A liquid leakage-proof nucleic acid extraction kit

Abstract

The utility model relates to the technical field of kit, concretely is a liquid leakage prevention nucleic acid extraction kit, including the box body, the upper surface symmetry of box body is equipped with nine reagent grooves, and the front surface of box body is equipped with the placing groove, the inner wall bottom wall of reagent groove is equipped with the through -hole, the through -hole with placing groove intercommunication, the inside slide coupling of placing groove has the placing plate, the upper surface symmetry of placing plate is equipped with nine recesss, the inside of recess is equipped with the sponge block, the lower surface symmetry of placing plate is equipped with three sliding slots, the sponge block with sliding slot intercommunication, the utility model discloses kit through " through -hole + placing plate + sponge block " forms complete liquid leakage diversion and adsorption system, and the liquid leakage can be accurately guided to the sponge block in the placing groove through the through -hole, avoids the diffusion of liquid leakage in the box body, effectively prevents the liquid leakage from polluting the reagent in the adjacent reagent groove, and reduces the reagent scrappage rate.

Description

Technical Field

[0001] This utility model relates to the field of reagent kit technology, specifically a leak-proof nucleic acid extraction reagent kit. Background Technology

[0002] Nucleic acid extraction is a core pre-processing step in molecular biology experiments (such as PCR detection and gene sequencing). As a key tool for carrying extraction reagents (such as lysis buffer, washing buffer, and elution buffer), the sealing, stability, and leakage warning capabilities of nucleic acid extraction kits directly affect the effectiveness of the reagents and the accuracy of experimental results.

[0003] Traditional nucleic acid extraction kits suffer from several technical drawbacks: First, most kits rely solely on simple reagent tanks to hold reagent containers, lacking a specific fixing structure. During transport and laboratory handling, these containers are prone to tilting and tipping, leading to reagent leakage. Second, there is no effective flow guidance or adsorption design after leakage, allowing the leaked reagent to spread along the kit body, contaminating reagents in adjacent tanks and rendering the entire kit unusable. Third, there is no leakage monitoring or early warning mechanism, requiring manual observation to detect leaks. If leakage is not detected in its early stages, it can lead to abnormal reagent concentrations in subsequent extraction steps, ultimately affecting nucleic acid extraction efficiency and purity. Therefore, a leak-proof nucleic acid extraction kit is proposed. Utility Model Content

[0004] In view of this, the present invention provides a leak-proof nucleic acid extraction kit to solve or alleviate the technical problems existing in the prior art, and at least provides a beneficial alternative.

[0005] The technical solution of this utility model is implemented as follows: A leak-proof nucleic acid extraction kit includes a box body. Nine reagent slots are symmetrically arranged on the upper surface of the box body, and a placement slot is arranged on the front surface of the box body. A through hole is formed on the bottom inner wall of the reagent slot, and the through hole communicates with the placement slot. A placement plate is slidably connected inside the placement slot. Nine grooves are symmetrically arranged on the upper surface of the placement plate, and a sponge block is placed inside the groove. Three sliding grooves are symmetrically arranged on the lower surface of the placement plate, and the sponge block communicates with the sliding groove. Nine humidity sensors are symmetrically installed on the bottom inner wall of the placement slot. Nine indicator lights are installed on the front surface of the box body.

[0006] More preferably, the box body has a battery compartment inside, the battery compartment is equipped with a storage battery, and a charging port is installed on one side of the box body, the charging port being electrically connected to the storage battery.

[0007] More preferably, the top of the box body is provided with a cover plate, and the two sides of the cover plate are fixedly connected with first buckle females, and the two sides of the box body are fixedly connected with first buckle females, and the first buckle females are engaged with the first buckle females.

[0008] More preferably, nine springs are symmetrically fixedly connected to the bottom of the cover plate, and a pressure plate is fixedly connected to the bottom of each spring.

[0009] More preferably, two second female snap fasteners are symmetrically installed on the front surface of the placement plate, and two second male snap fasteners are symmetrically fixedly connected to the front surface of the box body, with the second female snap fasteners engaging with the second male snap fasteners.

[0010] The present invention has the following advantages due to the adoption of the above technical solution:

[0011] I. The reagent kit of this utility model forms a complete leakage guidance and adsorption system through "through holes + placement plate + sponge block". The leakage can be accurately guided to the sponge block in the placement tank through the through holes, avoiding the leakage from spreading in the box and effectively preventing leakage from contaminating the reagents in adjacent reagent tanks, thus reducing the reagent scrap rate.

