An explosion-proof cryopreservation tube

Abstract

This application relates to an explosion-proof cryopreservation tube, belonging to the field of biomedical technology. It includes a tube body and a cap. The tube body has a solution chamber and an opening communicating with the solution chamber. The inner side of the opening facing the solution chamber has a recessed, rounded chamfer, and the outer side of the opening has a threaded groove and a recess for installing a sealing ring. The threaded groove and recess are distributed along the axial direction of the tube body. The cap is sealed to the tube body through the threaded groove, and the sealing ring abuts against the recess through the cap. An vent valve is located at the top of the cap, comprising a through hole at the top of the cap and an elastic plate covering the through hole. The edge of the elastic plate is fixed to the top surface of the cap. When the pressure inside the cryopreservation tube increases, a portion of the elastic plate bulges, forming a vent gap with the through hole. When the pressure inside the cryopreservation tube equalizes, the elastic plate springs back to seal the through hole. By incorporating the vent valve, real-time response to changes in internal pressure of the cryopreservation tube is possible, avoiding the risk of tube bursting due to pressure differential changes.

Description

Technical Field

[0001] This application relates to the field of biomedical technology, and in particular to an explosion-proof cryopreservation tube. Background Technology

[0002] In fields such as biomedical research, cell therapy, assisted reproduction, and clinical sample bank management, biological samples (such as cells, tissues, semen, and embryos) typically require long-term preservation at ultra-low temperatures to maintain their viability and stability. Currently, the widely used storage method involves encapsulating samples in cryovials and freezing them in liquid nitrogen (-196°C) or ultra-low temperature freezers (-80°C). However, in practical use, traditional cryovials have numerous safety hazards and technical defects, severely limiting their reliability and safety in storing high-value samples.

[0003] First, existing cryopreservation tubes mostly use screw caps connected to the tube body via threads and sealed with a sealing ring. However, under extreme low-temperature conditions, polypropylene material is prone to embrittlement, and microcracks often appear at the tube opening due to stress concentration, leading to tube breakage or seal failure, especially during repeated freeze-thaw cycles. Furthermore, liquid or cryoprotectant (such as DMSO) residue can easily remain at the tube opening, making thorough cleaning and disinfection difficult and potentially fostering microbial growth and cross-contamination. Second, traditional cryopreservation tubes are completely sealed. When liquid nitrogen seeps into the tube through tiny gaps or the sample contains volatile components, the liquid nitrogen rapidly vaporizes during heating, causing a sharp increase in internal pressure and easily leading to a tube burst. This can damage storage equipment and pose a safety threat to operators.

[0004] Therefore, there is an urgent need for a well-designed cryopreservation tube structure to solve the above problems. Utility Model Content

[0005] The purpose of this application is to overcome the shortcomings of the prior art and provide an explosion-proof cryopreservation tube.

[0006] To achieve the above objectives, this utility model provides an explosion-proof cryopreservation tube, which adopts the following technical solution: An explosion-proof cryopreservation tube includes a tube body and a cap. The tube body has a solution chamber and a tube opening communicating with the solution chamber. The inner side of the tube opening facing the solution chamber has a recessed arc chamfer, and the outer side of the tube opening has a threaded groove and a groove for installing a sealing ring. The threaded groove and the groove are distributed along the axial direction of the tube body. The cap is sealed to the tube body through the threaded groove, and the sealing ring is sealed and abuts against the groove through the cap. The top of the cover is provided with an exhaust valve, which includes a through hole on the top of the cover and an elastic sheet covering the through hole. The edge of the elastic sheet is fixed to the top surface of the cover. When the pressure inside the cryopreservation tube increases, a portion of the elastic sheet bulges out and forms an exhaust gap with the through hole. When the pressure inside the cryopreservation tube is balanced, the elastic sheet springs back and seals the through hole.

[0007] Furthermore, along the axial direction extending from the opening towards the bottom wall of the tube, the diameter of the groove gradually decreases.

[0008] Furthermore, the inner bottom of the cover body is provided with an annular groove, and the sealing ring includes a first fixing part and a second fixing part. The first fixing part is installed in the annular groove, and the second fixing part is an annular convex ridge structure. Along the direction away from the first fixing part, the thickness of the second fixing part gradually decreases.

