A reagent bottle for a cell culture kit

Abstract

This utility model relates to a reagent bottle for a cell culture kit, comprising: a reagent bottle body, a duckbill valve, and a piston assembly; the reagent bottle body includes an open end and a closed end, the open end is provided with a duckbill valve, and the closed end has a through hole on its surface; the piston assembly is disposed inside the reagent bottle body, passes through the through hole, and extends to the outside of the reagent bottle body. This utility model, by setting up the reagent bottle body, duckbill valve, and piston assembly, and through the cooperation between the reagent bottle body and the piston assembly, allows the solvent inside the reagent bottle body to flow out from the duckbill valve when the piston assembly is pushed to move along the axial direction of the reagent bottle body. Furthermore, the duckbill valve is designed so that after the solvent flows out, if the pushing stops, the duckbill valve automatically closes, preventing dust and microorganisms from contaminating the solvent inside the reagent bottle body, thus providing a protective function.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a reagent bottle for a cell culture kit. Background Technology

[0002] Cell culture refers to the technique of artificially simulating the physiological environment in vivo (such as temperature, humidity, nutrients, and gaseous environment) in vitro, enabling cells to continue to grow, proliferate, differentiate, and maintain specific functions after being removed from the body. It is one of the core technologies in modern life science research, biomedical development, and clinical diagnosis. Its essence is to "make cells 'survive and work' in the laboratory," providing a controllable experimental model for studying cell physiological mechanisms, disease occurrence and development, and drug screening.

[0003] Cell culture kits are combinations of reagents used in the cell culture process, containing a variety of components required for cell growth, maintenance, and differentiation. The kits provide a standardized, adaptable, and convenient solution for cell culture, thereby solving problems such as "difficulty in nutrient ratio, high risk of contamination, and unstable cell state" in traditional cell culture, and ultimately ensuring cell viability, function, and experimental reproducibility. The kit contains many components, among which reagent bottles are used to store different liquid reagents. When using common reagent bottles, opening the cap will expose the bottle opening and reagent surface directly to the air, making them susceptible to contamination by dust and microorganisms. The reagent bottles are of fixed sizes (50ml, 100ml). If only a small amount of reagent is needed for the experiment (such as 10ml for cell resuscitation), the remaining reagent may have to be discarded due to its short shelf life after opening (such as culture medium stored at 4°C for no more than 2 weeks). For example, in kits specifically designed for "primary cell culture", due to the low yield of primary cells and the small scale of the experiment, more than 80% of the reagent often becomes ineffective before it is used up. To extend the shelf life of some reagents, "aseptic dispensing" is a common method, which involves "aseptic dispensing" immediately after opening. However, this method has high requirements for operation and environment. Therefore, a reagent bottle for cell culture kits is proposed to solve the above-mentioned problems. Utility Model Content

[0004] Based on the above description, this utility model provides a reagent bottle for cell culture kits to solve the problem that the remaining solvent in the reagent bottle is contaminated after opening and use, causing the solvent to become ineffective before it is used up, because the reagent bottle contains a lot of solvent during the cell culture process.

[0005] The technical solution of this utility model to solve the above-mentioned technical problems is as follows: A reagent bottle for a cell culture kit, comprising: a reagent bottle body, a duckbill valve and a piston assembly; The reagent bottle body includes an open end and a closed end. A duckbill valve is provided at the open end, and a through hole is provided on the surface of the closed end. The piston assembly is disposed inside the reagent bottle body and extends through the through hole to the outside of the reagent bottle body.

[0006] Based on the above technical solution, the present invention can be further improved as follows.

[0007] Furthermore, the reagent bottle body includes a bottle body, the open end of the bottle body is provided with a hollow threaded cylinder, and the circumferential surface is provided with scale lines, and the closed end of the bottle body is provided with a through hole.

[0008] Furthermore, the duckbill valve includes an installation cylinder, which is disposed inside the hollow threaded cylinder, and a duckbill valve body is disposed at the end opposite to the through hole. A limiting ring is sleeved on the outside of the duckbill valve body, and the limiting ring contacts the surface of the hollow threaded cylinder at the end opposite to the through hole. The installation cylinder and the hollow threaded cylinder are interference-fitted.

