A tool for rapid preparation of polyacrylamide gels

Abstract

The utility model relates to polyacrylamide gel preparation technical field discloses a kind of tools for rapidly preparing polyacrylamide gel, including batten;One side of batten integrally formed connection has U frame board, the top of U frame board is installed with transverse linear motor, the translation movable end of transverse linear motor is fixed with longitudinal linear motor, the lifting end of longitudinal linear motor is installed with reciprocating drive structure, the movable end of reciprocating drive structure is fixed with semicircular rubber head.The tool for rapidly preparing polyacrylamide gel, by the semicircular rubber head of high-frequency reciprocating movement constantly from one side rubber glass outer side applies controllable knock or vibration, vibration significantly reduces the local viscous resistance of glue liquid, so that it can be evenly filled in short time entire mould space, and, by transverse linear motor and longitudinal linear motor can the horizontal, longitudinal position where semicircular rubber head is located is adjusted to different degree, so that it can knock at different positions of rubber glass.

Description

Technical Field

[0001] This utility model relates to the field of polyacrylamide gel preparation technology, and in particular to a tool for rapidly preparing polyacrylamide gel. Background Technology

[0002] Polyacrylamide gel electrophoresis is an indispensable key technique in fields such as biochemistry and molecular biology, and is widely used for the separation, analysis, and identification of biomolecules such as proteins and nucleic acids. In the implementation of this technique, preparing high-quality polyacrylamide gels is the first and crucial step.

[0003] In the traditional gel preparation process, the gel solution needs to be injected into a slit mold consisting of two parallel gel-making glass slides, and the gel solution will naturally diffuse and fill the gaps through capillary action or gravity.

[0004] When the adhesive is introduced into the narrow gap between two adhesive glass slides, due to the extremely limited space of the cavity and the viscosity of the adhesive itself, the adhesive mainly relies on gravity and capillary action to diffuse, which is slow. Especially in areas far from the dispensing port, the flow and diffusion of the adhesive in the cavity are severely hindered, and incomplete filling or uneven distribution of adhesive is likely to occur in the corners, edges, or locations far from the dispensing point. Utility Model Content

[0005] In view of the problem that the corners, edges or locations far from the injection point of the existing cavity are prone to incomplete filling or uneven distribution of adhesive, this utility model is proposed.

[0006] Therefore, the purpose of this invention is to provide a tool for rapidly preparing polyacrylamide gel, the purpose of which is to accelerate the diffusion of the gel by tapping.

[0007] To solve the above technical problems, the present invention provides the following technical solution: a tool for rapidly preparing polyacrylamide gel, comprising a strip plate, wherein a clamping assembly is fixed to the top of the strip plate, and clamping strips are fixed to the top of both movable ends of the clamping assembly;

[0008] A U-frame plate is integrally formed and connected to one side of the strip plate. A horizontal linear motor is installed on the top of the U-frame plate. A vertical linear motor is fixed to the translating movable end of the horizontal linear motor. A reciprocating drive structure is installed on the lifting end of the vertical linear motor. A semi-circular rubber head is fixed to the movable end of the reciprocating drive structure.

[0009] As an improved technical solution, the reciprocating drive structure includes a lifting chamber fixed on the lifting end of a longitudinal linear motor. A positioning sleeve is welded to the side of the lifting chamber near the strip. A movable column is laterally slidably installed inside the positioning sleeve, and one end of the movable column is located outside the positioning sleeve and is fixedly connected to a semi-circular rubber head. A drive body for driving the movable column to move laterally is installed inside the lifting chamber.

[0010] As an improved technical solution, slide chambers are fixed on both sides inside the positioning sleeve, and guide strips that slide inside the slide chambers are installed on both sides of the periphery of the movable column.

[0011] As an improved technical solution, the driving body includes a second servo motor fixed inside the lifting chamber on the side away from the movable column, and a U-shaped frame installed on the end of the movable column away from the semi-circular rubber head. The driving end of the second servo motor is fixedly connected to a rotating disk. An installation shaft is rotatably installed on the outer edge of the rotating disk away from the second servo motor. A push-pull bar is rotatably connected to the installation shaft, and the end of the push-pull bar away from the installation shaft is rotatably installed inside the U-shaped frame.

[0012] As an improved technical solution, the clamping assembly includes a bidirectional lead screw rotatably mounted on the top of the strip via a bearing. Both sides of the bidirectional lead screw are threaded with transmission bars, and the transmission bars are laterally slidably mounted on the top of the strip. The clamping bar is fixed to the top of the transmission bars and to one side near the middle.

