A large-volume cell culture bag

Abstract

This utility model belongs to the technical field of cell culture equipment, specifically disclosing a large-volume cell culture bag, which includes a bag body, a filling column, and a clamp. The filling column has a first connecting part and a second connecting part. The inner wall of the first connecting part has internal threads, which are used for connecting or separating from a culture medium bottle. The second connecting part is connected to the bag body via a connecting hose, and the interior of the filling column communicates with the interior of the bag body through the connecting hose. The clamp is disposed on the connecting hose and is used to disconnect the communication between the filling column and the bag body. This utility model's large-volume cell culture bag, through the filling column, allows the culture medium bottle to be directly connected to the filling column during replenishment operations, enabling direct injection of the culture medium from the bottle into the bag body. Each replenishment can be completed in a single operation, eliminating the need for multiple injections, greatly reducing the risk of contamination, and providing convenient replenishment with a higher level of aseptic assurance.

Description

Technical Field

[0001] This utility model relates to the field of cell culture equipment technology, specifically to a large-volume cell culture bag. Background Technology

[0002] Large-volume cell culture bags are mainly used for large-scale cell culture, with a typical capacity between 100 mL and 10 L.

[0003] Traditional cell culture bags typically consist of a bag body, tubing connected to the bag body, and a sealing cap for sealing the tubing. When refilling is needed, the culture medium is usually injected into the bag through the tubing using a syringe. However, for refilling large-volume cell culture bags, each refill requires multiple operations of drawing culture medium from the culture bottle and then injecting it into the cell culture bag, which greatly increases the risk of bacterial contamination. Furthermore, the syringe needs to be changed for each refill operation, making the refill operation inconvenient, and the tubing is also relatively thin, resulting in a long refill time. Utility Model Content

[0004] In view of this, the present invention provides a large-volume cell culture bag with a filling column, thereby solving or at least alleviating one or more of the above-mentioned problems and other problems existing in the prior art.

[0005] To achieve the aforementioned objectives, the technical solution adopted by this utility model is as follows: A large-volume cell culture bag, the cell culture bag comprising: Bag body; A filling column having a first connecting portion and a second connecting portion, wherein the inner wall of the first connecting portion has an internal thread for engaging or disengaging with a culture medium bottle; the second connecting portion is connected to the bag body via a connecting hose, and the interior of the filling column communicates with the interior of the bag body via the connecting hose. A clip, which is disposed on the connecting hose and is also removable from the connecting hose, is used to disconnect the connection between the filling column and the bag body.

[0006] In the aforementioned large-volume cell culture bag, optionally, the filling column includes a column body, a first connecting portion formed at one end of the column body, a conical portion formed at the other end of the column body, and a second connecting portion formed on the conical portion, wherein the cross-sectional area of ​​the conical portion gradually decreases from the end connected to the column body to the end connected to the second connecting portion.

[0007] Optionally, the column body is a cylinder.

[0008] Optionally, the inner diameter of the column body is 2.5 ± 0.5 cm.

[0009] Optionally, the cell culture bag further includes a support column holder disposed on the column body, the support column holder being used to fix the filling column relative to other equipment.

[0010] Optionally, the filling column is a transparent filling column made of plastic.

[0011] Optionally, the two ends of the connecting hose are welded to the second connecting part and the bag body, respectively.

[0012] Optionally, in the aforementioned large-volume cell culture bag, the outer surface of the first connecting portion has external threads, and the cell culture bag further includes a sealing cap connected to the external threads.

[0013] Optionally, in the large-volume cell culture bag, the volume of the bag is 1L or more.

[0014] Optionally, the volume of the bag is 1.5L to 5L.

