A vacuum-assisted transient genetic transformation device for plant tissues

Abstract

This invention discloses a vacuum-assisted transient genetic transformation device for plant tissues, comprising a container body, a lid, and a filter assembly. The container body has a receiving cavity and an opening communicating with the receiving cavity. The lid is detachably fitted onto the opening and has a suction hole communicating with the receiving cavity. The filter assembly includes a first filter plate and a second filter plate, both disposed within the receiving cavity. The first filter plate is spaced apart from the bottom wall of the receiving cavity and has multiple first through holes. The periphery of the first filter plate is sealed to the periphery of the receiving cavity. The second filter plate is spaced above the first filter plate and has multiple second through holes, which are offset from the first through holes. This invention prevents the solution in the container from draining through the suction hole, improving the water-blocking effect and increasing the transient genetic transformation efficiency of plant tissues.

Description

Technical Field

[0001] This utility model relates to the field of experimental equipment, and in particular to a vacuum-assisted transient genetic transformation device for plant tissues. Background Technology

[0002] In some biological experiments (such as transient transformation experiments of plant tissues), it is necessary to use vacuum equipment to evacuate the experimental container. However, when there is a solution in the experimental container, the solution in the experimental container is easily drawn towards the suction port and into the vacuum equipment during the vacuuming process, which affects the experimental equipment and the efficiency of the experimental transformation. Utility Model Content

[0003] The main objective of this invention is to provide a vacuum-assisted transient genetic transformation device for plant tissues, designed to prevent the solution in the container from being discharged through the suction hole.

[0004] To achieve the above objectives, this utility model proposes a vacuum-assisted transient genetic transformation device for plant tissues, comprising:

[0005] The container body has a receiving cavity and an opening communicating with the receiving cavity;

[0006] A cover, detachably fitting onto the opening, the cover having a suction hole communicating with the receiving cavity; and

[0007] The filter assembly includes a first filter plate and a second filter plate, both of which are disposed within the receiving cavity. The first filter plate is spaced apart from the bottom wall of the receiving cavity and has a plurality of first through holes. The periphery of the first filter plate is sealed to the periphery of the receiving cavity. The second filter plate is spaced above the first filter plate and has a plurality of second through holes. The second through holes are offset from the first through holes.

[0008] Optionally, the first filter plate is provided to gradually protrude towards the bottom wall of the receiving cavity from the periphery towards the center.

[0009] Optionally, the second filter plate is provided to gradually protrude towards the bottom wall of the receiving cavity from the periphery towards the center.

[0010] Optionally, one of the second through holes is located at the lowest point of the second filter plate.

[0011] Optionally, the second filter plate is connected to the cover, and the first filter plate is connected to the second filter plate.

[0012] Optionally, the second filter plate is provided with a plurality of connecting posts, which are distributed at intervals along the circumference of the second filter plate, and each of the plurality of connecting posts is connected to the cover.

[0013] Optionally, the connecting post is detachably connected to the cover; and / or, the connecting post is detachably connected to the second filter plate.

[0014] Optionally, the first filter plate is provided with an upwardly extending buckle, which is engaged with the second filter plate.

[0015] Optionally, the buckle is located on the upper surface of the first filter plate, and the buckle is engaged with the second through hole.

[0016] Optionally, the first filter plate abuts against the peripheral wall of the receiving cavity to achieve a seal, or the periphery of the first filter plate is provided with a sealing ring, which abuts against the peripheral wall of the receiving cavity.

[0017] This invention provides a solution by installing a first filter plate and a second filter plate within the container body. The first filter plate is spaced apart from the bottom wall of the receiving cavity and has multiple first through holes. The periphery of the first filter plate is sealed to the periphery of the receiving cavity. The second filter plate is positioned above the first filter plate and has multiple second through holes, which are offset from the first through holes. During the experiment, the experimental material is placed in the space between the first filter plate and the bottom wall of the receiving cavity. When a vacuum is applied, water droplets in the experimental material flow upwards through the first through holes with the air. Due to inertia, the water droplets directly impact the second filter plate, preventing them from flowing directly into the second through holes. This achieves a water-blocking effect, preventing the solution in the container from draining out through the suction hole and affecting the efficiency of the instantaneous conversion. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of an embodiment of a vacuum-assisted transient genetic transformation device for plant tissues according to the present invention;

[0020] Figure 2 for Figure 1 Exploded view of a vacuum-assisted transient genetic transformation device for plant tissues;

[0021] Figure 3 for Figure 1 A cross-sectional view of a vacuum-assisted transient genetic transformation device for plant tissues;

[0022] Figure 4 for Figure 3Enlarged view of point A in the middle.

