7. The electrophoresis chip as described in claim 2, characterized in that, The minimum bonding distance between the electrode hole and the flow channel is greater than 1.0 mm.

8. The electrophoresis chip as described in claim 2, characterized in that, The diameter of the electrode contact end is larger than the diameter of the electrode hole.

9. The electrophoresis chip as described in claim 2, characterized in that, The connection portion of the printed electrode layer includes a horizontal portion and a vertical portion. One end of the horizontal portion is connected to the contact end, and the other end of the horizontal portion is connected to one end of the vertical portion. The other end of the vertical portion is connected to the electrode contact end; or... The connection portion of the printed electrode layer includes a horizontal portion and a bent portion. One end of the horizontal portion is connected to the contact end, one end of the bent portion is connected to the middle position of the horizontal portion, and the other end of the bent portion is connected to the electrode contact end.

10. The electrophoresis chip as described in claim 9, characterized in that, The horizontal portion of the connector spans the buffer chamber of the flow channel, and the width of the horizontal portion is greater than 1.5 mm.

11. The electrophoresis chip as described in claim 10, characterized in that, The upper edge of the horizontal portion is more than 0.5 mm from the top of the buffer chamber, and the lower edge of the horizontal portion is more than 1.5 mm from the narrowest point of the buffer chamber.

12. The electrophoresis chip as described in claim 2, characterized in that, The printed electrode layer is a graphene printed electrode.

13. An analytical apparatus, characterized in that, include: A chip fixing mechanism includes at least one analysis station, the analysis station being used to vertically place the electrophoretic chip as described in any one of claims 1-12; A sample dispensing mechanism is used to sequentially dispense samples into each channel of the flow channel; An electrophoresis apparatus is used to perform electrophoresis on the flow channel after the sample loading operation is completed, so that the test sample in the flow channel is separated by electrophoresis. as well as An imaging mechanism is used to photograph each channel after the electrophoresis operation has been completed in order to obtain an image of the sample corresponding to each channel.

14. The analytical apparatus according to claim 13, characterized in that, The chip fixing mechanism includes: Fixed plate; and A pushing mechanism provides thrust to the electrophoretic chip placed on the fixed plate, and the electrophoretic chip is placed in close contact with the fixed plate under the push of the pushing mechanism.

15. The analytical apparatus according to claim 14, characterized in that, The propulsion mechanism includes: Second drive unit; and The pusher includes a straight plate and a vertical plate. The straight plate is driven to be connected to the second drive member. One end of the vertical plate is fixedly connected to the straight plate, and the other end of the vertical plate is in contact with the electrophoresis chip placed on the fixed plate.

16. The analytical apparatus according to claim 14, characterized in that, The fixing plate includes through holes on a first side and a second side. The electrophoresis mechanism includes an electrophoresis fixing plate and a probe. The electrophoresis fixing plate is disposed adjacent to the fixing plate. The first side and the second side of the electrophoresis fixing plate are provided with probe holes for accommodating the probe. The probe holes correspond to the through holes.

17. The analytical apparatus according to claim 13, characterized in that, The imaging mechanism includes a camera and a light source.

18. The analytical apparatus according to claim 17, characterized in that, The imaging mechanism further includes a first guide rail, a support plate disposed on the first guide rail, and a third driving component drivenly connected to the support plate. The camera and the light source are both disposed on the support plate.

19. The analytical apparatus according to claim 13, characterized in that, The sample loading mechanism includes a sample holder and a sample loading component for extracting the sample to be tested from the sample holder and loading the sample to be tested into the flow channel of the electrophoresis chip.

20. The analytical apparatus according to claim 19, characterized in that, The sampling assembly includes: Pipette; A first driving component, wherein the sampler is disposed on the first driving component, and the first driving component drives the sampler to move in a first direction; A second drive component is provided, wherein the first drive component is disposed on the second drive component, and the second drive component drives the first drive component to move in a second direction; and A third drive component, wherein the second drive component is disposed on the third drive component, and the third drive component drives the second drive component to move upward on the third side.

Images