Multicolor fluorescent detection kit

Abstract

The utility model discloses a multicolor fluorescence joint detection kit, including box body, sample pad, combination pad, detection pad and absorbent pad still include: dustproof mechanism: dustproof mechanism sets up on the top outer wall of box body, is equipped with observation port and sample hole on the top outer wall of box body, is provided with first antifouling cover on the inner wall of observation port, is provided with second antifouling cover on the inner wall of sample hole, the storage mechanism: the storage mechanism sets up on the inner wall of sample hole, is equipped with storage groove on the inner wall of sample hole, is provided with storage cover at the notch of storage groove, is provided with connecting assembly on the outer wall of second antifouling cover. The utility model discloses multicolor fluorescence joint detection kit has reduced test paper pollution risk, improves the effect of detection accuracy.

Description

Technical Field

[0001] This utility model relates to the field of medical testing technology, and in particular to a multicolor fluorescence detection kit. Background Technology

[0002] Immunochromatography, as a rapid diagnostic technique, has been widely used in clinical diagnosis, food safety testing, and environmental monitoring since its emergence in the 1990s. Its basic principle is to immobilize specific antibodies on a reaction membrane, allowing the sample to move forward via capillary action and specifically bind to the immobilized antibodies. If immunogold is used, the binding area will display color, thus achieving specific immunodiagnosis.

[0003] However, in traditional multicolor fluorescent assay kits, the test strips are in direct contact with the air inside the packaging box, and the sample application well and observation port lack dustproof structures, making them susceptible to contamination and thus affecting the accuracy of the test results.

[0004] For example, in laboratory or field testing environments, if there are impurities such as dust, fibers, or microorganisms in the air, these contaminants may fall onto the surface of the test strip through the sample application hole or adhere to the observation port, affecting the reading of the fluorescence signal. In addition, during transportation or storage, if the packaging box is squeezed or shaken, the test strip may also adsorb impurities due to contact with the air, leading to false positives or false negatives in the test results, affecting the accuracy and reliability of the test, and thus causing serious misleading to clinical diagnosis or environmental monitoring. Utility Model Content

[0005] This utility model discloses a multicolor fluorescence detection kit, which aims to solve the technical problem that the test strips are in direct contact with the air inside the packaging box, and the sample application hole and observation port lack dustproof structure, making them easily contaminated and thus affecting the accuracy of the test results.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A multicolor fluorescent assay kit includes a housing, a sample pad, a conjugate pad, a detection pad, and an absorbent pad, and further includes:

[0008] Dustproof mechanism: The dustproof mechanism is installed on the top outer wall of the box body. An observation port and a sample addition hole are provided on the top outer wall of the box body. A first anti-fouling cover is provided on the inner wall of the observation port, and a second anti-fouling cover is provided on the inner wall of the sample addition hole.

[0009] Storage mechanism: The storage mechanism is provided on the inner wall of the sample dispensing hole. A storage groove is provided on the inner wall of the sample dispensing hole. A storage cover is provided at the opening of the storage groove. A connecting component is provided on the outer wall of the second anti-fouling cover.

[0010] In this case, the dustproof mechanism is located on the top outer wall of the box, including a first and a second anti-fouling cover on the observation port and sample application port. This prevents dust, fibers, and other contaminants from entering the box and contaminating the test strips. The first anti-fouling cover is made of polymethyl methacrylate (PMMA), a material with excellent optical transparency that does not interfere with the detection and observation of fluorescence signals. It is also chemically inert, does not easily react with common biochemical reagents, and has a smooth surface that does not easily adsorb contaminants. The storage mechanism provides a dedicated storage location for the second anti-fouling cover through a storage groove and a storage cover on the inner wall of the sample application port, preventing contamination or loss caused by careless placement. This dual-protection design is particularly suitable for laboratory or on-site testing environments, significantly reducing false positive or false negative results caused by air pollutants, improving testing accuracy, optimizing the operating process, and making the testing process more standardized.

