Microbial inoculant cultivation and propagation device
Patent Information
- Application Number
- CN202522169348.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-10-14
- Publication Date
- 2026-09-08
- Estimated Expiration
- 2035-10-14
AI Technical Summary
[0003]壁面菌液会因脱离主体培养体系,无法及时获取营养、氧气,或因罐壁温度与体系温度存在差异(如加热 、 冷却时),导致菌群生长缓慢、活性下降,甚至出现局部代谢异常;微生物菌剂培育的核心目标是获得足量、高活性的成品菌剂,粘附于罐壁的菌液若不回收,会直接造成有效产物损失;
[0016] This invention provides a microbial inoculant cultivation and propagation device, which has the following beneficial effects:
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Figure CN224728526U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of microbial agent propagation technology, specifically a microbial agent cultivation and propagation device. Background Technology
[0002] Microbial inoculant cultivation and propagation is the core process of gradually expanding the cultivation scale and increasing the number and activity of viable bacteria of "functional strains" screened in the laboratory through standardized processes, ultimately achieving industrial-scale mass production. Its core objective is to create an optimal growth environment for microorganisms while controlling contamination, ensuring that the viable bacteria count, stability, and functional effectiveness of the final product meet standards.
[0003] The bacterial solution adhering to the tank wall may become detached from the main culture system and unable to obtain nutrients and oxygen in time. Alternatively, the temperature difference between the tank wall and the system (such as during heating or cooling) may cause the bacterial community to grow slowly, its activity to decrease, or even cause local metabolic abnormalities. The core objective of microbial agent cultivation is to obtain a sufficient quantity of highly active finished agent. If the bacterial solution adhering to the tank wall is not recovered, it will directly cause the loss of effective products.
[0004] The utility model patent with patent authorization announcement number CN 219279863 U discloses a microbial agent cultivation and propagation device. The specification describes a method in which a rotating scraper scrapes off the bacterial liquid adhering to the inner wall of the propagation unit, allowing the bacterial liquid to fall into the discharge pipe and improving the discharge effect. However, the scraper is always in contact with the inner wall of the tank. Essentially, the two surfaces generate "sliding friction" in relative motion (such as the scraper rotating with the stirring shaft). This friction directly leads to the wear and tear of both materials. Moreover, the continuous friction of the scraper will gradually damage the smoothness of the inner wall, forming fine scratches and pits on the wall surface. These rough surfaces will become "new adhesion points" for the bacterial liquid, which will aggravate the bacterial liquid residue in the subsequent cultivation process. Utility Model Content
[0005] (a) Technical problems to be solved
[0006] To address the shortcomings of existing technologies, this invention provides a microbial agent cultivation and propagation device, which solves the problems mentioned in the background section.
[0007] (II) Technical Solution
[0008] To achieve the above objectives, this utility model is implemented through the following technical solution: a microbial agent cultivation and propagation device, comprising a tank, a vertically arranged main pipe rotatably inserted into the top of the tank, the lower end of the main pipe being located inside the tank, and a plurality of hollow stirring rods being connected to the lower end of the main pipe, a slider being slidably arranged inside the stirring rod, and an insert rod being fixedly connected to the other end of the slider, the other ends of the plurality of insert rods extending to the outside of the stirring rod and being fixedly connected to a scraper, a spring being fixedly connected between the slider and the inner end of the stirring rod, the spring being sleeved on the insert rod, and the scraper being able to abut against the inner wall of the tank when pressed;
[0009] A bracket is fixedly connected to the top of the tank, and an air inlet pipe is fixedly installed on the bracket. The other end of the air inlet pipe is inserted into the top of the main pipe, and a three-way valve is fixedly installed on the other end of the air inlet pipe.
[0010] Preferably, a motor is fixedly installed on the top of the tank, a drive gear is fixedly connected to the output end of the motor, and a driven gear is fixedly sleeved on the upper end of the main pipe, with the drive gear and the driven gear meshing in contact.
