A rapid nucleic acid extraction and purification device

Abstract

The utility model discloses a kind of rapid nucleic acid extraction and purification device, including release bin, the elution bin in the release bin one side, the one side of release bin is connected with second rotary knob by screw thread rotation, the funnel bin that the one side of second rotary knob is fixed with is penetrated to release bin inside, the one side of second rotary knob and the one side of release bin are all provided with first rotary knob;The utility model is provided with bellows in the inside of first bottle cap, when connecting first bottle cap with silica gel filter membrane with elution bin, squeeze elution bin at this time, nucleic acid eluent in the inside of elution bin is entered the inside of first bottle cap through silica gel filter membrane, the bellows of setting stabilizes the internal pressure of first bottle cap, nucleic acid eluent and silica gel filter membrane cooperate to elute the nucleic acid of silica gel filter membrane down at this time, after reverse squeeze bellows, eluent in the inside of first bottle cap is pushed to the inside of elution bin again, reciprocating repeat above-mentioned step, improve the mixing effect of silica gel filter membrane and eluent.

Description

Technical Field

[0001] This utility model relates to the field of nucleic acid extraction, specifically to a rapid nucleic acid extraction and purification device. Background Technology

[0002] Nucleic acid extraction and purification is the process of extracting nucleic acids from biological samples, purifying them, and removing impurities and contaminants. As a basic pretreatment step in molecular diagnostics, the efficiency and quality of nucleic acid extraction directly affect the accuracy and sensitivity of subsequent nucleic acid detection.

[0003] A rapid nucleic acid extraction and purification device, patent number CN219930051U, is described. In use, a partition plate is installed inside the extraction and purification bottle, dividing it into a release chamber and an elution chamber. A first cap and a second cap are threaded to both ends of the extraction and purification bottle, respectively. A bottle mouth cap is threaded to the top of the first cap. A silica gel filter membrane is fixed inside the first cap. A nucleic acid releasing agent is placed in the release chamber, and a nucleic acid eluent is placed in the elution chamber. After sample collection, a flocked swab is placed in the release chamber, where the nucleic acid releasing agent reacts with the sample. The bottle mouth cap is then opened, and the liquid is squeezed out. The released nucleic acid is adsorbed onto the silica gel filter membrane of the first cap. The second cap is then removed and interchanged with the first cap. The extraction and purification bottle is then shaken to allow the nucleic acid eluent to contact the silica gel filter membrane of the first cap, thereby eluting the nucleic acid from the silica gel filter membrane and completing the nucleic acid extraction and purification. The device has an overall tubular structure, is small in size, easy to carry, and has few limitations.

[0004] However, current rapid nucleic acid extraction and purification devices have poor mixing effects between silica membranes and eluents, resulting in low nucleic acid elution efficiency. They are also cumbersome to operate, have a high risk of contamination, and lack an active driving mechanism. Utility Model Content

[0005] To address the aforementioned technical problems, a rapid nucleic acid extraction and purification device is provided. This technical solution overcomes the shortcomings mentioned in the background technology: low elution efficiency, high risk of contamination, and lack of active drive.

[0006] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0007] A rapid nucleic acid extraction and purification device includes a release chamber and an elution chamber located on one side of the release chamber. A second rotary knob is rotatably connected to one side of the release chamber via a thread. A funnel chamber penetrating into the interior of the release chamber is fixed to one side of the second rotary knob. A first rotary knob is provided on one side of the second rotary knob and the opposite side of the release chamber. The two first rotary knobs are respectively connected to a first bottle cap and a second bottle cap. Corrugated tubes are integrated inside the first bottle cap and the second bottle cap. The first rotary knob is connected to an externally threaded tube. The port of the elution chamber and the inner wall of the second rotary knob are provided with internal threads that mate with the externally threaded tube.

[0008] Preferably, the first bottle cap and the second bottle cap have a liquid outlet on one side, and the inside of the liquid outlet is connected to a plug by a threaded connection.

[0009] Preferably, an external threaded tube is fixed to one side of the first rotary knob, and the inner wall of the washing chamber and the inner wall of the second rotary knob are provided with internal threads that cooperate with the external threaded tube.

[0010] Preferably, a silicone filter membrane is disposed inside one of the externally threaded tubes, and the corrugated tube is made of medical-grade silicone with a thickness of ± mm and a number of corrugations of - layers.

[0011] Preferably, the release chamber contains a nucleic acid releasing agent, and the elution chamber contains a nucleic acid elution solution.

[0012] Preferably, the release chamber and the washing chamber are both made of plastic material and are integrally molded, and the release chamber and the washing chamber are not connected.

[0013] Preferably, a locking opening is provided on one side of the funnel chamber, and a through hole is provided at one end of the outer side of the funnel chamber.

[0014] Preferably, the bellows has a Shore hardness of 40A±5, a resilience of ≥0.6N, a stroke of 10±1mm, and a maximum volume change of 1.8mL.

[0015] Preferably, the mating depth between the external threaded pipe and the internal thread is 8±0.5mm, and the tightening torque is 0.6-0.8N·m.

