A neural stem cell exosome extraction centrifuge device
Patent Information
- Application Number
- CN202521653069.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-08-05
- Publication Date
- 2026-09-04
- Estimated Expiration
- 2035-08-05
AI Technical Summary
[0004]为了弥补以上不足，本实用新型提供了一种神经干细胞外泌体提取离心装置，旨在解决现有装置中在提取外泌体时，需要操作人员多次离心和多次分离，使得过程繁琐，耗时费力，效率低下的问题
[0015]1. In this utility model, the centrifugal rotor is driven by the main body of the device to achieve the function of low-speed, medium-speed and high-speed centrifugation of the container assembly. The preparation liquid is provided to the container assembly through the liquid preparation container group. Through the cooperation of connecting pipe one, connecting pipe two, peristaltic pump one, peristaltic pump two, metering component, pressure sensor one, filter screen assembly and fastening cap, the problem of the existing device requiring multiple centrifugation and separation by the operator when extracting exosomes is solved, which makes the process cumbersome, time-consuming and labor-intensive and inefficient.
Smart Images
Figure CN224712221U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of exosome extraction technology, and in particular to a centrifugation device for extracting exosomes from neural stem cells. Background Technology
[0002] Neural stem cell exosomes are a type of extracellular vesicle with an intact membrane structure secreted by neural stem cells. They contain various bioactive factors such as lipids, proteins, and RNA, and have a diameter of approximately 30–150 nm. Neural stem cell-derived exosomes have significant research value and application prospects in areas such as neural repair and disease treatment.
[0003] Currently, exosomes from neural stem cells are mainly extracted using centrifugation. Existing devices require operators to perform multiple centrifugations and separations during exosome extraction, making the process cumbersome, time-consuming, labor-intensive, and inefficient. Utility Model Content
[0004] To overcome the above shortcomings, this utility model provides a centrifugation device for extracting neural stem cell exosomes, which aims to solve the problem that existing devices require operators to perform multiple centrifugations and separations during exosome extraction, making the process cumbersome, time-consuming, labor-intensive, and inefficient.
[0005] To achieve the above objectives, this utility model provides the following technical solution: a centrifuge device for extracting neural stem cell exosomes, comprising a device body, a centrifuge rotor rotatably connected to the inner bottom wall of the device body, a container assembly and a liquid preparation container assembly disposed on the centrifuge rotor, a fastening cap disposed on the upper side of the centrifuge rotor, a peristaltic pump II disposed on the lower side of the fastening cap, multiple connecting tubes I fixedly disposed at the output end of the peristaltic pump II, the two ends of the connecting tubes I respectively disposed inside the container assembly and the liquid preparation container assembly, a peristaltic pump I disposed on the lower side of the peristaltic pump II, multiple connecting tubes II disposed inside the container assembly, the output end of the peristaltic pump I fixedly disposed on the outer wall of the multiple connecting tubes II, a metering component disposed inside the connecting tubes I, and a pressure sensor I and a filter assembly disposed inside the connecting tubes II.
[0006] The above technical solution solves the problem that existing devices require multiple centrifugations and separations during exosome extraction, which is cumbersome, time-consuming, labor-intensive, and inefficient.
[0007] Preferably, the container assembly includes a first container, a second container, a third container, and a fourth container. The first container, the second container, the third container, and the fourth container are connected sequentially by three connecting pipes. The first container, the second container, the third container, and the fourth container are all disposed on the upper side of the centrifugal rotor. The outer walls of both ends of the connecting pipe connecting the first container and the second container are respectively fixedly disposed at one end of two connecting pipes. The outer wall of the end where the connecting pipe connects to the third container is fixedly disposed at one end of a connecting pipe. The inner wall of the other end of the connecting pipe is provided with a one-way valve.
[0008] Preferably, the liquid preparation container group includes liquid preparation bottle one, liquid preparation bottle two, and liquid preparation bottle three. Liquid preparation bottle one, liquid preparation bottle two, and liquid preparation bottle three are all located on the upper side of the centrifugal rotor, and one end of each of the three connecting pipes one is located inside liquid preparation bottle one, liquid preparation bottle two, and liquid preparation bottle three, respectively.
[0009] Preferably, the filter assembly includes filter one, filter two, and filter three. Filter one is disposed on the inner wall of connecting pipe two inside the first container, filter two is disposed on the inner wall of connecting pipe two inside the second container, and filter three is disposed on the inner wall of connecting pipe two inside the third container.
