A fluidized bed for phosphatidylserine production

Abstract

This invention belongs to the field of phosphatidylserine production technology, and specifically relates to a fluidized bed for phosphatidylserine production. It includes a fluidized bed body, a sealing gate, and a support assembly. The fluidized bed body has a feed pipe at the bottom and a discharge pipe at the top. A sealing gate is rotatably connected to the fluidized bed body. A support assembly is mounted on a support frame on the sealing gate. The support assembly consists of a support plate, a cover plate, and a sealing ring. A porous carrier for phospholipase is stored in the cavity formed by the combination of the support plate and the cover plate. The cover plate is fitted onto the support plate. In this invention, the phospholipase carrier is installed in the internal cavity of the fluidized bed through the cooperation of the support assembly and the support frame on the sealing gate. When replacing the phospholipase carrier, simply open the sealing gate and remove the support assembly from the fluidized bed. This facilitates quick disassembly and replacement of the support assembly, simplifying fluidized bed maintenance and repair, and improving work efficiency.

Description

Technical Field

[0001] This utility model relates to the field of phosphatidylserine production technology, specifically a fluidized bed for phosphatidylserine production. Background Technology

[0002] Phosphatidylserine is an important membrane phospholipid found in bacteria, yeast, plants, and mammalian cells. Also known as complex nervonic acid, it has the functions of improving nerve cell function, regulating nerve impulse transmission, and enhancing brain memory function, and has a wide range of applications.

[0003] In the production of phosphatidylserine, the substrate solution used for its production requires phospholipase catalysis to prepare phosphatidylserine. Therefore, a fluidized bed is needed to facilitate the catalysis of the raw materials by the phospholipase. However, the phospholipase is mounted inside the fluidized bed via a carrier, making it inconvenient to quickly replace and maintain after depletion, thus hindering its use. Utility Model Content

[0004] (a) Technical problems to be solved

[0005] To address the shortcomings of existing technologies, this invention provides a fluidized bed for the production of phosphatidylserine, which solves the problem that phosphatase, when installed inside the fluidized bed via a carrier, is inconvenient to replace and maintain quickly after depletion, and is therefore inconvenient to use.

[0006] (II) Technical Solution

[0007] To achieve the above objectives, this utility model specifically adopts the following technical solution:

[0008] A fluidized bed for the production of phosphatidylserine includes a fluidized bed body, a sealing gate, and a support assembly. The fluidized bed body has a feed pipe at the bottom and a discharge pipe at the top. A sealing gate is rotatably connected to the fluidized bed body. A support assembly is mounted on a support frame on the sealing gate. The support assembly consists of a support plate, a cover plate, and a sealing ring. A porous carrier for carrying phosphatidylserine is stored in the cavity formed by the combination of the support plate and the cover plate. The cover plate is fitted onto the support plate.

[0009] Furthermore, an annular frame is fixedly connected to the bearing plate, and an embedding groove is provided on the annular frame. The cover plate is fixedly embedded in the embedding groove through the embedding frame at the edge, and both the bearing plate and the cover plate are provided with evenly distributed perforations.

[0010] The bearing plate and the cover plate form a cavity for placing the porous carrier, and the carrier component formed by the combination of the bearing plate and the cover plate is sealed to the fluidized bed body and the sealing door through a sealing ring, which is fixedly fitted on the annular frame.

[0011] Furthermore, one side of the sealing door is rotatably connected to the fluidized bed body via a hinge, and the other side is detachably connected to the fluidized bed body via a locking screw. The sealing door is also configured to cooperate with the disassembly and assembly window opened on the fluidized bed body.

[0012] A sealing gasket is installed at the contact point between the sealing door and the disassembly window, and the sealing gasket is fixedly embedded in the installation groove opened at the corresponding position on the sealing door;

[0013] The mounting frames on the blocking gate are evenly spaced and form installation areas for the load-bearing components between each pair. The load-bearing components are slidably inserted into the installation areas formed between two adjacent mounting frames.

[0014] (III) Beneficial Effects

[0015] Compared with the prior art, this utility model provides a fluidized bed for the production of phosphatidylserine, which has the following beneficial effects:

[0016] In this invention, the phospholipase carrier is installed in the internal cavity of the fluidized bed through a carrier component and a mounting bracket on the sealing door. The carrier component can move together with the opening and closing of the sealing door. When replacing the phospholipase carrier, simply open the sealing door and remove the carrier component from the fluidized bed. This facilitates quick disassembly and replacement of the carrier component, making fluidized bed maintenance and repair easier and improving work efficiency. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model;

[0018] Figure 2 This is a cross-sectional view of the present invention;

[0019] Figure 3 This is a schematic diagram of the structure of the fluidized bed body in this utility model;

[0020] Figure 4 This is a schematic diagram of the blocking door in this utility model;

[0021] Figure 5 This is a cross-sectional view of the load-bearing component in this utility model;

[0022] Figure 6 This is an exploded view of the load-bearing component in this utility model.

