A deep well plate for a nucleic acid extraction instrument

Abstract

The utility model relates to nucleic acid detection technical field, concretely for a kind of deep well plate for nucleic acid extractor, including deep well plate main body, first deep well unit, second deep well unit and third deep well unit, the deep well plate main body includes mounting seat, the upper surface of the mounting seat is evenly provided with multiple installation grooves, the upper surface of the installation groove is evenly provided with multiple limit grooves, the utility model is combined the hole site distribution of first / second / third deep well unit according to sample amount, experimental procedure freely by "limit groove+sliding type limiting cylinder" design, and three kinds of volume cover most nucleic acid extraction scene, solve the pain point of traditional deep well plate "fixed not adjustable", need not because volume demand multiple replacement entire deep well plate, once assembly can complete multi-step experiment, reduce operation frequency, reduce sample transfer link simultaneously, reduce the risk of cross-contamination, and each deep well unit is independently cleaned, further guarantee experiment purity.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid detection technology, specifically a deep-well plate for a nucleic acid extractor. Background Technology

[0002] In nucleic acid extraction experiments, deep-well plates are a core component of nucleic acid extractors, primarily used to hold samples and reagents and complete key steps such as lysis, washing, and elution. Currently available deep-well plates have significant technical limitations: fixed well positions and volumes: traditional deep-well plates are mostly integrated structures, with well distribution and single-well volume fixed at the factory, making it impossible to adjust according to experimental needs (such as differences in sample volume or volume changes in multiple steps). For example, when a large volume (approximately 1.5-2 mL) is required for lysis and a small volume (approximately 0.1-0.2 mL) for elution, different sizes of deep-well plates must be replaced, resulting in cumbersome operation. Low experimental efficiency: in multi-step experiments, frequent replacement of deep-well plates increases instrument downtime, and cross-contamination is prone to occur during sample transfer. Therefore, a new deep-well plate for nucleic acid extractors is proposed. Utility Model Content

[0003] In view of this, the present invention provides a deep well plate for a nucleic acid extractor to solve or alleviate the technical problems existing in the prior art, and at least provides a beneficial alternative.

[0004] The technical solution of this utility model is implemented as follows: a deep well plate for a nucleic acid extractor includes a deep well plate body, a first deep well unit, a second deep well unit, and a third deep well unit. The deep well plate body includes a mounting base. The upper surface of the mounting base is uniformly provided with a plurality of mounting grooves. The upper surface of the mounting groove is uniformly provided with a plurality of limiting grooves. The mounting grooves are connected to the limiting grooves. The first deep well unit, the second deep well unit, and the third deep well unit are all installed inside the mounting grooves.

[0005] More preferably, the first deep hole unit includes a first limiting cylinder, which is slidably connected to the inner wall of the limiting groove, and the bottom of the first limiting cylinder is connected to a first storage cylinder; the second deep hole unit includes a second limiting cylinder, which is slidably connected to the inner wall of the limiting groove, and the bottom of the second limiting cylinder is connected to a second storage cylinder; the third deep hole unit includes a third limiting cylinder, which is slidably connected to the inner wall of the limiting groove, and the bottom of the third limiting cylinder is connected to a third storage cylinder.

[0006] More preferably, the volume of the third deep hole unit is larger than that of the second deep hole unit, and the volume of the second deep hole unit is larger than that of the first deep hole unit.

[0007] More preferably, the volume of the second deep hole unit is twice that of the first deep hole unit, and the volume of the third deep hole unit is three times that of the first deep hole unit.

[0008] The present invention has the following advantages due to the adoption of the above technical solution:

[0009] This invention, through the design of "limiting groove + sliding limiting cylinder", allows for free combination of the well position distribution of the first / second / third deep well unit according to the sample volume and experimental steps. The three volumes cover most nucleic acid extraction scenarios, solving the pain point of traditional deep well plates being "fixed and unadjustable". It eliminates the need to replace the entire deep well plate multiple times due to volume requirements, and multi-step experiments can be completed in one assembly, reducing the number of operations, reducing sample transfer steps, lowering the risk of cross-contamination, and ensuring that each deep well unit is cleaned independently, further guaranteeing experimental purity.

[0010] The above overview is for illustrative purposes only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present invention will become readily apparent from the accompanying drawings and the following detailed description. Attached Figure Description

[0011] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0012] Figure 1 This is a structural diagram of the present invention;

[0013] Figure 2 This is a structural diagram of the mounting base of this utility model;

[0014] Figure 3 This is a structural diagram of the first deep hole unit of this utility model;

[0015] Figure 4 This is a structural diagram of the second deep hole unit of this utility model;

[0016] Figure 5 This is a structural diagram of the third deep hole unit of this utility model.

[0017] Reference numerals in the attached drawings: 1. Deep hole plate body; 11. Mounting base; 12. Mounting groove; 13. Limiting groove; 2. First deep hole unit; 21. First limiting cylinder; 22. First storage cylinder; 3. Second deep hole unit; 31. Second limiting cylinder; 32. Second storage cylinder; 4. Third deep hole unit; 41. Third limiting cylinder; 42. Third storage cylinder. Detailed Implementation

[0018] In the following description, only certain exemplary embodiments are briefly described. As those skilled in the art will recognize, the described embodiments can be modified in various ways without departing from the spirit or scope of this invention. Therefore, the drawings and description are considered exemplary in nature and not restrictive.