[0010] Preferably, a gear is rotatably connected to the inner bottom wall of the device body, the outer wall of the gear is slidably connected to the lower side of the centrifugal rotor, a cylinder is provided inside the device body, the output end of the cylinder is fixedly provided to the lower side of the centrifugal rotor, a fixing screw is fixedly connected to the upper middle part of the centrifugal rotor, a nut is provided on the upper side of the fastening cover, and the inner wall of the nut is threadedly connected to the upper outer wall of the fixing screw.
[0011] Preferably, an upper cover is rotatably connected to the upper side of the fixing screw, the lower side of the upper cover is attached to the upper side of the device body, and a control terminal is provided on the upper side of the device body. The control terminal is electrically connected to the device body, cylinder, metering component, pressure sensor one, peristaltic pump one, and peristaltic pump two.
[0012] Preferably, the metering component includes a flow sensor, which is disposed inside the connecting pipe and is electrically connected to the control terminal.
[0013] Preferably, the metering component includes a second pressure sensor, which is disposed inside the first connecting pipe and is electrically connected to the control terminal.
[0014] This utility model has the following beneficial effects:
[0015] 1. In this utility model, the centrifugal rotor is driven by the main body of the device to achieve the function of low-speed, medium-speed and high-speed centrifugation of the container assembly. The preparation liquid is provided to the container assembly through the liquid preparation container group. Through the cooperation of connecting pipe one, connecting pipe two, peristaltic pump one, peristaltic pump two, metering component, pressure sensor one, filter screen assembly and fastening cap, the problem of the existing device requiring multiple centrifugation and separation by the operator when extracting exosomes is solved, which makes the process cumbersome, time-consuming and labor-intensive and inefficient.
[0016] 2. In this utility model, through the driving of peristaltic pump one and peristaltic pump two, through the setting of first container, second container, third container and fourth container, through the setting of liquid preparation bottle one, liquid preparation bottle two and liquid preparation bottle three, and through the cooperation of connecting pipe one, connecting pipe two, pressure sensor one and filter screen assembly, the automatic multi-stage filtration and purification function of the device is realized.
[0017] 3. In this utility model, by designing the metering component as a flow sensor or pressure sensor II, the volume or pressure data of the preparation liquid passing through the connecting pipe I can be measured in real time, and the device can automatically add the preparation liquid through the control of the control terminal. Attached Figure Description
[0018] Figure 1 This is a three-dimensional structural diagram of a centrifugation device for extracting neural stem cell exosomes according to the present invention;
[0019] Figure 2 This is a schematic diagram of the exploded structure of the centrifuge rotor of a centrifuge device for extracting neural stem cell exosomes according to this utility model;
[0020] Figure 3 This is a schematic diagram of the cylinder structure of a centrifuge device for extracting neural stem cell exosomes according to this utility model;
[0021] Figure 4 This is a partial structural diagram of the metering component of a centrifuge device for extracting neural stem cell exosomes according to the present invention.
[0022] Figure 5 This is a schematic diagram of the two pressure sensors in a centrifugation device for extracting neural stem cell exosomes according to the present invention.
[0023] Figure 6 This is a partial structural diagram of the filter assembly of a centrifuge device for extracting exosomes from neural stem cells, as proposed in this utility model.
[0024] Legend:
[0025] 1. Top cover; 2. Control terminal; 3. Device body; 4. Nut; 5. Fastening cover; 6. Container assembly; 60. First container; 61. Second container; 62. Third container; 63. Fourth container; 7. Centrifugal rotor; 8. Gear; 9. Cylinder; 10. Fixing screw; 11. Liquid preparation container group; 110. Liquid preparation bottle one; 111. Liquid preparation bottle two; 112. Liquid preparation bottle three; 12. Metering assembly; 13. Flow sensor; 14. Connecting pipe one; 15. Connecting pipe two; 16. Pressure sensor one; 17. Peristaltic pump one; 18. Peristaltic pump two; 19. Pressure sensor two; 20. Filter assembly. Detailed Implementation
[0026] The technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of this utility model.
