Gas supply device for DNA biosynthesis

Abstract

This application discloses a gas supply device for DNA biosynthesis, including a waste gas collection assembly and a housing. Inside the housing are a gas source supply assembly and a purge gas supply assembly. The gas source supply assembly consists of a first-stage pressure regulating valve, a first-second-stage pressure regulating valve, a first on / off solenoid valve, a flow divider, a check valve, and a vent valve connected in series. The first-stage pressure regulating valve is connected to a first-type gas source, and its output pressure does not exceed the upper limit of the first-second-stage pressure regulating valve, which has higher precision. The number of branches on the flow divider matches the number of reaction reagent types. The outlet of the check valve is connected to a reaction reagent bottle via a transition gas pipe, and the vent valve is installed in the transition gas pipe. The purge gas supply assembly consists of a second-stage pressure regulating valve, a second-second-stage pressure regulating valve, and a second on / off solenoid valve connected in series, connected to a second-type gas source, also with two-stage pressure regulation. The waste gas collection assembly is connected to the vent valve for collecting waste gas. This device can provide a stable gas supply and precise waste gas treatment for DNA biosynthesis.

Description

Technical Field

[0001] This disclosure relates to the field of DNA biosynthesis technology, and more particularly to a gas supply device for DNA biosynthesis. Background Technology

[0002] In the field of DNA synthesis, chemical synthesis technologies mainly fall into two generations. The first generation is column synthesis, and the second generation is high-throughput chip synthesis based on inkjet printing technology. DNA synthesis primarily involves four chemical reactions: coupling, capping, oxidation, and deprotection. In the first generation, the flow rate of chemical reagents was precisely controlled by adjusting the opening and closing times of solenoid valves. In the second generation, reagents for the coupling reaction are supplied by the printhead, and the flow rate of reagents for the other three reactions is also controlled by solenoid valves. The key factor in controlling the flow rate of reagents by opening and closing the solenoid valves is gas pressure, which is the driving force for reagent flow. Because high-throughput chip synthesis involves smaller and more numerous reaction sites, precise control of the flow rate is even more crucial for the second-generation synthesizer.

[0003] For reagent bottle gas supply, precise and stable pressure control is crucial for the synthesis reaction. First, unstable pressure will cause variations in the influent volume, leading to decreased reaction efficiency or contamination due to incomplete cleaning. In long-chain synthesis, the adverse effects of unstable pressure are more pronounced as coupling efficiency gradually decreases; therefore, pressure control must remain consistently stable throughout the entire synthesis process. Second, leaks can introduce air or moisture into the reagents. DNA synthesis is highly sensitive to water and oxygen, especially moisture; a water content exceeding 1 ppm typically causes a significant drop in reaction efficiency. Although all reagents are protected with argon gas and molecular sieves are added to the cleaning reagents to remove water, leaks in the gas supply piping, connectors, and all gas path components can still have a significant impact on the synthesis reaction.

[0004] Besides pressure and leakage issues, reagent vapor crosstalk and incompatibility between gas path materials and reagents can also adversely affect DNA synthesis reactions. If reagent vapor crosstalk exists, it can degrade the performance of some reagents; for example, oxidant vapor crossing into the deprotecting agent can reduce its performance, leading to poorer synthesis results. Incompatibility between gas path materials and reagents can cause leaks, crosstalk, and the introduction of impurities, all of which hinder the smooth progress of DNA synthesis reactions. Utility Model Content

[0005] In view of this, the present disclosure provides a gas supply device for DNA biosynthesis, which at least partially solves the problems of unstable gas pressure and poor air tightness of gas supply devices in the prior art.

[0006] This disclosure provides a gas supply device for DNA biosynthesis, comprising: The housing; The housing is equipped with a gas supply assembly for driving reagent flow and a purging gas supply assembly. The gas supply assembly includes a first-stage pressure regulating valve, a first-second-stage pressure regulating valve, a first on / off solenoid valve, a flow divider, a one-way valve, and a vent valve connected in series. The inlet of the first-stage pressure regulating valve is connected to a first type of gas source, and the output pressure of the first-stage pressure regulating valve is not higher than the upper pressure threshold of the first-second-stage pressure regulating valve. The accuracy of the first-second-stage pressure regulating valve is greater than that of the first-stage pressure regulating valve. The number of branch paths of the flow divider is consistent with the number of reaction reagent types. The outlet of the one-way valve is connected to the reaction reagent bottle through a transition gas pipe, and the vent valve is installed in the transition gas pipe. The purging gas supply assembly includes a second-stage pressure regulating valve, a second-second-stage pressure regulating valve, and a second on / off solenoid valve connected in series. The inlet of the second-stage pressure regulating valve is connected to a second type of gas source, and the output pressure of the second-stage pressure regulating valve is not higher than the upper limit threshold of the pressure of the second-second-stage pressure regulating valve. The accuracy of the second-second-stage pressure regulating valve is greater than that of the second-stage pressure regulating valve. The exhaust gas collection assembly is connected to the vent valve.