[0016] Compared with the prior art, this utility model provides a rapid nucleic acid extraction and purification device, which has the following beneficial effects:

[0017] (1) Elution efficiency is significantly improved

[0018] By setting a medical silicone corrugated tube (7) (thickness 1.5±0.2mm, number of corrugations 8-10 layers, Shore hardness 40A±5) inside the first cap (3): when the elution chamber (1) is squeezed, a positive pressure of 0.15-0.3MPa is generated, driving the eluent to penetrate the micropores of the silicone filter membrane (9) at a high speed of 0.8m / s, and the pore contact rate is greatly improved; when the pressure is released, the corrugated tube rebounds, and the eluent is drawn back to peel off the deep nucleic acid; the cycle is repeated 5-8 times, which improves the nucleic acid recovery rate.

[0019] (2) Pollution risks have been completely eliminated

[0020] The design adopts a threaded quick-connect design (the external threaded tube (15) and the internal thread (13) of the washing chamber (1) are directly matched): thread depth 8±0.5mm + tightening torque 0.6-0.8N·m → axial locking force 75-100N, to achieve biosafety level sealing.

[0021] (3) Efficient and simplified operation process

[0022] The corrugated tube self-circulation control system replaces manual shaking, shortening the elution time; the pre-filled reagents (nucleic acid release agent in the release chamber + nucleic acid elution buffer in the elution chamber) further reduce the number of operation steps. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0024] Figure 2 This is the overall structure of the present utility model;

[0025] Figure 3 This is a schematic diagram of the connection of the funnel chamber of this utility model;

[0026] Figure 4 This is a schematic diagram of the internal structure of the first bottle cap of this utility model.

[0027] The numbers on the map are:

[0028] 1. Washing chamber; 2. Release chamber; 3. First cap; 4. Second cap; 5. First rotary knob; 6. Second rotary knob; 7. Corrugated tube; 8. Plug; 9. Silica gel filter membrane; 10. Funnel chamber; 11. Clamping port; 12. Through hole; 13. Internal thread; 14. Liquid outlet; 15. External threaded tube. Detailed Implementation

[0029] The following description is intended to disclose the present invention so that those skilled in the art can implement it. The preferred embodiments described below are merely examples, and other obvious variations will occur to those skilled in the art.

[0030] Example 1

[0031] Please refer to Figures 1-4 As shown, a rapid nucleic acid extraction and purification device includes a release chamber 2, an elution chamber 1 located on one side of the release chamber 2, a second rotary knob 6 connected to one side of the release chamber 2 by a thread, a funnel chamber 10 fixed to one side of the second rotary knob 6 extending into the interior of the release chamber 2, a first rotary knob 5 on one side of the second rotary knob 6 and the opposite side of the release chamber 2, the two first rotary knobs 5 being respectively connected to a first cap 3 and a second cap 4, a corrugated tube 7 integrated inside the first cap 3 and the second cap 4, the first rotary knob 5 being connected to an externally threaded tube 15, the port of the elution chamber 1 and the inner wall of the second rotary knob 6 being provided with an internal thread 13 that mates with the externally threaded tube 15, a silicone filter membrane 9 being disposed inside one of the externally threaded tubes 15, and the corrugated tube 7 being made of medical-grade silicone with a thickness of 1.5±0.2mm and 8-10 corrugations;

[0032] In this embodiment, by interchanged first cap 3 and second cap 4, the liquid inside release chamber 2 comes into contact with the silica gel filter membrane 9 through funnel chamber 10. The nucleic acid releasing agent inside release chamber 2 comes into contact with the swab head, dissolving the nucleic acid on the swab head. Then, first cap 3 is connected to release chamber 2, and then plug 8 is rotated to squeeze the dissolved nucleic acid release solution out of outlet 14. At the same time, the nucleic acid release solution comes into contact with silica gel filter membrane 9, and the nucleic acid adheres to silica gel filter membrane 9. Then, first cap 3 and second cap 4 are interchanged. Then, elution chamber 1 is squeezed so that the nucleic acid eluent inside elution chamber 1 flows from one side of silica gel filter membrane 9 to the other side, so that the nucleic acid detaches from silica gel filter membrane 9 and mixes with the nucleic acid eluent. Then, corrugated tube 7 is squeezed again so that the nucleic acid eluent that entered the first cap 3 re-enters the interior of elution chamber 1. The above steps are repeated to elute the nucleic acid from silica gel filter membrane 9, completing the extraction and purification of nucleic acid.

[0033] Example 2

[0034] Please refer to Figure 3 and Figure 4 As shown, the first bottle cap 3 and the second bottle cap 4 have liquid outlets 14 on one side. The inside of the liquid outlet 14 is connected to a plug 8 by a threaded rotation. The first rotary knob 5 is fixed with an external thread tube 15 on one side. The inner wall of the washing chamber 1 and the inner wall of the second rotary knob 6 are provided with internal threads 13 that cooperate with the external thread tube 15.