[0011] As described above, a multicolor fluorescent assay kit includes a box, a sample pad, a conjugate pad, a detection pad, and an absorbent pad. It also includes: a dustproof mechanism: the dustproof mechanism is located on the top outer wall of the box, and the top outer wall of the box has an observation port and a sample application hole. A first anti-fouling cover is provided on the inner wall of the observation port, and a second anti-fouling cover is provided on the inner wall of the sample application hole; a storage mechanism: the storage mechanism is located on the inner wall of the sample application hole, and the inner wall of the sample application hole has a storage groove. A storage cover is provided at the opening of the storage groove, and a connecting component is provided on the outer wall of the second anti-fouling cover. The multicolor fluorescent assay kit provided by this invention has the technical effect of reducing the risk of test strip contamination and improving detection accuracy. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the overall structure of a multicolor fluorescence detection kit proposed in this utility model.

[0013] Figure 2 This is a schematic diagram of the storage mechanism of a multicolor fluorescence detection kit proposed in this utility model.

[0014] Figure 3 This is a cross-sectional structural diagram of a multicolor fluorescence detection kit proposed in this utility model.

[0015] In the attached diagram: 1. Box body; 2. First anti-fouling cover; 3. Second anti-fouling cover; 4. Protrusion; 5. Storage cover; 6. Sample dispensing hole; 7. Storage pad; 8. Baffle; 9. Connecting rod; 10. Sliding joint; 11. Desiccant; 12. Sample pad; 13. Binding pad; 14. Detection pad; 15. Detection line; 16. Quality control line; 17. Absorbent pad; 18. Support column; 19. Mounting sleeve; 20. Fixing groove; 21. Steel ball; 22. Limiting groove. Detailed Implementation

[0016] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. The components of the embodiments of this application described and marked in the accompanying drawings can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of this application provided in the accompanying drawings is not intended to limit the scope of the claimed application, but merely represents selected embodiments of this application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0017] The multicolor fluorescence detection kit disclosed in this utility model is mainly used in scenarios where the test strips are in direct contact with air inside the packaging box, and the sample application port and observation port lack dustproof structure, making them susceptible to contamination and thus affecting the accuracy of the test results.

[0018] Reference Figure 1 , Figure 2 and Figure 3 A multicolor fluorescence detection kit includes a box body 1, a sample pad 12, a conjugate pad 13, a detection pad 14, and an absorbent pad 17. It also includes: a dustproof mechanism: the dustproof mechanism is located on the top outer wall of the box body 1, and the top outer wall of the box body 1 has an observation port and a sample application hole 6. A first anti-fouling cover 2 is provided on the inner wall of the observation port, and a second anti-fouling cover 3 is provided on the inner wall of the sample application hole 6; a storage mechanism: the storage mechanism is located on the inner wall of the sample application hole 6, and the inner wall of the sample application hole 6 has a storage groove. A storage cover 5 is provided at the opening of the storage groove, and a connecting component is provided on the outer wall of the second anti-fouling cover 3.

[0019] Sample pad 12, binding pad 13, detection pad 14 and absorption pad 17 are arranged sequentially on the bottom inner wall of the box 1. The detection pad 14 is provided with a quality control line 16 and four detection lines 15.

[0020] Desiccant 11 is provided on the inner wall of opposite sides of box 1. The addition of desiccant 11 on both sides inside box 1 effectively controls the humidity environment inside box 1. The desiccant 11 is symmetrically arranged on opposite inner walls to form a uniform dehumidification effect, preventing the test paper from getting damp and deteriorating, and ensuring the stability of the test paper.

[0021] Specifically, the sample pad 12, binding pad 13, detection pad 14, and absorption pad 17 are arranged sequentially at the bottom of the housing 1 to form a complete chromatography channel. The detection pad 14 is equipped with a quality control line 16 and four detection lines 15 to achieve simultaneous detection of multiple indicators. This layout optimizes chromatography efficiency and reaction uniformity. The four detection lines 15 can correspond to the CRP detection line 15, SAA detection line 15, PCT detection line 15, and HBP detection line 15, respectively. Combined with multicolor fluorescence technology, high-throughput joint detection is achieved. The quality control line 16 provides validity verification of the detection process to ensure the reliability of the results.

