A gel making plate and a gel making device applied to a vertical electrophoresis tank

Abstract

The utility model belongs to the electrophoresis gel technical field provides a kind of glue plate and be applied to the glue preparation device of vertical electrophoresis tank, glue plate mainly includes first plate body, is equipped with upper end and lower end, the lower end side of first plate body is equipped with the first slot of through itself, first slot is equipped with preset length;First slot has first side, first side is close to the upper end side of first plate body and is set, first side has first end, second end and intermediate place;Wherein, the first end of first side, second end is close to the upper end side of first plate body and is set, the intermediate place of first side is away from the upper end side of first plate body and is set, to make first side from the upper end side of first plate body to the lower end side of first plate body protrude. By adopting above-mentioned structure, the "smile phenomenon" of protein separation band produced in the process of electrophoresis can be avoided, so that the edge resistance of the two ends of first slot and the resistance of the middle are the same, to ensure that the speed of protein separation running is consistent.

Description

Technical Field

[0001] This utility model belongs to the field of electrophoretic gel technology, and specifically refers to a gel preparation plate and a gel preparation device used in a vertical electrophoresis tank. Background Technology

[0002] Electrophoresis tanks are commonly used in biochemical experiments to separate protein molecules of different sizes from a mixture using the steric hindrance of a polyacrylamide gel's spatial grid. In operation, a gel plate containing polyacrylamide gel is fixed to an electrode holder in a vertical electrophoresis tank, forming a closed inner cavity. Electrophoresis buffer is then added to fill the closed inner cavity of the electrode holder, while a specific electrophoresis buffer is added above the anode platinum electrode in the outer tank, forming a current loop connecting the anode electrode in the outer tank, the polyacrylamide gel, the cathode platinum electrode in the electrode holder cavity, and the DC power supply. After adding the mixed protein sample, the DC power supply is turned on. Proteins of different molecular sizes, acting as anions, move towards the anode in the polyacrylamide gel, ultimately achieving separation.

[0003] Gel apparatus is commonly used in electrophoresis tank experiments. It mainly consists of two baffles and side baffles, forming a gel cavity with an open top. The gel cavity is filled with gel. After the gel is fixed, the comb is removed, and the sample is added. Then, the gel apparatus with the sample is placed into the electrophoresis tank for fixation. Combined with the inner and outer buffers in the electrophoresis tank, a circuit is formed, thereby achieving protein separation.

[0004] In existing technologies, an edge effect occurs during electrophoresis, where the gel resistance near the baffles is greater than that in the center of the gel cavity. This results in a higher current in the center of the cavity compared to the sides, causing samples in the center to move faster during separation, while samples on the sides move slower, creating a "smile phenomenon" in electrophoresis and affecting the accuracy of experimental results. Therefore, it is necessary for those skilled in the art to address this problem. Utility Model Content

[0005] This invention provides a gel preparation plate that solves the edge effect that occurs during electrophoretic separation in the aforementioned background art, ensuring that the separation speed of the sample in the middle of the gel cavity is consistent with that of the samples on both sides of the gel cavity, thus avoiding the electrophoretic "smile phenomenon". This invention also provides a gel preparation device for use in a vertical electrophoresis tank.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: A type of adhesive sheet, comprising: The first plate has an upper end and a lower end. The lower end of the first plate has a first slot that passes through itself and has a preset length. The first slot has a first side, which is located near the upper end of the first plate. The first side has a first end, a second end, and a middle section. The first end and the second end of the first side are located near the upper end of the first plate, and the middle part of the first side is located away from the upper end of the first plate, so that the first side protrudes from the upper end of the first plate towards the lower end of the first plate.

[0007] In some embodiments, a first distance is provided between the first end of the first side and the middle of the first side in a first direction, and a second distance is provided between the second end of the first side and the middle of the first side in a first direction, wherein the first distance and the second distance are equal.

[0008] In some embodiments, the first slot has a second side, which is located near the lower end of the first plate. The second side is a straight structure and is perpendicular to the first direction.

