An improved electrochemiluminescence detection cell structure

Abstract

This application discloses an improved electrochemiluminescence detection cell structure, relating to the field of electrochemiluminescence detection cells. It includes an electrochemiluminescence detection cell body, with a sealing cap vertically and movably connected to the top of the body. A temperature monitoring probe is installed at the bottom of the sealing cap. This application allows the sealing cap to be vertically opened or closed according to detection needs. The opened sealing cap can self-limit and heat the liquid inside the electrochemiluminescence detection cell body, thereby matching temperature parameters for different detection or experimental requirements. The temperature is monitored and dynamically adjusted simultaneously by the temperature monitoring probe. Meanwhile, the liquid inside the electrochemiluminescence detection cell body circulates at a uniform flow rate, ensuring that the contact time between the sample and the electrode surface and the mixing ratio of the reaction reagents remain constant, avoiding signal deviations caused by flow rate fluctuations, and thus improving the repeatability of the detection results.

Description

Technical Field

[0001] This application relates to the field of electrochemiluminescence detection cells, and in particular to an improved electrochemiluminescence detection cell structure. Background Technology

[0002] Electrochemiluminescence (ECL) is a product of the combination of chemiluminescence and electrochemical methods. After more than 40 years of research, it has become a very important analytical method, applied in various fields such as immunology, food and water sample testing, and biochemical weapon reagent detection. ECL, also known as electrochemiluminescence, refers to the process by which excited-state substances generated by electron transfer reactions on the electrode surface transition back to the ground state, releasing energy in the form of light emission. ECL analysis is a luminescent analytical technique that directly utilizes electrochemical reactions to form excited-state luminescent bodies or generates light radiation through chemical reactions between electrolysis products or between electrolysis products and a component in the system, thereby achieving the determination of analytes. It is a product of the combination of electrochemistry and chemiluminescence.

[0003] In existing technologies, the electrochemiluminescence detection cell structure has limited functionality during use. For example, the internal reagent temperature control cannot match different detection needs, and the internal detection reagent has poor flowability, resulting in inconsistent reaction conditions.

[0004] A search revealed an electrochemiluminescence detection cell disclosed in Chinese patent literature (publication number: CN205719989U), but it still has the following defects:

[0005] Although the aforementioned electrochemiluminescence detection cell is easy to operate, requiring only adjustment of the injection pump flow rate to ensure that all samples are excited on the working electrode surface, thus eliminating the need to manually determine the distance between the tip of the steel needle and the working electrode, it still has limitations such as limited functionality. For example, the internal reagent temperature control cannot match different detection requirements, and the internal detection reagent has poor flowability, leading to inconsistent reaction conditions. Utility Model Content

[0006] To address issues such as limited functionality, inability of internal reagent temperature control to match different detection needs, and poor flowability of internal detection reagents leading to inconsistent reaction conditions, this application provides an improved electrochemiluminescence detection cell structure.

[0007] The improved electrochemiluminescence detection cell structure provided in this application adopts the following technical solution:

[0008] An improved electrochemiluminescence detection cell structure includes an electrochemiluminescence detection cell body. A sealing cover is vertically and movably connected to the top of the electrochemiluminescence detection cell body. A temperature monitoring probe is installed at the bottom of the sealing cover. An optical window is installed inside the front end of the electrochemiluminescence detection cell body. One side of the electrochemiluminescence detection cell body is connected to the output end of a first transfer pump. The input end of the first transfer pump is connected to a transfer tube. An electric heater is installed inside the transfer tube, and a three-way pipe is installed at the other end of the transfer tube. The other two ports of the three-way pipe are connected to the other side of the electrochemiluminescence detection cell body.

[0009] By adopting the above technical solution, the electric heater heats the liquid circulating inside the transmission tube, causing it to flow back into the electrochemiluminescence detection cell body, so that the liquid temperature inside the electrochemiluminescence detection cell body reaches the test or detection standard.

[0010] Preferably, mounting plates are installed on both sides of the top of the sealing cover, and first connecting brackets are symmetrically installed on the bottom of the mounting plates. The interior of each first connecting bracket is hinged to one end of the connecting arm.

[0011] By adopting the above technical solution, the connecting arm supports the closing or opening operation of the sealing cover, and keeps the sealing cover stable after it is opened.

[0012] Preferably, the other end of the connecting arm is hinged to the top of the movable block, and the movable block is movably connected inside the top of the sliding groove.

[0013] By adopting the above technical solution, the movable block can achieve relative or opposite movement inside the sliding groove, thereby guiding the connecting arm.