[0009] Furthermore, the hollow threaded cylinder is fitted with a cap, the cap including a cap body, the valve body of the duckbill valve extending into the interior of the cap body, and the end of the cap body facing the reagent bottle body is provided with an internal threaded hole, the internal threaded hole being threadedly connected to the hollow threaded cylinder.

[0010] Furthermore, the piston assembly includes a piston block, which includes a block disposed inside the bottle body. The circumferential surface of the block is provided with an annular groove, and a sealing ring is disposed inside the annular groove. The outer ring of the sealing ring is tightly fitted with the inner wall of the bottle body to seal the block and the inner wall of the bottle body.

[0011] Furthermore, the diameter of the block is larger than the inner diameter of the through hole, and a threaded blind hole is provided on the surface facing one end of the through hole, the threaded blind hole being located at the center of the through hole.

[0012] Furthermore, a circular groove is provided on the surface of the block away from the through hole. The circular groove is located at the center of the block. A vent hole is provided along the outer edge of the circular groove. A hydrophobic membrane is provided inside the circular groove.

[0013] Furthermore, a rod is provided at one end of the piston block facing the through hole. The rod includes a long rod and a fixing member, both of which can be threadedly connected to the block.

[0014] Furthermore, the long rod includes a rod body, which is disposed on the outside of the bottle body, passes through the through hole and extends into the inside of the bottle body, and a first threaded post is provided at one end of the rod body facing the block, the first threaded post being threadedly connected to the threaded blind hole.

[0015] Furthermore, the fixing component includes a stop block, which is disposed on the outside of the bottle body and has an outer diameter larger than the inner diameter of the through hole. A second threaded post is provided at one end of the stop block facing the block body. The second threaded post passes through the through hole and is threadedly connected to the threaded blind hole.

[0016] Compared with the prior art, the technical solution of this application has the following beneficial technical effects: 1. This utility model, by setting up a reagent bottle body, a duckbill valve, and a piston assembly, etc., through the cooperation between the reagent bottle body and the piston assembly, allows the solvent inside the reagent bottle body to flow out from the duckbill valve when the piston assembly is pushed to move along the axial direction of the reagent bottle body. Furthermore, the duckbill valve is designed so that if the pushing stops after the solvent flows out, the duckbill valve will automatically close, thus preventing dust and microorganisms from contaminating the solvent inside the reagent bottle body. 2. By setting up the fixing part and the long rod, the effect of changing the state of the reagent bottle is achieved. When the fixing part is connected to the piston block, it fixes the piston block, so that when the reagent bottle moves with the reagent kit, it avoids solvent leakage due to external factors such as bumps. The setting of the long rod allows the reagent bottle to push the long rod to drive the piston block to move together when in use, thereby allowing the solvent to be pushed out. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of a reagent bottle for a cell culture kit provided in an embodiment of the present invention; Figure 2 for Figure 1 Structural sectional view; Figure 3 for Figure 2 Another structural diagram from a different perspective; Figure 4 This is a schematic diagram of the structure of the reagent bottle body in an embodiment of this utility model; Figure 5 This is a schematic diagram of the structure of the cap in an embodiment of this utility model; Figure 6 This is a schematic diagram of the duckbill valve in an embodiment of the present invention; Figure 7 This is a schematic diagram of the piston block in an embodiment of the present invention; Figure 8 for Figure 7 Another structural diagram from a different perspective; Figure 9 This is a schematic diagram of the block structure in an embodiment of the present utility model; Figure 10 This is a schematic diagram of the long rod in an embodiment of the present invention; Figure 11 This is a schematic diagram of the structure of the fixing component in an embodiment of this utility model; Figure 12 This is a schematic diagram showing the usage state of the fastener in the embodiments of this utility model; The attached diagram lists the components represented by each number as follows: 1. Reagent bottle body; 11. Bottle body; 12. Graduation line; 13. Hollow threaded cylinder; 14. Through hole; 2. Cap; 21. Cap body; 22. Internal threaded hole; 3. Duckbill valve; 31. Duckbill valve body; 32. Mounting cylinder; 33. Limiting ring; 4. Piston block; 41. Block body; 42. Sealing ring; 43. Hydrophobic membrane; 44. Annular groove; 45. Threaded blind hole; 46. Circular groove; 47. Vent hole; 5. Long rod; 51. Rod body; 52. First threaded post; 6. Fixing component; 61. Stop block; 62. Second threaded post. Detailed Implementation

[0018] To facilitate understanding of this application, a more complete description will be provided below with reference to the accompanying drawings, which illustrate embodiments of the present application. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that the disclosure of this application will be thorough and complete.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application.

