Flow cytometry detection device

Abstract

This utility model relates to the field of cell detection technology and discloses a flow cytometry detection device, including a flow cytometer. A connecting sealed housing is fixedly installed on the right side of the flow cytometer. An installation support is provided inside the connecting sealed housing. A detection station is provided on the top surface of the installation support. A connecting rotation mechanism connected to the detection station is provided inside the installation support. When the drive motor starts working, its drive end drives a pinion to rotate. The pinion drives the detection station to rotate synchronously through meshing with an external gear ring. According to a preset program, the detection station rotates to a target angle, so that the sample carrier (test tube tray or culture plate) to be used is switched to the working position corresponding to the flow cytometer. Another sample carrier is in a pre-loading position. The operator can complete the sample loading in advance in the pre-loading position without waiting for the current detection to end, realizing parallel detection and pre-loading.

Description

Technical Field

[0001] This application belongs to the field of cell detection technology, specifically a flow cytometry detection device. Background Technology

[0002] Flow cytometry is a technique for rapid quantitative analysis and sorting of cells or other biological particles arranged in a single file in a flow stream. Its core is the use of a flow cytometer for detection. This device focuses a sample cell suspension into an extremely fine single-cell stream encapsulated in a sheath fluid, allowing it to pass sequentially at high speed through a laser irradiation zone. When cells carry fluorescent labels and are excited by the laser, they generate scattered light and fluorescence signals. These signals are captured by a series of sophisticated photodetectors (such as photomultiplier tubes, PMTs) and converted into electrical signals. A computer then performs multi-parameter correlation analysis on the various signals from each cell, thereby enabling qualitative and quantitative measurements of cell size, particle size, and surface and internal molecular expression.

[0003] For example, the utility model patent with announcement number CN223244599U discloses an automatic sample loading mechanism for a flow cytometer, including a base plate. A horizontally movable translation plate is connected to the base plate via a translation component. A receiving component that can rotate around its own axis is connected to the translation plate via a rotation component. A material loading component for clamping test tubes is installed on the receiving component. The operating accuracy and efficiency of this mechanism are higher than those of manual operation, which can reduce labor intensity, free manual labor from tedious operations, improve the sample detection efficiency of flow cytometry, and realize rapid sample loading and detection by flow cytometer.

[0004] However, in actual use, it was discovered that the device achieves compatibility between the test tube tray and multi-well culture plates (such as 96-well plates) through an adapter. However, the switching between the test tube tray and culture plate in this device relies on manual disassembly and replacement. This method is time-consuming and labor-intensive, affecting the sample injection efficiency of the flow cytometer. Especially when dealing with large-scale sample testing, the switching time cost will increase significantly. Furthermore, long-term manual disassembly and installation can easily create gaps between related components, which may affect the stability and positioning accuracy of the sample carrier installation, leading to deviations in subsequent sample testing results. Therefore, a flow cytometry detection device is provided. Utility Model Content

[0005] The purpose of this application is to provide a flow cytometry detection device in order to solve the problems mentioned above.

[0006] The technical solution adopted in this application is as follows: a flow cytometry detection device, including a flow cytometer, a connecting sealing shell is fixedly installed on the right side of the flow cytometer, a mounting support is provided inside the connecting sealing shell, a detection fixing stage is provided on the top surface of the mounting support, and a connecting rotation mechanism connected to the detection fixing stage is provided inside the mounting support. The connecting rotation mechanism includes a circular connecting rod, a spline groove, a spline shaft, an external gear ring, a drive motor, and a pinion. The circular connecting rod is fixedly mounted on the bottom surface of the detection station. The bottom end of the circular connecting rod extends into the interior of the mounting bracket. A spline groove is formed at one end of the circular connecting rod extending into the interior of the mounting bracket. A spline shaft corresponding to the circular connecting rod is rotatably connected inside the mounting bracket. The top end of the spline shaft is inserted into the spline groove. An external gear ring is fixedly mounted on the outer surface of the spline shaft. A drive motor is fixedly mounted on the top surface inside the mounting bracket. A pinion gear is fixedly mounted on the drive end of the drive motor. The pinion gear meshes with the external gear ring.