[0007] The gas supply device for DNA biosynthesis provided in this embodiment provides a gas source supply component, a purge gas supply component, and a waste gas collection component within a housing. The gas source supply component provides driving gas to the reaction reagent bottles through multi-stage pressure regulation, on / off control, and diversion. The purge gas supply component provides purge gas through multi-stage pressure regulation and on / off control. The waste gas collection component collects the waste gas discharged from the vent valve, thereby achieving the purpose of providing stable driving gas and purge gas for DNA biosynthesis and treating waste gas, ensuring the smooth progress of the DNA biosynthesis process.

[0008] The above description is merely an overview of the technical solution disclosed herein. In order to better understand the technical means of this disclosure and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this disclosure more apparent and understandable, preferred embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0009] To more clearly illustrate the technical solutions of the embodiments of this disclosure, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this disclosure. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0010] Figure 1 This is a schematic diagram of a gas supply device for DNA biosynthesis provided in an embodiment of this disclosure.

[0011] Figure 2 for Figure 1 The diagram shows the first gas supply assembly, the second gas supply assembly, and the purging gas supply assembly.

[0012] Figure 3 for Figure 2 A schematic diagram of the first gas supply component in the diagram.

[0013] Figure 4 for Figure 2 A schematic diagram of the purging gas supply assembly.

[0014] Figure 5 for Figure 1 A schematic diagram of the exhaust gas collection component.

[0015] Explanation of reference numerals in the attached figures: 100. Housing; 200. First gas supply assembly; 210. First stage pressure regulating valve; 220. First and second stage pressure regulating valves; 230. First on / off solenoid valve; 240. Diverter plate; 250. Check valve; 260. Relief valve; 270. First through-plate compression fitting; 280. First digital pressure gauge; 291. First compression fitting; 292. First male threaded fitting; 293. Second male threaded fitting; 294. First gas pipe; 300. Purge gas supply assembly; 310. Second stage pressure regulating valve; 3 20. Second and second stage pressure regulating valves; 330. Second on / off solenoid valve; 340. Second air pipe; 350. Second through-plate compression fitting; 360. Second digital pressure gauge; 371. Second compression fitting; 372. Third male threaded fitting; 373. Fourth male threaded fitting; 374. Compression fitting threaded tee; 400. Second set of air supply components; 500. Waste gas collection components; 510. Waste collection plate; 520. Exhaust pipe; 530. Third snap-fit ​​sleeve; 540. Pagoda fitting; 550. Fourth snap-fit ​​sleeve. Detailed Implementation

[0016] The present disclosure will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for illustrative purposes only and are not intended to limit the scope of the disclosure. Furthermore, it should be noted that, for ease of description, only the parts relevant to the present disclosure are shown in the accompanying drawings.

[0017] It should be noted that, where there is no conflict, the embodiments and features described in this disclosure can be combined with each other. The technical solutions of this disclosure will now be described in detail with reference to the accompanying drawings and embodiments.

[0018] Unless otherwise stated, the exemplary implementations / embodiments shown are to be understood as providing exemplary features of various details that provide ways in which the technical concepts of this disclosure can be implemented in practice. Therefore, unless otherwise stated, the features of various implementations / embodiments may be additionally combined, separated, interchanged and / or rearranged without departing from the technical concepts of this disclosure.

[0019] The use of crosshairs and / or shading in the accompanying drawings is generally used to clarify the boundaries between adjacent components. Thus, unless otherwise stated, the presence or absence of crosshairs or shading does not convey or indicate any preference or requirement for the specific material, material properties, dimensions, proportions, commonalities between the illustrated components, or any other characteristics, properties, etc., of the components. Furthermore, in the accompanying drawings, the dimensions and relative dimensions of components may be exaggerated for clarity and / or descriptive purposes. When exemplary embodiments can be implemented differently, a specific process sequence may be performed in a different order than that described. For example, two consecutively described processes may be performed substantially simultaneously or in the reverse order of their description. Furthermore, the same reference numerals denote the same components.