[0009] In some embodiments, a preset distance is provided between the first side and the upper end of the first plate, and the preset distance gradually increases from both ends of the first side towards the middle.

[0010] In some embodiments, the present invention also provides a gel-forming device for use in a vertical electrophoresis tank, comprising: The adhesive sheet described in each of the above embodiments; A second plate is disposed on the first plate, and a preset gap is provided between the second plate and the first plate to form a first cavity. The first cavity has a first opening on the upper side near the first plate, and the first slot communicates with the first cavity.

[0011] In some embodiments, the first plate and the second plate can be separated from each other.

[0012] In some embodiments, the first plate and the second plate are parallel to each other.

[0013] In some embodiments, a first spacer strip is also included, which is disposed between the first plate and the second plate, and the first spacer strip abuts against both the first plate and the second plate.

[0014] In some embodiments, a portion of the first spacer overlaps with the second side edge of the first slot.

[0015] In some embodiments, the first partition strip further includes a first sub-partition strip and a second sub-partition strip with a preset width, wherein the length direction of the first sub-partition strip and the length direction of the second sub-partition strip are both consistent with the first direction.

[0016] In some embodiments, the edge lines of the first sub-spacer and the second sub-spacer are tangent to both ends of the first slot.

[0017] In some embodiments, the second plate has a first notch at the first opening, the first notch has a first edge line, and the first edge line and the upper end of the first plate have a third distance in the first direction.

[0018] In some implementations, the first edge line is positioned perpendicular to the first direction.

[0019] In some embodiments, the side of the first sub-spacer protrudes or is flush with the side of the first notch, and / or the side of the second sub-spacer protrudes or is flush with the side of the first notch.

[0020] In some embodiments, the edges of the first plate and the second plate coincide, and the outer edge of the first spacer coincides with the edge of the first plate.

[0021] Compared with the prior art, the beneficial effects of this utility model are: This application provides a first through-hole at the lower end of a first plate, and a first sidewall near the upper end of the first plate near the first through-hole. The first and second ends of the first sidewall are positioned near the upper end of the first plate, while the middle portion of the first sidewall is positioned away from the upper end of the first plate, causing the first sidewall to protrude from the upper end of the first plate towards the lower end. By employing this method, the edge effect problem (the resistance at the two sides of the first cavity is greater than the resistance at the middle) in the gel-containing first cavity during electrophoresis can be solved, ensuring a consistent protein separation speed during electrophoresis and avoiding the "smile phenomenon" in electrophoresis, resulting in more accurate and reliable experimental results.

[0022] Additional aspects and advantages of this application will be set forth in part in the description which follows, and will become apparent from the description or may be learned by practice of this application. Attached Figure Description

[0023] Figure 1 This is a front view of a glue-making sheet according to the present invention; Figure 2 for Figure 1 Enlarged view of point A in the middle; Figure 3This is a schematic diagram of the structure of the first slot of this utility model; Figure 4 This is a first perspective view of the gel-making device of the present invention applied to a vertical electrophoresis tank; Figure 5 This is a second perspective view of the gel-making device of the present invention applied to a vertical electrophoresis tank; Figure 6 This is an exploded view of the gel-making device of this utility model applied to a vertical electrophoresis tank; Figure 7 This is a front view of the second plate of this utility model; Figure 8 This is a front view of the first spacer of this utility model; Figure 9 This is a front view of the first partition bar of this utility model being disposed on the first plate. Figure 10 for Figure 4 Enlarged view at point B in the middle; Figure 11 This is a schematic diagram of the second form of the first slot of this utility model; Figure 12 This is a schematic diagram of the third form of the first groove of this utility model. Detailed Implementation

[0024] The present application will be further described in detail below with reference to the accompanying drawings. In the description of the embodiments, unless otherwise stated, the terms "left", "right", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and are not intended to indicate or imply that the present application must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the present application.