[0012] Second, the humidity sensor inside the placement tank and the indicator light on the front surface of the box form a linkage early warning mechanism. After leakage occurs, the indicator light will illuminate to intuitively indicate the location of the leakage, which solves the problem of "difficult to detect leakage" in traditional reagent kits. Operators can detect and deal with leakage in time, avoid abnormal reagent concentration caused by leakage, and ensure the accuracy and reliability of nucleic acid extraction experimental results.

[0013] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present invention will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description

[0014] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0015] Figure 1 This is a structural diagram of the present invention;

[0016] Figure 2 This is another structural diagram of the present invention;

[0017] Figure 3 This utility model Figure 2 Another perspective on the structure diagram;

[0018] Figure 4This is a cross-sectional structural diagram of the box body of this utility model.

[0019] Reference numerals: 1. Box body; 2. Reagent tank; 3. Through hole; 4. Placement slot; 5. Battery compartment; 6. Battery; 7. Cover plate; 8. Spring; 9. Pressure plate; 10. First female snap-fit; 11. First male snap-fit; 12. Charging port; 13. Placement plate; 14. Groove; 15. Sponge block; 16. Slide groove; 17. Second female snap-fit; 18. Second male snap-fit; 19. Humidity sensor; 20. Indicator light. Detailed Implementation

[0020] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this invention. Therefore, the drawings and description are considered exemplary in nature and not restrictive.

[0021] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0022] like Figure 1-4 As shown, this embodiment of the present invention provides a leak-proof nucleic acid extraction kit, including a box body 1. Nine reagent slots 2 are symmetrically arranged on the upper surface of the box body 1. A placement slot 4 is arranged on the front surface of the box body 1. A through hole 3 is arranged on the bottom wall of the inner wall of the reagent slot 2, and the through hole 3 communicates with the placement slot 4. A placement plate 13 is slidably connected inside the placement slot 4. Nine grooves 14 are symmetrically arranged on the upper surface of the placement plate 13. A sponge block 15 is arranged inside the groove 14. Three sliding grooves 16 are symmetrically arranged on the lower surface of the placement plate 13, and the sponge block 15 communicates with the sliding grooves 16. Nine humidity sensors 19 are symmetrically installed on the bottom wall of the inner wall of the placement slot 4. Nine indicator lights 20 are installed on the front surface of the box body 1.

[0023] In one embodiment, the box body 1 has a battery compartment 5 inside, and a storage battery 6 is installed inside the battery compartment 5. A charging port 12 is installed on one side of the box body 1, and the charging port 12 is electrically connected to the storage battery 6. The storage battery 6 provides power to the humidity sensor 19 and the indicator light 20, and the charging port 12 charges the storage battery 6.

[0024] In one embodiment, the top of the box body 1 is provided with a cover plate 7, and the two sides of the cover plate 7 are fixedly connected with first snap fasteners 11, and the two sides of the box body 1 are fixedly connected with first snap fasteners 10. The first snap fasteners 10 and the first snap fasteners 11 are engaged. The cover plate 7 is fixed by the first snap fasteners 10 and the first snap fasteners 11.

[0025] In one embodiment, nine springs 8 are symmetrically fixedly connected to the bottom of the cover plate 7, and a pressure plate 9 is fixedly connected to the bottom of the springs 8; the pressure plate 9 is used to press and fix the reagent container, and the springs 8 improve the stability of the pressure plate 9 in fixing the reagent container.

[0026] In one embodiment, two second female snap fasteners 17 are symmetrically installed on the front surface of the placement plate 13, and two second male snap fasteners 18 are symmetrically fixedly connected to the front surface of the box body 1. The second female snap fasteners 17 and the second male snap fasteners 18 are engaged. The position of the placement plate 13 is fixed by the setting of the second female snap fasteners 17 and the second male snap fasteners 18.

