Denitrification nutrient solution enrichment culture device

Abstract

The utility model relates to the technical field of strain culture, specifically is a kind of nitrogen removal nutrient solution enrichment culture device, including shell, culture body and injection component, the shell is slidably installed with multiple culture bodies in circumferential array, and injection component is installed at the axis of shell, the injection component includes rotating matrix, drive body, injection tube and injection main pipe, rotating matrix is rotatably installed at the outer inner axis, the drive body of the culture body of the shell top fixed mounting drives rotating matrix, injection tube is horizontally fixedly installed in rotating matrix, and injection main pipe is sealingly rotatably installed at the other end of injection tube;Solve the problem that the existing nitrogen removal nutrient solution enrichment culture device is prone to concentration difference to affect culture effect due to uneven injection of nutrient solution.

Description

Technical Field

[0001] This utility model relates to the field of microbial culture technology, specifically a denitrification nutrient solution enrichment culture device. Background Technology

[0002] Existing denitrification nutrient solution enrichment and cultivation devices typically inject nutrient solution at only one or a few points in the reactor, such as adding it only on one side or in a corner. The nutrient solution initially accumulates around the injection point, forming a high-concentration core area, where COD levels exceed standards and ammonia nitrogen concentrations are 3-5 times higher than in other areas. Areas far from the injection point, such as the center, diagonals, or edges of the tank, rely on slow natural diffusion to reach the nutrients, resulting in delayed nutrient acquisition and a concentration gradient. This concentration gradient can easily lead to an imbalance in the microbial community, disrupting the competitive balance of the microbial community, causing the dominant bacteria to be replaced, and even triggering contamination by other microorganisms.

[0003] Therefore, this invention provides a denitrification nutrient solution enrichment culture device to solve the above problems. Utility Model Content

[0004] The technical problem to be solved by this utility model is that the existing denitrification nutrient solution enrichment culture device is prone to affecting the culture effect due to uneven injection of nutrient solution and the resulting concentration difference.

[0005] This utility model provides the following technical solution: a denitrification nutrient solution enrichment culture device, including a shell, culture bodies and an injection assembly. Multiple culture bodies are slidably installed in a circumferential array inside the shell. An injection assembly is installed at the axis of the shell. The injection assembly includes a rotating base, a driving body, an injection tube and an injection main tube. The rotating base is rotatably installed at the axis of the outer and inner parts. A driving body that drives the rotating base is fixedly installed on the top of the shell. An injection tube is horizontally fixedly installed inside the rotating base. The other end of the injection tube is sealed and rotatably installed with an injection main tube.

[0006] The injection tube includes a vertical tube and a horizontal tube. A coaxial vertical tube is rotatably installed above the main injection tube. Multiple horizontal tubes are fixedly connected to the end of the vertical tube along the circumference. An injection body is fixedly installed below the horizontal tube.

[0007] Multiple injectors are arrayed along the horizontal tubes, and the radius distance between the droppers on different horizontal tubes is different.

[0008] A jacket is fixedly installed on the surface of the injection tube, and a heat exchange tube is spirally fixedly installed inside the jacket.

[0009] Fins are fixedly installed inside the jacket, and the fins are attached to the side wall of the culture medium.

[0010] There are at least two horizontal tubes at different heights along the axis of the vertical tube.

[0011] The beneficial effects of this utility model are as follows: This invention injects nutrient solution sequentially into multiple culture cells in a circular array by rotating the injection assembly. By injecting denitrifying nutrient solution at different gradients through different radial distances and axial heights, the uniformity of the denitrifying nutrient solution injection is improved, which is beneficial to improving the enrichment culture effect. At the same time, during the injection of nutrient solution, the heat exchanger can control the temperature of the nutrient solution and the culture cells, thereby matching the appropriate temperature for the bacterial community and avoiding inhibition or inactivation of bacterial community activity, which is beneficial to improving the enrichment culture effect. Attached Figure Description

[0012] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0013] Figure 1 This is a frontal cross-sectional view of the overall structure of this utility model.