[0014] Preferably, the sliding grooves are symmetrically installed on both outer walls of the electrochemiluminescence detection cell body, and male magnetic absorbing plates are symmetrically installed inside the sliding grooves, while female magnetic absorbing plates are installed at the corresponding ends of the movable blocks.

[0015] By adopting the above technical solution, the male and female magnetic absorbing sheets attract each other, thereby limiting the movement of the movable block after it has been moved.

[0016] Preferably, a second transfer pump is installed on the outer wall of the electrochemiluminescence detection cell body near the three-way pipe, and a second transfer pump is also installed on the outer wall of the rear end of the electrochemiluminescence detection cell body.

[0017] By adopting the above technical solution, the second transfer pump serves as the transfer source, thereby discharging the liquid inside the electrochemiluminescence detection cell and injecting the liquid into the electrochemiluminescence detection cell.

[0018] Preferably, the input end of the second transfer pump installed on the side wall of the electrochemiluminescence detection cell body is connected to the electrochemiluminescence detection cell body, and the output end of the second transfer pump installed on the side wall of the electrochemiluminescence detection cell body is connected to a drain pipe.

[0019] By adopting the above technical solution, the second transfer pump completes the conveying operation, thereby completing the replenishment and drainage operations.

[0020] Preferably, the output end of the second transfer pump installed on the outer wall of the rear end of the electrochemiluminescence detection cell body is connected to the electrochemiluminescence detection cell body, and the input end of the second transfer pump installed on the outer wall of the rear end of the electrochemiluminescence detection cell body is connected to one end of the replenishment tube. An outer sleeve is installed on the outside of the replenishment tube, and flexible steel wires are installed at equal intervals between the inner side wall of the outer sleeve and the outer side wall of the replenishment tube.

[0021] By adopting the above technical solution, the combination of flexible steel wire and outer sleeve enhances the strength of the liquid replenishment tube while maintaining its flexibility.

[0022] Preferably, an abutment block is installed at one end of the outer sleeve and one end of the drain pipe. A connector is installed through the inside of the abutment block. One end of the connector near the side wall of the electrochemiluminescence detection cell body is connected to the drain pipe. A replenishment pipe is movably connected through the inside of the connector near the rear end of the electrochemiluminescence detection cell body. Both the replenishment pipe and the drain pipe are corrugated pipe structures. A balloon is installed on the outer wall of the connector. A silicone sealing gasket is adhered to the outer wall of the abutment block near the connector.

[0023] By adopting the above technical solution, when it is necessary to replenish or drain the electrochemiluminescence detection cell, the corresponding operations can be completed through the drain pipe and the replenishment pipe.

[0024] In summary, this application includes at least one of the following beneficial technical effects:

[0025] 1. During use, the sealing cover can be opened or closed vertically according to the detection requirements. After opening, the sealing cover can be self-limiting and can heat the liquid inside the electrochemiluminescence detection cell structure to match the temperature parameters required for different detection or experiments. The temperature is monitored and dynamically adjusted by a temperature monitoring probe. At the same time, the liquid inside the electrochemiluminescence detection cell structure circulates and flows at a constant speed, which ensures that the contact time between the sample and the electrode surface and the mixing ratio of the reaction reagents remain constant, avoiding signal deviation caused by flow rate fluctuations, thereby improving the repeatability of the detection results.

[0026] 2. During use, this utility model can be connected and fixed to the drain port or the inlet of the storage vessel through the drain pipe, the replenishment pipe, the connector and the balloon, and is easy to disassemble. It has good stability during both draining and replenishing processes. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the main structure of this utility model;

[0028] Figure 2 This is a schematic diagram of the rear view structure of this utility model;

[0029] Figure 3 This is a schematic diagram of the disassembled parts of the sealing cap and temperature monitoring probe in this utility model;

[0030] Figure 4 This is a cross-sectional structural diagram of the sliding groove in this utility model;

[0031] Figure 5 This is a schematic diagram of the combined components of the transmission pipe, electric heater, and three-way pipe in this utility model;

[0032] Figure 6 This is a schematic diagram of the combined components of the fluid replenishment tube, outer tube, contact block, connector, balloon, and silicone sealing gasket in this utility model.

[0033] Figure 7 This is a schematic diagram of the cross-sectional structure of the fluid replenishment tube and the outer sleeve in this utility model.

