A hand-held fluorescent quantitative PCR circulating tumor cell analyzer

Abstract

The utility model relates to medical instrument technical field especially a kind of hand-held fluorescent quantitative PCR circulating tumor cell analyzer, including pistol type shell, vertical three-layer module cabin, sample start key and microfluidic chip;Pistol type shell is constituted by handle and gun body, the gun body front part is equipped with openable hatch;Vertical three-layer module cabin is located in the openable hatch inside, from top to bottom includes;Upper microfluidic chip slot;Middle layer micro-temperature control system;Lower fluorescent detection module etc.The positive effect that the present application has: portable efficient, pistol type shell design realizes single-hand holding operation, satisfies bedside detection requirement;Quick response, micro-temperature control system completes temperature regulation within 5 seconds, shortens detection cycle;Handle built-in lithium battery supports continuous work 8 hours, is suitable for field medical scene.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a handheld fluorescence quantitative PCR circulating tumor cell analyzer. Background Technology

[0002] Current CTC detection relies on large laboratory equipment, requiring multiple steps of isolation, amplification, and detection, which is time-consuming, costly, and cannot be performed at the bedside. Existing portable PCR instruments generally lack CTC-specific capture and nucleic acid extraction modules, making it difficult to meet the needs of point-of-care diagnosis. The existing patent number CN202220646804.9 proposes a portable ultra-fast real-time fluorescence quantitative PCR instrument, but the chip replacement is cumbersome. Utility Model Content

[0003] The purpose of this invention is to provide a handheld fluorescence quantitative PCR circulating tumor cell analyzer in order to solve the above-mentioned technical problems.

[0004] To achieve the above objectives, this utility model specifically adopts the following technical solution: This invention proposes a handheld fluorescence quantitative PCR circulating tumor cell analyzer, comprising a pistol-shaped shell, a vertical three-layer modular compartment, a sample start button, and a microfluidic chip; The pistol-type housing consists of a grip and a receiver, with an openable hatch at the front of the receiver. A vertical three-layer modular compartment, located inside the openable hatch, includes, from top to bottom: Upper layer: microfluidic chip slot; Middle layer: micro temperature control system; Lower layer: fluorescence detection module; The sample activation button is located at the connection between the grip and the main body of the gun; the microfluidic chip is detachably inserted into the microfluidic chip slot, and its interior integrates a spiral microchannel.

[0005] As a preferred technical solution of this utility model, the openable hatch is connected to the main body of the gun via a magnetic buckle.

[0006] As a preferred technical solution of this utility model, a touch screen is embedded in the top of the gun body, and a USB-C data interface is provided at the rear of the openable hatch.

[0007] As a preferred embodiment of this utility model, the grip has a built-in lithium battery and an anti-slip texture on its outer surface.

[0008] As a preferred technical solution of this utility model, the side wall of the gun body is provided with heat dissipation fins.

[0009] As a preferred embodiment of this utility model, the miniature temperature control system includes a semiconductor cooling chip and a thermocouple, with a temperature control response time of ≤5 seconds and an accuracy of ±0.5℃.

[0010] As a preferred technical solution of this utility model, the fluorescence detection module includes a 45° reflector, a three-color LED light source and a photoelectric sensor, and the light source wavelengths include 470nm, 530nm and 640nm.

[0011] The beneficial effects of this utility model are as follows: Portable and efficient, the pistol-style shell design allows for one-handed operation, meeting bedside testing requirements; 2. Rapid response: The miniature temperature control system completes temperature adjustment within 5 seconds, shortening the detection cycle; 3. The built-in lithium battery in the grip supports 8 hours of continuous operation, making it suitable for field medical scenarios. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the structure of this utility model.

[0013] Reference numerals: 1. Pistol-shaped casing; 2. Vertical three-layer modular compartment; 3. Sample activation button; 4. Microfluidic chip; 11. Grip; 12. Gun body; 13. Openable and closable door; 14. Touch screen; 15. USB-C data interface; 16. Lithium battery; 21. Microfluidic chip slot; 22. Miniature temperature control system; 23. Fluorescence detection module; 41. Spiral microchannel. Detailed Implementation

[0014] like Figure 1 As shown, this utility model proposes: a handheld fluorescence quantitative PCR circulating tumor cell analyzer, comprising a pistol-shaped shell, a vertical three-layer module compartment, a sample start button, and a microfluidic chip; The pistol-type housing consists of a grip and a receiver, with an openable hatch at the front of the receiver. A vertical three-layer modular compartment, located inside the openable hatch, includes, from top to bottom: Upper layer: microfluidic chip slot; Middle layer: micro temperature control system; Lower layer: fluorescence detection module; The sample activation button is located at the connection between the grip and the main body of the gun; the microfluidic chip is detachably inserted into the microfluidic chip slot, and its interior integrates a spiral microchannel.