[0009] Furthermore, the ratio of the diameter of the through hole to the diameter of the cover is 1:10; the elastic sheet is a circular thin sheet, the ratio of the diameter of the through hole to the diameter of the elastic sheet is 1:3, and the thickness of the elastic sheet is 0.3mm-0.5mm.

[0010] Furthermore, the elastic sheet is made of butyl rubber or silicone, and the elastic sheet and the top surface of the cover are integrally injection molded.

[0011] Furthermore, the radius of the chamfered arc is 0.1mm-0.3mm.

[0012] Furthermore, along the axial extension direction of the tube body, the groove and the threaded groove are arranged adjacent to each other, and the threaded groove is located at one end of the tube opening near the bottom of the tube body.

[0013] Furthermore, the threads of the tube body and the cover body are fine-pitch trapezoidal threads with a pitch of 0.8mm-1.2mm and a height of 0.2mm-0.4mm, and the thread tip has a flattened structure.

[0014] Furthermore, the tube body is made of transparent polypropylene with a wall thickness of 0.8mm-1.2mm, a length of 55mm-65mm, and an outer diameter of 10mm-12mm.

[0015] Furthermore, the outer wall of the tube is provided with graduation lines.

[0016] Beneficial effects: The cryopreservation tube of this application features a rounded chamfer on the inner side of the tube opening, instead of a right angle transition, avoiding the micro-cracks caused by material embrittlement at low temperatures that are common in traditional right-angle structures. Furthermore, the rounded chamfer reduces dead zones for liquid residue, facilitating cleaning and disinfection of the cryopreservation tube. A groove precisely positions the sealing ring, ensuring a tight seal between the sealing ring and the tube opening when the cap is tightened, preventing displacement during assembly or use. This provides stable pre-tightening force, enhancing static sealing performance. The sealing ring effectively blocks external microorganisms and liquid nitrogen penetration, preventing cross-contamination. In addition, the flexible vent valve responds in real-time to internal pressure changes, actively releasing high-pressure gas generated by liquid nitrogen vaporization, fundamentally eliminating the risk of tube bursting and ensuring the safety of personnel and samples. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0018] Figure 1 This is a three-dimensional structural diagram of the explosion-proof cryopreservation tube of this application; Figure 2 This is a partial structural cross-sectional view of the explosion-proof cryopreservation tube of this application.

[0019] In the diagram, 100 is the tube body; 200 is the cap; 110 is the solution chamber; 300 is the rounded chamfer; 400 is the threaded groove; 600 is the sealing ring; 610 is the first fixing part; 620 is the second fixing part; 700 is the exhaust valve; 710 is the through hole; 720 is the elastic sheet; and 800 is the scale line. Detailed Implementation

[0020] The following is in conjunction with the appendix Figure 1 - Appendix Figure 2 The technical solutions in the embodiments of this application are clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of this application.

[0021] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in the embodiments of this application are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0022] Furthermore, the use of terms such as "first," "second," etc., in this application is for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0023] In this application, unless otherwise expressly specified and limited, the terms "connection," "fixed," etc., should be interpreted broadly. For example, "fixed" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0024] Furthermore, the technical solutions of the various embodiments of this application can be combined with each other, but only if they are based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such combination of technical solutions does not exist and is not within the scope of protection claimed by this application.

[0025] This utility model discloses an explosion-proof cryopreservation tube, including a tube body 100 and a cap 200. The tube body 100 is provided with a solution chamber 110 and a tube opening communicating with the solution chamber 110. The inner side of the tube opening facing the solution chamber 110 is provided with a recessed arc chamfer 300. The outer side of the tube opening is provided with a threaded groove 400 and a groove (not shown) for installing a sealing ring 600. The threaded groove 400 and the groove are distributed along the axial direction of the tube body 100. The cap 200 is sealed to the tube body 100 through the threaded groove 400. The sealing ring 600 is sealed and abuts against the groove through the cap 200. The top of the cover 200 is provided with an exhaust valve 700. The exhaust valve 700 includes a through hole 710 on the top of the cover 200 and an elastic sheet 720 covering the through hole 710. The edge of the elastic sheet 720 is fixed to the top surface of the cover 200. When the pressure inside the cryopreservation tube increases, a portion of the elastic sheet 720 bulges out and forms an exhaust gap with the through hole 710. When the pressure inside the cryopreservation tube is balanced, the elastic sheet 720 rebounds and seals the through hole 710.