[0025] like Figure 1 and Figure 2 As shown, this utility model provides a glue-making sheet, mainly comprising a first plate body 100, which has an upper end 101 and a lower end 102. A first groove 103 is provided on one side of the lower end 102 of the first plate body 100, the first groove 103 penetrating the first plate body 100 itself, and the first groove 103 having a preset length. The length direction of the first groove 103 is horizontal, as shown... Figure 2 As shown, during the electrophoresis experiment, the protein separation direction is perpendicular to the length direction of the first groove 103.

[0026] The first slot 103 has a first side 1031, which is located near the upper end 101 of the first plate 100. The first side 1031 has a first end 10311, a second end 10312, and a middle portion 10310. The first end 10311 and the second end 10312 of the first side 1031 are located near the upper end 101 of the first plate 100, while the middle portion of the first side 1031 is located away from the upper end 101 of the first plate 100. Specifically, the middle portion 10310 of the first side 1031 is located near the lower end of the first plate 100. Figure 3 As shown, this causes the first side 1031 to protrude from the upper end 101 side of the first plate 100 towards its lower end 102 side, as... Figure 2 and Figure 3 As shown in the image.

[0027] In this application, by adopting the above-described structure for the first side 1031 of the first groove 103, the colloidal resistance at both ends of the first side 1031 is made consistent with the resistance at the middle during electrophoresis experiments. As a result, the protein separation speed at both ends of the first side 1031 is consistent with the protein separation speed at the middle 10310 during electrophoresis experiments, thus avoiding the "smile phenomenon" in existing electrophoresis experiments and ensuring the accuracy of experimental data.

[0028] In one embodiment, such as Figure 3 As shown, a first distance 1033 is provided between the first end 10311 and the middle portion 10310 of the first side 1031 in the first direction, and a second distance 1034 is provided between the second end 10312 and the middle portion 10310 of the first side 1031 in the first direction, wherein the first distance 1033 and the second distance 1034 are equal. In this embodiment, the first direction is the direction in which the protein separation is carried out perpendicular to the electrophoresis. The first plate 100 has a rectangular structure, and the first direction is perpendicular to the upper edge line of the first plate 100 and parallel to the side edge line of the first plate 100, as shown. Figure 1 As shown.

[0029] Due to the edge effect of protein separation during electrophoresis, the resistance at both ends of the first groove 103 in the first direction is greater than the resistance at the middle part 10310 in the first direction. This results in slower protein separation at both ends of the first groove 103 and faster protein separation in the middle, creating the electrophoretic "smile phenomenon". By setting the first spacing 1033 and the second spacing 1034 to be equal, the resistance at both ends of the first groove 103 is ensured to be the same as the resistance at the middle part 10310. This ensures that the protein separation speed at both ends and the protein separation speed at the middle part 10310 are consistent during the electrophoresis experiment, forming a rectangular multi-band protein separation pattern and ensuring the accuracy of the experimental data.

[0030] Preferably, in this embodiment, the size of the first spacing 1033 and the second spacing 1034 is both 1mm to achieve the best effect. Optionally, the size of the first spacing 1033 and the second spacing 1034 can also be other distances, such as 0.9mm, 0.8mm, 1.1mm or 1.2mm, etc., which can be preset according to actual experimental needs.

[0031] In one embodiment, such as Figure 3 As shown, the first slot 103 also has a second side 1032, which is located near the lower end 102 of the first plate 100. The second side 1032 and the first side 1031 are located on opposite sides of the first slot 103, which in this embodiment are the upper and lower sides in the first direction. To reduce the space occupied by the first slot 103 at the lower end of the first plate 100, and because part of the first spacer 300 needs to overlap with the second side 1032, as shown... Figure 9 As shown, in order to facilitate the processing and assembly of the first spacer 300, the second side 1032 is set as a straight line structure, and the second side 1032 is perpendicular to the first direction.

[0032] Alternatively, if assembly difficulty and machining difficulty of the first spacer 300 are not considered, the second side 1032 can also be as follows: Figure 11 and Figure 12 Non-linear structures in [the text].