[0023] Explanation of icon numbers:

[0024] 10. Container body; 11. Receiving cavity; 111. Bottom wall; 112. Peripheral wall; 12. Opening; 20. Cover; 21. Suction hole; 30. Filter assembly; 31. First filter plate; 311. First through hole; 312. Snap fastener; 32. Second filter plate; 321. Second through hole; 33. Connecting column;

[0025] The realization of the purpose, functional features and advantages of this utility model will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0026] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only a part of the embodiments of the present utility model, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the scope of protection of the present utility model.

[0027] It should be noted that if the embodiments of this utility model involve directional indicators (such as up, down, left, right, front, back, etc.), the directional indicators are only used to explain the relative positional relationship and movement of the components in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicators will also change accordingly.

[0028] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text is to include three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution that simultaneously satisfies A and B. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0029] This invention proposes a vacuum-assisted transient genetic transformation device for plant tissues.

[0030] In the embodiments of this utility model, such as Figures 1 to 4As shown, the vacuum-assisted transient genetic transformation device for plant tissues includes a container body 10, a cover 20, and a filter assembly 30. The container body 10 has a receiving cavity 11 and an opening 12 communicating with the receiving cavity 11. The cover 20 is detachably fitted onto the opening 12 and has a suction hole 21 communicating with the receiving cavity 11. The filter assembly 30 includes a first filter plate 31 and a second filter plate 32. Both the first filter plate 31 and the second filter plate 32 are disposed within the receiving cavity 11. The first filter plate 31 is spaced apart from the bottom wall 111 of the receiving cavity 11 and has a plurality of first through holes 311. The periphery of the first filter plate 31 is sealed to the periphery 112 of the receiving cavity 11. The second filter plate 32 is spaced above the first filter plate 32 and has a plurality of second through holes 321. The second through holes 321 are offset from the first through holes 311.

[0031] The container body 10 can be cylindrical or prismatic, etc., and the shapes of the lid 20 and the first filter plate 31 correspond to the shapes of the container body 10. The lid 20 can be cylindrical (similar to a stopper) or similar to a water bottle cap, as long as it can seal the opening 12. This utility model does not limit this.

[0032] In use, the space between the first filter plate 31 and the bottom wall 111 of the receiving cavity 11 is used to hold the experimental material. The diameters of the first through hole 311 and the second through hole 321 can be the same or different; for example, the diameter of the first through hole 311 may be larger or smaller than the diameter of the second through hole 321. The second through hole 321 is offset from the first through hole 311, meaning that the orthographic projections of the second through hole 321 and the first through hole 311 on the horizontal plane are spaced apart. Thus, during vacuuming, when water droplets flow upwards from the first through hole 311 with the air, the water droplets, under the action of inertia, directly impact the second filter plate 32, while the air can be diverted to flow into the second through hole 321, thereby achieving a water-blocking effect.

[0033] During vacuuming, the suction port 21 can be connected to the suction port of the vacuuming equipment to evacuate the container body 10. Alternatively, the container body 10 can be placed entirely into the vacuum chamber, with the container body 10 connected to the vacuum chamber through the suction port 21 of the cover 20. Vacuuming the vacuum chamber will then evacuate the container body 10, thus avoiding negative pressure on the container body 10 and reducing the structural strength requirements for the container body 10.

