Hybridoma cell fusion apparatus based on rotatable base and constant temperature water bath

Abstract

This invention provides a hybridoma cell fusion device based on a rotatable base and a constant-temperature water bath, including a constant-temperature water bath container, a rotating base, a transmission mechanism, and a hand-cranked turntable. The rotating base includes a sleeve, a sleeve support, and a rotating gear. The central shaft of the rotating gear is rotatably mounted on the top of the sleeve support, the bottom of the sleeve support is fixedly mounted on the inner wall of the constant-temperature water bath container, the sleeve is fixedly mounted on the top of the central shaft of the rotating gear, and the hand-cranked turntable is mounted on the side of the constant-temperature water bath container. The end of the hand-cranked turntable has a gear that is connected to the rotating gear for transmission. This invention fixes centrifuge tubes to the sleeve of the rotating base, making the height of the centrifuge tubes relative to the water bath liquid level relatively stable and the contact area relatively constant, providing a stable constant-temperature environment for the fusion operation. Furthermore, it simplifies the rotational movements of the fingers and wrists required in the fusion operation to simply cranking the turntable, avoiding the inconvenience caused by fine finger and wrist manipulation, and ensuring that the centrifuge tube rotation speed is relatively stable and controllable.

Description

Technical Field

[0001] This utility model relates to the field of cell fusion technology, specifically to a hybridoma cell fusion device based on a rotatable base and a constant temperature water bath. Background Technology

[0002] The overall experimental process of hybridoma cell fusion is quite complex. Generally, cell fusion needs to be carried out at a constant temperature. In order to ensure that the cells and fusion reagents are in full contact, the experimenter needs to add fusion reagents while slowly rotating the container holding the cells in his hand with his fingers and controlling the time of the whole process. This puts a great test on the experimenter's hand coordination ability. If the experimenter's hand movements are slightly deformed and disturb the container, it will affect the efficiency of hybridoma cell fusion. Therefore, there is an urgent need to provide a fusion device that can achieve both convenient rotation operation and a constant temperature environment. Patent document CN213866148U discloses a hybridoma cell fusion device, relating to the field of biotechnology. It addresses the problem of interference between the drip end cap and the drip tube during manual adjustment of the existing hybridoma cell fusion device, thus preventing drip tube contamination during cell fusion. However, this solution does not improve how to achieve convenient rotational operation under constant temperature conditions. Utility Model Content

[0003] In view of the deficiencies in the existing technology, the purpose of this utility model is to provide a hybridoma cell fusion device based on a rotatable base and a constant temperature water bath.

[0004] The hybridoma cell fusion device based on a rotatable base and a constant temperature water bath provided by this utility model includes a constant temperature water bath container, a rotatable base, a transmission mechanism, a first transmission gear, a hand-cranked turntable, and a second transmission gear. The rotating base includes a sleeve, a sleeve support, and a rotating gear. The central shaft of the rotating gear is rotatably mounted on the top of the sleeve support. The bottom of the sleeve support is fixedly mounted on the inner wall of the constant temperature water bath container. The sleeve is fixedly mounted on the top of the central shaft of the rotating gear for fixing the centrifuge tube. The hand-cranked turntable is installed on the side of the constant temperature water bath container. The end of the hand-cranked turntable has a gear. The first transmission gear and the second transmission gear are respectively installed at both ends of the transmission mechanism. The gear at the end of the hand-cranked turntable meshes with the second transmission gear, and the first transmission gear meshes with the rotating gear. The hand-cranked turntable is used to drive the second transmission gear to rotate through the gear at the end, thereby driving the centrifuge tube inside the sleeve to rotate in sequence through the transmission mechanism, the first transmission gear, and the rotating gear.

[0005] Preferably, the sleeve includes a cylindrical sleeve, which is coaxially and fixedly installed on the top of the central shaft of the rotating gear, and the interior of the cylindrical sleeve is used to engage and fix the centrifuge tube.

[0006] Preferably, the ratio of the radius of the rotating gear to the radius of the first transmission gear is 1:1.8 to 1:2.2.

[0007] Preferably, the ratio of the radius of the gear at the end of the hand-cranked turntable to the radius of the second transmission gear is 1:1.1 to 1:1.4.

[0008] Preferably, the transmission mechanism includes a transmission belt, a belt drive pulley, and a transmission shaft; There are two belt drive pulleys, which are connected by a drive belt. One belt drive pulley is coaxially and fixedly connected to a first drive gear located inside the constant temperature water bath container via a first drive shaft, and the other belt drive pulley is coaxially and fixedly connected to the second drive gear via a second drive shaft.

[0009] Preferably, the two belt-driven pulleys have the same radius.

[0010] Preferably, the gears at the end of the hand-cranked turntable, the second transmission gear, the first transmission gear, and the rotating gear are all bevel gears.