[0033] In one embodiment, a preset distance is provided between the first side 1031 and the upper end 101 of the first plate 100 in a first direction. This preset distance gradually increases from both ends of the first side 1031 towards the middle 10310. In this embodiment, the upper end 102 of the first plate 100 is the upper edge line of the first plate 100. Since the upper edge line of the first plate 100 in this application is a straight line and is perpendicular to the first direction in which the electrophoretic protein separation occurs, it can be used as a reference. Optionally, when the upper edge line of the first plate 100 is not a straight line, it is not used as a precise reference. An arbitrary straight line can be set on the first plate 100 as a reference. This straight line must meet the following conditions: it is set on the side close to the upper end 101 of the first plate 100, there is a certain distance between it and the first side 1031, and the straight line is perpendicular to the first direction. Thus, it can be used as a reference. By setting the preset spacing to gradually increase from the first end 10311 and the second end 10312 towards the middle 103101, it is ensured that the resistance of the gel at the first end 10311 in the first direction is the same as that of the gel at the middle 10310 in the first direction, and the resistance of the gel at the second end 10312 in the first direction is the same as that of the gel at the middle 10310 in the first direction. This ensures that the gel resistance in the first direction of the span from the first end 10311 to the second end 10312 of the first side 1031 is the same, thus ensuring that the protein separation speed is consistent during the electrophoresis experiment, and ultimately the multiple protein separation bands form a rectangular structure.

[0034] In this embodiment, the gradually increasing preset spacing is reflected in the fact that the first end 10311 of the first side 1031 to the middle 10310 is a straight line structure, and the second end 10312 of the first side 1031 to the middle 10310 is a straight line structure.

[0035] Optionally, the first end 10311 to the middle 10310 can also be a gentle curve structure, and the second end 10312 to the middle 10310 can also be a gentle curve structure, or a wavy line structure.

[0036] It should be noted that, regardless of the structural shape of the two ends to the middle 10310 of the first slot 103, it is symmetrical about the center line of the first side 10310 in the first direction.

[0037] In one embodiment, such as Figure 4 and Figure 5 as well as Figure 6As shown, this utility model also provides a gel-making device for a vertical electrophoresis tank, mainly including the gel-making plate in the above embodiments. The gel-making plate is a first plate 100 with a first groove 103; a second plate 200 is disposed on the first plate 100, and a preset gap is provided between the second plate 200 and the first plate 100 to form a first cavity. The first cavity is used to pour gel to prepare the gel required for the electrophoresis experiment. A first opening 201 is provided on the upper end 101 of the first cavity near the first plate 100. The first opening 201 is used for pouring gel during gel preparation and also for inserting the comb required for gel preparation, so as to form the sample placement hole required for the experiment on the gel. It should be noted that the first groove 103 connects to the first cavity, thereby realizing the conduction of the positive and negative electrodes of the electrode holder during the electrophoresis experiment, forming the electric field required for electrophoresis.

[0038] Furthermore, in this embodiment, a homemade adhesive is used. Therefore, two separate first plates 100 and second plates 200 are used to make the gel. The first plates 100 and second plates 200 are fixed in a specific clamp (adhesive clamp). At this time, the first plate 100 is generally made of glass, but it can also be made of acrylic plastic.

[0039] Optionally, when using pre-formed adhesive, the first plate 100 and the second plate 200 are integrally formed and made of plastic material, such as transparent acrylic sheet.

[0040] Furthermore, in order to ensure the uniformity of the gel, the first plate 100 and the second plate 200 are arranged parallel to each other, thereby ensuring that the gel thickness in the first cavity is consistent, and thus ensuring that the resistance from the first groove 103 to the first opening 201 is the same in the first direction, ensuring that the protein separation speed is consistent during the electrophoresis experiment.

