A detachable culture device for plant tissue culture

Abstract

The utility model discloses a detachable culture device for plant tissue culture relates to plant culture technical field. Including culture dish body and casing, the culture dish body is set up and can be split into a plurality of independent culture unit through cutting tool, the culture unit forms the culture chamber of top opening, the culture chamber is used for cultivating plant tissue, and the culture dish body is formed to culture dish body through the connection of the connecting wall between a plurality of culture chamber, the casing forms the cup type structure of top opening of wide top narrow, and the inner wall of casing and the edge of culture dish body are abutted, and the casing is used for accommodating and fixing culture dish body. The utility model provides a culture device for improving plant tissue culture efficiency, realizes mechanization, intelligent culture production.

Description

Technical Field

[0001] This utility model relates to the field of plant culture technology, and in particular to a detachable culture device for plant tissue culture. Background Technology

[0002] Plant tissue culture is a new asexual reproduction technique developed based on the theory of plant cell totipotency. In a broad sense, plant tissue culture, also known as in vitro culture, refers to the technique of isolating desired tissues, organs, cells, or protoplasts from a plant, aseptically inoculating them onto a culture medium containing nutrients and plant hormones, and culturing them under aseptic conditions to obtain regenerated complete plants or produce economically valuable products. In a narrow sense, it specifically refers to the technique of using various plant tissues (such as cambium, parenchyma, mesophyll, endosperm, etc.) to obtain regenerated plants, or inducing callus tissue from various organs during the culture process, which then redifferentiates to form regenerated plants.

[0003] In current technological practices, plant tissue culture faces numerous challenges in large-scale, factory-like, and intelligent production. Taking traditional culture containers as an example, the roots of tissue culture seedlings easily become entangled during growth. When the seedlings reach the shipping standard and need to be sorted, they can only be separated manually. This process not only reduces efficiency but also easily damages the plant roots with slight carelessness, affecting the subsequent growth and survival rate of the seedlings. More importantly, this manual separation method is difficult to mechanize, severely hindering the transformation of plant tissue culture towards a mechanized and intelligent production model, and impeding the large-scale and efficient development of the industry. Summary of the Invention

[0004] The purpose of this invention is to provide a detachable culture device for plant tissue culture, which can improve the efficiency of plant tissue culture and realize mechanized and intelligent culture production.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a detachable culture device for plant tissue culture, comprising a culture disc and a shell, wherein the culture disc is configured to be detachable into multiple independent culture units by a cutting tool, each culture unit forming a culture chamber with a top opening, the culture chamber being used for culturing plant tissue, and multiple culture chambers being connected by a connecting wall to form the culture disc; the shell is formed into a cup-shaped structure with a wider top and narrower bottom and a top opening, and the inner wall of the shell abuts against the edge of the culture disc, the shell being used to accommodate and fix the culture disc.

[0006] Optionally, the plurality of culture units are configured to be distributed in an array-like equally divided structure.

[0007] Optionally, the housing is configured as a cuboid structure, and a strip groove is provided on the outer side wall of the housing, the strip groove being used for gripping by workers or robotic arms.

[0008] Optionally, the strip grooves are spaced apart along the height direction of the housing, and the number of strip grooves is at least two sets.

[0009] Optionally, the culture device further includes a sealing film disposed on the top of the shell, the sealing film having a breathable membrane for gas exchange between the culture environment and the outside.

[0010] Optionally, the sealing film is made of PE / PP or other materials, the breathable membrane is made of PTFE, and the breathable membrane is located at other positions on the sealing film except for the center.

[0011] Optionally, the culture tray is integrally injection molded from corrosion-resistant and biocompatible materials such as PE or PP.

[0012] Compared with existing technologies, the detachable culture device for plant tissue culture provided by this utility model features independent culture units and a detachable design for the culture tray, allowing each tissue culture seedling to grow in a separate chamber, completely avoiding root entanglement and eliminating the need for manual stripping during transplanting, thus significantly reducing root damage. Simultaneously, the independent chambers prevent the spread of pathogens in the culture medium, and combined with the aseptic barrier function of the PTFE breathable membrane, the cross-infection rate is significantly reduced. Furthermore, the strip-shaped grooves on the shell allow for both manual and mechanical gripping, with a very high success rate for robotic arm gripping. This can replace manual labor in core steps such as culture medium inoculation, container handling, and culture tray disassembly, achieving mechanized and intelligent operation, which is conducive to promoting the transformation of plant tissue culture towards industrialization and intelligentization. Attached Figure Description

[0013] Figure 1 A schematic diagram of the structure of the culture device provided in the embodiment of this utility model.