[0014] In the diagram: 1. Shell; 2. Culture body; 3. Injection assembly; 31. Rotating substrate; 32. Drive body; 33. Injection tube; 331. Vertical tube; 332. Horizontal tube; 333. Injection body; 34. Main injection tube; 4. Jacket; 5. Heat exchange tube; 6. Fin; 61. Outer end face; 62. Intermediate body; 63. Inner end face. Detailed Implementation

[0015] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Therefore, the following detailed description of the embodiments of this utility model is not intended to limit the scope of the claimed utility model, but merely represents some embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0016] It should be noted that similar labels and letters in the following figures indicate similar items. Therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

[0017] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," "outer," and "back side," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of this utility model is conventionally placed during use. These terms are used only for the convenience of describing this utility model and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation; therefore, they should not be construed as limitations on this utility model.

[0018] It should also be noted that, in the description of this utility model, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0019] Based on the problem that existing denitrification nutrient solution enrichment culture devices are prone to affecting culture results due to uneven nutrient solution injection and resulting concentration differences, such as... Figure 1 As shown, this embodiment of the present disclosure provides a denitrification nutrient solution enrichment culture device, including a shell 1, culture bodies 2, and an injection assembly 3. Multiple culture bodies 2 are slidably mounted in a circumferential array inside the shell 1. The injection assembly 3 is mounted at the axis of the shell 1. The injection assembly 3 includes a rotating base 31, a driving body 32, an injection tube 33, and an injection main tube 34. The rotating base 31 is rotatably mounted at the axis of the outer and inner parts. The driving body 32 for driving the rotating base 31 is fixedly mounted on the top of the shell 1. The injection tube 33 is horizontally fixedly mounted inside the rotating base 31. The other end of the injection tube 33 is sealed and rotatably mounted with the injection main tube 34.

[0020] like Figure 1 As shown, during the enrichment culture process using denitrified nutrient solution, the denitrified nutrient solution is input from the injection main pipe 34 and delivered to the injection tube 33. At the same time, the driving body 32 drives the rotating substrate 31 to rotate, thereby driving the injection tube 33 to rotate. This allows the denitrified nutrient solution to be input into the culture bodies 2 with different circumferences. This enables the continuous addition of denitrified nutrient solution during the logarithmic growth phase when microorganisms multiply rapidly, ensuring that the nutrient concentration remains stable at a suitable threshold. This avoids the problem of insufficient nutrient solution leading to insufficient nutrient solution consumption and inhibiting microbial culture caused by the rapid growth of microorganisms.

[0021] Culture 2 can be pulled out or pushed in by pushing or pulling, thereby removing culture 2 to load the initial bacterial population or collect the bacterial population after the enrichment culture is completed, or loading culture 2 into culture 2 for enrichment culture.

[0022] like Figure 1 As shown, the injection tube 33 includes a vertical tube 331 and a horizontal tube 332. The vertical tube 331 is rotatably mounted on the upper part of the injection tube 34. Multiple horizontal tubes 332 are fixedly connected to the end of the vertical tube 331 along the circumferential direction. An injection body 333 is fixedly mounted below the horizontal tube 332.

[0023] The denitrification nutrient solution is input through the injection main pipe 34 and transported sequentially along the vertical pipe 331 and the horizontal pipe 332, thereby being injected into the culture body 2 by the injection body 333 below the horizontal pipe 332.

[0024] like Figure 1 As shown, the lower end of the vertical tube 331 is connected to the injection main tube 34 and is installed in a sealed and rotatable manner. The upper end of the vertical tube 331 is fixedly connected to the inner wall of the rotating base 31. The horizontal tube 332 is fixedly installed on the inner wall surface of the rotating base 31.

[0025] While the driving body 32 drives the rotating substrate 31 to rotate, it also drives the horizontal tube 332 and the vertical tube 331 to rotate synchronously. This allows the horizontal tube 332 and the injection body 333 to inject denitrification nutrient solution into different culture bodies 2 in sequence during the rotation process, thereby replenishing the consumed nutrient solution and ensuring the stability of the microbial enrichment culture effect.

[0026] It should also be noted that during the initial stage of cultivation, the injection of denitrifying nutrient solution is initiated intermittently. During the proliferation phase in the middle stage of cultivation, the injection of denitrifying nutrient solution is initiated continuously.