[0041] In one embodiment, to form the first cavity, the first plate 100 and the second plate 200 are arranged parallel to each other at intervals. A first spacer 300 is provided between the first plate 100 and the second plate 200. The first spacer 300 has a certain thickness and abuts against the first plate 100 and the second plate 200 respectively, thereby forming a preset gap between the first plate 100 and the second plate 200. In this embodiment, the first spacer 300 has a U-shaped structure and seals the three sides of the first plate 100 and the second plate 200 (except for the first opening side), thereby forming the first cavity.

[0042] Furthermore, a portion of the first spacer 300 overlaps with the edge of the second side 1032 of the first slot 103, ensuring a seal while preventing gel from flowing below the second side 1032 of the second slot 103.

[0043] In one embodiment, such as Figure 8As shown, the first partition 300 includes a first sub-partition 301, a second sub-partition 302, and a third sub-partition 303, each with a preset width. The length direction of the first sub-partition 301 is parallel to the first direction, the length direction of the second sub-partition 302 is parallel to the first direction, and the length direction of the third sub-partition 303 is perpendicular to the first direction. The edge of the third sub-partition 303 coincides with the second side 1032 of the first slot 103. Figure 9 As shown. In this example, the first sub-spacer 301, the second sub-spacer 302, and the third sub-spacer 303 are integrally constructed.

[0044] Optionally, the first sub-spacer 301, the second sub-spacer 302, and the third sub-spacer 303 can be independent entities. In this embodiment, for ease of assembly, the first spacer 300 is bonded to the first plate 100. Optionally, the first spacer 300 can also be bonded to the second plate 200. The first spacer 300 can be integrally formed with the first plate 100, or the first spacer 300 and the second plate 200 can be integrally formed.

[0045] In one embodiment, the interior of the first groove 103 is a smooth structure. In this embodiment, rounded corners are used for smooth connection, thereby simplifying the processing technology of the first groove 103 and reducing the processing difficulty of the first groove 103.

[0046] Furthermore, such as Figure 9 As shown, the edge of the first sub-spacer 301 near the first cavity is tangent to the first end 10311 of the first slot 103, and the edge of the second sub-spacer 302 near the first cavity is tangent to the second end 10312 of the first slot 103. This ensures that the length range of the first slot 103 covers the span range of the first cavity, which refers to the span range perpendicular to the first direction. This ensures that the running width of the gel in the electrophoresis experiment is the length of the first slot 103, thus ensuring the accuracy of the experiment.

[0047] In one embodiment, such as Figure 7 As shown, the second plate 200 has a first notch 2011 at the first opening 201, the first notch 2011 has a first edge line, and the first edge line and the upper end of the first plate 100 have a third distance in the first direction. During the electrophoresis experiment, inner and outer buffer solutions are set in the electrophoresis tank. The inner buffer solution immerses the first edge line at the first notch 2011, and the outer buffer solution immerses the first tank opening 103. Thus, the current passes through the inner buffer solution, the gel, the first tank opening 103, and the outer buffer solution to form a circuit, thereby forming the electric field required for electrophoresis.

[0048] Furthermore, the first edge line is set perpendicular to the first direction, making the first edge line horizontal, thereby ensuring that the required height of the inner buffer solution relative to the first edge line is minimized, so that the first edge line can be submerged and the amount of inner buffer solution used is reduced.

[0049] In one embodiment, such as Figure 10 As shown, the side of the first sub-spacer 301 protrudes or is flush with the side of the first notch 2011, and / or the side of the second sub-spacer 302 protrudes or is flush with the side of the first notch 2011.

[0050] Specifically, the first sub-spacer 301 can be flush with the side of the first notch 2011, or the first sub-spacer 3011 can protrude from the side of the first notch 2011; the side of the second sub-spacer 302 protrudes from the side of the first notch 2011, or the side of the second sub-spacer 302 is flush with the side of the first notch 2011. Regardless of the combination of the above situations, no gap will be formed between the second plate 200 and the first plate 100 at the edge of the first sub-spacer 301, and no gap will be formed between the second plate 200 and the first plate 100 at the edge of the second sub-spacer 302, thereby avoiding the resistance at the two sides of the first notch 2011 from affecting the accuracy of the experiment.