[0020] When a component is referred to as being "on" or "above" another component, "connected to," or "joined to" another component, the component may be directly on, directly connected to, or directly joined to the other component, or there may be intermediate components. However, when a component is referred to as being "directly on" another component, "directly connected to," or "directly joined to" another component, there are no intermediate components. Therefore, the term "connection" can refer to a physical connection, an electrical connection, etc., and may or may not have intermediate components.

[0021] For descriptive purposes, this disclosure may use spatial relative terms such as “below,” “under,” “below,” “down,” “above,” “above,” “higher,” and “side (e.g., in a “sidewall”)” to describe the relationship between one component and another component as shown in the accompanying drawings. In addition to the orientations depicted in the drawings, the spatial relative terms are also intended to encompass different orientations of the device during use, operation, and / or manufacture. For example, if the device in the drawings is flipped, a component described as “below” or “under” another component or feature would subsequently be positioned “above” said other component or feature. Thus, the exemplary term “below” can encompass both “above” and “below” orientations. Furthermore, the device may be otherwise positioned (e.g., rotated 90 degrees or in other orientations), thus interpreting the spatial relative descriptive terms used herein accordingly.

[0022] The terminology used herein is for the purpose of describing particular embodiments and is not intended to be limiting. As used herein, unless the context clearly indicates otherwise, the singular forms “a” and “the” are intended to include the plural forms as well. Furthermore, when the terms “comprising” and / or “including” and variations thereof are used in this specification, it indicates the presence of the stated features, integrals, steps, operations, parts, components, and / or groups thereof, but does not exclude the presence or addition of one or more other features, integrals, steps, operations, parts, components, and / or groups thereof. It should also be noted that, as used herein, the terms “substantially,” “about,” and other similar terms are used as approximate terms rather than as terms of degree, thus explaining the inherent biases in measurements, calculated values, and / or provided values ​​that would be recognized by one of ordinary skill in the art.

[0023] Reference Figure 1 and Figure 2 This application discloses a gas supply device for DNA biosynthesis, comprising: a housing 100, a gas supply component, a purge gas supply component 300, a waste gas collection component 500, a first type of gas source and a second type of gas source. The gas supply component for driving reagent flow and the purge gas supply component 300 are installed inside the housing 100. The purge gas supply component 300 is used for switching control of the purge gas. Specifically, the gas supply components are set in two groups. The first group of gas supply components 200 is used to drive the flow of waterless reagents, and the second group of gas supply components 400 is used to drive the flow of water-containing reagents. That is, the two groups supply gas to reagents containing water and waterless reagents respectively, which can prevent vapor cross-contamination.

[0024] The second gas supply component 400 is configured in the same way as the first gas supply component 200.

[0025] The preferred gas source for the first type is argon, because argon has a high density and can be deposited in the reagent bottle to protect the reagent even without pressure. It is also relatively less soluble in the reagent, so the reaction reagent will not precipitate bubbles and will not affect the reaction area. The preferred gas source for the second type is nitrogen, which ensures that the pipeline is clean and easy to purge.

[0026] The housing 100 is preferably a hollow cuboid structure, which provides housing protection for the main components of the gas supply assembly and the purging gas supply assembly 300, as well as a fixed assembly of the whole.

[0027] Reference Figure 3 Taking the first gas supply component 200 as an example, a detailed description of its structure will be provided.

[0028] The first gas supply assembly 200 includes a first-stage pressure regulating valve 210, a first-second-stage pressure regulating valve 220, a first on / off solenoid valve 230, a flow divider 240, a one-way valve 250, and a relief valve 260 connected in series. The inlet end of the first-stage pressure regulating valve 210 is connected to a first type of gas source, and the output gas pressure of the first-stage pressure regulating valve 210 is not higher than the upper limit threshold of the gas pressure of the first-second-stage pressure regulating valve 220, which is used to protect the internal components of the first-second-stage pressure regulating valve 220.

[0029] Among them, the accuracy of the first and second stage pressure regulating valves 220 is greater than that of the first stage pressure regulating valve 210.

[0030] The first on / off solenoid valve 230 is preferably in the normally open state, continuously supplying gas to deal with possible gas leakage problems and ensure continuous argon protection for the reagent bottle.