[0020] When used herein, the singular forms of “a,” “an,” and “the” may also include the plural forms unless the context clearly indicates otherwise. It should also be understood that the terms “comprising,” “including,” or “having,” etc., specify the presence of the stated feature, whole, step, operation, component, part, or combination thereof, but do not preclude the possibility of the presence or addition of one or more other features, wholes, steps, operations, components, parts, or combinations thereof.

[0021] Please see Figures 1-4 A reagent bottle for a cell culture kit includes: a reagent bottle body 1, a duckbill valve 3, and a piston assembly; The reagent bottle body 1 includes an open end and a closed end. A duckbill valve 3 is provided at the open end, and a through hole 14 is provided on the surface of the closed end. The piston assembly is disposed inside the reagent bottle body 1, and extends through the through hole 14 to the outside of the reagent bottle body 1. The reagent bottle body 1 includes a bottle body 11, which is preferably made of medical PET material. The open end of the bottle body 11 is provided with a hollow threaded cylinder 13, and a scale line 12 is provided on the circumferential surface. The closed end of the bottle body 11 is provided with a through hole 14. Based on the above, the reagent bottle body 1 serves to store solvent. The hollow threaded cylinder 13 is connected to the duckbill valve 3 by interference fit through its inner wall, and is connected to the cap 2 by threaded connection on the outside. The through hole 14 allows the piston assembly to extend to the outside of the reagent bottle body 1. The piston assembly allows the user to push the piston assembly to allow the solvent inside the reagent bottle body 1 to flow out from the duckbill valve 3.

[0022] like Figure 2 , Figure 3 as well as Figure 6 As shown, the duckbill valve 3 includes an installation cylinder 32, which is disposed inside the hollow threaded cylinder 13, and a duckbill valve body 31 is provided at the end opposite to the through hole 14. The duckbill valve body 31 is preferably made of fluororubber, and a limiting ring 33 is sleeved on the outside of the duckbill valve body 31. The limiting ring 33 is in contact with the surface of the hollow threaded cylinder 13 at the end opposite to the through hole 14, and the installation cylinder 32 is interference-fitted with the hollow threaded cylinder 13. Based on the above, the duckbill valve 3 acts as a one-way valve, allowing the solvent to flow out from the duckbill valve 3 in one direction after being pushed by the piston assembly. After the piston assembly stops moving, the duckbill valve 3 automatically closes, thus providing protection. The limiting ring 33 and the hollow threaded cylinder 13 cooperate with each other to play a limiting role, so that the position of the duckbill valve 3 is constant when it is installed. The mounting cylinder 32 is connected to the hollow threaded cylinder 13 by interference fit to achieve the effect of fixing the duckbill valve 3.

[0023] like Figures 1-3 as well as Figure 5 As shown, the hollow threaded cylinder 13 is fitted with a cap 2, the cap 2 includes a cap body 21, the duckbill valve body 31 extends into the inside of the cap body 21, and the cap body 21 is provided with an internal threaded hole 22 at one end facing the reagent bottle body 1, the internal threaded hole 22 is threadedly connected to the hollow threaded cylinder 13. Based on the above, the cap 2 is connected to the hollow threaded cylinder 13 by a threaded connection. At this time, the cap 2 plays a protective role, that is, it protects the duckbill valve 3 and prevents external forces from acting directly on the duckbill valve 3, thereby preventing solvent leakage caused by the duckbill valve 3 being directly subjected to force.