[0027] In operation: The second snap fastener 18 on the front surface of the box body 1 and the second snap fastener 17 on the placement plate 13 are released. The placement plate 13 is then removed from the placement groove 4. The sponge block 15 in the groove 14 is checked for dryness and integrity. If the sponge block 15 is damp or damaged, it is replaced with a new, dry sponge block 15. The placement plate 13 is then pushed back into the placement groove 4, and the second snap fastener 17 and the second snap fastener 18 are fastened to fix the position of the placement plate 13. The first snap fasteners 10 on both sides of the box body 1 and the first snap fasteners 11 on the cover plate 7 are released. The cover plate 7 is then lifted upwards. At this time, the spring 8 at the bottom of the cover plate 7 is in a naturally extended state, and the pressure plate 9 follows... Spring 8 is lifted, and the reagent containers required for nucleic acid extraction (such as centrifuge tubes containing lysis buffer and washing buffer) are placed into the nine reagent slots 2 on the upper surface of the box 1, ensuring that the bottom of each reagent container is in contact with the bottom wall of the reagent slot 2 to prevent the container from tilting. The cover 7 is then lowered, aligning the nine pressure plates 9 at the bottom of the cover 7 with the tops of the nine reagent containers. The cover 7 is gently pressed until the first female latch 10 of the box 1 and the first male latch 11 of the cover 7 are fully engaged, thus fixing the cover 7. During this process, spring 8 is elastically deformed by the pressure plates 9, pushing the pressure plates 9 tightly against the tops of the reagent containers, thus securing the reagent containers. The reagent tank 2 is securely fixed to prevent the container from shaking. If a reagent container leaks during reagent use, the leaked reagent will flow through the through-hole 3 on the bottom wall of reagent tank 2 into the placement tank 4 below, eventually seeping into the sponge block 15 in the groove 14 of the placement plate 13. When the sponge block 15 absorbs the leaked liquid, the humidity sensor 19 at the corresponding position on the bottom wall of the placement tank 4 will detect the humidity change. At this time, the indicator light 20, which is electrically connected to the humidity sensor 19 on the front surface of the box 1, will light up (e.g., a solid red light), providing real-time alert to the operator that a leak has occurred in a particular reagent tank 2. If the indicator light 20 illuminates, the operator must immediately stop the experiment. To perform the experiment, first loosen the first female buckle 10 and the first male buckle 11, lift the cover 7, remove the leaking reagent container, replace it with a new reagent, then loosen the second female buckle 17 and the second male buckle 18, pull out the placement plate 13, replace the damp sponge 15, wipe the placement plate 13 and the inner wall of the placement slot 4 with a clean paper towel to ensure no reagent residue remains, after processing, put the placement plate 13 back into the placement slot 4 and fix it, put in the new reagent container, and close the cover 7; after the experiment, take out all reagent containers, clean the reagent slot 2 and the through hole 3, ensure the inside of the box 1 is dry, and finally close the cover 7, fix the placement plate 13, and complete the storage.

[0028] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this utility model, and these should all be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A leak-proof nucleic acid extraction kit, characterized in that: The box includes a box body (1), on the upper surface of which nine reagent slots (2) are symmetrically arranged, and on the front surface of which a placement slot (4) is arranged. The bottom wall of the inner wall of the reagent slots (2) is provided with a through hole (3), which is connected to the placement slot (4). A placement plate (13) is slidably connected inside the placement slot (4). The upper surface of the placement plate (13) is provided with nine grooves (14), and a sponge block (15) is provided inside the grooves (14). The lower surface of the placement plate (13) is provided with three sliding grooves (16), which are connected to the sliding grooves (16). Nine humidity sensors (19) are symmetrically installed on the bottom wall of the placement slot (4). Nine indicator lights (20) are installed on the front surface of the box body (1).

2. The leak-proof nucleic acid extraction kit according to claim 1, characterized in that: The box (1) has a battery compartment (5) inside, and a storage battery (6) is installed inside the battery compartment (5). A charging port (12) is installed on one side of the box (1), and the charging port (12) is electrically connected to the storage battery (6).

3. The leak-proof nucleic acid extraction kit according to claim 1, characterized in that: The top of the box body (1) is provided with a cover plate (7), and the two sides of the cover plate (7) are fixedly connected with first buckle sub-buckles (11), and the two sides of the box body (1) are fixedly connected with first buckle female buckles (10), and the first buckle female buckle (10) is engaged with the first buckle sub-buckles (11).

4. The leak-proof nucleic acid extraction kit according to claim 3, characterized in that: Nine springs (8) are symmetrically fixedly connected to the bottom of the cover plate (7), and a pressure plate (9) is fixedly connected to the bottom of the springs (8).

5. The leak-proof nucleic acid extraction kit according to claim 1, characterized in that: Two second female buckles (17) are symmetrically installed on the front surface of the placement plate (13), and two second female buckles (18) are symmetrically fixedly connected to the front surface of the box body (1). The second female buckle (17) and the second female buckle (18) are engaged.

Images