[0007] In a preferred embodiment, a T-shaped slot is provided on the inner bottom surface of the connecting sealing housing, and a plug is inserted into the T-shaped slot. The top end of the plug is fixed to the bottom surface of the mounting bracket.

[0008] In a preferred embodiment, the side of the connecting sealed housing away from the flow cytometer is hinged with a sealed door via a hinge.

[0009] In a preferred embodiment, a bearing housing is fixedly mounted on the inner bottom surface of the mounting bracket, and the bottom end of the spline shaft is fixedly mounted in the middle of the bearing housing.

[0010] In a preferred embodiment, an adsorption magnetic sheet is fixedly installed at one end of the insert block, and an iron sheet corresponding to the adsorption magnetic sheet is provided at one end of the T-shaped slot, and the adsorption magnetic sheet and the iron sheet are attracted to each other.

[0011] In a preferred embodiment, the flow cytometer is fixedly fitted with multiple rubber anti-slip feet near the corners of its bottom surface.

[0012] In summary, due to the adoption of the above technical solution, the beneficial effects of this application are: 1. In this application, due to the adoption of the above-mentioned scheme, after the detection station is installed, one of the test tube trays and culture plates on the detection station is located in the working position below the flow cytometer detection needle, and the other is in the waiting position. Then, the detection needle on the flow cytometer moves under the drive of the X, Y and Z moving mechanisms to sample and detect the test tube tray or culture plate in the working position. After the test tube tray or culture plate in the working position has been sampled, the drive motor starts working, and its drive end drives the pinion to rotate. The pinion drives the detection station to rotate synchronously through the meshing transmission with the external gear ring. According to the preset program, the detection station rotates to the target angle, so that the sample carrier (test tube tray or culture plate) to be used is switched to the working position corresponding to the flow cytometer, and the other sample carrier is in the pre-loading position. The operator can complete the sample loading in advance in the pre-loading position without waiting for the current detection to end, realizing parallel detection and pre-loading, eliminating the need for manual disassembly and replacement of carriers, ensuring the stability of the detection process, and effectively improving the overall detection efficiency and accuracy. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this application; Figure 2 This is a schematic diagram of the internal structure of the connection sealing housing in this application; Figure 3 This is a schematic diagram of the testing station structure in this application; Figure 4 This is a schematic diagram of the circular connecting rod structure of this application; Figure 5 This is a schematic diagram of the insert structure of this application.

[0014] The diagram shows: 1. Flow cytometer; 2. Connecting sealed housing; 3. Mounting support; 4. Detection station; 5. Connecting rotating mechanism; 501. Circular connecting rod; 502. Spline groove; 503. Spline shaft; 504. External gear ring; 505. Drive motor; 506. Pinion; 6. T-slot; 7. Insert block; 8. Sealed door; 9. Bearing seat; 10. Adsorption magnetic sheet; 11. Rubber anti-slip feet. Detailed Implementation

[0015] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below in conjunction with the embodiments of this application. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments in this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0016] refer to Figures 1-5As shown, the flow cytometry detection device includes a flow cytometer 1. Multiple rubber anti-slip feet 11 are fixedly installed on the bottom surface of the flow cytometer 1 near the corners. The rubber anti-slip feet 11 increase the friction between the flow cytometer 1 and the placement surface, preventing displacement of the device due to mechanical vibration (such as the operation of internal drive components) during operation, ensuring stable detection position. At the same time, the rubber material has a buffering effect, absorbing the vibration transmitted to the flow cytometer 1 from the outside, reducing the impact of vibration on internal precision detection components (such as optical system and sampling mechanism), and reducing detection error.