[0013] As an improved technical solution, a first servo motor for driving the bidirectional lead screw to rotate is installed on one side of the top of the strip, and a threaded hole for threaded connection with the bidirectional lead screw is started in the middle of the transmission bar. Two guide rails are installed on the top of the strip, and sliders that slide on the guide rails are installed at both ends of the bottom of the transmission bar.

[0014] After adopting the above technical solution, the beneficial effects of this utility model are:

[0015] 1. This utility model allows for quick assembly, disassembly, and replacement of the adhesive-clamping frame by adjusting the spacing between the two clamping strips via a threaded drive. Furthermore, adjusting the spacing between the two clamping strips also adapts to clamping operations on adhesive-clamping frames of different sizes, improving its adaptability. Moreover, fixing the adhesive-clamping frame, i.e., fixing the adhesive-forming glass slide, eliminates the need for manual hand-held fixing, improving convenience while ensuring the stability of the semi-circular rubber head when striking the adhesive-forming glass slide.

[0016] 2. In this utility model, the horizontal linear motor can adjust the horizontal position of the semi-circular rubber head, and the vertical linear motor can adjust only the vertical height of the semi-circular rubber head. This allows the semi-circular rubber head to be adjusted to different positions on the adhesive-making glass slide, expanding the range of impact on the glass slide and facilitating the rapid, comprehensive, and uniform diffusion of the adhesive between the two glass slides.

[0017] 3. This invention utilizes a high-frequency reciprocating semi-circular rubber head to continuously apply controllable tapping or vibration from the outside of the gel-forming glass slide on one side. The generated mechanical waves can be effectively transmitted to the adhesive within the cavity. The vibration significantly reduces the local viscous resistance of the adhesive and disrupts the static friction within the adhesive, thereby greatly promoting the rapid flow and diffusion of the adhesive within the narrow cavity. This allows the adhesive to uniformly fill the entire mold space in a short time, while effectively eliminating air bubbles and improving gel quality. Furthermore, the horizontal and vertical linear motors can adjust the horizontal and vertical positions of the semi-circular rubber head to different degrees, allowing it to tap different locations on the gel-forming glass slide. This facilitates rapid and comprehensive filling of the adhesive, avoiding localized concentration differences or uneven curing, and ensuring a high degree of uniformity in the internal structure of the gel. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Among them:

[0019] Figure 1 This is a schematic diagram of the overall structure of a tool for rapidly preparing polyacrylamide gel according to the present invention.

[0020] Figure 2 This is a cross-sectional schematic diagram of the reciprocating drive structure of a tool for rapidly preparing polyacrylamide gel according to this utility model.

[0021] Figure 3 This is a rear view schematic diagram of the rotating disk of the tool for rapidly preparing polyacrylamide gel according to this utility model.

[0022] Explanation of reference numerals in the attached figures:

[0023] 1. Strip plate; 2. U-shaped frame plate; 3. Horizontal linear motor; 4. Vertical linear motor; 5. Reciprocating drive structure; 51. Lifting chamber; 52. Rotary disk; 53. Mounting shaft; 54. Push-pull bar; 55. U-shaped frame; 56. Positioning sleeve; 57. Slide chamber; 58. Movable column; 59. Guide bar; 510. Second servo motor; 6. Semi-circular rubber head; 7. Clamping assembly; 71. First servo motor; 72. Bidirectional lead screw; 73. Transmission bar; 74. Guide rail; 75. Slider; 8. Clamping bar. Detailed Implementation

[0024] To make the above-mentioned objectives, features and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings.

[0025] Example 1

[0026] Reference Figures 1-3 This is the first embodiment of the present invention, which provides a tool for rapidly preparing polyacrylamide gel. This tool for rapidly preparing polyacrylamide gel includes a strip plate 1, a clamping assembly 7 fixed to the top of the strip plate 1, and clamping strips 8 fixed to the top of the two movable ends of the clamping assembly 7.

[0027] A U-shaped frame plate 2 is integrally formed on one side of the strip plate 1, and a weight-reducing cavity is formed between the strip plate 1 and the U-shaped frame plate 2. A horizontal linear motor 3 is installed on the top of the U-shaped frame plate 2. A vertical linear motor 4 is fixed to the movable end of the horizontal linear motor 3. A reciprocating drive structure 5 is installed on the lifting end of the vertical linear motor 4. A semi-circular rubber head 6 is fixed to the movable end of the reciprocating drive structure 5. The semi-circular rubber head 6 is made of rubber to reduce the damage caused by striking the glue-making glass slide. The horizontal position of the semi-circular rubber head 6 can be adjusted by the horizontal linear motor 3, and the vertical linear motor 4 can adjust only the vertical height of the semi-circular rubber head 6. This allows the semi-circular rubber head 6 to be adjusted to different positions on the glue-making glass slide, expanding the range of striking the glue-making glass slide and making it easier for the glue to spread quickly, comprehensively and evenly between the two glue-making glass slides.