[0026] like Figure 1 , Figure 2As shown, this application aims to address the problems of high risk of tube bursting, insufficient sealing, susceptibility to contamination, and difficulty in cleaning sample residues in existing cryopreservation tubes during the low-temperature storage of biological samples. By optimizing the structural design of cryopreservation tubes, especially by improving and innovating the sealing system and venting mechanism, a new type of cryopreservation tube with strong explosion-proof performance, excellent anti-contamination capability, and safe and reliable operation is achieved, meeting the needs of cell therapy, assisted reproduction, clinical sample bank construction, and other fields for high-safety and high-reliability cryopreservation containers.

[0027] Specifically, the cryopreservation tube adopts a classic split spiral structure, consisting of a tube body 100 and a cap 200. The tube body 100 is a cylindrical hollow structure with a solution chamber 110 inside for holding biological samples. The top opening is the tube opening, which facilitates sample addition and sealing. A threaded groove 400 and a groove are provided on the outside of the tube opening, which are distributed along the axial direction and are used to realize the tightening connection of the cap 200 and the positioning and installation of the sealing ring 600, respectively. In addition, a rounded chamfer 300 is machined on the inside of the tube opening.

[0028] A through hole 710 is integrated in the center of the top of the cover 200, serving as a gas passage. An elastic sheet 720 covers the through hole 710, with its edge fixedly connected to the top surface of the cover 200. Only the central area of ​​the elastic sheet 720 can undergo elastic deformation. Under normal conditions, the elastic sheet 720 adheres tightly to the opening of the through hole 710 under its own elastic force, achieving a complete seal. When the cryopreservation tube is removed from liquid nitrogen and heated, if the residual liquid nitrogen inside vaporizes, causing the pressure to rise, the gas pushes the center of the elastic sheet 720 to bulge, forming a tiny gap between it and the opening, achieving automatic venting. After the pressure is released, the elastic sheet 720 automatically resets and reseals due to its rebound force.

[0029] The cryopreservation tube in this embodiment features a rounded chamfer of 300° on the inner side of the tube opening, instead of a right angle transition. This avoids the micro-cracks caused by material embrittlement at low temperatures that can occur with traditional right-angle structures. Furthermore, the rounded chamfer of 300° at the tube opening reduces dead zones for liquid residue, facilitating cleaning and disinfection of the cryopreservation tube. A groove is used to precisely position the sealing ring 600. When the cap 200 is tightened, it compresses the sealing ring 600 into close contact with the tube opening end face, ensuring that the sealing ring 600 will not shift during assembly or use. This provides a stable pre-tightening force, enhancing static sealing performance. The sealing ring 600 effectively blocks external microorganisms and liquid nitrogen penetration paths, preventing cross-contamination. In addition, the flexible exhaust valve 700 responds to internal pressure changes in real time, actively releasing high-pressure gas generated by liquid nitrogen vaporization, fundamentally eliminating the risk of tube bursting and ensuring the safety of personnel and samples.

[0030] In one embodiment of this utility model, the diameter of the groove gradually decreases along the axial direction extending from the opening to the bottom wall of the tube body 100; an annular groove is provided at the bottom inner side of the cover body 200; the sealing ring 600 includes a first fixing part 610 and a second fixing part 620; the first fixing part 610 is installed in the annular groove; the second fixing part 620 has an annular convex ridge structure; and the thickness of the second fixing part 620 gradually decreases along the direction away from the first fixing part 610.

[0031] Based on the basic sealing structure, this embodiment further optimizes the fit between the sealing ring 600 and the groove of the tube body 100, aiming to improve the sealing reliability and creep resistance of the cryopreservation tube under extreme temperature change conditions, and prevent liquid nitrogen infiltration, sample contamination or tube bursting accidents caused by sealing failure.