[0023] In the diagram: 1. Fluidized bed body; 101. Feed pipe; 102. Discharge pipe; 103. Assembly / disassembly window; 2. Sealing door; 201. Frame; 3. Bearing component; 301. Bearing plate; 3011. Annular frame; 3012. Embedded sink; 302. Cover plate; 3021. Embedded frame; 303. Sealing ring; 4. Hinge; 5. Locking screw. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0025] Example

[0026] like Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 As shown in one embodiment of this utility model, a fluidized bed for the production of phosphatidylserine includes a fluidized bed body 1, a sealing gate 2, and a support assembly 3. The fluidized bed body 1 has a feed pipe 101 at the bottom and a discharge pipe 102 at the top, facilitating the smooth injection of raw materials into the fluidized bed and the discharge of products from the fluidized bed. The sealing gate 2 is rotatably connected to the fluidized bed body 1, and the support assembly 3 is mounted on a support frame 201 mounted on the sealing gate 2, using the sealing gate 2 as the frame for mounting the support assembly 3. This allows it to be stably installed in the internal cavity of the fluidized bed, ensuring stable use. At the same time, the support component 3 can move together with the sealing door 2, which facilitates the quick disassembly and replacement of the support component 3, improving the convenience of fluidized bed maintenance and repair, and improving work efficiency. The support component 3 is composed of a support plate 301, a cover plate 302 and a sealing ring 303. The cavity formed by the combination of the support plate 301 and the cover plate 302 contains a porous carrier carrying phospholipase. The cover plate 302 is embedded in the support plate 301.

[0027] In use, the conveying pipe for transporting the substrate solution for phosphatidylserine production is connected to the feed pipe 101, and then smoothly injected from the bottom into the internal cavity of the fluidized bed body 1. With continuous injection, the substrate solution for phosphatidylserine production passes through the support component 3, and when passing through, it effectively contacts the phospholipase on the porous support to carry out a catalytic reaction, catalyzing the synthesis of phosphatidylserine from phosphatidylcholine and ethanolamine. The generated phosphatidylserine is discharged from the upper discharge pipe 102 and smoothly transported away through the conveying pipe connected to the discharge pipe 102.

[0028] After the phospholipase on the porous carrier is consumed after a period of use, the sealing door 2 is opened directly, and the carrier component 3 is moved out of the fluidized bed body 1 using the sealing door 2. Then, the carrier component 3 is removed from the support frame 201, and the cover plate 302 is separated from the carrier plate 301. A new porous carrier is replaced, and the cover plate 302 is put back on to complete the replacement of the porous carrier. Finally, it can be reset and used again.

[0029] In this process, phospholipase is immobilized on a porous support through adsorption, embedding, or covalent bonding, forming solid particles with a diameter of 0.1-2 mm. When the substrate solution for phosphatidylserine production flows upward from the bottom of the fluidized bed through the porous support on the support component 3, the flow rate exceeds the critical fluidization velocity, causing the phospholipase particles on the porous support to suspend and randomly tumble. The fluid turbulence forces the substrate molecules to diffuse rapidly to the enzyme active site. At the same time, the product phosphatidylserine and the byproduct choline continuously detach from the particle surface and enter the fluid phase. Particle collisions and fluid shear forces destroy the stagnant layer at the liquid-solid interface, reducing substrate diffusion resistance. The reaction rate is increased by more than 50% compared to a batch stirred reactor. The substrate is continuously fed, and the reaction solution flows out from the top. The phospholipase particles are retained on the porous support for recycling. The reaction efficiency is indirectly adjusted by controlling the residence time of the substrate in the bed by regulating the fluid flow rate.

[0030] like Figure 2 , Figure 5 and Figure 6 As shown, in some embodiments, an annular frame 3011 is fixedly connected to the carrier plate 301, and an embedding groove 3012 is provided on the annular frame 3011. The cover plate 302 is fixedly embedded in the embedding groove 3012 through the embedding frame 3021 at the edge, so that the cover plate 302 can be stably installed on the carrier plate 301 by using the embedding frame 3021, so that the two can be stably matched to form a carrier, thereby installing the porous carrier and making it stable for use. Both the carrier plate 301 and the cover plate 302 are provided with uniformly distributed perforations, so that the substrate solution used for phosphatidylserine production can pass smoothly through the carrier and effectively contact the phospholipase on the porous carrier when passing through, and use the phospholipase to catalyze the synthesis of phosphatidylserine from phosphatidylcholine and ethanolamine.

[0031] A cavity for placing the porous carrier is formed between the carrier plate 301 and the cover plate 302, ensuring stable installation of the porous carrier. The carrier component formed by the combination of the carrier plate 301 and the cover plate 302 is sealed to the fluidized bed body 1 and the sealing door 2 through the sealing ring 303. The sealing ring 303 is fixedly fitted on the annular frame 3011. The sealing ring 303 seals the gap between the carrier component 3 and the fluidized bed body 1 and the sealing door 2, preventing the substrate solution used for phosphatidylserine production from seeping out of the gap and improving the contact efficiency between the substrate solution used for phosphatidylserine production and phospholipase.