[0028] The reciprocating drive structure 5 includes a lifting chamber 51 fixed on the lifting end of the longitudinal linear motor 4. A positioning sleeve 56 is welded to the side of the lifting chamber 51 near the strip 1, and the interior of the lifting chamber 51 is connected to the interior of the positioning sleeve 56. A movable column 58 is slidably installed inside the positioning sleeve 56, and one end of the movable column 58 is located outside the positioning sleeve 56 and is fixedly connected to the semi-circular rubber head 6. A drive body for driving the movable column 58 to move laterally is installed inside the lifting chamber 51, and the drive end of the drive body is fixed to the other end of the movable column 58.

[0029] Both sides of the positioning sleeve 56 are fixed with slide chambers 57, and both sides of the periphery of the movable column 58 are equipped with guide strips 59 that slide within the slide chambers 57.

[0030] The drive unit includes a second servo motor 510 fixed inside the lifting chamber 51 on the side away from the movable column 58, and a U-shaped frame 55 installed on the end of the movable column 58 away from the semi-circular rubber head 6. The drive end of the second servo motor 510 is fixedly connected to a rotating disk 52. An installation shaft 53 is rotatably installed on the outer edge of the end of the rotating disk 52 away from the second servo motor 510. A push-pull strip 54 is rotatably connected to the installation shaft 53, and the end of the push-pull strip 54 away from the installation shaft 53 is rotatably installed inside the U-shaped frame 55.

[0031] During use, the clamping component 7 fixes and limits the glass slide for gel preparation. The semi-circular rubber head 6, which moves at a high frequency, continuously applies controllable tapping or vibration from the outside of the glass slide on one side. The mechanical waves generated can be effectively transmitted to the adhesive in the cavity. The vibration significantly reduces the local viscous resistance of the adhesive and destroys the static friction inside the adhesive, thereby greatly promoting the rapid flow and diffusion of the adhesive in the narrow cavity. This allows the adhesive to fill the entire mold space evenly in a short time. At the same time, it can effectively eliminate air bubbles and improve the gel quality. Furthermore, the horizontal linear motor 3 and the vertical linear motor 4 can adjust the horizontal and vertical positions of the semi-circular rubber head 6 to different degrees, allowing it to tap different positions on the glass slide for gel preparation. This facilitates rapid and comprehensive filling of the adhesive, avoids problems such as local concentration differences or uneven curing, and ensures the high uniformity of the internal structure of the gel.

[0032] Example 2

[0033] Reference Figure 1 This is the second embodiment of the present invention. The difference between this embodiment and the first embodiment is that the clamping assembly 7 includes a bidirectional lead screw 72 that is rotatably mounted on the top of the strip plate 1 via a bearing. Both sides of the bidirectional lead screw 72 are threaded with transmission bars 73, and the transmission bars 73 are laterally slidably mounted on the top of the strip plate 1. The clamping bar 8 is fixed on the top of the transmission bar 73 and on one side near the middle.

[0034] A first servo motor 71 for driving the bidirectional lead screw 72 to rotate is installed on one side of the top of the strip 1, and the driving end of the first servo motor 71 is connected to one end of the bidirectional lead screw 72. The middle part of the transmission bar 73 has a threaded hole that is threaded to the bidirectional lead screw 72. Two guide rails 74 are installed on the top of the strip 1, and sliders 75 that slide on the guide rails 74 are installed at both ends of the bottom of the transmission bar 73.

[0035] During use, the spacing between the two clamping strips 8 can be adjusted by the thread drive, which facilitates quick assembly, disassembly and replacement of the adhesive frame. At the same time, adjusting the spacing between the two clamping strips 8 also adapts to the clamping operation of adhesive frames of different sizes, improving its adaptability. Furthermore, fixing the adhesive frame, that is, fixing the adhesive-making glass sheet, eliminates the need for manual hand-held fixing, which improves convenience and also ensures the stability of the semi-circular rubber head 6 when striking the adhesive-making glass sheet.

[0036] The remaining structure is the same as that in Example 1.

