A tissue pulverizing device for genetic testing

Abstract

The utility model relates to gene detection technical field especially is a kind of tissue pulverizing device for gene detection, including shell, the inner bottom of shell is fixedly connected with limit seat, the rotating cylinder is movably connected on the limit seat, the top of rotating cylinder is fixedly connected with gear ring, the top of shell is installed with upper cover, the top of upper cover is fixedly connected with motor, the output of motor is fixedly connected with rotating shaft, the bottom of rotating shaft is fixedly connected with cutting knife, the outer surface of rotating shaft is fixedly connected with first gear, the top of upper cover is movably connected with connecting shaft, the bottom of connecting shaft is fixedly connected with second gear, the left side of second gear is engagedly connected with the side of first gear, the right side of second gear is engagedly connected with the inner ring side of gear ring.The utility model provides a kind of tissue pulverizing device for gene detection, which can pulverize tissue and filter the pulverized tissue.

Description

Technical Field

[0001] This utility model relates to the field of gene detection, and in particular to a tissue pulverizer for gene detection. Background Technology

[0002] In the gene detection process, tissue fragmentation is a key step in sample pretreatment. Its core purpose is to break down the cellular structure of the tissue (such as cell walls and cell membranes) and release the genomic DNA (or RNA) inside the cells, providing qualified starting material for subsequent nucleic acid extraction, amplification, and detection (such as PCR and sequencing).

[0003] Tissues are composed of a large number of tightly connected cells. The cell wall (in plant / fungal tissues) and cell membrane can hinder the release of nucleic acids. Disruption can destroy these structures through physical or chemical means, exposing the DNA in the cell nucleus (or RNA in the cytoplasm) to the extraction buffer. After the buffer is extracted and tested, the genes can be detected. Utility Model Content

[0004] In view of this, the present invention provides a tissue pulverizing device for gene detection. The main technical problem to be solved is: to provide a tissue pulverizing device for gene detection that can pulverize tissue and filter the pulverized tissue.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a tissue pulverizing device for gene detection, comprising a shell, a limiting seat fixedly connected to the inner bottom of the shell, a rotating cylinder movably connected to the limiting seat, a toothed ring fixedly connected to the top of the rotating cylinder, a top cover installed on the top of the shell, a motor fixedly connected to the top of the top cover, a rotating shaft fixedly connected to the output end of the motor, a cutting blade fixedly connected to the bottom of the rotating shaft, the cutting blade being located inside the rotating cylinder, a first gear fixedly connected to the outer surface of the rotating shaft, a connecting shaft movably connected to the top of the top cover, a second gear fixedly connected to the bottom of the connecting shaft, the left side of the second gear meshing with the side of the first gear, and the right side of the second gear meshing with the inner ring side of the toothed ring.

[0006] By adopting the above technical solution, during use, the top cover is opened, the tissue to be pulverized is added into the rotating drum, the top cover is closed, the motor is started, the rotating shaft is driven to rotate, and in turn the cutting blade is driven to rotate, pulverizing and cutting the tissue in the rotating drum. At the same time, the first gear on the rotating shaft drives the second gear to rotate through meshing transmission. The second gear meshes with the gear ring at the top of the rotating drum, thereby driving the rotating drum to rotate in the opposite direction on the limit seat. This design of the cutting blade and the rotating drum rotating in opposite directions can generate stronger shearing force and friction, so that the tissue is more fully pulverized, effectively improving the pulverization efficiency.

[0007] As a further description of the above technical solution:

[0008] The outer surface of the rotating cylinder is provided with through holes, and there are multiple through holes.

[0009] By adopting the above technical solution, during the pulverization process, multiple through holes on the outer surface of the rotating cylinder allow larger tissue blocks to remain inside the rotating cylinder for further pulverization, thus achieving preliminary blocking and filtration of the pulverized tissue.

[0010] As a further description of the above technical solution:

[0011] The connecting shaft is mounted on the top of the cover via bearings, and the top of the connecting shaft extends above the cover.

[0012] By adopting the above technical solution, the top area of ​​the rotating connecting shaft can rotate the second gear and adjust its position so that the second gear can better mesh with the gear ring when the top cover is installed.

[0013] As a further description of the above technical solution:

[0014] A discharge pipe is fixedly connected to the outer surface of the outer casing, and the discharge pipe is located on the lower right side of the outer casing.

[0015] By adopting the above technical solution, after the pulverization is completed, the pulverized and filtered tissue can be discharged through the discharge tube on the lower right side of the outer shell for subsequent nucleic acid extraction and other operations.

[0016] As a further description of the above technical solution:

[0017] A water inlet pipe is fixedly connected to the outer surface of the outer casing, and the water inlet pipe is located on the upper left side of the outer casing.

