A centrifugal processing device for preparing stem cell gels

Abstract

This utility model discloses a centrifugal processing device for preparing stem cell gels, including a centrifugal processing mechanism disposed inside the main unit housing. The centrifugal processing mechanism consists of a sheath, a centrifugal chamber, and a centrifugal rotor. The centrifugal chamber is disposed at the top of the main unit housing, the sheath is disposed at the top of the main unit housing, and the centrifugal rotor is disposed inside the centrifugal chamber. The inner side of the centrifugal rotor is provided with multiple rotor hole assemblies. The centrifugal bushing is designed to be installed by a threaded method, which can effectively solve the drawbacks of bolt penetration and direct clamping. No special tools are required for installation; installation and disassembly can be completed by manual rotation, eliminating the tedious steps of unscrewing and loosening bolts in stages. This greatly improves the operational efficiency when frequently changing bushings. The threaded helical locking structure provides strong radial and axial fixation, making it difficult to loosen or shift under the huge centrifugal force generated by high-speed centrifugation, avoiding eccentric shaking of the centrifugal tubes, and ensuring stability.

Description

Technical Field

[0001] This utility model belongs to the field of biomedical engineering technology, specifically relating to a centrifugal processing device for preparing stem cell gels. Background Technology

[0002] The stem cell gel preparation centrifugation device is a specialized piece of equipment in the field of biomedical engineering. It is a core centrifugal component in the stem cell gel preparation process, using centrifugal force to separate, concentrate, and purify stem cells from impurities, removing culture medium, dead cells, and other contaminants. Simultaneously, it precisely controls the rotation speed, time, and temperature to ensure stem cell viability, providing high-purity, high-concentration stem cell raw materials for gel preparation. The device consists of a main unit, centrifuge rotor, temperature control system, and specialized centrifuge containers, and is widely used in regenerative medicine, biopharmaceuticals, and cell biology. It is a key piece of equipment for stem cell gel research, production, and preclinical studies.

[0003] Existing centrifuge processing devices use bolt-through installation for the internal bushings, which is cumbersome to operate. Installation requires special tools and specific steps to tighten the bolts in order to ensure even force distribution. Disassembly also requires loosening the bolts one by one, which is extremely inefficient, especially when frequently changing bushings of different sizes to fit centrifuge tubes. In addition, the direct snap-fit ​​installation of the bushings is not stable enough. The huge centrifugal force generated by the high-speed rotation of the centrifuge processing device during operation can easily cause the snap-fit ​​structure to loosen, shift, or even fall off, causing the centrifuge tubes to wobble off-center. To address this issue, this utility model proposes a centrifuge processing device for the preparation of stem cell gels. Utility Model Content

[0004] The purpose of this invention is to provide a centrifugation device for preparing stem cell gels, so as to solve the problem of inconvenient installation of the internal liner of the centrifugation device mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a centrifugal processing device for preparing stem cell gels, comprising a centrifugal processing mechanism disposed inside the main unit housing. The centrifugal processing mechanism consists of a protective sleeve, a centrifugal chamber, and a centrifugal rotor. The centrifugal chamber is located at the top of the main unit housing, providing space for centrifugal operation. The rotor rotates at high speed within the chamber, generating a centrifugal force field, which is the core area for the separation and concentration of stem cell suspensions. Simultaneously, a suitable temperature within the chamber can be maintained by a temperature control system. The protective sleeve is located at the top of the main unit housing, protecting the edge of the centrifugal chamber opening, reducing wear, and assisting in centrifugal processing. To enhance the airtightness of the centrifuge chamber and optimize the centrifugation environment, the centrifuge rotor is disposed inside the centrifuge chamber. Multiple rotor hole assemblies are provided on the inner side of the centrifuge rotor. Each rotor hole assembly consists of a centrifuge bushing, a silicone seat, a mounting component, and a rotor hole. The rotor hole is located at the top of the centrifuge chamber. The centrifuge bushing is disposed inside the rotor hole and nested within it, adapting to centrifuge tubes of different diameters to ensure the centrifuge tubes remain stable and do not wobble during high-speed rotation. The mounting component is located at the connection between the centrifuge bushing and the rotor hole, and the silicone seat is located at the connection between the bottom end of the centrifuge bushing and the bottom end of the rotor hole.

[0006] Preferably, the top of the centrifuge bushing is higher than the top of the centrifuge chamber.

[0007] Preferably, the installation assembly consists of a fixed sleeve, a movable sleeve, and symmetrically arranged limiting components. The fixed sleeve is embedded in the surface of the centrifugal bushing, the movable sleeve is embedded in the inner side of the rotor hole, and the symmetrically arranged limiting components are located at the connection between the movable sleeve and the inner side of the rotor hole.

[0008] Preferably, the fixing sleeve is a hollow cylindrical structure, and the outer surface of the fixing sleeve is threaded.

[0009] Preferably, the inner wall of the movable sleeve is threaded, and the inner wall of the movable sleeve is threadedly connected to the outer surface of the fixed sleeve.