[0032] like Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, in some embodiments, the sealing door 2 is rotatably connected to the fluidized bed body 1 on one side via a hinge 4, and detachably connected to the fluidized bed body 1 on the other side via a locking screw 5. This facilitates the stable installation of the sealing door 2 on the fluidized bed body 1 using the hinge 4, and ensures that the sealing door 2 can rotate smoothly on the fluidized bed body 1 during use, thereby achieving smooth opening and closing of the sealing door 2. At the same time, when the sealing door 2 is closed, the locking screw 5 fixes and limits the sealing door 2 to the fluidized bed body 1, so as to stably seal the disassembly window 103, facilitating... For stable use, to ensure that the sealing door 2 can be opened and closed smoothly, a handle can be installed on the sealing door 2 according to actual use needs to improve the convenience of operation. The sealing door 2 is designed in conjunction with the disassembly and assembly window 103 opened on the fluidized bed body 1 for installing the sealing door 2 and the load-bearing component 3 thereon. This allows the load-bearing component 3 to be stably installed in the internal cavity of the fluidized bed body 1, so that they can work together stably. At the same time, it is convenient to disassemble the load-bearing component 3 from the internal cavity of the fluidized bed body 1, so as to facilitate its quick replacement and improve the convenience of use.

[0033] Among them, the hinge 4 and the locking screw 5 are existing technologies, so they will not be described in detail.

[0034] A sealing gasket is installed at the contact point between the sealing door 2 and the disassembly window 103. The sealing gasket is fixedly embedded in the installation groove opened at the corresponding position on the sealing door 2, so as to make the sealing door 2 stably and securely connected to the disassembly window 103, thereby stably sealing the disassembly window 103, avoiding leakage during use, and ensuring stable use.

[0035] The mounting frames 201 on the sealing gate 2 are evenly distributed and form installation areas for the load-bearing components 3 between each pair. The load-bearing components 3 are slidably inserted into the installation areas formed between two adjacent mounting frames 201, which facilitates the stable installation of the load-bearing components 3 on the sealing gate 2, ensuring that the two cooperate with each other for stable use, and then the load-bearing components 3 are stably transferred to the internal cavity of the fluidized bed body 1 for use.

[0036] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A fluidized bed for the production of phosphatidylserine, comprising a fluidized bed body (1), a sealing gate (2), and a support assembly (3), characterized in that: The fluidized bed body (1) is provided with a feed pipe (101) at the bottom and a discharge pipe (102) at the top. A sealing door (2) is rotatably connected to the fluidized bed body (1). A support assembly (3) is placed on the support frame (201) on the sealing door (2). The support assembly (3) consists of a support plate (301), a cover plate (302) and a sealing ring (303). The cavity formed by the combination of the support plate (301) and the cover plate (302) contains a porous carrier carrying phospholipase. The cover plate (302) is fitted onto the support plate (301).

2. A fluidized bed for phosphatidylserine production according to claim 1, characterized in that: The support plate (301) is fixedly connected to an annular frame (3011), and an embedding groove (3012) is provided on the annular frame (3011). The cover plate (302) is fixedly embedded in the embedding groove (3012) through the embedding frame (3021) at the edge. Both the support plate (301) and the cover plate (302) are provided with evenly distributed perforations.

3. A fluidized bed for phosphatidylserine production according to claim 2, characterized in that: The bearing plate (301) and the cover plate (302) form a cavity for placing the porous carrier. The carrier formed by the combination of the bearing plate (301) and the cover plate (302) is sealed to the fluidized bed body (1) and the sealing door (2) through the sealing ring (303). The sealing ring (303) is fixedly fitted on the annular frame (3011).

4. A fluidized bed for phosphatidylserine production according to claim 1, characterized in that: The sealing door (2) is rotatably connected to the fluidized bed body (1) on one side by a hinge (4), and detachably connected to the fluidized bed body (1) on the other side by a locking screw (5). The sealing door (2) is configured in conjunction with the disassembly and assembly window (103) opened on the fluidized bed body (1).

5. A fluidized bed for the production of phosphatidylserine according to claim 4, characterized in that: A sealing gasket is installed at the contact point between the sealing door (2) and the disassembly window (103), and the sealing gasket is fixedly embedded in the mounting groove opened at the corresponding position on the sealing door (2).

6. A fluidized bed for the production of phosphatidylserine according to claim 1, characterized in that: The mounting frames (201) on the blocking door (2) are evenly distributed and form an installation area for the load-bearing component (3) between each pair. The load-bearing component (3) is slidably inserted into the installation area formed between two adjacent mounting frames (201).

Images