A bioreactor for plant tissue culture

Abstract

This utility model relates to the field of plant tissue culture technology and provides a bioreactor for plant tissue culture, including a box and a liquid carrier. The box includes a culture zone and a liquid exchange zone. The culture zone is provided with a cover, and the liquid exchange zone is provided with a liftable support seat. The liquid carrier is provided with a disposable inner liner for containing culture medium, and the culture zone is provided with a tray for placing plant tissue. When changing the culture medium, the support seat is first moved down to the lower end of the lifting stroke, so that the liquid carrier is moved into the liquid exchange zone. Then, the liquid carrier is removed and the disposable inner liner is removed. Next, a new disposable inner liner is replaced and an appropriate amount of the target type of culture medium is injected. Then, the liquid carrier is placed back on the support seat. Finally, the support seat is moved up and reset to the upper end of the lifting stroke. The operation is simple, and there is no need to clean the culture container or repeatedly transfer the cultured plant tissue, which greatly improves the convenience and efficiency of culture medium replacement.

Description

Technical Field

[0001] This utility model relates to the field of plant tissue culture technology, specifically a bioreactor for plant tissue culture. Background Technology

[0002] In plant research, plant tissue culture technology plays a crucial role. This technology enables rapid plant propagation, genetic improvement, and other goals by culturing plant cells, tissues, or organs under sterile conditions.

[0003] Currently, most plant tissue culture processes use petri dishes or flasks as culture containers (i.e., bioreactors). At different stages of plant tissue culture, the plant tissue's requirements for nutrients and growth regulators change significantly, requiring the replacement of different types of culture media. When changing the culture media, the operator needs to first remove the plant tissue from the culture container, then pour out the original culture media and clean the culture container, then inject new culture media into the cleaned culture container, and finally put the plant tissue back into the culture container. This series of operations is very tedious, complicated, time-consuming, and labor-intensive.

[0004] Therefore, this invention proposes a bioreactor for plant tissue culture to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a bioreactor for plant tissue culture to solve the above-mentioned problems.

[0006] To achieve the above objectives, this utility model provides the following technical solution: A bioreactor for plant tissue culture includes a housing and a liquid-carrying component. The housing includes a culture zone and a liquid-changing zone. The culture zone is covered, and the liquid-changing zone is open on one side. A liftable support is provided in the liquid-changing zone for placing the liquid-carrying component. The liquid-carrying component contains a disposable inner liner for containing culture medium. The culture zone contains a tray for placing plant tissue. When the support is at the upper end of its lifting stroke, the tray is located in the disposable inner liner. When the support is at the lower end of its lifting stroke, the liquid-carrying component is located in the liquid-changing zone.

[0007] In one alternative: the support seat is provided with an inner groove adapted to the liquid-carrying component.

[0008] In one alternative: both sides of the fluid exchange area are provided with vertically oriented through grooves, and both sides of the support seat are provided with handles adapted to the through grooves, with the end of the handle away from the support seat passing through the corresponding through groove; A first magnetic ring is provided at the junction of the culture zone and the medium exchange zone, and a second magnetic ring adapted to the first magnetic ring is provided on the support.

[0009] In one alternative: at least one of the first and second magnetic rings is covered with a soft adhesive layer.

[0010] In one alternative: the loading tray is detachably disposed in the culture area, the loading tray is provided with a support rod, the culture area is provided with a mounting base, the mounting base is provided with a slot adapted to the support rod, for the end of the support rod away from the loading tray to be engaged, and the mounting base is also provided with bolts for abutting the support rod to fix it.

[0011] Compared with the prior art, the beneficial effects of this utility model embodiment are as follows: During plant tissue culture, the support is located at the upper end of the lifting stroke, the culture medium is located in the disposable inner liner, and the tray is located in the disposable inner liner to ensure contact between the plant tissue and the culture medium. When the culture medium needs to be changed, first move the support to the lower end of the lifting stroke, so that the liquid carrier is moved to the medium change area. Then, remove the liquid carrier and the disposable inner liner. Next, replace it with a new disposable inner liner and inject an appropriate amount of the target type of culture medium. Then, place the liquid carrier back on the support. Finally, move the support back up to the upper end of the lifting stroke. The operation is simple, and there is no need to clean the culture container or repeatedly transfer the cultured plant tissue, which greatly improves the convenience and efficiency of culture medium replacement.