[0015] The openable hatch is connected to the main body of the gun via a magnetic buckle.

[0016] The gun body has a touch screen embedded in the top and a USB-C data interface at the rear of the openable hatch.

[0017] The grip has a built-in lithium battery and an anti-slip texture on its outer surface.

[0018] The gun body has heat dissipation fins on its side wall.

[0019] The miniature temperature control system includes a semiconductor cooling chip and a thermocouple, with a temperature control response time of ≤5 seconds and an accuracy of ±0.5℃.

[0020] The fluorescence detection module includes a 45° reflector, a three-color LED light source, and a photoelectric sensor. The light source wavelengths include 470nm, 530nm, and 640nm.

[0021] Working principle: Whole blood samples are injected through the inlet at the tail of the microfluidic chip, flowing into the built-in helical microchannel. The helical structure increases the contact area and residence time between blood and antibodies, specifically capturing circulating tumor cells (CTCs). Captured CTCs are transported through the microchannel to the outlet, triggering a piezoelectric ceramic lysis unit. Within 3 seconds, the cells are physically broken, releasing nucleic acids and avoiding interference from residual chemical lysis agents in subsequent PCR. The lysis buffer flows into the lower amplification zone. A micro temperature control system enables rapid temperature cycling (95℃→55℃→72℃) at a rate >8℃ / s, completing 40 amplification cycles in 8 minutes. During amplification, DNA fragments of tumor markers are labeled with fluorescent probes. The amplified products are excited by a tri-color LED light source, emitting fluorescent signals that are refracted by a 45° reflector to a photoelectric sensor, where they are converted into electrical signals in real time. The touchscreen displays the Ct value and the number of CTCs, with a detection limit as low as 1 CTC / mL. After pressing the sample start button, the device automatically completes the entire process, which is 6 times faster than traditional laboratory methods. Example 1:

[0022] Chip loading: Open the hatch and insert the microfluidic chip into the slot; Sample injection: Inject 1 mL of anticoagulated blood through the chip inlet using a microsyringe; Startup check: Press the start button, and the device will automatically perform the following: CTC capture → Cell lysis → qPCR amplification; Reading results: The touchscreen displays the number of CTCs and gene mutation information.

[0023] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. It will be apparent to those skilled in the art that this utility model is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or basic characteristics of this utility model. Therefore, the embodiments should be considered exemplary and non-limiting in all respects. The scope of this utility model is defined by the appended claims rather than the foregoing description, and thus all variations falling within the meaning and scope of equivalents of the claims are intended to be included within this utility model. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0024] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A hand-held, fluorescent quantitative PCR-cycling tumor cell analyzer, characterized by: It includes a pistol-shaped housing (1), a vertical three-layer module compartment (2), a sample start button (3), and a microfluidic chip (4). The pistol-type housing (1) is composed of a grip (11) and a gun body (12), and the gun body (12) is provided with an openable hatch (13) at the front. A vertical three-layer modular compartment (2), located inside the openable hatch (13), comprises, from top to bottom: Upper microfluidic chip slot (21); Middle micro temperature control system (22); Lower fluorescence detection module (23); The sample start button (3) is located at the connection between the grip (11) and the main body of the gun body (12); the microfluidic chip (4) is detachably inserted into the slot of the microfluidic chip (4), and its interior integrates a spiral microchannel (41).

2. The handheld real-time PCR circulating tumor cell analyzer according to claim 1, characterized in that: The openable hatch (13) is connected to the main body of the gun body (12) by a magnetic buckle.

3. The hand-held fluorescent quantitative PCR circulating tumor cell analyzer according to claim 1, characterized in that: The top of the gun body (12) is embedded with a touch screen (14), and the rear of the openable hatch (13) is provided with a USB-C data interface (15).

4. The hand-held fluorescent quantitative PCR circulating tumor cell analyzer according to claim 1, characterized in that: The grip (11) has a built-in lithium battery (16) and an anti-slip ripple on its outer surface.

5. A handheld real-time PCR circulating tumor cell analyzer according to claim 1, characterized in that: The gun body (12) has heat dissipation fins on its main side wall.

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