[0032] A groove for installing the sealing ring 600 is provided on the outer side of the pipe opening of the pipe body 100. Specifically, the diameter of the groove gradually decreases along the axial direction extending from the pipe opening to the bottom wall of the pipe body 100, forming a conical structure with an inner diameter decreasing from top to bottom. The sealing ring 600 is an integrally molded elastomer, including two functional areas: a first fixing part 610 and a second fixing part 620. The first fixing part 610 is located on the upper part of the sealing ring 600, and its shape matches the annular groove on the bottom inner side of the cover body 200. During assembly, it is embedded in the annular groove, which plays a role in positioning and preventing it from falling off. The second fixing part 620 is located on the lower part of the sealing ring 600, and has an annular convex ridge structure. Its thickness gradually decreases from the end near the first fixing part 610 downwards, forming a wedge-shaped or slope-shaped contact surface. When the cover 200 is tightened onto the tube 100, the first fixing part 610 of the sealing ring 600 is pressed firmly into the annular groove of the cover 200 to ensure that it will not shift. At the same time, the wedge-shaped part of the second fixing part 620 is forced into the conical groove on the tube 100. Since the two geometric shapes are complementary, a radial expansion force is generated under the action of axial pressure, so that the sealing ring 600 and the groove wall form an interference fit relationship that gets tighter and tighter as it is pressed. In addition, the sealing ring 600 can also fit further against the end face of the tube opening to enhance the end face sealing effect.

[0033] The cryopreservation tube in this embodiment features a tapered groove with a decreasing diameter from top to bottom and a wedge-shaped sealing ring 600 with a gradually varying thickness. Utilizing the wedge-tightening effect generated by the tapered surface, it maintains a stable pre-tightening force even at low temperatures, effectively compensating for gaps caused by material shrinkage and significantly improving sealing reliability. Simultaneously, the first fixing part 610 of the sealing ring 600 is embedded in the annular groove of the cap 200, ensuring accurate assembly positioning and preventing detachment. Combined with the wedge-shaped structure of the second fixing part 620, it achieves self-guided sealing, avoiding stress concentration. This design forms a dual barrier of "side sealing + end sealing," synergistically blocking the liquid nitrogen infiltration path, significantly reducing sample contamination and tube burst risks. It is particularly suitable for long-term liquid nitrogen storage scenarios, improving the sealing stability and biosafety of the cryopreservation tube under extreme conditions.

[0034] In one embodiment of this utility model, the ratio of the diameter of the through hole 710 to the diameter of the cover 200 is 1:10; the elastic sheet 720 is a circular thin sheet, the ratio of the diameter of the through hole 710 to the diameter of the elastic sheet 720 is 1:3, and the thickness of the elastic sheet 720 is 0.3mm-0.5mm; the elastic sheet 720 is made of butyl rubber or silicone, and the elastic sheet 720 and the top surface of the cover 200 are integrally injection molded.

[0035] The technical solution of this application ensures that the exhaust valve 700 maintains a tight seal under normal conditions and can respond promptly and safely to exhaust pressure when the internal pressure rises abnormally by precisely setting the dimensional ratio between the through hole 710 and the elastic sheet 720, and by selecting specific materials and molding processes. Specifically, a vertical through hole 710 with a smaller diameter is provided in the center of the top of the cover 200, with a diameter ratio of 1:10 to the outer diameter of the entire cover 200. This ensures the overall strength of the cover 200 structure and provides an effective channel for exhaust. A circular elastic sheet 720 with a larger diameter (with a diameter ratio of 1:3 to that of the through hole 710) is covered above the through hole 710. The edge of the elastic sheet 720 is fixed to the top surface of the cover 200 by injection molding, and only the central area can undergo elastic deformation.

[0036] When the pressure inside the cryopreservation tube rises, the gas pushes the elastic sheet 720 to bulge partially, creating a tiny gap between its edge and the through hole 710 to allow gas to escape. After the pressure is released, the elastic sheet 720 quickly returns to its original position and reseals itself due to its own resilience. The elastic sheet 720 is made of butyl rubber or silicone material with a thickness of 0.3–0.5 mm, possessing excellent airtightness, low-temperature resistance, and fatigue life. It maintains its flexibility even at -196°C, ensuring it does not fail after repeated opening and closing. Furthermore, the one-piece injection molding process eliminates the risks of delamination, aging, or contamination that may result from adhesive bonding, improving the product's biocompatibility and long-term reliability.

[0037] The cryopreservation tube of this application achieves intelligent pressure adaptive regulation, effectively preventing tube burst accidents, while avoiding the backflow of external contaminants into the tube, significantly improving the safety and sealing stability of the cryopreservation tube under extreme temperature change conditions.

[0038] In one embodiment of this utility model, the radius of the chamfer 300 is 0.1mm-0.3mm.