[0011] Preferably, the axis of the gear at the end of the hand-cranked turntable is perpendicular to the axis of the second transmission gear, the axis of the second transmission gear is parallel to the axis of the first transmission gear, and the axis of the first transmission gear is perpendicular to the axis of the rotating gear.

[0012] Preferably, the rotating gear is inclinedly disposed on the top of the sleeve support, and the axis of the rotating gear is inclined upward relative to the horizontal direction in the projection along the radial direction of the rotating gear.

[0013] Preferably, the constant temperature water bath container is equipped with a water bath heating tube and a temperature probe inside; An electronic display screen is installed on the outside of the constant temperature water bath container, and the electronic display screen is electrically connected to the temperature probe.

[0014] Compared with the prior art, the present invention has the following beneficial effects: This invention can fix the centrifuge tubes for fusion onto the sleeve of the rotating base. The height of the centrifuge tubes relative to the water bath liquid surface is relatively stable, and the contact area is relatively constant, providing a stable constant temperature environment for the fusion operation. It also simplifies the centrifuge tube rotation action that originally required the experimenter's fingers to operate in the fusion operation to shaking the hand-cranked turntable, avoiding the inconvenience caused by the fine operation of the fingers and wrists. Moreover, the centrifuge tube rotation speed is relatively stable and controllable. Attached Figure Description

[0015] Other features, objects, and advantages of this invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings: Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a top view of the structure of this utility model; Figure 3 This is a cross-sectional view of the present invention from a side perspective.

[0016] The diagram shows: Detailed Implementation

[0017] The present invention will now be described in detail with reference to specific embodiments. These embodiments will help those skilled in the art to further understand the present invention, but do not limit the present invention in any way. It should be noted that those skilled in the art can make several changes and improvements without departing from the concept of the present invention. These all fall within the protection scope of the present invention.

[0018] This utility model discloses a hybridoma cell fusion device based on a rotatable base and a constant temperature water bath. The centrifuge tubes for fusion are fixed on the sleeve of the rotating base. The height of the centrifuge tubes relative to the water bath liquid surface is relatively stable, and the contact area is relatively constant, providing a stable constant temperature environment for the fusion operation. Furthermore, the centrifuge tube rotation action that originally required the experimenter's fingers to operate during the fusion operation is simplified to shaking a hand-cranked turntable, avoiding the inconvenience caused by the fine operation of the fingers and wrists. Moreover, the centrifuge tube rotation speed is relatively stable and controllable.

[0019] According to the hybridoma cell fusion device based on a rotatable base and a constant temperature water bath provided by this utility model, such as Figures 1-3 As shown, the system includes a constant temperature water bath container, a rotating base 1, a transmission mechanism, a first transmission gear 3, a hand-cranked turntable 7, and a second transmission gear 8. The rotating base 1 includes a sleeve 2, a sleeve support column, and a rotating gear. The central shaft of the rotating gear is rotatably mounted on the top of the sleeve support column, and the bottom of the sleeve support column is fixedly mounted on the inner wall of the constant temperature water bath container. The sleeve 2 is fixedly mounted on the top of the central shaft of the rotating gear and is used to fix the centrifuge tube. By installing the centrifuge tube in the constant temperature water bath container through the sleeve support column, the height of the centrifuge tube relative to the water bath liquid surface can be relatively stable, and the contact area can be relatively constant.

[0020] The hand-cranked turntable 7 is installed on the side of the constant temperature water bath container. The end of the hand-cranked turntable 7 has a gear. The first transmission gear 3 and the second transmission gear 8 are respectively installed at both ends of the transmission mechanism. The gear at the end of the hand-cranked turntable 7 meshes with the second transmission gear 8, and the first transmission gear 3 meshes with the rotating gear. The transmission mechanism includes a transmission belt 4, a belt drive wheel 5, and a transmission shaft 6. There are two belt drive wheels 5, which are connected by the transmission belt 4. The two belt drive wheels 5 have the same radius. One belt drive wheel 5 is coaxially and fixedly connected to the first transmission gear 3 located inside the constant temperature water bath container through the first transmission shaft, and the other belt drive wheel 5 is coaxially and fixedly connected to the second transmission gear 8 through the second transmission shaft 6.

[0021] The working principle of this utility model is as follows: the user shakes the hand crank turntable 7, which drives the gear at its end to rotate. The end gear drives the second transmission gear 8, which meshes with it, to rotate. The second transmission gear 8 drives the belt drive wheel 5, which is coaxial with it, to rotate through the second transmission shaft 6. The belt drive wheel 5 drives another belt drive wheel 5 to rotate through the transmission belt 4. The other belt drive wheel 5 drives the first transmission gear 3, which is coaxial with it, to rotate through the first transmission shaft. The first transmission gear 3 drives the rotating gear to rotate, thereby driving the centrifuge tube inside the sleeve 2 to rotate.