[0018] By adopting the above technical solution, when liquid reagents (such as buffer solutions) need to be added during the pulverization process, they can be added through the water inlet pipe on the upper left side of the outer shell, which is convenient and quick.

[0019] As a further description of the above technical solution:

[0020] The outer surface of the top cover is fixedly connected with a latch, and there are multiple latches. The bottom of the latch is movably connected to the outer surface of the outer shell.

[0021] By adopting the above technical solution, the multiple locking designs on the outer surface of the top cover can ensure a tight connection between the top cover and the outer shell, preventing the top cover from loosening due to vibration or other reasons during the crushing process, thus ensuring the stability and safety of the device operation.

[0022] By employing the above technical solution, the tissue pulverizing device for gene detection of this utility model has at least the following beneficial effects:

[0023] 1. Compared with existing technologies, this tissue pulverizing device for gene detection uses a motor to drive a rotating shaft, which in turn drives a cutting blade to rotate, pulverizing and cutting the tissue inside the rotating cylinder. At the same time, the first gear on the rotating shaft meshes with a second gear, driving the second gear to rotate. The second gear then meshes with a gear ring at the top of the rotating cylinder, thereby driving the rotating cylinder to rotate in the opposite direction on the limiting seat. This design of the cutting blade and the rotating cylinder rotating in opposite directions can generate stronger shearing force and friction, making the tissue more thoroughly pulverized and effectively improving the pulverizing efficiency.

[0024] 2. Compared with the existing technology, this tissue pulverizing device for gene detection, during the pulverizing process, allows larger tissue blocks to remain inside the rotating cylinder through multiple through holes on the outer surface of the rotating cylinder for further pulverization, thus achieving preliminary blocking and filtration of the pulverized tissue. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall structure from a first-view perspective of the present invention.

[0026] Figure 2 This is a schematic diagram of the overall structure from a second perspective proposed in this utility model;

[0027] Figure 3 This is a first-view sectional view of the internal structure proposed in this utility model;

[0028] Figure 4 This is a second-view sectional view of the internal structure proposed in this utility model.

[0029] Legend:

[0030] 1. Outer shell; 2. Limiting seat; 3. Rotating cylinder; 4. Through hole; 5. Gear ring; 6. Top cover; 7. Motor; 8. Rotating shaft; 9. Cutting blade; 10. First gear; 11. Connecting shaft; 12. Second gear; 13. Discharge pipe; 14. Water inlet pipe; 15. Lock. Detailed Implementation

[0031] Reference Figure 1-4This utility model provides a tissue pulverizing device for gene detection: It includes a housing 1, with a limiting seat 2 fixedly connected to the inner bottom of the housing 1. The limiting seat 2 is installed on the inner bottom of the housing 1 by screws or other fasteners. A rotating cylinder 3 is movably connected to the limiting seat 2, and the rotating cylinder 3 can rotate on the limiting seat 2. A toothed ring 5 is fixedly connected to the top of the rotating cylinder 3. A top cover 6 is installed on the top of the housing 1, and a motor 7 is fixedly connected to the top of the top cover 6. A rotating shaft 8 is fixedly connected to the output end of the motor 7. A cutting blade 9 is fixedly connected to the bottom of the rotating shaft 8, and the cutting blade 9 can pulverize the tissue inside the rotating cylinder 3. The cutting blade 9 is located inside the rotating cylinder 3. A first gear 10 is fixedly connected to the outer surface of the rotating shaft 8. A connecting shaft 11 is movably connected to the top of the top cover 6, and a second gear 10 is fixedly connected to the bottom of the connecting shaft 11. 2. The left side of the second gear 12 meshes with the side of the first gear 10, and the right side of the second gear 12 meshes with the inner ring side of the gear ring 5. In use, the upper cover 6 is opened and the tissue to be pulverized is added into the rotating cylinder 3. Then the upper cover 6 is closed, the motor 7 is started, and the rotating shaft 8 is driven to rotate, which in turn drives the cutting blade 9 to rotate, pulverizing and cutting the tissue in the rotating cylinder 3. At the same time, the first gear 10 on the rotating shaft 8 drives the second gear 12 to rotate through meshing with the second gear 12. The second gear 12 meshes with the gear ring 5 at the top of the rotating cylinder 3, thereby driving the rotating cylinder 3 to rotate in the opposite direction on the limiting seat 2. This design of the cutting blade 9 and the rotating cylinder 3 rotating in the opposite direction can generate stronger shearing force and friction, so that the tissue is more fully pulverized, effectively improving the pulverization efficiency.

[0032] The outer surface of the rotating cylinder 3 is provided with through holes 4. There are multiple through holes 4, and the diameter of the through holes 4 can be 0.3-1mm. Large tissue pieces are blocked and filtered (for ease of illustration, the diameter of the through holes 4 is drawn to be larger in the attached figure). During the crushing process, the multiple through holes 4 on the outer surface of the rotating cylinder 3 keep the larger tissue pieces inside the rotating cylinder 3 for further crushing, thus achieving the initial blocking and filtering of the crushed tissue.