[0051] In one embodiment, in order to facilitate the clamping, positioning and fixing of the entire device by the adhesive frame, the edges of the first plate 100 and the second plate 200 overlap, the outer edge of the first spacer 300 overlaps with the edge of the first plate 100, and the outer edges of the first plate 100, the second plate 200 and the first spacer 300 are all planar structures.

[0052] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of this utility model. These improvements and modifications should also be considered within the scope of protection of this utility model.

Claims

1. A type of adhesive sheet, characterized in that, include: The first plate has an upper end and a lower end. The lower end of the first plate has a first slot that passes through itself and has a preset length. The first slot has a first side, which is located near the upper end of the first plate. The first side has a first end, a second end, and a middle section. The first end and the second end of the first side are located near the upper end of the first plate, and the middle part of the first side is located away from the upper end of the first plate, so that the first side protrudes from the upper end of the first plate towards the lower end of the first plate.

2. The adhesive sheet according to claim 1, characterized in that, A first distance is provided between the first end of the first side and the middle of the first side in a first direction, and a second distance is provided between the second end of the first side and the middle of the first side in a first direction, wherein the first distance and the second distance are equal.

3. The adhesive sheet according to claim 2, characterized in that, The first slot has a second side, which is located near the lower end of the first plate. The second side is a straight line and is perpendicular to the first direction.

4. The adhesive sheet according to claim 2, characterized in that, In the first direction, a preset distance is provided between the first side and the upper end of the first plate, and the preset distance gradually increases from both ends of the first side towards the middle.

5. A gel-forming device for use in a vertical electrophoresis tank, characterized in that, include: Glue sheet according to any one of claims 1-4; A second plate is disposed on the first plate, and a preset gap is provided between the second plate and the first plate to form a first cavity. The first cavity has a first opening on the upper side near the first plate, and the first slot communicates with the first cavity.

6. The gel-forming device for a vertical electrophoresis tank according to claim 5, characterized in that, The first plate and the second plate can be separated from each other.

7. The gel-forming device for a vertical electrophoresis tank according to claim 6, characterized in that, The first plate and the second plate are parallel to each other.

8. The gel-forming device for a vertical electrophoresis tank according to claim 6, characterized in that, It also includes a first spacer strip disposed between the first plate and the second plate, wherein the first spacer strip abuts against both the first plate and the second plate.

9. A gel-forming device for a vertical electrophoresis tank according to claim 8, characterized in that, Part of the first spacer overlaps with the second side edge of the first slot.

10. A gel-forming device for a vertical electrophoresis tank according to claim 8, characterized in that, The first partition also includes a first sub-partition and a second sub-partition with a preset width, wherein the length direction of the first sub-partition and the length direction of the second sub-partition are both consistent with the first direction.

11. The gel-forming apparatus for a vertical electrophoresis tank according to claim 10, characterized in that, The edge lines of the first sub-spacer and the second sub-spacer are tangent to both ends of the first slot, respectively.

12. The gel-forming apparatus for a vertical electrophoresis tank according to claim 11, characterized in that, The second plate has a first notch at the first opening, the first notch has a first edge line, and the first edge line and the upper end of the first plate have a third distance in the first direction.

13. The gel-forming device for a vertical electrophoresis tank according to claim 12, characterized in that, The first edge line is set perpendicular to the first direction.

14. The gel-forming apparatus for a vertical electrophoresis tank according to claim 12, characterized in that, The side of the first sub-spacer protrudes or is flush with the side of the first notch, and / or the side of the second sub-spacer protrudes or is flush with the side of the first notch.

15. A gel-forming device for a vertical electrophoresis tank according to claim 8, characterized in that, The edge of the first plate coincides with the edge of the second plate, and the outer edge of the first spacer coincides with the edge of the first plate.

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