[0022] In a preferred embodiment, the sleeve 2 includes a cylindrical sleeve, which is coaxially and fixedly installed at the top of the central shaft of the rotating gear. The interior of the cylindrical sleeve is used to engage and fix the centrifuge tube. Specifically, the diameter of the cylindrical sleeve is 3cm±1mm, designed to just hold a 50ml centrifuge tube.

[0023] In a preferred embodiment, the radius of the rotating gear is in the ratio of 1:1.8 to 1:2.2 to the radius of the first transmission gear 3, and the radius of the rotating gear is 5.5cm to 7.5cm. The belt drive wheel 5, which is connected to the hand crank turntable 7, has a second transmission shaft on its central shaft. The length of the second transmission shaft is between 15 and 25cm, and a smaller second transmission gear 8 with a diameter of 3-5cm is provided at the end of the second transmission shaft. The radius of the gear at the end of the hand crank turntable 7 is in the ratio of 1:1.1 to 1:1.4 to the radius of the second transmission gear 8.

[0024] In a preferred embodiment, the gear at the end of the hand-cranked turntable 7, the second transmission gear 8, the first transmission gear 3, and the rotating gear are all bevel gears. The axis of the gear at the end of the hand-cranked turntable 7 is perpendicular to the axis of the second transmission gear 8, the axis of the second transmission gear 8 is parallel to the axis of the first transmission gear 3, and the axis of the first transmission gear 3 is perpendicular to the axis of the rotating gear. The rotation of the hand-cranked turntable 7 located outside the constant temperature water bath container can be transmitted to the sleeve 2 located inside the constant temperature water bath container through the transmission mechanism, so that the centrifuge tube can rotate stably.

[0025] In a preferred embodiment, the rotating gear is inclined at the top of the sleeve support, and the axis of the rotating gear is inclined upward relative to the horizontal direction in the projection along the radial direction of the rotating gear.

[0026] In a preferred embodiment, the constant temperature water bath container is provided with a water bath heating tube 11 and a temperature probe 9 inside; the constant temperature water bath container is provided with an electronic display screen 10 outside, and the electronic display screen 10 is electrically connected to the temperature probe 9.

[0027] Example 1 This embodiment can be understood as a preferred example of the above embodiments, providing a hybridoma cell fusion device with a rotatable base and a constant temperature water bath, such as... Figures 1 to 3 As shown, it includes: Rotating base 1: The top is an inclined rotating gear, and there is a sleeve 2 for fixing centrifuge tubes on the gear shaft. The shaft of the gear shaft is fixed to the bottom surface of the water bath container through the sleeve 2 and the sleeve support. The teeth of the gear are tangent to the lower side of a larger first transmission gear 3. 4. Transmission belt, 5. Two belt drive pulleys, 6. The second transmission shaft 6 has a fixed sleeve in the middle that is connected to the outer metal shell of the constant temperature water bath container. Hand-cranked turntable 7: The hand-cranked turntable 7 is a turntable with a handle. A small gear is connected to the central shaft of the hand-cranked turntable. This gear is tangent to the side of the second transmission gear 8. The second transmission gear 8 is connected to the central shaft of the belt drive pulley 5 through a long second transmission shaft 6. Temperature probe 9, water bath heating element 11, and electronic display screen 10. The electronic display screen 10 is used to display the current temperature of the water bath, and there are power buttons and buttons below it to control the temperature.

[0028] The following further explains how to use this device, including the following steps: Turn on the power button below the electronic display screen 10, set the water temperature to the desired temperature, and wait for the temperature to stabilize. Insert the 50ml centrifuge tube containing the hybridoma cells to be fused into the sleeve 2. Rotate the hand crank 7 at a uniform speed. The small gear connected to the central shaft of the hand crank 7 drives the second transmission gear 8 to rotate. The second transmission gear 8 drives the belt drive wheel 5 to rotate through the second transmission shaft 6. The belt drive wheel 5 drives the first transmission gear 3 to rotate. The first transmission gear 3 drives the rotating base 1 to rotate, ultimately causing the hybridoma cells in the centrifuge tube to be in a slow, adherent flow state.

[0029] This invention can fix the centrifuge tubes for fusion onto the sleeve of the rotating base. The height of the centrifuge tubes relative to the water bath liquid surface is relatively stable, and the contact area is relatively constant, providing a stable environment for the fusion operation. It also simplifies the centrifuge tube rotation that originally required the experimenter's fingers to operate by simply shaking a hand-cranked turntable, avoiding the inconvenience and fatigue of fine finger and wrist operations. Furthermore, the centrifuge tube rotation speed is relatively stable and controllable.

