A detection card for detecting content of ferulic acid in traditional Chinese medicinal materials

Abstract

The utility model relates to detection card technical field, and disclose a detection card of detecting ferulic acid content in traditional Chinese medicinal material, the inside joint of arc flow groove has sample absorption auxiliary strip, the bottom fixed connection of sample absorption auxiliary strip has sample pad, the lateral surface fixed connection of sample pad has gold mark pad, the lateral surface fixed mounting of gold mark pad has NC membrane, the lateral surface fixed mounting of NC membrane has absorption pad, the middle part of NC membrane is provided with detection line T coated with ferulic acid antigen and quality control line C coated with sheep anti - mouse second antibody respectively. The detection card of detecting ferulic acid content in traditional Chinese medicinal material, and the liquid of introduction is flowed into the inside communication of arc flow groove, utilizes the sample absorption auxiliary strip of arc flow groove inside joint and carries out absorption, and chromatography to sample pad through sample absorption auxiliary strip layer, and then can absorb the sample liquid of super capacity, guarantee sample liquid fully and gold mark pad combine, and then guarantee the precision of detection data.

Description

Technical Field

[0001] This utility model relates to the field of detection card technology, specifically a detection card for detecting the content of ferulic acid in traditional Chinese medicine. Background Technology

[0002] Ferulic acid is found in many traditional Chinese medicines, such as angelica, chuanxiong, danshen, sage, and coffee beans. Numerous studies have shown that it is very effective in preventing and treating cardiovascular diseases. It is a phenolic acid widely found in plants, where it combines with polysaccharides and proteins in the cell wall to form the cell wall skeleton. It rarely exists in a free form. Ferulic acid has the effects of dispersing blood stasis, eliminating stagnation, resolving masses, promoting qi circulation, and relieving pain, and has certain medicinal value. The content of ferulic acid in traditional Chinese medicines is detected by a test card.

[0003] Current test strips typically use colloidal gold-labeled antibodies and consist of a base plate, an NC membrane, a sample pad, a conjugate pad, absorbent paper, and a cartridge. The sample pad delivers the sample to other parts of the test strip, serving a pretreatment function. The conjugate pad contains the labeled material, affecting label release and detection sensitivity. The NC membrane is the primary driving force for the chromatographic reaction and the site of immune response and signal testing. The absorbent pad, located at the end of the strip, helps maintain the flow rate of the liquid on the membrane and prevents sample backflow. During sample dropper insertion through the cartridge, the storage size of the sample dropper and the absorption rate of the sample pad, combined with impurity removal on the sample pad and upward chromatographic flow, can prevent the dropped sample from fully binding with the gold-labeled pad, thus affecting the final test results.

[0004] Based on this, the present invention provides a detection card for detecting the content of ferulic acid in traditional Chinese medicine. Utility Model Content

[0005] To address the shortcomings of existing technologies, this invention provides a detection card for detecting ferulic acid content in traditional Chinese medicine. It features a waist-shaped sample dropper combined with a sample absorption strip to ensure sufficient sample drop volume without overflow, thus solving the problems mentioned in the background technology.

[0006] This utility model provides the following technical solution: a detection card for detecting the ferulic acid content in traditional Chinese medicine, comprising an upper card shell and a lower card shell. The upper card shell has a sample drop inlet hole, which includes a spherical groove, a cylindrical groove, a square guide groove, and an arc-shaped guide groove. The bottom of the spherical groove is provided with a cylindrical groove, and the bottom of the cylindrical groove is provided with a spherical groove. The spherical groove, cylindrical groove, and spherical groove are connected. The inner wall of the cylindrical groove is provided with a square guide groove. The inner wall of the spherical groove two is provided with an arc-shaped guide channel. The square guide channel and the arc-shaped guide channel are connected. A sample absorption auxiliary strip is engaged inside the arc-shaped guide channel. A sample pad is fixedly connected to the bottom of the sample absorption auxiliary strip. A gold label pad is fixedly connected to the side of the sample pad. An NC membrane is fixedly installed on the side of the gold label pad. An absorption pad is fixedly installed on the side of the NC membrane. The middle part of the NC membrane is provided with a detection line T coated with ferulic acid antigen and a quality control line C coated with goat anti-mouse secondary antibody.

[0007] Preferably, a card interface is provided at each of the four corners of the lower surface of the upper card shell, a card receiving post is provided inside the card interface, and the lower card shell is fixedly installed at the bottom of the card receiving post.