[0011] Preferably, the scraper has an arc-shaped structure on the side closest to the inner wall of the tank, and the scraper is made of silicone rubber.
[0012] Preferably, the other port of the three-way valve is fixedly connected to an air inlet pipe two, and the bottom of the tank body is fixedly connected to an annular exhaust pipe. Multiple exhaust nozzles are connected to the annular exhaust pipe, and the other end of the air inlet pipe two is connected to the annular exhaust pipe.
[0013] Preferably, a discharge pipe is fixedly installed at the bottom of the tank body, and a valve is provided on the discharge pipe.
[0014] Preferably, a feed pipe is fixedly connected to the top of the tank, and the feed pipe is configured to communicate with the inside of the tank.
[0015] (III) Beneficial Effects
[0016] This invention provides a microbial inoculant cultivation and propagation device, which has the following beneficial effects:
[0017] 1. In this utility model, during the stirring process, the scraper does not come into contact with the inner wall of the tank, and the stirring rod fully mixes the microbial agent inside the tank. When it is necessary to scrape off the microbial agent from the inner wall of the tank, the three-way valve is connected to an external oxygen supply device (such as an oxygen cylinder), and the generated oxygen is delivered to the main pipe through the air inlet pipe, and finally delivered to each stirring rod. Then, the high-pressure oxygen pushes the slider to move, so that the scraper comes into contact with the inner wall of the tank. At this time, the main pipe is driven to rotate, which drives the scraper to rotate and scrapes off the finished microbial agent from the inner wall of the tank. This method only makes the scraper come into contact with the inner wall of the tank when scraping is needed, reducing the wear between the scraper and the surface of the tank, and avoiding the formation of small scratches and pits on the tank wall as much as possible.
[0018] 2. In this utility model, the oxygen delivery can be redirected by setting a three-way valve. Therefore, the oxygen generated by the oxygen supply equipment can be discharged into the annular exhaust pipe through the second air inlet pipe and finally discharged through multiple exhaust nozzles, so as to supply oxygen to the bacterial solution prepared by the microbial strains, thereby flexibly realizing the switching between nutrient supply and pressurized scraping. Attached Figure Description
[0019] Figure 1 This is a front-view perspective view of a microbial agent cultivation and propagation device proposed in this utility model;
[0020] Figure 2 This is a front cross-sectional view of a microbial agent cultivation and propagation device proposed in this utility model;
[0021] Figure 3 for Figure 2 Enlarged structural diagram at point A;
[0022] Figure 4 This is a top cross-sectional view of a microbial agent cultivation and propagation device proposed in this utility model.
[0023] In the diagram: 1. Tank body; 2. Main pipe; 3. Stirring rod; 4. Insert rod; 5. Sliding block; 6. Scraper; 7. Feed pipe; 8. Motor; 9. Drive gear; 10. Air inlet pipe one; 11. Three-way valve; 12. Air inlet pipe two; 13. Annular exhaust pipe; 14. Exhaust nozzle; 15. Discharge pipe; 16. Support; 17. Spring; 18. Driven gear. Detailed Implementation
[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.