[0034] Reference numerals in the attached drawings: 1. Electrochemiluminescence detection cell body; 2. Sealing cover; 3. First connecting frame; 4. Connecting arm; 5. Movable block; 6. Sliding groove; 7. Male magnetic accumulator; 8. Female magnetic accumulator; 9. Temperature monitoring probe; 10. Optical window;

[0035] 11. First transfer pump; 12. Transfer pipe; 13. Electric heater; 14. T-connector; 15. Second transfer pump; 16. Drain pipe; 17. Replenishment pipe; 18. Outer sleeve; 19. Flexible steel wire; 20. Contact block;

[0036] 21. Connector; 22. Balloon; 23. Silicone sealing gasket. Detailed Implementation

[0037] The following is in conjunction with the appendix Figures 1-7 This application will be described in further detail.

[0038] This application discloses an improved electrochemiluminescence detection cell structure.

[0039] Reference Figures 1-4An improved electrochemiluminescence detection cell structure includes an electrochemiluminescence detection cell body 1. The electrochemiluminescence detection cell body 1 contains at least a working electrode, a reference electrode, and a counter electrode for detection. Sliding grooves 6 are fixedly connected to both outer walls of the electrochemiluminescence detection cell body 1. Convex movable blocks 5 are symmetrically slidably connected inside the sliding grooves 6. A female magnetic absorbing plate 8 is fixedly connected to one end of each movable block 5. A male magnetic absorbing plate 7 is symmetrically fixedly connected inside the sliding grooves 6. Connecting arms 4 are symmetrically hinged to the tops of the movable blocks 5, which are movably connected inside the same sliding grooves 6. The other ends of the connecting arms 4 are hinged to the bottom of a first connecting frame 3. The first connecting frame 3 is symmetrically fixedly connected to the bottom of a sealing cover 2. The sealing cover 2 is vertically movably connected to the top of the electrochemiluminescence detection cell body 1. A temperature monitoring probe 9 is fixedly connected to the bottom of the sealing cover 2.

[0040] During the operation of the electrochemiluminescence detection cell body 1, to avoid the limitations of the electrochemiluminescence detection cell body 1 during use, the temperature parameter data of the liquid inside the electrochemiluminescence detection cell body 1 is obtained in real time through the temperature monitoring probe 9. Then, based on the obtained temperature parameter data, it is analyzed whether it matches the temperature requirements of the current test. According to the sealing requirements of the electrochemiluminescence detection cell body 1 during use, the sealing cover 2 completes the sealing or opening of the electrochemiluminescence detection cell body 1, so that the electrochemiluminescence detection cell body 1 can be operated without limitations. At the same time, when the sealing cover 2 is closed, it completes the sealing operation due to its own weight. When the sealing cover 2 moves vertically, the connecting arms 4, which are symmetrically arranged on both sides, will move. Then, the connecting arms 4 drive the moving block 5 to move inside the sliding groove 6 until the male magnetic absorbing piece 7 and the female magnetic absorbing piece 8 are attached to each other, thereby performing the magnetic attraction and limiting activity of the sealing cover 2.

[0041] Reference Figure 1 , Figure 2 and Figure 5 An optical window 10 is fixedly connected to the front end of the electrochemiluminescence detection cell body 1. A first transfer pump 11 is fixedly connected to the left outer wall of the electrochemiluminescence detection cell body 1, and the output end of the first transfer pump 11 is connected to the electrochemiluminescence detection cell body 1. A transfer tube 12 is fixedly connected to the input end of the first transfer pump 11. An electric heater 13 with an "L" shaped structure is fixedly connected inside the transfer tube 12. The other end of the transfer tube 12 is fixedly connected to one end of a three-way tube 14, and the other two ports of the three-way tube 14 are connected to the electrochemiluminescence detection cell body 1.

[0042] During use, the first transfer pump 11 outputs the work, and then draws the liquid inside the electrochemiluminescence detection cell body 1 from the two ends of the three-way pipe 14 and transfers it into the transfer pipe 12. According to the current detection requirements, the return liquid can be heated by the electric heater 13, and the power of the electric heater 13 can be adjusted in real time by obtaining the temperature parameter data through the temperature monitoring probe 9. Then, the return liquid is transferred to the electrochemiluminescence detection cell body 1 through the first transfer pump 11, thereby effectively driving the flow of liquid inside the electrochemiluminescence detection cell body 1. This can avoid the problem of poor internal detection reagent flow during the use of the device, which leads to inconsistent reaction conditions.

