A rapid detection device for pesticide residues in chilli pepper

Abstract

The utility model provides a kind of quick detection device of pepper pesticide residue, it is related to pepper planting technical field, including bearing support, detection tube, pipe cover and reagent bin, the bearing support includes a handheld rod and at least one detection tube of the sleeve of insertion, the sleeve is connected in the lower end of the handheld rod, colorimetric area is provided on the outer wall of sleeve, the detection tube is transparent tube body detachably inserted in sleeve, the pipe cover is detachably installed in the pipe mouth of detection tube, the reagent bin is pre-filled detection reagent, the reagent bin is detachably installed in pipe cover bottom, the utility model is convenient for farmer to carry out rapid preliminary screening to pesticide residue in field.

Description

Technical Field

[0001] This utility model relates to the field of chili pepper cultivation technology, specifically to a rapid detection device for pesticide residues in chili peppers. Background Technology

[0002] For chili pepper growers, field pesticide management presents numerous uncertainties, as weather and season can affect the degradation rate of pesticides. Organophosphates and carbamates are the two most widely used insecticides in chili pepper cultivation, each with a legally mandated "safe interval." However, this period is influenced by various environmental factors such as weather, season, sunlight, and temperature. Relying solely on calendars to estimate harvest time is highly uncertain, making the theoretical safe interval only a reference. Before investing significant manpower in harvesting, growers need a simple method to conduct a pre-test of the chilies to initially assess pesticide residue levels, providing a basis for determining the optimal harvest time. Furthermore, on-site assessment of pesticide residues after application helps growers understand the overall effectiveness of the pesticides, providing a reference for subsequent precise pesticide management.

[0003] However, professional laboratory testing is time-consuming and costly, which cannot meet the need for such immediacy. Therefore, it is necessary to develop a rapid testing device that is specifically adapted to the shape of chili peppers, has a highly integrated operation process, and is suitable for growers to use on the production site. Utility Model Content

[0004] To overcome the problems in the prior art, this utility model provides a rapid detection device for pesticide residues in chili peppers, which makes it convenient for farmers to quickly and initially screen for organophosphate and carbamate pesticide residues in the field.

[0005] A rapid detection device for pesticide residues in chili peppers includes a support frame, a detection tube, a tube cap, and a reagent compartment. The support frame includes a handheld rod and at least one sleeve for inserting the detection tube. The sleeve is connected to the lower end of the handheld rod, and a colorimetric area is provided on the outer wall of the sleeve. The detection tube is a transparent tube that can be detachably inserted into the sleeve. The tube cap is detachably installed at the opening of the detection tube. The reagent compartment is pre-filled with detection reagents and is detachably installed at the bottom of the tube cap.

[0006] Furthermore, the bottom of the tube cap is provided with an external thread, and the inner wall of the opening of the reagent compartment is provided with an internal thread that mates with the external thread.

[0007] Furthermore, the reagent compartment is a disposable reagent compartment, and the detection reagent is a lyophilized powder.

[0008] Furthermore, the card sleeve is a hollow semi-cylindrical structure, and the inner surface of the card sleeve is provided with a matte white coating.

[0009] Furthermore, the ferrule is evenly distributed around the lower end of the handheld lever.

[0010] Compared with the prior art, the beneficial effects of this utility model are: 1. The detection tube of this device is adapted to the slender shape of chili peppers, which can achieve full wetting and rinsing of the entire surface of the chili pepper with less eluent. Combined with the shaking operation of the handheld rod, it improves the efficiency and effect of sample pretreatment. The handheld rod, card holder and colorimetric area are integrated into one unit, and all operations and observations can be completed on a single device. There is no need to carry separate colorimetric cards and other accessories, which is very suitable for field use.

[0011] 2. The disposable reagent compartment is pre-filled with stable lyophilized powder reagents, which solves the problem of traditional liquid reagents being difficult to preserve and ensures the standardization of the dosage for each test, eliminating human error; the colorimetric design of the cassette enhances color contrast, reduces the probability of visual misreading caused by complex ambient light, and makes the test results easier to observe. Attached Figure Description

[0012] To clearly illustrate the technical solutions in the embodiments of this utility model, the accompanying drawings used in the description of the embodiments are explained.

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the load-bearing support structure of this utility model; Figure 3 This is a schematic diagram of the detection tube structure of this utility model; Figure 4 This is a schematic diagram of the reagent compartment structure of this utility model.

[0014] Reference numerals: 1-Support bracket, 11-Handheld rod, 12-Clip sleeve, 13-Colorimetric area, 2-Detection tube, 3-Tube cap, 4-Reagent compartment. Detailed Implementation

[0015] To make the objectives, technical solutions, and beneficial effects of this utility model clearer, the preferred embodiments of this utility model will be described in detail below with reference to the accompanying drawings, so as to facilitate the understanding of those skilled in the art.

[0016] See Figure 1-3This utility model proposes a rapid detection device for pesticide residues in chili peppers, including a support bracket 1, a detection tube 2, a tube cap 3, and a reagent compartment 4. The support bracket 1 is an integrated structure with a slender handheld rod 11 at its upper part, which is convenient for users to hold and shake with one hand. The top of the handheld rod 11 preferably has a through hole, which can be used for hanging with a rope or holding with fingers. The lower end of the handheld rod 11 is connected to several retaining sleeves 12. These retaining sleeves 12 are evenly distributed circumferentially around the central axis of the handheld rod 11. Each retaining sleeve 12 is a hollow semi-cylindrical structure, and its inner diameter is adapted to the outer diameter of the detection tube 2 for stable insertion and fixation of the detection tube 2. Limiting points are set on the inner wall of the retaining sleeve 12 to lock the detection tube 2.