[0034] Optionally, the first filter plate 31 abuts against the peripheral wall 112 of the receiving cavity 11 to achieve a seal. That is, the opening 12 of the container body 10 is open, and the first filter plate 31 is inserted into the receiving cavity 11 through the opening 12. In this way, the seal is achieved by the abutment between the first filter plate 31 and the peripheral wall 112 of the receiving cavity 11, resulting in a simple structure. It is understood that due to errors, there may be a small gap between the first filter plate 31 and the peripheral wall 112 of the receiving cavity 11. However, the total area of ​​the multiple first through holes 311 on the first filter plate 31 is much larger than the gap between the first filter plate 31 and the peripheral wall 112 of the receiving cavity 11. The solution flowing out from the gap between the first filter plate 31 and the peripheral wall 112 of the receiving cavity 11 during vacuuming can be ignored.

[0035] In another embodiment, a sealing ring is provided around the periphery of the first filter plate 31, and the sealing ring abuts against the peripheral wall 112 of the receiving cavity 11. This improves the sealing effect between the periphery of the first filter plate 31 and the peripheral wall 112 of the receiving cavity 11.

[0036] This utility model's technical solution involves setting a first filter plate 31 and a second filter plate 32 within the container body 10. The first filter plate 31 is spaced apart from the bottom wall 111 of the receiving cavity 11 and has multiple first through holes 311. The periphery of the first filter plate 31 is sealed to the periphery 112 of the receiving cavity 11. The second filter plate 32 is spaced above the first filter plate 31 and has multiple second through holes 321, which are offset from the first through holes 311. During the experiment, the experimental material is placed in the space between the first filter plate 31 and the bottom wall 111 of the receiving cavity 11. When a vacuum is applied, water droplets in the experimental material flow upwards through the first through holes 311 with the air. Under inertia, the water droplets directly impact the second filter plate 32, preventing the water droplets in the air from flowing directly into the second through holes 321, thus achieving a water-blocking effect and preventing water from the container from being discharged through the suction hole 21.

[0037] In some embodiments, the first filter plate 31 is gradually convex toward the bottom wall 111 of the receiving cavity 11 from the periphery toward the center. That is, the surface of the first filter plate 31 is a downwardly convex spherical or conical shape. This allows water droplets adhering to the lower surface of the first filter plate 31 to gradually gather toward the center, facilitating the formation of large water droplets that drip off. This avoids the accumulation of a large number of water droplets on the lower surface of the first filter plate 31 for a long time, further reducing the risk of water droplets flowing upward through the first through hole 311 with the air, and improving the water-blocking effect.

[0038] In some embodiments, the second filter plate 32 is gradually convex toward the bottom wall 111 of the receiving cavity 11 from the periphery toward the center. That is, the surface of the second filter plate 32 is a downwardly convex spherical or conical shape, which allows water droplets adhering to the lower surface of the second filter plate 32 to gradually gather toward the center, facilitating the formation of large water droplets that drip off, avoiding the accumulation of a large number of water droplets on the lower surface of the second filter plate 32 for a long time, further reducing the risk of water droplets flowing upward through the second through hole 321 with the air, and improving the water-blocking effect.

[0039] In some embodiments, one of the second through holes 321 is located at the lowest point of the second filter plate 32. This allows water droplets on the second filter plate 32 to drip onto the first filter plate 31, preventing water droplets from accumulating on the second filter plate 32 and reducing the possibility of water droplets flowing with the airflow to the suction hole 21.

[0040] In some embodiments, the second filter plate 32 is connected to the cover 20, and the first filter plate 31 is connected to the second filter plate 32. That is, during assembly, the first filter plate 31 can be installed on the second filter plate 32 first, the second filter plate 32 on the cover 20, and finally the cover 20 is closed over the opening 12. This avoids the need for a filter plate assembly installation structure within the container body 10, simplifies the structure of the container body 10, and makes the inner wall of the receiving cavity 11 smooth, facilitating cleaning after the experiment. Of course, in other embodiments, both the first filter plate 31 and the second filter plate 32 can be installed on the container body 10.

[0041] In some embodiments, the second filter plate 32 is provided with a plurality of connecting posts 33, which are distributed at intervals along the circumference of the second filter plate 32, and all the connecting posts 33 are connected to the cover 20. This structure is simple and can ensure the stable installation of the second filter plate 32 and the first filter plate 31.