[0010] Preferably, the limiting component consists of a limiting rotating block and a limiting rotating groove. The limiting rotating groove is formed on the inner wall of the rotor hole, and the limiting rotating block is sleeved and fixed on the surface of the movable sleeve. The limiting rotating block and the limiting rotating groove are in an engaged state.

[0011] Preferably, the top of the main unit housing is provided with a top cover, which enables the opening and closing of the centrifuge chamber. When closed, it forms a sealed space to prevent sample splashing during centrifugation and ensure operational safety. At the same time, in conjunction with the sealing design, it maintains the temperature and sterile environment inside the centrifuge chamber.

[0012] Compared with the prior art, the beneficial effects of this utility model are: The centrifuge bushings are designed with a threaded installation method, which effectively solves the drawbacks of bolt penetration and direct clamping. No special tools are required for installation; installation and disassembly can be completed by manual rotation, eliminating the tedious steps of unscrewing bolts in stages. This significantly improves the operational efficiency when frequently changing bushings. The threaded helical locking structure provides strong radial and axial fixation, making it less prone to loosening or displacement under the enormous centrifugal force generated by high-speed centrifugation. This prevents centrifuge tubes from eccentrically shaking and ensures stability. In addition, the tight thread fit and small gaps reduce the accumulation of culture medium residue and impurities, facilitating cleaning and sterilization. It is also more suitable for the aseptic requirements of stem cell gel preparation, balancing operational convenience and equipment reliability. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a schematic diagram of the top structure of the centrifugal processing device of this utility model; Figure 3 This utility model Figure 2 Enlarged schematic diagram of region A in the image; Figure 4 This is a cross-sectional schematic diagram of the rotor hole assembly of this utility model; In the diagram: 1. Main unit housing; 2. Top cover; 3. Sheath; 4. Centrifuge chamber; 5. Centrifuge rotor; 51. Centrifuge bushing; 52. Silicone seat; 53. Mounting assembly; 531. Fixed sleeve; 532. Movable sleeve; 533. Limiting assembly; 5331. Limiting rotating block; 5332. Limiting rotating groove; 54. Rotor hole. Detailed Implementation

[0014] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0015] Please see Figures 1 to 4This utility model provides a technical solution: a centrifugal processing device for preparing stem cell gel, including a centrifugal processing mechanism disposed inside the main unit housing 1. The centrifugal processing mechanism consists of a protective sleeve 3, a centrifugal chamber 4, and a centrifugal rotor 5. The centrifugal chamber 4 is disposed at the top of the main unit housing 1, the protective sleeve 3 is disposed at the top of the main unit housing 1, and the centrifugal rotor 5 is disposed inside the centrifugal chamber 4. Multiple rotor hole assemblies are disposed on the inner side of the centrifugal rotor 5. Each rotor hole assembly consists of a centrifugal bushing 51, a silicone seat 52, a mounting component 53, and rotor holes 54. The rotor holes 54 are opened at the top of the centrifugal chamber 4, the centrifugal bushing 51 is disposed inside the rotor holes 54, the mounting component 53 is disposed at the connection between the centrifugal bushing 51 and the rotor holes 54, and the silicone seat 52... 2. Located at the connection between the bottom end of the centrifuge bushing 51 and the bottom end of the rotor hole 54, the silicone seat 52 further reinforces the contact point between the bottom end of the centrifuge bushing 51 and the rotor hole 54. The centrifuge bushing 51, designed for threaded installation, effectively solves the drawbacks of bolt penetration and direct clamping. No special tools are required during installation; installation and disassembly can be completed by manual rotation, eliminating the tedious steps of unscrewing bolts and significantly improving operational efficiency when frequently changing bushings. The threaded helical locking structure provides strong radial and axial fixation, preventing loosening and displacement under the enormous centrifugal force generated by high-speed centrifugation, avoiding eccentric shaking of the centrifuge tubes, ensuring stability. Furthermore, the tight thread fit and small gaps reduce culture medium residue and impurity accumulation, facilitating cleaning and sterilization, and making it more suitable for… The aseptic requirements for stem cell gel preparation, while considering ease of operation and equipment reliability, dictate that the top of the centrifuge liner 51 is higher than the top of the centrifuge chamber 4. The mounting assembly 53 consists of a fixed sleeve 531, a movable sleeve 532, and symmetrically arranged limiting components 533. The fixed sleeve 531 is embedded within the surface of the centrifuge liner 51, and the movable sleeve 532 is embedded within the rotor hole 54. The symmetrically arranged limiting components 533 are located at the connection between the movable sleeve 532 and the rotor hole 54. When the centrifuge liner 51 needs to be disassembled, rotating the exposed portion of the movable sleeve 532 causes the centrifuge liner 51 to move upwards via the threaded connection at the contact point between the fixed sleeve 531 and the movable sleeve 532. The core bushing 51 is separated from the rotor hole 54. The fixed sleeve 531 is a hollow cylindrical structure. The outer surface of the fixed sleeve 531 is threaded. The inner wall of the movable sleeve 532 is threaded. The inner wall of the movable sleeve 532 is threaded to the outer surface of the fixed sleeve 531. The limiting component 533 consists of a limiting rotating block 5331 and a limiting rotating groove 5332. The limiting rotating groove 5332 is opened on the inner wall of the rotor hole 54. The limiting rotating block 5331 is sleeved and fixed on the surface of the movable sleeve 532. The limiting rotating block 5331 and the limiting rotating groove 5332 are in a snap-fit ​​state. The limiting component 533 is used to limit the rotation of the movable sleeve 532 when it rotates inside the rotor hole 54. The top of the main unit housing 1 is provided with a top cover 2.

