Hepatocarcinoma lncrna diagnostic reagent card

Abstract

The utility model discloses a kind of liver cancer lncRNA diagnostic reagent cards, specifically relates to reagent card technical field, including: by the flat card body of the upper cover plate and lower substrate stacking composition, its inside is provided with fluid passage, the fluid passage successively passes sample injection area, lysis reaction cavity, freeze-dried reagent storage cavity and chromatography detection zone;The glass microbead is built-in in the lysis reaction cavity;The freeze-dried reagent storage cavity contains lncRNA capture probe freeze-dried ball;Chromatography detection zone is fixed with detection line and quality control line;The utility model realizes breakthrough advantage by multiple physical structure collaborative innovation: first hand-held shaking type mechanical lysis cavity is got rid of centrifuge and toxic reagent, and cell crushing is quickly completed;Innovation nanometer gold-HRP cascade amplifier improves detection sensitivity by enzymatic signal amplification, so that early liver cancer key marker MALAT1 detection rate is improved;Integrating body temperature activation layer and vacuum freeze-drying cavity realize 37 degrees Celsius passive temperature control reaction and reagent normal temperature stable storage and transportation, break through cold chain and power bondage.

Claims

1. A diagnostic reagent card for liver cancer lncRNA, characterized in that, include: A flat card body (1) is formed by stacking an upper cover plate (101) and a lower base plate (102). A fluid channel (11) is provided inside the card body (1). The fluid channel (11) passes through a sample injection area (201), a lysis reaction chamber (301), a lyophilized reagent storage chamber (401), and a chromatography detection band (501) in sequence. The sample injection area (201) is located at the short side of the card body (1) and has a capillary inlet (2011) as the fluid channel inlet. The lysis reaction chamber (301) contains glass microbeads (302). The lyophilized reagent storage chamber (401) contains lyophilized lncRNA capture probe beads (402). The chromatography detection band (501) is fixed with a detection line (502) and a control line (503).

2. The liver cancer lncRNA diagnostic reagent card according to claim 1, characterized in that, The top of the freeze-dried reagent storage chamber (401) is sealed with a vacuum-sealed aluminum foil diaphragm (403) to store the freeze-dried reagent. The bottom plate of the freeze-dried reagent storage chamber (401) is provided with a pin (404). When the card body (1) is bent downward, the pin (404) punctures the vacuum-sealed aluminum foil diaphragm (403) to release the freeze-dried reagent.

3. The liver cancer lncRNA diagnostic reagent card according to claim 1, characterized in that, The inner wall of the lysis reaction chamber (301) is provided with spiral protrusions (303), which, together with the glass microspheres (302), can achieve cell disruption by hand-shaking.

4. The liver cancer lncRNA diagnostic reagent card according to claim 1, characterized in that, The chromatographic detection band (501) includes a gold nanoparticle-labeled universal signal amplifier (504) for binding multiple lncRNA-probe complexes to perform a colorimetric reaction.

5. The liver cancer lncRNA diagnostic reagent card according to claim 1, characterized in that, The lower substrate (102) integrates a temperature activation layer (601), which is composed of a buffer reservoir. The buffer reservoir is connected to the fluid channel (11), and the outlet of the buffer reservoir is sealed with paraffin. The temperature activation layer (601) releases a pre-stored reaction buffer when it comes into contact with the body temperature of a human body.

6. The liver cancer lncRNA diagnostic reagent card according to claim 1, characterized in that, The detection line (502) is coated with streptavidin for capturing the biotinylated probe complex of the liver cancer-specific lncRNA MALAT1; the control line (503) is coated with a universal probe antibody.

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