[0017] To facilitate result comparison, each cassette 12 has a colorimetric area 13 printed or pasted on its outer wall. The colorimetric area 13 contains at least two color standards, such as a dark patch representing a value below the reference threshold and a light patch representing a value above the reference threshold. Meanwhile, the inner surface of the cassette 12 is provided with a matte white coating to provide an ideal neutral observation background for the color change of the liquid in the detection tube 2, thereby improving the convenience of observation.

[0018] The detection tube 2 is a transparent plastic or glass tube with an open opening and a closed bottom. Its inner cavity size is adapted to accommodate a regular-sized chili pepper.

[0019] The cap 3 is used to seal the opening of the detection tube 2 to prevent liquid from splashing out when shaken. The bottom of the cap 3, that is, the end that extends into the detection tube 2, is provided with external threads.

[0020] The reagent compartment 4 is a small, open-top cup-shaped container. The interior of the reagent compartment 4 is pre-filled with lyophilized powdered test reagents. In this embodiment, the test reagents are formulated based on the principle of acetylcholinesterase inhibition. Their main components include acetylcholinesterase and chromogenic substrate. These components are made into lyophilized powders to ensure their storage stability at room temperature. The inner wall of the opening of the reagent compartment 4 is provided with an internal thread that matches the external thread at the bottom of the tube cap 3. By rotating, the reagent compartment 4 can be easily installed on or removed from the tube cap 3. The reagent compartment 4 is designed as a disposable consumable, can be individually packaged, and sold as a panel with the device.

[0021] Work process: This device is based on an enzyme inhibition colorimetric method. During detection, pesticides on the surface of the chili peppers are first eluted and reacted with an enzyme. If either of these pesticides is present, the enzyme activity is inhibited. Then, a chromogenic substrate is added. Active enzymes catalyze the hydrolysis of the substrate to produce a colored substance, while inhibited enzymes can only partially or no longer catalyze the reaction. Therefore, the level of pesticide residue can be determined by observing the final color intensity. A darker color indicates higher enzyme activity and lower pesticide residue; a lighter color or no color change indicates inhibited enzyme activity and higher pesticide residue.

[0022] Specific usage steps: Preparation: The user takes a disposable reagent compartment 4, rotates it to install it at the bottom of the tube cap 3, adds the specified amount of buffer solution, such as phosphate buffer, to the empty test tube 2, and then tightens the tube cap 3 containing the reagent compartment 4 onto the opening of the test tube 2. Sampling and elution: Collect the peppers to be tested by area, put the whole pepper to be tested into the test tube 2, and the user holds the hand rod 11 of the support bracket 1 and shakes the entire device vigorously for 30-60 seconds to allow the buffer solution to fully wash the surface of the pepper and elute any possible organophosphate or carbamate pesticide residues into the liquid. Color reaction: After shaking, loosen the tube cap 3 so that the reagent chamber 4 connected to the tube cap falls into the liquid in the detection tube 2, or pour the lyophilized powder reagent into the liquid. The lyophilized powder reagent in the reagent chamber will quickly dissolve and react. Observation results: After standing for 3 minutes, the user compares the color of the liquid in the test tube 2 with the colorimetric area 13 on the outer wall of the adjacent sleeve 12 to obtain a preliminary screening result on the pesticide residue of the chili sample. If the color result is within the safe range, such as dark blue, it indicates that harvesting can be carried out.

[0023] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A rapid detection device for pesticide residues in chili peppers, characterized in that, The device includes a support bracket (1), a detection tube (2), a tube cap (3), and a reagent compartment (4). The support bracket (1) includes a hand handle (11) and at least one sleeve (12) for inserting the detection tube (2). The sleeve (12) is connected to the lower end of the hand handle (11). A colorimetric area (13) is provided on the outer wall of the sleeve (12). The detection tube (2) is a transparent tube that can be detachably inserted into the sleeve (12). The tube cap (3) is detachably installed at the opening of the detection tube (2). The reagent compartment (4) is pre-filled with detection reagents and is detachably installed at the bottom of the tube cap (3).

2. The rapid detection device for pesticide residues in chili peppers according to claim 1, characterized in that, The bottom of the tube cap (3) is provided with an external thread, and the inner wall of the opening of the reagent compartment (4) is provided with an internal thread that mates with the external thread.

3. The rapid detection device for pesticide residues in chili peppers according to claim 1, characterized in that, The reagent compartment (4) is a disposable reagent compartment, and the test reagent is a lyophilized powder.

4. The rapid detection device for pesticide residues in chili peppers according to claim 1, characterized in that, The sleeve (12) is a hollow semi-cylindrical structure, and the inner surface of the sleeve (12) is provided with a matte white coating.

5. The rapid detection device for pesticide residues in chili peppers according to claim 1, characterized in that, The sleeve (12) is evenly distributed around the lower end of the hand handle (11).

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