[0012] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application. Attached Figure Description

[0013] The accompanying drawings, which are incorporated in and form part of this specification, illustrate embodiments consistent with this application and, together with the description, serve to explain the principles of this application. Furthermore, these drawings and textual descriptions are not intended to limit the scope of the concept in any way, but rather to illustrate the concepts of this application to those skilled in the art through reference to specific embodiments.

[0014] Figure 1 This is a structural schematic diagram of an embodiment of the present utility model.

[0015] Figure 2 This is a schematic diagram of the housing configuration in an embodiment of this utility model.

[0016] Figure 3 This is a schematic diagram showing the arrangement of the support base, liquid-carrying component, and disposable inner liner in an embodiment of this utility model.

[0017] Figure 4 This is a schematic diagram showing the arrangement of the tray, support rod, and mounting base in an embodiment of this utility model.

[0018] Figure label annotations: 1-box body, 101-culture area, 102-liquid replacement area, 2-liquid cover, 3-support seat, 4-liquid carrier, 5-through groove, 6-handle rod, 7-mounting seat, 8-support rod, 9-bolt, 10-dispensing tray, 11-slot, 12-first magnetic ring, 13-disposable inner liner, 14-inner groove, 15-second magnetic ring. Detailed Implementation

[0019] The present application will now be described in further detail with reference to the accompanying drawings. It should be noted that the following specific embodiments are only used to further illustrate the present application and should not be construed as limiting the scope of protection of the present application. Those skilled in the art can make some non-essential improvements and adjustments to the present application based on the above application content.

[0020] Please see Figures 1-3 A bioreactor for plant tissue culture includes a housing 1 and a liquid-carrying component 4. The housing 1 includes a culture zone 101 and a liquid-changing zone 102. The culture zone 101 is provided with a cover 2. The liquid-changing zone 102 is open on one side and is provided with a liftable support 3 for placing the liquid-carrying component 4. The liquid-carrying component 4 is provided with a disposable inner liner 13 for containing culture medium. The culture zone 101 is provided with a tray 10 for placing plant tissue. When the support 3 is at the upper end of the lifting stroke, the tray 10 is located in the disposable inner liner 13. When the support 3 is at the lower end of the lifting stroke, the liquid-carrying component 4 is located in the liquid-changing zone 102.

[0021] It should be noted that the tray 10 is made of a mesh plate, and the disposable inner liner 13 is a consumable material, which can be made of polystyrene plastic, polycarbonate plastic, or other materials that do not interfere with plant tissue culture. This application does not limit it here. In addition, the disposable inner liner 13 is compatible with the liquid carrier 4, and its specifications and dimensions can be customized. The corresponding manufacturing methods are existing mature technologies and there are no technical obstacles.

[0022] During plant tissue culture, the support seat 3 is located at the upper end of the lifting stroke, the culture medium is located in the disposable inner liner 13, and the tray 10 is located in the disposable inner liner 13 to achieve contact between the plant tissue and the culture medium. When the culture medium needs to be replaced, the support seat 3 is first moved down to the lower end of the lifting stroke, so that the liquid carrier 4 is moved to the medium replacement area 102. Then, the liquid carrier 4 is removed (moved to the outside of the medium replacement area 102) and the disposable inner liner 13 is removed. Next, a new disposable inner liner 13 is installed and an appropriate amount of the target type of culture medium is injected. Then, the liquid carrier 4 is placed back on the support seat 3. Finally, the support seat 3 is moved up and reset to the upper end of the lifting stroke. The operation is simple and does not require cleaning the culture container or repeatedly transferring the cultured plant tissue, which greatly improves the convenience and efficiency of culture medium replacement.

[0023] Furthermore, the cover 2 is provided with a number of ventilation holes (not shown in the figure), and each ventilation hole is filled with filter cotton.

[0024] Furthermore, the support seat 3 is provided with an inner groove 14 adapted to the liquid carrier 4. When the liquid carrier 4 is placed, its bottom is embedded in the inner groove 14, thereby limiting the liquid carrier 4 in the horizontal direction.