[0027] Example 1:
[0028] Reference Figure 2 , Figure 4 and Figure 6 This utility model provides an embodiment of a neural stem cell exosome extraction centrifugation device, comprising a device body 3, a centrifugal rotor 7 rotatably connected to the inner bottom wall of the device body 3, a container assembly 6 and a liquid preparation container group 11 disposed on the centrifugal rotor 7, a fastening cover 5 disposed on the upper side of the centrifugal rotor 7, a peristaltic pump 18 disposed on the lower side of the fastening cover 5, multiple connecting pipes 14 fixedly disposed at the output end of the peristaltic pump 18, the two ends of the connecting pipes 14 being respectively disposed inside the container assembly 6 and the liquid preparation container group 11, a peristaltic pump 17 disposed on the lower side of the peristaltic pump 18, multiple connecting pipes 15 disposed inside the container assembly 6, the output end of the peristaltic pump 17 being fixedly disposed on the outer wall of the multiple connecting pipes 15, a metering component 12 disposed inside the connecting pipes 14, and a pressure sensor 16 and a filter assembly 20 disposed inside the connecting pipes 15.
[0029] Specifically, the main body 3 is a centrifuge that does not require balancing. It can use the Zhongke Zhongjia KDC-220HR high-speed refrigerated centrifuge or the Zhongke Zhongjia HC-3018R high-speed refrigerated centrifuge, and it has low-speed, medium-speed and high-speed functions at the same time. This is existing technology. Peristaltic pump 17 and peristaltic pump 28 are both multi-channel peristaltic pumps that can switch between different channels. They can use Kamer micro pumps, which is existing technology.
[0030] When using this device, place the neural stem cell exosome mixture and the preparation liquid into container assembly 6 and preparation container group 11 respectively, then place container assembly 6 and preparation container group 11 into centrifuge rotor 7, cover with fastening cap 5, so that connecting tube one 14 and connecting tube two 15 are inserted into the corresponding container assembly 6 or preparation container group 11, and start centrifugation.
[0031] During the process of switching between low-speed, medium-speed, and high-speed centrifugation rotation in the main body 3, based on the amount of original liquid in container assembly 6 and combined with historical centrifugation results data, the peristaltic pump 18 drives the connecting tube 14, causing the connecting tube 14 to extract a certain range of prepared liquid from the liquid preparation container group 11 into container assembly 6 at different stages of low-speed, medium-speed, and high-speed centrifugation through the metering component 12. Then, the peristaltic pump 17 drives the connecting tube 15, and the filter assembly 20 filters the liquid, realizing the filtration and extraction of neural stem cell exosomes after centrifugation at different speed stages. This solves the problem in existing devices that require multiple centrifugations and separations by operators when extracting exosomes, making the process cumbersome, time-consuming, labor-intensive, and inefficient.
[0032] Reference Figure 2 and Figure 4 The container assembly 6 includes a first container 60, a second container 61, a third container 62, and a fourth container 63. The first container 60, the second container 61, the third container 62, and the fourth container 63 are connected sequentially by three connecting pipes 15. The first container 60, the second container 61, the third container 62, and the fourth container 63 are all located on the upper side of the centrifugal rotor 7. The outer walls of both ends of the connecting pipe 15 connecting the first container 60 and the second container 61 are respectively fixed to one end of two connecting pipes 14. The outer wall of the end of the connecting pipe 15 connecting the third container 62 is fixed to one end of a connecting pipe 14. The inner wall of the other end of the connecting pipe 14 is provided with a one-way valve.
[0033] Specifically, the first container 60, the second container 61, the third container 62, and the fourth container 63 are containers with stoppers. When in use, the neural stem cell exosome mixture is poured into the first container 60. The first container 60 and the second container 61 are connected by the first connecting tube 2 15, the second container 61 and the third container 62 are connected by the second connecting tube 2 15, and the third container 62 and the fourth container 63 are connected by the third connecting tube 2 15.
[0034] The peristaltic pump 18 drives the connecting tube 14, thereby transporting the prepared solution in the liquid preparation container group 11 to the first container 60. At this time, the prepared solution plays a dilution role, and cells and large fragments are removed by low-speed centrifugation of the device body 3. Then, the peristaltic pump 17 drives the first connecting tube 15, and the supernatant in the first container 60 is extracted into the second container 61 through the filter assembly 20 in the connecting tube 15. The pressure sensor 16 on the first connecting tube 15 monitors the changes in liquid pressure inside the connecting tube 15 in real time to determine whether the extraction is complete.