[0039] In this embodiment, by setting a rounded chamfer 300 with a radius of 0.1mm–0.3mm on the inner edge of the cryopreservation tube opening, the technical problem of stress concentration, material cracking, or poor sealing caused by traditional right-angled or sharp-edge cryopreservation tubes in low-temperature environments is solved. Specifically, by machining a continuous and smooth concave arc transition on the inner edge of the tube opening facing the solution chamber 110, the rounded chamfer 300 structure can smoothly fit with the sealing ring 600 during assembly, avoiding micro-leakage caused by sharp edges scratching the sealing ring 600; at the same time, under low-temperature shrinkage, the arc structure can significantly reduce the local stress concentration coefficient, prevent the polypropylene material from generating micro-cracks due to embrittlement, thereby enhancing the impact resistance and fatigue resistance of the tube body 100.

[0040] In one embodiment of the present invention, along the axial extension direction of the tube body 100, the groove and the threaded groove 400 are arranged adjacent to each other, and the threaded groove 400 is disposed at one end of the tube opening near the bottom of the tube body 100.

[0041] In the embodiments of this application, the groove for installing the sealing ring 600 and the threaded groove 400 for connecting the cover 200 are arranged adjacent to each other along the axial direction of the tube body 100 on the outside of the tube opening. The threaded groove 400 is located at the end of the tube opening near the bottom of the tube body 100, meaning the threaded area is closer to the main body of the tube body 100, while the groove is located above it, near the top of the tube opening. This arrangement makes the compression sealing area of ​​the sealing ring 600 closer to the end face of the tube opening, shortening the force transmission path, improving the stability and uniformity of the sealing preload, and effectively preventing sealing failure due to improper assembly or vibration. Furthermore, the threaded engagement between the tube body 100 and the cover 200 enables the sealing ring 600 to be compressed and fixed, preventing loosening of the sealing ring 600 and improving the sealing stability of the cryopreservation tube.

[0042] In one embodiment of this utility model, the threads of the tube body 100 and the cover body 200 are fine-pitch trapezoidal threads with a pitch of 0.8mm-1.2mm and a height of 0.2mm-0.4mm, and the thread tip has a flattened structure.

[0043] The thread adopts a fine-pitch trapezoidal thread with a pitch controlled at 0.8–1.2 mm and a height of 0.2–0.4 mm. Compared with traditional triangular threads, the trapezoidal thread profile has a gentler sidewall angle and a shallower thread groove depth (400), resulting in tighter thread engagement, higher tensile strength, and less likelihood of gaps due to material shrinkage at low temperatures. Furthermore, the thread tip is designed with a flattened structure, meaning the thread tip is planar. When the cap 200 is tightened, the thread tip can make surface contact with the bottom of the corresponding thread groove (400), further compressing air and liquid in the thread gaps, expelling residual media, and preventing it from stagnating and forming a "dead volume" that could breed microorganisms or carry contaminants. This structural design is significantly necessary: ​​the fine-pitch design improves tightening accuracy and connection stability, suitable for the sealing needs of micro-volume, high-value samples; the trapezoidal thread profile reduces the capillary effect of liquid creeping along the thread, reducing the risk of sample residue and cross-contamination; and the flattened structure enhances the compaction effect at the sealing end, improving overall seal integrity.

[0044] Therefore, the cryopreservation tube of this application, through the synergistic optimization of thread layout and geometric parameters, not only improves the structural reliability of the cryopreservation tube under repeated freeze-thaw cycles and high pressure differential environments, but also reduces the risk of contamination from the source. It is particularly suitable for long-term low-temperature storage of biological samples with extremely high requirements for sealing and cleanliness.

[0045] In one embodiment of this utility model, the tube 100 is made of transparent polypropylene with a wall thickness of 0.8mm-1.2mm, a length of 55mm-65mm, and an outer diameter of 10mm-12mm.

[0046] The cryopreservation tube of this application uses transparent polypropylene as the tube body 100 material. This material has excellent low-temperature resistance, maintaining good toughness and dimensional stability at -196℃, avoiding brittleness and cracking. Its high transparency allows users to visually observe the sample state and liquid level. The wall thickness of the tube body 100 is controlled within the range of 0.8mm-1.2mm. This thickness ensures the tube body 100's resistance to deformation under internal and external pressure changes, preventing collapse or bursting due to excessive thinness, while avoiding material waste and reduced heat transfer efficiency due to excessive thickness, thus facilitating rapid freeze-thaw cycles.