[0008] Preferably, the bottom of the upper casing has two card interfaces, and the two card interfaces are provided with card receiving posts.

[0009] Preferably, a first fitting shell is fixedly installed at the bottom of the lower casing, a sample pad is fitted inside the cavity of the first fitting shell, and two first abutting protrusions are fixedly installed inside the first fitting shell, with the inner sides of the two first abutting protrusions abutting against the outer side of the sample pad.

[0010] Preferably, two fitting shells are fixedly installed at the bottom of the lower housing, and two abutting protrusions are fixedly installed inside the two fitting shells. The inner side of the abutting protrusions abuts against the outer side of the absorbent pad.

[0011] Preferably, the upper casing has an observation slot located at the center.

[0012] Preferably, the upper and lower retainers cooperate to form a hollow cuboid, the length and width of the upper and lower retainers are the same, and the gold label pad is coated with colloidal gold particles adsorbed with anti-ferulic acid monoclonal antibodies or latex microspheres (or luminescent microspheres) chemically bonded with anti-ferulic acid monoclonal antibodies.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] This test card for detecting ferulic acid content in traditional Chinese medicine uses a spherical groove, a cylindrical groove, and a spherical groove to form a waist-shaped droplet orifice. During the droplet process, the sample solution accumulates in the lower hemisphere of the spherical groove. The accumulated sample solution then enters the spherical groove through the cylindrical groove. The upper hemisphere of the spherical groove further expands the droplet area, ensuring sufficient sample solution volume. Simultaneously, a square guide channel connecting the cylindrical and spherical grooves provides auxiliary liquid guidance. The introduced liquid then flows into a connected arc-shaped guide channel, where it is absorbed by a sample absorption strip. The sample solution then precipitates onto the sample pad, absorbing excess sample solution and ensuring thorough binding with the gold standard pad, thus guaranteeing accurate test data. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the device of this utility model;

[0016] Figure 2 This utility model Figure 1 A schematic diagram of the side section structure;

[0017] Figure 3 This utility model Figure 1 A schematic diagram of the internal structure of the lower casing;

[0018] Figure 4 This utility model Figure 1 A schematic diagram of the bottom structure of the upper casing;

[0019] Figure 5 This utility model Figure 2 Enlarged structural diagram at point A in the middle.

[0020] In the diagram: 1. Upper clamping shell; 101. Clamping interface one; 102. Clamping interface two; 2. Lower clamping shell; 201. Clamping post one; 202. Clamping post two; 203. Adhesive shell one; 204. Abutting protrusion one; 205. Sample pad; 206. Sample absorption auxiliary strip; 207. Gold label pad; 208. NC membrane; 209. Detection line T; 210. Quality control line C; 211. Absorption pad; 212. Adhesive shell two; 213. Abutting protrusion two; 3. Observation groove; 4. Sample drop hole; 401. Spherical groove one; 402. Columnar groove two; 403. Spherical groove two; 404. Square guide groove; 405. Arc-shaped guide groove. Detailed Implementation