[0022] In practical applications, each functional pad is installed using precision cutting and positioning technology to ensure tight contact without gaps. The capture probes on the detection line 15 are precisely arranged using microarray technology. The four detection lines 15 are spaced evenly to avoid cross-reaction. The quality control line 16 is located at the end of the detection line 15 and adopts a universal capture molecule design. After the sample is added through the sample application hole 6, it diffuses evenly through the sample pad 12, binds to the marker when passing through the binding pad 13, and then undergoes a specific reaction on the detection pad 14. Finally, excess liquid is collected by the absorption pad 17. This standardized layout greatly improves the consistency and repeatability of the detection and is suitable for mass production and quality control.

[0023] In the specific implementation process, the dustproof mechanism is set on the top outer wall of the box 1, including the first anti-fouling cover 2 and the second anti-fouling cover 3 on the observation port and sample application hole 6. It can prevent dust, fibers and other contaminants from entering the box 1 and contaminating the test strip. The first anti-fouling cover 2 is made of polymethyl methacrylate, which has excellent optical transparency, will not interfere with the detection and observation of fluorescence signals, and is chemically inert, does not easily react with common biochemical reagents, and has a smooth surface that does not easily adsorb contaminants. The storage mechanism provides a dedicated storage location for the second anti-fouling cover 3 through the storage groove and storage cover 5 on the inner wall of the sample application hole 6, avoiding contamination or loss caused by random placement of the anti-fouling cover. This dual protection design is particularly suitable for laboratory or on-site testing environments, which can significantly reduce false positive or false negative results caused by air pollutants, improve the accuracy of detection, optimize the operation process, and make the detection process more standardized.

[0024] Reference Figure 2 and Figure 3 In a preferred embodiment, the connecting assembly includes a support column 18, which is disposed on the top outer wall of the box 1. A baffle 8 is provided at one end of the support column 18. An mounting sleeve 19 is provided on the circumferential outer wall of the support column 18. A limiting component is provided on the bottom outer wall of the mounting sleeve 19. A protrusion 4 and a connecting rod 9 are provided on the circumferential outer wall of the mounting sleeve 19. One end of the connecting rod 9 is connected to the second anti-fouling cover 3.

[0025] Specifically, the support column 18 is fixed to the top of the box 1, and a baffle 8 is set at one end as a limit. The mounting sleeve 19 on the outer circumference is fixed in position by the limiting component. One end of the connecting rod 9 is connected to the second anti-fouling cover 3. This design makes the opening and closing of the second anti-fouling cover 3 smoother. The opening and closing state of the second anti-fouling cover 3 can be adjusted by the protrusion 4. By using the limiting component at the bottom of the mounting sleeve 19 to cooperate with the limiting groove 22 on the box 1, the second anti-fouling cover 3 can be positioned at multiple angles to adapt to different operating needs and improve the convenience of operation.

[0026] Reference Figure 2 In a preferred embodiment, a storage pad 7 is provided on the bottom inner wall of the storage slot, and a sliding slit 10 is provided on the outer wall of the storage cover 5. The height of the sliding slit 10 is the same as the thickness of the connecting rod 9.

[0027] It should be noted that the storage pad 7, made of medical non-woven fabric, is attached to the bottom of the storage slot, providing a clean temporary storage location for the second anti-fouling cover 3, effectively preventing bottom contamination. The design of precisely matching the height of the sliding seam 10 with the thickness of the connecting rod 9 ensures that the anti-fouling cover can slide smoothly along the predetermined trajectory, realizing one-click storage and retrieval functions. During operation, simply rotate the protrusion 4 to push the second anti-fouling cover 3, which is connected to one end of the connecting rod 9, into the storage slot along the sliding seam 10, and then reverse the operation to quickly retrieve it. The entire process requires no additional adjustment or strenuous operation. This user-friendly design avoids the risk of contamination caused by the random placement of the anti-fouling cover and maintains a clean and orderly working environment.

[0028] Reference Figure 3 In a preferred embodiment, the limiting component includes a steel ball 21. A fixing groove 20 is provided on the bottom outer wall of the mounting sleeve 19. The steel ball 21 is connected to the bottom inner wall of the fixing groove 20 by a spring. A plurality of equally spaced limiting grooves 22 are provided on the top outer wall of the box body 1. The steel ball 21 and the limiting grooves 22 are used in conjunction.

