A device for the separation and purification of bisabolol

Abstract

The application discloses a boron carbide separation and purification device, in particular to the field of chromatographic separation, comprising a column body, characterized in that a plurality of mutually parallel and horizontal partitions are fixedly installed in the inner cavity of the column body, and the partitions are staggered in the inner cavity of the column body to form a reciprocating and zigzag flow channel from top to bottom; a sand core is arranged below the partitions to completely block the outlet of the inner cavity of the column body; two mutually parallel aperture plates are arranged above the partitions; a cover plate is installed at the lateral opening of the column body; the cover plate is provided with a ring-shaped protrusion matched with the lateral opening of the column body, and the column body is provided with a ring-shaped groove matched with the ring-shaped protrusion; the cover plate extends a first pressing plate matched with all the partitions; and the cover plate extends a second pressing plate inserted between the two aperture plates. The application prolongs the flow distance of the components to be separated in the stationary phase filler in the column body of limited length, the spacing between the components is enlarged with the increase of the flow distance, and the separation effect of the components is improved.

Description

Technical Field

[0001] This utility model belongs to the field of chromatographic separation technology, specifically relating to a bisabolol separation and purification device. Background Technology

[0002] Bisabolol is a precious natural ingredient with anti-inflammatory, antibacterial, repairing, and soothing skin properties, widely used in high-end cosmetics and pharmaceuticals. It is primarily derived from natural plants (such as German chamomile and myrrh resin) or obtained through chemical synthesis / biofermentation. Isolating and purifying bisabolol from natural sources is a multi-step, technically demanding process aimed at obtaining a high-purity product that meets cosmetic / pharmaceutical standards.

[0003] Column chromatography is commonly used in the separation and purification of bisabolol. However, when the physical and chemical properties of the components to be separated in the bisabolol stock solution are similar, it is difficult to achieve good separation results using a conventional length column, and the purity of the separated product is often insufficient. Therefore, a novel bisabolol separation and purification device is needed. Utility Model Content

[0004] To address the aforementioned problems, this utility model discloses a bisabolol separation and purification device.

[0005] To achieve the above objectives, the technical solution of this utility model is as follows:

[0006] A bisabolol separation and purification device includes a column, characterized in that a plurality of parallel and horizontal partitions are fixedly installed in the inner cavity of the column, and the partitions are staggered in the inner cavity of the column to form a reciprocating and tortuous flow channel from top to bottom; a sand core is provided below the partitions to completely block the outlet of the inner cavity of the column; two parallel perforated plates are provided above the partitions; a cover plate is installed at the lateral opening of the column; the cover plate is provided with an annular protrusion adapted to the lateral opening of the column; and the column is provided with an annular groove adapted to the annular protrusion; the cover plate extends to a first pressing plate adapted to the insertion of all the partitions; and the cover plate extends to a second pressing plate inserted between the two perforated plates.

[0007] As a preferred embodiment of this utility model, the edge of the cover plate is evenly permeated with a number of mounting bolts, and each mounting bolt is threadedly connected to the column.

[0008] As a preferred technical solution of this utility model, the top feed port of the column is threadedly connected to a top cover, the top cover is fixedly connected to at least two feed pipes that penetrate through itself and extend into the inner cavity of the column, and the top cover is fixedly connected to a vent valve that penetrates through itself and extends into the inner cavity of the column.

[0009] As a preferred embodiment of this utility model, the bottom of the feed pipe is provided with several side openings, and the feed pipe extends laterally to form a barrier that covers all the side openings, and the barrier runs through the entire structure.

[0010] As a preferred technical solution of this utility model, the venting valve includes a valve body fixedly connected to the top cover, a valve plug is threadedly connected to the top annular groove of the valve body, and the conical plug of the inner cavity of the valve plug is vertically inserted into the top opening of the valve body. The top of the valve plug is provided with several through holes that communicate laterally with the outside.

[0011] As a preferred embodiment of this utility model, at least one side wall of the column without a lateral opening is provided with a vertical observation window.

[0012] As a preferred embodiment of this utility model, the length of the observation window at least covers the distribution area of ​​the partition and the perforated plate.

[0013] As a preferred embodiment of this utility model, the area of ​​the partition covers more than 70% of the cross-section of the inner cavity of the column.

[0014] The beneficial effects of this utility model are as follows:

[0015] 1. The column cavity of this application is provided with a reciprocating tortuous flow channel, which lengthens the flow distance of the components to be separated in the stationary phase packing within the finite length of the column. The spacing between each component increases with the increase of the flow distance, thereby improving the separation effect of each component in the bisabolol stock solution. At the same time, the column cavity of this application can be repeatedly filled with stationary phase packing and degreased cotton to meet the needs of multiple uses.