[0027] The injector 333 uses an existing dropper or atomizing nozzle to inject denitrification nutrient solution in the form of drops or large-scale water mist.

[0028] like Figure 1 As shown, multiple injection bodies 333 are arrayed along the horizontal tube 332, and the radius distance of the injection bodies 333 on different horizontal tubes 332 is different.

[0029] Multiple injectors 333 with different radii can inject along the circumference at different radial positions during the rotation of the horizontal tube 332 and the injectors 333, thereby improving the uniformity of the denitrification nutrient solution injection and improving the enrichment culture effect.

[0030] like Figure 1 As shown, at least two horizontal tubes 332 are at different heights along the axis of the vertical tube 331.

[0031] The injector 333 uses an atomizing nozzle, which allows for complementary and adaptive spraying of the denitrification nutrient solution over a wide area at different heights. This avoids excessive concentration of nutrients in certain areas, improves the uniformity of the denitrification nutrient solution distribution, and enhances the enrichment culture effect.

[0032] When the injector 333 uses a dripper, the nutrient solution slowly dissolves and diffuses on the surface of the water / biofilm, maintaining a stable nutrient concentration within the optimal growth range for the bacterial community and ensuring continuous and efficient metabolism. The dripping at different heights allows for multi-gradient injection of denitrifying nutrient solution to match synergistic aerobic or anaerobic processes. Specifically: Adding water droplets from a relatively high height, such as 20-50cm above the liquid surface, will result in a brief contact with air during the droplet's descent, introducing a small amount of dissolved oxygen. It should be noted that this small amount of dissolved oxygen only increases the surface DO (0.2-0.5mg / L) without disrupting the overall gradient. Furthermore, nutrients will first diffuse to the surface / middle layer, which is the main habitat for aerobic nitrifying bacteria, allowing for direct replenishment of nitrogen and phosphorus sources.

[0033] Adding water droplets from a low height, such as 1-5 cm above the liquid surface or close to the bottom of the biofilm, results in almost no air contact between the droplets and the amount of dissolved oxygen introduced, which is negligible. Nutrients directly enter the bottom anaerobic zone. This bottom anaerobic zone is the habitat of denitrifying bacteria, allowing for precise replenishment of organic carbon sources and preventing them from being consumed by surface aerobic bacteria.

[0034] like Figure 1 As shown, a jacket 4 is fixedly installed on the surface of the injection tube 34, and a heat exchange tube 5 is spirally fixedly installed inside the jacket 4.

[0035] The heat exchange tube 5 allows for heat exchange with the nutrient solution during the injection process via the injection tube 34, thereby controlling the input temperature of the nutrient solution. This enables precise matching of the bacterial community's adaptation temperature, preventing inhibition or inactivation of bacterial activity and improving the enrichment culture effect.

[0036] It should be noted that the heat exchange tube 5 uses existing conduits and carries heat exchange medium such as water inside. The input and output ends of the heat exchange tube 5 are both on the lower side of the outer shell 1. This is existing technology and will not be described in detail.

[0037] like Figure 1 As shown, a fin 6 is fixedly installed inside the jacket 4, and the fin 6 is attached to the side wall of the culture body 2.

[0038] The fins 6 can conduct heat to the surface of the culture medium 2, thereby controlling the temperature of the nutrient solution to be at a uniform and suitable temperature while keeping the temperature of the culture medium 2 at the temperature that the microbial community is adapted to. This further facilitates the precise matching of the microbial community's adaptation temperature, avoids the inhibition or inactivation of microbial community activity, and helps to improve the enrichment culture effect.

[0039] like Figure 1As shown, the fin 6 includes an outer end face 61, an intermediate body 62 and an inner end face 63. The outer end face 61 is in contact with the culture body 2, and the inner end face 63 is in contact with the heat exchange tube 5. Both the outer end face 61 and the inner end face 63 have a fan-shaped structure. An intermediate body 62 that penetrates the jacket 4 is fixedly installed between the inner end face 63 and the outer end face 61.

[0040] like Figure 1 As shown, the cross-section of the outer end face 61 is an L-shaped structure.