[0029] Specifically, the fixing groove 20 is a cylindrical groove with a built-in compression spring and steel ball 21. The limiting groove 22 is evenly distributed along the bottom circumference of the support column 18. When the second anti-fouling cover 3 is rotated, the steel ball 21 is pressed into the fixing groove 20 to compress the spring. After passing the protrusion between the limiting grooves 22, it bounces into the next limiting groove 22, producing a positioning effect. The spring preload is precisely calculated to ensure stable positioning without being too strenuous. Users can fix the second anti-fouling cover 3 at multiple preset angles as needed, improving the ease of operation.

[0030] Working principle: In use, first open the second anti-fouling cover 3, and add the sample to be tested to the sample pad 12 through the sample application hole 6. Under the action of capillary, the sample passes through the conjugation pad 13 and the detection pad 14 in sequence, and reacts with the specific antibody on the detection pad 14 to form a fluorescent signal complex. The quality control line 16 and the four detection lines 15 set on the detection pad 14 can perform multi-index simultaneous detection on the sample to ensure the comprehensiveness and accuracy of the test results. Excess liquid is absorbed by the absorption pad 17. During the test, the dustproof mechanism effectively prevents dust and other contaminants from entering the inside of the box 1 to ensure the cleanliness of the test environment.

[0031] The above description is merely a preferred embodiment of this utility model, but the protection scope of this utility model is not limited thereto. The substitutions may be replacements of some structures, devices, or method steps, or they may be complete technical solutions. Equivalent substitutions or modifications made based on the technical solution and inventive concept of this utility model should all be covered within the protection scope of this utility model.

Claims

1. A multicolor fluorescence detection kit, comprising a housing (1), a sample pad (12), a conjugate pad (13), a detection pad (14), and an absorbent pad (17), characterized in that, Also includes: Dustproof mechanism: The dustproof mechanism is set on the top outer wall of the box body (1). An observation port and a sample addition hole (6) are opened on the top outer wall of the box body (1). A first anti-fouling cover (2) is set on the inner wall of the observation port, and a second anti-fouling cover (3) is set on the inner wall of the sample addition hole (6). Storage mechanism: The storage mechanism is set on the inner wall of the sample feeding hole (6). A storage groove is opened on the inner wall of the sample feeding hole (6). A storage cover (5) is set at the opening of the storage groove. A connecting component is set on the outer wall of the second anti-fouling cover (3).

2. The multicolor fluorescence detection kit according to claim 1, characterized in that, The connecting assembly includes a support column (18), which is disposed on the top outer wall of the box (1). A baffle (8) is provided at one end of the support column (18). An installation sleeve (19) is provided on the circumferential outer wall of the support column (18). A limit component is provided on the bottom outer wall of the installation sleeve (19). A protrusion (4) and a connecting rod (9) are provided on the circumferential outer wall of the installation sleeve (19). One end of the connecting rod (9) is connected to the second anti-fouling cover (3).

3. The multicolor fluorescence detection kit according to claim 2, characterized in that, A storage pad (7) is provided on the bottom inner wall of the storage slot, and a sliding slit (10) is provided on the outer wall of the storage cover (5). The height of the sliding slit (10) is the same as the thickness of the connecting rod (9).

4. The multicolor fluorescence detection kit according to claim 3, characterized in that, The limiting component includes a steel ball (21), and a fixing groove (20) is provided on the bottom outer wall of the mounting sleeve (19). The steel ball (21) is connected to the bottom inner wall of the fixing groove (20) by a spring. Several equally spaced limiting grooves (22) are provided on the top outer wall of the box (1).

5. The multicolor fluorescence detection kit according to claim 4, characterized in that, The steel ball (21) is used in conjunction with the limiting groove (22).

6. The multicolor fluorescence detection kit according to claim 1, characterized in that, The sample pad (12), the binding pad (13), the detection pad (14) and the absorption pad (17) are arranged sequentially on the bottom inner wall of the box (1). The detection pad (14) is provided with a quality control line (16) and four detection lines (15).

7. The multicolor fluorescence detection kit according to claim 6, characterized in that, Desiccant (11) is provided on the inner wall of each opposite side of the box (1).

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