[0019] The embodiments of this utility model will now be described in detail with reference to the accompanying drawings.

[0020] like Figure 1-5 As shown, this utility model embodiment provides a deep well plate for a nucleic acid extractor, including a deep well plate body 1, a first deep well unit 2, a second deep well unit 3, and a third deep well unit 4. The deep well plate body 1 includes a mounting base 11, and a plurality of mounting grooves 12 are uniformly formed on the upper surface of the mounting base 11. A plurality of limiting grooves 13 are uniformly formed on the upper surface of the mounting grooves 12. The mounting grooves 12 and the limiting grooves 13 are connected. The first deep well unit 2, the second deep well unit 3, and the third deep well unit 4 are all installed inside the mounting grooves 12.

[0021] In one embodiment, the first deep hole unit 2 includes a first limiting cylinder 21, which is slidably connected to the inner wall of the limiting groove 13, and the bottom of the first limiting cylinder 21 is connected to a first storage cylinder 22. The second deep hole unit 3 includes a second limiting cylinder 31, which is slidably connected to the inner wall of the limiting groove 13, and the bottom of the second limiting cylinder 31 is connected to a second storage cylinder 32. The third deep hole unit 4 includes a third limiting cylinder 41, which is slidably connected to the inner wall of the limiting groove 13, and the bottom of the third limiting cylinder 41 is connected to a third storage cylinder 42.

[0022] In one embodiment, the volume of the third deep hole unit 4 is greater than that of the second deep hole unit 3, and the volume of the second deep hole unit 3 is greater than that of the first deep hole unit 2.

[0023] In one embodiment, the volume of the second deep hole unit 3 is twice that of the first deep hole unit 2, and the volume of the third deep hole unit 4 is three times that of the first deep hole unit 2.

[0024] In operation, the following steps are performed: the first limiting cylinder 21 of the first deep well unit 2, the second limiting cylinder 31 of the second deep well unit 3, and the third limiting cylinder 41 of the third deep well unit 4 are slidably embedded into the limiting groove 13 of the mounting base 11, ensuring that the limiting cylinders are in contact with the inner wall of the mounting groove 12. The deep well units are arranged in the order of experimental steps to facilitate subsequent sample addition and instrument identification. Samples and lysis reagents are added to the third storage cylinder 42 of the third deep well unit 4, washing reagents are added to the second storage cylinder 32 of the second deep well unit 3, and elution buffer (volume matching the requirements of each step) is added to the first storage cylinder 22 of the first deep well unit 2. After sample addition, each storage cylinder is checked for leakage. The deep well plate body 1 with the assembled deep well units is placed in the standard position of the nucleic acid extractor, and the extraction program is started. After the program ends, the deep well plate is removed, and each deep well unit is slid out from the limiting groove 13. The elution products in the first storage cylinder 22 are collected.

[0025] The above description is merely a specific embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any person skilled in the art can easily conceive of various variations or substitutions within the technical scope disclosed in this utility model, and these should all be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the protection scope of the claims.

Claims

1. A deep-well plate for a nucleic acid extractor, characterized in that: The device includes a deep hole plate body (1), a first deep hole unit (2), a second deep hole unit (3), and a third deep hole unit (4). The deep hole plate body (1) includes a mounting base (11). The upper surface of the mounting base (11) is uniformly provided with a plurality of mounting grooves (12). The upper surface of the mounting grooves (12) is uniformly provided with a plurality of limiting grooves (13). The mounting grooves (12) and the limiting grooves (13) are connected. The first deep hole unit (2), the second deep hole unit (3), and the third deep hole unit (4) are all installed inside the mounting grooves (12).

2. The deep-well plate for a nucleic acid extractor according to claim 1, characterized in that: The first deep hole unit (2) includes a first limiting cylinder (21), which is slidably connected to the inner wall of the limiting groove (13). The bottom of the first limiting cylinder (21) is connected to a first storage cylinder (22). The second deep hole unit (3) includes a second limiting cylinder (31), which is slidably connected to the inner wall of the limiting groove (13). The bottom of the second limiting cylinder (31) is connected to a second storage cylinder (32). The third deep hole unit (4) includes a third limiting cylinder (41), which is slidably connected to the inner wall of the limiting groove (13). The bottom of the third limiting cylinder (41) is connected to a third storage cylinder (42).

3. A deep-well plate for a nucleic acid extractor according to claim 1, characterized in that: The volume of the third deep hole unit (4) is greater than that of the second deep hole unit (3), and the volume of the second deep hole unit (3) is greater than that of the first deep hole unit (2).

4. A deep-well plate for a nucleic acid extractor according to claim 1, characterized in that: The second deep hole unit (3) has a volume twice that of the first deep hole unit (2), and the third deep hole unit (4) has a volume three times that of the first deep hole unit (2).

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