[0025] Please see Figures 1 to 4This utility model provides a technical solution: a microbial agent cultivation and propagation device, including a tank 1, a vertically arranged main pipe 2 rotatably inserted into the top of the tank 1, the lower end of the main pipe 2 being located inside the tank 1, and a plurality of hollow stirring rods 3 being connected to the lower end of the main pipe 2, a slider 5 being slidably arranged inside the stirring rod 3, and an insertion rod 4 being fixedly connected to the other end of the slider 5, the other ends of the plurality of insertion rods 4 extending to the outside of the stirring rod 3 and being fixedly connected to a scraper 6, a spring 17 being fixedly connected between the slider 5 and the inner end of the stirring rod 3, the spring 17 being sleeved on the insertion rod 4, and the scraper 6 being able to abut against the inner wall of the tank 1 when pressed;
[0026] During the mixing process, the scraper 6 does not contact the inner wall of the tank 1, and the rotating stirring rod 3 fully mixes the microbial agent inside the tank 1. When it is necessary to scrape off the microbial agent from the inner wall of the tank 1, the three-way valve 11 is connected to an external oxygen supply device (such as an oxygen cylinder), and the generated oxygen is delivered to the main pipe 2 through the air inlet pipe 10, and finally delivered to each stirring rod 3. Then, the high-pressure oxygen pushes the slider 5 to move, and the slider 5 pushes the insert rod 4 to move linearly, so that the scraper 6 contacts the inner wall of the tank 1. When the spring 17 is compressed, the main pipe 2 is driven to rotate, which in turn drives the scraper 6 to rotate and scrape off the finished bacterial agent on the inner wall of the tank 1. This method only involves the scraper 6 contacting the inner wall of the tank 1 when scraping is required, reducing the wear between the scraper 6 and the surface of the tank 1, and avoiding the formation of small scratches and pits on the surface of the tank 1 as much as possible. After scraping is completed, the air pressure generated by the oxygen supply equipment is removed, the spring 17 releases its elasticity, drives the scraper 6 to return to its original position, and the gas inside the stirring rod 3 and the main pipe 2 can be discharged through the three-way valve 11.
[0027] To determine whether the scraper 6 is in effective contact with the inner wall of the tank 1, setting an observation port on the surface of the tank 1 is the preferred method. The key is to directly observe the relative position of the scraper 6 and the inner wall through the transparent window.
[0028] See Figure 1 A bracket 16 is fixedly connected to the top of the tank body 1. An air inlet pipe 10 is fixedly installed on the bracket 16. The other end of the air inlet pipe 10 is inserted into the top of the main pipe 2. A three-way valve 11 is fixedly installed on the other end of the air inlet pipe 10.
[0029] The three ports of the three-way valve 11 are connected to the first air inlet pipe 10, the second air inlet pipe 12, and the oxygen supply equipment, respectively. The oxygen supply equipment can generate high-pressure oxygen to supply oxygen to the microbial agents inside the tank 1 and to push and squeeze the scraper 6.
[0030] See Figure 1 A motor 8 is fixedly installed on the top of the tank body 1. A drive gear 9 is fixedly connected to the output end of the motor 8. A driven gear 18 is fixedly sleeved on the upper end of the main pipe 2. The drive gear 9 and the driven gear 18 mesh and contact each other.
[0031] The motor 8 is started, which drives the drive gear 9 to rotate. The drive gear 9 drives the driven gear 18 to rotate. The driven gear 18 drives the main pipe 2 to rotate. The main pipe 2 drives the multiple stirring rods 3 set on its surface to rotate, which can realize the mixing and stirring of microbial agents.
[0032] See Figure 1 The scraper 6 has an arc-shaped structure on the side near the inner wall of the tank 1, and the scraper 6 is made of silicone rubber.
[0033] The inner wall of the cylindrical tank 1 is an arc-shaped curved surface. The arc-shaped scraper 6 can form a "surface contact" with the inner wall (instead of the "line contact" of the flat scraper), which can cover more of the inner wall area and avoid bacterial liquid and material residue caused by the gap in the fit. It is especially suitable for scenarios with high requirements for "no residue" such as biological cultivation and food processing.
[0034] Silicone rubber has high elasticity and can maintain its shape stability even when it comes into contact with and rubs against the inner wall of the tank for a long time. It is not easy for itself or the tank to wear out excessively due to rigid friction.
[0035] See Figure 1 The other port of the three-way valve 11 is fixedly connected to an air inlet pipe 12. An annular exhaust pipe 13 is fixedly connected to the bottom of the tank body 1. Multiple exhaust nozzles 14 are connected to the annular exhaust pipe 13. The other end of the air inlet pipe 12 is connected to the annular exhaust pipe 13.