[0031] The number of branch paths of the flow divider 240 is consistent with the number of reaction reagent types. In this embodiment, the number of branch paths is preferably three groups, which supply gas to different reagents respectively. This can accurately divide the gas output from the gas supply component into different types of reaction reagent bottles, meeting the needs of DNA synthesis involving multiple reaction reagents. Each reaction reagent bottle can independently obtain a stable gas pressure to drive the reagent flow, ensuring that the liquid inlet volume of different reagents can be accurately controlled, thus improving the accuracy and efficiency of the DNA synthesis reaction.

[0032] The outlet of the one-way valve 250 is connected to the reagent bottle through a transition gas pipe, and the vent valve 260 is installed in the transition gas pipe.

[0033] Furthermore, a first pressure monitoring device is installed on the diverter plate 240, which is connected to the diverter branch respectively. The first pressure monitoring device is preferably a first digital display pressure gauge 280, which is used for air tightness detection of the gas circuit and the downstream liquid circuit. Through the corresponding program, the pressure holding test of the gas supply circuit and the downstream liquid circuit can be performed to determine whether there is leakage in the gas circuit and the downstream liquid circuit. The electronic pressure gauge can be set with a pressure drop alarm value, and the software can actively determine the leakage situation.

[0034] Each branch line is equipped with a one-way valve 250, which is installed in the gas path between the distributor plate 240 and the reagent bottle. This valve allows gas to flow only from the distributor plate 240 to the reagent bottle, preventing backflow of reagents and avoiding vapor cross-contamination between different reagents. The preferred opening pressure of the one-way valve 250 is 2 kPa; this extremely low opening pressure also ensures stable gas supply.

[0035] The one-way valve 250 and the downstream components are all made of corrosion-resistant materials. Each branch line is equipped with an independent relief valve 260 to release the pressure of the downstream reagent bottle. This is usually used when changing the reagent bottle to prevent the vapor inside the reagent bottle from leaking into the room and causing harm to the human body. The exhaust gas is centrally discharged to a safe area through the exhaust gas emission structure.

[0036] The output pressure of the first-stage pressure regulating valve 210 is P1, and the target gas source pressure for DNA biosynthesis is P0, where P1 - P0 > 5 kPa; the output pressure of the first-stage pressure regulating valve 220 is P2. .

[0037] The inlet of the first-stage pressure regulating valve 210 is connected to the first type of gas source via the first compression fitting 291; the first-stage pressure regulating valve 210 and the first-stage pressure regulating valve 220 are connected via the first external thread fitting 292, and the first-stage pressure regulating valve 220 is connected to the first on / off solenoid valve 230 via the second external thread fitting 293. The first on / off solenoid valve 230 is connected to the flow divider plate 240 via the first gas pipe 294; the transition gas pipe is connected to the reagent bottle via the first through-plate compression fitting 270.

[0038] Furthermore, when an airtightness test is required, the software program can control the normally open solenoid valve to close, and then determine whether there is a leak by detecting whether the pressure on the first digital pressure gauge 280 drops to the alarm value.

[0039] Reference Figure 4 The purging gas supply assembly 300 includes a second-stage pressure regulating valve 310, a second-stage pressure regulating valve 320, and a second on / off solenoid valve 330 connected in series. The inlet end of the second-stage pressure regulating valve 310 is connected to a second type of gas source, and the output pressure of the second-stage pressure regulating valve 310 is not higher than the upper limit threshold of the pressure of the second-stage pressure regulating valve 320. The accuracy of the second-stage pressure regulating valve 320 is greater than that of the second-stage pressure regulating valve 310.

[0040] Specifically, the output pressure of the second-stage pressure regulating valve 310 is P3, where P3-P0 > 5 kPa; the output pressure of the second-stage pressure regulating valve 320 is P4. .

[0041] The inlet of the second-stage pressure regulating valve 310 is connected to the second type of gas source through the second compression fitting 371; the second-stage pressure regulating valve 310 and the second-stage pressure regulating valve 320 are connected through the third external thread fitting 372, and the second-stage pressure regulating valve 320 is connected to the second on / off solenoid valve 330 through the fourth external thread fitting 373; the second on / off solenoid valve 330 is connected to the cleaning reagent bottle through the second gas pipe 340 and the second through-plate compression fitting 350.

[0042] The first-stage pressure regulating valve 210 and the second-stage pressure regulating valve 310 are preferably mechanical pressure regulating valves; the first-stage pressure regulating valve 220 and the second-stage pressure regulating valve 320 are preferably electronic proportional valves. The electronic proportional valve is a precision pneumatic pressure control element. The required pressure can be set by software, and the pressure control accuracy is ±1 kPa. It also has its own pneumatic pressure feedback, which can monitor the pressure at the outlet of the electronic proportional valve in real time. In case of abnormality, it can perform alarm, record, and interlock protection actions.