[0037] Based on embodiments 1-2, the working principle of this utility model is as follows: When two adhesive glass sheets are combined, they are fixed on the adhesive clamping frame. Then, the two support ends at the bottom of the adhesive clamping frame are placed on the top of the two transmission bars 73 on both sides. The two clamping bars 8 are located between the two support ends at the bottom of the adhesive clamping frame. The first servo motor 71 drives the bidirectional screw 72 to rotate. Under the transverse thread transmission action of the bidirectional screw 72 and the threaded hole on the transmission bar 73, the two transmission bars 73 move to both sides respectively, that is, the distance between the two transmission bars 73 is increased. Through the contact between the clamping bars 8 and the support ends at the bottom of the adhesive clamping frame, the adhesive clamping frame is clamped and fixed by the two clamping bars 8. Conversely, the bidirectional screw 72 is driven to rotate in the opposite direction, that is, the fixation of the adhesive clamping frame is released.

[0038] The second servo motor 510 drives the rotating disk 52 to rotate, which in turn drives the mounting shaft 53 to rotate in a circular motion. Under the linkage of the mounting shaft 53, the push-pull bar 54 and the U-shaped frame 55, the push-pull bar 54 pushes and pulls the movable column 58 back and forth, so that the movable column 58 is in a lateral reciprocating movement state. That is, the semi-circular rubber head 6 is in a high-frequency reciprocating lateral translation state. In other words, the semi-circular rubber head 6 taps the glue-making glass slide on one side at a high frequency. The tapping vibration promotes the rapid and comprehensive diffusion of the glue liquid between the two glue-making glass slides.

[0039] It should be noted that the above embodiments are only used to illustrate the technical solution of this utility model and are not intended to limit it. Although this utility model has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solution of this utility model without departing from the spirit and scope of the technical solution of this utility model, and all such modifications or substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A tool for rapidly preparing polyacrylamide gel, comprising a strip (1), characterized in that: The top of the strip (1) is fixed with a clamping assembly (7), and the tops of the two movable ends of the clamping assembly (7) are both fixed with clamping strips (8). A U-frame plate (2) is integrally formed and connected to one side of the strip plate (1). A horizontal linear motor (3) is installed on the top of the U-frame plate (2). A vertical linear motor (4) is fixed to the translating movable end of the horizontal linear motor (3). A reciprocating drive structure (5) is installed on the lifting end of the vertical linear motor (4). A semi-circular rubber head (6) is fixed to the movable end of the reciprocating drive structure (5).

2. The tool for rapidly preparing polyacrylamide gel according to claim 1, characterized in that: The reciprocating drive structure (5) includes a lifting chamber (51) fixed on the lifting end of the longitudinal linear motor (4). A positioning sleeve (56) is welded to the side of the lifting chamber (51) near the strip (1). A movable column (58) is slidably installed inside the positioning sleeve (56), and one end of the movable column (58) is located outside the positioning sleeve (56) and is fixedly connected to the semi-circular rubber head (6). A drive body for driving the movable column (58) to move laterally is installed inside the lifting chamber (51).

3. The tool for rapidly preparing polyacrylamide gel according to claim 2, characterized in that: Both sides of the positioning sleeve (56) are fixed with slide chambers (57), and both sides of the periphery of the movable column (58) are equipped with guide strips (59) that slide inside the slide chambers (57).

4. The tool for rapidly preparing polyacrylamide gel according to claim 3, characterized in that: The driving body includes a second servo motor (510) fixed inside the lifting chamber (51) on the side away from the movable column (58), and a U-shaped frame (55) installed on the end of the movable column (58) away from the semi-circular rubber head (6). The driving end of the second servo motor (510) is fixedly connected to a rotating disk (52). An installation shaft (53) is rotatably installed on the outer edge of the end of the rotating disk (52) away from the second servo motor (510). A push-pull bar (54) is rotatably connected to the installation shaft (53), and the end of the push-pull bar (54) away from the installation shaft (53) is rotatably installed inside the U-shaped frame (55).

5. The tool for rapidly preparing polyacrylamide gel according to claim 4, characterized in that: The clamping assembly (7) includes a bidirectional lead screw (72) rotatably mounted on the top of the strip plate (1) via a bearing. Both sides of the bidirectional lead screw (72) are threaded with transmission bars (73), and the transmission bars (73) are laterally slidably mounted on the top of the strip plate (1). The clamping bar (8) is fixed on the top of the transmission bar (73) and on one side near the middle.

6. The tool for rapidly preparing polyacrylamide gel according to claim 5, characterized in that: A first servo motor (71) for driving the bidirectional lead screw (72) to rotate is installed on one side of the top of the strip (1). The middle part of the transmission bar (73) has a threaded hole that is threaded to the bidirectional lead screw (72). Two guide rails (74) are installed on the top of the strip (1). Slider (75) that slides on the guide rails (74) are installed at both ends of the bottom of the transmission bar (73).

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