[0033] The connecting shaft 11 is mounted on the top of the upper cover 6 via a bearing. The top of the connecting shaft 11 extends above the upper cover 6. Rotating the top area of ​​the connecting shaft 11 can rotate the second gear 12 and adjust the position of the second gear 12 so that the second gear 12 can better mesh with the gear ring 5 when the upper cover 6 is installed.

[0034] An outlet tube 13 is fixedly connected to the outer surface of the outer shell 1. The outlet tube 13 is located on the lower right side of the outer shell 1. After the pulverization is completed, the pulverized and filtered tissue can be discharged through the outlet tube 13 on the lower right side of the outer shell 1 for subsequent nucleic acid extraction and other operations. An inlet tube 14 is fixedly connected to the outer surface of the outer shell 1. The inlet tube 14 is located on the upper left side of the outer shell 1. If liquid reagents such as buffer solution need to be added during the pulverization process, they can be added through the inlet tube 14 on the upper left side of the outer shell 1, which is convenient and quick.

[0035] The outer surface of the top cover 6 is fixedly connected with a latch 15. There are multiple latches 15. The bottom of the latch 15 is movably connected to the outer surface of the outer shell 1. The latch 15 mainly serves to connect the top cover 6 and the outer shell 1. Depending on different needs, the latch 15 can be replaced with screws or other connecting parts. The design of multiple latches 15 on the outer surface of the top cover 6 can ensure that the top cover 6 is tightly connected to the outer shell 1, preventing the top cover 6 from loosening due to vibration or other reasons during the crushing process, thus ensuring the stability and safety of the device operation.

[0036] Working principle: During use, the motor 7 drives the rotating shaft 8 to rotate, which in turn drives the cutting blade 9 to rotate, pulverizing and cutting the tissue inside the rotating cylinder 3. At the same time, the first gear 10 on the rotating shaft 8 meshes with the second gear 12, driving the second gear 12 to rotate. The second gear 12 then meshes with the toothed ring 5 at the top of the rotating cylinder 3, thereby driving the rotating cylinder 3 to rotate in the opposite direction on the limiting seat 2. This design of the cutting blade 9 and the rotating cylinder 3 rotating in the opposite direction can generate stronger shearing force and friction, so that the tissue is more thoroughly pulverized, effectively improving the pulverization efficiency. In addition, during the pulverization process, the multiple through holes 4 on the outer surface of the rotating cylinder 3 keep larger tissue blocks inside the rotating cylinder 3 for further pulverization, achieving preliminary interception and filtration of the pulverized tissue.

[0037] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A tissue pulverizer for gene detection, comprising a housing (1), characterized in that: The inner bottom of the outer shell (1) is fixedly connected to a limiting seat (2), and a rotating cylinder (3) is movably connected to the limiting seat (2). A gear ring (5) is fixedly connected to the top of the rotating cylinder (3). A top cover (6) is installed on the top of the outer shell (1). A motor (7) is fixedly connected to the top of the top cover (6). A rotating shaft (8) is fixedly connected to the output end of the motor (7). A cutting blade (9) is fixedly connected to the bottom of the rotating shaft (8). The cutting blade (9) is located inside the rotating cylinder (3). A first gear (10) is fixedly connected to the outer surface of the rotating shaft (8). A connecting shaft (11) is movably connected to the top of the top cover (6). A second gear (12) is fixedly connected to the bottom of the connecting shaft (11). The left side of the second gear (12) meshes with the side of the first gear (10), and the right side of the second gear (12) meshes with the inner ring side of the gear ring (5).

2. The tissue pulverizer for gene detection according to claim 1, characterized in that: The outer surface of the rotating cylinder (3) is provided with through holes (4), and there are multiple through holes (4).

3. The tissue pulverizer for gene detection according to claim 1, characterized in that: The connecting shaft (11) is mounted on the top of the cover (6) by bearings, and the top of the connecting shaft (11) extends above the cover (6).

4. The tissue pulverizer for gene detection according to claim 1, characterized in that: A discharge pipe (13) is fixedly connected to the outer surface of the outer shell (1), and the discharge pipe (13) is located on the lower right side of the outer shell (1).

5. The tissue pulverizer for gene detection according to claim 1, characterized in that: A water inlet pipe (14) is fixedly connected to the outer surface of the outer shell (1), and the water inlet pipe (14) is located on the upper left side of the outer shell (1).

6. The tissue pulverizer for gene detection according to claim 1, characterized in that: The outer surface of the top cover (6) is fixedly connected with a buckle (15), and there are multiple buckles (15). The bottom of the buckle (15) is movably connected to the outer surface of the outer shell (1).

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