[0036] By setting a three-way valve 11, the oxygen delivery can be redirected. Therefore, the oxygen generated by the oxygen supply equipment can be discharged into the annular exhaust pipe 13 through the second air inlet pipe 12, and finally discharged through multiple exhaust nozzles 14, so as to supply oxygen to the bacterial solution prepared by the microbial strains, thereby flexibly realizing the switching between nutrient supply and pressure scraping.
[0037] See Figure 1 A discharge pipe 15 is fixedly installed at the bottom of the tank body 1, and a valve is installed on the discharge pipe 15.
[0038] The bottom of tank 1 is the area where materials naturally settle. The discharge pipe 15 is fixed to the bottom. Bottom discharge allows the materials in the tank (such as cultured bacterial liquid or reaction mixture) to flow along the tank wall to the discharge pipe 15 by their own gravity, and finally be discharged through the discharge pipe 15.
[0039] See Figure 1 A feed pipe 7 is fixedly connected to the top of the tank body 1, and the feed pipe 7 is connected to the inside of the tank body 1.
[0040] In the early stages of cultivation, sterilized liquid culture medium (such as nutrient solution containing carbon and nitrogen sources) can be directly injected into the tank through the feed pipe 7, and a sealing cap can be detachably installed on the top of the feed pipe 7.
[0041] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely preferred examples and are not intended to limit the utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.
Claims
1. A microbial inoculant cultivation and propagation device, characterized in that: The device includes a tank (1) and a vertically arranged main pipe (2) that is rotatably inserted into the top of the tank (1). The lower end of the main pipe (2) is located inside the tank (1). Multiple hollow stirring rods (3) are connected to the lower end of the main pipe (2). A slider (5) is slidably arranged inside the stirring rod (3). The other end of the slider (5) is fixedly connected to a rod (4). The other ends of the multiple rods (4) extend to the outside of the stirring rod (3) and are fixedly connected to a scraper (6). A spring (17) is fixedly connected between the slider (5) and the inner end of the stirring rod (3). The spring (17) is sleeved on the rod (4). When the scraper (6) is pressed, it can come into contact with the inner wall of the tank (1). The top of the tank (1) is fixedly connected to a bracket (16), and an air inlet pipe (10) is fixedly installed on the bracket (16). The other end of the air inlet pipe (10) is inserted into the top of the main pipe (2), and a three-way valve (11) is fixedly installed on the other end of the air inlet pipe (10).
2. The microbial inoculant cultivation and propagation device according to claim 1, characterized in that: A motor (8) is fixedly installed on the top of the tank (1), and a drive gear (9) is fixedly connected to the output end of the motor (8). A driven gear (18) is fixedly sleeved on the upper end of the main pipe (2), and the drive gear (9) meshes with the driven gear (18).
3. The microbial inoculant cultivation and propagation device according to claim 1, characterized in that: The scraper (6) has an arc-shaped structure on the side near the inner wall of the tank (1), and the scraper (6) is made of silicone rubber.
4. The microbial inoculant cultivation and propagation device according to claim 1, characterized in that: The other port of the three-way valve (11) is fixedly connected to an air inlet pipe two (12), and an annular exhaust pipe (13) is fixedly connected to the bottom of the tank (1). Multiple exhaust nozzles (14) are connected to the annular exhaust pipe (13), and the other end of the air inlet pipe two (12) is connected to the annular exhaust pipe (13).
5. The microbial inoculant cultivation and propagation device according to claim 1, characterized in that: A discharge pipe (15) is fixedly installed at the bottom of the tank (1), and a valve is provided on the discharge pipe (15).
6. The microbial inoculant cultivation and propagation device according to claim 1, characterized in that: The top of the tank (1) is fixedly connected to a feed pipe (7), which is connected to the inside of the tank (1).
Citation Information
Patent Citations
Microbial agent cultivation and propagation device
CN219279863U