[0024] like Figure 2 , Figure 3 , Figures 7-9 As shown, the piston assembly includes a piston block 4, which includes a block 41 disposed inside the bottle body 11. The circumferential surface of the block 41 is provided with an annular groove 44, and a sealing ring 42 is disposed inside the annular groove 44. The sealing ring is preferably made of butyl rubber. The outer ring of the sealing ring 42 is tightly fitted with the inner wall of the bottle body 11 in an interference fit state to seal the block 41 and the inner wall of the bottle body 11. The diameter of the block 41 is larger than the inner diameter of the through hole 14, and a threaded blind hole 45 is provided on the surface facing the through hole 14. The threaded blind hole 45 is located at the center of the through hole 14. A circular groove 46 is provided on the surface of the block 41 away from the through hole 14. The circular groove 46 is located at the center of the block 41. A vent hole 47 is provided along the outer edge of the circular groove 46. The vent hole diameter is preferably 0.1μm. A hydrophobic membrane 43 is provided inside the circular groove 46. The hydrophobic and breathable membrane is preferably made of PTFE material. Based on the above, the piston block 4 is sealed by the sealing ring 42, so that when the piston block 4 moves along the axial direction of the bottle body 11, it can push the solvent inside to move together. Furthermore, the hydrophobic membrane 43 and the vent 47 cooperate with each other, so that the solvent and microorganisms cannot come into contact with the solvent through the hydrophobic membrane 43, while the vent 47 plays the role of balancing the air pressure, so that the air pressure inside the bottle body 11 is constant.

[0025] like Figures 1-3 , Figure 10 As shown, a rod is provided at one end of the piston block 4 facing the through hole 14. The rod includes a long rod 5 and a fixing member 6. Both the long rod 5 and the fixing member 6 can be threadedly connected to the block 41. The long rod 5 includes a rod body 51, which is disposed on the outside of the bottle body 11, passes through the through hole 14 and extends into the inside of the bottle body 11. A first threaded post 52 is provided at one end of the rod body 51 facing the block 41, and the first threaded post 52 is threadedly connected to the threaded blind hole 45. Based on the above, the long rod 5 is connected to the piston block 4 by the cooperation of the first threaded post 52 and the threaded blind hole 42. At this time, by pushing the long rod 5, the piston block 4 is driven to move along the axial direction of the bottle body 11, thereby achieving the effect of pushing the solvent out.

[0026] like Figure 11 and Figure 12As shown, the fixing member 6 includes a stop block 61, which is disposed on the outside of the bottle body 11 and has an outer diameter larger than the inner diameter of the through hole 14. A second threaded post 62 is provided at one end of the stop block 61 facing the block body 41. The second threaded post 62 passes through the through hole 14 and is threadedly connected to the threaded blind hole 45. Based on the above, the fixing member 6 is connected to the piston block 4 by a threaded connection, and at this time the reagent bottle is in the state of the reagent kit. Figure 12 This is the initial state. Figure 1 The state in the image represents the state of the reagent bottle when it is in use. The stop block 61 serves to limit the piston block 4, preventing the piston block 4 from moving along the axial direction of the bottle body 11 after it is connected to the fixing part 6. In summary, this reagent bottle, as part of the reagent kit, is mostly a single-use item (after the staff purchases the kit, the reagent bottle filled with solvent is already inside the kit, i.e., ...). Figure 12 The state in the middle is the initial state of the reagent bottle. At this time, the reagent bottle plays the role of storing solvent. During this process, the fixing part 6 plays the role of fixing the piston block 4. In addition, the reagent bottle body 1, together with the cap 2, protects the duckbill valve 3, thereby ensuring that the reagent bottle will not leak solvent during transportation. After the reagent bottle is removed from the reagent kit, if it is needed, it can be removed by unscrewing the fixing part 6 in the reverse direction. Then, the long rod 5 is connected to the piston block 4 through the threaded connection, and the cap 2 is unscrewed. At this time, the piston block 4 can be moved closer to the duckbill valve 3 by pushing the long rod 5. During this process, the solvent inside the bottle body 11 flows out from the inside of the duckbill valve 3 under the push of the piston block 4, thereby achieving the effect of taking out the solvent for use. When the long rod 5 is stopped, the piston block 4 also stops moving. As a result, the solvent stops flowing out of the duckbill valve 3 after losing external force. At this time, the duckbill valve 3 returns to its original state, that is, the valve opening is closed, thereby isolating the external environment from the solvent and preventing dust and microorganisms from coming into contact with the solvent inside the bottle 11, thus extending the usage time of the remaining solvent. The design of this reagent bottle, with its duckbill valve 3, piston block 4, and long rod 5, not only allows for controllable solvent dispensing but also prevents external air from contacting the solvent inside the bottle 11 during dispensing. After dispensing, the duckbill valve 3 automatically closes, further protecting the solvent inside the bottle 11. By minimizing contact between the solvent and air, the bottle extends its shelf life (this extended shelf life is based on the premise that other storage conditions are met, allowing the solvent to be stored for a longer period compared to traditional reagent bottles).