[0041] The outer end face 61 of the L-shaped structure can simultaneously contact the side and bottom of the culture body 2, thereby increasing the contact area and thus increasing the temperature control effect. This further facilitates the precise matching of the temperature to which the bacterial community adapts, avoids inhibition or inactivation of bacterial community activity, and helps to improve the enrichment culture effect.

[0042] The enrichment culture process using denitrified nutrient solution in this embodiment is as follows: The operator first pulls out culture body 2 and sets the initial bacterial population inside, then pushes culture body 2 back in to start the culture.

[0043] During the cultivation process, the denitrified nutrient solution is introduced through the injection main pipe 34 and sequentially transported along the vertical pipe 331 and horizontal pipe 332, thereby being injected into the culture medium 2 by the injection body 333 below the horizontal pipe 332. Simultaneously, the driving body 32 drives the rotating substrate 31 to rotate, causing the horizontal pipe 332 and vertical pipe 331 to rotate synchronously. This allows the horizontal pipe 332 and injection body 333 to sequentially inject the denitrified nutrient solution into different culture mediums 2 during rotation, replenishing the consumed nutrient solution and ensuring the stability of the microbial enrichment culture.

[0044] Among them, the injector 333 injects denitrification nutrient solution at different gradients with different radial distances and axial heights, thereby improving the uniformity of denitrification nutrient solution injection and improving the enrichment culture effect.

[0045] During the process of the denitrified nutrient solution being introduced along the injection pipe 34, the heat exchange tube 5 exchanges heat with the denitrified nutrient solution in the injection pipe 34, thereby controlling the temperature of the denitrified nutrient solution to match the bacterial community. At the same time, the heat exchange tube 5 can also contact the culture body 2 through the fins 6, thereby controlling the temperature of the culture body 2 to match the bacterial community and increasing the contact area, avoiding inhibition or inactivation of bacterial community activity, which is conducive to improving the enrichment culture effect.

[0046] After successively passing through the initial initiation period, the middle proliferation period, and the later stabilization period of the bacterial community, the enrichment culture using denitrified nutrient solution was completed, and then the culture body 2 was taken out for use or preservation.

[0047] Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A denitrification nutrient solution enrichment culture device, comprising a shell (1), culture bodies (2), and an injection assembly (3), wherein multiple culture bodies (2) are slidably mounted in a circumferential array inside the shell (1), and the injection assembly (3) is mounted at the axial center of the shell (1), characterized in that: The injection assembly (3) includes a rotating base (31), a drive body (32), an injection tube (33), and an injection main tube (34). The rotating base (31) is rotatably mounted at the center of the housing (1). The drive body (32) for driving the rotating base (31) is fixedly mounted on the top of the housing (1). The injection tube (33) is horizontally fixedly mounted inside the rotating base (31). The injection main tube (34) is rotatably mounted at the other end of the injection tube (33).

2. The denitrification nutrient solution enrichment culture device according to claim 1, characterized in that: The injection tube (33) includes a vertical tube (331) and a horizontal tube (332). A coaxial vertical tube (331) is rotatably installed above the injection tube (34). Multiple horizontal tubes (332) are fixedly connected to the end of the vertical tube (331) along the circumferential direction. An injection body (333) is fixedly installed below the horizontal tube (332).

3. The denitrification nutrient solution enrichment culture device according to claim 2, characterized in that: Multiple injectors (333) are arrayed along the horizontal tube (332), and the radius distance of the injectors (333) on different horizontal tubes (332) is different.

4. The denitrification nutrient solution enrichment culture device according to claim 3, characterized in that: At least two horizontal tubes (332) are at different heights along the axis of the vertical tube (331).

5. The denitrification nutrient solution enrichment culture device according to claim 4, characterized in that: A jacket (4) is fixedly installed on the surface of the injection tube (34), and a heat exchange tube (5) is spirally fixed inside the jacket (4).

6. The denitrification nutrient solution enrichment culture device according to claim 5, characterized in that: A fin (6) is fixedly installed inside the jacket (4), and the fin (6) is attached to the side wall of the culture body (2).

7. The denitrification nutrient solution enrichment culture device according to claim 6, characterized in that: The cross-section of the fin (6) is L-shaped.

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