[0016] The working principle and usage process of this utility model are as follows: During operation, the protective sleeve 3, centrifuge chamber 4, centrifuge rotor 5, centrifuge tubes, and other structures of the device are disinfected in the laminar flow hood. The operator wears sterile clothing and gloves to ensure a sterile environment throughout the process. According to the diameter of the centrifuge tube, the centrifuge bushing 51 with an inner diameter that matches the diameter of the centrifuge tube is installed by rotating the installation component 53. The stem cell suspension to be processed is transferred into the sterile centrifuge tube. The weight of the centrifuge tube with symmetrical holes is balanced according to the equipment requirements. The tube is then placed steadily into the centrifuge bushing 51 and confirmed to be locked. The centrifugation parameters are set through the control panel. The top cover 2 of the device is closed and the device is started. The drive motor drives the rotor to rotate at high speed. Centrifugal force is used to separate the stem cells from the culture medium, dead cells, and other impurities into layers. The device monitors the rotation speed, temperature, and operating status in real time. After centrifugation, the top cover 2 is opened after the rotor has completely stopped. The centrifuge tube is taken out in the laminar flow hood, the upper supernatant is discarded, and the concentrated stem cell precipitate at the bottom of the tube is collected. The stem cell precipitate is resuspended in culture medium for subsequent mixing with gel matrix such as gelatin to prepare gel. The device is then cleaned for subsequent use.

[0017] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A centrifugal processing device for preparing stem cell gels, comprising a centrifugal processing mechanism disposed inside the main unit housing (1), characterized in that: The centrifugal processing mechanism consists of a sheath (3), a centrifugal chamber (4), and a centrifugal rotor (5). The centrifugal chamber (4) is located at the top of the main unit housing (1). The sheath (3) is located at the top of the main unit housing (1). The centrifugal rotor (5) is located inside the centrifugal chamber (4). Multiple rotor hole assemblies are provided on the inner side of the centrifugal rotor (5). The multiple rotor hole assemblies consist of a centrifugal bushing (51), a silicone seat (52), an installation component (53), and a rotor hole (54). The rotor hole (54) is located at the top of the centrifugal chamber (4). The centrifugal bushing (51) is located inside the rotor hole (54). The installation component (53) is located at the connection between the centrifugal bushing (51) and the rotor hole (54). The silicone seat (52) is located at the connection between the bottom end of the centrifugal bushing (51) and the bottom end of the rotor hole (54).

2. The centrifugation apparatus for preparing stem cell gels according to claim 1, characterized in that: The top of the centrifugal bushing (51) is higher than the top of the centrifugal chamber (4).

3. The centrifugation apparatus for preparing stem cell gels according to claim 1, characterized in that: The mounting assembly (53) consists of a fixed sleeve (531), a movable sleeve (532), and symmetrically arranged limiting components (533). The fixed sleeve (531) is embedded in the surface of the centrifugal bushing (51), the movable sleeve (532) is embedded in the inner side of the rotor hole (54), and the symmetrically arranged limiting components (533) are located at the connection between the movable sleeve (532) and the inner side of the rotor hole (54).

4. The centrifugal processing device for preparing stem cell gel according to claim 3, characterized in that: The fixing sleeve (531) is a hollow cylindrical structure, and the outer surface of the fixing sleeve (531) is threaded.

5. The centrifugation apparatus for preparing stem cell gels according to claim 3, characterized in that: The inner wall of the movable sleeve (532) is threaded, and the inner wall of the movable sleeve (532) is threadedly connected to the outer surface of the fixed sleeve (531).

6. The centrifugation apparatus for preparing stem cell gels according to claim 3, characterized in that: The limiting component (533) consists of a limiting rotating block (5331) and a limiting rotating groove (5332). The limiting rotating groove (5332) is opened on the inner wall of the rotor hole (54). The limiting rotating block (5331) is sleeved and fixed on the surface of the movable sleeve (532). The limiting rotating block (5331) and the limiting rotating groove (5332) are in a locking state.

7. The centrifugation apparatus for preparing stem cell gels according to claim 1, characterized in that: The top of the main unit housing (1) is provided with a top cover (2).

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