[0035] Next, the peristaltic pump 18 drives the connecting tube 14 to deliver the prepared solution in the preparation container group 11 to the second container 61. At this time, the prepared solution inhibits protein degradation and maintains pH stability. Driven by the device body 3, the mixture in the second container 61 is centrifuged at medium speed to remove subcellular structures. Then, the peristaltic pump 17 drives the second connecting tube 15, and through the cooperation of the pressure sensor 16 and the filter assembly 20 in the second connecting tube 15, the supernatant in the second container 61 is drawn into the third container 62.
[0036] Next, the peristaltic pump 18 drives the connecting tube 14, transporting the prepared solution in the preparation container group 11 to the second container 61. At this time, the prepared solution serves to pre-cool and purify the solution. Then, the peristaltic pump 17 drives the third connecting tube 15, and through the cooperation of the pressure sensor 16 and the filter assembly 20 in the third connecting tube 15, the supernatant in the third container 62 is drawn into the fourth container 63. At this time, the remaining contents in the third container 62 are the neural stem cell exosomes after centrifugation, filtration and purification. During this process, the design of the one-way valve inside the connecting tube 14 prevents the supernatant from flowing into the connecting tube 14, thereby realizing the fully automatic multi-stage centrifugation, filtration and purification function of neural stem cell exosomes.
[0037] Reference Figure 2 and Figure 4 The liquid preparation container group 11 includes liquid preparation bottle one 110, liquid preparation bottle two 111 and liquid preparation bottle three 112. Liquid preparation bottle one 110, liquid preparation bottle two 111 and liquid preparation bottle three 112 are all located on the upper side of the centrifugal rotor 7. One end of the three connecting pipes one 14 is respectively located inside the liquid preparation bottle one 110, liquid preparation bottle two 111 and liquid preparation bottle three 112.
[0038] Specifically, preparation bottles 110, 111, and 112 are all stoppered preparation bottles. Preparation bottle 110 contains phosphate buffer, preparation bottle 211 contains phosphate buffer and protease inhibitor, and preparation bottle 112 contains pre-cooled phosphate buffer. Before low-speed centrifugation, peristaltic pump 218 drives connecting tube 14 in preparation bottle 110 to draw the phosphate buffer in preparation bottle 110 into the first container 60. Before medium-speed centrifugation, peristaltic pump 218 drives connecting tube 14 in preparation bottle 211 to draw the phosphate buffer and protease inhibitor in preparation bottle 211 into the second container 61. Before high-speed centrifugation, peristaltic pump 218 drives connecting tube 14 in preparation bottle 112 to draw the pre-cooled phosphate buffer in preparation bottle 112 into the third container 62. This, combined with centrifugation, helps to achieve the fully automated centrifugation function of the device.
[0039] Reference Figure 4 and Figure 6 The filter assembly 20 includes filter one, filter two and filter three. Filter one is disposed on the inner wall of the connecting pipe two 15 inside the first container 60, filter two is disposed on the inner wall of the connecting pipe two 15 inside the second container 61, and filter three is disposed on the inner wall of the connecting pipe two 15 inside the third container 62.
[0040] Specifically, filter one is used to filter cells and large debris, with a pore size range of 5~10μm; filter two is used to filter subcellular structures, with a pore size range of 0.22~1μm; and filter three is used to filter exosomes, with a pore size range of 0.1~0.2μm. By using filters with different pore sizes, the device achieves multi-stage filtration.
[0041] Reference Figure 2 and Figure 3 The inner bottom wall of the device body 3 is rotatably connected to a gear 8, and the outer wall of the gear 8 is slidably connected to the lower side of the centrifugal rotor 7. A cylinder 9 is installed inside the device body 3, and the output end of the cylinder 9 is fixedly installed on the lower side of the centrifugal rotor 7. A fixing screw 10 is fixedly connected to the middle of the upper side of the centrifugal rotor 7. A nut 4 is installed on the upper side of the fastening cover 5, and the inner wall of the nut 4 is threadedly connected to the upper outer wall of the fixing screw 10.
[0042] Specifically, the lower side of the centrifugal rotor 7 has a groove that matches the gear 8. The height of the groove is greater than the height of the gear 8, so that the centrifugal rotor 7 can slide and connect with the gear 8 to a certain extent. After different centrifugation stages are completed, the centrifugal rotor 7 is driven upward by the output end of the cylinder 9, so that the lower end of the connecting pipe 15 continuously penetrates into the container bottle to extract the supernatant, thereby helping to achieve the function of multi-stage filtration. The design of the fixing screw 10 can play a certain guiding and positioning role when installing the fastening cover 5, and the fastening cover 5 is fixed by the threaded connection between the fixing screw 10 and the nut 4.