[0015] Due to the application of the above technical solution, this utility model has the following advantages compared with the prior art: This utility model's large-volume cell culture bag, through the setting of the filling column, allows the culture medium bottle to be directly connected to the filling column during the replenishment operation, so that the culture medium in the culture medium bottle can be directly injected into the bag. Each replenishment can be completed in one operation, eliminating the need for multiple injections, greatly reducing the risk of bacterial contamination, and making replenishment convenient while ensuring a higher level of aseptic protection. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the structure of a large-volume cell culture bag according to an embodiment of the present invention; Figure 2 for Figure 1 A schematic diagram of the filling column for a large-volume cell culture bag; Figure 3 for Figure 1 A schematic diagram of the structure of a large-volume cell culture bag during fluid replenishment; In the diagram: 1. Bag body; 2. Filling column; 2a. Column body; 2b. First connecting part; 2c. Conical part; 2d. Second connecting part; 2e. External thread; 3. Connecting hose; 4. Clip; 5. Support column holder; 6. Culture medium bottle; 7. Sealing cap. Detailed Implementation

[0018] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0019] When large-volume cell culture bags are used for cell culture, especially those larger than 1.5L, a certain amount of culture medium needs to be replenished daily. However, traditional large-volume cell culture bags require the culture medium to be drawn from the culture medium bottle with a syringe and then injected into the cell culture bag. This process may pose a risk of bacterial contamination. For larger volumes of culture medium each time, multiple injections are required, which greatly increases the risk of bacterial contamination. In addition, a new syringe is needed for each injection, making the replenishment process inconvenient and time-consuming.

[0020] Based on this, this application redesigns the structure of the large-volume cell culture bag. During replenishment, the corresponding culture medium bottle can be selected according to the amount of culture medium to be replenished, and the culture medium bottle can be directly connected to the cell culture bag to inject the culture medium in the culture medium bottle directly into the cell culture bag. This avoids the intermediate operation of aspirating and injecting with a syringe, greatly reducing the risk of bacterial contamination. Moreover, the initial culture medium in the cell culture bag can also be directly connected to the cell culture bag through the culture medium bottle to inject the initial culture medium in the culture bag directly into the cell culture bag, further reducing the risk of bacterial contamination.

[0021] The present invention will now be described in further detail with reference to the accompanying drawings.

[0022] See Figure 1 The large-volume cell culture bag shown includes a bag body 1, which is used for cell culture.

[0023] As shown in the figure, the large-volume cell culture bag also includes a filling column 2 and a sealing cap 7. The interior of the filling column 2 is connected to the interior of the bag body 1 through a connecting hose 3. The culture medium bottle can be directly connected to the filling column 2 so that the culture medium in the culture medium bottle can be added to the bag body 1 through the filling column and connecting hose. This avoids the operation of drawing the culture medium from the culture medium bottle with a syringe and then injecting it into the bag body 1, which greatly reduces the risk of contamination.

[0024] The filling column 2 is a transparent filling column made of plastic, such as the same material as a syringe. The connecting tubing can also be welded to the filling column 2 and the bag body 1, such as by heat welding.

[0025] Figure 2 The specific structure of the filling column 2 is shown in the figure. As can be seen from the figure, the filling column 2 includes a column body 2a, a first connecting part 2b formed at one end of the column body 2a, a conical part 2c formed at the other end of the column body 2a, and a second connecting part 2d formed at the end of the conical part 2c. The cross-sectional area of ​​the conical part 2c gradually decreases from the end connected to the column body 2a to the end connected to the second connecting part 2d, which facilitates material feeding. The connecting hose 3 is connected to the second connecting part 2d.

[0026] The first connecting part 2b, the column body 2a, the cone part 2c, and the second connecting part 2d are integrally formed.

[0027] The inner wall of the first connecting part 2b has an internal thread for connecting or separating from the culture medium bottle. The outer surface of the first connecting part 2b has an external thread 2e for connecting or separating from the sealing cap 7. The sealing cap 7 is used to seal the filling column 2 to prevent contamination inside the filling column 2.

[0028] In some alternative embodiments, the column body 2a is a cylinder, and the inner diameter of the column body 2a is 2.5±0.5cm.

[0029] Then by Figure 1 As can be seen, the large-volume cell culture bag also includes a support column holder 5 disposed on the column body 2a. The support column holder 5 is used to fix the filling column 2 relative to other equipment, so as to fix the cell culture bag relatively.

[0030] The large-volume cell culture bag also includes a clip 4, which is attached to the connecting tubing 3 and can be removed from the connecting tubing 3. The clip 4 is used to disconnect the communication between the interior of the filling column 2 and the interior of the bag body 1. The clip 4 can be a plastic clip or a metal clip and can be reused.