[0047] The tube body 100 has a length of 55mm-65mm and an outer diameter of 10mm-12mm. This size range has been standardized and can be perfectly adapted to mainstream cryopreservation boxes, liquid nitrogen storage racks and automated sample management systems on the market, improving equipment compatibility and experimental throughput.

[0048] In one embodiment of this utility model, the outer wall of the tube 100 is provided with scale lines 800.

[0049] In the embodiments of this application, by setting precise graduation marks on the outer wall of the tube 100, the sample volume can be directly read without additional transfer or measurement, reducing operational steps and the risk of contamination. Specifically, the graduations typically extend upwards from the bottom of the tube, with a minimum division of 0.1 ml, meeting the needs for precise sample addition and verification of trace biological materials such as cell suspensions and tissue samples.

[0050] The above description is merely a preferred embodiment of this application and does not limit the patent scope of this application. Any equivalent structural transformations made based on the inventive concept of this application and the contents of the specification and drawings of this application, or direct / indirect applications in other related technical fields, are included within the patent protection scope of this application.

Claims

1. An explosion-proof cryopreservation tube, characterized in that, include: The tube body (100) and the cover (200) are provided. The tube body (100) is provided with a solution chamber (110) and a tube opening communicating with the solution chamber (110). The inner side of the tube opening facing the solution chamber (110) is provided with a recessed arc chamfer (300). The outer side of the tube opening is provided with a threaded groove (400) and a groove for installing a sealing ring (600). The threaded groove (400) and the groove are distributed along the axial direction of the tube body (100). The cover (200) is sealed to the tube body (100) through the threaded groove (400). The sealing ring (600) is sealed and abutted against the groove through the cover (200). The top of the cover (200) is provided with an exhaust valve (700). The exhaust valve (700) includes a through hole (710) on the top of the cover (200) and an elastic sheet (720) covering the through hole (710). The edge of the elastic sheet (720) is fixed to the top surface of the cover (200). When the pressure inside the cryopreservation tube increases, a portion of the elastic sheet (720) bulges up and forms an exhaust gap with the through hole (710). When the pressure inside the cryopreservation tube is balanced, the elastic sheet (720) springs back and seals the through hole (710).

2. The explosion-proof cryopreservation tube according to claim 1, characterized in that, Along the axial direction extending from the opening towards the bottom wall of the tube body (100), the diameter of the groove gradually decreases.

3. The explosion-proof cryopreservation tube according to claim 1, characterized in that, The cover (200) has an annular groove at the bottom of its inner side. The sealing ring (600) includes a first fixing part (610) and a second fixing part (620). The first fixing part (610) is installed in the annular groove. The second fixing part (620) is an annular convex structure. Along the direction away from the first fixing part (610) from the second fixing part (620), the thickness of the second fixing part (620) gradually decreases.

4. The explosion-proof cryopreservation tube according to claim 1, characterized in that, The ratio of the diameter of the through hole (710) to the diameter of the cover (200) is 1:10; The elastic sheet (720) is a circular thin sheet, the ratio of the diameter of the through hole (710) to the diameter of the elastic sheet (720) is 1:3, and the thickness of the elastic sheet (720) is 0.3mm-0.5mm.

5. The explosion-proof cryopreservation tube according to claim 1, characterized in that, The elastic sheet (720) is made of butyl rubber or silicone, and the elastic sheet (720) and the top surface of the cover (200) are integrally injection molded.

6. The explosion-proof cryopreservation tube according to claim 1, characterized in that, The radius of the circular chamfer (300) is 0.1mm-0.3mm.

7. The explosion-proof cryopreservation tube according to claim 1, characterized in that, Along the axial extension direction of the tube body (100), the groove is disposed adjacent to the threaded groove (400), and the threaded groove (400) is disposed at one end of the tube opening near the bottom of the tube body (100).

8. The explosion-proof cryopreservation tube according to claim 1, characterized in that, The threads of the tube body (100) and the cover body (200) are fine-tooth trapezoidal threads with a pitch of 0.8mm-1.2mm and a height of 0.2mm-0.4mm. The thread tip has a flattened structure.

9. The explosion-proof cryopreservation tube according to any one of claims 1-8, characterized in that, The tube (100) is made of transparent polypropylene with a wall thickness of 0.8mm-1.2mm, a length of 55mm-65mm, and an outer diameter of 10mm-12mm.

10. The explosion-proof cryopreservation tube according to any one of claims 1-8, characterized in that, The outer wall of the tube (100) is provided with scale lines (800).

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