[0016] 2. The feed pipe outlet of this application is equipped with a side opening and a baffle, which can prevent the input solvent from directly impacting the components to be separated and affecting the separation results, thus ensuring the accuracy of the separation;

[0017] Third, the vent valve of this application is a spiral opening and closing valve, which can be freely adjusted in terms of opening degree. Moreover, the movement stroke of the valve plug is restricted by the annular groove of the valve body, making it difficult for the valve plug to accidentally fly off when it is opened under certain pressure. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of an embodiment of the present utility model;

[0019] Figure 2 This is an exploded view from another angle of an embodiment of the present invention;

[0020] Figure 3 This is a schematic diagram of the structure of this utility model with the cover plate removed.

[0021] Figure 4This is a schematic diagram of the feed pipe structure according to an embodiment of the present utility model;

[0022] Figure 5 This is a schematic diagram of the structure of the vent valve in an embodiment of this utility model.

[0023] List of identifiers in attached diagrams:

[0024] 1. Column; 2. Partition; 3. Cover plate; 4. First pressing plate; 5. Second pressing plate; 6. Sand core; 7. Perforated plate; 8. Top cover;

[0025] 9. Vent valve; 901. Valve body; 902. Valve plug;

[0026] 10. Feed pipe; 11. Mounting bolts. Detailed Implementation

[0027] The present invention will be further explained below with reference to the accompanying drawings and specific embodiments. It should be understood that the following specific embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0028] Please see Figure 1-5 A bisabolol separation and purification device includes a column 1. Several parallel and horizontal baffles 2 are fixedly installed inside the column 1, and the baffles 2 are staggered to form a reciprocating and tortuous flow channel from top to bottom within the column 1. The area of ​​the baffles 2 covers more than 70% of the cross-section of the column 1's inner cavity, ensuring that the components to be separated flow along the flow channel in a reciprocating and tortuous manner, fully facilitating the stationary phase packing. Below the baffles 2, a sand core 6 is installed to completely block the outlet of the column 1's inner cavity, and the sand core 6 can completely filter the powdered stationary phase packing. Above the baffles 2, two parallel perforated plates 7 are installed, and the space between the two perforated plates 7 is used to plug degreased cotton. A cover plate 3 is installed at the lateral opening of the column 1. The cover plate 3 has an annular protrusion adapted to the lateral opening of the column 1, and the column 1 has an annular groove adapted to the annular protrusion. The annular protrusion surrounds the lateral opening of the column 1. The cover plate 3 extends to a first pressing plate 4 adapted to fit all the partitions 2, and the cover plate 3 also extends to a second pressing plate 5 inserted between the two orifice plates 7. A corrosion-resistant rubber seal is provided at the connection between the partitions 2, the first pressing plate 4, and the cover plate 3 to prevent solvent-eluted products from directly passing through the partitions 2 at the connection, thus preventing the components from being effectively separated by the stationary phase. Several mounting bolts 11 are evenly distributed along the edge of the cover plate 3, and each mounting bolt 11 is threaded to the column 1.

[0029] The top inlet of column 1 is threadedly connected to a top cover 8, which is fixedly connected to at least two feed pipes 10 that penetrate through itself and extend into the inner cavity of column 1. The top cover 8 is also fixedly connected to a vent valve 9 that penetrates through itself and extends into the inner cavity of column 1. The bottom outlet pipe of column 1 is equipped with a stopcock for discharging liquid.

[0030] The bottom of the feed pipe 10 has several side openings. The feed pipe 10 extends laterally to form a barrier that covers all the side openings and extends vertically through the pipe. The liquid at the bottom of the feed pipe 10 first flows laterally out of the side openings and then drips vertically. The side openings and the barrier of the feed pipe 10 can prevent the solution from directly washing over the components to be separated.

[0031] The vent valve 9 includes a valve body 901 fixedly connected to the top cover 8. A valve plug 902 is threaded into the annular groove at the top of the valve body 901, and the conical plug inside the valve plug 902 is vertically inserted into the top opening of the valve body 901. The top of the valve plug 902 has several through holes that communicate laterally with the outside. When the valve plug 902 is tightened, the conical plug inside the valve plug 902 blocks the top opening of the valve body 901. When the valve plug 902 is loosened, the conical plug inside the valve plug 902 disengages from the top opening of the valve body 901, and the inner cavity of the valve body 901 communicates with the outside through the through holes of the valve plug 902. The movement stroke of the valve plug 902 is limited by the annular groove of the valve body 901.

[0032] At least one sidewall of the column 1 without lateral openings has a vertical observation window. The length of the observation window at least covers the distribution area of ​​the partition 2 and the orifice plate 7. The observation window allows observation of the liquid level in the column 1, and if colored substances are being separated, the separation status can also be observed. Providing multiple observation windows increases the amount of light entering the cavity of the column 1, facilitating observation of the liquid level position.