[0025] Please see Figures 1-3 In one embodiment of the present invention, the fluid exchange area 102 is provided with vertically oriented through grooves 5 on both sides, and the support seat 3 is provided with grips 6 on both sides that are adapted to the through grooves 5. The end of the grips 6 away from the support seat 3 passes through the corresponding through grooves 5. A first magnetic ring 12 is provided at the junction of the culture zone 101 and the medium exchange zone 102, and a second magnetic ring 15 adapted to the first magnetic ring 12 is provided on the support 3.

[0026] In this embodiment, when the support seat 3 is raised to the upper end of the lifting stroke, the support seat 3 is fixed by the magnetic attraction between the first magnetic ring 12 and the second magnetic ring 15. When the support seat 3 needs to be moved down, the operator holds the two handles 6 with both hands and presses down to overcome the magnetic attraction between the first magnetic ring 12 and the second magnetic ring 15, thereby moving the support seat 3 down.

[0027] Furthermore, in this embodiment, at least one of the first magnetic ring 12 and the second magnetic ring 15 is covered with a soft adhesive layer. When the first magnetic ring 12 and the second magnetic ring 15 are attracted to each other, the soft adhesive layer plays a sealing role, preventing external dust or other foreign objects from entering the culture area 101.

[0028] Please see Figure 1 and Figure 4 In one embodiment of the present invention, the loading tray 10 is detachably disposed in the culture area 101. The loading tray 10 is provided with a support rod 8. The culture area 101 is provided with a mounting seat 7. The mounting seat 7 is provided with a slot 11 adapted to the support rod 8 for the end of the support rod 8 away from the loading tray 10 to be inserted. The mounting seat 7 is also provided with a bolt 9 for pressing the support rod 8 to fix it.

[0029] In this embodiment, the tray 10 can be removed from the culture area 101 to facilitate cleaning of the tray 10. The tray 10 is installed by inserting the end of the support rod 8 away from the tray 10 into the slot 11 on the mounting base 7 and tightening the bolt 9. When disassembling, the bolt 9 can be loosened. In addition, it should be noted that the radial cross section of the support rod 8 is square.

[0030] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A bioreactor for plant tissue culture, characterized in that, The container includes a box (1) and a liquid carrier (4). The box (1) includes a culture area (101) and a liquid exchange area (102). The culture area (101) is provided with a cover (2). The liquid exchange area (102) is open on one side and is provided with a liftable support (3) for placing the liquid carrier (4). The liquid carrier (4) is provided with a disposable inner liner (13) for containing culture medium. The culture area (101) is provided with a tray (10) for placing plant tissue. When the support (3) is at the upper end of the lifting stroke, the tray (10) is located in the disposable inner liner (13). When the support (3) is at the lower end of the lifting stroke, the liquid carrier (4) is located in the liquid exchange area (102).

2. The bioreactor for plant tissue culture according to claim 1, characterized in that, The bearing seat (3) is provided with an inner groove (14) adapted to the liquid carrier (4).

3. The bioreactor for plant tissue culture according to claim 1, characterized in that, The fluid exchange area (102) is provided with vertically oriented through grooves (5) on both sides, and the support seat (3) is provided with handles (6) adapted to the through grooves (5) on both sides. The end of the handle (6) away from the support seat (3) passes through the corresponding through groove (5). A first magnetic ring (12) is provided at the junction of the culture area (101) and the medium exchange area (102), and a second magnetic ring (15) adapted to the first magnetic ring (12) is provided on the support seat (3).

4. The bioreactor for plant tissue culture according to claim 3, characterized in that, At least one of the first magnetic ring (12) and the second magnetic ring (15) is covered with a soft adhesive layer.

5. The bioreactor for plant tissue culture according to claim 1, characterized in that, The loading tray (10) is detachably installed in the culture area (101). The loading tray (10) is provided with a support rod (8). The culture area (101) is provided with a mounting seat (7). The mounting seat (7) is provided with a slot (11) that is adapted to the support rod (8) for the end of the support rod (8) away from the loading tray (10) to be inserted. The mounting seat (7) is also provided with a bolt (9) for pressing the support rod (8) to fix it.

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