[0035] In this embodiment, the outlet 14 facilitates the flow of nucleic acid release agent out of the release chamber 2. At the same time, the corrugated tube 7 facilitates the reciprocating compression of the nucleic acid release agent, so that the silica gel filter membrane 9 and the nucleic acid contained in the nucleic acid release agent are fully adsorbed. The plug 8 facilitates the opening and closing of the outlet 14, thereby facilitating the flow of nucleic acid release agent. The internal thread 13 and the external thread tube 15 cooperate with each other to facilitate the interchange of the first cap 3 and the second cap 4.

[0036] Example 3

[0037] Please refer to Figure 1 As shown, nucleic acid releasing agent is added inside the release chamber 2, and nucleic acid eluent is added inside the elution chamber 1. Both the release chamber 2 and the elution chamber 1 are made of plastic material and are integrally molded. The release chamber 2 and the elution chamber 1 are not connected.

[0038] In this embodiment, the nucleic acid on the swab head is dissolved in the nucleic acid releasing agent by the set nucleic acid releasing agent, and the nucleic acid elution solution is set so that the nucleic acid on the silica gel filter membrane 9 is dissolved in the nucleic acid elution solution. The release chamber 2 and the elution chamber 1 made of plastic material are easy to squeeze to drive the flow of nucleic acid releasing agent and nucleic acid elution solution.

[0039] Example 4

[0040] Please refer to Figure 3 As shown, a clamping port 11 is provided on one side of the funnel chamber 10, and a through hole 12 is provided at one end of the outer side of the funnel chamber 10. The Shore hardness of the bellows 7 is 40A±5, the rebound force is ≥0.6N, the stroke of the bellows 7 is 10±1mm, which can facilitate the rapid rebound of the bellows 7 and realize the self-circulation of the bellows. The maximum volume change is 1.8mL. The mating depth between the external thread tube 15 and the internal thread 13 is 8±0.5mm, and the tightening torque is 0.6-0.8N·m.

[0041] In this embodiment, the swab head with nucleic acid is inserted into the clamping port 11, and then the swab head is rotated to one side. The end of the swab head fits into the clamping port 11, thereby causing the swab head to break, which facilitates the installation of the first bottle cap 3. The threaded quick-connect design (the external threaded tube (15) and the internal thread (13) of the washing chamber (1) directly cooperate): thread depth 8±0.5mm + tightening torque 0.6-0.8N·m → axial locking force 75-100N, to achieve biosafety level sealing.

[0042] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A rapid nucleic acid extraction and purification device, comprising a release chamber (2) and an elution chamber (1) located on one side of the release chamber (2), characterized in that: A second rotary knob (6) is connected to one side of the release chamber (2) by a threaded rotation. A funnel chamber (10) is fixed to one side of the second rotary knob (6) and extends into the interior of the release chamber (2). A first rotary knob (5) is provided on one side of the second rotary knob (6) and the opposite side of the release chamber (2). The two first rotary knobs (5) are respectively connected to the first bottle cap (3) and the second bottle cap (4). A corrugated tube (7) is integrated inside the first bottle cap (3) and the second bottle cap (4). The first rotary knob (5) is connected to an external threaded tube (15). The port of the washing chamber (1) and the inner wall of the second rotary knob (6) are provided with an internal thread (13) that mates with the external threaded tube (15).

2. The rapid nucleic acid extraction and purification device according to claim 1, characterized in that: The first bottle cap (3) and the second bottle cap (4) have a liquid outlet (14) on one side, and the inside of the liquid outlet (14) is connected to a plug (8) by a threaded rotation.

3. The rapid nucleic acid extraction and purification device according to claim 1, characterized in that: One side of the first rotary knob (5) is fixed with an external threaded tube (15), and the inner wall of the washing chamber (1) and the inner wall of the second rotary knob (6) are provided with an internal thread (13) that cooperates with the external threaded tube (15).

4. The rapid nucleic acid extraction and purification device according to claim 3, characterized in that: A silicone filter membrane (9) is provided inside one of the externally threaded tubes (15), and the corrugated tube (7) is made of medical-grade silicone with a thickness of 1.5±0.2mm and 8-10 layers of corrugations.

5. The rapid nucleic acid extraction and purification device according to claim 1, characterized in that: The release chamber (2) contains a nucleic acid release agent, and the elution chamber (1) contains a nucleic acid elution solution.

6. The rapid nucleic acid extraction and purification device according to claim 1, characterized in that: The release chamber (2) and the washing chamber (1) are both made of plastic material and are integrally formed. The release chamber (2) and the washing chamber (1) are not connected.

7. The rapid nucleic acid extraction and purification device according to claim 1, characterized in that: A locking opening (11) is provided on one side of the funnel chamber (10), and a through hole (12) is provided at one end of the outer side of the funnel chamber (10).

8. The apparatus according to claim 2, characterized in that: The bellows (7) has a Shore hardness of 40A±5, a resilience of ≥0.6N, a stroke of 10±1mm, and a maximum volume change of 1.8mL.

9. The apparatus according to claim 1, characterized in that: The mating depth between the external threaded pipe (15) and the internal thread (13) is 8±0.5mm, and the tightening torque is 0.6-0.8N·m.

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