[0017] refer to Figures 1-5 As shown, a connecting sealing housing 2 is fixedly installed on the right side of the flow cytometer 1. A sealing door 8 is hinged to the side of the connecting sealing housing 2 away from the flow cytometer 1 via a hinge. An installation support 3 is provided inside the connecting sealing housing 2. A T-shaped slot 6 is formed on the bottom surface of the connecting sealing housing 2. A plug 7 is inserted into the T-shaped slot 6, with its top fixed to the bottom surface of the installation support 3. An adsorption magnetic sheet 10 is fixedly installed at one end of the plug 7. An iron sheet corresponding to the adsorption magnetic sheet 10 is provided at one end of the T-shaped slot 6, and the adsorption magnetic sheet 10 is attracted to the iron sheet. The connecting sealing housing 2 forms a closed space, protecting the internal installation support 3 and subsequent sample carriers. To protect test tubes and culture plates from external dust and impurities, and to prevent cross-contamination of samples during testing due to external airflow interference, the sealed door 8 is hinged for easy opening by operators for sample pre-loading and component maintenance. Closing it further enhances the sealing effect. The T-slot 6 and the plug 7 are connected to achieve quick positioning and installation of the mounting bracket 3, which can be disassembled and assembled without complicated tools, improving maintenance efficiency. The magnetic adsorption sheet 10 and the iron sheet are attracted to each other, which further fixes the relative position of the plug 7 and the T-slot 6, preventing the mounting bracket 3 from loosening or shifting due to vibration during device operation, thus ensuring the accuracy of sample testing.

[0018] refer to Figures 1-5As shown, a detection fixing platform 4 is provided on the top surface of the mounting support 3, and a connecting rotation mechanism 5 connected to the detection fixing platform 4 is provided inside the mounting support 3. Test tube trays and culture plates are respectively provided on the top surface of the detection fixing platform 4. The detection fixing platform 4 provides a stable mounting platform for sample carriers (test tube trays and culture plates), ensuring that the sample carriers are fixed in position during the detection process, which facilitates accurate sampling by the flow cytometer 1. The test tube trays and culture plates can be installed by fixed installation or by fitting and plugging in grooves and protrusions. The connecting rotation mechanism 5 can drive the detection fixing platform 4 to rotate, realizing the rapid switching of different sample carriers (such as test tube trays and culture plates) without the need for manual disassembly and replacement of carriers, reducing operation time, improving detection efficiency, and avoiding the component gap problem caused by frequent disassembly and assembly, thus ensuring the positioning accuracy for long-term use.

[0019] refer to Figures 1-5 As shown, the connecting rotation mechanism 5 includes a circular connecting rod 501, a spline groove 502, a spline shaft 503, an external gear ring 504, a drive motor 505, and a pinion 506. The circular connecting rod 501 is fixedly mounted on the bottom surface of the detection station 4. The bottom end of the circular connecting rod 501 extends into the interior of the mounting support 3. A spline groove 502 is formed at one end of the circular connecting rod 501 extending into the mounting support 3. A spline shaft 503 corresponding to the circular connecting rod 501 is rotatably connected inside the mounting support 3. A bearing seat 9 is fixedly mounted on the bottom surface of the mounting support 3. The bottom end of the spline shaft 503 is fixedly mounted in the middle of the bearing seat 9, and the top end of the spline shaft 503 is inserted into the spline groove 502. Internally, the splined shaft 503 is connected to the circular connecting rod 501 via a spline groove 502. The spline connection features high transmission torque and precise positioning, ensuring that the rotational power of the splined shaft 503 is stably transmitted to the testing station 4, preventing slippage and free rotation, and ensuring the precise rotation angle of the testing station 4. The bearing seat 9 provides stable support for the splined shaft 503, reducing radial runout during rotation, lowering frictional losses, and extending the service life of the splined shaft 503. At the same time, it further improves the stability of the rotation of the testing station 4, preventing sample carrier displacement due to shaft wobbling. In addition, the spline connection facilitates the installation and disassembly of the testing station 4.

[0020] refer to Figures 1-5As shown, an external gear ring 504 is fixedly mounted on the outer surface of the spline shaft 503, and a drive motor 505 is fixedly mounted on the inner top surface of the mounting bracket 3. A pinion 506 is fixedly mounted on the drive end of the drive motor 505, and the pinion 506 meshes with the external gear ring 504. The drive motor 505 transmits power to the spline shaft 503 through the meshing of the pinion 506 and the external gear ring 504. The gear transmission has the advantages of high transmission efficiency and precise speed control, which can realize the precise control of the rotation speed and angle of the detection station 4, and meet the position requirements when switching different sample carriers.