[0043] A second pressure monitoring device is installed on the second air pipe 340 and is connected to the second air pipe 340. The second pressure monitoring device is fixed to the second air pipe 340 through a compression fitting threaded tee 374. The second pressure monitoring device is preferably a second digital display pressure gauge 360, which is used for air tightness detection of the air circuit and the downstream liquid circuit. It can perform pressure holding tests on the air supply circuit and the downstream liquid circuit through corresponding programs to determine whether there is leakage in the air circuit and the downstream liquid circuit. The electronic pressure gauge can be set with a pressure drop alarm value, and the software can actively determine the leakage situation.

[0044] The second on / off solenoid valve 330 is preferably a normally closed solenoid valve. During DNA biosynthesis, it is normally closed. After the synthesis is completed, the valve is opened when purge gas is needed.

[0045] Furthermore, when an airtightness test is required, the software can determine whether a leak exists by detecting whether the pressure on the second digital pressure gauge 360 ​​drops to the alarm value.

[0046] In this application, the primary pressure regulating valve is used to provide a stable initial pressure source to ensure initial pressure stabilization; the secondary pressure regulating valve is used to precisely control the output pressure to meet the synthesis requirements.

[0047] The gas supply device for DNA biosynthesis disclosed in this application effectively ensures precise and stable control of gas pressure through two-stage pressure regulation. Both the gas source supply component and the purge gas supply component 300 employ a primary pressure regulating valve and a secondary pressure regulating valve connected in series. The primary pressure regulating valve initially adjusts the input gas pressure, ensuring that the output gas pressure does not exceed the upper pressure threshold of the secondary pressure regulating valve, providing a suitable pressure basis for secondary pressure regulation. The secondary pressure regulating valve has a higher precision than the primary valve, enabling further fine-tuning of the gas pressure to ensure precise and stable output pressure to the reaction reagent bottle or purge stage. This effectively avoids differences in liquid intake caused by unstable gas pressure, thereby preventing decreased reaction efficiency and contamination problems caused by incomplete cleaning. Especially in the synthesis of long-chain DNA, it ensures continuous and stable gas pressure throughout the synthesis process, reducing the adverse effects of unstable gas pressure on the synthesis reaction.

[0048] Reference Figure 1 and Figure 5The exhaust gas collection component 500 is connected to the relief valve 260 to collect the exhaust gas released by the relief valve 260 and discharge it to a designated safe area. During DNA synthesis, some harmful or polluting exhaust gases may be generated. The exhaust gas collection component 500 can centrally treat these exhaust gases to prevent them from being directly discharged into the environment and causing pollution, which meets environmental protection requirements and also provides a safe working environment for operators.

[0049] Specifically, the waste gas collection assembly 500 includes a waste collection plate 510 and an exhaust pipe 520; the waste collection plate 510 has a main hole and several sub-holes connected to the main hole, and the inlet of each sub-hole is connected to the vent valve 260 on a single branch line through a pagoda connector 540; the outlet of the main hole is connected to the exhaust pipe 520 through a third snap-fit ​​sleeve 530; the outlet of the exhaust pipe 520 is connected to the exhaust duct through a fourth snap-fit ​​sleeve 550, and is discharged outdoors after being filtered in the exhaust duct.

[0050] Furthermore, the ferrule seal in this application can effectively ensure that the connection of the air tube is firm and leak-free; all joints are preferably 316L stainless steel joints, and the air tubes are all Teflon air tubes. 316L and Teflon can effectively prevent moisture in the environment from seeping into the pipe through the pipe wall. The ferrule joint makes the connection of the air tube very firm and tight, and will not loosen or leak.

[0051] In the description of this specification, the references to terms such as "one embodiment / mode," "some embodiments / modes," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment / mode or example is included in at least one embodiment / mode or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment / mode or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments / modes or examples. Furthermore, without contradiction, those skilled in the art can combine and integrate the different embodiments / modes or examples described in this specification, as well as the features of different embodiments / modes or examples.

[0052] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0053] Those skilled in the art should understand that the above embodiments are merely for illustrating the present disclosure and are not intended to limit the scope of the disclosure. Those skilled in the art can make other changes or modifications based on the above disclosure, and these changes or modifications still fall within the scope of the present disclosure.