[0014] Figure 2 This is a schematic diagram of the structure of the culture disc provided in an embodiment of the present invention.

[0015] Figure 3 A schematic diagram of the structure of the housing provided in an embodiment of this utility model.

[0016] Figure 4 A partial structural schematic diagram of the culture device provided in this application.

[0017] Reference numerals: 100-Cultivation device; 1-Cultivation tray; 2-Cultivation unit; 3-Shell; 4-Strip groove; 5-Sealing film; 6-Ventilating film. Detailed Implementation

[0018] To make the technical problems, technical solutions, and beneficial effects of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0019] It should be noted that when a component is referred to as being "fixed to" or "set on" another component, it can be directly on or indirectly on that other component. When a component is referred to as being "connected to" another component, it can be directly connected to or indirectly connected to that other component.

[0020] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified. "Several" means one or more, unless otherwise explicitly specified.

[0021] In the description of this utility model, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0022] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0023] Please see Figures 1-4The detachable culture device 100 for plant tissue culture provided in this embodiment of the present invention includes a culture disc 1 and a shell 3. The culture disc 1 is configured to be detachable into multiple independent culture units 2 by a cutting tool. Each culture unit 2 forms a culture chamber with a top opening. The culture chamber is used to culture plant tissue. Multiple culture chambers are connected by a connecting wall to form the culture disc 1. The shell 3 is formed into a cup-shaped structure with a top opening that is wider at the top and narrower at the bottom. The inner wall of the shell 3 abuts against the edge of the culture disc 1. The shell 3 is used to accommodate and fix the culture disc 1.

[0024] Each culture unit 2 has an internal culture chamber for containing culture medium and plant tissue. The chamber space can be flexibly designed according to the growth cycle of tissue culture seedlings to ensure that the roots have sufficient space to grow.

[0025] When the tissue culture seedlings reach the transplanting standard, the culture tray 1 can be separated into individual independent culture units 2 by cutting along the conjoined wall using laser cutting or mechanical milling tools. This eliminates the need for manual root removal and completely solves the problem of root entanglement. In addition, the conjoined structure maintains overall stability during the early stages of culture and transportation, preventing culture medium spillage.

[0026] In this application, the multiple culture units 2 are arranged in an array-like, equally spaced structure. The multiple culture units 2 are arranged in arrays of 3×3, 4×4, 5×5, etc., with uniform spacing between adjacent culture units 2. This ensures that the environmental conditions such as light and temperature received by each unit are consistent, and also facilitates standardized operation by automated equipment, avoiding positional deviations caused by manual inoculation.

[0027] In one embodiment provided in this application, the housing 3 is configured as a cuboid structure that is wider at the top and narrower at the bottom, and a strip groove 4 is provided on the outer side wall of the housing 3, the strip groove 4 being used for gripping by workers or robotic arms.

[0028] The shell 3 has an opening at the top and has an overall rectangular structure that is wider at the top and narrower at the bottom. Compared with traditional cylindrical containers, the rectangular structure with a wider top and narrower bottom has the following advantages: First, it has higher stacking stability, allowing for multi-layer stacking and storage, which greatly improves the space utilization of the culture room and also facilitates large-scale intelligent production; Second, it is easy to adapt to the standardized dimensions of culture racks and sterilization equipment without the need for additional customized special equipment.

[0029] The internal dimensions of the shell 3 are precisely matched with the specifications of the culture tray 1: When the integral culture tray 1 is placed, the gap between the inner wall of the shell 3 and the edge of the culture tray 1 is extremely small, which prevents the tray from shaking inside the shell 3 and causing the culture medium to spill; when the separate independent culture units 2 are placed, they can be placed in single wells or in multi-well arrays as needed, adapting to different transplanting scenarios and improving the versatility of the device.

[0030] Furthermore, the strip grooves 4 are spaced apart along the height direction of the housing 3, and the number of strip grooves 4 is at least two sets.