[0043] Reference Figure 1 and Figure 2 A second transfer pump 15 for liquid transport is fixedly connected to the outer wall of the electrochemiluminescence detection cell body 1 near the three-way pipe 14. A second transfer pump 15 for liquid transport is also fixedly connected to the outer wall of the rear end of the electrochemiluminescence detection cell body 1. The input end of the second transfer pump 15 installed on the side wall of the electrochemiluminescence detection cell body 1 is connected to the electrochemiluminescence detection cell body 1. The output end of the second transfer pump 15 installed on the side wall of the electrochemiluminescence detection cell body 1 is fixedly connected to the drain pipe 16 for liquid transport. The output end of the second transfer pump 15 installed on the outer wall of the rear end of the electrochemiluminescence detection cell body 1 is connected to the electrochemiluminescence detection cell body 1. The input end of the second transfer pump 15 installed on the outer wall of the rear end of the electrochemiluminescence detection cell body 1 is fixedly connected to one end of the replenishment pipe 17 for liquid transport. Both the replenishment pipe 17 and the drain pipe 16 are corrugated pipe structures.

[0044] During use, the second transfer pump 15, in conjunction with the drain pipe 16, completes the liquid discharge operation, and the second transfer pump 15, in conjunction with the replenishment pipe 17, completes the replenishment operation, thereby enabling rapid operation according to testing requirements.

[0045] Reference Figure 1 , Figure 2 , Figure 6 and Figure 7 The replenishment tube 17 is fixedly connected to the outside of the outer tube 18, and flexible steel wires 19 are fixedly connected at equal intervals between the inner side wall of the outer tube 18 and the outer side wall of the replenishment tube 17. An abutment block 20 is fixedly connected to one end of the outer tube 18 and one end of the drain tube 16. A plug 21 is fixedly connected to the inside of one end of the abutment block 20. One end of the plug 21 near the side wall of the electrochemiluminescence detection cell body 1 is fixedly connected to one end of the drain tube 16. The replenishment tube 17 is movably connected through the plug 21 near the rear end of the electrochemiluminescence detection cell body 1. A balloon 22 with an inflation structure is fixedly connected to the outer side wall of the plug 21. A silicone sealing gasket 23 is fixedly connected to the outer side wall of the abutment block 20 near the plug 21.

[0046] When replenishing or draining the electrochemiluminescence detection cell body 1 is required, the corresponding operations are completed through the drain pipe 16 and the replenishment pipe 17. Both the drain pipe 16 and the replenishment pipe 17 are inserted into the drain port or the inlet of the storage vessel through the connector 21, so that the contact block 20 abuts and seals. Because the drain pipe 16 and the replenishment pipe 17 are designed with a corrugated pipe structure, they have a high degree of freedom and can be matched with the drain port or the inlet of the storage vessel. The compression balloon 22 abuts against the inner wall of the drain port or the inlet of the storage vessel for limiting the position. During the operation, the replenishment pipe 17 can also be stretched to fit the bottom of the container storing the liquid, thereby reducing the occurrence of residue when replenishing the liquid and ensuring the stability of the replenishment operation. At the same time, the stability of the replenishment pipe 17 is further improved by the cooperation of the outer tube 18 and the flexible steel wire 19.

[0047] The specific operating procedure for the electrochemiluminescence detection cell body 1 is as follows:

[0048] S1: Prepare the electrolyte and luminescent reagent according to the detection requirements and inject them into the electrochemiluminescence detection cell body 1;

[0049] S2: Connect the working electrode, reference electrode, and counter electrode to the electrochemical workstation, and align the optical window 10 with the photodetector;

[0050] S3: Inject blank electrolyte into the detection cell, apply initial voltage, record background luminescence signal, and complete baseline calibration after the signal stabilizes;

[0051] S4: Allow the mixture of the sample to be tested and the luminescent reagent to flow into the detection cell at a uniform rate;

[0052] S5: Under a set voltage, the electrode initiates an electrochemical reaction to generate a light-emitting signal, and the photodetector collects the signal intensity in real time and converts it into data.

[0053] The implementation principle of an improved electrochemiluminescence detection cell structure in this application embodiment is as follows:

[0054] First, when it is necessary to replenish or drain the electrochemiluminescence detection cell body 1, the corresponding operation is completed through the drain pipe 16 and the replenishment pipe 17, and both the drain pipe 16 and the replenishment pipe 17 are connected to the drain port or the inlet of the storage vessel through the connector 21.

[0055] Secondly, the liquid discharge operation is completed by the second transfer pump 15 in conjunction with the drain pipe 16, and the liquid replenishment operation is completed by the second transfer pump 15 in conjunction with the replenishment pipe 17.