[0043] Reference Figure 2 , Figure 4 and Figure 5 The upper side of the fixed screw 10 is rotatably connected to the upper cover 1. The lower side of the upper cover 1 is attached to the upper side of the device body 3. The upper side of the device body 3 is provided with a control terminal 2. The control terminal 2 is electrically connected to the device body 3, cylinder 9, metering component 12, pressure sensor 16, peristaltic pump 17, and peristaltic pump 18.
[0044] Specifically, the design of the top cover 1 provides a certain degree of safety protection during centrifugation. The control terminal 2 includes a controller and a touch screen. The controller can use a microprocessor, single-chip microcomputer, or PLC programmable controller. Through the control terminal 2, the operating status and parameters of the device body 3 can be viewed, adjusted, and controlled. The real-time measured data is transmitted to the control terminal 2 for processing and analysis through the metering component 12 and pressure sensor 16. The control terminal 2 also controls the drive of peristaltic pump 17 and peristaltic pump 18, thereby realizing the fully automatic and intelligent function of the device.
[0045] Reference Figure 1 and Figure 4 The metering component 12 includes a flow sensor 13, which is disposed inside the connecting pipe 14 and is electrically connected to the control terminal 2.
[0046] Specifically, the flow sensor 13 measures the volume of the liquid being prepared in the connecting pipe 14 in real time and transmits the data to the control terminal 2 for processing and analysis. This allows the control terminal 2 to control the opening and closing of the peristaltic pump 18, keeping the extracted liquid within a certain range, thus realizing the metering function of the metering component 12.
[0047] Example 2:
[0048] Reference Figure 1 and Figure 5The present invention also provides an embodiment in which the metering component 12 includes a second pressure sensor 19, which is disposed inside the first connecting pipe 14 and is electrically connected to the control terminal 2.
[0049] Specifically, the pressure sensor 19 measures the pressure of the liquid passing through the connecting pipe 14 in real time and transmits the data to the control terminal 2 for processing and analysis. By combining the pressure data with the analysis of time, the volume of liquid passing through within a certain time is determined, thereby enabling the control terminal 2 to control the start and stop of the peristaltic pump 18 to control the extracted liquid within a certain range, thus realizing the metering function of the metering component 12.
[0050] Working principle: When using this device, the neural stem cell exosome mixture is poured into the first container 60. The peristaltic pump 18 drives the connecting tube 14, thereby transporting the prepared solution in the preparation bottle 110 to the first container 60. If the metering component 12 is a flow sensor 13, the flow sensor 13 measures the volume of the prepared solution passing through the connecting tube 14 in real time. If the metering component 12 is a pressure sensor 19, the pressure sensor 19 measures the pressure of the prepared solution passing through the connecting tube 14 in real time. This allows the control terminal 2 to control the opening and closing of the peristaltic pump 18, keeping the extracted prepared solution within a certain range. Through low-speed centrifugation of the device body 3, the peristaltic pump 17 drives the first connecting tube 15. Through filtration by the filter assembly 20 in the connecting tube 15 and the cooperation of the pressure sensor 16, the supernatant in the first container 60 is extracted into the second container 61, thereby achieving the removal of cells and large debris.
[0051] Next, the peristaltic pump 18 drives the connecting tube 14 to deliver the prepared solution in the mixing bottle 111 to the second container 61. Driven by the device body 3, the mixture in the second container 61 is centrifuged at medium speed. Then, the peristaltic pump 17 drives the second connecting tube 15, and through the cooperation of the pressure sensor 16 and the filter assembly 20 in the second connecting tube 15, the supernatant in the second container 61 is extracted into the third container 62, thereby achieving the removal of subcellular structures.
[0052] Next, the peristaltic pump 18 drives the connecting tube 14 to deliver the prepared solution in the preparation bottle 112 to the third container 62. At this time, the prepared solution plays a role in pre-cooling and purification. Through the cooperation of the pressure sensor 16 and the filter assembly 20 in the third connecting tube 15, the supernatant in the third container 62 is drawn into the fourth container 63. At this time, the remaining substance in the third container 62 is the neural stem cell exosomes after centrifugation, filtration and purification. This solves the problem that in the existing device, when extracting exosomes, the operator needs to centrifuge and separate multiple times, which makes the process cumbersome, time-consuming and labor-intensive and inefficient.