[0031] The volume of the bag body 1 of the large-volume cell culture bag is more than 1L, and more preferably, the volume of the bag body 1 is 1.5L to 5L.

[0032] For example, for a large-volume cell culture bag with a volume of 2L, an initial culture medium of 500mL, and a replenishment of more than 50mL each time, the specific operation is as follows: Before using this large-volume cell culture bag, keep the work surface clean and free of sharp objects to prevent damage to the bag. Then, place the support on the work surface to ensure it is stable.

[0033] Remove the sealing cap 7, then align the mouth of the culture medium bottle containing 500 mL of culture medium with the first connection part of the filling column 2 and tighten it.

[0034] The support column 5 of the large-volume cell culture bag is fixed to the bracket, ensuring that the filling column and the bag are upright (e.g., Figure 3As shown in the figure, while ensuring the filling column is stable, remove clamp 4 to facilitate the introduction of culture medium from the culture medium bottle into the bag through the filling column, avoiding the operation of drawing and injecting culture medium with a syringe, which greatly reduces the risk of contamination.

[0035] After the initial culture medium has been poured out, remove the culture medium bottle, fill the column, put on the sealing cap, clamp 4 onto the connecting tubing, and begin cell culture.

[0036] When replenishment of culture medium is required during cell culture, remove the filling column, then remove the sealing cap, connect the culture medium bottle to the filling column, re-secure the filling column, remove the clamp, and allow the culture medium in the culture medium bottle to flow into the bag. After replenishment is complete, replace the sealing cap and clamp the bag again.

[0037] Throughout the cell culture process, it may be necessary to replenish the fluid daily. Each time you replenish the fluid, simply repeat the steps described above until the cell culture is complete.

[0038] When cell culture is complete and the culture medium needs to be removed from the bag, it can be done by pouring the culture medium out through the filling column, or by cutting the connecting tubing and pouring the culture medium out through the connecting tubing. This method of liquid removal is convenient.

[0039] As can be seen from the above operation steps, both the initial culture medium transfer and the subsequent replenishment operation avoid the use of syringes, which greatly reduces the risk of bacterial contamination. The transfer and replenishment operations are also more convenient and faster, greatly reducing the intensity of manual labor and labor costs.

[0040] This large-volume cell culture bag is particularly suitable for cell culture in cell culture bags that require large single-volume replenishment and frequent replenishment.

[0041] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A large-volume cell culture bag, characterized in that, The cell culture bag includes: Bag body; A filling column having a first connecting portion and a second connecting portion, wherein the inner wall of the first connecting portion has an internal thread for engaging or disengaging with a culture medium bottle; the second connecting portion is connected to the bag body via a connecting hose, and the interior of the filling column communicates with the interior of the bag body via the connecting hose. A clip, which is disposed on the connecting hose and is also removable from the connecting hose, is used to disconnect the connection between the filling column and the bag body.

2. The large-volume cell culture bag according to claim 1, characterized in that, The filling column includes a column body, a first connecting portion formed at one end of the column body, a conical portion formed at the other end of the column body, and a second connecting portion formed on the conical portion. The cross-sectional area of ​​the conical portion gradually decreases from the end connected to the column body to the end connected to the second connecting portion.

3. The large-volume cell culture bag according to claim 2, characterized in that, The column body is a cylinder.

4. The large-volume cell culture bag according to claim 3, characterized in that, The inner diameter of the column body is 2.5 ± 0.5 cm.

5. The large-volume cell culture bag according to claim 2, characterized in that, The cell culture bag also includes a support column holder disposed on the column body, which is used to fix the filling column relative to other equipment.

6. The large-volume cell culture bag according to claim 1, characterized in that, The filling column is a transparent filling column made of plastic.

7. The large-volume cell culture bag according to claim 6, characterized in that, The two ends of the connecting hose are welded to the second connecting part and the bag body, respectively.

8. The large-volume cell culture bag according to claim 1, characterized in that, The outer surface of the first connecting part has external threads, and the cell culture bag also includes a sealing cap connected to the external threads.

9. The large-volume cell culture bag according to claim 1, characterized in that, The volume of the bag is 1L or more.

10. The large-volume cell culture bag according to claim 9, characterized in that, The volume of the bag is 1.5L to 5L.

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