[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0022] Please see Figure 1-5A test card for detecting ferulic acid content in traditional Chinese medicine includes an upper casing 1 and a lower casing 2. The upper casing 1 has a sample drop inlet 4, which includes a spherical groove 401, a cylindrical groove 402, a spherical groove 403, a square guide groove 404, and an arc-shaped guide groove 405. The bottom of the spherical groove 401 has the cylindrical groove 402, and the bottom of the cylindrical groove 402 has the spherical groove 403. The spherical groove 401, cylindrical groove 402, and spherical groove 403 are connected. The inner wall of the cylindrical groove 402 has a square guide groove 404, and the inner wall of the spherical groove 403 has an arc-shaped guide groove 405. The device includes an arc-shaped flow channel 405, a square flow channel 404, and an arc-shaped flow channel 405 connected together. A sample absorption auxiliary strip 206 is engaged inside the arc-shaped flow channel 405. A sample pad 205 is fixedly connected to the bottom of the sample absorption auxiliary strip 206. A gold-labeled pad 207 is fixedly connected to the side of the sample pad 205. The gold-labeled pad 207 is coated with colloidal gold particles adsorbed with anti-ferulic acid monoclonal antibodies, or latex microspheres or fluorescent microspheres chemically bonded with anti-ferulic acid monoclonal antibodies. An NC membrane 208 is fixedly installed on the side of the gold-labeled pad 207, and an absorption pad 211 is fixedly installed on the side of the NC membrane 208. The middle section of panel 208 is equipped with a detection line T209 coated with ferulic acid antigen and a control line C210 coated with goat anti-mouse secondary antibody. A waist-shaped dropper hole is formed by spherical groove 1 (401), cylindrical groove 2 (402), and spherical groove 2 (403). During the dropper's inflow, the sample solution accumulates in the lower hemisphere of spherical groove 1 (401). The accumulated sample solution then enters spherical groove 2 (403) through cylindrical groove 2 (402). The upper hemisphere of spherical groove 2 (403) expands the dropper's surface area, ensuring sufficient sample solution volume. Simultaneously, the internal structures of cylindrical groove 2 (402) and spherical groove 2 (403) further enhance the dropper's inflow area. The interconnected square guide channel 404 forms an auxiliary liquid guiding operation. The introduced liquid then flows into the interconnected arc-shaped guide channel 405. The sample absorption auxiliary strip 206, which is engaged inside the arc-shaped guide channel 405, is used for absorption. The sample absorbs the sample and is then precipitated onto the sample pad 205 through the sample absorption auxiliary strip 206. This allows for the absorption of excess sample liquid. The ferulic acid in the sample liquid competes with the ferulic acid antigen on the detection line T209 to bind to the gold-labeled antibody. When the ferulic acid content in the sample liquid is high, more gold-labeled antibodies bind, and the amount of gold-labeled antibodies binding to the detection line T209 is less, resulting in a lighter color development of the detection line T209.When the ferulic acid content in the sample solution is low, fewer gold-labeled antibodies bind, resulting in a higher amount of gold-labeled antibody binding to the detection line T209, which shows a deeper color. The goat anti-mouse control line C210 can bind to all gold-labeled antibodies. The ferulic acid content level is determined by directly comparing the colors of the control line C210 and the detection line T209. A deeper color in the detection line T209 indicates a lower concentration of the target analyte in the sample solution. The quantitative detection card uses a reader to read the colors of the detection line T209 and the control line C210; different values ​​represent different contents. This detection card can also detect the ferulic acid content in samples such as traditional Chinese medicine, medicinal herbs, health products, and cosmetics for quantitative or qualitative analysis.

[0023] After completing the colorimetric operation described above, place the test card on an incubator. Use a pipette to draw 120 μL of the sample solution containing ferulic acid and add it vertically into the sample well of the test card. Cover the incubator and incubate for 10 minutes. Immediately after incubation, remove the test card and insert it into the liquid inlet of the reader, pointing outwards. Click "Start Test" to read the result.

[0024] Please see Figure 2 , Figure 3 and Figure 4 The upper casing 1 has an observation groove 3 located at the center. At the four corners of the lower surface of the upper casing 1, there are four locking interfaces 101. A locking post 201 is installed inside each locking interface 101. A lower casing 2 is fixedly installed at the bottom of the locking post 201. The bottom of the upper casing 1 has two locking interfaces 102, each containing a locking post 202. A fitting shell 203 is fixedly installed at the bottom of the lower casing 2. A sample pad 205 is fitted inside the cavity of the fitting shell 203. A sample pad 205 is fixedly installed inside the fitting shell 203. There are two abutting protrusions 204, the inner sides of which abut against the outer side of the sample pad 205. Two fitting shells 212 are fixedly installed at the bottom inside the lower housing 2. Abutting protrusions 213 are fixedly installed inside each of the two fitting shells 212. The inner sides of the abutting protrusions 213 abut against the outer side of the absorbent pad 211. The upper housing 1 and the lower housing 2 cooperate to form a hollow cuboid. The length and width of the upper housing 1 and the lower housing 2 are the same. The gold label pad 207 is sprayed with colloidal gold particles adsorbed with anti-ferulate monoclonal antibodies or latex microspheres (or fluorescent microspheres) chemically bonded with anti-ferulate monoclonal antibodies.