[0053] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.
Claims
1. A centrifuge device for extracting neural stem cell exosomes, comprising a device body (3), characterized in that: A centrifugal rotor (7) is rotatably connected to the inner bottom wall of the device body (3). A container assembly (6) and a liquid preparation container group (11) are provided on the centrifugal rotor (7). A fastening cover (5) is provided on the upper side of the centrifugal rotor (7). A peristaltic pump (18) is provided on the lower side of the fastening cover (5). Multiple connecting pipes (14) are fixedly provided at the output end of the peristaltic pump (18). The two ends of the connecting pipes (14) are respectively provided inside the container assembly (6) and the liquid preparation container group (11). A peristaltic pump (17) is provided on the lower side of the peristaltic pump (18). Multiple connecting pipes (15) are provided inside the container assembly (6). The output end of the peristaltic pump (17) is fixedly provided on the outer wall of the multiple connecting pipes (15). A metering component (12) is provided inside the connecting pipe (14). A pressure sensor (16) and a filter assembly (20) are provided inside the connecting pipe (15).
2. The centrifuge device for extracting neural stem cell exosomes according to claim 1, characterized in that: The container assembly (6) includes a first container (60), a second container (61), a third container (62), and a fourth container (63). The first container (60), the second container (61), the third container (62), and the fourth container (63) are connected in sequence by three connecting pipes (15). The first container (60), the second container (61), the third container (62), and the fourth container (63) are all located on the upper side of the centrifugal rotor (7). The outer walls of the two ends of the connecting pipe (15) connecting the first container (60) and the second container (61) are respectively fixedly installed at one end of two connecting pipes (14). The outer wall of the end where the connecting pipe (15) connects to the third container (62) is fixedly installed at one end of a connecting pipe (14). The inner wall of the other end of the connecting pipe (14) is provided with a one-way valve.
3. The centrifugation device for extracting neural stem cell exosomes according to claim 1, characterized in that: The liquid preparation container group (11) includes liquid preparation bottle one (110), liquid preparation bottle two (111) and liquid preparation bottle three (112). Liquid preparation bottle one (110), liquid preparation bottle two (111) and liquid preparation bottle three (112) are all located on the upper side of the centrifugal rotor (7). One end of the three connecting pipes one (14) is respectively located inside liquid preparation bottle one (110), liquid preparation bottle two (111) and liquid preparation bottle three (112).
4. The neural stem cell exosome extraction centrifugation device according to claim 2, characterized in that: The filter assembly (20) includes filter one, filter two and filter three. Filter one is disposed on the inner wall of connecting pipe two (15) inside the first container (60). Filter two is disposed on the inner wall of connecting pipe two (15) inside the second container (61). Filter three is disposed on the inner wall of connecting pipe two (15) inside the third container (62).
5. The centrifuge device for extracting neural stem cell exosomes according to claim 1, characterized in that: The inner bottom wall of the device body (3) is rotatably connected to a gear (8), the outer wall of the gear (8) is slidably connected to the lower side of the centrifugal rotor (7), the inside of the device body (3) is provided with a cylinder (9), the output end of the cylinder (9) is fixedly provided on the lower side of the centrifugal rotor (7), a fixing screw (10) is fixedly connected to the middle of the upper side of the centrifugal rotor (7), a nut (4) is provided on the upper side of the fastening cover (5), and the inner wall of the nut (4) is threadedly connected to the upper outer wall of the fixing screw (10).
6. The centrifuge apparatus for extracting neural stem cell exosomes according to claim 5, characterized in that: The upper side of the fixed screw (10) is rotatably connected to the upper cover (1). The lower side of the upper cover (1) is attached to the upper side of the device body (3). The upper side of the device body (3) is provided with a control terminal (2). The control terminal (2) is electrically connected to the device body (3), cylinder (9), metering component (12), pressure sensor one (16), peristaltic pump one (17), and peristaltic pump two (18).
7. The neural stem cell exosome extraction centrifugation device according to claim 1, characterized in that: The metering component (12) includes a flow sensor (13), which is disposed inside the connecting pipe (14) and is electrically connected to the control terminal (2).
8. The neural stem cell exosome extraction centrifugation device according to claim 1, characterized in that: The metering component (12) includes a second pressure sensor (19), which is located inside the first connecting pipe (14) and is electrically connected to the control terminal (2).