[0021] The implementation principle of the flow cytometry detection device embodiment of this application is as follows: The user first installs the test tube tray and culture plate on the detection station 4, then opens the sealing box door 8 on the connecting sealing housing 2, and inserts the plug 7 on the bottom surface of the mounting support 3 into the T-shaped slot 6 on the bottom surface of the connecting sealing housing 2 until the magnetic adsorption sheet 10 of the plug 7 is adsorbed and fixed with the iron sheet in the T-shaped slot 6, thus completing the positioning and installation of the mounting support 3. Then the sealing box door 8 is closed to form a closed protective space. At this time, on the test tube tray and culture plate on the detection station 4, one is in the working position below the detection needle of the flow cytometer 1, and the other is in the waiting position. Then, the detection needle on the flow cytometer 1 moves under the drive of the X, Y and Z movement mechanism to sample and detect the test tube tray or culture plate in the working position. After the test tube tray or culture plate in the working position has been sampled, the drive motor 505 starts working, and its drive end drives the pinion 506 to rotate. The pinion 506 drives the spline shaft 503 to rotate smoothly under the support of the bearing seat 9 through the meshing transmission with the external gear ring 504. When the spline shaft 503 rotates, it connects with the circular connecting rod 5 through the spline groove 502. The spline mechanism of 01 drives the detection stage 4 to rotate synchronously. According to the preset program, the detection stage 4 rotates to the target angle, so that the sample carrier (test tube tray or culture plate) to be used is switched to the working position corresponding to the flow cytometer 1, while the other sample carrier is in the pre-loading position. The operator can complete the sample loading in advance in the pre-loading position without waiting for the current test to end, realizing parallel detection and pre-loading. There is no need to manually disassemble and change the carrier. The automatic rotation of the connecting rotating mechanism 5 realizes rapid switching. At the same time, the sealing protection of the connecting sealed shell 2 and the precise transmission of each component ensure that the detection process is stable and pollution-free, effectively improving the overall detection efficiency and accuracy.

[0022] The above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. A flow cytometry detection device comprising a flow cytometer (1), characterized in that: A connecting sealing housing (2) is fixedly installed on the right side of the flow cytometer (1). A mounting support (3) is provided inside the connecting sealing housing (2). A detection fixing stage (4) is provided on the top surface of the mounting support (3). A connecting rotation mechanism (5) connected to the detection fixing stage (4) is provided inside the mounting support (3). The connecting rotation mechanism (5) includes a circular connecting rod (501), a spline groove (502), a spline shaft (503), an external gear ring (504), a drive motor (505), and a pinion (506). The circular connecting rod (501) is fixedly installed on the bottom surface of the detection fixing table (4). The bottom end of the circular connecting rod (501) extends into the interior of the mounting support (3). A spline groove (502) is provided at one end of the circular connecting rod (501) extending into the interior of the mounting support (3). (3) is internally rotatably connected to a spline shaft (503) corresponding to a circular connecting rod (501). The top end of the spline shaft (503) is inserted into the spline groove (502). An external gear ring (504) is fixedly installed on the outer surface of the spline shaft (503). A drive motor (505) is fixedly installed on the inner top surface of the mounting bracket (3). A pinion (506) is fixedly installed on the drive end of the drive motor (505). The pinion (506) meshes with the external gear ring (504).

2. The flow cytometry assay device of claim 1, wherein: The inner bottom surface of the connecting sealing housing (2) is provided with a T-shaped slot (6), and a plug (7) is inserted into the T-shaped slot (6). The top of the plug (7) is fixed to the bottom surface of the mounting bracket (3).

3. The flow cytometry apparatus of claim 1, wherein: The sealing housing (2) is connected to a sealed door (8) on the side away from the flow cytometer (1) by a hinge.

4. The flow cytometry apparatus of claim 1, wherein: The bearing seat (9) is fixedly installed on the inner bottom surface of the mounting bracket (3), and the bottom end of the spline shaft (503) is fixedly installed in the middle of the bearing seat (9).

5. The flow cytometry apparatus of claim 2, wherein: One end of the insert (7) is fixedly equipped with an adsorption magnetic sheet (10), and one end of the T-shaped slot (6) is provided with an iron sheet corresponding to the adsorption magnetic sheet (10), and the adsorption magnetic sheet (10) is attracted to the iron sheet.

6. The flow cytometry apparatus of claim 1, wherein: The flow cytometer (1) has multiple rubber anti-slip feet (11) fixedly installed on the bottom surface near the corners.

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