[0025] The working principle involves forming a waist-shaped droplet orifice through spherical groove 401, cylindrical groove 402, and spherical groove 403. During the droplet process, the sample liquid accumulates in the lower hemisphere of spherical groove 401. The accumulated sample liquid then enters spherical groove 403 through cylindrical groove 402. The upper hemisphere of spherical groove 403 expands the droplet area, ensuring sufficient sample liquid volume. Simultaneously, a square guide channel 404 connecting cylindrical groove 402 and spherical groove 403 provides auxiliary liquid guidance. The introduced liquid then flows into a connected arc-shaped guide channel 405, where it is absorbed by a sample absorption auxiliary strip 206. The sample absorbent then precipitates onto the sample pad 205 via the sample absorption auxiliary strip 206, thus absorbing excess volume. The sample solution is then tested. The ferulic acid in the sample solution competes with the ferulic acid antigen on the test line T209 for binding to the gold-labeled antibody. When the ferulic acid content in the sample solution is high, more gold-labeled antibodies bind, resulting in less binding to the test line T209 and a lighter color. Conversely, when the ferulic acid content is low, fewer gold-labeled antibodies bind, resulting in more binding to the test line T209 and a darker color. The goat anti-mouse antibody on the control line C210 can bind to all gold-labeled antibodies. The ferulic acid content level is determined by directly comparing the colors of the control line C210 and the test line T209. A darker test line T209 indicates a lower concentration of the target analyte in the sample solution. The quantitative detection card can be read numerically using a reader to measure the colors of the test line T209 and the control line C210; different values ​​represent different concentrations.

[0026] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0027] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A test card for detecting the ferulic acid content in traditional Chinese medicinal materials, characterized in that, The device includes an upper retaining shell (1) and a lower retaining shell (2). The upper retaining shell (1) has a sample dripping hole (4). The sample dripping hole (4) includes a spherical groove one (401), a cylindrical groove two (402), a spherical groove two (403), a square guide groove (404), and an arc guide groove (405). The bottom of the spherical groove one (401) has a cylindrical groove two (402), and the bottom of the cylindrical groove two (402) has a spherical groove two (403). The spherical groove one (401), the cylindrical groove two (402), and the spherical groove two (403) are connected. The inner wall of the cylindrical groove two (402) has a square guide groove (404), and the inner wall of the spherical groove two (403) has an arc guide groove (405). The square guide channel (404) and the arc-shaped guide channel (405) are connected. The arc-shaped guide channel (405) is fitted with a sample absorption auxiliary strip (206). The bottom of the sample absorption auxiliary strip (206) is fixedly connected to a sample pad (205). The side of the sample pad (205) is fixedly connected to a gold label pad (207). The side of the gold label pad (207) is fixedly installed with an NC membrane (208). The side of the NC membrane (208) is fixedly installed with an absorption pad (211). The middle part of the NC membrane (208) is respectively provided with a detection line T (209) coated with ferulic acid antigen and a quality control line C (210) coated with goat anti-mouse secondary antibody.

2. The detection card for detecting ferulic acid content in traditional Chinese medicinal materials according to claim 1, characterized in that: The upper casing (1) has card interfaces (101) at the four corners of its lower surface. Card interfaces (101) have card posts (201) inside them. The lower casing (2) is fixedly installed at the bottom of card posts (201).

3. The detection card for detecting ferulic acid content in traditional Chinese medicinal materials according to claim 1, characterized in that: The bottom of the upper casing (1) has two card interfaces (102), and the two card interfaces (102) are provided with card receiving posts (202).

4. The detection card for detecting ferulic acid content in traditional Chinese medicinal materials according to claim 1, characterized in that: The bottom of the lower housing (2) is fixedly installed with a first fitting shell (203). The sample pad (205) is attached inside the cavity of the first fitting shell (203). Two abutting protrusions (204) are fixedly installed inside the first fitting shell (203). The inner sides of the two abutting protrusions (204) abut against the outer side of the sample pad (205).

5. A detection card for detecting ferulic acid content in traditional Chinese medicinal materials according to claim 1, characterized in that: The bottom of the lower housing (2) is fixedly installed with two fitting shells (212). The two fitting shells (212) are fixedly installed with abutting protrusions (213) inside. The inner side of the abutting protrusions (213) abuts against the outer side of the absorbent pad (211).

6. A detection card for detecting ferulic acid content in traditional Chinese medicinal materials according to claim 1, characterized in that: The upper casing (1) has an observation slot (3) located at the center.

7. A detection card for detecting ferulic acid content in traditional Chinese medicinal materials according to claim 1, characterized in that: The upper casing (1) and the lower casing (2) cooperate to form a hollow cuboid. The length and width of the upper casing (1) and the lower casing (2) are the same. The gold pad (207) is sprayed with colloidal gold particles adsorbed with anti-ferulic acid monoclonal antibodies or latex microspheres chemically bonded with anti-ferulic acid monoclonal antibodies.

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