[0033] Working principle:

[0034] When filling the stationary phase, remove the cover plate 3 and lay the column 1 flat. Fill the space between the two perforated plates 7 with degreased cotton. The partition plate 2 forms a reciprocating and downward flow channel in the inner cavity of the column 1, which is filled with stationary phase filler (such as silica gel powder). Then, cover the cover plate 3 and tighten the mounting bolt 11 through the cover plate 3 onto the column 1, so that the annular protrusion of the cover plate 3 is pressed into the annular groove of the column 1 to form a seal. At the same time, the first pressing plate 4 compacts the stationary phase filler, and the second pressing plate 5 squeezes the degreased cotton to fill the space between the two perforated plates 7.

[0035] In operation, the two feed pipes 10 are connected to the pipelines supplying the component to be separated (liquid) and the solvent, respectively. Then, the vent valve 9 is opened, and the stopcock of the column 1 is closed. One feed pipe 10 supplies solvent into the inner cavity of the column 1 to wet the stationary phase packing until the two orifice plates 7 are completely submerged (observed through the observation window of the column 1). Next, the stopcock is opened to release the solvent until the two orifice plates 7 are just completely submerged. The stopcock is closed again. Then, the other feed pipe 10 supplies the component to be separated into the inner cavity of the column 1. Then, the vent valve 9 is closed, and the stopcock is opened while the feed pipe 10 continuously and slowly supplies solvent into the inner cavity of the column 1. The solvent is slowly supplied (the supply pressure can be controlled according to the actual situation) to elute the components to be separated. The components to be separated move at different speeds in the stationary phase packing. The components flow out sequentially from the bottom of column 1 (using conventional segmented collection, the collected components are detected by thin plate chromatography, and finally the same components are combined). The reciprocating and tortuous flow channel in the limited length of column 1 lengthens the flow distance of the components to be separated in the stationary phase packing. The spacing between the components increases with the increase of the flow distance, which improves the separation effect of each component in the bisabolol stock solution.

[0036] It should be noted that the above content merely illustrates the technical concept of this utility model and cannot be used to limit the scope of protection of this utility model. For those skilled in the art, several improvements and modifications can be made without departing from the principle of this utility model, and all such improvements and modifications fall within the scope of protection of the claims of this utility model.

Claims

1. A bisabolol separation and purification device, comprising a column (1), characterized in that, The inner cavity of the column (1) is fixedly equipped with several parallel and horizontal partitions (2), and the partitions (2) are staggered in the inner cavity of the column (1) to form a reciprocating and tortuous flow channel from top to bottom. A sand core (6) is provided below the partition (2) to completely block the outlet of the inner cavity of the column (1). Two parallel perforated plates (7) are provided above the partition (2). A cover plate (3) is installed at the lateral opening of the column (1). The cover plate (3) is provided with an annular protrusion adapted to the lateral opening of the column (1), and the column (1) is provided with an annular groove adapted to the annular protrusion. The cover plate (3) extends with a first pressing plate (4) adapted to the insertion of all the partitions (2), and the cover plate (3) extends with a second pressing plate (5) inserted between the two perforated plates (7).

2. The bisabolol separation and purification device according to claim 1, characterized in that, The edge of the cover plate (3) is evenly perforated with several mounting bolts (11), and each mounting bolt (11) is threadedly connected to the column (1).

3. The bisabolol separation and purification device according to claim 1, characterized in that, The top feed port of the column (1) is threadedly connected to a top cover (8), and the top cover (8) is fixedly connected to at least two feed pipes (10) that penetrate through itself and extend into the inner cavity of the column (1), and the top cover (8) is fixedly connected to a vent valve (9) that penetrates through itself and extends into the inner cavity of the column (1).

4. The bisabolol separation and purification device according to claim 3, characterized in that, The bottom of the feed pipe (10) is provided with several side openings. The feed pipe (10) extends laterally to form a barrier that covers all the side openings and extends through the top and bottom.

5. The bisabolol separation and purification device according to claim 3, characterized in that, The venting valve (9) includes a valve body (901) fixedly connected to the top cover (8). A valve plug (902) is threadedly connected to the top annular groove of the valve body (901). The conical plug of the inner cavity of the valve plug (902) is vertically inserted into the top opening of the valve body (901). The top of the valve plug (902) is provided with several through holes that communicate laterally with the outside.

6. The bisabolol separation and purification apparatus according to claim 1, characterized in that, The column (1) has at least one side wall without a lateral opening that has a vertical observation window.

7. The bisabolol separation and purification apparatus according to claim 6, characterized in that, The length of the observation window covers at least the distribution area of ​​the partition (2) and the perforated plate (7).

8. The bisabolol separation and purification apparatus according to claim 1, characterized in that, The area of ​​the partition (2) covers more than 70% of the cross-section of the inner cavity of the column (1).

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