[0027] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A reagent bottle for a cell culture kit, characterized in that, include: The reagent bottle body (1), the duckbill valve (3), and the piston assembly; The reagent bottle body (1) includes an open end and a closed end. A duckbill valve (3) is provided at the open end, and a through hole (14) is provided on the surface of the closed end. The piston assembly is disposed inside the reagent bottle body (1), and extends through the through hole (14) to the outside of the reagent bottle body (1).

2. The reagent bottle for the cell culture kit according to claim 1, characterized in that, The reagent bottle body (1) includes a bottle body (11), the open end of the bottle body (11) is provided with a hollow threaded cylinder (13), and a scale line (12) is provided on the circumferential surface. The closed end of the bottle body (11) is provided with a through hole (14).

3. The reagent bottle for the cell culture kit according to claim 2, characterized in that, The duckbill valve (3) includes an installation cylinder (32), which is located inside the hollow threaded cylinder (13) and has a duckbill valve body (31) at one end away from the through hole (14). A limiting ring (33) is sleeved on the outside of the duckbill valve body (31). The limiting ring (33) is in contact with the surface of the hollow threaded cylinder (13) at the end away from the through hole (14). The installation cylinder (32) and the hollow threaded cylinder (13) are interference-fitted.

4. The reagent bottle for the cell culture kit according to claim 3, characterized in that, The hollow threaded cylinder (13) is fitted with a cap (2), the cap (2) includes a cap body (21), the duckbill valve body (31) extends into the inside of the cap body (21), and the cap body (21) is provided with an internal thread hole (22) at one end facing the reagent bottle body (1), the internal thread hole (22) is threadedly connected to the hollow threaded cylinder (13).

5. The reagent bottle for the cell culture kit according to claim 2, characterized in that, The piston assembly includes a piston block (4), which includes a block (41) disposed inside the bottle body (11). The circumferential surface of the block (41) is provided with an annular groove (44), and a sealing ring (42) is disposed inside the annular groove (44). The outer ring of the sealing ring (42) is tightly fitted with the inner wall of the bottle body (11) to seal between the block (41) and the inner wall of the bottle body (11).

6. The reagent bottle for the cell culture kit according to claim 5, characterized in that, The diameter of the block (41) is larger than the inner diameter of the through hole (14), and a threaded blind hole (45) is provided on the surface facing one end of the through hole (14), the threaded blind hole (45) being located at the center of the through hole (14).

7. The reagent bottle for the cell culture kit according to claim 6, characterized in that, A circular groove (46) is provided on the surface of the block (41) away from the through hole (14). The circular groove (46) is located at the center of the block (41). A vent hole (47) is provided along the outer edge of the circular groove (46). A hydrophobic membrane (43) is provided inside the circular groove (46).

8. The reagent bottle for the cell culture kit according to claim 6, characterized in that, The piston block (4) has a rod at one end facing the through hole (14). The rod includes a long rod (5) and a fixing member (6). Both the long rod (5) and the fixing member (6) can be threadedly connected to the block (41).

9. The reagent bottle for the cell culture kit according to claim 8, characterized in that, The long rod (5) includes a rod body (51), which is located outside the bottle body (11), passes through the through hole (14) and extends into the inside of the bottle body (11). A first threaded post (52) is provided at one end of the rod body (51) facing the block (41), and the first threaded post (52) is threadedly connected to the threaded blind hole (45).

10. The reagent bottle for the cell culture kit according to claim 8, characterized in that, The fixing member (6) includes a stop block (61), which is located outside the bottle body (11) and has an outer diameter larger than the inner diameter of the through hole (14). A second threaded post (62) is provided at one end of the stop block (61) facing the block body (41). The second threaded post (62) passes through the through hole (14) and is threadedly connected to the threaded blind hole (45).

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