[0042] Optionally, the connecting column 33 and the cover 20 are detachably connected; this allows the filter assembly 30 to be separated from the cover 20 for easy cleaning after the experiment, and also makes it easy to replace different types of filter assemblies 30 (e.g., filter assemblies 30 with different pore sizes), thus providing high scalability.

[0043] Optionally, the connecting column 33 and the second filter plate 32 can be detached, so that the filter assembly 30 and the cover 20 can be separated for easy cleaning after the experiment, and it is also convenient to replace different types of filter assemblies 30 (such as filter assemblies 30 with different pore sizes), which has high scalability.

[0044] In some embodiments, the first filter plate 31 is provided with an upwardly extending buckle 312, which engages with the second filter plate 32. This facilitates the assembly and disassembly of the first filter plate 31 and the second filter plate 32. Of course, in other embodiments, the first filter plate 31 can also be fixed to the second filter plate 32 with screws.

[0045] In some embodiments, the snap fastener 312 is located on the upper surface of the first filter plate 31 and snaps onto the second through hole 321. That is, the second through hole 321 can be directly used to connect with the snap fastener 312, avoiding the need to set a separate snap-fit ​​structure field on the second filter plate 32, which simplifies the structure of the second filter plate 32.

[0046] The above description is only a preferred embodiment of the present utility model and does not limit the patent scope of the present utility model. All equivalent structural transformations made under the inventive concept of the present utility model using the contents of the present utility model specification and drawings, or direct / indirect applications in other related technical fields, are included within the patent protection scope of the present utility model.

Claims

1. A vacuum-assisted transient genetic transformation device for plant tissues, characterized in that, include: The container body has a receiving cavity and an opening communicating with the receiving cavity; A cover, detachably fitted to the opening, the cover having a suction hole communicating with the receiving cavity; and a filter assembly, including a first filter plate and a second filter plate, both the first and second filter plates being disposed within the receiving cavity, the first filter plate being spaced apart from the bottom wall of the receiving cavity and having a plurality of first through holes, the periphery of the first filter plate being sealed to the periphery of the receiving cavity, the second filter plate being spaced apart above the first filter plate and having a plurality of second through holes, the second through holes being offset from the first through holes.

2. The vacuum-assisted transient genetic transformation device for plant tissues as described in claim 1, characterized in that, The first filter plate is arranged to gradually protrude towards the bottom wall of the receiving cavity from the periphery towards the center.

3. The vacuum-assisted transient genetic transformation device for plant tissues as described in claim 2, characterized in that, The second filter plate is arranged to gradually protrude towards the bottom wall of the receiving cavity from the periphery towards the center.

4. The vacuum-assisted transient genetic transformation device for plant tissues as described in claim 3, characterized in that, One of the second through holes is located at the lowest point of the second filter plate.

5. The vacuum-assisted transient genetic transformation device for plant tissues as described in claim 1, characterized in that, The second filter plate is connected to the cover, and the first filter plate is connected to the second filter plate.

6. The vacuum-assisted transient genetic transformation device for plant tissues as described in claim 5, characterized in that, The second filter plate is provided with a plurality of connecting posts, which are distributed at intervals along the circumference of the second filter plate, and each of the plurality of connecting posts is connected to the cover.

7. The vacuum-assisted transient genetic transformation device for plant tissues as described in claim 6, characterized in that, The connecting post is detachably connected to the cover; and / or, the connecting post is detachably connected to the second filter plate.

8. The vacuum-assisted transient genetic transformation device for plant tissues as described in claim 5, characterized in that, The first filter plate is provided with an upwardly extending buckle, which is engaged with the second filter plate.

9. A vacuum-assisted transient genetic transformation device for plant tissues as described in claim 8, characterized in that, The buckle is located on the upper surface of the first filter plate and is engaged with the second through hole.

10. The vacuum-assisted transient genetic transformation device for plant tissues as described in claim 1, characterized in that, The first filter plate abuts against the peripheral wall of the receiving cavity to achieve a seal, or the first filter plate is provided with a sealing ring at its periphery, and the sealing ring abuts against the peripheral wall of the receiving cavity.

Images