[0030] The device of this invention occupies a small overall space, with a length, width and height of approximately 30cm × 20cm × 15cm. It can be easily placed in a single-person clean bench. The transmission wheel and gears and other devices in this invention are detachable, and the device can also be used as a simple water bath, which improves its convenience and practicality.

[0031] In the description of this application, it should be understood that the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0032] The specific embodiments of this utility model have been described above. It should be understood that this utility model is not limited to the specific embodiments described above, and those skilled in the art can make various changes or modifications within the scope of the claims, which do not affect the substantive content of this utility model. Unless otherwise specified, the embodiments and features described in this application can be arbitrarily combined with each other.

Claims

1. A hybridoma cell fusion apparatus based on a rotatable base and a thermostatic water bath, characterized in that, The device comprises a constant temperature water bath container, a rotating base (1), a transmission mechanism, a first transmission gear (3), a hand-operated rotary disc (7) and a second transmission gear (8). The rotating base (1) comprises a sleeve (2), a sleeve support and a rotating gear, the central shaft of the rotating gear is rotatably installed on the top of the sleeve support, the bottom of the sleeve support is fixedly installed on the inner wall of the constant temperature water bath container, the sleeve (2) is fixedly installed on the top of the central shaft of the rotating gear and used for fixing the centrifugal tube. The hand-operated rotary disc (7) is installed on the side of the constant temperature water bath container, the end of the hand-operated rotary disc (7) is provided with a gear, the first transmission gear (3) and the second transmission gear (8) are respectively installed on the two ends of the transmission mechanism, the gear at the end of the hand-operated rotary disc (7) is engaged with the second transmission gear (8), and the first transmission gear (3) is engaged with the rotating gear. The hand-operated rotary disc (7) is used for driving the second transmission gear (8) to rotate through the gear at the end, so as to drive the centrifugal tube in the sleeve (2) to rotate through the transmission mechanism, the first transmission gear (8) and the rotating gear in sequence.

2. A hybridoma cell fusion apparatus based on a rotatable base and a thermostatic water bath according to claim 1, characterized in that, The sleeve (2) comprises a cylindrical sleeve, the cylindrical sleeve is coaxially and fixedly installed on the top end of the central shaft of the rotating gear, and the inside of the cylindrical sleeve is used for clamping and fixing the centrifugal tube.

3. The rotatable base and constant temperature water bath based hybridoma cell fusion apparatus of claim 1, wherein, The ratio of the radius of the rotating gear to the radius of the first transmission gear (3) is 1:1.8-1:2.

2.

4. The rotatable base and constant temperature water bath based hybridoma cell fusion apparatus of claim 1, wherein, The ratio of the radius of the gear at the end of the hand-operated rotary disc (7) to the radius of the second transmission gear (8) is 1:1.1-1:1.

4.

5. The rotatable base and constant temperature water bath based hybridoma cell fusion apparatus of claim 1, wherein, The transmission mechanism comprises a transmission belt (4), a belt transmission wheel (5) and a transmission shaft (6). The belt transmission wheel (5) is provided with two, the two belt transmission wheels (5) are transmissionally connected through the transmission belt (4), one of the belt transmission wheels (5) is coaxially and fixedly connected with the first transmission gear (3) in the constant temperature water bath container through a first transmission shaft, and the other belt transmission wheel (5) is coaxially and fixedly connected with the second transmission gear (8) through a second transmission shaft (6).

6. A hybridoma cell fusion apparatus based on a rotatable base and a thermostatic water bath according to claim 5, characterized in that, The radii of the two belt transmission wheels (5) are the same.

7. The rotatable base and constant temperature water bath based hybridoma cell fusion apparatus of claim 5, wherein, The gear at the end of the hand-operated rotary disc (7), the second transmission gear (8), the first transmission gear (3) and the rotating gear are all bevel gears.

8. A hybridoma cell fusion apparatus based on a rotatable base and a thermostatic water bath according to claim 7, characterized in that, The axis of the gear at the end of the hand-operated rotary disc (7) is perpendicular to the axis of the second transmission gear (8), the axis of the second transmission gear (8) is parallel to the axis of the first transmission gear (3), and the axis of the first transmission gear (3) is perpendicular to the axis of the rotating gear.

9. The rotatable base and constant temperature water bath based hybridoma cell fusion apparatus of claim 1, wherein, The rotating gear is obliquely arranged on the top of the sleeve support, and the axis of the rotating gear is inclined upward compared with the horizontal direction in the projection along the radial direction of the rotating gear.

10. The rotatable base and constant temperature water bath based hybridoma cell fusion apparatus of claim 1, wherein, The inside of the constant temperature water bath container is provided with a water bath tank heating pipe (11) and a temperature probe (9). The outside of the constant temperature water bath container is provided with an electronic display screen (10), and the electronic display screen (10) is electrically connected with the temperature probe (9).

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