[0031] The outer wall of the housing 3 is provided with a recessed strip groove 4 that extends into the housing. At least two sets of the strip groove 4 are spaced apart along the height direction of the housing 3. The size design of the strip groove 4 can take into account both manual and mechanical operation needs. That is, the width and depth can be adapted to the grip of an adult's fingers, making it easy for workers to hold it stably. At the same time, it can facilitate the precise matching of the gripping claws of mechanical grippers (such as clamping claws or suction cups). After the gripping claws are inserted into the grooves, they can stably clamp the housing 3, preventing it from slipping during transportation, and significantly improving operational safety and efficiency.

[0032] In addition, when transferring the culture trays inside the shell to the outside, the mechanical gripper used to grasp the culture trays can extend along the gaps in the inner wall of the shell to the lower edge of the culture trays. After the mechanical gripper grasps the edge of the culture trays, it lifts the culture trays out of the shell by lifting them upwards.

[0033] In this application, the culture device 100 further includes a sealing film 5 disposed on the top of the shell 3, and the sealing film 5 is provided with a breathable membrane 6 for gas exchange between the culture environment and the outside world.

[0034] Furthermore, the sealing film 5 is made of PE / PP or similar material, and the breathable membrane 6 is made of PTFE, with the breathable membrane 6 positioned at all locations except the center of the sealing film 5. The PTFE membrane possesses excellent breathability and corrosion resistance, effectively ensuring gas exchange between the cultivation environment and the external environment while preventing the entry of external impurities, bacteria, and microorganisms. Positioning the breathable membrane 6 at all locations except the center of the sealing film 5 ensures gas exchange while also preventing interference during subsequent transport using a suction cup to adhere to the center of the sealing film 5.

[0035] In this application, the culture tray 1 is integrally injection molded from corrosion-resistant and biocompatible materials such as PE or PP. This method ensures that the culture tray 1 has a complete and seamless structure, reducing gaps for microbial attachment and growth, thus lowering the risk of contamination. Furthermore, it facilitates mass production, significantly reducing mold costs compared to traditional modular culture cups, making it more suitable for industrial applications.

[0036] As can be seen from the structure of the above-mentioned culture device 100, the independent culture unit 2 of the culture tray 1 and its detachable design allow each tissue culture seedling to grow in a separate chamber, completely avoiding root entanglement. No manual peeling is required during transplanting, and the root damage rate is greatly reduced. At the same time, the independent chambers block the spread of pathogens in the culture medium. Combined with the aseptic barrier function of the PTFE breathable membrane 6, the cross-infection rate is significantly reduced. In addition, the strip groove 4 of the shell 3 is adapted for both manual and mechanical grasping. The robotic arm has a very high grasping success rate and can replace manual labor in completing core steps such as culture medium inoculation, container handling, and disassembly of the culture tray 1, realizing mechanized and intelligent operation, which is conducive to promoting the transformation of plant tissue culture towards industrialization and intelligence.

[0037] In the description of the above embodiments, specific features, structures, materials, or characteristics may be combined in any suitable manner in one or more embodiments or examples.

[0038] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A detachable culture device for plant tissue culture, characterized in that, The device includes a culture tray and a shell. The culture tray is designed to be disassembled into multiple independent culture units by a cutting tool. Each culture unit forms a culture chamber with a top opening. The culture chamber is used to culture plant tissues. Multiple culture chambers are connected by a connecting wall to form the culture tray. The shell is formed into a cup-shaped structure with a wider top and a narrower bottom and a top opening. The inner wall of the shell abuts against the edge of the culture tray. The shell is used to accommodate and fix the culture tray.

2. The culture apparatus according to claim 1, characterized in that, The multiple culture units are arranged in an array-like, equally divided structure.

3. The culture apparatus according to claim 1, characterized in that, The shell is configured as a cuboid structure, and a strip-shaped groove is provided on the outer side wall of the shell, which is used for workers or robotic arms to grasp.

4. The culture apparatus according to claim 3, characterized in that, The strip grooves are spaced apart along the height direction of the housing, and the number of the strip grooves is at least two sets.

5. The culture apparatus according to claim 1, characterized in that, The culture device also includes a sealing film disposed on the top of the shell, and the sealing film is provided with a breathable membrane for gas exchange between the culture environment and the outside world.

6. The culture apparatus according to claim 5, characterized in that, The sealing film is made of PE / PP or similar material, the breathable membrane is made of PTFE, and the breathable membrane is located at positions other than the center of the sealing film.

7. The culture apparatus according to claim 1, characterized in that, The culture tray is made of corrosion-resistant and biocompatible materials such as PE or PP through integrated injection molding.

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