[0056] Afterwards, the first transfer pump 11 outputs the work, and then the liquid inside the electrochemiluminescence detection cell body 1 is drawn in from the two three-way pipes 14 and transferred to the inside of the transfer pipe 12. According to the current detection requirements, the return liquid can be heated by the electric heater 13, and the power of the electric heater 13 can be adjusted in real time by the temperature parameter data obtained by the temperature monitoring probe 9.

[0057] Finally, based on the sealing requirements of the electrochemiluminescence detection cell body 1 during use, the sealing cover 2 is used to seal or open the electrochemiluminescence detection cell body 1. The working electrode, reference electrode and counter electrode installed inside the electrochemiluminescence detection cell body 1 are used for detection, and the optical window 10 is used to complete the detection or test. Thus, the improved electrochemiluminescence detection cell structure is completed.

[0058] The above are merely optional embodiments of this disclosure and are not intended to limit this disclosure. Various modifications and variations can be made to this disclosure by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this disclosure should be included within the scope of protection of this disclosure.

Claims

1. An improved electrochemiluminescence detection cell structure, characterized in that: The device includes an electrochemiluminescence detection cell body (1), a sealing cover (2) is vertically and movably connected to the top of the electrochemiluminescence detection cell body (1), a temperature monitoring probe (9) is installed at the bottom of the sealing cover (2), an optical window (10) is installed inside the front end of the electrochemiluminescence detection cell body (1), one side of the electrochemiluminescence detection cell body (1) is connected to the output end of a first transfer pump (11), the input end of the first transfer pump (11) is connected to a transfer tube (12), an electric heater (13) is installed inside the transfer tube (12), and a three-way pipe (14) is installed at the other end of the transfer tube (12). The other two ports of the three-way pipe (14) are connected to the other side of the electrochemiluminescence detection cell body (1).

2. The improved electrochemiluminescence detection cell structure according to claim 1, characterized in that: Mounting plates are installed on both sides of the top of the sealing cover (2), and first connecting brackets (3) are symmetrically installed on the bottom of the mounting plates. The interior of the first connecting brackets (3) is hinged to one end of the connecting arm (4).

3. The improved electrochemiluminescence detection cell structure according to claim 2, characterized in that: The other end of the connecting arm (4) is hinged to the top of the movable block (5), which is movably connected inside the top of the sliding groove (6).

4. The improved electrochemiluminescence detection cell structure according to claim 3, characterized in that: The sliding grooves (6) are symmetrically installed on both outer walls of the electrochemiluminescence detection cell body (1). Male magnetic absorbing plates (7) are symmetrically installed inside the sliding grooves (6), and female magnetic absorbing plates (8) are installed at the corresponding end of the movable block (5).

5. The improved electrochemiluminescence detection cell structure according to claim 1, characterized in that: A second transfer pump (15) is installed on the outer wall of the electrochemiluminescence detection cell body (1) near the three-way pipe (14), and a second transfer pump (15) is also installed on the outer wall of the rear end of the electrochemiluminescence detection cell body (1).

6. The improved electrochemiluminescence detection cell structure according to claim 1, characterized in that: The input end of the second transfer pump (15) installed on the side wall of the electrochemiluminescence detection cell body (1) is connected to the electrochemiluminescence detection cell body (1), and the output end of the second transfer pump (15) installed on the side wall of the electrochemiluminescence detection cell body (1) is connected to the drain pipe (16).

7. The improved electrochemiluminescence detection cell structure according to claim 1, characterized in that: The output end of the second transfer pump (15) installed on the outer wall of the rear end of the electrochemiluminescence detection cell body (1) is connected to the electrochemiluminescence detection cell body (1). The input end of the second transfer pump (15) installed on the outer wall of the rear end of the electrochemiluminescence detection cell body (1) is connected to one end of the replenishment pipe (17). An outer sleeve (18) is installed on the outside of the replenishment pipe (17). Flexible steel wires (19) are installed at equal intervals between the inner side wall of the outer sleeve (18) and the outer side wall of the replenishment pipe (17).

8. The improved electrochemiluminescence detection cell structure according to claim 7, characterized in that: Abutment blocks (20) are installed at one end of the outer sleeve (18) and one end of the drain pipe (16). A connector (21) is installed through the inside of the abutment block (20). One end of the connector (21) near the side wall of the electrochemiluminescence detection cell body (1) is connected to the drain pipe (16). A replenishment pipe (17) is movably connected through the inside of the connector (21) near the rear end of the electrochemiluminescence detection cell body (1). Both the replenishment pipe (17) and the drain pipe (16) are corrugated pipe structures. A balloon (22) is installed on the outer wall of the connector (21). A silicone sealing gasket (23) is adhered to the outer wall of the abutment block (20) near the connector (21).

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