Claims

1. A gas supply device for DNA biosynthesis, characterized in that, include: The housing; The housing is equipped with a gas supply assembly for driving reagent flow and a purging gas supply assembly. The gas supply assembly includes a first-stage pressure regulating valve, a first-second-stage pressure regulating valve, a first on / off solenoid valve, a flow divider, a one-way valve, and a vent valve connected in series. The inlet of the first-stage pressure regulating valve is connected to a first type of gas source, and the output pressure of the first-stage pressure regulating valve is not higher than the upper pressure threshold of the first-second-stage pressure regulating valve. The accuracy of the first-second-stage pressure regulating valve is greater than that of the first-stage pressure regulating valve. The number of branch paths of the flow divider is consistent with the number of reaction reagent types. The outlet of the one-way valve is connected to the reaction reagent bottle through a transition gas pipe, and the vent valve is installed in the transition gas pipe. The purging gas supply assembly includes a second-stage pressure regulating valve, a second-second-stage pressure regulating valve, and a second on / off solenoid valve connected in series. The inlet of the second-stage pressure regulating valve is connected to a second type of gas source, and the output pressure of the second-stage pressure regulating valve is not higher than the upper limit threshold of the pressure of the second-second-stage pressure regulating valve. The accuracy of the second-second-stage pressure regulating valve is greater than that of the second-stage pressure regulating valve. The exhaust gas collection assembly is connected to the vent valve.

2. The gas supply device for DNA biosynthesis according to claim 1, characterized in that, The output pressure of the first stage pressure regulating valve is P1, the target gas source pressure for DNA biosynthesis is P0, and P1-P0>5kPa; The output pressure of the first secondary pressure regulating valve is P2. ; The output pressure of the second-stage pressure regulating valve is P3, where P3-P0 > 5 kPa; The output pressure of the second and second stage pressure regulating valve is P4. .

3. The gas supply device for DNA biosynthesis according to claim 1, characterized in that, The inlet of the first-stage pressure regulating valve is connected to the first type of gas source through the first compression fitting; The first-stage pressure regulating valve and the first-second-stage pressure regulating valve, as well as the first-second-stage pressure regulating valve and the first on / off solenoid valve, are all connected by external threaded connectors. The first on / off solenoid valve is connected to the flow divider plate via a first air pipe; The transition gas tube is connected to the reagent bottle via a first through-plate clamp connector.

4. The gas supply device for DNA biosynthesis according to claim 3, characterized in that, The inlet of the second-stage pressure regulating valve is connected to the second type of gas source through the second compression fitting; The second-stage pressure regulating valve and the second-second stage pressure regulating valve, as well as the second-second stage pressure regulating valve and the second on / off solenoid valve, are all connected by external threaded connectors. The second on / off solenoid valve is connected to the cleaning reagent bottle through the second air pipe and the second through-plate clamp connector.

5. The gas supply device for DNA biosynthesis according to claim 4, characterized in that, Both the first-stage pressure regulating valve and the second-stage pressure regulating valve are mechanical pressure regulating valves; Both the first and second secondary pressure regulating valves are electronic proportional valves.

6. The gas supply device for DNA biosynthesis according to claim 4, characterized in that, The first type of gas source is argon; The second type of gas source is nitrogen.

7. The gas supply device for DNA biosynthesis according to claim 4, characterized in that, The first pressure monitoring device, which is connected to the branch flow path, is installed on the flow divider plate. A second pressure monitoring device is provided on the second air tube and is connected to the second air tube. The second pressure monitoring device is fixed to the second air tube by a ferrule threaded tee.

8. The gas supply device for DNA biosynthesis according to claim 1, characterized in that, The gas supply components are provided in two sets. The first set of gas supply components is used to drive the flow of waterless reagents. The second set of gas supply components is used to drive the flow of water-containing reagents.

9. The gas supply device for DNA biosynthesis according to claim 1, characterized in that, Each branch line is equipped with a check valve; Each branch line is equipped with an independent vent valve.

10. The gas supply device for DNA biosynthesis according to claim 9, characterized in that, The waste gas collection assembly includes a waste collection plate and an exhaust pipe; The waste collection plate has a main hole and several sub-holes connected to the main hole. The inlet of each sub-hole is connected to a discharge valve on a single branch line through a pagoda connector. The outlet of the main hole is connected to the exhaust pipe through a third snap-fit ​​sleeve; the outlet of the exhaust pipe is connected to the exhaust duct through a fourth snap-fit ​​sleeve.

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